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diagnosis</title> <meta name="description" content="Search results for: molecular diagnosis"> <meta name="keywords" content="molecular diagnosis"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="molecular diagnosis"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4030</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: molecular diagnosis</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4030</span> The Contribution of the PCR-Enzymatic Digestion in the Positive Diagnosis of Proximal Spinal Muscular Atrophy in the Moroccan Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20Merhni">H. Merhni</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Sbiti"> A. Sbiti</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Ratbi"> I. Ratbi</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Sefiani"> A. Sefiani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The proximal spinal muscular atrophy (SMA) is a group of neuromuscular disorders characterized by progressive muscle weakness due to the degeneration and loss of anterior motor neurons of the spinal cord. Depending on the age of onset of symptoms and their evolution, four types of SMA, varying in severity, result in a mutations of the <em>SMN</em> gene (survival of Motor neuron). We have analyzed the DNA of 295 patients referred to our genetic counseling; since January 1996 until October 2014; for suspected SMA. The homozygous deletion of exon 7 of the <em>SMN</em> gene was found in 133 patients; of which, 40.6% were born to consanguineous parents. In countries like Morocco, where the frequency of heterozygotes for SMA is high, genetic testing should be offered as first-line and, after careful clinical assessment, especially in newborns and infants with congenital hypotonia unexplained and prognosis compromise. The molecular diagnosis of SMA allows a quick and certainly diagnosis, provide adequate genetic counseling for families at risk and suggest, for couples who want prenatal diagnosis. The analysis of the <em>SMN</em> gene is a perfect example of genetic testing with an excellent cost/benefit ratio that can be of great interest in public health, especially in low-income countries. We emphasize in this work for the benefit of the generalization of molecular diagnosis of SMA by the technique of PCR-enzymatic digestion in other centers in Morocco. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Exon7" title="Exon7">Exon7</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR-digestion" title=" PCR-digestion"> PCR-digestion</a>, <a href="https://publications.waset.org/abstracts/search?q=SMA" title=" SMA"> SMA</a>, <a href="https://publications.waset.org/abstracts/search?q=SMN%20gene" title=" SMN gene"> SMN gene</a> </p> <a href="https://publications.waset.org/abstracts/60592/the-contribution-of-the-pcr-enzymatic-digestion-in-the-positive-diagnosis-of-proximal-spinal-muscular-atrophy-in-the-moroccan-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60592.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">241</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4029</span> Molecular Characterization and Phylogenetic Analysis of Influenza a(H3N2) Virus Circulating during the 2010-2011 in Riyadh, Saudi Arabia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ghazanfar%20Ali">Ghazanfar Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Fahad%20N%20Almajhdi"> Fahad N Almajhdi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study provides data on the viral diagnosis and molecular epidemiology of influenza A(H3N2) virus isolated in Riyadh, Saudi Arabia. Nasopharyngeal aspirates from 80 clinically infected patients in the peak of the 2010-2011 winter seasons were processed for viral diagnosis by RT-PCR. Sequencing of entire HA and NA genes of representative isolates and molecular epidemiological analysis were performed. A total of 06 patients were positive for influenza A, B and respiratory syncytial viruses by RT-PCR assays; out of these only one sample was positive for influenza A(H3N2) by RT-PCR. Phylogenetic analysis of the HA and NA gene sequences showed identities higher than 99-98.8 % in both genes. They were also similar to reference isolates in HA sequences (99 % identity) and in NA sequences (99 % identity). Amino acid sequences predicted for the HA gene were highly identical to reference strains. The NA amino acid substitutions identified did not include the oseltamivir-resistant H275Y substitution. Conclusion: Viral isolation and RT-PCR together were useful for diagnosis of the influenza A (H3N2) virus. Variations in HA and NA sequences are similar to those identified in worldwide reference isolates and no drug resistance was found. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=influenza%20A%20%28H3N2%29" title="influenza A (H3N2)">influenza A (H3N2)</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20characterization" title=" genetic characterization"> genetic characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=viral%20isolation" title=" viral isolation"> viral isolation</a>, <a href="https://publications.waset.org/abstracts/search?q=RT-PCR" title=" RT-PCR"> RT-PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=Saudi%20Arabia" title=" Saudi Arabia "> Saudi Arabia </a> </p> <a href="https://publications.waset.org/abstracts/17317/molecular-characterization-and-phylogenetic-analysis-of-influenza-ah3n2-virus-circulating-during-the-2010-2011-in-riyadh-saudi-arabia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17317.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4028</span> Molecular Diagnosis of Influenza Strains Was Carried Out on Patients of the Social Security Clinic in Karaj Using the RT-PCR Technique</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Ferasat">A. Ferasat</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Rostampour%20Yasouri"> S. Rostampour Yasouri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Seasonal flu is a highly contagious infection caused by influenza viruses. These viruses undergo genetic changes that result in new epidemics across the globe. Medical attention is crucial in severe cases, particularly for the elderly, frail, and those with chronic illnesses, as their immune systems are often weaker. The purpose of this study was to detect new subtypes of the influenza A virus rapidly using a specific RT-PCR method based on the HA gene (hemagglutinin). In the winter and spring of 2022_2023, 120 embryonated egg samples were cultured, suspected of seasonal influenza. RNA synthesis, followed by cDNA synthesis, was performed. Finally, the PCR technique was applied using a pair of specific primers designed based on the HA gene. The PCR product was identified after purification, and the nucleotide sequence of purified PCR products was compared with the sequences in the gene bank. The results showed a high similarity between the sequence of the positive samples isolated from the patients and the sequence of the new strains isolated in recent years. This RT-PCR technique is entirely specific in this study, enabling the detection and multiplication of influenza and its subspecies from clinical samples. The RT-PCR technique based on the HA gene, along with sequencing, is a fast, specific, and sensitive diagnostic method for those infected with influenza viruses and its new subtypes. Rapid molecular diagnosis of influenza is essential for suspected people to control and prevent the spread of the disease to others. It also prevents the occurrence of secondary (sometimes fatal) pneumonia that results from influenza and pathogenic bacteria. The critical role of rapid diagnosis of new strains of influenza is to prepare a drug vaccine against the latest viruses that did not exist in the community last year and are entirely new viruses. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=influenza" title="influenza">influenza</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20diagnosis" title=" molecular diagnosis"> molecular diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=patients" title=" patients"> patients</a>, <a href="https://publications.waset.org/abstracts/search?q=RT-PCR%20technique" title=" RT-PCR technique"> RT-PCR technique</a> </p> <a href="https://publications.waset.org/abstracts/177300/molecular-diagnosis-of-influenza-strains-was-carried-out-on-patients-of-the-social-security-clinic-in-karaj-using-the-rt-pcr-technique" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/177300.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">74</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4027</span> Diagnosis of Avian Pathology in the East of Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Khenenou%20Tarek">Khenenou Tarek</a>, <a href="https://publications.waset.org/abstracts/search?q=Benzaoui%20Hassina"> Benzaoui Hassina</a>, <a href="https://publications.waset.org/abstracts/search?q=Melizi%20Mohamed"> Melizi Mohamed </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The diagnosis requires a background of current knowledge in the field and also complementary means in which the laboratory occupies the central place for a better investigation. A correct diagnosis allows to establish the most appropriate treatment as soon as possible and avoids both the economic losses associated with mortality and growth retardation often observed in poultry furthermore it may reduce the high cost of treatment. Epedemiologic survey, hematologic and histopathologic study’s are three aspects of diagnosis heavily used in both human and veterinary pathology and the advanced researches in human medicine would be exploited to be applied in veterinary medicine with given modification .Whereas, the diagnostic methods in the east of Algeria are limited to the clinical signs and necropsy finding. Therefore, the diagnosis is based simply on the success or the failure of the therapeutic methods (therapeutic diagnosis). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chicken" title="chicken">chicken</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=hematology" title=" hematology"> hematology</a>, <a href="https://publications.waset.org/abstracts/search?q=histopathology" title=" histopathology"> histopathology</a> </p> <a href="https://publications.waset.org/abstracts/21640/diagnosis-of-avian-pathology-in-the-east-of-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21640.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">630</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4026</span> Utility of the Loop-Mediated Isothermal Amplification Assay for the Diagnosis of Visceral Leishmaniasis from Blood Samples in Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dawit%20Gebreegzabher%20Hagos">Dawit Gebreegzabher Hagos</a>, <a href="https://publications.waset.org/abstracts/search?q=Yazezew%20Kebede%20Kiro"> Yazezew Kebede Kiro</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmud%20Abdulkader"> Mahmud Abdulkader</a>, <a href="https://publications.waset.org/abstracts/search?q=Henk%20H.%20D.%20F.%20Schallig"> Henk H. D. F. Schallig</a>, <a href="https://publications.waset.org/abstracts/search?q=Dawit%20Wolday"> Dawit Wolday</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapid and accurate visceral leishmaniasis (VL) diagnosis is needed to initiate prompt treatment to reduce morbidity and mortality. Here, we evaluated the performance of loop-mediated isothermal amplification (LAMP) assay for the diagnosis of VL from blood in an endemic area in Ethiopia. LAMP was positive in 117/122 confirmed VL cases and negative in 149/152 controls, resulting in a sensitivity of 95.9% (95% CI: 90.69–98.66) and a specificity of 98.0% (95% CI: 94.34–99.59), respectively. The sensitivity of the LAMP assay was 95.0% (95% CI: 88.61–98.34) in HIV-negatives and 100% (95% CI: 85.18–100.0) in HIV-positives. Compared with microscopy, LAMP detected 82/87 (94.3%, 95% CI: 87.10–98.11) of the microscopy1 cases and was negative in 11/27 (40.7%, 95% CI: 22.39–61.20) of the microscopy2 cases. Compared with the rK39 serology, LAMP detected 113/120 (94.2%, 95% CI: 88.35–97.62) of the rK391 cases and was negative in 149/154 (96.8%, 95% CI: 92.59–98.94) of the rK392 cases. However, when compared with microscopy only, rK39 detected 83/87 (95.4%, 95% CI: 88.64–98.73) of the microscopy1 cases and negative in only 12/27 (44.4%, 95% CI: 25.48–64.67) of the microscopy– cases. There was an excellent agreement between rK39 and LAMP (Kappa 5 0.91, 95% CI: 0.86–0.96). Furthermore, an algorithm using rK39 followed by LAMP would yield a sensitivity of 99.2% (95%CI: 95.52–99.89) and a specificity of 98.0% (95% CI: 94.34–99.59). The findings demonstrate that the LAMP assay is an accurate and rapid molecular assay for VL diagnosis, including in HIV-1 co-infected patients, in an endemic setting. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=visceral%20leishmaniasis" title="visceral leishmaniasis">visceral leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=HIV" title=" HIV"> HIV</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=LAMP" title=" LAMP"> LAMP</a>, <a href="https://publications.waset.org/abstracts/search?q=Ethiopia" title=" Ethiopia"> Ethiopia</a> </p> <a href="https://publications.waset.org/abstracts/162163/utility-of-the-loop-mediated-isothermal-amplification-assay-for-the-diagnosis-of-visceral-leishmaniasis-from-blood-samples-in-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162163.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">98</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4025</span> A Diagnostic Challenge of Drug Resistant Childhood Tuberculosis in Developing World</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Warda%20Fatima">Warda Fatima</a>, <a href="https://publications.waset.org/abstracts/search?q=Hasnain%20Javed"> Hasnain Javed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The emerging trend of Drug resistance in childhood Tuberculosis is increasing worldwide and now becoming a priority challenge for National TB Control Programs of the world. Childhood TB accounts for 10-15% of total TB burden across the globe and same proportion is quantified in case of drug resistant TB. One third population suffering from MDR TB dies annually because of non-diagnosis and unavailability of appropriate treatment. However, true Childhood MDR TB cannot be estimated due to non-confirmation. Diagnosis of Pediatric TB by sputum Smear Microscopy and Culture inoculation are limited due to paucibacillary nature and difficulties in obtaining adequate sputum specimens. Diagnosis becomes more difficult when it comes to HIV infected child. New molecular advancements for early case detection of TB and MDR TB in adults have not been endorsed in children. Multi centered trials are needed to design better diagnostic approaches and efficient and safer treatments for DR TB in high burden countries. The aim of the present study is to sketch out the current situation of the childhood Drug resistant TB especially in the developing world and to highlight the classic and novel methods that are to be implemented in high-burden resource-limited locations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20resistant%20TB" title="drug resistant TB">drug resistant TB</a>, <a href="https://publications.waset.org/abstracts/search?q=childhood" title=" childhood"> childhood</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=novel%20methods" title=" novel methods"> novel methods</a> </p> <a href="https://publications.waset.org/abstracts/18875/a-diagnostic-challenge-of-drug-resistant-childhood-tuberculosis-in-developing-world" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18875.