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caffeine</title> <meta name="description" content="Search results for: caffeine"> <meta name="keywords" content="caffeine"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="caffeine"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 41</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: caffeine</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">41</span> Electrochemical Determination of Caffeine Content in Ethiopian Coffee Samples Using Lignin Modified Glassy Carbon Electrode</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Meareg%20Amare">Meareg Amare</a>, <a href="https://publications.waset.org/abstracts/search?q=Senait%20Aklog"> Senait Aklog</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lignin film was deposited at the surface of the glassy carbon electrode potential-statically. In contrast to the unmodified glassy carbon electrode, an oxidative peak with an improved current and overpotential for caffeine at the modified electrode showed catalytic activity of the modifier towards oxidation of caffeine. Linear dependence of peak current on caffeine concentration in the range 6 × 10⁻⁶ to 100 × 10⁻⁶ mol L⁻¹ with determination coefficient and method detection limit (LoD = 3 s/slope) of 0.99925 and 8.37 × 10⁻⁷ mol L⁻¹, respectively, supplemented by recovery results of 93.79–102.17%, validated the developed method. An attempt was made to determine the caffeine content of aqueous coffee extracts of Ethiopian coffees grown in four coffee cultivating localities (Wonbera, Wolega, Finoteselam, and Zegie) and hence to evaluate the correlation between users preference and caffeine content. In agreement with reported works, caffeine contents (w/w%) of 0.164 in Wonbera coffee; 0.134 in Wolega coffee; 0.097 in Finoteselam coffee; and 0.089 in Zegie coffee were detected, confirming the applicability of the developed method for determination of caffeine in a complex matrix environment. The result indicated that users’ highest preference for Wonbera and least preference for Zegie cultivated coffees are in agreement with the caffeine content. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electrochemical" title="electrochemical">electrochemical</a>, <a href="https://publications.waset.org/abstracts/search?q=lignin" title=" lignin"> lignin</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=electrode" title=" electrode"> electrode</a> </p> <a href="https://publications.waset.org/abstracts/147641/electrochemical-determination-of-caffeine-content-in-ethiopian-coffee-samples-using-lignin-modified-glassy-carbon-electrode" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147641.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">119</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">40</span> The Effects of Prolonged Use of Caffeine on Thyroid and Adrenal Glands – A Retrospective Cohort Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vasishtha%20Avadhani%20Upadrasta">Vasishtha Avadhani Upadrasta</a>, <a href="https://publications.waset.org/abstracts/search?q=Mradul%20Kumar%20Daga"> Mradul Kumar Daga</a>, <a href="https://publications.waset.org/abstracts/search?q=Smita%20Kaushik"> Smita Kaushik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Caffeine consumption has skyrocketed in the recent decades as we try to match pace with the machines. Studies have been conducted on animals and a few on humans, mainly on the acute effects of high-dose caffeine intake. Almost none have been conducted on the chronic effects of caffeine consumption. This study involved Medical professionals as case subjects, who consumed caffeine daily. Methods: This study, over a period of 3 months, involved 96 volunteers (chosen randomly w.r.t. gender and field in medical fraternity), including people who drank >500mg of caffeine a day to people who consumed none. People with any co-morbidities at all were excluded straight away. Two sets of blood samples were drawn and assessed. Three groups were created, Group 1 (>200mg caffeine/day) and Group 2 (15-200 mg caffeine/day) and Group 3 (<200mg Caffeine/day). Results: The result of the study found that exposure to caffeine at doses >200mg/day for more than 6 months leads to a significant difference in circulating free T3 [(-0.96 pmol/L ± 0.07) = (-18.5%), CI 95%, p = .000024] and Cortisol [(-123 nmol/L ± 9.8) = (-46.8%), CI 95%, p = .00029] hormones but shows an insignificant effect on circulating TSH [0.4 mIU/L, CI 95%, p=.37] and ACTH [(-3.2 pg/ml ± 0.3), CI 95%, p = .53) hormones, which stay within normal physiological ranges, irrespective of the daily dose of consumption. Results also highlight that women are more susceptible to decrement in fT3 than men (Relative Risk =1.58, ANOVA F-static = 7.15, p = 0.0105). Conclusions: Caffeine consumption in excess of 200mg/day, for more than or equal to 6 months, causes significant derangement in basal fT3 and Cortisol hormone levels, without affecting the TSH and ACTH (regulatory) hormone levels, indicating disturbance of action at the peripheral and/or cellular levels, possibly via the Paraventricular Nucleus –Leptin-CAR-Adenosine interactions. Women are more susceptible to decrement in fT3 levels than men (at same dose of caffeine). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ACTH" title="ACTH">ACTH</a>, <a href="https://publications.waset.org/abstracts/search?q=adrenals" title=" adrenals"> adrenals</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=cortisol" title=" cortisol"> cortisol</a>, <a href="https://publications.waset.org/abstracts/search?q=thyroid" title=" thyroid"> thyroid</a>, <a href="https://publications.waset.org/abstracts/search?q=thyroxin" title=" thyroxin"> thyroxin</a>, <a href="https://publications.waset.org/abstracts/search?q=TSH" title=" TSH"> TSH</a> </p> <a href="https://publications.waset.org/abstracts/178244/the-effects-of-prolonged-use-of-caffeine-on-thyroid-and-adrenal-glands-a-retrospective-cohort-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/178244.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">74</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">39</span> The acute effects caffeine on testosterone and cortisol in young football players after One Session Anaerobic exercise </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Rostami">S. Rostami</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20H.%20Hosseini"> S. H. Hosseini</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Torabi"> A. A. Torabi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Bekhradi"> M. Bekhradi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Interest in the use of caffeine as an ergogenic aid has increased since the International Olympic Committee lifted the partial ban on its use. Caffeine has beneficial effects on various aspects of athletic performance, but its effects on training have been neglected. The purpose of this study was to investigate the acute effect of caffeine on testosterone and cortisole in young futsal players. Methods: Twenty-four professional futsal players with 18.3± 1.9 years ingested caffeine doses of 0, 200 and 800 mg in random order 1 hr before an anaerobic-exercise session (RAST test). Samples were taken at the time of caffeine ingestion and 30 min after the session. Data were log-transformed to estimate percent effects with mixed modeling, and effects were standardized to assess magnitudes. fects on training have been neglected. Results: Testosterone concentration showed a small increase of 15% (90% confidence limits, ± 19%) during exercise. Caffeine raised this concentration in a dose-dependent manner by a further small 21% (± 24%) at the highest dose. The 800-mg dose also produced a moderate 52% (± 44%) increase in cortisol. The effect of caffeine on the testosterone: cortisol ratio was a small decline (14%; ± 21%). Discussion and Conclusion: Caffeine has some potential to benefit training outcomes via the anabolic effects of the increase in testosterone concentration, but this benefit might be counteracted by the opposing catabolic effects of the increase in cortisol and resultant decline in the testosterone: cortisol ratio. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anabolic" title="anabolic">anabolic</a>, <a href="https://publications.waset.org/abstracts/search?q=catabolic" title=" catabolic"> catabolic</a>, <a href="https://publications.waset.org/abstracts/search?q=performance" title=" performance"> performance</a>, <a href="https://publications.waset.org/abstracts/search?q=testosterone" title=" testosterone"> testosterone</a>, <a href="https://publications.waset.org/abstracts/search?q=cortisol%20ratio" title=" cortisol ratio"> cortisol ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=RAST%20test" title=" RAST test "> RAST test </a> </p> <a href="https://publications.waset.org/abstracts/19021/the-acute-effects-caffeine-on-testosterone-and-cortisol-in-young-football-players-after-one-session-anaerobic-exercise" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19021.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">440</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">38</span> Quantitative Analysis of Caffeine in Pharmaceutical Formulations Using a Cost-Effective Electrochemical Sensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Y.%20T.%20Gebreslassie">Y. T. Gebreslassie</a>, <a href="https://publications.waset.org/abstracts/search?q=Abrha%20Tadesse"> Abrha Tadesse</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20C.%20Saini"> R. C. Saini</a>, <a href="https://publications.waset.org/abstracts/search?q=Rishi%20Pal"> Rishi Pal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Caffeine, known chemically as 3,7-dihydro-1,3,7-trimethyl-1H-purine-2,6-dione, is a naturally occurring alkaloid classified as an N-methyl derivative of xanthine. Given its widespread use in coffee and other caffeine-containing products, it is the most commonly consumed psychoactive substance in everyday human life. This research aimed to develop a cost-effective, sensitive, and easily manufacturable sensor for the detection of caffeine. Antraquinone-modified carbon paste electrode (AQMCPE) was fabricated, and the electrochemical behavior of caffeine on this electrode was investigated using cyclic voltammetry (CV) and square wave voltammetry (SWV) in a solution of 0.1M perchloric acid at pH 0.56. The modified electrode displayed enhanced electrocatalytic activity towards caffeine oxidation, exhibiting a two-fold increase in peak current and an 82 mV shift of the peak potential in the negative direction compared to an unmodified carbon paste electrode (UMCPE). Exploiting the electrocatalytic properties of the modified electrode, SWV was employed for the quantitative determination of caffeine. Under optimized experimental conditions, a linear relationship between peak current and concentration was observed within the range of 2.0 x 10⁻⁶ to 1.0× 10⁻⁴ M, with a correlation coefficient of 0.998 and a detection limit of 1.47× 10⁻⁷ M (signal-to-noise ratio = 3). Finally, the proposed method was successfully applied to the quantitative analysis of caffeine in pharmaceutical formulations, yielding recovery percentages ranging from 95.27% to 106.75%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antraquinone-modified%20carbon%20paste%20electrode" title="antraquinone-modified carbon paste electrode">antraquinone-modified carbon paste electrode</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=detection" title=" detection"> detection</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20sensor" title=" electrochemical sensor"> electrochemical sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=quantitative%20analysis" title=" quantitative analysis"> quantitative analysis</a> </p> <a href="https://publications.waset.org/abstracts/180289/quantitative-analysis-of-caffeine-in-pharmaceutical-formulations-using-a-cost-effective-electrochemical-sensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/180289.