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Serum-Free and Animal-Free Cell Culture Media | Bio-Techne
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class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a 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Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" 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data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Enhance the consistency of your cell cultures and smooth your progress into potential translational studies for regenerative medicine and cell therapy. Adopting defined media will also improve the safety profile of your cell product and simplify regulatory pathways.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> </div> </div> </div> </div> </div> </div> </div> </section> </div><!-- end hero-banner --> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/reagents">Bio-Techne Reagents</a> </li> <li> <a href="/reagents/cell-culture-reagents">Cell Culture Reagents</a> </li> <li> Serum-Free and Animal-Free Cell Culture </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Serum-Free and Animal-Free Cell Culture</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>The elimination of serum and animal components in culture media is an important step towards a defined cell culture process. Defined media contain only products and materials with known chemical identity such as small molecules, salts, and fatty acids. These media may also include recombinant proteins that are not produced in animal systems.</p> <p>Incorporating serum-free or animal-free media will benefit your cell culture process in several ways:</p> <ul> <li>Improving consistency by reducing variability in media composition</li> <li>Simplifying compliance with regulatory guidelines</li> <li>Simplifying comparability testing if raw material changes are required</li> <li>Reducing time-to-market for your cell product</li> </ul> <p>If you’re not ready for serum-free or animal-free culture, visit our <a href="/reagents/cell-culture-reagents">Cell Culture Reagents</a> page for classical media, serum products, and basement membrane extracts.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/3d-cell-culture-image-v2.jpg" alt="adherent cells supported by matrix proteins" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Interested in Testing an Animal-Free RUO Protein?</h2></div> <a href="/reagents/proteins/animal-free-proteins#sample-request" > <button type="button" class="btn btn-primary"> Request a Free Sample </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Serum-Free Cell Culture</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Serum-free cell culture media will improve your experimental consistency by reducing the number of unidentified culture components as well as the effects of unknown levels of vitamins, hormones, and growth factors. In addition, serum-free media</p> <ul> <li>Reduce the risk of potential viral contaminants that may be present in the serum</li> <li>Reduce the cost and inconvenience of batch testing sera</li> <li>Eliminate uncertainty about global supply fluctuations</li> <li>Simplify purification and downstream processing</li> </ul> <p>These media components do not require supplementation with fetal bovine serum (fetal calf serum; FBS) to support cell survival, proliferation, or function:</p> <ul> <li><a href="/t/neural-stem-cells/cell-culture">N-2 MAX and N21-MAX supplements</a> for NPC derivation, maintenance, and differentiation</li> <li><a href="/p/cell-culture/stemxvivo-serum-free-human-msc-expansion-media_ccm014">StemXVivo<sup>™</sup> Serum-Free Human MSC Expansion Media</a> and <a href="/p/cell-culture/stemxvivo-serum-free-msc-freezing-media_ccm016">Freezing Media</a></li> <li><a href="/p/cell-culture/stemxvivo-serum-free-human-t-cell-base-media_ccm010">StemXVivo Serum-Free Human T Cell Base Media</a></li> <li><a href="/p/cell-culture/stemxvivo-serum-free-tumorsphere-media_ccm012">StemXVivo Serum-Free Tumorsphere Media</a></li> <li><a href="/p/cell-culture/stemxvivo-serum-free-dendritic-cell-base-media_ccm003">StemXVivo Serum-Free Dendritic Cell Base Media</a></li> <li><a href="/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins">Cultrex<sup>™</sup> Basement Membrane Extracts and ECM Proteins</a></li> <li><a href="/p/stem-cell/human-methylcellulose-serum-free-enriched-media_hsc005sf">Human Methylcellulose Serum-Free Enriched</a> and <a href="/p/stem-cell/human-methylcellulose-serum-free-base-media_hsc002sf">Base Media</a></li> <li><a href="/p/stem-cell/human-methylcellulose-serum-free-enriched-media_hsc005sf">Serum-free methylcellulose media</a> for hematopoietic stem cell culture</li> <li><a href="/p/cell-culture/its-media-supplement-100x_ar013">ITS media supplement</a> for cell culture under low serum conditions</li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/serum-free-cell-culture.jpg" alt="adherent cells in serum-free culture" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Read more about developing serum-free cell culture workflows.