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">401</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4024</span> Rapid Detection and Differentiation of Camel Pox, Contagious Ecthyma and Papilloma Viruses in Clinical Samples of Camels Using a Multiplex PCR</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20I.%20Khalafalla">A. I. Khalafalla</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20A.%20Al-Busada"> K. A. Al-Busada</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20M.%20El-Sabagh"> I. M. El-Sabagh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pox and pox-like diseases of camels are a group of exanthematous skin conditions that have become increasingly important economically. They may be caused by three distinct viruses: camelpox virus (CMPV), camel contagious ecthyma virus (CCEV) and camel papillomavirus (CAPV). These diseases are difficult to differentiate based on clinical presentation in disease outbreaks. Molecular methods such as PCR targeting species-specific genes have been developed and used to identify CMPV and CCEV, but not simultaneously in a single tube. Recently, multiplex PCR has gained reputation as a convenient diagnostic method with cost- and time–saving benefits. In the present communication, we describe the development, optimization and validation a multiplex PCR assays able to detect simultaneously the genome of the three viruses in one single test allowing for rapid and efficient molecular diagnosis. The assay was developed based on the evaluation and combination of published and new primer sets, and was applied to the detection of 110 tissue samples. The method showed high sensitivity, and the specificity was confirmed by PCR-product sequencing. In conclusion, this rapid, sensitive and specific assay is considered a useful method for identifying three important viruses in specimens from camels and as part of a molecular diagnostic regime. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=multiplex%20PCR" title="multiplex PCR">multiplex PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=pox%20and%20pox-like%20diseases" title=" pox and pox-like diseases"> pox and pox-like diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=camels" title=" camels "> camels </a> </p> <a href="https://publications.waset.org/abstracts/23894/rapid-detection-and-differentiation-of-camel-pox-contagious-ecthyma-and-papilloma-viruses-in-clinical-samples-of-camels-using-a-multiplex-pcr" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23894.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">466</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4023</span> Testing Immunochemical Method for the Bacteriological Diagnosis of Bovine Tuberculosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Assiya%20Madenovna%20Borsynbayeva">Assiya Madenovna Borsynbayeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Kairat%20Altynbekovich%20Turgenbayev"> Kairat Altynbekovich Turgenbayev</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolay%20Petrovich%20Ivanov"> Nikolay Petrovich Ivanov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this article presents the results of rapid diagnostics of tuberculosis in comparison with classical bacteriological method. The proposed method of rapid diagnosis of tuberculosis than bacteriological method allows shortening the time of diagnosis to 7 days, to visualize the growth of mycobacteria in the semi-liquid medium and differentiate the type of mycobacterium. Fast definition of Mycobacterium tuberculosis and its derivatives in the culture medium is a new and promising direction in the diagnosis of tuberculosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=animal%20diagnosis%20of%20tuberculosis" title="animal diagnosis of tuberculosis">animal diagnosis of tuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteriological%20diagnostics" title=" bacteriological diagnostics"> bacteriological diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=antigen" title=" antigen"> antigen</a>, <a href="https://publications.waset.org/abstracts/search?q=specific%20antibodies" title=" specific antibodies"> specific antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=immunological%20reaction" title=" immunological reaction"> immunological reaction</a> </p> <a href="https://publications.waset.org/abstracts/46923/testing-immunochemical-method-for-the-bacteriological-diagnosis-of-bovine-tuberculosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46923.