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">65</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">37</span> Effects of Extract from Lactuca sativa on Sleep in Pentobarbital-Induced Sleep and Caffeine-Induced Sleep Disturbance in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hae%20Dun%20Kim">Hae Dun Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Joo%20Hyun%20Jang"> Joo Hyun Jang</a>, <a href="https://publications.waset.org/abstracts/search?q=Geu%20Rim%20Seo"> Geu Rim Seo</a>, <a href="https://publications.waset.org/abstracts/search?q=Kyung%20Soo%20Ra"> Kyung Soo Ra</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyung%20Joo%20Suh"> Hyung Joo Suh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lactuca sativa (lettuce) has been known for its medical property to relieve anxiety and nervous. This study was implemented to investigate sleep-promoting effects of the lettuce alcohol extract (LAE). Caffeine is widely used psychoactive substance known to induced wakefulness and insomnia to its consumers. In the present study, the sedative-hypnotic activity of the LAE was studied using the method of pentobarbital-induced sleep in the mouse model. The LAE was administrated to mice 30 min before the pentobarbital injection. The LAE prolonged the pentobarbital-induced sleep duration and decreased sleep latency. The effects of LAE were comparable to those of induced by diazepam. Another study was performed to examine whether LAE ameliorates caffeine-induced sleep disturbance in mice. Additionally, caffeine (10 mg/kg, p.o) delayed sleep onset and reduced sleep duration of mice. Conversely, LAE treatment (80 or 160 mg/kg, p.o), especially at 160 mg/kg, normalized the sleep disturbance induced by caffeine. LAE supplementation can counter the sleep disturbance induced by caffeine. These results suggest that LAE possess significant sedative-hypnotic activity, which supports the popular use of lettuce for treatment of insomnia and provide the basis for new drug discovery. Furthermore, these results demonstrate that the lettuce extract may be preferable for the treatment of insomnia. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=caffeine" title="caffeine">caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=Lactuca%20sativa" title=" Lactuca sativa"> Lactuca sativa</a>, <a href="https://publications.waset.org/abstracts/search?q=sleep%20duration" title=" sleep duration"> sleep duration</a>, <a href="https://publications.waset.org/abstracts/search?q=sleep%20latency" title=" sleep latency"> sleep latency</a> </p> <a href="https://publications.waset.org/abstracts/49766/effects-of-extract-from-lactuca-sativa-on-sleep-in-pentobarbital-induced-sleep-and-caffeine-induced-sleep-disturbance-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/49766.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">306</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">36</span> The Effects of Different Doses of Caffeine on Young Futsal Players</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saead%20Rostami">Saead Rostami</a>, <a href="https://publications.waset.org/abstracts/search?q=Seyyed%20Hadi%20Hosseini%20Alavije"> Seyyed Hadi Hosseini Alavije</a>, <a href="https://publications.waset.org/abstracts/search?q=Aliakbar%20Torabi"> Aliakbar Torabi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Bekhradi"> Mohammad Bekhradi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study is about The effects of different doses of caffeine on young Futsal players. Young futsal players of selected ShahinShahr(a city in Esfahan province, Iran) team are sampled (24 people of 18.3±1.9 year- old). All players are members of youth team playing in Esfahan counties league. Having at least 5 years of experience, 2 practices and 1 match per week and lacking any limitation in the past 6 months are the most important requirements for sampling the players. Next, the study topic, its method, its uses, as ell possible risks are explained to the players. They signed a consent letter to take part in the study. Interest in the use of caffeine as an ergogenic aid has increased since the International Olympic Committee lifted the partial ban on its use. Caffeine has beneficial effects on various aspects of athletic performance, but its effects on training have been neglected. The purpose of this study was to investigate the acute effect of caffeine on testosterone and cortisole in young futsal players. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anabolic" title="anabolic">anabolic</a>, <a href="https://publications.waset.org/abstracts/search?q=catabolic" title=" catabolic"> catabolic</a>, <a href="https://publications.waset.org/abstracts/search?q=performance" title=" performance"> performance</a>, <a href="https://publications.waset.org/abstracts/search?q=testosterone%20cortisol%20ratio" title=" testosterone cortisol ratio"> testosterone cortisol ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=RAST%20test" title=" RAST test"> RAST test</a> </p> <a href="https://publications.waset.org/abstracts/46162/the-effects-of-different-doses-of-caffeine-on-young-futsal-players" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46162.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">347</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">35</span> Optimized Simultaneous Determination of Theobromine and Caffeine in Fermented and Unfermented Cacao Beans and in Cocoa Products Using Step Gradient Solvent System in Reverse Phase HPLC</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ian%20Marc%20G.%20Cabugsa">Ian Marc G. Cabugsa</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20Ryan%20A.%20Won"> Kim Ryan A. Won</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fast, reliable and simultaneous HPLC analysis of theobromine and caffeine in cacao and cocoa products was optimized in this study. The samples tested were raw, fermented, and roasted cacao beans as well as commercially available cocoa products. The HPLC analysis was carried out using step gradient solvent system with acetonitrile and water buffered with H3PO4 as the mobile phase. The HPLC system was optimized using 273 nm wavelength at 35 °C for the column temperature with a flow rate of 1.0 mL/min. Using this method, the theobromine percent recovery mean, Limit of Detection (LOD) and Limit of Quantification (LOQ) is 118.68(±3.38)%, 0.727 and 1.05 respectively. The percent recovery mean, LOD and LOQ for caffeine is 105.53(±3.25)%, 2.42 and 3.50 respectively. The inter-day and intra-day precision for theobromine is 4.31% and 4.48% respectively, while 7.02% and 7.03% was for caffeine respectively. Compared to the standard method in AOAC using methanol in isocratic solvent system, the results of the study produced lesser chromatogram noise with emphasis on theobromine and caffeine. The method is readily usable for cacao and cocoa substances analyses using HPLC with step gradient capability. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cacao" title="cacao">cacao</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=step%20gradient%20solvent%20system" title=" step gradient solvent system"> step gradient solvent system</a>, <a href="https://publications.waset.org/abstracts/search?q=theobromine" title=" theobromine"> theobromine</a> </p> <a href="https://publications.waset.org/abstracts/43155/optimized-simultaneous-determination-of-theobromine-and-caffeine-in-fermented-and-unfermented-cacao-beans-and-in-cocoa-products-using-step-gradient-solvent-system-in-reverse-phase-hplc" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43155.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">34</span> Association of Caffeine Consumption in Coffee, Tea and Soft Drinks with Age of Menopause</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Julita%20D.%20L.%20Nainggolan">Julita D. L. Nainggolan</a>, <a href="https://publications.waset.org/abstracts/search?q=Cindy%20Novita%20Ongkowijoyo"> Cindy Novita Ongkowijoyo</a>, <a href="https://publications.waset.org/abstracts/search?q=Veli%20Sungono"> Veli Sungono</a>, <a href="https://publications.waset.org/abstracts/search?q=Dyana%20Safitri%20Velies"> Dyana Safitri Velies</a>, <a href="https://publications.waset.org/abstracts/search?q=Ernestine%20Vivie%20Sadeli"> Ernestine Vivie Sadeli</a>, <a href="https://publications.waset.org/abstracts/search?q=Jimmy"> Jimmy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Normal menstrual cycle in women ranges from 21-34 days. Menopause is defined as the time when there have been no menstrual periods for 12 consecutive months and no other biological or physiological cause can be identified. Caffeine might increase the estradiol in the early of follicular phase and possibly increase the progesterone and shorten menstruation cycle. Women with shorter menstrual cycle, (below 26 days) would likely get to menopause 1.4 years earlier than those who are normal, and 2.2 years earlier than women with longer menstrual cycle. Purpose: To study the association of caffeine consumption in coffee, tea, and soft drinks with the age of menopause. Design Study: A cross-sectional study using purposive sampling of 132 menopause women from elderly nursing, hospitals and students’ relatives from August 2015-December 2015. The mean difference of age of menopause among the caffeine intake was analyzed by using the unpaired t-test and logistic regression. Results: Mean current age of the respondents are 61.4 years ± SD 9.8; and age of menopause was 47.7 years ± SD 4.2. There are 49.6% who drink coffee, 62.6% of tea and 7.6% of soft drinks. The analysis of t-test showed no significant mean difference in age of menopause among women who drink coffee, tea and soft drinks, mean age of 47.63 ± 4.3 in coffee with p=0.392, mean age of 47.8 ± 4 in tea with p=0.373; and mean age of 46 ± 5.5 with p=0.083 after adjustment of smoking history. Conclusion: Consumption of caffeine among women who drink coffee, tea, and soft drinks did not show significant mean difference in age of menopause. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=caffeine" title="caffeine">caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=menopause" title=" menopause"> menopause</a>, <a href="https://publications.waset.org/abstracts/search?q=coffee" title=" coffee"> coffee</a>, <a href="https://publications.waset.org/abstracts/search?q=tea" title=" tea"> tea</a>, <a href="https://publications.waset.org/abstracts/search?q=soda" title=" soda"> soda</a>, <a href="https://publications.waset.org/abstracts/search?q=soft%20drinks" title=" soft drinks"> soft drinks</a> </p> <a href="https://publications.waset.org/abstracts/63810/association-of-caffeine-consumption-in-coffee-tea-and-soft-drinks-with-age-of-menopause" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63810.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">239</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">33</span> The Possible Interaction between Bisphenol A, Caffeine and Epigallocatechin-3-Gallate on Neurotoxicity Induced by Manganese in Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Azza%20A.%20Ali">Azza A. Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Hebatalla%20I.