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class="user-info-component-wrapper"><div class="user-summary-cta-container"><div class="user-summary-container"><div class="social-profile-avatar-container"><img class="profile-avatar u-positionAbsolute" alt="Jangwook Lee" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/132857751/42561155/34110660/s200_jangwook.lee.jpg" /></div><div class="title-container"><h1 class="ds2-5-heading-sans-serif-sm">Jangwook Lee</h1><div class="affiliations-container fake-truncate js-profile-affiliations"></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="Jangwook" data-follow-user-id="132857751" data-follow-user-source="profile_button" 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href="https://www.academia.edu/110578274/Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size"><img alt="Research paper thumbnail of Two distinct cellular pathways leading to endothelial cell cytotoxicity by silica nanoparticle size" class="work-thumbnail" src="https://attachments.academia-assets.com/108352132/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/110578274/Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size">Two distinct cellular pathways leading to endothelial cell cytotoxicity by silica nanoparticle size</a></div><div class="wp-workCard_item"><span>Journal of Nanobiotechnology</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for the targeted delivery of therapeutic agents. Safety concerns about the biomedical and clinical applications of SiNPs have been raised, necessitating analysis of the effects of their intrinsic properties, such as sizes, shapes, and surface physicochemical characteristics, on human health to minimize risk in biomedical applications. In particular, SiNP size-associated toxicological effects, and the underlying molecular mechanisms in the vascular endothelium remain unclear. This study aimed to elucidate the detailed mechanisms underlying the cellular response to exposure to trace amounts of SiNPs and to determine applicable size criteria for biomedical application. Methods: To clarify whether these SiNP-mediated cytotoxicity due to induction of apoptosis or necrosis, human ECs were treated with SiNPs of four different non-overlapping sizes under low serum-containing condition, stained with annexin V and propidium iodide (PI), and subjected to flow cytometric analysis (FACS). Two types of cell death mechanisms were assessed in terms of production of reactive oxygen species (ROS), endoplasmic reticulum (ER) stress induction, and autophagy activity. Results: Spherical SiNPs had a diameter of 21.8 nm; this was further increased to 31.4, 42.9, and 56.7 nm. Hence, we investigated these effects in human endothelial cells (ECs) treated with these nanoparticles under overlap-or agglomerate-free conditions. The 20-nm SiNPs, but not SiNPs of other sizes, significantly induced apoptosis and necrosis. Surprisingly, the two types of cell death occurred independently and through different mechanisms. Apoptotic cell death resulted from ROS-mediated ER stress. Furthermore, autophagy-mediated necrotic cell death was induced through the PI3K/AKT/eNOS signaling axis. Together, the present results indicate that SiNPs within a diameter of < 20-nm pose greater risks to cells in terms of cytotoxic effects.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="af1840bb7751995e503f826209485569" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":108352132,"asset_id":110578274,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/108352132/download_file?st=MTczNDU1OTUyOCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="110578274"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="110578274"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 110578274; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=110578274]").text(description); $(".js-view-count[data-work-id=110578274]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 110578274; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='110578274']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 110578274, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "af1840bb7751995e503f826209485569" } } $('.js-work-strip[data-work-id=110578274]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":110578274,"title":"Two distinct cellular pathways leading to endothelial cell cytotoxicity by silica nanoparticle size","translated_title":"","metadata":{"publisher":"Springer Science and Business Media LLC","grobid_abstract":"Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for the targeted delivery of therapeutic agents. Safety concerns about the biomedical and clinical applications of SiNPs have been raised, necessitating analysis of the effects of their intrinsic properties, such as sizes, shapes, and surface physicochemical characteristics, on human health to minimize risk in biomedical applications. In particular, SiNP size-associated toxicological effects, and the underlying molecular mechanisms in the vascular endothelium remain unclear. This study aimed to elucidate the detailed mechanisms underlying the cellular response to exposure to trace amounts of SiNPs and to determine applicable size criteria for biomedical application. Methods: To clarify whether these SiNP-mediated cytotoxicity due to induction of apoptosis or necrosis, human ECs were treated with SiNPs of four different non-overlapping sizes under low serum-containing condition, stained with annexin V and propidium iodide (PI), and subjected to flow cytometric analysis (FACS). Two types of cell death mechanisms were assessed in terms of production of reactive oxygen species (ROS), endoplasmic reticulum (ER) stress induction, and autophagy activity. Results: Spherical SiNPs had a diameter of 21.8 nm; this was further increased to 31.4, 42.9, and 56.7 nm. Hence, we investigated these effects in human endothelial cells (ECs) treated with these nanoparticles under overlap-or agglomerate-free conditions. The 20-nm SiNPs, but not SiNPs of other sizes, significantly induced apoptosis and necrosis. Surprisingly, the two types of cell death occurred independently and through different mechanisms. Apoptotic cell death resulted from ROS-mediated ER stress. Furthermore, autophagy-mediated necrotic cell death was induced through the PI3K/AKT/eNOS signaling axis. Together, the present results indicate that SiNPs within a diameter of \u003c 20-nm pose greater risks to cells in terms of cytotoxic effects.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"Journal of Nanobiotechnology","grobid_abstract_attachment_id":108352132},"translated_abstract":null,"internal_url":"https://www.academia.edu/110578274/Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size","translated_internal_url":"","created_at":"2023-12-04T15:10:54.059-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":108352132,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/108352132/thumbnails/1.jpg","file_name":"s12951-019-0456-4.pdf","download_url":"https://www.academia.edu/attachments/108352132/download_file?st=MTczNDU1OTUyOCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Two_distinct_cellular_pathways_leading_t.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/108352132/s12951-019-0456-4-libre.pdf?1701732329=\u0026response-content-disposition=attachment%3B+filename%3DTwo_distinct_cellular_pathways_leading_t.pdf\u0026Expires=1734563128\u0026Signature=QcKKxegpgeMpmAVqMeZETlWx4HgATuTd-MrNgnApSzkG-shYAt~S4WHu5Wiohbcl1YgL~mR9iHf0SwoIbni0naga~QQtbcreUNmmO2JuvOejEKFIyMQNgkFq0Qd5gNfdUrwKKTJ3savss0o5Q6eKJ1Us4Wy68WUP9AUtgHHA74cDj51QsldcQWxvX4knwFvvUw0u9RW7W0yRB4G9OKLRHtSBYsAkY9wWHONtBfZaUQfe0CWWy6VHj7yxi45vY2IuGmW6~eUH8prndM4kehDgiC-NQFP0fsI-T4gPTcxLDTz0Ka5BdhGFD2WnCS4~l9pY2W42E9qXc3MY~hTb--ovMw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size","translated_slug":"","page_count":14,"language":"en","content_type":"Work","summary":"Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for the targeted delivery of therapeutic agents. Safety concerns about the biomedical and clinical applications of SiNPs have been raised, necessitating analysis of the effects of their intrinsic properties, such as sizes, shapes, and surface physicochemical characteristics, on human health to minimize risk in biomedical applications. In particular, SiNP size-associated toxicological effects, and the underlying molecular mechanisms in the vascular endothelium remain unclear. This study aimed to elucidate the detailed mechanisms underlying the cellular response to exposure to trace amounts of SiNPs and to determine applicable size criteria for biomedical application. Methods: To clarify whether these SiNP-mediated cytotoxicity due to induction of apoptosis or necrosis, human ECs were treated with SiNPs of four different non-overlapping sizes under low serum-containing condition, stained with annexin V and propidium iodide (PI), and subjected to flow cytometric analysis (FACS). Two types of cell death mechanisms were assessed in terms of production of reactive oxygen species (ROS), endoplasmic reticulum (ER) stress induction, and autophagy activity. Results: Spherical SiNPs had a diameter of 21.8 nm; this was further increased to 31.4, 42.9, and 56.7 nm. Hence, we investigated these effects in human endothelial cells (ECs) treated with these nanoparticles under overlap-or agglomerate-free conditions. The 20-nm SiNPs, but not SiNPs of other sizes, significantly induced apoptosis and necrosis. Surprisingly, the two types of cell death occurred independently and through different mechanisms. Apoptotic cell death resulted from ROS-mediated ER stress. Furthermore, autophagy-mediated necrotic cell death was induced through the PI3K/AKT/eNOS signaling axis. Together, the present results indicate that SiNPs within a diameter of \u003c 20-nm pose greater risks to cells in terms of cytotoxic effects.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":108352132,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/108352132/thumbnails/1.jpg","file_name":"s12951-019-0456-4.pdf","download_url":"https://www.academia.edu/attachments/108352132/download_file?st=MTczNDU1OTUyOCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Two_distinct_cellular_pathways_leading_t.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/108352132/s12951-019-0456-4-libre.pdf?1701732329=\u0026response-content-disposition=attachment%3B+filename%3DTwo_distinct_cellular_pathways_leading_t.pdf\u0026Expires=1734563128\u0026Signature=QcKKxegpgeMpmAVqMeZETlWx4HgATuTd-MrNgnApSzkG-shYAt~S4WHu5Wiohbcl1YgL~mR9iHf0SwoIbni0naga~QQtbcreUNmmO2JuvOejEKFIyMQNgkFq0Qd5gNfdUrwKKTJ3savss0o5Q6eKJ1Us4Wy68WUP9AUtgHHA74cDj51QsldcQWxvX4knwFvvUw0u9RW7W0yRB4G9OKLRHtSBYsAkY9wWHONtBfZaUQfe0CWWy6VHj7yxi45vY2IuGmW6~eUH8prndM4kehDgiC-NQFP0fsI-T4gPTcxLDTz0Ka5BdhGFD2WnCS4~l9pY2W42E9qXc3MY~hTb--ovMw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology"},{"id":7835,"name":"Nanobiotechnology","url":"https://www.academia.edu/Documents/in/Nanobiotechnology"},{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology"},{"id":17923,"name":"Autophagy","url":"https://www.academia.edu/Documents/in/Autophagy"},{"id":22050,"name":"Cytotoxicity","url":"https://www.academia.edu/Documents/in/Cytotoxicity"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":117915,"name":"Unfolded Protein Response","url":"https://www.academia.edu/Documents/in/Unfolded_Protein_Response"},{"id":122187,"name":"Endoplasmic Reticulum","url":"https://www.academia.edu/Documents/in/Endoplasmic_Reticulum"},{"id":175490,"name":"Programmed cell death","url":"https://www.academia.edu/Documents/in/Programmed_cell_death"},{"id":1335154,"name":"Propidium Iodide","url":"https://www.academia.edu/Documents/in/Propidium_Iodide"},{"id":3101476,"name":"Annexin","url":"https://www.academia.edu/Documents/in/Annexin"}],"urls":[{"id":36452227,"url":"http://link.springer.com/content/pdf/10.1186/s12951-019-0456-4.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="93323682"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/93323682/A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms"><img alt="Research paper thumbnail of A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms" class="work-thumbnail" src="https://attachments.academia-assets.com/96092610/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/93323682/A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms">A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms</a></div><div class="wp-workCard_item"><span>International Journal of Nanomedicine</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: The size of nanoparticles is considered to influence their toxicity, as smallersized ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: The size of nanoparticles is considered to influence their toxicity, as smallersized nanoparticles should more easily penetrate the cell and exert toxic effects. However, conflicting results and unstandardized methodology have resulted in controversy of these size-dependent effects. Here, we introduce a unique approach to study such size-dependent effects of nanoparticles and present evidence that reliably supports this general assumption along with elucidation of the underlying cytotoxic mechanism. Methods: We prepared and physically characterized size-controlled (20-50 nm) monodispersed silica nanoparticles (SNPs) in aqueous suspensions. Then, a variety of biochemical assessments are used for evaluating the cytotoxic mechanisms. Results: SNP treatment in three cell lines decreased cell viability and migration ability, while ROS production increased in dose-and size-dependent manners, with SNPs <30 nm showing the greatest effects. 30-and 40-nm SNPs were observed similar to these biological activities of 20-and 50-nm, respectively. Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC 50 values (75.2 and 175.2 渭g/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs. Conclusion: Our results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b0ed2afeda5321698308f2855aa81125" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96092610,"asset_id":93323682,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96092610/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="93323682"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="93323682"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 93323682; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=93323682]").text(description); $(".js-view-count[data-work-id=93323682]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 93323682; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='93323682']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 93323682, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b0ed2afeda5321698308f2855aa81125" } } $('.js-work-strip[data-work-id=93323682]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":93323682,"title":"A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms","translated_title":"","metadata":{"publisher":"Informa UK Limited","grobid_abstract":"Background: The size of nanoparticles is considered to influence their toxicity, as smallersized nanoparticles should more easily penetrate the cell and exert toxic effects. However, conflicting results and unstandardized methodology have resulted in controversy of these size-dependent effects. Here, we introduce a unique approach to study such size-dependent effects of nanoparticles and present evidence that reliably supports this general assumption along with elucidation of the underlying cytotoxic mechanism. Methods: We prepared and physically characterized size-controlled (20-50 nm) monodispersed silica nanoparticles (SNPs) in aqueous suspensions. Then, a variety of biochemical assessments are used for evaluating the cytotoxic mechanisms. Results: SNP treatment in three cell lines decreased cell viability and migration ability, while ROS production increased in dose-and size-dependent manners, with SNPs \u003c30 nm showing the greatest effects. 30-and 40-nm SNPs were observed similar to these biological activities of 20-and 50-nm, respectively. Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC 50 values (75.2 and 175.2 渭g/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs. Conclusion: Our results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"International Journal of Nanomedicine","grobid_abstract_attachment_id":96092610},"translated_abstract":null,"internal_url":"https://www.academia.edu/93323682/A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms","translated_internal_url":"","created_at":"2022-12-20T03:59:13.835-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":96092610,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96092610/thumbnails/1.jpg","file_name":"getfile.pdf","download_url":"https://www.academia.edu/attachments/96092610/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_reliable_approach_for_assessing_size_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96092610/getfile-libre.pdf?1671540993=\u0026response-content-disposition=attachment%3B+filename%3DA_reliable_approach_for_assessing_size_d.pdf\u0026Expires=1734563128\u0026Signature=Mbe53J~ucad1r0cevCYCPawDmZE1DV5yXqsdSI3aSLoAj9GemOSTizVl-JV7Jj0qngF7ChxVaMO0HpAhNUSlFXh9b1iLzgvjrNNAnUQ~fYZMc2fxfDFvA8jeeXZgCz10kXM1Wh0-PXPcHvAgPVyjfXoWNMcSO62OSqbTQBDi-bdq3yrT4i4xlL6vy2qeuBSDoM~PLzBHmTyzXWlV43Bb4E008sdiVqckzljHvVncbJBJDE3fzWR0o5TEXEyXdcfuLK~y5I7Pzh43vcpcW1qOZYEk6a~zk9UFQ2b5-HGzBsSDYwviUng8ddzhsWv6X-bchpCrHXoxwqtnXfu8xyjJjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms","translated_slug":"","page_count":13,"language":"en","content_type":"Work","summary":"Background: The size of nanoparticles is considered to influence their toxicity, as smallersized nanoparticles should more easily penetrate the cell and exert toxic effects. However, conflicting results and unstandardized methodology have resulted in controversy of these size-dependent effects. Here, we introduce a unique approach to study such size-dependent effects of nanoparticles and present evidence that reliably supports this general assumption along with elucidation of the underlying cytotoxic mechanism. Methods: We prepared and physically characterized size-controlled (20-50 nm) monodispersed silica nanoparticles (SNPs) in aqueous suspensions. Then, a variety of biochemical assessments are used for evaluating the cytotoxic mechanisms. Results: SNP treatment in three cell lines decreased cell viability and migration ability, while ROS production increased in dose-and size-dependent manners, with SNPs \u003c30 nm showing the greatest effects. 30-and 40-nm SNPs were observed similar to these biological activities of 20-and 50-nm, respectively. Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC 50 values (75.2 and 175.2 渭g/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs. Conclusion: Our results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":96092610,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96092610/thumbnails/1.jpg","file_name":"getfile.pdf","download_url":"https://www.academia.edu/attachments/96092610/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_reliable_approach_for_assessing_size_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96092610/getfile-libre.pdf?1671540993=\u0026response-content-disposition=attachment%3B+filename%3DA_reliable_approach_for_assessing_size_d.pdf\u0026Expires=1734563129\u0026Signature=OhVrJcyxqgc0xGfhBEF~9hNsypr9wlwD6dKmjcbAfnlIMWeL5M-SPQjdAAKTmenpHcwEW~f9uiPWEPcIB7Momn8-6549TSBAoQjqQzK-9t73fTlnbBFoBO2udGMBVcB27uVLUKYuu~46-fZTmUTPIXjYY1JrCv3bpQ7yNXdpDy5HZTdZlmzAKbLXPL5anPymzYeJs-MRgSFJa8b1s3I4yjm~ETxxm5aUXytfKdwlKYqP1pwtWA5NEmF0ejSAYoZz4sF7OeRNZ6iFufDeHY3--mU4jlKBZc7HjZ5U9EYdSsXNz7hIy0qotJbX3mMpCrVTZSmRKG5DwMQQSyGqMycEjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":96092611,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96092611/thumbnails/1.jpg","file_name":"getfile.pdf","download_url":"https://www.academia.edu/attachments/96092611/download_file","bulk_download_file_name":"A_reliable_approach_for_assessing_size_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96092611/getfile-libre.pdf?1671540988=\u0026response-content-disposition=attachment%3B+filename%3DA_reliable_approach_for_assessing_size_d.pdf\u0026Expires=1734563129\u0026Signature=C6wwjxVNdqDY91AAuCaYGDQXPwREujtz-yUYFyhdoTprUHqiSrpLotbmNd8hQtCxhBMK583-TrubJ4AiyJyed-~BHSYVt~ZKyb3hwRmicDCS2Iduk3rRD9PqgpnjqFSej8~7Y70yzF7skb8m2Dg6NmSmo4ZNOfZ8SLNACNCcN-~MFgvKCEdH0dmQF9QKNvR5ClL3nKSNR1Vd65MoHsxmQEgeBaQ12pYILiv4ALusPKAyoLUsDGBCfMyHObqPAofJgVof5BvpbgIKcX-t2BXxAAMsow3QdhBjGxEhG1MVRcP--VXffOKtNtTtJliJDp2XX569~6BVWI7Jt7Xazk7BgQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":11972,"name":"Nanomedicine","url":"https://www.academia.edu/Documents/in/Nanomedicine"},{"id":17733,"name":"Nanotechnology","url":"https://www.academia.edu/Documents/in/Nanotechnology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":51613,"name":"Internalization","url":"https://www.academia.edu/Documents/in/Internalization"},{"id":90156,"name":"Endocytosis","url":"https://www.academia.edu/Documents/in/Endocytosis"},{"id":1335152,"name":"Viability assay","url":"https://www.academia.edu/Documents/in/Viability_assay"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":27247258,"url":"https://www.dovepress.com/getfile.php?fileID=52672"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320694"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320694/Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles"><img alt="Research paper thumbnail of Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320694/Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles">Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles</a></div><div class="wp-workCard_item"><span>Current Pharmaceutical Design</span><span>, Mar 1, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide e...