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">344</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4022</span> Pathological and Molecular Diagnosis of Caseous Lymphadenitis in Chinkara Deer (Gazella Bennettii), in Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mudassar%20Iqbal">Mudassar Iqbal</a>, <a href="https://publications.waset.org/abstracts/search?q=Riaz%20Hussain"> Riaz Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Khalid%20Mehmood"> Khalid Mehmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Farah%20Ali"> Farah Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Fazal%20Mahmood"> Fazal Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdul%20Ghaffar"> Abdul Ghaffar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Corynebacterium pseudotuberculosis is an important cause of caseous lymphadenitis (CL), a complex, chronic devastating and destructive disease of small ruminants. In present study, postmortem examination of Chinkara deer (n=25) was conducted in year 2014. Pus samples suggestive of CL were collected from the superficial lymph nodes, liver, spleen and lungs during necropsy and subjected to standard microbiological procedures for isolation and molecular analysis of bacterial pathogens. Pus samples collected from carcasses (25) presenting clinical lesions of C. pseudotuberculosis infection was identified in 19 (76%) carcasses on the basis of culture characteristics. The frequency of C. pseudotuberculosis bacterium was higher in older animals as compared to young animals. Grossly, multiple tubercles of variable size having caseous material were observed in liver, lungs, spleen and lymph nodes. Histopathologically, tissue sections from all the visceral organs were extensively plugged with abscess. In present study specific prolineiminopeptidase (PIP) gene of the C. pseudotuberculosis was amplified by the Polymerase chain reaction technique (PCR) in 17(25) cases. The efficient and reliable molecular analysis along with necropsy findings in present study can be used as valuable approach for diagnosis of caseous lymphadenitis in small ruminants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chinkara%20deer" title="Chinkara deer">Chinkara deer</a>, <a href="https://publications.waset.org/abstracts/search?q=Corynebacterium%20pseudotuberculosis" title=" Corynebacterium pseudotuberculosis"> Corynebacterium pseudotuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=Caseous%20lymphadenitis" title=" Caseous lymphadenitis"> Caseous lymphadenitis</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a> </p> <a href="https://publications.waset.org/abstracts/24616/pathological-and-molecular-diagnosis-of-caseous-lymphadenitis-in-chinkara-deer-gazella-bennettii-in-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24616.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">482</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4021</span> Development of Loop Mediated Isothermal Amplification (Lamp) Assay for the Diagnosis of Ovine Theileriosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Fiaz%20Qamar">Muhammad Fiaz Qamar</a>, <a href="https://publications.waset.org/abstracts/search?q=Uzma%20Mehreen"> Uzma Mehreen</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Arfan%20Zaman"> Muhammad Arfan Zaman</a>, <a href="https://publications.waset.org/abstracts/search?q=Kazim%20Ali"> Kazim Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ovine Theileriosis is a world-wide concern, especially in tropical and subtropical areas, due to having tick abundance that has received less awareness in different developed and developing areas due to less worth of sheep, low to the middle level of infection in different small ruminants herd. Across Asia, the prevalence reports have been conducted to provide equivalent calculation of flock and animal level prevalence of Theileriosisin animals. It is a challenge for veterinarians to timely diagnosis & control of Theileriosis and famers because of the nature of the organism and inadequacy of restricted plans to control. All most work is based upon the development of such a technique which should be farmer-friendly, less expensive, and easy to perform into the field. By the timely diagnosis of this disease will decrease the irrational use of the drugs, and other plan was to determine the prevalence of Theileriosis in District Jhang by using the conventional method, PCR and qPCR, and LAMP. We quantify the molecular epidemiology of T.lestoquardiin sheep from Jhang districts, Punjab, Pakistan. In this study, we concluded that the overall prevalence of Theileriosis was (32/350*100= 9.1%) in sheep by using Giemsa staining technique, whereas (48/350*100= 13%) is observed by using PCR technique (56/350*100=16%) in qPCR and the LAMP technique have shown up to this much prevalence percentage (60/350*100= 17.1%). The specificity