%20Ahmed"> Hebatalla I. Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Asmaa%20Abdelaty"> Asmaa Abdelaty</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Manganese (Mn) is a naturally occurring element. Exposure to high levels of Mn causes neurotoxic effects and represents an environmental risk factor. Mn neurotoxicity is poorly understood but changing of AChE activity, monoamines and oxidative stress has been established. Bisphenol A (BPA) is a synthetic compound widely used in the production of polycarbonate plastics. There is considerable debate about whether its exposure represents an environmental risk. Caffeine is one of the major contributors to the dietary antioxidants which prevent oxidative damage and may reduce the risk of chronic neurodegenerative diseases. Epigallocatechin-3-gallate is another major component of green tea and has known interactions with caffeine. It also has health-promoting effects in CNS. Objective: To evaluate the potential protective effects of Caffeine and/or EGCG against Mn-induced neurotoxicity either alone or in the presence of BPA in rats. Methods: Seven groups of rats were used and received daily for 5 weeks MnCl2.4H2O (10 mg/kg, IP) except the control group which received saline, corn oil and distilled H2O. Mn was injected either alone or in combination with each of the following: BPA (50 mg/kg, PO), caffeine (10 mg/kg, PO), EGCG (5 mg/kg, IP), caffeine + EGCG and BPA +caffeine +EGCG. All rats were examined in five behavioral tests (grid, bar, swimming, open field and Y- maze tests). Biochemical changes in monoamines, caspase-3, PGE2, GSK-3B, glutamate, acetyl cholinesterase and oxidative parameters, as well as histopathological changes in the brain, were also evaluated for all groups. Results: Mn significantly increased MDA and nitrite content as well as caspase-3, GSK-3B, PGE2 and glutamate levels while significantly decreased TAC and SOD as well as cholinesterase in the striatum. It also decreased DA, NE and 5-HT levels in the striatum and frontal cortex. BPA together with Mn enhanced oxidative stress generation induced by Mn while increased monoamine content that was decreased by Mn in rat striatum. BPA abolished neuronal degeneration induced by Mn in the hippocampus but not in the substantia nigra, striatum and cerebral cortex. Behavioral examinations showed that caffeine and EGCG co-administration had more pronounced protective effect against Mn-induced neurotoxicity than each one alone. EGCG alone or in combination with caffeine prevented neuronal degeneration in the substantia nigra, striatum, hippocampus and cerebral cortex induced by Mn while caffeine alone prevented neuronal degeneration in the substantia nigra and striatum but still showed some nuclear pyknosis in cerebral cortex and hippocampus. The marked protection of caffeine and EGCG co-administration also confirmed by the significant increase in TAC, SOD, ACHE, DA, NE and 5-HT as well as the decrease in MDA, nitrite, caspase-3, PGE2, GSK-3B, the glutamic acid in the striatum. Conclusion: Neuronal degeneration induced by Mn showed some inhibition with BPA exposure despite the enhancement in oxidative stress generation. Co-administration of EGCG and caffeine can protect against neuronal degeneration induced by Mn and improve behavioral deficits associated with its neurotoxicity. The protective effect of EGCG was more pronounced than that of caffeine even with BPA co-exposure. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=manganese" title="manganese">manganese</a>, <a href="https://publications.waset.org/abstracts/search?q=bisphenol%20a" title=" bisphenol a"> bisphenol a</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=epigallocatechin-3-gallate" title=" epigallocatechin-3-gallate"> epigallocatechin-3-gallate</a>, <a href="https://publications.waset.org/abstracts/search?q=neurotoxicity" title=" neurotoxicity"> neurotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=behavioral%20tests" title=" behavioral tests"> behavioral tests</a>, <a href="https://publications.waset.org/abstracts/search?q=rats" title=" rats"> rats</a> </p> <a href="https://publications.waset.org/abstracts/62763/the-possible-interaction-between-bisphenol-a-caffeine-and-epigallocatechin-3-gallate-on-neurotoxicity-induced-by-manganese-in-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/62763.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">228</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">32</span> The in vitro Effects of Various Immunomodulatory Nutritional Compounds on Antigen-Stimulated Whole-Blood Culture Cytokine Production</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayu%20S.%20Muhamad">Ayu S. Muhamad</a>, <a href="https://publications.waset.org/abstracts/search?q=Michael%20Gleeson"> Michael Gleeson</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Immunomodulators are substances that alter immune system via dynamic regulation of messenger molecules. It can be divided into immunostimulant and immunosuppressant. It can help to increase immunity of people with a low immune system, and also can help to normalize an overactive immune system. Aim of this study is to investigate the effects of in vitro exposure to low and high doses of several immunomodulators which include caffeine, kaloba and quercetin on antigen-stimulated whole blood culture cytokine production. Whole blood samples were taken from 5 healthy males (age: 32 ± 12 years; weight: 75.7 ± 6.1 kg; BMI: 24.3 ± 1.5 kg/m2) following an overnight fast with no vigorous activity during the preceding 24 h. The whole blood was then stimulated with 50 µl of 100 x diluted Pediacel vaccine and low or high dose of immunomodulators in the culture plate. After 20 h incubation (5% CO2, 37°C), it was analysed using the Evidence Investigator to determine the production of cytokines including IL-2, IL-4, IL-10, IFN-γ, and IL-1α. Caffeine and quercetin showed a tendency towards decrease cytokine production as the doses were increased. On the other hand, an upward trend was evident with kaloba, where a high dose of kaloba seemed to increase the cytokine production. In conclusion, we found that caffeine and quercetin have potential as immunosuppressant and kaloba as immunostimulant. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=caffeine" title="caffeine">caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokine" title=" cytokine"> cytokine</a>, <a href="https://publications.waset.org/abstracts/search?q=immunomodulators" title=" immunomodulators"> immunomodulators</a>, <a href="https://publications.waset.org/abstracts/search?q=kaloba" title=" kaloba"> kaloba</a>, <a href="https://publications.waset.org/abstracts/search?q=quercetin" title=" quercetin"> quercetin</a> </p> <a href="https://publications.waset.org/abstracts/11601/the-in-vitro-effects-of-various-immunomodulatory-nutritional-compounds-on-antigen-stimulated-whole-blood-culture-cytokine-production" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11601.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">466</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">31</span> Analysis of Pharmaceuticals in Influents of Municipal Wastewater Treatment Plants in Jordan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=O.%20A.%20Al-Mashaqbeh">O. A. Al-Mashaqbeh</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20M.%20Ghrair"> A. M. Ghrair</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Alsafadi"> D. Alsafadi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20S.%20Dalahmeh"> S. S. Dalahmeh</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20L.%20Bartelt-Hunt"> S. L. Bartelt-Hunt</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20D.%20Snow"> D. D. Snow</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Grab samples were collected in the summer to characterize selected pharmaceuticals and personal care products (PPCPs) in the influent of two wastewater treatment plants (WWTPs) in Jordan. Liquid chromatography tandem mass spectrometry (LC–MS/MS) was utilized to determine the concentrations of 18 compounds of PPCPs. Among all of the PPCPs analyzed, eight compounds were detected in the influent samples (1,7-dimethylxanthine, acetaminophen, caffeine, carbamazepine, cotinine, morphine, sulfamethoxazole and trimethoprim). However, five compounds (amphetamine, cimetidine, diphenhydramine, methylenedioxyamphetamine (MDA) and sulfachloropyridazine) were not detected in collected samples (below the detection limits <0.005 µg/l). Moreover, the results indicated that the highest concentration levels detected in collected samples were caffeine, acetaminophen, 1,7-dimethylxanthine, cotinine and carbamazepine at concentration of 182.5 µg/L, 28.7 µg/l, 7.47 µg/l, 4.67 µg/l and 1.54 µg/L, respectively. In general, most of compounds concentrations measured in wastewater in Jordan are within the range for wastewater previously reported in India wastewater except caffeine. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pharmaceuticals" title="pharmaceuticals">pharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=personal%20care%20products" title=" personal care products"> personal care products</a>, <a href="https://publications.waset.org/abstracts/search?q=wastewater" title=" wastewater"> wastewater</a>, <a href="https://publications.waset.org/abstracts/search?q=Jordan" title=" Jordan"> Jordan</a> </p> <a href="https://publications.waset.org/abstracts/92947/analysis-of-pharmaceuticals-in-influents-of-municipal-wastewater-treatment-plants-in-jordan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/92947.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">30</span> Comparative Economic Evaluation of Additional Respiratory Resources Utilized after Methylxanthine Initiation for the Treatment of Apnea of Prematurity in a South Asian Country</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shivakumar%20M">Shivakumar M</a>, <a href="https://publications.waset.org/abstracts/search?q=Leslie%20Edward%20S%20Lewis"> Leslie Edward S Lewis</a>, <a href="https://publications.waset.org/abstracts/search?q=Shashikala%20Devadiga"> Shashikala Devadiga</a>, <a href="https://publications.waset.org/abstracts/search?q=Sonia%20Khurana"> Sonia Khurana</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Methylxanthines are used for the treatment of AOP, to facilitate extubation and as a prophylactic agent to prevent apnea. Though the popularity of Caffeine has risen, it is expensive in a resource constrained developing countries like India. Objective: To evaluate the cost-effectiveness of Caffeine compared with Aminophylline treatment for AOP with respect to additional ventilatory resource utilized in different birth weight categorization. Design, Settings and Participants – Single centered, retrospective economic evaluation was done. Participants included preterm newborns with < 34 completed weeks of gestation age that were recruited under an Indian Council of Medical Research funded randomized clinical trial. Per protocol data was included from Neonatal Intensive Care Unit, Kasturba Hospital, Manipal, India between April 2012 and December 2014. Exposure: Preterm neonates were randomly allocated to either Caffeine or Aminophylline as per the trial protocol. Outcomes and Measures – We assessed surfactant requirement, duration of Invasive and Non-Invasive Ventilation, Total Methylxanthine cost and additional cost for respiratory support bared by the payers per day during hospital stay. For the purpose of this study Newborns were stratified as Category A – < 1000g, Category B – 1001 to 1500g and Category C – 1501 to 2500g. Results: Total 146 (Caffeine -72 and Aminophylline – 74) babies with Mean ± SD gestation age of 29.63 ± 1.89 weeks were assessed. 