</p> <p><a href="https://www.rndsystems.com/blog/what-makes-new-cultrex-ultimatrix-bme-matrix-stem-cell-and-organoid-cultures" target="_blank">Cultrex UltiMatrix BME – A Versatile Matrix for Organoid and Stem Cell Culture</a> at R&D Systems</p> <p><a href="https://resources.rndsystems.com/images/site/rnd-systems-isscr-2020-ipsc-surface-ps.pdf" target="_blank">Effects of Defined and Undefined Extracellular Matrices on Human iPSC Expansion and Differentiation</a> at R&D Systems</p> <p><a href="https://resources.rndsystems.com/pdfs/appnotes/rndsytems-an_optimizing-organoid_STRY0110888-final.pdf" target="_blank">Optimizing Organoid Culture Conditions</a> at R&D Systems</p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Animal-Free Cell Culture</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Animal-free media and their components are never exposed to potential contamination by animal products at any point during their manufacturing. In addition to its importance for robust culture processes, adopting animal-free materials also reduces the use of animals in reagent production. These materials support translational research by</p><ul><li>Eliminating risk of contamination with mammalian pathogens</li><li>Reducing manufacturing risk by reducing batch failure</li><li>Eliminating variability due to trace animal components</li><li><a href="/p/cell-culture/gmp-n-2-max-media-supplement-100x-animal-free_ar016">GMP N-2 MAX Media Supplement, animal-free</a></li><li><a href="https://www.tocris.com/product-type/gmp-ancillary-material-grade-small-molecules" target="_blank">GMP and Ancillary Material grade small molecules, animal-free</a></li><li><a href="/reagents/proteins/animal-free-proteins">Animal-free recombinant proteins</a></li><li><a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-component-free_ccm036">ExCellerate™ iPSC Expansion Medium, animal component-free</a></li><li><a href="/p/cell-culture/excellerate-human-t-cell-expansion-media-xeno-free_ccm030">ExCellerate™ Human T Cell Expansion Media, xeno-free</a></li><li><a href="/p/cell-culture/gmp-human-t-cell-media_ccm038-gmp">GMP T Cell Media</a></li><li><a href="/p/cell-culture/excellerate-human-nk-cell-expansion-media-animal-component-free_ccm037">ExCellerate Human NK Cell Expansion Media, animal component-free</a></li><li><a href="/p/cell-culture/excellerate-b-cell-media-xeno-free_ccm031">ExCellerate B Cell Media, xeno-free</a></li></ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/pluripotent-525x400.jpg" alt="pluripotent stem cells in animal-free culture" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Read our complete <a href="https://www.rndsystems.com/products/animal-free-statement">Animal-Free Statement</a> to learn about our dedicated labs, SOPs, QA protocols for deviations, and certification of BSE and TSE inactivation. The production of animal component-free reagents utilizes animal-free equipment and raw materials outside of our dedicated animal-free laboratories.</p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/cell-culture-reagents/excellerate" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/optimizing-organoid-app-note-bg.jpg" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/v2_Human-NK-Cell-Expasion_S2.png" alt="ExCellerate Cell Expansion Media bottles" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/cell-culture-reagents/excellerate" > <h3 class="card_title_htag"> <div><h3>ExCellerate Cell Expansion Media</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul><li>Serum-free formulations optimized for immune cell types and iPSCs</li><li>Generates high cell yields with minimal variability</li><li>Samples available - <a href="/reagents/cell-culture-reagents/excellerate#request-form">request yours now</a>!