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<!DOCTYPE html> <html > <head> <meta charset="utf-8"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <meta content="width=device-width, initial-scale=1" name="viewport"> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs"> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="MG5MH6B4b8FGxTCGV/xU4zapA0bViNnwI2Li4xH8IeDbrZcTHccJOTBRC/gWGXV67Vq8iTbs9ZMSdluRtsoosg==" /> <meta name="citation_title" content="Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media" /> <meta name="citation_publication_date" content="2010/01/01" /> <meta name="citation_journal_title" content="The Pediatric infectious disease journal" /> <meta name="citation_author" content="Ravinder Kaur" /> <meta name="twitter:card" content="summary" /> <meta name="twitter:url" content="https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media" /> <meta name="twitter:title" content="Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media" /> <meta name="twitter:description" content="The 3 most commonly encountered bacteria in acute otitis media (AOM) are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus" /> <meta name="twitter:image" content="http://a.academia-assets.com/images/twitter-card.jpeg" /> <meta property="fb:app_id" content="2369844204" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media" /> <meta property="og:title" content="Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media" /> <meta property="og:image" content="http://a.academia-assets.com/images/open-graph-icons/fb-paper.gif" /> <meta property="og:description" content="The 3 most commonly encountered bacteria in acute otitis media (AOM) are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus" /> <meta property="article:author" content="https://independent.academia.edu/RavinderKaur87" /> <meta name="description" content="The 3 most commonly encountered bacteria in acute otitis media (AOM) are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus" /> <title>(PDF) Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media | Ravinder Kaur - Academia.edu</title> <link rel="canonical" href="https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '92477ec68c09d28ae4730a4143c926f074776319'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1732880194000); window.Aedu.timeDifference = new Date().getTime() - 1732880194000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"The 3 most commonly encountered bacteria in acute otitis media (AOM) are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus otitidis, another potential pathogen, has often been ignored. Tympanocentesis was performed in 97 children with AOM presenting with a bulging tympanic membrane (TM) producing 170 middle ear fluids (MEFs). S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis were isolated in 21%, 32%, 8%, and 0% of MEFs, respectively; no otopathogen was isolated in 29% of MEFs. In nasopharyngeal cultures at the time of AOM diagnosis, 34%, 36%, 17%, and 0% and in oropharyngeal cultures, 7%, 31%, 11%, and 0% grew S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis, respectively. No otopathogen was isolated in 23% of nasopharyngeal and 20% of oropharyngeal cultures. Multiplex polymerase chain reaction (PCR) was used to detect DNA of the 4...","author":[{"@context":"https://schema.org","@type":"Person","name":"Ravinder Kaur"}],"contributor":[],"dateCreated":"2022-06-07","dateModified":null,"datePublished":"2010-01-01","headline":"Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media","inLanguage":"en","keywords":["Pediatrics","Molecular Biology","Medicine","Humans","Female","Male","Bacteria","Polymerase Chain Reaction","Infant","multiplex PCR","Otitis Media","Bacterial infections","Nino","Child preschool","Acute Otitis Media","Nasopharynx","Paediatrics and reproductive medicine","multiplex polymerase chain reaction","Oropharynx"],"locationCreated":null,"publication":"The Pediatric infectious disease journal","publisher":{"@context":"https://schema.org","@type":"Organization","name":null},"image":null,"thumbnailUrl":null,"url":"https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media","sourceOrganization":[{"@context":"https://schema.org","@type":"EducationalOrganization","name":null}]}</script><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/single_work_page/loswp-102fa537001ba4d8dcd921ad9bd56c474abc201906ea4843e7e7efe9dfbf561d.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/body-8d679e925718b5e8e4b18e9a4fab37f7eaa99e43386459376559080ac8f2856a.