32.19% constitute of Category A, 55.48% were B and 12.33% were C. The difference in median duration of additional NIV and IMV support was statistically insignificant. However 60% of neonates who received Caffeine required additional surfactant therapy (p=0.02). The total median (IQR) cost of Caffeine was significantly high with Rs.10535 (Q3-6317.50, Q1-15992.50) where against Aminophylline cost was Rs.352 (Q3-236, Q1-709) (p < 0.001). The additional costs spent on respiratory support per day in neonates on either Methylxanthines were found to be statistically insignificant in the entire weight based category of our study. Whereas in Category B, the median O2 charges per day were found to have more in Caffeine treated newborns (p=0.05) with border line significance. In category A, providing one day NIV or IMV support significantly increases the unit log cost of Caffeine by 13.6% (CI – 95% ranging from 4 to 24; p=0.005) over log cost of Aminophylline. Conclusion: Cost of Caffeine is expensive than Aminophylline. It was found to be equally efficacious in reducing the number duration of NIV or IMV support. However adjusted with the NIV and IMV days of support, neonates fall in category A and category B who were on Caffeine pays excess amount of respiratory charges per day over aminophylline. In perspective of resource poor settings Aminophylline is cost saving and economically approachable. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=methylxanthines%20include%20caffeine%20and%20aminophylline" title="methylxanthines include caffeine and aminophylline">methylxanthines include caffeine and aminophylline</a>, <a href="https://publications.waset.org/abstracts/search?q=AOP%20%28apnea%20of%20prematurity%29" title=" AOP (apnea of prematurity)"> AOP (apnea of prematurity)</a>, <a href="https://publications.waset.org/abstracts/search?q=IMV%20%28invasive%20mechanical%20ventilation%29" title=" IMV (invasive mechanical ventilation)"> IMV (invasive mechanical ventilation)</a>, <a href="https://publications.waset.org/abstracts/search?q=NIV%20%28non%20invasive%20ventilation%29" title=" NIV (non invasive ventilation)"> NIV (non invasive ventilation)</a>, <a href="https://publications.waset.org/abstracts/search?q=category%20a%20%E2%80%93%20%3C1000g" title=" category a – <1000g"> category a – <1000g</a>, <a href="https://publications.waset.org/abstracts/search?q=category%20b%20%E2%80%93%201001%20to%201500g%20and%20category%20c%20%E2%80%93%201501%20to%202500g" title=" category b – 1001 to 1500g and category c – 1501 to 2500g"> category b – 1001 to 1500g and category c – 1501 to 2500g</a> </p> <a href="https://publications.waset.org/abstracts/35733/comparative-economic-evaluation-of-additional-respiratory-resources-utilized-after-methylxanthine-initiation-for-the-treatment-of-apnea-of-prematurity-in-a-south-asian-country" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35733.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">433</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">29</span> HPLC-UV Screening of Legal (Caffeine and Yohimbine) and Illegal (Ephedrine and Sibutramine) Substances from Weight Loss Dietary Supplements for Athletes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amelia%20Tero-Vescan">Amelia Tero-Vescan</a>, <a href="https://publications.waset.org/abstracts/search?q=Camil-Eugen%20Vari"> Camil-Eugen Vari</a>, <a href="https://publications.waset.org/abstracts/search?q=Laura%20Ciulea"> Laura Ciulea</a>, <a href="https://publications.waset.org/abstracts/search?q=Cristina%20Filip"> Cristina Filip</a>, <a href="https://publications.waset.org/abstracts/search?q=Silvia%20Imre"> Silvia Imre</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A HPLC –UV method for the identification of ephedrine (EPH), sibutramine (SB), yohimbine (Y) and caffeine (CF) was developed. Separation was performed on a Kromasil 100-RP8, 150 mm x 4.6 mm, 5 mm column equipped with a precolumn Kromasil RP 8. Mobile phase was a gradient of 80-35 % sodium dihydrogen phosphate pH=5 with NH4OH and acetonitrile over 15 minutes time of analysis. Based on the responses of 113 athletes about dietary supplements (DS) consumed for "fat burning" and weight loss which have a legal status in Romania, 28 supplements have been selected and investigated for their content in CF, Y, legal substances, and SB, EPH (prohibited substances in DS). The method allows quantitative determination of the four substances in a short analysis time and with minimum cost. The presence of SB and EPH in the analyzed DS was not detected while the content in CF and Y considering the dosage recommended by the manufacturer does not affect the health of the consumers. DS labeling (plant extracts with CF and Y content) allows manufacturers to avoid declaring correct and exact amounts per pharmaceutical form (pure CF or equivalent and Y, respectively). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dietary%20supplements" title="dietary supplements">dietary supplements</a>, <a href="https://publications.waset.org/abstracts/search?q=sibutramine" title=" sibutramine"> sibutramine</a>, <a href="https://publications.waset.org/abstracts/search?q=ephedrine" title=" ephedrine"> ephedrine</a>, <a href="https://publications.waset.org/abstracts/search?q=yohimbine" title=" yohimbine"> yohimbine</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a> </p> <a href="https://publications.waset.org/abstracts/2518/hplc-uv-screening-of-legal-caffeine-and-yohimbine-and-illegal-ephedrine-and-sibutramine-substances-from-weight-loss-dietary-supplements-for-athletes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2518.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">442</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28</span> Compositional Analysis and Antioxidant Activities of the Chocolate Fermented by Lactobacillus plantarum CK10</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hye%20Rim%20Kang">Hye Rim Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=So%20Yae%20Koh"> So Yae Koh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ji-Yeon%20Ryu"> Ji-Yeon Ryu</a>, <a href="https://publications.waset.org/abstracts/search?q=Chang%20Kyu%20Lee"> Chang Kyu Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Ji%20Hee%20Lim"> Ji Hee Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyeon%20A.%20Kim"> Hyeon A. Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Geun%20Hyung%20Im"> Geun Hyung Im</a>, <a href="https://publications.waset.org/abstracts/search?q=Somi%20Kim%20Cho"> Somi Kim Cho</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, antioxidant properties and compositional analysis of fermented chocolate were examined. Chocolate was fermented with Lactobacillus plantarum CK10. As fermentation time went by, pH was decreased (5.26±0.02 to 3.98±0.06) while titratable acidity was increased (5.36±0.19 to 13.31±0.34). The total polyphenol contents were maintained through the fermentation. The contents of total polyphenol were slightly increased at 8 hr (6.34±0.12 mg GAE (Gallic acid equivalent)/g), and it reached to comparable levels of the control at 24 hr (control, 5.47±0.36 mg GAE/g); 24 hr, 5.19±0.23 mg GAE/g). Similarly, the total flavonoid contents were not significantly changed during fermentation. The pronounced radical scavenging activities of chocolate, against DPPH-, ABTS-, and Alkyl radical, were observed. The levels of antioxidant activities were not dramatically altered in the course of fermentation. By gas chromatography-mass spectrometry analysis, the increase in lactic acid was measured and four major compounds, HMF, xanthosine, caffeine, and theobromine, were identified. The relative peak area of caffeine and theobromine was considerably changed during fermentation. However, no significant difference in the levels of caffeine and theobromine were observed by high-performance liquid chromatography analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title="antioxidant">antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=chocolate" title=" chocolate"> chocolate</a>, <a href="https://publications.waset.org/abstracts/search?q=compositional%20analysis" title=" compositional analysis"> compositional analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=Lactobaillus%20plantarum" title=" Lactobaillus plantarum"> Lactobaillus plantarum</a> </p> <a href="https://publications.waset.org/abstracts/55853/compositional-analysis-and-antioxidant-activities-of-the-chocolate-fermented-by-lactobacillus-plantarum-ck10" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/55853.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">287</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27</span> Knowledge and Eating Behavior of Teenage Pregnancy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Udomporn%20Yingpaisuk">Udomporn Yingpaisuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Premwadee%20Karuhadej"> Premwadee Karuhadej</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The purposed of this research was to study the eating habit of teenage pregnancy and its relationship to the knowledge of nutrition during pregnancy. The 100 samples were derived from simple random sampling technique of the teenage pregnancy in Bangkae District. The questionnaire was used to collect data with the reliability of 0.8. The data were analyzed by SPSS for Windows with multiple regression technique. Percentage, mean and the relationship of knowledge of eating and eating behavior were obtained. The research results revealed that their knowledge in nutrition was at the average of 4.07 and their eating habit that they mentioned most was to refrain from alcohol and caffeine at 82% and the knowledge in nutrition influenced their eating habits at 54% with the statistically significant level of 0.001. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=teenage%20pregnancy" title="teenage pregnancy">teenage pregnancy</a>, <a href="https://publications.waset.org/abstracts/search?q=knowledge%20of%20eating" title=" knowledge of eating"> knowledge of eating</a>, <a href="https://publications.waset.org/abstracts/search?q=eating%20behavior" title=" eating behavior"> eating behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=alcohol" title=" alcohol"> alcohol</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine "> caffeine </a> </p> <a href="https://publications.waset.org/abstracts/9773/knowledge-and-eating-behavior-of-teenage-pregnancy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/9773.