and sensitivity also calculated in comparison with the PCR and LAMP technique. Means more positive results have been shown when the diagnosis has been done with the help of LAMP. And there is little bit of difference between the positive results of PCR and qPCR, and the least positive animals was by using Giemsa staining technique/conventional method. If we talk about the specificity and sensitivity of the LAMP as compared to PCR, The cross tabulation shows that the results of sensitivity of LAMP counted was 94.4%, and specificity of LAMP counted was 78%. Advances in scientific field must be upon reality based ideas which can lessen the gaps and hurdles in the way of scientific research; the lamp is one of such techniques which have done wonders in adding value and helping human at large. It is such a great biological diagnostic tools and has helped a lot in the proper diagnosis and treatment of certain diseases. Other methods for diagnosis, such as culture techniques and serological techniques, have exposed humans with great danger. However, with the help of molecular diagnostic technique like LAMP, exposure to such pathogens is being avoided in the current era Most prompt and tentative diagnosis can be made using LAMP. Other techniques like PCR has many disadvantages when compared to LAMP as PCR is a relatively expensive, time consuming, and very complicated procedure while LAMP is relatively cheap, easy to perform, less time consuming, and more accurate. LAMP technique has removed hurdles in the way of scientific research and molecular diagnostics, making it approachable to poor and developing countries. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=distribution" title="distribution">distribution</a>, <a href="https://publications.waset.org/abstracts/search?q=thelaria" title=" thelaria"> thelaria</a>, <a href="https://publications.waset.org/abstracts/search?q=LAMP" title=" LAMP"> LAMP</a>, <a href="https://publications.waset.org/abstracts/search?q=primer%20sequences" title=" primer sequences"> primer sequences</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a> </p> <a href="https://publications.waset.org/abstracts/152435/development-of-loop-mediated-isothermal-amplification-lamp-assay-for-the-diagnosis-of-ovine-theileriosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/152435.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">103</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4020</span> The Accuracy of Parkinson's Disease Diagnosis Using [123I]-FP-CIT Brain SPECT Data with Machine Learning Techniques: A Survey</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lavanya%20Madhuri%20Bollipo">Lavanya Madhuri Bollipo</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20V.%20Kadambari"> K. V. Kadambari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: To discuss key issues in the diagnosis of Parkinson disease (PD), To discuss features influencing PD progression, To discuss importance of brain SPECT data in PD diagnosis, and To discuss the essentiality of machine learning techniques in early diagnosis of PD. An accurate and early diagnosis of PD is nowadays a challenge as clinical symptoms in PD arise only when there is more than 60% loss of dopaminergic neurons. So far there are no laboratory tests for the diagnosis of PD, causing a high rate of misdiagnosis especially when the disease is in the early stages. Recent neuroimaging studies with brain SPECT using 123I-Ioflupane (DaTSCAN) as radiotracer shown to be widely used to assist the diagnosis of PD even in its early stages. Machine learning techniques can be used in combination with image analysis procedures to develop computer-aided diagnosis (CAD) systems for PD. This paper addressed recent studies involving diagnosis of PD in its early stages using brain SPECT data with Machine Learning Techniques. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Parkinson%20disease%20%28PD%29" title="Parkinson disease (PD)">Parkinson disease (PD)</a>, <a href="https://publications.waset.org/abstracts/search?q=dopamine%20transporter" title=" dopamine transporter"> dopamine transporter</a>, <a href="https://publications.waset.org/abstracts/search?q=single-photon%20emission%20computed%20tomography%20%28SPECT%29" title=" single-photon emission computed tomography (SPECT)"> single-photon emission computed tomography (SPECT)</a>, <a href="https://publications.waset.org/abstracts/search?q=support%20vector%20machine%20%28SVM%29" title=" support vector machine (SVM)"> support vector machine (SVM)</a> </p> <a href="https://publications.waset.org/abstracts/39159/the-accuracy-of-parkinsons-disease-diagnosis-using-123i-fp-cit-brain-spect-data-with-machine-learning-techniques-a-survey" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39159.