</li></ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/cell-culture-reagents/excellerate" > Create Consistent Cultures </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/proteins/animal-free-proteins" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/custom-protein-library-teaser-bg.jpg" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/custom-proteins-frontilmg-480x480.png" alt="animal-free GMP cytokine structure" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/proteins/animal-free-proteins" > <h3 class="card_title_htag"> <div><h3>Animal-Free Cytokines and Growth Factors</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul> <li>Preclinical and GMP grades</li> <li>All production lots tested against master lot to control for assay variability</li> <li>Same biological activity as our research grade proteins</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/proteins/animal-free-proteins" > Browse Our Animal-Free Proteins </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://www.regmednet.com/technology-digest-the-importance-of-serum-and-animal-component-free-media-for-stem-cell-based-therapeutics/" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/stem-cell-reserach-area-525x315.jpg" alt="PST - Stem Cell Reserach Area 525x315" loading="lazy" > </div> </a> <a class="card_title_link" href="https://www.regmednet.com/technology-digest-the-importance-of-serum-and-animal-component-free-media-for-stem-cell-based-therapeutics/" > <h3 class="card_title_htag"> <div><h3>The Importance of Serum- and Animal Component-Free Media </h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Read this article for a discussion covering:</p> <ul> <li>Cell culture media: getting it right from the start</li> <li>Advantages of using animal component-free media</li> <li>Where are we now with serum-free alternatives?</li> </ul> <p> </p></div> </div> <div class="card-bottom-link"> <div> <a href="https://www.regmednet.com/technology-digest-the-importance-of-serum-and-animal-component-free-media-for-stem-cell-based-therapeutics/" > Read Our Technology Digest Article at RegMedNet </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/small-molecules-and-peptides/cept-cocktail" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cept_cocktail_teaser_card.png" alt="Stem Cell Cluster" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/small-molecules-and-peptides/cept-cocktail" > <h3 class="card_title_htag"> <div>CEPT Cocktail for Improved Cell Survival</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Improve stem cell survival with our small molecule CEPT cocktail. A combination of 4 small molecules, <a href="/p/small-molecules-peptides/chroman-1_7163"><strong>C</strong>hroman 1</a>, <a href="/p/small-molecules-peptides/emricasan_7310"><strong>E</strong>mricasan</a>, <a href="/p/cell-culture/lyophilized-polyamine-supplement-x1000_7739">Lyophilized<strong> P</strong>olyamine Supplement</a> and <a href="/p/small-molecules-peptides/trans-isrib_5284"><strong>T</strong>rans-ISRIB</a>, the CEPT cocktail enhances the viability of stem cells during passaging, single cell cloning, gene-editing, cryopreservation, and differentiation and can be used in the manufacture of organoids for use in disease modeling or drug screening.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/small-molecules-and-peptides/cept-cocktail" > Learn More About CEPT Cocktail </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>RUO to Animal-Free to GMP</h2></div> <a href="/gmp-products/ruo-to-gmp" > <button type="button" class="btn btn-primary"> Streamline Your Transition to GMP </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h3>Specialty Cell Culture</h3></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Different lineages of cells require unique media compositions for the cells to proliferate, maintain viability, and retain their correct phenotypes and functions. The following pages provide a wider look into culture media components, differentiation kits, and characterization kits for these specialty cell culture applications. More can be learned from R&D Systems, a Bio-Techne brand, in the links below.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/research-areas/stem-cells" class="btn btn-primary" type="link"> Stem Cell Culture </a> </div> <div class="col-md-4"> <a href="/research-areas/organoids-3d-culture" class="btn btn-primary" type="link"> Organoid and 3D Cell Culture </a> </div> <div class="col-md-4"> <a href="/research-areas/immunology/immune-cell-selection-and-culture" class="btn btn-primary" type="link"> Immune Cell Culture </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/research-areas/neuroscience/neural-culture" class="btn btn-primary" type="link"> Neural Cell Culture </a> </div> <div class="col-md-4"> <a href="https://www.rndsystems.com/products/media-and-supplements-mesenchymal-stem-cells" class="btn btn-primary" type="link"> Mesenchymal Stem Cell Culture </a> </div> <div class="col-md-4"> <a