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320694"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320694"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320694; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320694]").text(description); $(".js-view-count[data-work-id=60320694]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320694; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320694']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320694, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320694]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320694,"title":"Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles","translated_title":"","metadata":{"abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.","publication_date":{"day":1,"month":3,"year":2015,"errors":{}},"publication_name":"Current Pharmaceutical Design"},"translated_abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.","internal_url":"https://www.academia.edu/60320694/Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles","translated_internal_url":"","created_at":"2021-10-29T08:02:16.270-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":39857,"name":"Molecular Imaging","url":"https://www.academia.edu/Documents/in/Molecular_Imaging"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":2349258,"name":"Serum albumin","url":"https://www.academia.edu/Documents/in/Serum_albumin"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":13799184,"url":"http://pubmed.cn/25732551"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320691"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320691/In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes"><img alt="Research paper thumbnail of In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320691/In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes">In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes</a></div><div class="wp-workCard_item"><span>Amino acids</span><span>, Jul 20, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-reg...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320691"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320691"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320691; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320691]").text(description); $(".js-view-count[data-work-id=60320691]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320691; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320691']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320691, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320691]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320691,"title":"In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes","translated_title":"","metadata":{"abstract":"Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...","publication_date":{"day":20,"month":7,"year":2016,"errors":{}},"publication_name":"Amino acids"},"translated_abstract":"Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...","internal_url":"https://www.academia.edu/60320691/In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes","translated_internal_url":"","created_at":"2021-10-29T08:02:14.190-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":33302,"name":"Optical Imaging","url":"https://www.academia.edu/Documents/in/Optical_Imaging"},{"id":82983,"name":"Ischemia","url":"https://www.academia.edu/Documents/in/Ischemia"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":151086,"name":"Peptides","url":"https://www.academia.edu/Documents/in/Peptides"},{"id":295928,"name":"Amino Acids","url":"https://www.academia.edu/Documents/in/Amino_Acids"},{"id":3276666,"name":"fluorescent dyes","url":"https://www.academia.edu/Documents/in/fluorescent_dyes"},{"id":3789880,"name":"Medical biochemistry and metabolomics","url":"https://www.academia.edu/Documents/in/Medical_biochemistry_and_metabolomics"},{"id":3802434,"name":"Human Umbilical Vein Endothelial Cells","url":"https://www.academia.edu/Documents/in/Human_Umbilical_Vein_Endothelial_Cells"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320690"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320690/Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_"><img alt="Research paper thumbnail of Theranostics: Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications (Small 9/2016)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320690/Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_">Theranostics: Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications (Small 9/2016)</a></div><div class="wp-workCard_item"><span>Small (Weinheim an der Bergstrasse, Germany)</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320690"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320690"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320690; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320690]").text(description); $(".js-view-count[data-work-id=60320690]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320690; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320690']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320690, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320690]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320690,"title":"Theranostics: Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications (Small 9/2016)","translated_title":"","metadata":{"abstract":"Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Small (Weinheim an der Bergstrasse, Germany)"},"translated_abstract":"Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.","internal_url":"https://www.academia.edu/60320690/Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_","translated_internal_url":"","created_at":"2021-10-29T08:02:12.231-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":733610,"name":"Small","url":"https://www.academia.edu/Documents/in/Small"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320688"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320688/Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma"><img alt="Research paper thumbnail of Theranostic gas-generating nanoparticles for targeted ultrasound imaging and treatment of neuroblastoma" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320688/Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma">Theranostic gas-generating nanoparticles for targeted ultrasound imaging and treatment of neuroblastoma</a></div><div class="wp-workCard_item"><span>Journal of controlled release : official journal of the Controlled Release Society</span><span>, Jan 29, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinic...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320688"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320688"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320688; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320688]").text(description); $(".js-view-count[data-work-id=60320688]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320688; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320688']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320688, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320688]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320688,"title":"Theranostic gas-generating nanoparticles for targeted ultrasound imaging and treatment of neuroblastoma","translated_title":"","metadata":{"abstract":"The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...","publication_date":{"day":29,"month":1,"year":2015,"errors":{}},"publication_name":"Journal of controlled release : official journal of the Controlled Release Society"},"translated_abstract":"The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...","internal_url":"https://www.academia.edu/60320688/Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma","translated_internal_url":"","created_at":"2021-10-29T08:02:10.694-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide"},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":191439,"name":"Glycoproteins","url":"https://www.academia.edu/Documents/in/Glycoproteins"},{"id":203765,"name":"Diagnostic Imaging","url":"https://www.academia.edu/Documents/in/Diagnostic_Imaging"},{"id":256805,"name":"Neuroblastoma","url":"https://www.academia.edu/Documents/in/Neuroblastoma"},{"id":630941,"name":"Calcium Carbonate","url":"https://www.academia.edu/Documents/in/Calcium_Carbonate"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":3101595,"name":"Ultrasonic Waves","url":"https://www.academia.edu/Documents/in/Ultrasonic_Waves"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320686"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320686/The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment"><img alt="Research paper thumbnail of The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320686/The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment">The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment</a></div><div class="wp-workCard_item"><span>Nanoscale</span><span>, Jan 21, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Although chitosan and its derivatives have been frequently utilized as delivery vehicles for smal...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320686"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320686"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320686; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320686]").text(description); $(".js-view-count[data-work-id=60320686]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320686; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320686']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320686, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320686]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320686,"title":"The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment","translated_title":"","metadata":{"abstract":"Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...","publication_date":{"day":21,"month":1,"year":2015,"errors":{}},"publication_name":"Nanoscale"},"translated_abstract":"Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...","internal_url":"https://www.academia.edu/60320686/The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment","translated_internal_url":"","created_at":"2021-10-29T08:02:09.088-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":9130,"name":"Chitosan","url":"https://www.academia.edu/Documents/in/Chitosan"},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation"},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":29149,"name":"Extracellular Matrix","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix"},{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques"},{"id":111112,"name":"Pneumonia","url":"https://www.academia.edu/Documents/in/Pneumonia"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":555120,"name":"Arginine","url":"https://www.academia.edu/Documents/in/Arginine"},{"id":712543,"name":"Glycine","url":"https://www.academia.edu/Documents/in/Glycine"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":2468093,"name":"Cell Membrane","url":"https://www.academia.edu/Documents/in/Cell_Membrane"},{"id":2796118,"name":"nanoscale","url":"https://www.academia.edu/Documents/in/nanoscale"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320685"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320685/Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications"><img alt="Research paper thumbnail of Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320685/Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications">Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications</a></div><div class="wp-workCard_item"><span>Small (Weinheim an der Bergstrasse, Germany)</span><span>, Jan 17, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracte...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320685"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320685"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320685; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320685]").text(description); $(".js-view-count[data-work-id=60320685]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320685; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320685']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320685, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320685]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320685,"title":"Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications","translated_title":"","metadata":{"abstract":"Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...","publication_date":{"day":17,"month":1,"year":2015,"errors":{}},"publication_name":"Small (Weinheim an der Bergstrasse, Germany)"},"translated_abstract":"Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...","internal_url":"https://www.academia.edu/60320685/Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications","translated_internal_url":"","created_at":"2021-10-29T08:02:07.461-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing"},{"id":33302,"name":"Optical Imaging","url":"https://www.academia.edu/Documents/in/Optical_Imaging"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":256805,"name":"Neuroblastoma","url":"https://www.academia.edu/Documents/in/Neuroblastoma"},{"id":733610,"name":"Small","url":"https://www.academia.edu/Documents/in/Small"},{"id":1010136,"name":"Rabies Virus","url":"https://www.academia.edu/Documents/in/Rabies_Virus"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":2950651,"name":"Tissue distribution","url":"https://www.academia.edu/Documents/in/Tissue_distribution"},{"id":3061075,"name":"Genetic Therapy","url":"https://www.academia.edu/Documents/in/Genetic_Therapy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320681"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320681/Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids"><img alt="Research paper thumbnail of Tuning the sphere-to-rod transition in the self-assembly of thermoresponsive polymer hybrids" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320681/Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids">Tuning the sphere-to-rod transition in the self-assembly of thermoresponsive polymer hybrids</a></div><div class="wp-workCard_item"><span>Colloids and surfaces. B, Biointerfaces</span><span>, Jan 9, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Nano-scale drug delivery systems have undergone extensive development, and control of size and st...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320681"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320681"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320681; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320681]").text(description); $(".js-view-count[data-work-id=60320681]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320681; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320681']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320681, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320681]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320681,"title":"Tuning the sphere-to-rod transition in the self-assembly of thermoresponsive polymer hybrids","translated_title":"","metadata":{"abstract":"Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...","publication_date":{"day":9,"month":1,"year":2015,"errors":{}},"publication_name":"Colloids and surfaces. B, Biointerfaces"},"translated_abstract":"Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...","internal_url":"https://www.academia.edu/60320681/Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids","translated_internal_url":"","created_at":"2021-10-29T08:02:05.966-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers"},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320680"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320680/Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles"><img alt="Research paper thumbnail of Molecular Imaging and Targeted Drug Delivery using Albumin-Based Nanoparticles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320680/Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles">Molecular Imaging and Targeted Drug Delivery using Albumin-Based Nanoparticles</a></div><div class="wp-workCard_item"><span>Current pharmaceutical design</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide e...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320680"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320680"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320680; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320680]").text(description); $(".js-view-count[data-work-id=60320680]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320680; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320680']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320680, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320680]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320680,"title":"Molecular Imaging and Targeted Drug Delivery using Albumin-Based Nanoparticles","translated_title":"","metadata":{"abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"Current pharmaceutical design"},"translated_abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...","internal_url":"https://www.academia.edu/60320680/Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles","translated_internal_url":"","created_at":"2021-10-29T08:02:03.450-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":39857,"name":"Molecular Imaging","url":"https://www.academia.edu/Documents/in/Molecular_Imaging"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":2349258,"name":"Serum albumin","url":"https://www.academia.edu/Documents/in/Serum_albumin"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320678"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320678/Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy"><img alt="Research paper thumbnail of Doxorubicin-Loaded Alginate-g-Poly(N-isopropylacrylamide) Micelles for Cancer Imaging and Therapy" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320678/Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy">Doxorubicin-Loaded Alginate-g-Poly(N-isopropylacrylamide) Micelles for Cancer Imaging and Therapy</a></div><div class="wp-workCard_item"><span>ACS applied materials & interfaces</span><span>, Jan 24, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often li...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320678"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320678"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320678; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320678]").text(description); $(".js-view-count[data-work-id=60320678]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320678; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320678']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320678, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320678]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320678,"title":"Doxorubicin-Loaded Alginate-g-Poly(N-isopropylacrylamide) Micelles for Cancer Imaging and Therapy","translated_title":"","metadata":{"abstract":"Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...","publication_date":{"day":24,"month":1,"year":2014,"errors":{}},"publication_name":"ACS applied materials \u0026 interfaces"},"translated_abstract":"Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...","internal_url":"https://www.academia.edu/60320678/Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy","translated_internal_url":"","created_at":"2021-10-29T08:02:00.371-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles"},{"id":83315,"name":"Diffusion","url":"https://www.academia.edu/Documents/in/Diffusion"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":300178,"name":"Nanocapsules","url":"https://www.academia.edu/Documents/in/Nanocapsules"},{"id":314240,"name":"Doxorubicin","url":"https://www.academia.edu/Documents/in/Doxorubicin"},{"id":315194,"name":"Oral Squamous Cell Carcinoma (OSCC)","url":"https://www.academia.edu/Documents/in/Oral_Squamous_Cell_Carcinoma_OSCC_"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":604754,"name":"Acrylic Resins","url":"https://www.academia.edu/Documents/in/Acrylic_Resins"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320676"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320676/Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis"><img alt="Research paper thumbnail of Co-delivery of Vascular Endothelial Growth Factor and Angiopoietin-1 Using Injectable Microsphere/Hydrogel Hybrid Systems for Therapeutic Angiogenesis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320676/Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis">Co-delivery of Vascular Endothelial Growth Factor and Angiopoietin-1 Using Injectable Microsphere/Hydrogel Hybrid Systems for Therapeutic Angiogenesis</a></div><div class="wp-workCard_item"><span>Pharmaceutical Research</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoie...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320676"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320676"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320676; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320676]").text(description); $(".js-view-count[data-work-id=60320676]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320676; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320676']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320676, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320676]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320676,"title":"Co-delivery of Vascular Endothelial Growth Factor and Angiopoietin-1 Using Injectable Microsphere/Hydrogel Hybrid Systems for Therapeutic Angiogenesis","translated_title":"","metadata":{"abstract":"We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.","publisher":"Springer Science and Business Media LLC","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Pharmaceutical Research"},"translated_abstract":"We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.","internal_url":"https://www.academia.edu/60320676/Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis","translated_internal_url":"","created_at":"2021-10-29T08:01:57.479-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical"},{"id":102727,"name":"Hydrogel","url":"https://www.academia.edu/Documents/in/Hydrogel"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1137107,"name":"Delayed-Action Preparations","url":"https://www.academia.edu/Documents/in/Delayed-Action_Preparations"},{"id":2647749,"name":"angiopoietin","url":"https://www.academia.edu/Documents/in/angiopoietin"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320673"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320673/Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System"><img alt="Research paper thumbnail of Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System" class="work-thumbnail" src="https://attachments.academia-assets.com/73819611/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320673/Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System">Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System</a></div><div class="wp-workCard_item"><span>Pharmaceutical Research</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. Methods. A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated. Results. The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system. Conclusion. A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="10a98f5ea9624572ef90f46b36d2decd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819611,"asset_id":60320673,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819611/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320673"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320673"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320673; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320673]").text(description); $(".js-view-count[data-work-id=60320673]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320673; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320673']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320673, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "10a98f5ea9624572ef90f46b36d2decd" } } $('.js-work-strip[data-work-id=60320673]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320673,"title":"Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System","translated_title":"","metadata":{"publisher":"Springer Nature","grobid_abstract":"Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. Methods. A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated. Results. The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system. Conclusion. A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Pharmaceutical Research","grobid_abstract_attachment_id":73819611},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320673/Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System","translated_internal_url":"","created_at":"2021-10-29T08:01:54.657-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819611,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819611/thumbnails/1.jpg","file_name":"s11095-010-0067-020211029-14358-ov94fs.pdf","download_url":"https://www.academia.edu/attachments/73819611/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Active_Blood_Vessel_Formation_in_the_Isc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819611/s11095-010-0067-020211029-14358-ov94fs-libre.pdf?1635521172=\u0026response-content-disposition=attachment%3B+filename%3DActive_Blood_Vessel_Formation_in_the_Isc.pdf\u0026Expires=1734563129\u0026Signature=H-aVxS~M8hb5gKS4gXXph7n0X7BihVIJ7pXrldZlV8QrWq9eCPVoNjbUoGDQJ8fnEdYf~P0io~kpozHcbVMn9T6RldA~UeLk86ilDqMfNEnf8VWswqELrzhGG8QLUhBdRbGYWx6H4MfT7crdw5MhX6EdPbG5CA2LmmJ2aTBzvP4hacigjl436XODDLelu2w0r4ZL8xcE9aa59t8Y2SEyO2WuPbQy-F4-UFjR4RdGw2CggvE117w4jRvlqEAVAVnMDxDk1jWw6je4sG1nMM3rlMpCh3Nbovx0ouUdyb4c2ydvLswYwnjZd4hpMCFaEFtDXST1hrR8Zhe7lpVFK~jApg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. Methods. A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated. Results. The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system. Conclusion. A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819611,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819611/thumbnails/1.jpg","file_name":"s11095-010-0067-020211029-14358-ov94fs.pdf","download_url":"https://www.academia.edu/attachments/73819611/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Active_Blood_Vessel_Formation_in_the_Isc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819611/s11095-010-0067-020211029-14358-ov94fs-libre.pdf?1635521172=\u0026response-content-disposition=attachment%3B+filename%3DActive_Blood_Vessel_Formation_in_the_Isc.pdf\u0026Expires=1734563129\u0026Signature=H-aVxS~M8hb5gKS4gXXph7n0X7BihVIJ7pXrldZlV8QrWq9eCPVoNjbUoGDQJ8fnEdYf~P0io~kpozHcbVMn9T6RldA~UeLk86ilDqMfNEnf8VWswqELrzhGG8QLUhBdRbGYWx6H4MfT7crdw5MhX6EdPbG5CA2LmmJ2aTBzvP4hacigjl436XODDLelu2w0r4ZL8xcE9aa59t8Y2SEyO2WuPbQy-F4-UFjR4RdGw2CggvE117w4jRvlqEAVAVnMDxDk1jWw6je4sG1nMM3rlMpCh3Nbovx0ouUdyb4c2ydvLswYwnjZd4hpMCFaEFtDXST1hrR8Zhe7lpVFK~jApg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry"},{"id":22442,"name":"Hydrogels","url":"https://www.academia.edu/Documents/in/Hydrogels"},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":82983,"name":"Ischemia","url":"https://www.academia.edu/Documents/in/Ischemia"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1135766,"name":"Excipients","url":"https://www.academia.edu/Documents/in/Excipients"},{"id":1137107,"name":"Delayed-Action Preparations","url":"https://www.academia.edu/Documents/in/Delayed-Action_Preparations"},{"id":1796877,"name":"Vascular Endothelial Growth Factor","url":"https://www.academia.edu/Documents/in/Vascular_Endothelial_Growth_Factor"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320672"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320672/Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System"><img alt="Research paper thumbnail of Local and Sustained Vascular Endothelial Growth Factor Delivery for Angiogenesis Using an Injectable System" class="work-thumbnail" src="https://attachments.academia-assets.com/73819692/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320672/Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System">Local and Sustained Vascular Endothelial Growth Factor Delivery for Angiogenesis Using an Injectable System</a></div><div class="wp-workCard_item"><span>Pharmaceutical Research</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the lo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the local and sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis in vivo. Methods. Poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing rhVEGF were loaded into alginate gels by ionic cross-linking. The rhVEGF release from the system was monitored and bioactivity was tested in vitro. The combination system was subcutaneously injected into mice using a syringe, and new blood vessel formation was evaluated. Results. Sustained rhVEGF release from the combination system was observed for 3 weeks, and the released rhVEGF remained bioactive. Endothelial cell proliferation was significantly enhanced when cells were cultured with the rhVEGF-releasing combination system in vitro. When the combination system was implanted, the granulation tissue layer was thicker with more newly formed blood vessels than that with a single dose VEGF injection. Conclusion. The rhVEGF release was controlled by varying relative portions of microspheres and hydrogels in combination delivery systems, which efficiently promoted new blood vessel formation in vivo. This combination system could be a promising delivery vehicle for therapeutic angiogenesis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="770cf83025e1b28c1772e1a829364400" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819692,"asset_id":60320672,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819692/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320672"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320672"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320672; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320672]").text(description); $(".js-view-count[data-work-id=60320672]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320672; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320672']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320672, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "770cf83025e1b28c1772e1a829364400" } } $('.js-work-strip[data-work-id=60320672]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320672,"title":"Local and Sustained Vascular Endothelial Growth Factor Delivery for Angiogenesis Using an Injectable System","translated_title":"","metadata":{"publisher":"Springer Nature","grobid_abstract":"Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the local and sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis in vivo. Methods. Poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing rhVEGF were loaded into alginate gels by ionic cross-linking. The rhVEGF release from the system was monitored and bioactivity was tested in vitro. The combination system was subcutaneously injected into mice using a syringe, and new blood vessel formation was evaluated. Results. Sustained rhVEGF release from the combination system was observed for 3 weeks, and the released rhVEGF remained bioactive. Endothelial cell proliferation was significantly enhanced when cells were cultured with the rhVEGF-releasing combination system in vitro. When the combination system was implanted, the granulation tissue layer was thicker with more newly formed blood vessels than that with a single dose VEGF injection. Conclusion. The rhVEGF release was controlled by varying relative portions of microspheres and hydrogels in combination delivery systems, which efficiently promoted new blood vessel formation in vivo. This combination system could be a promising delivery vehicle for therapeutic angiogenesis.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}},"publication_name":"Pharmaceutical Research","grobid_abstract_attachment_id":73819692},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320672/Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System","translated_internal_url":"","created_at":"2021-10-29T08:01:52.591-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819692,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819692/thumbnails/1.jpg","file_name":"s11095-009-9884-420211029-14352-gt5w09.pdf","download_url":"https://www.academia.edu/attachments/73819692/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Local_and_Sustained_Vascular_Endothelial.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819692/s11095-009-9884-420211029-14352-gt5w09-libre.pdf?1635521168=\u0026response-content-disposition=attachment%3B+filename%3DLocal_and_Sustained_Vascular_Endothelial.pdf\u0026Expires=1734563129\u0026Signature=WK-YiBV3C1M5m3AHbXJ7Sw6QS~7NTF2d2NEY~8pwv3bgy0a2KSz5vHKkH~xBHj0U8Yu8Z6PrLYShvoM7SKMzugQ70bcxePzYLigPhX8JlbYEvFNLXAnPntD1qgUK2E9GGBEeIEcvhR8XrTnK5oIHEVNyrChRGb9WZbfAHoz5QAGpe6xBM21GWAJPO5U0BvOYMYCyvrKNF1LY569JwjgK2GVaCARmnK2Gh0rRdLAr8C21n19v9f4-5JThaE1--X9DXyP3ptX5pTUtkg6Sd4tnwHhxHxioyuhZkgNHx~OOi8LvoiCLjSpBtkeC1preeGUGUjhFASIUOiUdekpPlmjYqw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the local and sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis in vivo. Methods. Poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing rhVEGF were loaded into alginate gels by ionic cross-linking. The rhVEGF release from the system was monitored and bioactivity was tested in vitro. The combination system was subcutaneously injected into mice using a syringe, and new blood vessel formation was evaluated. Results. Sustained rhVEGF release from the combination system was observed for 3 weeks, and the released rhVEGF remained bioactive. Endothelial cell proliferation was significantly enhanced when cells were cultured with the rhVEGF-releasing combination system in vitro. When the combination system was implanted, the granulation tissue layer was thicker with more newly formed blood vessels than that with a single dose VEGF injection. Conclusion. The rhVEGF release was controlled by varying relative portions of microspheres and hydrogels in combination delivery systems, which efficiently promoted new blood vessel formation in vivo. This combination system could be a promising delivery vehicle for therapeutic angiogenesis.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819692,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819692/thumbnails/1.jpg","file_name":"s11095-009-9884-420211029-14352-gt5w09.pdf","download_url":"https://www.academia.edu/attachments/73819692/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Local_and_Sustained_Vascular_Endothelial.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819692/s11095-009-9884-420211029-14352-gt5w09-libre.pdf?1635521168=\u0026response-content-disposition=attachment%3B+filename%3DLocal_and_Sustained_Vascular_Endothelial.pdf\u0026Expires=1734563129\u0026Signature=WK-YiBV3C1M5m3AHbXJ7Sw6QS~7NTF2d2NEY~8pwv3bgy0a2KSz5vHKkH~xBHj0U8Yu8Z6PrLYShvoM7SKMzugQ70bcxePzYLigPhX8JlbYEvFNLXAnPntD1qgUK2E9GGBEeIEcvhR8XrTnK5oIHEVNyrChRGb9WZbfAHoz5QAGpe6xBM21GWAJPO5U0BvOYMYCyvrKNF1LY569JwjgK2GVaCARmnK2Gh0rRdLAr8C21n19v9f4-5JThaE1--X9DXyP3ptX5pTUtkg6Sd4tnwHhxHxioyuhZkgNHx~OOi8LvoiCLjSpBtkeC1preeGUGUjhFASIUOiUdekpPlmjYqw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22442,"name":"Hydrogels","url":"https://www.academia.edu/Documents/in/Hydrogels"},{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1796877,"name":"Vascular Endothelial Growth Factor","url":"https://www.academia.edu/Documents/in/Vascular_Endothelial_Growth_Factor"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320671"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320671/Injectable_Microsphere_Hydrogel_Combination_Systems_for_Localized_Protein_Delivery"><img alt="Research paper thumbnail of Injectable Microsphere/Hydrogel Combination Systems for Localized Protein Delivery" class="work-thumbnail" src="https://attachments.academia-assets.com/73819617/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320671/Injectable_Microsphere_Hydrogel_Combination_Systems_for_Localized_Protein_Delivery">Injectable Microsphere/Hydrogel Combination Systems for Localized Protein Delivery</a></div><div class="wp-workCard_item"><span>Macromolecular Bioscience</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bb9bc0704da0a8157ee88a812ca30818" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819617,"asset_id":60320671,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819617/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320671"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320671"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320671; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320671]").text(description); $(".js-view-count[data-work-id=60320671]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320671; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320671']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320671, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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By preparing PLGA microspheres containing the model protein and combining them with alginate gels, the study hypothesizes that the mixing ratios of these components can control protein release. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320669"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320669/Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model"><img alt="Research paper thumbnail of Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320669/Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model">Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model</a></div><div class="wp-workCard_item"><span>Journal of Drug Targeting</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury an...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320669"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320669"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320669; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320669]").text(description); $(".js-view-count[data-work-id=60320669]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320669; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320669']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320669, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320669]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320669,"title":"Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model","translated_title":"","metadata":{"abstract":"Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.","publisher":"Informa UK Limited","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Journal of Drug Targeting"},"translated_abstract":"Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.","internal_url":"https://www.academia.edu/60320669/Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model","translated_internal_url":"","created_at":"2021-10-29T08:01:49.351-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":22442,"name":"Hydrogels","url":"https://www.academia.edu/Documents/in/Hydrogels"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats"},{"id":378016,"name":"Myocardial Infarction","url":"https://www.academia.edu/Documents/in/Myocardial_Infarction"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":765872,"name":"Heat Shock Protein","url":"https://www.academia.edu/Documents/in/Heat_Shock_Protein"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1015099,"name":"Drug Targeting","url":"https://www.academia.edu/Documents/in/Drug_Targeting"},{"id":1031068,"name":"Drug Carriers","url":"https://www.academia.edu/Documents/in/Drug_Carriers"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1137107,"name":"Delayed-Action Preparations","url":"https://www.academia.edu/Documents/in/Delayed-Action_Preparations"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320668"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320668/Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction"><img alt="Research paper thumbnail of Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction" class="work-thumbnail" src="https://attachments.academia-assets.com/73819679/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320668/Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction">Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock pro...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. In this study, in order to enhance the penetration of heat shock protein 27 (HSP27) across cell membranes, we fused HSP27 with transcriptional activator (TAT) derived from human immunodeficiency virus (HIV) as a protein transduction domain (PTD). We loaded the fusion protein (TAT-HSP27) into microsphere/hydrogel combination delivery systems to control the release behavior for prolonged time periods. We found that the release behavior of TAT-HSP27 was able to be controlled by varying the ratio of PLGA microspheres and alginate hydrogels. Indeed, the released fusion protein maintained its bioactivity and could recover the proliferation of cardiomyoblasts cultured under hypoxic conditions. This approach to controlling the release behavior of TAT-HSP27 using microsphere/hydrogel combination delivery systems may be useful for treating myocardial infarction in a minimally invasive manner.