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/button-3cea6e0ad4715ed965c49bfb15dedfc632787b32ff6d8c3a474182b231146ab7.css" /><link rel="stylesheet" media="all" 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Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus otitidis, another potential pathogen, has often been ignored. Tympanocentesis was performed in 97 children with AOM presenting with a bulging tympanic membrane (TM) producing 170 middle ear fluids (MEFs). S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis were isolated in 21%, 32%, 8%, and 0% of MEFs, respectively; no otopathogen was isolated in 29% of MEFs. In nasopharyngeal cultures at the time of AOM diagnosis, 34%, 36%, 17%, and 0% and in oropharyngeal cultures, 7%, 31%, 11%, and 0% grew S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis, respectively. No otopathogen was isolated in 23% of nasopharyngeal and 20% of oropharyngeal cultures. Multiplex polymerase chain reaction (PCR) was used to detect DNA of the 4...","publication_date":"2010,,","publication_name":"The Pediatric infectious disease journal"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media","broadcastable":false,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [67299631]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":87162382,"attachmentType":"pdf"}"><img alt="First page of “Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/87162382/mini_magick20220607-11392-113js2u.png?1654634220" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="67299631" href="https://independent.academia.edu/RavinderKaur87"><img alt="Profile image of Ravinder Kaur" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Ravinder Kaur</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2010, The Pediatric infectious disease journal</p></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">The 3 most commonly encountered bacteria in acute otitis media (AOM) are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus otitidis, another potential pathogen, has often been ignored. Tympanocentesis was performed in 97 children with AOM presenting with a bulging tympanic membrane (TM) producing 170 middle ear fluids (MEFs). S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis were isolated in 21%, 32%, 8%, and 0% of MEFs, respectively; no otopathogen was isolated in 29% of MEFs. In nasopharyngeal cultures at the time of AOM diagnosis, 34%, 36%, 17%, and 0% and in oropharyngeal cultures, 7%, 31%, 11%, and 0% grew S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis, respectively. No otopathogen was isolated in 23% of nasopharyngeal and 20% of oropharyngeal cultures. Multiplex polymerase chain reaction (PCR) was used to detect DNA of the 4...</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":87162382,"attachmentType":"pdf","workUrl":"https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":87162382,"attachmentType":"pdf","workUrl":"https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download 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href="https://www.academia.edu/67578071/Identification_of_Alloiococcus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_Children_With_Otitis_Media_With_Effusion">Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="19858732" href="https://independent.academia.edu/hamedgoodarzi">hamed goodarzi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Jundishapur Journal of Microbiology, 2015</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion","attachmentId":78343484,"attachmentType":"pdf","work_url":"https://www.academia.edu/67578071/Identification_of_Alloiococcus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_Children_With_Otitis_Media_With_Effusion","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/67578071/Identification_of_Alloiococcus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_Children_With_Otitis_Media_With_Effusion"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="889153" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/889153/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_">Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in …</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45029" href="https://tums.academia.edu/babaksaedi">Babak Saedi</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="524053" href="https://tums.academia.edu/ERazmpa">Ebrahim Razmpa</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in …","attachmentId":51179989,"attachmentType":"pdf","work_url":"https://www.academia.edu/889153/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/889153/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="28383181" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/28383181/Frequency_of_i_Alloicoccus_otitidis_i_i_Streptococcus_pneumoniae_i_i_Moraxella_catarrhalis_i_and_i_Haemophilus_influenzae_i_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients">Frequency of< i> Alloicoccus otitidis</i>,< i> Streptococcus pneumoniae</i>,< i> Moraxella catarrhalis</i> and< i> Haemophilus influenzae</i> in children with otitis media with effusion (OME) in Iranian patients</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45029" href="https://tums.academia.edu/babaksaedi">Babak Saedi</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="524053" href="https://tums.academia.edu/ERazmpa">Ebrahim