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">358</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">26</span> Use and Health Effects of Caffeinated Beverages in Omani Students</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nasiruddin%20Khan">Nasiruddin Khan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The increased use of caffeinated beverages and energy drink is posing threat to all ages and gender especially, younger adults. There is a lack of scientific evidence in Oman regarding caffeine and energy drink consumption. Our study aims to demonstrate the prevalence, pattern, knowledge and awareness, and side effects of caffeine intake among university students. This cross-sectional study including (N=365) apparently healthy male and female Omani university students aged 18-30 years, was carried out from February 2018-June 2018. A self-administered questionnaire with various sections was used to obtain information. The prevalence of caffeinated beverage consumption was commonly high among participants (97%). The males preferred Nescafe, coffee (both p < 0.001), espresso (p < 0.022), and soda (p < 0.008) while females consumed more tea (p < 0.029). The awareness about negative health impact of caffeine intake was significantly higher in females rather than males (p < 0.002). The overall prevalence of energy drink consumption was 42.1% (n=149), and higher in males (75%, p < 0.001). More males consumed 3-5 and > 5 cans/day while females used 1-2 cans/day. The starting age of energy drink use was higher in females (16-20 years (51.1%)) as compared to males (11-15 years (33.3%)). Females were more aware of caffeine as energy drink ingredient (p < 0.036) than males. The major source of information about enery drink was family and friends (58.3%). Red Bull was the commonly used brand (55.5%) among participants. Common reasons for high energy drink consumption were energy boost (68.4 %), taste (62.9%), reduce fatigue (52.1%), and better performance (47.3%). Females reported breathing problem, and abnormal heart beat (p < 0.004, 0.054, respectively), while more males reported irritability than females (p < 0.052). The prevalence of caffeinated beverage and energy drink consumption is high among participants. The awareness, and knowledge among university student is not satisfactory and needs immediate action to avoid excess use of such consumption. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=energy%20drink" title="energy drink">energy drink</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeinated%20beverages" title=" caffeinated beverages"> caffeinated beverages</a>, <a href="https://publications.waset.org/abstracts/search?q=awareness" title=" awareness"> awareness</a>, <a href="https://publications.waset.org/abstracts/search?q=Oman" title=" Oman"> Oman</a> </p> <a href="https://publications.waset.org/abstracts/104348/use-and-health-effects-of-caffeinated-beverages-in-omani-students" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104348.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">128</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">25</span> Determination of the Phytochemicals Composition and Pharmacokinetics of whole Coffee Fruit Caffeine Extract by Liquid Chromatography-Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Boris%20Nemzer">Boris Nemzer</a>, <a href="https://publications.waset.org/abstracts/search?q=Nebiyu%20Abshiru"> Nebiyu Abshiru</a>, <a href="https://publications.waset.org/abstracts/search?q=Z.%20B.%20Pietrzkowski"> Z. B. Pietrzkowski</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Coffee cherry is one of the most ubiquitous agricultural commodities which possess nutritional and human health beneficial properties. Between the two most widely used coffee cherries Coffea arabica (Arabica) and Coffea canephora (Robusta), Coffea arabica remains superior due to its sensory properties and, therefore, remains in great demand in the global coffee market. In this study, the phytochemical contents and pharmacokinetics of Coffeeberry® Energy (CBE), a commercially available Arabica whole coffee fruit caffeine extract, are investigated. For phytochemical screening, 20 mg of CBE was dissolved in an aqueous methanol solution for analysis by mass spectrometry (MS). Quantification of caffeine and chlorogenic acids (CGAs) contents of CBE was performed using HPLC. For the bioavailability study, serum samples were collected from human subjects before and after 1, 2 and 3 h post-ingestion of 150mg CBE extract. Protein precipitation and extraction were carried out using methanol. Identification of compounds was performed using an untargeted metabolomic approach on Q-Exactive Orbitrap MS coupled to reversed-phase chromatography. Data processing was performed using Thermo Scientific Compound Discover 3.3 software. Phytochemical screening identified a total of 170 compounds, including organic acids, phenolic acids, CGAs, diterpenoids and hydroxytryptamine. Caffeine & CGAs make up more than, respectively, 70% & 9% of the total CBE composition. For serum samples, a total of 82 metabolites representing 32 caffeine- and 50 phenolic-derived metabolites were identified. Volcano plot analysis revealed 32 differential metabolites (24 caffeine- and 8 phenolic-derived) that showed an increase in serum level post-CBE dosing. Caffeine, uric acid, and trimethyluric acid isomers exhibited 4- to 10-fold increase in serum abundance post-dosing. 7-Methyluric acid, 1,7-dimethyluric acid, paraxanthine and theophylline exhibited a minimum of 1.5-fold increase in serum level. Among the phenolic-derived metabolites, iso-feruloyl quinic acid isomers (3-, 4- and 5-iFQA) showed the highest increase in serum level. These compounds were essentially absent in serum collected before dosage. More interestingly, the iFQA isomers were not originally present in the CBE extract, as our phytochemical screen did not identify these compounds. This suggests the potential formation of the isomers during the digestion and absorption processes. Pharmacokinetics parameters (Cmax, Tmax and AUC0-3h) of caffeine- and phenolic-derived metabolites were also investigated. Caffeine was rapidly absorbed, reaching a maximum concentration (Cmax) of 10.95 µg/ml in just 1 hour. Thereafter, caffeine level steadily dropped from the peak level, although it did not return to baseline within the 3-hour dosing period. The disappearance of caffeine from circulation was mirrored by the rise in the concentration of its methylxanthine metabolites. Similarly, serum concentration of iFQA isomers steadily increased, reaching maximum (Cmax: 3-iFQA, 1.54 ng/ml; 4-iFQA, 2.47 ng/ml; 5-iFQA, 2.91 ng/ml) at tmax of 1.5 hours. The isomers remained well above the baseline during the 3-hour dosing period, allowing them to remain in circulation long enough for absorption into the body. Overall, the current study provides evidence of the potential health benefits of a uniquely formulated whole coffee fruit product. Consumption of this product resulted in a distinct serum profile of bioactive compounds, as demonstrated by the more than 32 metabolites that exhibited a significant change in systemic exposure. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phytochemicals" title="phytochemicals">phytochemicals</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmacokinetics" title=" pharmacokinetics"> pharmacokinetics</a>, <a href="https://publications.waset.org/abstracts/search?q=differential%20metabolites" title=" differential metabolites"> differential metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=chlorogenic%20acids" title=" chlorogenic acids"> chlorogenic acids</a> </p> <a href="https://publications.waset.org/abstracts/160767/determination-of-the-phytochemicals-composition-and-pharmacokinetics-of-whole-coffee-fruit-caffeine-extract-by-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/160767.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">68</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">24</span> Effect of Radioprotectors on DNA Repair Enzyme and Survival of Gamma-Irradiated Cell Division Cycle Mutants of Saccharomyces pombe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Purva%20Nemavarkar">Purva Nemavarkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Badri%20Narain%20Pandey"> Badri Narain Pandey</a>, <a href="https://publications.waset.org/abstracts/search?q=Jitendra%20Kumar"> Jitendra Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: The objective was to understand the effect of various radioprotectors on DNA damage repair enzyme and survival in gamma-irradiated wild and cdc mutants of S. pombe (fission yeast) cultured under permissive and restrictive conditions. DNA repair process, as influenced by radioprotectors, was measured by activity of DNA polymerase in the cells. The use of single cell gel electrophoresis assay (SCGE) or Comet Assay to follow gamma-irradiation induced DNA damage and effect of radioprotectors was employed. In addition, studying the effect of caffeine at different concentrations on S-phase of cell cycle was also delineated. Materials and Methods: S. pombe cells grown at permissive temperature (250C) and/or restrictive temperature (360C) were followed by gamma-radiation. Percentage survival and activity of DNA Polymerase (yPol II) were determined after post-irradiation incubation (5 h) with radioprotectors such as Caffeine, Curcumin, Disulphiram, and Ellagic acid (the dose depending on individual D 37 values). The gamma-irradiated yeast cells (with and without the radioprotectors) were spheroplasted by enzyme glusulase and subjected to electrophoresis. Radio-resistant cells were obtained by arresting cells in S-phase using transient treatment of hydroxyurea (HU) and studying the effect of caffeine at different concentrations on S-phase of cell cycle. Results: The mutants of S. pombe showed insignificant difference in survival when grown under permissive conditions. However, growth of these cells under restrictive temperature leads to arrest in specific phases of cell cycle in different cdc mutants (cdc10: G1 arrest, cdc22: early S arrest, cdc17: late S arrest, cdc25: G2 arrest). All the cdc mutants showed decrease in survival after gamma radiation when grown at permissive and restrictive temperatures. Inclusion of the radioprotectors at respective concentrations during post irradiation incubation showed increase in survival of cells. Activity of DNA polymerase enzyme (yPol II) was increased significantly in cdc mutant cells exposed to gamma-radiation. Following SCGE, a linear relationship was observed between doses of irradiation and the tail moments of comets. The radioprotection of the fission yeast by radioprotectors can be seen by the reduced tail moments of the yeast comets. Caffeine also exhibited its radio-protective ability in radio-resistant S-phase cells obtained after HU treatment. Conclusions: The radioprotectors offered notable radioprotection in cdc mutants when added during irradiation. The present study showed activation of DNA damage repair enzyme (yPol II) and an increase in survival after treatment of radioprotectors in gamma irradiated wild type and cdc mutants of S. pombe cells. Results presented here showed feasibility of applying SCGE in fission yeast to follow DNA damage and radioprotection at high doses, which are not feasible with other eukaryotes. Inclusion of caffeine at 1mM concentration to S phase cells offered protection and did not decrease the cell viability. It can be proved that at minimal concentration, caffeine offered marked radioprotection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=radiation%20protection" title="radiation protection">radiation protection</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20cycle" title=" cell cycle"> cell cycle</a>, <a href="https://publications.waset.org/abstracts/search?q=fission%20yeast" title=" fission yeast"> fission yeast</a>, <a href="https://publications.waset.org/abstracts/search?q=comet%20assay" title=" comet assay"> comet assay</a>, <a href="https://publications.waset.org/abstracts/search?q=s-phase" title=" s-phase"> s-phase</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20repair" title=" DNA repair"> DNA repair</a>, <a href="https://publications.waset.org/abstracts/search?q=radioprotectors" title=" radioprotectors"> radioprotectors</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=curcumin" title=" curcumin"> curcumin</a>, <a href="https://publications.waset.org/abstracts/search?q=SCGE" title=" SCGE"> SCGE</a> </p> <a href="https://publications.waset.org/abstracts/164879/effect-of-radioprotectors-on-dna-repair-enzyme-and-survival-of-gamma-irradiated-cell-division-cycle-mutants-of-saccharomyces-pombe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164879.