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5e0e3c83a63d5b877b36592715040756" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819679,"asset_id":60320668,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819679/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320668"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320668"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320668; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320668]").text(description); $(".js-view-count[data-work-id=60320668]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320668; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320668']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320668, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5e0e3c83a63d5b877b36592715040756" } } $('.js-work-strip[data-work-id=60320668]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320668,"title":"Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. In this study, in order to enhance the penetration of heat shock protein 27 (HSP27) across cell membranes, we fused HSP27 with transcriptional activator (TAT) derived from human immunodeficiency virus (HIV) as a protein transduction domain (PTD). We loaded the fusion protein (TAT-HSP27) into microsphere/hydrogel combination delivery systems to control the release behavior for prolonged time periods. We found that the release behavior of TAT-HSP27 was able to be controlled by varying the ratio of PLGA microspheres and alginate hydrogels. Indeed, the released fusion protein maintained its bioactivity and could recover the proliferation of cardiomyoblasts cultured under hypoxic conditions. This approach to controlling the release behavior of TAT-HSP27 using microsphere/hydrogel combination delivery systems may be useful for treating myocardial infarction in a minimally invasive manner.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}},"publication_name":"Journal of Controlled Release","grobid_abstract_attachment_id":73819679},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320668/Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction","translated_internal_url":"","created_at":"2021-10-29T08:01:46.845-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819679,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819679/thumbnails/1.jpg","file_name":"j.jconrel.2009.04.00820211029-14352-1pwc6he.pdf","download_url":"https://www.academia.edu/attachments/73819679/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Controlled_delivery_of_heat_shock_protei.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819679/j.jconrel.2009.04.00820211029-14352-1pwc6he-libre.pdf?1635521169=\u0026response-content-disposition=attachment%3B+filename%3DControlled_delivery_of_heat_shock_protei.pdf\u0026Expires=1734563129\u0026Signature=OIPL3g9bikrQP726tKEDmHlSwjeGevMCwaVo2Sv8CSRWroME5KEvuY4WTcfinxc4ywRd015uru6~Sjp4Z~Yw8~opow4IlX3Yn5incdO1J-~SALDe4dvKtyTZ3e2VuOYWvUpcr4S0xYdc6HZedt~EXh0J9Q4rTQpRBFSm7QlLZladORHroUAGsIV8vDhvq~pLVjzt529NEeA49lhNc1JRUtaFdBrovfCaF6U7bZbotgZTrlj6W54x1O6uyV8S2Yg8K9Ez0Jl8P7ruXSceuElWFw13BHSw0kcjXRMtuPZcEsFmdN70HKaVWZFk5u6-A-8k9-NYEh0KCpmhWQbQ51d9GQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":"Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. In this study, in order to enhance the penetration of heat shock protein 27 (HSP27) across cell membranes, we fused HSP27 with transcriptional activator (TAT) derived from human immunodeficiency virus (HIV) as a protein transduction domain (PTD). We loaded the fusion protein (TAT-HSP27) into microsphere/hydrogel combination delivery systems to control the release behavior for prolonged time periods. We found that the release behavior of TAT-HSP27 was able to be controlled by varying the ratio of PLGA microspheres and alginate hydrogels. Indeed, the released fusion protein maintained its bioactivity and could recover the proliferation of cardiomyoblasts cultured under hypoxic conditions. This approach to controlling the release behavior of TAT-HSP27 using microsphere/hydrogel combination delivery systems may be useful for treating myocardial infarction in a minimally invasive manner.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819679,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819679/thumbnails/1.jpg","file_name":"j.jconrel.2009.04.00820211029-14352-1pwc6he.pdf","download_url":"https://www.academia.edu/attachments/73819679/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Controlled_delivery_of_heat_shock_protei.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819679/j.jconrel.2009.04.00820211029-14352-1pwc6he-libre.pdf?1635521169=\u0026response-content-disposition=attachment%3B+filename%3DControlled_delivery_of_heat_shock_protei.pdf\u0026Expires=1734563129\u0026Signature=OIPL3g9bikrQP726tKEDmHlSwjeGevMCwaVo2Sv8CSRWroME5KEvuY4WTcfinxc4ywRd015uru6~Sjp4Z~Yw8~opow4IlX3Yn5incdO1J-~SALDe4dvKtyTZ3e2VuOYWvUpcr4S0xYdc6HZedt~EXh0J9Q4rTQpRBFSm7QlLZladORHroUAGsIV8vDhvq~pLVjzt529NEeA49lhNc1JRUtaFdBrovfCaF6U7bZbotgZTrlj6W54x1O6uyV8S2Yg8K9Ez0Jl8P7ruXSceuElWFw13BHSw0kcjXRMtuPZcEsFmdN70HKaVWZFk5u6-A-8k9-NYEh0KCpmhWQbQ51d9GQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV"},{"id":41747,"name":"Dosage Form","url":"https://www.academia.edu/Documents/in/Dosage_Form"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":102727,"name":"Hydrogel","url":"https://www.academia.edu/Documents/in/Hydrogel"},{"id":318308,"name":"Human immunodeficiency virus","url":"https://www.academia.edu/Documents/in/Human_immunodeficiency_virus"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":421276,"name":"Delivery System","url":"https://www.academia.edu/Documents/in/Delivery_System"},{"id":556132,"name":"Microsphere","url":"https://www.academia.edu/Documents/in/Microsphere"},{"id":575076,"name":"Heat Shock","url":"https://www.academia.edu/Documents/in/Heat_Shock"},{"id":765872,"name":"Heat Shock Protein","url":"https://www.academia.edu/Documents/in/Heat_Shock_Protein"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":1159037,"name":"Fusion Protein","url":"https://www.academia.edu/Documents/in/Fusion_Protein"},{"id":2468093,"name":"Cell Membrane","url":"https://www.academia.edu/Documents/in/Cell_Membrane"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320666"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320666/Facile_control_of_porous_structures_of_polymer_microspheres_using_an_osmotic_agent_for_pulmonary_delivery"><img alt="Research paper thumbnail of Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery" class="work-thumbnail" src="https://attachments.academia-assets.com/73819675/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320666/Facile_control_of_porous_structures_of_polymer_microspheres_using_an_osmotic_agent_for_pulmonary_delivery">Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">It has been challenging to prepare polymeric microspheres with controlled porous structures for m...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">It has been challenging to prepare polymeric microspheres with controlled porous structures for many biomedical applications, particularly for pulmonary drug delivery. Here, we report the use of bovine serum albumin (BSA) as an osmotic agent in order to control the porous structure of poly(D,L-lactide-co-glycolide) (PLGA) microspheres prepared by a double emulsion method. BSA was useful to induce osmosis between internal and external water phases during the double emulsion process, resulting in the fabrication of microspheres with controllable, uniform porous structures. The pore size of PLGA microspheres was controlled independently from the particle size by this approach. The use of BSA as an osmotic agent reduced the initial burst of model proteins (e.g., insulin and VEGF) entrapped in the porous microspheres, and the sustained release of VEGF was achieved for two weeks in vitro. This approach to controlling porous structures of polymer microspheres could be useful to develop novel pulmonary drug delivery systems.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="29b9d99bb0b6d35fe48e358d1c17fac4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819675,"asset_id":60320666,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819675/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320666"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320666"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320666; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320666]").text(description); $(".js-view-count[data-work-id=60320666]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320666; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320666']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320666, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "29b9d99bb0b6d35fe48e358d1c17fac4" } } $('.js-work-strip[data-work-id=60320666]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320666,"title":"Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"It has been challenging to prepare polymeric microspheres with controlled porous structures for many biomedical applications, particularly for pulmonary drug delivery. Here, we report the use of bovine serum albumin (BSA) as an osmotic agent in order to control the porous structure of poly(D,L-lactide-co-glycolide) (PLGA) microspheres prepared by a double emulsion method. BSA was useful to induce osmosis between internal and external water phases during the double emulsion process, resulting in the fabrication of microspheres with controllable, uniform porous structures. The pore size of PLGA microspheres was controlled independently from the particle size by this approach. The use of BSA as an osmotic agent reduced the initial burst of model proteins (e.g., insulin and VEGF) entrapped in the porous microspheres, and the sustained release of VEGF was achieved for two weeks in vitro. This approach to controlling porous structures of polymer microspheres could be useful to develop novel pulmonary drug delivery systems.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Journal of Controlled Release","grobid_abstract_attachment_id":73819675},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320666/Facile_control_of_porous_structures_of_polymer_microspheres_using_an_osmotic_agent_for_pulmonary_delivery","translated_internal_url":"","created_at":"2021-10-29T08:01:45.128-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819675,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819675/thumbnails/1.jpg","file_name":"j.jconrel.2010.05.02620211029-15830-359juu.pdf","download_url":"https://www.academia.edu/attachments/73819675/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Facile_control_of_porous_structures_of_p.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819675/j.jconrel.2010.05.02620211029-15830-359juu-libre.pdf?1635521170=\u0026response-content-disposition=attachment%3B+filename%3DFacile_control_of_porous_structures_of_p.pdf\u0026Expires=1734563129\u0026Signature=SLfF8aD4O18c8dsT9cGBENJzuBQbul25HSUTBotpuW1ZR9yD0CYk-eUEG39vMykq3Hf3nwJvecCNE6oCuNguFFfKtNxX4RYJPXaZw3aIEjFyXeH~UGJus1f6tQeuGivk6Yv~mo0ifQe~FhH-CzmFpgjWOeB~cDyyhfZ82Hwn0-GL1hBqolvM6xMLfLe0A-Dj7-HYA4X8LasQrE7y1PUNOqPkrgGZmO5qhcpQz89DhizHhL7t99cg5KEmuYRdEVbC9R86OispJpH9e2UJAmKhajfpe9bFReha6XFu0aLcchXKft31tqbwJrRQxVj87GhOsK7h4K~iOJJmTnAmujTRLw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Facile_control_of_porous_structures_of_polymer_microspheres_using_an_osmotic_agent_for_pulmonary_delivery","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":"It has been challenging to prepare polymeric microspheres with controlled porous structures for many biomedical applications, particularly for pulmonary drug delivery. Here, we report the use of bovine serum albumin (BSA) as an osmotic agent in order to control the porous structure of poly(D,L-lactide-co-glycolide) (PLGA) microspheres prepared by a double emulsion method. BSA was useful to induce osmosis between internal and external water phases during the double emulsion process, resulting in the fabrication of microspheres with controllable, uniform porous structures. The pore size of PLGA microspheres was controlled independently from the particle size by this approach. The use of BSA as an osmotic agent reduced the initial burst of model proteins (e.g., insulin and VEGF) entrapped in the porous microspheres, and the sustained release of VEGF was achieved for two weeks in vitro. This approach to controlling porous structures of polymer microspheres could be useful to develop novel pulmonary drug delivery systems.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819675,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819675/thumbnails/1.jpg","file_name":"j.jconrel.2010.05.02620211029-15830-359juu.pdf","download_url":"https://www.academia.edu/attachments/73819675/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Facile_control_of_porous_structures_of_p.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819675/j.jconrel.2010.05.02620211029-15830-359juu-libre.pdf?1635521170=\u0026response-content-disposition=attachment%3B+filename%3DFacile_control_of_porous_structures_of_p.pdf\u0026Expires=1734563129\u0026Signature=SLfF8aD4O18c8dsT9cGBENJzuBQbul25HSUTBotpuW1ZR9yD0CYk-eUEG39vMykq3Hf3nwJvecCNE6oCuNguFFfKtNxX4RYJPXaZw3aIEjFyXeH~UGJus1f6tQeuGivk6Yv~mo0ifQe~FhH-CzmFpgjWOeB~cDyyhfZ82Hwn0-GL1hBqolvM6xMLfLe0A-Dj7-HYA4X8LasQrE7y1PUNOqPkrgGZmO5qhcpQz89DhizHhL7t99cg5KEmuYRdEVbC9R86OispJpH9e2UJAmKhajfpe9bFReha6XFu0aLcchXKft31tqbwJrRQxVj87GhOsK7h4K~iOJJmTnAmujTRLw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":68315,"name":"Porosity","url":"https://www.academia.edu/Documents/in/Porosity"},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":477103,"name":"Emulsions","url":"https://www.academia.edu/Documents/in/Emulsions"},{"id":556132,"name":"Microsphere","url":"https://www.academia.edu/Documents/in/Microsphere"},{"id":609176,"name":"Osmosis","url":"https://www.academia.edu/Documents/in/Osmosis"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":881608,"name":"Bovine Serum Albumin","url":"https://www.academia.edu/Documents/in/Bovine_Serum_Albumin"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1031068,"name":"Drug Carriers","url":"https://www.academia.edu/Documents/in/Drug_Carriers"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1135812,"name":"Drug Compounding","url":"https://www.academia.edu/Documents/in/Drug_Compounding"},{"id":1136192,"name":"Biological Availability","url":"https://www.academia.edu/Documents/in/Biological_Availability"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320663"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320663/Preparation_of_budesonide_loaded_porous_PLGA_microparticles_and_their_therapeutic_efficacy_in_a_murine_asthma_model"><img alt="Research paper thumbnail of Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320663/Preparation_of_budesonide_loaded_porous_PLGA_microparticles_and_their_therapeutic_efficacy_in_a_murine_asthma_model">Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. Howev...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320663"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320663"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320663; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320663]").text(description); $(".js-view-count[data-work-id=60320663]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320663; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320663']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320663, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320663]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320663,"title":"Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model","translated_title":"","metadata":{"abstract":"Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2011,"errors":{}},"publication_name":"Journal of Controlled Release"},"translated_abstract":"Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.","internal_url":"https://www.academia.edu/60320663/Preparation_of_budesonide_loaded_porous_PLGA_microparticles_and_their_therapeutic_efficacy_in_a_murine_asthma_model","translated_internal_url":"","created_at":"2021-10-29T08:01:44.351-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Preparation_of_budesonide_loaded_porous_PLGA_microparticles_and_their_therapeutic_efficacy_in_a_murine_asthma_model","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"},{"id":8942,"name":"Treatment","url":"https://www.academia.edu/Documents/in/Treatment"},{"id":9968,"name":"Asthma","url":"https://www.academia.edu/Documents/in/Asthma"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":68315,"name":"Porosity","url":"https://www.academia.edu/Documents/in/Porosity"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung"},{"id":219927,"name":"Efficiency","url":"https://www.academia.edu/Documents/in/Efficiency"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":698785,"name":"Side Effect","url":"https://www.academia.edu/Documents/in/Side_Effect"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1031068,"name":"Drug Carriers","url":"https://www.academia.edu/Documents/in/Drug_Carriers"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1232391,"name":"Budesonide","url":"https://www.academia.edu/Documents/in/Budesonide"},{"id":1291661,"name":"Copolymer","url":"https://www.academia.edu/Documents/in/Copolymer"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"},{"id":4040940,"name":"Bicarbonates","url":"https://www.academia.edu/Documents/in/Bicarbonates"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320661"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320661/Single_chain_variable_fragment_CD7_antibody_conjugated_PLGA_HDAC_inhibitor_immuno_nanoparticles_Developing_human_T_cell_specific_nano_technology_for_delivery_of_therapeutic_drugs_targeting_latent_HIV"><img alt="Research paper thumbnail of Single chain variable fragment CD7 antibody conjugated PLGA/HDAC inhibitor immuno-nanoparticles: Developing human T cell-specific nano-technology for delivery of therapeutic drugs targeting latent HIV" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320661/Single_chain_variable_fragment_CD7_antibody_conjugated_PLGA_HDAC_inhibitor_immuno_nanoparticles_Developing_human_T_cell_specific_nano_technology_for_delivery_of_therapeutic_drugs_targeting_latent_HIV">Single chain variable fragment CD7 antibody conjugated PLGA/HDAC inhibitor immuno-nanoparticles: Developing human T cell-specific nano-technology for delivery of therapeutic drugs targeting latent HIV</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320661"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320661"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320661; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="12759458" id="papers"><div class="js-work-strip profile--work_container" data-work-id="110578274"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/110578274/Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size"><img alt="Research paper thumbnail of Two distinct cellular pathways leading to endothelial cell cytotoxicity by silica nanoparticle size" class="work-thumbnail" src="https://attachments.academia-assets.com/108352132/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/110578274/Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size">Two distinct cellular pathways leading to endothelial cell cytotoxicity by silica nanoparticle size</a></div><div class="wp-workCard_item"><span>Journal of Nanobiotechnology</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for the targeted delivery of therapeutic agents. Safety concerns about the biomedical and clinical applications of SiNPs have been raised, necessitating analysis of the effects of their intrinsic properties, such as sizes, shapes, and surface physicochemical characteristics, on human health to minimize risk in biomedical applications. In particular, SiNP size-associated toxicological effects, and the underlying molecular mechanisms in the vascular endothelium remain unclear. This study aimed to elucidate the detailed mechanisms underlying the cellular response to exposure to trace amounts of SiNPs and to determine applicable size criteria for biomedical application. Methods: To clarify whether these SiNP-mediated cytotoxicity due to induction of apoptosis or necrosis, human ECs were treated with SiNPs of four different non-overlapping sizes under low serum-containing condition, stained with annexin V and propidium iodide (PI), and subjected to flow cytometric analysis (FACS). Two types of cell death mechanisms were assessed in terms of production of reactive oxygen species (ROS), endoplasmic reticulum (ER) stress induction, and autophagy activity. Results: Spherical SiNPs had a diameter of 21.8 nm; this was further increased to 31.4, 42.9, and 56.7 nm. Hence, we investigated these effects in human endothelial cells (ECs) treated with these nanoparticles under overlap-or agglomerate-free conditions. The 20-nm SiNPs, but not SiNPs of other sizes, significantly induced apoptosis and necrosis. Surprisingly, the two types of cell death occurred independently and through different mechanisms. Apoptotic cell death resulted from ROS-mediated ER stress. Furthermore, autophagy-mediated necrotic cell death was induced through the PI3K/AKT/eNOS signaling axis. Together, the present results indicate that SiNPs within a diameter of < 20-nm pose greater risks to cells in terms of cytotoxic effects.