Razmpa</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="53213336" href="https://yasujmedicalsciences.academia.edu/seyedsajjadkhoramrooz">seyed sajjad khoramrooz</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2012</p><p class="ds-related-work--abstract ds2-5-body-sm">OBJECTIVE: To determine the presence of common bacterial agents of otitis media with effusion (OME), together with investigation these agent in the adenoid tissue and antimicrobial susceptibility pattern of isolated bacteria in Iranian children with OME. METHODS: Polymerase chain reaction (PCR) and bacterial culture methods were used for detection and isolation of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in 63 middle ear fluid samples and 48 adenoid ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Frequency of\u003c i\u003e Alloicoccus otitidis\u003c/i\u003e,\u003c i\u003e Streptococcus pneumoniae\u003c/i\u003e,\u003c i\u003e Moraxella catarrhalis\u003c/i\u003e and\u003c i\u003e Haemophilus influenzae\u003c/i\u003e in children with otitis media with effusion (OME) in Iranian patients","attachmentId":48818707,"attachmentType":"pdf","work_url":"https://www.academia.edu/28383181/Frequency_of_i_Alloicoccus_otitidis_i_i_Streptococcus_pneumoniae_i_i_Moraxella_catarrhalis_i_and_i_Haemophilus_influenzae_i_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/28383181/Frequency_of_i_Alloicoccus_otitidis_i_i_Streptococcus_pneumoniae_i_i_Moraxella_catarrhalis_i_and_i_Haemophilus_influenzae_i_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="28383183" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/28383183/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients">Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in Iranian patients</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45029" href="https://tums.academia.edu/babaksaedi">Babak Saedi</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="53213336" href="https://yasujmedicalsciences.academia.edu/seyedsajjadkhoramrooz">seyed sajjad khoramrooz</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in Iranian patients","attachmentId":48718857,"attachmentType":"pdf","work_url":"https://www.academia.edu/28383183/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/28383183/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="17519657" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/17519657/Identification_and_characterization_of_the_bacterial_etiology_of_clinically_problematic_acute_otitis_media_after_tympanocentesis_or_spontaneous_otorrhea_in_German_children">Identification and characterization of the bacterial etiology of clinically problematic acute otitis media after tympanocentesis or spontaneous otorrhea in German children</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37287925" href="https://gsk.academia.edu/CinziaMarano">Cinzia Marano</a></div><p class="ds-related-work--metadata ds2-5-body-xs">BMC infectious diseases, 2012</p><p class="ds-related-work--abstract ds2-5-body-sm">Acute Otitis Media (AOM) is an important and common disease of childhood. Bacteria isolated from cases of clinically problematic AOM in German children were identified and characterized. In a prospective non-interventional study in German children between 3 months and less than 60 months of age with Ear, Nose and Throat Specialist -confirmed AOM, middle ear fluid was obtained by tympanocentesis (when clinically indicated) or by careful sampling of otorrhea through/at an existing perforation. In 100 children with severe AOM, Haemophilus influenzae was identified in 21% (18/21, 85.7% were non-typeable [NTHi]), Streptococcus pneumoniae in 10%, S. pyogenes in 13% and Moraxella catarrhalis in 1%. H. influenzae was the most frequently identified pathogen in children from 12 months of age. H. influenzae and S. pneumoniae were equally prevalent in children aged 3-11 months, but S. pyogenes was most frequently isolated in this age group. NTHi AOM disease appeared prevalent in all ages. NTHi,...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Identification and characterization of the bacterial etiology of clinically problematic acute otitis media after tympanocentesis or spontaneous otorrhea in German children","attachmentId":42252026,"attachmentType":"pdf","work_url":"https://www.academia.edu/17519657/Identification_and_characterization_of_the_bacterial_etiology_of_clinically_problematic_acute_otitis_media_after_tympanocentesis_or_spontaneous_otorrhea_in_German_children","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17519657/Identification_and_characterization_of_the_bacterial_etiology_of_clinically_problematic_acute_otitis_media_after_tympanocentesis_or_spontaneous_otorrhea_in_German_children"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="10942648" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/10942648/Concurrent_Assay_for_Four_Bacterial_Species_Including_Alloiococcus_Otitidis_in_Middle_Ear_Nasopharynx_and_Tonsils_of_Children_with_Otitis_Media_with_Effusion_A_Preliminary_Report">Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="26524495" href="https://independent.academia.edu/EmineAyd%C4%B1n4">Emine