href="https://www.rndsystems.com/products/cancer-stem-cell" class="btn btn-primary" type="link"> Cancer Stem Cell Culture </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Check for the Latest Promotions</h2></div> <a href="https://www.rndsystems.com/promotions#cell" > <button type="button" class="btn btn-primary"> Cell Culture Promotions </button> </a> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-additional-resources7" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Additional Resources</h5> </a> </div> <div class="collapse" id="expPara-additional-resources7" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p><strong>Website Resources</strong></p> <ul> <li><a href="/reagents/cell-culture-reagents/excellerate">ExCellerate Serum-Free and Animal-Free Cell Expansion Media</a></li> <li><a href="/reagents/cell-culture-reagents">Cell Culture Reagents</a></li> <li><a href="/research-areas/stem-cells">Stem Cells</a></li> <li><a href="/research-areas/organoids-3d-culture">Organoids and 3D Culture</a></li> <li><a href="/reagents/proteins">Recombinant Proteins</a></li> <li><a href="/reagents/proteins/liquid-cytokines-workflow">Liquid Cytokines</a></li> <li><a href="/reagents/small-molecules-and-peptides">Small Molecules & Peptides</a></li> <li><a href="/research-areas/cell-and-gene-therapy">Cell and Gene Therapy</a></li> <li><a href="/applications/bioprocessing">Bioprocessing</a></li> <li><a href="/research-areas/coronavirus">COVID-19</a></li> <li><a href="https://www.rndsystems.com/resources/cell-markers">Interactive Cell Markers Tool</a> at R&D Systems</li> </ul> <p><strong>Literature</strong></p> <ul> <li><a href="//resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-til-expansion-with-t-cell-media-v2.pdf" target="_blank">Enhance Your TIL Expansion with ExCellerate T Cell Media Application Note</a></li> <li><a href="/resources/literature/organoid-handbook" target="_self">The Organoid Culture Handbook</a></li> <li><a href="https://www.rndsystems.com/resources/ebook/evolution-model-systems-toxicology-drug-screening-and-disease-modeling" target="_blank">Evolution of Cell Culture Model Systems eBook</a> at R&D Systems</li> <li><a href="/resources/literature/t-cell-therapies-ebook">T Cell-Based Therapies eBook</a></li> <li><a href="https://www.rndsystems.com/protocol-types/stem-cell-protocols" target="_blank">Stem Cell Protocols</a> at R&D Systems</li> <li><a href="https://www.rndsystems.com/protocol-types/immunology">Immune Cell Protocols</a> at R&D Systems</li> <li><a href="/resources/literature/neural-cell-culturing-guide">Neural Cell Culturing Guide</a></li> <li><a href="https://www.rndsystems.com/protocol-types/neural-cell-culturing" target="_blank">Neural Cell Culturing Protocols</a> at R&D Systems</li> </ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-about-cell-culture-reagents13" role="button" aria-expanded="false" aria-controls="expPara"> <h5>About Cell Culture Reagents</h5> </a> </div> <div class="collapse" id="expPara-about-cell-culture-reagents13" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Cell culture requires understanding the nutrient and growth factor requirements of the cell population under study. This knowledge is valuable for the optimization of base media formulation and supplements including growth factors, cytokines, hormones, small molecules, and amino acids. Serum, such as fetal bovine serum (FBS), can supply unidentified factors which may be required for cell growth. The use of chemically defined media minimizes exposure of cultured cells to unidentified factors. Certified xeno-free, animal-free, or GMP grades of cell culture media are important for applications such as cell and gene therapy which involve human patient treatment. Well optimized cell culture media maintain the desired cell phenotype, robust proliferation, and high viability.</p> <p>Bio-Techne provides high quality cell culture reagents for research and cell manufacturing. We develop and manufacture cell culture and cryopreservation media, serum, defined media supplements, bioactive growth factors and small molecules, hydrogels, extracellular matrices, and basement membrane extracts for cell culture attachment.</p></div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div class="col-xl-9 col-lg-auto"> <p class="ml-xl-4 bt-px_groteskbold footer-tagline">Global Developer, Manufacturer, and Supplier of High-Quality Reagents, Analytical Instruments, and Precision Diagnostics.</p> </div> <div class="included-brands col"> <span class="font-weight-bold mr-3 bt-inter_regular">INCLUDES</span> <span class="mr-3 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