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="af1840bb7751995e503f826209485569" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":108352132,"asset_id":110578274,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/108352132/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="110578274"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="110578274"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 110578274; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=110578274]").text(description); $(".js-view-count[data-work-id=110578274]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 110578274; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='110578274']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 110578274, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "af1840bb7751995e503f826209485569" } } $('.js-work-strip[data-work-id=110578274]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":110578274,"title":"Two distinct cellular pathways leading to endothelial cell cytotoxicity by silica nanoparticle size","translated_title":"","metadata":{"publisher":"Springer Science and Business Media LLC","grobid_abstract":"Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for the targeted delivery of therapeutic agents. Safety concerns about the biomedical and clinical applications of SiNPs have been raised, necessitating analysis of the effects of their intrinsic properties, such as sizes, shapes, and surface physicochemical characteristics, on human health to minimize risk in biomedical applications. In particular, SiNP size-associated toxicological effects, and the underlying molecular mechanisms in the vascular endothelium remain unclear. This study aimed to elucidate the detailed mechanisms underlying the cellular response to exposure to trace amounts of SiNPs and to determine applicable size criteria for biomedical application. Methods: To clarify whether these SiNP-mediated cytotoxicity due to induction of apoptosis or necrosis, human ECs were treated with SiNPs of four different non-overlapping sizes under low serum-containing condition, stained with annexin V and propidium iodide (PI), and subjected to flow cytometric analysis (FACS). Two types of cell death mechanisms were assessed in terms of production of reactive oxygen species (ROS), endoplasmic reticulum (ER) stress induction, and autophagy activity. Results: Spherical SiNPs had a diameter of 21.8 nm; this was further increased to 31.4, 42.9, and 56.7 nm. Hence, we investigated these effects in human endothelial cells (ECs) treated with these nanoparticles under overlap-or agglomerate-free conditions. The 20-nm SiNPs, but not SiNPs of other sizes, significantly induced apoptosis and necrosis. Surprisingly, the two types of cell death occurred independently and through different mechanisms. Apoptotic cell death resulted from ROS-mediated ER stress. Furthermore, autophagy-mediated necrotic cell death was induced through the PI3K/AKT/eNOS signaling axis. Together, the present results indicate that SiNPs within a diameter of \u003c 20-nm pose greater risks to cells in terms of cytotoxic effects.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"Journal of Nanobiotechnology","grobid_abstract_attachment_id":108352132},"translated_abstract":null,"internal_url":"https://www.academia.edu/110578274/Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size","translated_internal_url":"","created_at":"2023-12-04T15:10:54.059-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":108352132,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/108352132/thumbnails/1.jpg","file_name":"s12951-019-0456-4.pdf","download_url":"https://www.academia.edu/attachments/108352132/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Two_distinct_cellular_pathways_leading_t.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/108352132/s12951-019-0456-4-libre.pdf?1701732329=\u0026response-content-disposition=attachment%3B+filename%3DTwo_distinct_cellular_pathways_leading_t.pdf\u0026Expires=1734563128\u0026Signature=QcKKxegpgeMpmAVqMeZETlWx4HgATuTd-MrNgnApSzkG-shYAt~S4WHu5Wiohbcl1YgL~mR9iHf0SwoIbni0naga~QQtbcreUNmmO2JuvOejEKFIyMQNgkFq0Qd5gNfdUrwKKTJ3savss0o5Q6eKJ1Us4Wy68WUP9AUtgHHA74cDj51QsldcQWxvX4knwFvvUw0u9RW7W0yRB4G9OKLRHtSBYsAkY9wWHONtBfZaUQfe0CWWy6VHj7yxi45vY2IuGmW6~eUH8prndM4kehDgiC-NQFP0fsI-T4gPTcxLDTz0Ka5BdhGFD2WnCS4~l9pY2W42E9qXc3MY~hTb--ovMw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Two_distinct_cellular_pathways_leading_to_endothelial_cell_cytotoxicity_by_silica_nanoparticle_size","translated_slug":"","page_count":14,"language":"en","content_type":"Work","summary":"Background: Silica nanoparticles (SiNPs) are widely used for biosensing and diagnostics, and for the targeted delivery of therapeutic agents. Safety concerns about the biomedical and clinical applications of SiNPs have been raised, necessitating analysis of the effects of their intrinsic properties, such as sizes, shapes, and surface physicochemical characteristics, on human health to minimize risk in biomedical applications. In particular, SiNP size-associated toxicological effects, and the underlying molecular mechanisms in the vascular endothelium remain unclear. This study aimed to elucidate the detailed mechanisms underlying the cellular response to exposure to trace amounts of SiNPs and to determine applicable size criteria for biomedical application. Methods: To clarify whether these SiNP-mediated cytotoxicity due to induction of apoptosis or necrosis, human ECs were treated with SiNPs of four different non-overlapping sizes under low serum-containing condition, stained with annexin V and propidium iodide (PI), and subjected to flow cytometric analysis (FACS). Two types of cell death mechanisms were assessed in terms of production of reactive oxygen species (ROS), endoplasmic reticulum (ER) stress induction, and autophagy activity. Results: Spherical SiNPs had a diameter of 21.8 nm; this was further increased to 31.4, 42.9, and 56.7 nm. Hence, we investigated these effects in human endothelial cells (ECs) treated with these nanoparticles under overlap-or agglomerate-free conditions. The 20-nm SiNPs, but not SiNPs of other sizes, significantly induced apoptosis and necrosis. Surprisingly, the two types of cell death occurred independently and through different mechanisms. Apoptotic cell death resulted from ROS-mediated ER stress. Furthermore, autophagy-mediated necrotic cell death was induced through the PI3K/AKT/eNOS signaling axis. Together, the present results indicate that SiNPs within a diameter of \u003c 20-nm pose greater risks to cells in terms of cytotoxic effects.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":108352132,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/108352132/thumbnails/1.jpg","file_name":"s12951-019-0456-4.pdf","download_url":"https://www.academia.edu/attachments/108352132/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Two_distinct_cellular_pathways_leading_t.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/108352132/s12951-019-0456-4-libre.pdf?1701732329=\u0026response-content-disposition=attachment%3B+filename%3DTwo_distinct_cellular_pathways_leading_t.pdf\u0026Expires=1734563128\u0026Signature=QcKKxegpgeMpmAVqMeZETlWx4HgATuTd-MrNgnApSzkG-shYAt~S4WHu5Wiohbcl1YgL~mR9iHf0SwoIbni0naga~QQtbcreUNmmO2JuvOejEKFIyMQNgkFq0Qd5gNfdUrwKKTJ3savss0o5Q6eKJ1Us4Wy68WUP9AUtgHHA74cDj51QsldcQWxvX4knwFvvUw0u9RW7W0yRB4G9OKLRHtSBYsAkY9wWHONtBfZaUQfe0CWWy6VHj7yxi45vY2IuGmW6~eUH8prndM4kehDgiC-NQFP0fsI-T4gPTcxLDTz0Ka5BdhGFD2WnCS4~l9pY2W42E9qXc3MY~hTb--ovMw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology"},{"id":7835,"name":"Nanobiotechnology","url":"https://www.academia.edu/Documents/in/Nanobiotechnology"},{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology"},{"id":17923,"name":"Autophagy","url":"https://www.academia.edu/Documents/in/Autophagy"},{"id":22050,"name":"Cytotoxicity","url":"https://www.academia.edu/Documents/in/Cytotoxicity"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":117915,"name":"Unfolded Protein Response","url":"https://www.academia.edu/Documents/in/Unfolded_Protein_Response"},{"id":122187,"name":"Endoplasmic Reticulum","url":"https://www.academia.edu/Documents/in/Endoplasmic_Reticulum"},{"id":175490,"name":"Programmed cell death","url":"https://www.academia.edu/Documents/in/Programmed_cell_death"},{"id":1335154,"name":"Propidium Iodide","url":"https://www.academia.edu/Documents/in/Propidium_Iodide"},{"id":3101476,"name":"Annexin","url":"https://www.academia.edu/Documents/in/Annexin"}],"urls":[{"id":36452227,"url":"http://link.springer.com/content/pdf/10.1186/s12951-019-0456-4.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="93323682"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/93323682/A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms"><img alt="Research paper thumbnail of A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms" class="work-thumbnail" src="https://attachments.academia-assets.com/96092610/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/93323682/A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms">A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms</a></div><div class="wp-workCard_item"><span>International Journal of Nanomedicine</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: The size of nanoparticles is considered to influence their toxicity, as smallersized ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: The size of nanoparticles is considered to influence their toxicity, as smallersized nanoparticles should more easily penetrate the cell and exert toxic effects. However, conflicting results and unstandardized methodology have resulted in controversy of these size-dependent effects. Here, we introduce a unique approach to study such size-dependent effects of nanoparticles and present evidence that reliably supports this general assumption along with elucidation of the underlying cytotoxic mechanism. Methods: We prepared and physically characterized size-controlled (20-50 nm) monodispersed silica nanoparticles (SNPs) in aqueous suspensions. Then, a variety of biochemical assessments are used for evaluating the cytotoxic mechanisms. Results: SNP treatment in three cell lines decreased cell viability and migration ability, while ROS production increased in dose-and size-dependent manners, with SNPs <30 nm showing the greatest effects. 30-and 40-nm SNPs were observed similar to these biological activities of 20-and 50-nm, respectively. Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC 50 values (75.2 and 175.2 渭g/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs. Conclusion: Our results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b0ed2afeda5321698308f2855aa81125" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96092610,"asset_id":93323682,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96092610/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="93323682"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="93323682"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 93323682; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=93323682]").text(description); $(".js-view-count[data-work-id=93323682]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 93323682; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='93323682']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 93323682, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b0ed2afeda5321698308f2855aa81125" } } $('.js-work-strip[data-work-id=93323682]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":93323682,"title":"A reliable approach for assessing size-dependent effects of silica nanoparticles on cellular internalization behavior and cytotoxic mechanisms","translated_title":"","metadata":{"publisher":"Informa UK Limited","grobid_abstract":"Background: The size of nanoparticles is considered to influence their toxicity, as smallersized nanoparticles should more easily penetrate the cell and exert toxic effects. However, conflicting results and unstandardized methodology have resulted in controversy of these size-dependent effects. Here, we introduce a unique approach to study such size-dependent effects of nanoparticles and present evidence that reliably supports this general assumption along with elucidation of the underlying cytotoxic mechanism. Methods: We prepared and physically characterized size-controlled (20-50 nm) monodispersed silica nanoparticles (SNPs) in aqueous suspensions. Then, a variety of biochemical assessments are used for evaluating the cytotoxic mechanisms. Results: SNP treatment in three cell lines decreased cell viability and migration ability, while ROS production increased in dose-and size-dependent manners, with SNPs \u003c30 nm showing the greatest effects. 30-and 40-nm SNPs were observed similar to these biological activities of 20-and 50-nm, respectively. Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC 50 values (75.2 and 175.2 渭g/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs. Conclusion: Our results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"International Journal of Nanomedicine","grobid_abstract_attachment_id":96092610},"translated_abstract":null,"internal_url":"https://www.academia.edu/93323682/A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms","translated_internal_url":"","created_at":"2022-12-20T03:59:13.835-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":96092610,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96092610/thumbnails/1.jpg","file_name":"getfile.pdf","download_url":"https://www.academia.edu/attachments/96092610/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_reliable_approach_for_assessing_size_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96092610/getfile-libre.pdf?1671540993=\u0026response-content-disposition=attachment%3B+filename%3DA_reliable_approach_for_assessing_size_d.pdf\u0026Expires=1734563128\u0026Signature=Mbe53J~ucad1r0cevCYCPawDmZE1DV5yXqsdSI3aSLoAj9GemOSTizVl-JV7Jj0qngF7ChxVaMO0HpAhNUSlFXh9b1iLzgvjrNNAnUQ~fYZMc2fxfDFvA8jeeXZgCz10kXM1Wh0-PXPcHvAgPVyjfXoWNMcSO62OSqbTQBDi-bdq3yrT4i4xlL6vy2qeuBSDoM~PLzBHmTyzXWlV43Bb4E008sdiVqckzljHvVncbJBJDE3fzWR0o5TEXEyXdcfuLK~y5I7Pzh43vcpcW1qOZYEk6a~zk9UFQ2b5-HGzBsSDYwviUng8ddzhsWv6X-bchpCrHXoxwqtnXfu8xyjJjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_reliable_approach_for_assessing_size_dependent_effects_of_silica_nanoparticles_on_cellular_internalization_behavior_and_cytotoxic_mechanisms","translated_slug":"","page_count":13,"language":"en","content_type":"Work","summary":"Background: The size of nanoparticles is considered to influence their toxicity, as smallersized nanoparticles should more easily penetrate the cell and exert toxic effects. 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Under the conventionally used serum-free conditions, both 20-nm and 50-nm SNPs at the IC 50 values (75.2 and 175.2 渭g/mL) induced apoptosis and necrosis in HepG2 cells, whereas necrosis was more rapid with the smaller SNPs. Inhibiting endocytosis impeded the internalization of the 50-nm but not the 20-nm SNPs. However, agglomeration following serum exposure increased the size of the 20-nm SNPs to approximately 50 nm, preventing their internalization and cell membrane damage without necrosis. Thus, 20-nm and 50-nm SNPs show different modes of cellular uptake, with smaller SNPs capable of trafficking into the cells in an endocytosis-independent manner. This approach of using non-overlapping size classes of SNPs under the same dose, along with serum-induced agglomeration analysis clarifies this long-standing question about the safety of small SNPs. Conclusion: Our results highlight the need to revise safety guidelines to account for this demonstrated size-dependent cytotoxicity under serum-free conditions, which may be similar to the microenvironment after tissue penetration.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":96092610,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96092610/thumbnails/1.jpg","file_name":"getfile.pdf","download_url":"https://www.academia.edu/attachments/96092610/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_reliable_approach_for_assessing_size_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96092610/getfile-libre.pdf?1671540993=\u0026response-content-disposition=attachment%3B+filename%3DA_reliable_approach_for_assessing_size_d.pdf\u0026Expires=1734563129\u0026Signature=OhVrJcyxqgc0xGfhBEF~9hNsypr9wlwD6dKmjcbAfnlIMWeL5M-SPQjdAAKTmenpHcwEW~f9uiPWEPcIB7Momn8-6549TSBAoQjqQzK-9t73fTlnbBFoBO2udGMBVcB27uVLUKYuu~46-fZTmUTPIXjYY1JrCv3bpQ7yNXdpDy5HZTdZlmzAKbLXPL5anPymzYeJs-MRgSFJa8b1s3I4yjm~ETxxm5aUXytfKdwlKYqP1pwtWA5NEmF0ejSAYoZz4sF7OeRNZ6iFufDeHY3--mU4jlKBZc7HjZ5U9EYdSsXNz7hIy0qotJbX3mMpCrVTZSmRKG5DwMQQSyGqMycEjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":96092611,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96092611/thumbnails/1.jpg","file_name":"getfile.pdf","download_url":"https://www.academia.edu/attachments/96092611/download_file","bulk_download_file_name":"A_reliable_approach_for_assessing_size_d.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96092611/getfile-libre.pdf?1671540988=\u0026response-content-disposition=attachment%3B+filename%3DA_reliable_approach_for_assessing_size_d.pdf\u0026Expires=1734563129\u0026Signature=C6wwjxVNdqDY91AAuCaYGDQXPwREujtz-yUYFyhdoTprUHqiSrpLotbmNd8hQtCxhBMK583-TrubJ4AiyJyed-~BHSYVt~ZKyb3hwRmicDCS2Iduk3rRD9PqgpnjqFSej8~7Y70yzF7skb8m2Dg6NmSmo4ZNOfZ8SLNACNCcN-~MFgvKCEdH0dmQF9QKNvR5ClL3nKSNR1Vd65MoHsxmQEgeBaQ12pYILiv4ALusPKAyoLUsDGBCfMyHObqPAofJgVof5BvpbgIKcX-t2BXxAAMsow3QdhBjGxEhG1MVRcP--VXffOKtNtTtJliJDp2XX569~6BVWI7Jt7Xazk7BgQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":11972,"name":"Nanomedicine","url":"https://www.academia.edu/Documents/in/Nanomedicine"},{"id":17733,"name":"Nanotechnology","url":"https://www.academia.edu/Documents/in/Nanotechnology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":51613,"name":"Internalization","url":"https://www.academia.edu/Documents/in/Internalization"},{"id":90156,"name":"Endocytosis","url":"https://www.academia.edu/Documents/in/Endocytosis"},{"id":1335152,"name":"Viability assay","url":"https://www.academia.edu/Documents/in/Viability_assay"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":27247258,"url":"https://www.dovepress.com/getfile.php?fileID=52672"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320694"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320694/Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles"><img alt="Research paper thumbnail of Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320694/Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles">Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles</a></div><div class="wp-workCard_item"><span>Current Pharmaceutical Design</span><span>, Mar 1, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide e...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320694"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320694"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320694; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320694]").text(description); $(".js-view-count[data-work-id=60320694]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320694; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320694']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320694, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320694]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320694,"title":"Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles","translated_title":"","metadata":{"abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.","publication_date":{"day":1,"month":3,"year":2015,"errors":{}},"publication_name":"Current Pharmaceutical Design"},"translated_abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.","internal_url":"https://www.academia.edu/60320694/Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles","translated_internal_url":"","created_at":"2021-10-29T08:02:16.270-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Molecular_Imaging_and_Targeted_Drug_Delivery_Using_Albumin_Based_Nanoparticles","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This article will summarize the developments in albumin nanocarriers for biomedical imaging and targeted drug delivery. This review will give an account of the different applications of albumin carriers with examples of some recent innovative works.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":39857,"name":"Molecular Imaging","url":"https://www.academia.edu/Documents/in/Molecular_Imaging"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":2349258,"name":"Serum albumin","url":"https://www.academia.edu/Documents/in/Serum_albumin"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":13799184,"url":"http://pubmed.cn/25732551"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320691"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320691/In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes"><img alt="Research paper thumbnail of In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320691/In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes">In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes</a></div><div class="wp-workCard_item"><span>Amino acids</span><span>, Jul 20, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-reg...