Aydın</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinical and Experimental Otorhinolaryngology, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report","attachmentId":47009831,"attachmentType":"pdf","work_url":"https://www.academia.edu/10942648/Concurrent_Assay_for_Four_Bacterial_Species_Including_Alloiococcus_Otitidis_in_Middle_Ear_Nasopharynx_and_Tonsils_of_Children_with_Otitis_Media_with_Effusion_A_Preliminary_Report","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/10942648/Concurrent_Assay_for_Four_Bacterial_Species_Including_Alloiococcus_Otitidis_in_Middle_Ear_Nasopharynx_and_Tonsils_of_Children_with_Otitis_Media_with_Effusion_A_Preliminary_Report"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="24572931" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/24572931/Two_step_PCR_based_assay_for_identification_of_bacterial_etiology_of_otitis_media_with_effusion_in_infected_Lebanese_children">Two-step PCR-based assay for identification of bacterial etiology of otitis media with effusion in infected Lebanese children</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="47367128" href="https://independent.academia.edu/FayadMichel">Michel Fayad</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of clinical microbiology, 1998</p><p class="ds-related-work--abstract ds2-5-body-sm">We developed and evaluated a two-step PCR-based assay with universal primers and genus- or species-specific primers for the detection of the most prevalent bacterial etiologies of otitis media with effusion (OME) in children from Lebanese hospitals. These etiologies included Haemophilus, Streptococcus, and Moraxella (Branhamella) catarrhalis, which were detected in middle-ear effusion (MEE) samples taken from children with OME. A total of 47 MEE samples were aspirated from 36 patients during insertion of a tympanostomy tube performed particularly for OME. The duration of effusion in all patients was &gt; or =2 months. DNA was extracted from MEE samples, and PCR was initially done with DNA extracts by using the universal primers RW01 and DG74, which flank an approximately 370-bp fragment found in the 16S rRNA gene of all bacterial species. For the identification of specific bacteria, we used in three separate reaction mixtures the following genus- or species-specific primers: (i) a H...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Two-step PCR-based assay for identification of bacterial etiology of otitis media with effusion in infected Lebanese children","attachmentId":44901779,"attachmentType":"pdf","work_url":"https://www.academia.edu/24572931/Two_step_PCR_based_assay_for_identification_of_bacterial_etiology_of_otitis_media_with_effusion_in_infected_Lebanese_children","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/24572931/Two_step_PCR_based_assay_for_identification_of_bacterial_etiology_of_otitis_media_with_effusion_in_infected_Lebanese_children"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="23308796" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/23308796/Lack_of_Chlamydophila_pneumoniae_and_predominance_of_Alloiococcus_otitidis_in_middle_ear_fluids_of_children_with_otitis_media_with_effusion">Lack of Chlamydophila pneumoniae and predominance of Alloiococcus otitidis in middle ear fluids of children with otitis media with effusion</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45077379" href="https://independent.academia.edu/EnderInci">Ender Inci</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Lack of Chlamydophila pneumoniae and predominance of Alloiococcus otitidis in middle ear fluids of children with otitis media with effusion","attachmentId":43771133,"attachmentType":"pdf","work_url":"https://www.academia.edu/23308796/Lack_of_Chlamydophila_pneumoniae_and_predominance_of_Alloiococcus_otitidis_in_middle_ear_fluids_of_children_with_otitis_media_with_effusion","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/23308796/Lack_of_Chlamydophila_pneumoniae_and_predominance_of_Alloiococcus_otitidis_in_middle_ear_fluids_of_children_with_otitis_media_with_effusion"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="48896723" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/48896723/Comparison_of_Culture_and_PCR_Methods_in_Detection_of_Haemophilus_influenzae_Streptococcus_pneumoniae_and_Moraxella_catarrhalis_in_Children_with_Otitis_Media_with_Effusion">Comparison of Culture and PCR Methods in Detection of Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis in Children with Otitis Media with Effusion</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="192839025" href="https://independent.academia.edu/abdullahabdullah645">abdullah abdullah</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Turkiye Klinikleri Journal of Medical