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">113</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">23</span> Analysis of Total Acid in Arabica Coffee Beans after Fermentation with Ohmic Technology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reta">Reta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Coffee is widely consumed not only because of its typical taste, but coffee has antioxidant properties because of its polyphenols, and it stimulates brain's performance. The main problem with the consumption of coffee is its content of caffeine. Caffeine, when consumed in excess, can increase muscle tension, stimulate the heart, and increase the secretion of gastric acid. In this research, we applied ohmic-based fermentation technology, which is specially designed to mimic the stomach. We used Arabica coffee, which although cheaper than Luwak coffee, has high acidity, which needs to be reduced. Hence, we applied the ohmic technology, varied the time and temperature of the process and measured the total acidity of the coffee to determine optimum fermentation conditions. Results revealed total acidity of the coffee varied with fermentation conditions; 0.32% at 400C and 12 hr, and 0.52% at 400C and 6 hr. The longer the fermentation, the lower was the acidity. The acidity of the mongoose-fermented (natural fermentation) beans was 2.34%, which is substantially higher than the acidity of the ohmic samples. Ohmic-based fermentation technology, therefore, offers improvements in coffee quality, and this is discussed to highlight the potential of ohmic technology in coffee processing. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ohmic%20technology" title="ohmic technology">ohmic technology</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=coffee%20quality" title=" coffee quality"> coffee quality</a>, <a href="https://publications.waset.org/abstracts/search?q=Arabica%20coffee" title=" Arabica coffee"> Arabica coffee</a> </p> <a href="https://publications.waset.org/abstracts/56713/analysis-of-total-acid-in-arabica-coffee-beans-after-fermentation-with-ohmic-technology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56713.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">342</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">22</span> Optimization of Cacao Fermentation in Davao Philippines Using Sustainable Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ian%20Marc%20G.%20Cabugsa">Ian Marc G. Cabugsa</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20Ryan%20Won"> Kim Ryan Won</a>, <a href="https://publications.waset.org/abstracts/search?q=Kareem%20Mamac"> Kareem Mamac</a>, <a href="https://publications.waset.org/abstracts/search?q=Manuel%20Dee"> Manuel Dee</a>, <a href="https://publications.waset.org/abstracts/search?q=Merlita%20Garcia"> Merlita Garcia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An optimized cacao fermentation technique was developed for the cacao farmers of Davao City Philippines. Cacao samples with weights ranging from 150-250 kilograms were collected from various cacao farms in Davao City and Zamboanga City Philippines. Different fermentation techniques were used starting with design of the sweat box, prefermentation conditionings, number of days for fermentation and number of turns. As the beans are being fermented, its temperature was regularly monitored using a digital thermometer. The resultant cacao beans were assessed using physical and chemical means. For the physical assessment, the bean cut test, bean count tests, and sensory test were used. Quantification of theobromine, caffeine, and antioxidants in the form of equivalent quercetin was used for chemical assessment. Both the theobromine and caffeine were analyzed using HPLC method while the antioxidant was analyzed spectrometrically. To come up with the best fermentation procedure, the different assessment were given priority coefficients wherein the physical tests – taste test, cut, and bean count tests were given priority over the results of the chemical test. The result of the study was an optimized fermentation protocol that is readily adaptable and transferable to any cacao cooperatives or groups in Mindanao or even Philippines as a whole. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cacao" title="cacao">cacao</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=optimization" title=" optimization"> optimization</a>, <a href="https://publications.waset.org/abstracts/search?q=Philippines" title=" Philippines"> Philippines</a> </p> <a href="https://publications.waset.org/abstracts/64839/optimization-of-cacao-fermentation-in-davao-philippines-using-sustainable-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/64839.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">452</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">21</span> Determinaton of Processing Parameters of Decaffeinated Black Tea by Using Pilot-Scale Supercritical CO₂ Extraction</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saziye%20Ilgaz">Saziye Ilgaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Atilla%20Polat"> Atilla Polat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is a need for development of new processing techniques to ensure safety and quality of final product while minimizing the adverse impact of extraction solvents on environment and residue levels of these solvents in final product, decaffeinated black tea. In this study pilot scale supercritical carbon dioxide (SCCO₂) extraction was used to produce decaffeinated black tea in place of solvent extraction. Pressure (250, 375, 500 bar), extraction time (60, 180, 300 min), temperature (55, 62.5, 70 °C), CO₂ flow rate (1, 2 ,3 LPM) and co-solvent quantity (0, 2.5, 5 %mol) were selected as extraction parameters. The five factors BoxBehnken experimental design with three center points was performed to generate 46 different processing conditions for caffeine removal from black tea samples. As a result of these 46 experiments caffeine content of black tea samples were reduced from 2.16 % to 0 – 1.81 %. The experiments showed that extraction time, pressure, CO₂ flow rate and co-solvent quantity had great impact on decaffeination yield. Response surface methodology (RSM) was used to optimize the parameters of the supercritical carbon dioxide extraction. Optimum extraction parameters obtained of decaffeinated black tea were as follows: extraction temperature of 62,5 °C, extraction pressure of 375 bar, CO₂ flow rate of 3 LPM, extraction time of 176.5 min and co-solvent quantity of 5 %mol. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=supercritical%20carbon%20dioxide" title="supercritical carbon dioxide">supercritical carbon dioxide</a>, <a href="https://publications.waset.org/abstracts/search?q=decaffeination" title=" decaffeination"> decaffeination</a>, <a href="https://publications.waset.org/abstracts/search?q=black%20tea" title=" black tea"> black tea</a>, <a href="https://publications.waset.org/abstracts/search?q=extraction" title=" extraction"> extraction</a> </p> <a href="https://publications.waset.org/abstracts/58747/determinaton-of-processing-parameters-of-decaffeinated-black-tea-by-using-pilot-scale-supercritical-co2-extraction" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58747.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">364</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">20</span> Environmental Risk of Pharmaceuticals, Drugs of Abuse and Stimulant Caffeine in Marine Water: A Case Study in the North-Western of Spain</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Raquel%20Dafouz%20Neus%20C%C3%A1ceres">Raquel Dafouz Neus Cáceres</a>, <a href="https://publications.waset.org/abstracts/search?q=Javier%20Fernandez-Rubio"> Javier Fernandez-Rubio</a>, <a href="https://publications.waset.org/abstracts/search?q=Belinda%20Huerta%20Jos%C3%A9%20Luis%20Rodr%C3%ADguez-Gil"> Belinda Huerta José Luis Rodríguez-Gil</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicola%20Mastroianni"> Nicola Mastroianni</a>, <a href="https://publications.waset.org/abstracts/search?q=Miren%20L%C3%B3pez%20de%20Alda"> Miren López de Alda</a>, <a href="https://publications.waset.org/abstracts/search?q=Dami%C3%A0%20Barcel%C3%B3"> Damià Barceló</a>, <a href="https://publications.waset.org/abstracts/search?q=Yolanda%20Valc%C3%A1rcel"> Yolanda Valcárcel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The region of Galicia, found in north-western (NW) Spain, is a national and world leader in shellfish, especially mussel production, and recognized for its fishing industry. Few studies have evaluated the presence of emerging contaminants in NW Spain, with those published mainly concerning the continental aquatic environment. The objective of this study was to identify the environmental risk posed by the presence of pharmaceuticals and drugs of abuse in this important coastal region. The presence of sixteen pharmaceuticals (benzodiazepines, anxiolytics, and caffeine), and 19 drugs of abuse (cocainics, amphetamine-like compounds, opiates and opioids, lysergic compounds, and cannabinoids) was assessed in 23 sites located in the Rías (Coastal inlets) of Muros, Arousa, and Pontevedra (NW Spain). Twenty-two of these locations were affected by waste-water treatment plant (WWTP) effluents, and one represented the effluent of one of these WWTPs. Venlafaxine was the pharmaceutical compound detected at higher concentration in the three Rías, with a maximum value of 291 ng/L at the site Porto do Son (Ría de Muros). Total concentration in the three Rías was 819,26 ng/L. Next, citalopram and lorazepam were the most prevalent compounds detected. Metabolite of cocaine benzoylecgonine was the drug of abuse with the highest concentration, measured at 972 ng/L in the Ría of Noia WWTP (no dilution). This compound was also detected at 142 ng/L in the site La Isla de Aros, Ría of Pontevedra. Total concentration for the three Rías was 1210 ng/L. Ephedrine was also detected at high level in the three Rías, with a total concentration of 579,28 ng/L. The results obtained for caffeine show maximum and average concentrations of 857 ng/L Isla de Arosa, Ría de Pontevedra the highest measured in seawater in Spain. A preliminary hazard assessment was carried out by comparing these measured environmental concentrations (MEC) to predicted no-effect concentrations (PNECs) for aquatic organisms. Six out of the 22 seawater samples resulted in a Hazard Quotient (HQ) from chronic exposure higher than 1 with the highest being 17.14, indicating a high probability of adverse effects in the aquatic environment. In addition, the risk was assessed on the basis of persistence, bioaccumulation, and toxicity (PBT). This work was financially supported by the Spanish Ministry of Economy and Competitiveness through the Carlos III Health Institute and the program 'Proyectos de Investigacion en Salud 2015-2017' FIS (PI14/00516), the European Regional Development Fund (ERDF), the Catalan Government (Consolidated Research Groups '2014 SGR 418 - Water and Soil Quality Unit' and 2014 SGR 291 - ICRA), and the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 603437. The poster entitled 'Environmental Risk of Pharmaceuticals, Drugs of Abuse and Stimulant Caffeine in Marine Water: A Case Study in the North-Western of Spain'. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20of%20abuse" title="drug of abuse">drug of abuse</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceuticals" title=" pharmaceuticals"> pharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20risk" title=" environmental risk"> environmental risk</a>, <a href="https://publications.waset.org/abstracts/search?q=seawater" title=" seawater"> seawater</a> </p> <a href="https://publications.waset.org/abstracts/77766/environmental-risk-of-pharmaceuticals-drugs-of-abuse-and-stimulant-caffeine-in-marine-water-a-case-study-in-the-north-western-of-spain" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77766.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">217</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">19</span> Development of a Dairy Drink Made of Cocoa, Coffee and Orange By-Products with Antioxidant Activity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gianella%20Franco">Gianella Franco</a>, <a href="https://publications.waset.org/abstracts/search?q=Karen%20Suarez"> Karen Suarez</a>, <a href="https://publications.waset.org/abstracts/search?q=Mar%C3%ADa%20Quijano"> María Quijano</a>, <a href="https://publications.waset.org/abstracts/search?q=Patricia%20Manzano"> Patricia Manzano</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Agro-industries generate large amounts of waste, which are mostly untapped. This research was carried out to use cocoa, coffee and orange industrial by-products to develop a dairy drink. The product was prepared by making a 10% aqueous extract of the mixture of cocoa and coffee beans shells and orange peel. Extreme Vertices Mixture Design was applied to vary the proportions of the ingredients of the aqueous extract, getting 13 formulations. Each formulation was mixed with skim milk and pasteurized. The attributes of taste, smell, color and appearance were evaluated by a semi-trained panel by multiple comparisons test, comparing the formulations against a standard marked as "R", which consisted of a coffee commercial drink. The formulations with the highest scores were selected to maximize the Total Polyphenol Content (TPC) through a process of linear optimization resulting in the formulation 80.5%: 18.37%: 1.13% of cocoa bean shell, coffee bean shell and orange peel, respectively. The Total Polyphenol Content was 4.99 ± 0.34 mg GAE/g of drink, DPPH radical scavenging activity (%) was 80.14 ± 0.05 and caffeine concentration of 114.78 mg / L, while the coffee commercial drink presented 3.93 ± 0.84 mg GAE / g drink, 55.54 ± 0.03 % and 47.44 mg / L of TPC, DPPH radical scavenging activity and caffeine content, respectively. The results show that it is possible to prepare an antioxidant - rich drink with good sensorial attributes made of industrial by-products. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DPPH" title="DPPH">DPPH</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title=" polyphenols"> polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=waste" title=" waste"> waste</a>, <a href="https://publications.waset.org/abstracts/search?q=food%20science" title=" food science"> food science</a> </p> <a href="https://publications.waset.org/abstracts/21999/development-of-a-dairy-drink-made-of-cocoa-coffee-and-orange-by-products-with-antioxidant-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21999.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">467</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">18</span> Investigating University Students' Attitudes towards Infertility in Terms of Socio-Demographic Variables</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yelda%20Ka%C4%9Fn%C4%B1c%C4%B1">Yelda Kağnıcı</a>, <a href="https://publications.waset.org/abstracts/search?q=Se%C3%A7il%20Seymenler"> Seçil Seymenler</a>, <a href="https://publications.waset.org/abstracts/search?q=Bahar%20Baran"> Bahar Baran</a>, <a href="https://publications.waset.org/abstracts/search?q=Erol%20Esen"> Erol Esen</a>, <a href="https://publications.waset.org/abstracts/search?q=Bar%C4%B1%C5%9Fcan%20%C3%96zt%C3%BCrk"> Barışcan Öztürk</a>, <a href="https://publications.waset.org/abstracts/search?q=Ender%20Siyez"> Ender Siyez</a>, <a href="https://publications.waset.org/abstracts/search?q=Di%C4%9Fdem%20M.%20Siyez"> Diğdem M. Siyez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Infertility is the inability to reproduce after twelve months or longer unprotected sexual relationship. Although infertility is not a life threatening illness, it is considered as a serious problem for both the individual and the society. At this point, the importance of examining attitudes towards infertility is critical. Negative attitudes towards infertility may postpone individuals’ help seeking behaviors. The aim of this study is to investigate university students’ attitudes towards infertility in terms of socio-demographic variables (gender, age, taking sexual health education, existence of an infertile individual in the social network, plans about having child and behaviors about health). The sample of the study was 9693 university students attending to 21 universities in Turkey. Of the 9693 students, % 51.6 (n = 5002) were female, % 48.4 (n = 4691) were male. The data was collected by Attitudes toward Infertility Scale developed by researchers and Personal Information Form. In data analysis first frequencies were calculated, then in order to test whether there were significant differences in attitudes towards infertility scores of university students in terms of socio-demographic variables, one way ANOVA was conducted. According to the results, it was found that female students, students who had sexual health education, who have sexual relationship experience, who have an infertile individual in their social networks, who have child plans, who have high caffeine usage and who use alcohol regularly have more positive attitudes towards infertility. On the other hand, attitudes towards infidelity did not show significant differences in terms of age and cigarette usage. When the results of the study were evaluated in general, it was seen that university students’ attitudes towards infertility were negative. The attitudes of students who have high caffeine and alcohols usage were high. It can be considered that these students are aware that their social habits are risky. Female students’ positive attitudes might be explained by their gender role. The results point out that in order to decrease university students’ negative attitudes towards infertility, there is a necessity to develop preventive programs in universities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=infertility" title="infertility">infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=attitudes" title=" attitudes"> attitudes</a>, <a href="https://publications.waset.org/abstracts/search?q=sex" title=" sex"> sex</a>, <a href="https://publications.waset.org/abstracts/search?q=university%20students" title=" university students"> university students</a> </p> <a href="https://publications.waset.org/abstracts/74443/investigating-university-students-attitudes-towards-infertility-in-terms-of-socio-demographic-variables" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74443.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">247</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">17</span> Determination of the Element Contents in Turkish Coffee and Effect of Sugar Addition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20M.%20Fercan">M. M. Fercan</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20S.%20Kipcak"> A. S. Kipcak</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Dere%20Ozdemir"> O. Dere Ozdemir</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20B.%20Piskin"> M. B. Piskin</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Moroydor%20Derun"> E. Moroydor Derun</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Coffee is a widely consumed beverage with many components such as caffeine, flavonoids, phenolic compounds, and minerals. Coffee consumption continues to increase due to its physiological effects, its pleasant taste, and aroma. Robusta and Arabica are two basic types of coffee beans. The coffee bean used for Turkish coffee is Arabica. There are many elements in the structure of coffee and have various effect on human health such as Sodium (Na), Boron (B), Magnesium (Mg) and Iron (Fe). In this study, the amounts of Mg, Na, Fe, and B contents in Turkish coffee are determined and effect of sugar addition is investigated for conscious consumption. The analysis of the contents of coffees was determined by using inductively coupled plasma optical emission spectrometry (ICP-OES). From the results of the experiments the Mg, Na, Fe and B contents of Turkish coffee after sugar addition were found as 19.83, 1.04, 0.02, 0.21 ppm, while without using sugar these concentrations were found 21.46, 0.81, 0.008 and 0.16 ppm. In addition, element contents were calculated for 1, 3 and 5 cups of coffee in order to investigate the health effects. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=health%20effect" title="health effect">health effect</a>, <a href="https://publications.waset.org/abstracts/search?q=ICP-OES" title=" ICP-OES"> ICP-OES</a>, <a href="https://publications.waset.org/abstracts/search?q=sugar" title=" sugar"> sugar</a>, <a href="https://publications.waset.org/abstracts/search?q=Turkish%20coffee" title=" Turkish coffee"> Turkish coffee</a> </p> <a href="https://publications.waset.org/abstracts/36877/determination-of-the-element-contents-in-turkish-coffee-and-effect-of-sugar-addition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/36877.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">498</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">16</span> Multiclass Analysis of Pharmaceuticals in Fish and Shrimp Tissues by High-Performance Liquid Chromatography-Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reza%20Pashaei">Reza Pashaei</a>, <a href="https://publications.waset.org/abstracts/search?q=Reda%20Dzingelevi%C4%8Dien%C4%97"> Reda Dzingelevičienė</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An efficient, reliable, and sensitive multiclass analytical method has been expanded to simultaneously determine 15 human pharmaceutical residues in fish and shrimp tissue samples by ultra-high-performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise ten classes, namely analgesic, antibacterial, anticonvulsant, cardiovascular, fluoroquinolones, macrolides, nonsteroidal anti-inflammatory, penicillins, stimulant, and sulfonamide. A simple liquid extraction procedure based on 0.1% formic acid in methanol was developed. Chromatographic conditions were optimized, and mobile phase namely 0.1 % ammonium acetate (A), and acetonitrile (B): 0 – 2 min, 15% B; 2 – 5 min, linear to 95% B; 5 – 10 min, 95% B; and 10 – 12 min was obtained. Limits of detection and quantification ranged from 0.017 to 1.371 μg/kg and 0.051 to 4.113 μg/kg, respectively. Finally, amoxicillin, azithromycin, caffeine, carbamazepine, ciprofloxacin, clarithromycin, diclofenac, erythromycin, furosemide, ibuprofen, ketoprofen, naproxen, sulfamethoxazole, tetracycline, and triclosan were quantifiable in fish and shrimp samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fish" title="fish">fish</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid%20chromatography" title=" liquid chromatography"> liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceuticals" title=" pharmaceuticals"> pharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=shrimp" title=" shrimp"> shrimp</a>, <a href="https://publications.waset.org/abstracts/search?q=solid-phase%20extraction" title=" solid-phase extraction"> solid-phase extraction</a> </p> <a href="https://publications.waset.org/abstracts/143257/multiclass-analysis-of-pharmaceuticals-in-fish-and-shrimp-tissues-by-high-performance-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143257.