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320691"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320691"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320691; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320691]").text(description); $(".js-view-count[data-work-id=60320691]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320691; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320691']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320691, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320691]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320691,"title":"In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes","translated_title":"","metadata":{"abstract":"Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...","publication_date":{"day":20,"month":7,"year":2016,"errors":{}},"publication_name":"Amino acids"},"translated_abstract":"Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...","internal_url":"https://www.academia.edu/60320691/In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes","translated_internal_url":"","created_at":"2021-10-29T08:02:14.190-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"In_vivo_monitoring_of_angiogenesis_in_a_mouse_hindlimb_ischemia_model_using_fluorescent_peptide_based_probes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-target...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":33302,"name":"Optical Imaging","url":"https://www.academia.edu/Documents/in/Optical_Imaging"},{"id":82983,"name":"Ischemia","url":"https://www.academia.edu/Documents/in/Ischemia"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":151086,"name":"Peptides","url":"https://www.academia.edu/Documents/in/Peptides"},{"id":295928,"name":"Amino Acids","url":"https://www.academia.edu/Documents/in/Amino_Acids"},{"id":3276666,"name":"fluorescent dyes","url":"https://www.academia.edu/Documents/in/fluorescent_dyes"},{"id":3789880,"name":"Medical biochemistry and metabolomics","url":"https://www.academia.edu/Documents/in/Medical_biochemistry_and_metabolomics"},{"id":3802434,"name":"Human Umbilical Vein Endothelial Cells","url":"https://www.academia.edu/Documents/in/Human_Umbilical_Vein_Endothelial_Cells"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320690"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320690/Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_"><img alt="Research paper thumbnail of Theranostics: Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications (Small 9/2016)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320690/Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_">Theranostics: Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications (Small 9/2016)</a></div><div class="wp-workCard_item"><span>Small (Weinheim an der Bergstrasse, Germany)</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320690"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320690"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320690; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320690]").text(description); $(".js-view-count[data-work-id=60320690]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320690; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320690']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320690, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320690]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320690,"title":"Theranostics: Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications (Small 9/2016)","translated_title":"","metadata":{"abstract":"Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Small (Weinheim an der Bergstrasse, Germany)"},"translated_abstract":"Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.","internal_url":"https://www.academia.edu/60320690/Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_","translated_internal_url":"","created_at":"2021-10-29T08:02:12.231-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Theranostics_Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications_Small_9_2016_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Ligand-modified, gene-loaded nanoparticles (NPs) are designed and prepared as a tumor-targeting theranostic agent by I. C. Kwon, K. Y. Lee, and co-workers. The nanoparticles offer neuroblastoma-specific in-vivo optical imaging, and adding a therapeutic gene cocktail into the NPs could play a critical role for gene-therapy-based on RNAi. On page 1201, dye-labeled NPs are modified with rabies virus glycoprotein peptide to enhance the receptor-mediated uptake by neuroblastoma, and an siRNA cocktail is loaded into the NPs, inducing RNA interference and significantly suppressing tumor growth in a mouse model.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":733610,"name":"Small","url":"https://www.academia.edu/Documents/in/Small"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320688"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320688/Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma"><img alt="Research paper thumbnail of Theranostic gas-generating nanoparticles for targeted ultrasound imaging and treatment of neuroblastoma" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320688/Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma">Theranostic gas-generating nanoparticles for targeted ultrasound imaging and treatment of neuroblastoma</a></div><div class="wp-workCard_item"><span>Journal of controlled release : official journal of the Controlled Release Society</span><span>, Jan 29, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinic...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320688"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320688"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320688; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320688]").text(description); $(".js-view-count[data-work-id=60320688]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320688; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320688']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320688, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320688]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320688,"title":"Theranostic gas-generating nanoparticles for targeted ultrasound imaging and treatment of neuroblastoma","translated_title":"","metadata":{"abstract":"The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...","publication_date":{"day":29,"month":1,"year":2015,"errors":{}},"publication_name":"Journal of controlled release : official journal of the Controlled Release Society"},"translated_abstract":"The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...","internal_url":"https://www.academia.edu/60320688/Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma","translated_internal_url":"","created_at":"2021-10-29T08:02:10.694-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Theranostic_gas_generating_nanoparticles_for_targeted_ultrasound_imaging_and_treatment_of_neuroblastoma","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The development of safe and efficient diagnostic/therapeutic agents for treating cancer in clinics remains challenging due to the potential toxicity of conventional agents. Although the annual incidence of neuroblastoma is not that high, the disease mainly occurs in children, a population vulnerable to toxic contrast agents and therapeutics. We demonstrate here that cancer-targeting, gas-generating polymeric nanoparticles are useful as a theranostic tool for ultrasound (US) imaging and treating neuroblastoma. We encapsulated calcium carbonate using poly(d,l-lactide-co-glycolide) and created gas-generating polymer nanoparticles (GNPs). These nanoparticles release carbon dioxide bubbles under acidic conditions and enhance US signals. When GNPs are modified using rabies virus glycoprotein (RVG) peptide, a targeting moiety to neuroblastoma, RVG-GNPs effectively accumulate at the tumor site and substantially enhance US signals in a tumor-bearing mouse model. Intravenous administration of...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide"},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":191439,"name":"Glycoproteins","url":"https://www.academia.edu/Documents/in/Glycoproteins"},{"id":203765,"name":"Diagnostic Imaging","url":"https://www.academia.edu/Documents/in/Diagnostic_Imaging"},{"id":256805,"name":"Neuroblastoma","url":"https://www.academia.edu/Documents/in/Neuroblastoma"},{"id":630941,"name":"Calcium Carbonate","url":"https://www.academia.edu/Documents/in/Calcium_Carbonate"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":3101595,"name":"Ultrasonic Waves","url":"https://www.academia.edu/Documents/in/Ultrasonic_Waves"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320686"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320686/The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment"><img alt="Research paper thumbnail of The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320686/The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment">The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment</a></div><div class="wp-workCard_item"><span>Nanoscale</span><span>, Jan 21, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Although chitosan and its derivatives have been frequently utilized as delivery vehicles for smal...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320686"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320686"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320686; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320686]").text(description); $(".js-view-count[data-work-id=60320686]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320686; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320686']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320686, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320686]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320686,"title":"The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment","translated_title":"","metadata":{"abstract":"Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...","publication_date":{"day":21,"month":1,"year":2015,"errors":{}},"publication_name":"Nanoscale"},"translated_abstract":"Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...","internal_url":"https://www.academia.edu/60320686/The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment","translated_internal_url":"","created_at":"2021-10-29T08:02:09.088-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_spacer_arm_length_in_cell_penetrating_peptides_influences_chitosan_siRNA_nanoparticle_delivery_for_pulmonary_inflammation_treatment","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R9Gn) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R9Gn-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R9Gn-chitosan and siRNA. However, R9G10-chitosan was much more effective in delivering genes both i...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":9130,"name":"Chitosan","url":"https://www.academia.edu/Documents/in/Chitosan"},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation"},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":29149,"name":"Extracellular Matrix","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix"},{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques"},{"id":111112,"name":"Pneumonia","url":"https://www.academia.edu/Documents/in/Pneumonia"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":555120,"name":"Arginine","url":"https://www.academia.edu/Documents/in/Arginine"},{"id":712543,"name":"Glycine","url":"https://www.academia.edu/Documents/in/Glycine"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":2468093,"name":"Cell Membrane","url":"https://www.academia.edu/Documents/in/Cell_Membrane"},{"id":2796118,"name":"nanoscale","url":"https://www.academia.edu/Documents/in/nanoscale"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320685"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320685/Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications"><img alt="Research paper thumbnail of Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320685/Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications">Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications</a></div><div class="wp-workCard_item"><span>Small (Weinheim an der Bergstrasse, Germany)</span><span>, Jan 17, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracte...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320685"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320685"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320685; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320685]").text(description); $(".js-view-count[data-work-id=60320685]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320685; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320685']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320685, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320685]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320685,"title":"Optical Imaging and Gene Therapy with Neuroblastoma-Targeting Polymeric Nanoparticles for Potential Theranostic Applications","translated_title":"","metadata":{"abstract":"Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...","publication_date":{"day":17,"month":1,"year":2015,"errors":{}},"publication_name":"Small (Weinheim an der Bergstrasse, Germany)"},"translated_abstract":"Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...","internal_url":"https://www.academia.edu/60320685/Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications","translated_internal_url":"","created_at":"2021-10-29T08:02:07.461-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Optical_Imaging_and_Gene_Therapy_with_Neuroblastoma_Targeting_Polymeric_Nanoparticles_for_Potential_Theranostic_Applications","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Recently, targeted delivery systems based on functionalized polymeric nanoparticles have attracted a great deal of attention in cancer diagnosis and therapy. Specifically, as neuroblastoma occurs in infancy and childhood, targeted delivery may be critical to reduce the side effects that can occur with conventional approaches, as well as to achieve precise diagnosis and efficient therapy. Thus, biocompatible poly(d,l-lactide-co-glycolide) (PLG) nanoparticles containing an imaging probe and therapeutic gene are prepared, followed by modification with rabies virus glycoprotein (RVG) peptide for neuroblastoma-targeting delivery. RVG peptide is a well-known neuronal targeting ligand and is chemically conjugated to PLG nanoparticles without changing their size or shape. RVG-modified nanoparticles are effective in specifically targeting neuroblastoma both in vitro and in vivo. RVG-modified nanoparticles loaded with a fluorescent probe are useful to detect the tumor site in a neuroblastoma-...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing"},{"id":33302,"name":"Optical Imaging","url":"https://www.academia.edu/Documents/in/Optical_Imaging"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":256805,"name":"Neuroblastoma","url":"https://www.academia.edu/Documents/in/Neuroblastoma"},{"id":733610,"name":"Small","url":"https://www.academia.edu/Documents/in/Small"},{"id":1010136,"name":"Rabies Virus","url":"https://www.academia.edu/Documents/in/Rabies_Virus"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":2950651,"name":"Tissue distribution","url":"https://www.academia.edu/Documents/in/Tissue_distribution"},{"id":3061075,"name":"Genetic Therapy","url":"https://www.academia.edu/Documents/in/Genetic_Therapy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320681"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320681/Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids"><img alt="Research paper thumbnail of Tuning the sphere-to-rod transition in the self-assembly of thermoresponsive polymer hybrids" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320681/Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids">Tuning the sphere-to-rod transition in the self-assembly of thermoresponsive polymer hybrids</a></div><div class="wp-workCard_item"><span>Colloids and surfaces. B, Biointerfaces</span><span>, Jan 9, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Nano-scale drug delivery systems have undergone extensive development, and control of size and st...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320681"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320681"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320681; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320681]").text(description); $(".js-view-count[data-work-id=60320681]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320681; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320681']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320681, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320681]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320681,"title":"Tuning the sphere-to-rod transition in the self-assembly of thermoresponsive polymer hybrids","translated_title":"","metadata":{"abstract":"Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...","publication_date":{"day":9,"month":1,"year":2015,"errors":{}},"publication_name":"Colloids and surfaces. B, Biointerfaces"},"translated_abstract":"Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...","internal_url":"https://www.academia.edu/60320681/Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids","translated_internal_url":"","created_at":"2021-10-29T08:02:05.966-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Tuning_the_sphere_to_rod_transition_in_the_self_assembly_of_thermoresponsive_polymer_hybrids","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Nano-scale drug delivery systems have undergone extensive development, and control of size and structure is critical for regulation of their biological responses and therapeutic efficacy. Amphiphilic polymers that form self-assembled structures in aqueous media have been investigated and used for the diagnosis and therapy of various diseases, including cancer. Here, we report the design and fabrication of thermoresponsive polymeric micelles from alginate conjugated with poly(N-isopropylacrylamide) (PNIPAAm). Alginate-PNIPAAm hybrids formed self-aggregated structures in response to temperature changes near body temperature. A structural transition from micellar spheres to rods of alginate-PNIPAAm hybrids was observed depending on the molecular weight of PNIPAAm and the polymer concentration. Additionally, hydrogels with nanofibrous structures were formed by simply increasing the polymer concentration. This approach to controlling the structure of polymer micelles from nanoparticles t...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers"},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320680"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320680/Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles"><img alt="Research paper thumbnail of Molecular Imaging and Targeted Drug Delivery using Albumin-Based Nanoparticles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320680/Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles">Molecular Imaging and Targeted Drug Delivery using Albumin-Based Nanoparticles</a></div><div class="wp-workCard_item"><span>Current pharmaceutical design</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide e...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320680"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320680"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320680; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320680]").text(description); $(".js-view-count[data-work-id=60320680]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320680; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320680']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320680, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320680]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320680,"title":"Molecular Imaging and Targeted Drug Delivery using Albumin-Based Nanoparticles","translated_title":"","metadata":{"abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"Current pharmaceutical design"},"translated_abstract":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...","internal_url":"https://www.academia.edu/60320680/Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles","translated_internal_url":"","created_at":"2021-10-29T08:02:03.450-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Molecular_Imaging_and_Targeted_Drug_Delivery_using_Albumin_Based_Nanoparticles","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Albumin has been used as a popular material for carrying imaging probes and/or drugs to provide efficient biomedical imaging and therapy. It is considered as an ideal material for in vivo applications, because albumin is naturally abundant in serum to show non-toxicity and non-immunogenicity. In addition, based on the convenience of chemical modifications, it is widely used for the delivery of diverse molecules including chemicals, proteins/peptides, and oligonucleotides. Albumin nanoparticles carrying these molecules have shown improved pharmacokinetic properties by providing longer circulation time and more disease-specific accumulation, and they are emerging as a promising carrier system for in vivo imaging and therapy. Constant efforts to improve the properties of albumin nanoparticles have led to a great progress in medical application, and recent examples of market approval and success are brightening the prospect of albumin-based nanocarrier formulations in the future. This a...