Sciences</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Comparison of Culture and PCR Methods in Detection of Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis in Children with Otitis Media with Effusion","attachmentId":67303218,"attachmentType":"pdf","work_url":"https://www.academia.edu/48896723/Comparison_of_Culture_and_PCR_Methods_in_Detection_of_Haemophilus_influenzae_Streptococcus_pneumoniae_and_Moraxella_catarrhalis_in_Children_with_Otitis_Media_with_Effusion","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/48896723/Comparison_of_Culture_and_PCR_Methods_in_Detection_of_Haemophilus_influenzae_Streptococcus_pneumoniae_and_Moraxella_catarrhalis_in_Children_with_Otitis_Media_with_Effusion"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="25450483" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/25450483/Microbiology_of_acute_otitis_media_and_therapeutic_consequences">Microbiology of acute otitis media and therapeutic consequences</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32529483" href="https://independent.academia.edu/GunnarKahlmeter">Gunnar Kahlmeter</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International journal of pediatric otorhinolaryngology, 1995</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Microbiology of acute otitis media and therapeutic consequences","attachmentId":45768268,"attachmentType":"pdf","work_url":"https://www.academia.edu/25450483/Microbiology_of_acute_otitis_media_and_therapeutic_consequences","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link 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You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="33522874" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/33522874/Polymerase_chain_reaction_bacteriologic_detection_and_antibiogram_of_bacteria_isolated_from_otitis_media_with_effusion_in_children_shiraz_iran">Polymerase chain reaction, bacteriologic detection and antibiogram of bacteria isolated from otitis media with effusion in children, shiraz, iran</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="1847851" 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class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33832445" href="https://menzies.academia.edu/KimHare">Kim Hare</a></div><p class="ds-related-work--metadata ds2-5-body-xs">BMC Ear, Nose and Throat Disorders, 2013</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Dominance of Haemophilus influenzae in ear discharge from Indigenous Australian children with acute otitis media with tympanic membrane perforation","attachmentId":47229043,"attachmentType":"pdf","work_url":"https://www.academia.edu/26967112/Dominance_of_Haemophilus_influenzae_in_ear_discharge_from_Indigenous_Australian_children_with_acute_otitis_media_with_tympanic_membrane_perforation","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" 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href="https://www.academia.edu/47950062/Quantitative_PCR_of_ear_discharge_from_Indigenous_Australian_children_with_acute_otitis_media_with_perforation_supports_a_role_for_Alloiococcus_otitidis_as_a_secondary_pathogen">Quantitative PCR of ear discharge from Indigenous Australian children with acute otitis media with perforation supports a role for Alloiococcus otitidis as a secondary pathogen</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="147569031" href="https://independent.academia.edu/MichaelBinks">Michael Binks</a></div><p class="ds-related-work--metadata ds2-5-body-xs">BMC Ear, Nose and Throat Disorders, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Quantitative PCR of ear discharge from Indigenous Australian children with acute otitis media with perforation supports a role for Alloiococcus otitidis as a secondary pathogen","attachmentId":66816021,"attachmentType":"pdf","work_url":"https://www.academia.edu/47950062/Quantitative_PCR_of_ear_discharge_from_Indigenous_Australian_children_with_acute_otitis_media_with_perforation_supports_a_role_for_Alloiococcus_otitidis_as_a_secondary_pathogen","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/47950062/Quantitative_PCR_of_ear_discharge_from_Indigenous_Australian_children_with_acute_otitis_media_with_perforation_supports_a_role_for_Alloiococcus_otitidis_as_a_secondary_pathogen"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="3" data-entity-id="88241422" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/88241422/Characterization_of_Alloiococcus_otitidis_strains_isolated_from_children_with_otitis_media_with_effusion_by_Pulsed_Field_Gel_Electrophoresis">Characterization of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel Electrophoresis</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="524053" href="https://tums.academia.edu/ERazmpa">Ebrahim Razmpa</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Pediatric Otorhinolaryngology, 2012</p><div class="ds-related-work--ctas"><button 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