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Transfer Rate of Organic Water Contaminants through a Passive Sampler Membrane of Polyethersulfone (PES) </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hamidreza%20Sharifan">Hamidreza Sharifan</a>, <a href="https://publications.waset.org/abstracts/search?q=Audra%20Morse"> Audra Morse</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Accurate assessments of contaminant concentrations based on traditional grab sampling methods are not always possible. Passive samplers offer an attractive alternative to traditional sampling methods that overcomes these limitations. The POCIS approach has been used as a screening tool for determining the presence/absence, possible sources and relative amounts of organic compounds at field sites. The objective for the present research is on mass transfer of five water contaminants (atrazine, caffeine, bentazon, ibuprofen, atenolol) through the Water Boundary Layer (WBL) and membrane. More specific objectives followed by establishing a relationship between the sampling rate and water solubility of the compounds, as well as comparing the molecular weight of the compounds and concentration of the compounds at the time of equilibrium. To determine whether water boundary layer effects transport rate through the membrane is another main objective in this paper. After GC mass analysis of compounds, regarding the WBL effect in this experiment, Sherwood number for the experimental tank developed. A close relationship between feed concentration of compound and sampling rate has been observed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=passive%20sampler" title="passive sampler">passive sampler</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20contaminants" title=" water contaminants"> water contaminants</a>, <a href="https://publications.waset.org/abstracts/search?q=PES-transfer%20rate" title=" PES-transfer rate"> PES-transfer rate</a>, <a href="https://publications.waset.org/abstracts/search?q=contaminant%20concentrations" title=" contaminant concentrations"> contaminant concentrations</a> </p> <a href="https://publications.waset.org/abstracts/43320/transfer-rate-of-organic-water-contaminants-through-a-passive-sampler-membrane-of-polyethersulfone-pes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43320.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">455</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Microbial Bioagent Triggered Biochemical Response in Tea (Camellia sinensis) Inducing Resistance against Grey Blight Disease and Yield Enhancement</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Popy%20Bora">Popy Bora</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20C.%20Bora"> L. C. Bora</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Bhattacharya"> A. Bhattacharya</a>, <a href="https://publications.waset.org/abstracts/search?q=Sehnaz%20S.%20Ahmed"> Sehnaz S. Ahmed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microbial bioagents, viz., Pseudomonas fluorescens, Bacillus subtilis, and Trichoderma viride were assessed for their ability to suppress grey blight caused by Pestalotiopsis theae, a major disease of tea crop in Assam. The expression of defense-related phytochemicals due to the application of these bioagents was also evaluated. The individual bioagents, as well as their combinations, were screened for their bioefficacy against P. theae in vitro using nutrient agar (NA) as basal medium. The treatment comprising a combination of the three bioagents, P. fluorescens, B. subtilis, and T. viride showed significantly the highest inhibition against the pathogen. Bioformulation of effective bioagent combinations was further evaluated under field condition, where significantly highest reduction of grey blight (90.30%), as well as the highest increase in the green leaf yield (10.52q/ha), was recorded due to application of the bioformulation containing the three bioagents. The application of the three bioformulation also recorded an enhanced level of caffeine (4.15%) and polyphenols (22.87%). A significant increase in the enzymatic activity of phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase were recorded in the plants treated with the microbial bioformulation of the three bioagents. The present investigation indicates the role of microbial agents in suppressing disease, inducing plant defense response, as well as improving the quality of tea. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=enzymatic%20activity" title="enzymatic activity">enzymatic activity</a>, <a href="https://publications.waset.org/abstracts/search?q=grey%20blight" title=" grey blight"> grey blight</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20bioagents" title=" microbial bioagents"> microbial bioagents</a>, <a href="https://publications.waset.org/abstracts/search?q=Pestalotiopsis%20theae" title=" Pestalotiopsis theae"> Pestalotiopsis theae</a>, <a href="https://publications.waset.org/abstracts/search?q=phytochemicals" title=" phytochemicals"> phytochemicals</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20defense" title=" plant defense"> plant defense</a>, <a href="https://publications.waset.org/abstracts/search?q=tea" title=" tea "> tea </a> </p> <a href="https://publications.waset.org/abstracts/113669/microbial-bioagent-triggered-biochemical-response-in-tea-camellia-sinensis-inducing-resistance-against-grey-blight-disease-and-yield-enhancement" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/113669.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">141</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Effect of Brewing on the Bioactive Compounds of Coffee</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ceyda%20Dadali">Ceyda Dadali</a>, <a href="https://publications.waset.org/abstracts/search?q=Ye%C5%9Fim%20Elmaci"> Yeşim Elmaci</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Coffee was introduced as an economic crop during the fifteenth century; nowadays it is the most important food commodity ranking second after crude oil. Desirable sensory properties make coffee one of the most often consumed and most popular beverages in the world. The coffee preparation method has a significant effect on flavor and composition of coffee brews. Three different extraction methodologies namely decoction, infusion and pressure methods have been used for coffee brew preparation. Each of these methods is related to specific granulation (coffee grind) of coffee powder, water-coffee ratio temperature and brewing time. Coffee is a mixture of 1500 chemical compounds. Chemical composition of coffee highly depends on brewing methods, coffee bean species and roasting time-temperature. Coffee contains a wide number of very important bioactive compounds, such as diterpenes: cafestol and kahweol, alkaloids: caffeine, theobromine and trigonelline, melanoidins, phenolic compounds. The phenolic compounds of coffee include chlorogenic acids (quinyl esters of hidroxycinnamic acids), caffeic, ferulic, p-coumaric acid. In coffee caffeoylquinic acids, feruloylquinic acids and di-caffeoylquinic acids are three main groups of chlorogenic acids constitues 6% -10% of dry weight of coffee. The bioavailability of chlorogenic acids in coffee depends on the absorption and metabolization to biomarkers in individuals. Also, the interaction of coffee polyphenols with other compounds such as dietary proteins affects the biomarkers. Since bioactive composition of coffee depends on brewing methods effect of coffee brewing method on bioactive compounds of coffee will be discussed in this study. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioactive%20compounds%20of%20coffee" title="bioactive compounds of coffee">bioactive compounds of coffee</a>, <a href="https://publications.waset.org/abstracts/search?q=biomarkers" title=" biomarkers"> biomarkers</a>, <a href="https://publications.waset.org/abstracts/search?q=coffee%20brew" title=" coffee brew"> coffee brew</a>, <a href="https://publications.waset.org/abstracts/search?q=effect%20of%20brewing" title=" effect of brewing "> effect of brewing </a> </p> <a href="https://publications.waset.org/abstracts/81620/effect-of-brewing-on-the-bioactive-compounds-of-coffee" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81620.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">196</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> In-Situ Fabrication of ZnO PES Membranes for Treatment of Pharmaceuticals</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Oranso%20T.%20Mahlangi">Oranso T. Mahlangi</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhekie%20B.%20Mamba"> Bhekie B. Mamba</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The occurrence of trace organic compounds (TOrCs) in water has raised health concerns for living organisms. The majority of TorCs, including pharmaceuticals and volatile organic compounds, are poorly monitored, partly due to the high cost of analysis and less strict water quality guidelines in South Africa. Therefore, the removal of TorCs is important to guarantee safe potable water. In this study, ZnO nanoparticles were fabricated in situ in polyethersulfone (PES) polymer solutions. This was followed by membrane synthesis using the phase inversion technique. Techniques such as FTIR, Raman, SEM, AFM, EDS, and contact angle measurements were used to characterize the membranes for several physicochemical properties. The membranes were then evaluated for their efficiency in treating pharmaceutical wastewater and resistance to organic (sodium alginate) and protein (bovine serum albumin) fouling. EDS micrographs revealed uniform distribution of ZnO nanoparticles within the polymer matrix, while SEM images showed uniform fingerlike structures. The addition of ZnO increased membrane roughness as well as hydrophilicity (which in turn improved water fluxes). The membranes poorly rejected monovalent and divalent salts (< 10%), making them resistant to flux decline due to concentration polarization effects. However, the membranes effectively removed carbamazepine, caffeine, sulfamethoxazole, ibuprofen, and naproxen by over 50%. ZnO PES membranes were resistant to organic and protein fouling compared to the neat membrane. ZnO PES ultrafiltration membranes may provide a solution in the reclamation of wastewater. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=trace%20organic%20compounds" title="trace organic compounds">trace organic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceuticals" title=" pharmaceuticals"> pharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=membrane%20fouling" title=" membrane fouling"> membrane fouling</a>, <a href="https://publications.waset.org/abstracts/search?q=wastewater%20reclamation" title=" wastewater reclamation"> wastewater reclamation</a> </p> <a href="https://publications.waset.org/abstracts/147068/in-situ-fabrication-of-zno-pes-membranes-for-treatment-of-pharmaceuticals" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147068.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=caffeine&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=caffeine&page=2" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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