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":13621,"name":"Nanoparticles","url":"https://www.academia.edu/Documents/in/Nanoparticles"},{"id":39857,"name":"Molecular Imaging","url":"https://www.academia.edu/Documents/in/Molecular_Imaging"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":2349258,"name":"Serum albumin","url":"https://www.academia.edu/Documents/in/Serum_albumin"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320678"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320678/Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy"><img alt="Research paper thumbnail of Doxorubicin-Loaded Alginate-g-Poly(N-isopropylacrylamide) Micelles for Cancer Imaging and Therapy" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320678/Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy">Doxorubicin-Loaded Alginate-g-Poly(N-isopropylacrylamide) Micelles for Cancer Imaging and Therapy</a></div><div class="wp-workCard_item"><span>ACS applied materials & interfaces</span><span>, Jan 24, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often li...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320678"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320678"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320678; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320678]").text(description); $(".js-view-count[data-work-id=60320678]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320678; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320678']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320678, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320678]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320678,"title":"Doxorubicin-Loaded Alginate-g-Poly(N-isopropylacrylamide) Micelles for Cancer Imaging and Therapy","translated_title":"","metadata":{"abstract":"Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...","publication_date":{"day":24,"month":1,"year":2014,"errors":{}},"publication_name":"ACS applied materials \u0026 interfaces"},"translated_abstract":"Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...","internal_url":"https://www.academia.edu/60320678/Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy","translated_internal_url":"","created_at":"2021-10-29T08:02:00.371-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Doxorubicin_Loaded_Alginate_g_Poly_N_isopropylacrylamide_Micelles_for_Cancer_Imaging_and_Therapy","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Chemotherapy is a widely adopted method for the treatment of cancer. However, its use is often limited due to side effects produced by anti-cancer drugs. Therefore, various drug carriers, including polymeric micelles, have been investigated to find a method to overcome this limitation. In this study, alginate-based, self-assembled polymeric micelles were designed and prepared using alginate-g-poly(N-isopropylacrylamide) (PNIPAAm). Amino-PNIPAAm was chemically introduced to the alginate backbone via carbodiimide chemistry. The resulting polymer was dissolved in distilled water at room temperature and formed self-assembled micelles at 37 掳C. Characteristics of alginate-g-PNIPAAm micelles were dependent on the molecular weight of PNIPAAm, the degree of substitution, and the polymer concentration. Doxorubicin (DOX), a model anti-cancer drug, was efficiently encapsulated in alginate-g-PNIPAAm micelles, and sustained release of DOX from the micelles was achieved at 37 掳C in vitro. These m...","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles"},{"id":83315,"name":"Diffusion","url":"https://www.academia.edu/Documents/in/Diffusion"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":300178,"name":"Nanocapsules","url":"https://www.academia.edu/Documents/in/Nanocapsules"},{"id":314240,"name":"Doxorubicin","url":"https://www.academia.edu/Documents/in/Doxorubicin"},{"id":315194,"name":"Oral Squamous Cell Carcinoma (OSCC)","url":"https://www.academia.edu/Documents/in/Oral_Squamous_Cell_Carcinoma_OSCC_"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":604754,"name":"Acrylic Resins","url":"https://www.academia.edu/Documents/in/Acrylic_Resins"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320676"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320676/Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis"><img alt="Research paper thumbnail of Co-delivery of Vascular Endothelial Growth Factor and Angiopoietin-1 Using Injectable Microsphere/Hydrogel Hybrid Systems for Therapeutic Angiogenesis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320676/Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis">Co-delivery of Vascular Endothelial Growth Factor and Angiopoietin-1 Using Injectable Microsphere/Hydrogel Hybrid Systems for Therapeutic Angiogenesis</a></div><div class="wp-workCard_item"><span>Pharmaceutical Research</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoie...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320676"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320676"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320676; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320676]").text(description); $(".js-view-count[data-work-id=60320676]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320676; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320676']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320676, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320676]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320676,"title":"Co-delivery of Vascular Endothelial Growth Factor and Angiopoietin-1 Using Injectable Microsphere/Hydrogel Hybrid Systems for Therapeutic Angiogenesis","translated_title":"","metadata":{"abstract":"We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.","publisher":"Springer Science and Business Media LLC","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Pharmaceutical Research"},"translated_abstract":"We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.","internal_url":"https://www.academia.edu/60320676/Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis","translated_internal_url":"","created_at":"2021-10-29T08:01:57.479-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Co_delivery_of_Vascular_Endothelial_Growth_Factor_and_Angiopoietin_1_Using_Injectable_Microsphere_Hydrogel_Hybrid_Systems_for_Therapeutic_Angiogenesis","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"We hypothesized that combined delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) using microsphere/hydrogel hybrid systems could enhance mature vessel formation compared with administration of each factor alone. Hybrid delivery systems composed of alginate hydrogels and poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres containing angiogenic factors were prepared. The release behavior of angiogenic factors from hybrid systems was monitored in vitro. The hybrid systems were injected into an ischemic rodent model, and blood vessel formation at the ischemic site was evaluated. The sustained release over 4 weeks of both VEGF and Ang-1 from hybrid systems was achieved in vitro. Co-delivery of VEGF and Ang-1 was advantageous to retain muscle tissues and significantly induced vessel enlargement at the ischemic site, compared to mice treated with either VEGF or Ang-1 alone. Sustained and combined delivery of VEGF and Ang-1 significantly enhances vessel enlargement at the ischemic site, compared with sustained delivery of either factor alone. Microsphere/hydrogel hybrid systems may be a promising vehicle for delivery of multiple drugs for many therapeutic applications.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical"},{"id":102727,"name":"Hydrogel","url":"https://www.academia.edu/Documents/in/Hydrogel"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1137107,"name":"Delayed-Action Preparations","url":"https://www.academia.edu/Documents/in/Delayed-Action_Preparations"},{"id":2647749,"name":"angiopoietin","url":"https://www.academia.edu/Documents/in/angiopoietin"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320673"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320673/Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System"><img alt="Research paper thumbnail of Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System" class="work-thumbnail" src="https://attachments.academia-assets.com/73819611/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320673/Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System">Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System</a></div><div class="wp-workCard_item"><span>Pharmaceutical Research</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. Methods. A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated. Results. The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system. Conclusion. A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="10a98f5ea9624572ef90f46b36d2decd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819611,"asset_id":60320673,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819611/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320673"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320673"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320673; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320673]").text(description); $(".js-view-count[data-work-id=60320673]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320673; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320673']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320673, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "10a98f5ea9624572ef90f46b36d2decd" } } $('.js-work-strip[data-work-id=60320673]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320673,"title":"Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System","translated_title":"","metadata":{"publisher":"Springer Nature","grobid_abstract":"Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. Methods. A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated. Results. The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system. Conclusion. A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Pharmaceutical Research","grobid_abstract_attachment_id":73819611},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320673/Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System","translated_internal_url":"","created_at":"2021-10-29T08:01:54.657-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819611,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819611/thumbnails/1.jpg","file_name":"s11095-010-0067-020211029-14358-ov94fs.pdf","download_url":"https://www.academia.edu/attachments/73819611/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Active_Blood_Vessel_Formation_in_the_Isc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819611/s11095-010-0067-020211029-14358-ov94fs-libre.pdf?1635521172=\u0026response-content-disposition=attachment%3B+filename%3DActive_Blood_Vessel_Formation_in_the_Isc.pdf\u0026Expires=1734563129\u0026Signature=H-aVxS~M8hb5gKS4gXXph7n0X7BihVIJ7pXrldZlV8QrWq9eCPVoNjbUoGDQJ8fnEdYf~P0io~kpozHcbVMn9T6RldA~UeLk86ilDqMfNEnf8VWswqELrzhGG8QLUhBdRbGYWx6H4MfT7crdw5MhX6EdPbG5CA2LmmJ2aTBzvP4hacigjl436XODDLelu2w0r4ZL8xcE9aa59t8Y2SEyO2WuPbQy-F4-UFjR4RdGw2CggvE117w4jRvlqEAVAVnMDxDk1jWw6je4sG1nMM3rlMpCh3Nbovx0ouUdyb4c2ydvLswYwnjZd4hpMCFaEFtDXST1hrR8Zhe7lpVFK~jApg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Active_Blood_Vessel_Formation_in_the_Ischemic_Hindlimb_Mouse_Model_Using_a_Microsphere_Hydrogel_Combination_System","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"Purpose. We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations. Methods. A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated. Results. The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system. Conclusion. A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819611,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819611/thumbnails/1.jpg","file_name":"s11095-010-0067-020211029-14358-ov94fs.pdf","download_url":"https://www.academia.edu/attachments/73819611/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Active_Blood_Vessel_Formation_in_the_Isc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819611/s11095-010-0067-020211029-14358-ov94fs-libre.pdf?1635521172=\u0026response-content-disposition=attachment%3B+filename%3DActive_Blood_Vessel_Formation_in_the_Isc.pdf\u0026Expires=1734563129\u0026Signature=H-aVxS~M8hb5gKS4gXXph7n0X7BihVIJ7pXrldZlV8QrWq9eCPVoNjbUoGDQJ8fnEdYf~P0io~kpozHcbVMn9T6RldA~UeLk86ilDqMfNEnf8VWswqELrzhGG8QLUhBdRbGYWx6H4MfT7crdw5MhX6EdPbG5CA2LmmJ2aTBzvP4hacigjl436XODDLelu2w0r4ZL8xcE9aa59t8Y2SEyO2WuPbQy-F4-UFjR4RdGw2CggvE117w4jRvlqEAVAVnMDxDk1jWw6je4sG1nMM3rlMpCh3Nbovx0ouUdyb4c2ydvLswYwnjZd4hpMCFaEFtDXST1hrR8Zhe7lpVFK~jApg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry"},{"id":22442,"name":"Hydrogels","url":"https://www.academia.edu/Documents/in/Hydrogels"},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":82983,"name":"Ischemia","url":"https://www.academia.edu/Documents/in/Ischemia"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1135766,"name":"Excipients","url":"https://www.academia.edu/Documents/in/Excipients"},{"id":1137107,"name":"Delayed-Action Preparations","url":"https://www.academia.edu/Documents/in/Delayed-Action_Preparations"},{"id":1796877,"name":"Vascular Endothelial Growth Factor","url":"https://www.academia.edu/Documents/in/Vascular_Endothelial_Growth_Factor"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320672"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320672/Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System"><img alt="Research paper thumbnail of Local and Sustained Vascular Endothelial Growth Factor Delivery for Angiogenesis Using an Injectable System" class="work-thumbnail" src="https://attachments.academia-assets.com/73819692/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320672/Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System">Local and Sustained Vascular Endothelial Growth Factor Delivery for Angiogenesis Using an Injectable System</a></div><div class="wp-workCard_item"><span>Pharmaceutical Research</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the lo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the local and sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis in vivo. Methods. Poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing rhVEGF were loaded into alginate gels by ionic cross-linking. The rhVEGF release from the system was monitored and bioactivity was tested in vitro. The combination system was subcutaneously injected into mice using a syringe, and new blood vessel formation was evaluated. Results. Sustained rhVEGF release from the combination system was observed for 3 weeks, and the released rhVEGF remained bioactive. Endothelial cell proliferation was significantly enhanced when cells were cultured with the rhVEGF-releasing combination system in vitro. When the combination system was implanted, the granulation tissue layer was thicker with more newly formed blood vessels than that with a single dose VEGF injection. Conclusion. The rhVEGF release was controlled by varying relative portions of microspheres and hydrogels in combination delivery systems, which efficiently promoted new blood vessel formation in vivo. This combination system could be a promising delivery vehicle for therapeutic angiogenesis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="770cf83025e1b28c1772e1a829364400" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819692,"asset_id":60320672,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819692/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320672"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320672"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320672; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320672]").text(description); $(".js-view-count[data-work-id=60320672]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320672; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320672']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320672, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "770cf83025e1b28c1772e1a829364400" } } $('.js-work-strip[data-work-id=60320672]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320672,"title":"Local and Sustained Vascular Endothelial Growth Factor Delivery for Angiogenesis Using an Injectable System","translated_title":"","metadata":{"publisher":"Springer Nature","grobid_abstract":"Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the local and sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis in vivo. Methods. Poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing rhVEGF were loaded into alginate gels by ionic cross-linking. The rhVEGF release from the system was monitored and bioactivity was tested in vitro. The combination system was subcutaneously injected into mice using a syringe, and new blood vessel formation was evaluated. Results. Sustained rhVEGF release from the combination system was observed for 3 weeks, and the released rhVEGF remained bioactive. Endothelial cell proliferation was significantly enhanced when cells were cultured with the rhVEGF-releasing combination system in vitro. When the combination system was implanted, the granulation tissue layer was thicker with more newly formed blood vessels than that with a single dose VEGF injection. Conclusion. The rhVEGF release was controlled by varying relative portions of microspheres and hydrogels in combination delivery systems, which efficiently promoted new blood vessel formation in vivo. This combination system could be a promising delivery vehicle for therapeutic angiogenesis.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}},"publication_name":"Pharmaceutical Research","grobid_abstract_attachment_id":73819692},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320672/Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System","translated_internal_url":"","created_at":"2021-10-29T08:01:52.591-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819692,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819692/thumbnails/1.jpg","file_name":"s11095-009-9884-420211029-14352-gt5w09.pdf","download_url":"https://www.academia.edu/attachments/73819692/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Local_and_Sustained_Vascular_Endothelial.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819692/s11095-009-9884-420211029-14352-gt5w09-libre.pdf?1635521168=\u0026response-content-disposition=attachment%3B+filename%3DLocal_and_Sustained_Vascular_Endothelial.pdf\u0026Expires=1734563129\u0026Signature=WK-YiBV3C1M5m3AHbXJ7Sw6QS~7NTF2d2NEY~8pwv3bgy0a2KSz5vHKkH~xBHj0U8Yu8Z6PrLYShvoM7SKMzugQ70bcxePzYLigPhX8JlbYEvFNLXAnPntD1qgUK2E9GGBEeIEcvhR8XrTnK5oIHEVNyrChRGb9WZbfAHoz5QAGpe6xBM21GWAJPO5U0BvOYMYCyvrKNF1LY569JwjgK2GVaCARmnK2Gh0rRdLAr8C21n19v9f4-5JThaE1--X9DXyP3ptX5pTUtkg6Sd4tnwHhxHxioyuhZkgNHx~OOi8LvoiCLjSpBtkeC1preeGUGUjhFASIUOiUdekpPlmjYqw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Local_and_Sustained_Vascular_Endothelial_Growth_Factor_Delivery_for_Angiogenesis_Using_an_Injectable_System","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Purpose. We hypothesize that a microsphere/hydrogel combination system could be useful for the local and sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis in vivo. Methods. Poly(d,l-lactide-co-glycolide) (PLGA) microspheres containing rhVEGF were loaded into alginate gels by ionic cross-linking. The rhVEGF release from the system was monitored and bioactivity was tested in vitro. The combination system was subcutaneously injected into mice using a syringe, and new blood vessel formation was evaluated. Results. Sustained rhVEGF release from the combination system was observed for 3 weeks, and the released rhVEGF remained bioactive. Endothelial cell proliferation was significantly enhanced when cells were cultured with the rhVEGF-releasing combination system in vitro. When the combination system was implanted, the granulation tissue layer was thicker with more newly formed blood vessels than that with a single dose VEGF injection. Conclusion. The rhVEGF release was controlled by varying relative portions of microspheres and hydrogels in combination delivery systems, which efficiently promoted new blood vessel formation in vivo. This combination system could be a promising delivery vehicle for therapeutic angiogenesis.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819692,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819692/thumbnails/1.jpg","file_name":"s11095-009-9884-420211029-14352-gt5w09.pdf","download_url":"https://www.academia.edu/attachments/73819692/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Local_and_Sustained_Vascular_Endothelial.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819692/s11095-009-9884-420211029-14352-gt5w09-libre.pdf?1635521168=\u0026response-content-disposition=attachment%3B+filename%3DLocal_and_Sustained_Vascular_Endothelial.pdf\u0026Expires=1734563129\u0026Signature=WK-YiBV3C1M5m3AHbXJ7Sw6QS~7NTF2d2NEY~8pwv3bgy0a2KSz5vHKkH~xBHj0U8Yu8Z6PrLYShvoM7SKMzugQ70bcxePzYLigPhX8JlbYEvFNLXAnPntD1qgUK2E9GGBEeIEcvhR8XrTnK5oIHEVNyrChRGb9WZbfAHoz5QAGpe6xBM21GWAJPO5U0BvOYMYCyvrKNF1LY569JwjgK2GVaCARmnK2Gh0rRdLAr8C21n19v9f4-5JThaE1--X9DXyP3ptX5pTUtkg6Sd4tnwHhxHxioyuhZkgNHx~OOi8LvoiCLjSpBtkeC1preeGUGUjhFASIUOiUdekpPlmjYqw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22442,"name":"Hydrogels","url":"https://www.academia.edu/Documents/in/Hydrogels"},{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical"},{"id":159187,"name":"Drug Delivery Systems","url":"https://www.academia.edu/Documents/in/Drug_Delivery_Systems"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1796877,"name":"Vascular Endothelial Growth Factor","url":"https://www.academia.edu/Documents/in/Vascular_Endothelial_Growth_Factor"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); 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By preparing PLGA microspheres containing the model protein and combining them with alginate gels, the study hypothesizes that the mixing ratios of these components can control protein release. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320669"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320669/Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model"><img alt="Research paper thumbnail of Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320669/Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model">Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model</a></div><div class="wp-workCard_item"><span>Journal of Drug Targeting</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury an...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320669"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320669"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320669; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320669]").text(description); $(".js-view-count[data-work-id=60320669]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320669; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320669']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320669, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320669]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320669,"title":"Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model","translated_title":"","metadata":{"abstract":"Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.","publisher":"Informa UK Limited","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Journal of Drug Targeting"},"translated_abstract":"Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.","internal_url":"https://www.academia.edu/60320669/Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model","translated_internal_url":"","created_at":"2021-10-29T08:01:49.351-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Injectable_microsphere_hydrogel_hybrid_system_containing_heat_shock_protein_as_therapy_in_a_murine_myocardial_infarction_model","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":22442,"name":"Hydrogels","url":"https://www.academia.edu/Documents/in/Hydrogels"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats"},{"id":378016,"name":"Myocardial Infarction","url":"https://www.academia.edu/Documents/in/Myocardial_Infarction"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":765872,"name":"Heat Shock Protein","url":"https://www.academia.edu/Documents/in/Heat_Shock_Protein"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1015099,"name":"Drug Targeting","url":"https://www.academia.edu/Documents/in/Drug_Targeting"},{"id":1031068,"name":"Drug Carriers","url":"https://www.academia.edu/Documents/in/Drug_Carriers"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1137107,"name":"Delayed-Action Preparations","url":"https://www.academia.edu/Documents/in/Delayed-Action_Preparations"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320668"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320668/Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction"><img alt="Research paper thumbnail of Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction" class="work-thumbnail" src="https://attachments.academia-assets.com/73819679/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320668/Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction">Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock pro...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. In this study, in order to enhance the penetration of heat shock protein 27 (HSP27) across cell membranes, we fused HSP27 with transcriptional activator (TAT) derived from human immunodeficiency virus (HIV) as a protein transduction domain (PTD). We loaded the fusion protein (TAT-HSP27) into microsphere/hydrogel combination delivery systems to control the release behavior for prolonged time periods. We found that the release behavior of TAT-HSP27 was able to be controlled by varying the ratio of PLGA microspheres and alginate hydrogels. Indeed, the released fusion protein maintained its bioactivity and could recover the proliferation of cardiomyoblasts cultured under hypoxic conditions. This approach to controlling the release behavior of TAT-HSP27 using microsphere/hydrogel combination delivery systems may be useful for treating myocardial infarction in a minimally invasive manner.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5e0e3c83a63d5b877b36592715040756" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819679,"asset_id":60320668,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819679/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320668"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320668"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320668; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320668]").text(description); $(".js-view-count[data-work-id=60320668]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320668; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320668']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320668, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5e0e3c83a63d5b877b36592715040756" } } $('.js-work-strip[data-work-id=60320668]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320668,"title":"Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. In this study, in order to enhance the penetration of heat shock protein 27 (HSP27) across cell membranes, we fused HSP27 with transcriptional activator (TAT) derived from human immunodeficiency virus (HIV) as a protein transduction domain (PTD). We loaded the fusion protein (TAT-HSP27) into microsphere/hydrogel combination delivery systems to control the release behavior for prolonged time periods. We found that the release behavior of TAT-HSP27 was able to be controlled by varying the ratio of PLGA microspheres and alginate hydrogels. Indeed, the released fusion protein maintained its bioactivity and could recover the proliferation of cardiomyoblasts cultured under hypoxic conditions. This approach to controlling the release behavior of TAT-HSP27 using microsphere/hydrogel combination delivery systems may be useful for treating myocardial infarction in a minimally invasive manner.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}},"publication_name":"Journal of Controlled Release","grobid_abstract_attachment_id":73819679},"translated_abstract":null,"internal_url":"https://www.academia.edu/60320668/Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction","translated_internal_url":"","created_at":"2021-10-29T08:01:46.845-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":73819679,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819679/thumbnails/1.jpg","file_name":"j.jconrel.2009.04.00820211029-14352-1pwc6he.pdf","download_url":"https://www.academia.edu/attachments/73819679/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Controlled_delivery_of_heat_shock_protei.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819679/j.jconrel.2009.04.00820211029-14352-1pwc6he-libre.pdf?1635521169=\u0026response-content-disposition=attachment%3B+filename%3DControlled_delivery_of_heat_shock_protei.pdf\u0026Expires=1734563129\u0026Signature=OIPL3g9bikrQP726tKEDmHlSwjeGevMCwaVo2Sv8CSRWroME5KEvuY4WTcfinxc4ywRd015uru6~Sjp4Z~Yw8~opow4IlX3Yn5incdO1J-~SALDe4dvKtyTZ3e2VuOYWvUpcr4S0xYdc6HZedt~EXh0J9Q4rTQpRBFSm7QlLZladORHroUAGsIV8vDhvq~pLVjzt529NEeA49lhNc1JRUtaFdBrovfCaF6U7bZbotgZTrlj6W54x1O6uyV8S2Yg8K9Ez0Jl8P7ruXSceuElWFw13BHSw0kcjXRMtuPZcEsFmdN70HKaVWZFk5u6-A-8k9-NYEh0KCpmhWQbQ51d9GQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Controlled_delivery_of_heat_shock_protein_using_an_injectable_microsphere_hydrogel_combination_system_for_the_treatment_of_myocardial_infarction","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":"Myocardial infarction causes a high rate of morbidity and mortality worldwide, and heat shock proteins as molecular chaperones have been attractive targets for protecting cardiomyoblasts under environmental stimuli. 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This approach to controlling the release behavior of TAT-HSP27 using microsphere/hydrogel combination delivery systems may be useful for treating myocardial infarction in a minimally invasive manner.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[{"id":73819679,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/73819679/thumbnails/1.jpg","file_name":"j.jconrel.2009.04.00820211029-14352-1pwc6he.pdf","download_url":"https://www.academia.edu/attachments/73819679/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Controlled_delivery_of_heat_shock_protei.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/73819679/j.jconrel.2009.04.00820211029-14352-1pwc6he-libre.pdf?1635521169=\u0026response-content-disposition=attachment%3B+filename%3DControlled_delivery_of_heat_shock_protei.pdf\u0026Expires=1734563129\u0026Signature=OIPL3g9bikrQP726tKEDmHlSwjeGevMCwaVo2Sv8CSRWroME5KEvuY4WTcfinxc4ywRd015uru6~Sjp4Z~Yw8~opow4IlX3Yn5incdO1J-~SALDe4dvKtyTZ3e2VuOYWvUpcr4S0xYdc6HZedt~EXh0J9Q4rTQpRBFSm7QlLZladORHroUAGsIV8vDhvq~pLVjzt529NEeA49lhNc1JRUtaFdBrovfCaF6U7bZbotgZTrlj6W54x1O6uyV8S2Yg8K9Ez0Jl8P7ruXSceuElWFw13BHSw0kcjXRMtuPZcEsFmdN70HKaVWZFk5u6-A-8k9-NYEh0KCpmhWQbQ51d9GQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV"},{"id":41747,"name":"Dosage Form","url":"https://www.academia.edu/Documents/in/Dosage_Form"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":102727,"name":"Hydrogel","url":"https://www.academia.edu/Documents/in/Hydrogel"},{"id":318308,"name":"Human immunodeficiency virus","url":"https://www.academia.edu/Documents/in/Human_immunodeficiency_virus"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates"},{"id":421276,"name":"Delivery System","url":"https://www.academia.edu/Documents/in/Delivery_System"},{"id":556132,"name":"Microsphere","url":"https://www.academia.edu/Documents/in/Microsphere"},{"id":575076,"name":"Heat Shock","url":"https://www.academia.edu/Documents/in/Heat_Shock"},{"id":765872,"name":"Heat Shock Protein","url":"https://www.academia.edu/Documents/in/Heat_Shock_Protein"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":1159037,"name":"Fusion Protein","url":"https://www.academia.edu/Documents/in/Fusion_Protein"},{"id":2468093,"name":"Cell Membrane","url":"https://www.academia.edu/Documents/in/Cell_Membrane"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320666"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320666/Facile_control_of_porous_structures_of_polymer_microspheres_using_an_osmotic_agent_for_pulmonary_delivery"><img alt="Research paper thumbnail of Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery" class="work-thumbnail" src="https://attachments.academia-assets.com/73819675/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320666/Facile_control_of_porous_structures_of_polymer_microspheres_using_an_osmotic_agent_for_pulmonary_delivery">Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">It has been challenging to prepare polymeric microspheres with controlled porous structures for m...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">It has been challenging to prepare polymeric microspheres with controlled porous structures for many biomedical applications, particularly for pulmonary drug delivery. Here, we report the use of bovine serum albumin (BSA) as an osmotic agent in order to control the porous structure of poly(D,L-lactide-co-glycolide) (PLGA) microspheres prepared by a double emulsion method. BSA was useful to induce osmosis between internal and external water phases during the double emulsion process, resulting in the fabrication of microspheres with controllable, uniform porous structures. The pore size of PLGA microspheres was controlled independently from the particle size by this approach. The use of BSA as an osmotic agent reduced the initial burst of model proteins (e.g., insulin and VEGF) entrapped in the porous microspheres, and the sustained release of VEGF was achieved for two weeks in vitro. This approach to controlling porous structures of polymer microspheres could be useful to develop novel pulmonary drug delivery systems.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="29b9d99bb0b6d35fe48e358d1c17fac4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":73819675,"asset_id":60320666,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/73819675/download_file?st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&st=MTczNDU1OTUyOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320666"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320666"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320666; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320666]").text(description); $(".js-view-count[data-work-id=60320666]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320666; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320666']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320666, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "29b9d99bb0b6d35fe48e358d1c17fac4" } } $('.js-work-strip[data-work-id=60320666]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320666,"title":"Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"It has been challenging to prepare polymeric microspheres with controlled porous structures for many biomedical applications, particularly for pulmonary drug delivery. Here, we report the use of bovine serum albumin (BSA) as an osmotic agent in order to control the porous structure of poly(D,L-lactide-co-glycolide) (PLGA) microspheres prepared by a double emulsion method. BSA was useful to induce osmosis between internal and external water phases during the double emulsion process, resulting in the fabrication of microspheres with controllable, uniform porous structures. The pore size of PLGA microspheres was controlled independently from the particle size by this approach. The use of BSA as an osmotic agent reduced the initial burst of model proteins (e.g., insulin and VEGF) entrapped in the porous microspheres, and the sustained release of VEGF was achieved for two weeks in vitro. 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Howev...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320663"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320663"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320663; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=60320663]").text(description); $(".js-view-count[data-work-id=60320663]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 60320663; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='60320663']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 60320663, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=60320663]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":60320663,"title":"Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model","translated_title":"","metadata":{"abstract":"Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2011,"errors":{}},"publication_name":"Journal of Controlled Release"},"translated_abstract":"Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.","internal_url":"https://www.academia.edu/60320663/Preparation_of_budesonide_loaded_porous_PLGA_microparticles_and_their_therapeutic_efficacy_in_a_murine_asthma_model","translated_internal_url":"","created_at":"2021-10-29T08:01:44.351-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":132857751,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Preparation_of_budesonide_loaded_porous_PLGA_microparticles_and_their_therapeutic_efficacy_in_a_murine_asthma_model","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the sustained delivery of corticosteroid and a reduced number of doses could be achieved using porous polymeric microparticles. Porous microparticles were prepared from poly(lactic-co-glycolic acid) (PLGA) by a water-in-oil-in-water double emulsion method with ammonium bicarbonate as the porogen. Varying the porogen concentration controlled the morphology, particle size, and pore size of the PLGA microparticles, with particle size and pore size increasing as the porogen concentration increased. The BD loading efficiency in the porous PLGA microparticles was about 60%, and BD was released from the porous microparticles in a sustained manner for 24h in vitro. Lung uptake efficiency of the porous PLGA microparticles in mice was significantly higher than that of non-porous PLGA microparticles. Budesonide-loaded porous PLGA microparticles were delivered to asthmatic mice, and the numbers of inflammatory cells in bronchoalveolar lavage (BAL) fluid and tissue sections were significantly reduced when the drug was administrated every 3days. We also found significantly reduced bronchial hyperresponsiveness of asthmatic mice after treatment with budesonide-loaded porous PLGA microparticles. This approach to controlling the porous structure of polymeric microparticles, as well as the release behavior of drugs from the microparticles, could have useful applications in the pulmonary delivery of many therapeutic drugs.","owner":{"id":132857751,"first_name":"Jangwook","middle_initials":null,"last_name":"Lee","page_name":"JangwookLee6","domain_name":"independent","created_at":"2019-10-27T19:12:15.000-07:00","display_name":"Jangwook Lee","url":"https://independent.academia.edu/JangwookLee6"},"attachments":[],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"},{"id":8942,"name":"Treatment","url":"https://www.academia.edu/Documents/in/Treatment"},{"id":9968,"name":"Asthma","url":"https://www.academia.edu/Documents/in/Asthma"},{"id":64660,"name":"Controlled release","url":"https://www.academia.edu/Documents/in/Controlled_release"},{"id":68315,"name":"Porosity","url":"https://www.academia.edu/Documents/in/Porosity"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung"},{"id":219927,"name":"Efficiency","url":"https://www.academia.edu/Documents/in/Efficiency"},{"id":390245,"name":"Particle Size","url":"https://www.academia.edu/Documents/in/Particle_Size"},{"id":698785,"name":"Side Effect","url":"https://www.academia.edu/Documents/in/Side_Effect"},{"id":794074,"name":"Microspheres","url":"https://www.academia.edu/Documents/in/Microspheres"},{"id":1031068,"name":"Drug Carriers","url":"https://www.academia.edu/Documents/in/Drug_Carriers"},{"id":1074508,"name":"Lactic Acid","url":"https://www.academia.edu/Documents/in/Lactic_Acid"},{"id":1232391,"name":"Budesonide","url":"https://www.academia.edu/Documents/in/Budesonide"},{"id":1291661,"name":"Copolymer","url":"https://www.academia.edu/Documents/in/Copolymer"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"},{"id":4040940,"name":"Bicarbonates","url":"https://www.academia.edu/Documents/in/Bicarbonates"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="60320661"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/60320661/Single_chain_variable_fragment_CD7_antibody_conjugated_PLGA_HDAC_inhibitor_immuno_nanoparticles_Developing_human_T_cell_specific_nano_technology_for_delivery_of_therapeutic_drugs_targeting_latent_HIV"><img alt="Research paper thumbnail of Single chain variable fragment CD7 antibody conjugated PLGA/HDAC inhibitor immuno-nanoparticles: Developing human T cell-specific nano-technology for delivery of therapeutic drugs targeting latent HIV" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/60320661/Single_chain_variable_fragment_CD7_antibody_conjugated_PLGA_HDAC_inhibitor_immuno_nanoparticles_Developing_human_T_cell_specific_nano_technology_for_delivery_of_therapeutic_drugs_targeting_latent_HIV">Single chain variable fragment CD7 antibody conjugated PLGA/HDAC inhibitor immuno-nanoparticles: Developing human T cell-specific nano-technology for delivery of therapeutic drugs targeting latent HIV</a></div><div class="wp-workCard_item"><span>Journal of Controlled Release</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="60320661"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="60320661"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 60320661; 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