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">399</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4019</span> Application of Interval Valued Picture Fuzzy Set in Medical Diagnosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Palash%20Dutta">Palash Dutta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> More frequently uncertainties are encountered in medical diagnosis and therefore it is the most important and interesting area of applications of fuzzy set theory. In this present study, an attempt has been made to extend Sanchez’s approach for medical diagnosis via interval valued picture fuzzy sets and exhibit the technique with suitable case studies. In this article, it is observed that a refusal can be expressed in the databases concerning the examined objects. The technique is performing diagnosis on the basis of distance measures and as a result, this approach makes it possible to introduce weights of all symptoms and consequently patient can be diagnosed directly. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medical%20diagnosis" title="medical diagnosis">medical diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=uncertainty" title=" uncertainty"> uncertainty</a>, <a href="https://publications.waset.org/abstracts/search?q=fuzzy%20set" title=" fuzzy set"> fuzzy set</a>, <a href="https://publications.waset.org/abstracts/search?q=picture%20fuzzy%20set" title=" picture fuzzy set"> picture fuzzy set</a>, <a href="https://publications.waset.org/abstracts/search?q=interval%20valued%20picture%20fuzzy%20set" title=" interval valued picture fuzzy set"> interval valued picture fuzzy set</a> </p> <a href="https://publications.waset.org/abstracts/54935/application-of-interval-valued-picture-fuzzy-set-in-medical-diagnosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54935.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">378</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4018</span> Microarrays: Wide Clinical Utilities and Advances in Healthcare</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Salma%20M.%20Wakil">Salma M. Wakil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Advances in the field of genetics overwhelmed detecting large number of inherited disorders at the molecular level and directed to the development of innovative technologies. These innovations have led to gene sequencing, prenatal mutation detection, pre-implantation genetic diagnosis; population based carrier screening and genome wide analyses using microarrays. Microarrays are widely used in establishing clinical and diagnostic setup for genetic anomalies at a massive level, with the advent of cytoscan molecular karyotyping as a clinical utility card for detecting chromosomal aberrations with high coverage across the entire human genome. Unlike a regular karyotype that relies on the microscopic inspection of chromosomes, molecular karyotyping with cytoscan constructs virtual chromosomes based on the copy number analysis of DNA which improves its resolution by 100-fold. We have been investigating a large number of patients with Developmental Delay and Intellectual disability with this platform for establishing micro syndrome deletions and have detected number of novel CNV’s in the Arabian population with the clinical relevance. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microarrays" title="microarrays">microarrays</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20karyotyping" title=" molecular karyotyping"> molecular karyotyping</a>, <a href="https://publications.waset.org/abstracts/search?q=developmental%20delay" title=" developmental delay"> developmental delay</a>, <a href="https://publications.waset.org/abstracts/search?q=genetics" title=" genetics"> genetics</a> </p> <a href="https://publications.waset.org/abstracts/24137/microarrays-wide-clinical-utilities-and-advances-in-healthcare" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24137.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">456</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4017</span> Phenotypic and Genotypic Diagnosis of Gaucher Disease in Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Hallal">S. Hallal</a>, <a href="https://publications.waset.org/abstracts/search?q=Z.%20Chami"> Z. Chami</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Hadji-Lehtihet"> A. Hadji-Lehtihet</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Sokhal-Boudella"> S. Sokhal-Boudella</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Berhoune"> A. Berhoune</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Yargui"> L. Yargui</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gaucher disease is the most common lysosomal storage in our population, it is due to a deficiency of β –glucosidase acid. The enzyme deficiency causes a pathological accumulation of undegraded substrate in lysosomes. This metabolic overload is responsible for a multisystemic disease with hepatosplenomegaly, anemia, thrombocytopenia, and bone involvement. Neurological involvement is rare. The laboratory diagnosis of Gaucher disease consists of phenotypic diagnosis by determining the enzymatic activity of β - glucosidase by fluorimetric method, a study by genotypic diagnosis in the GBA gene, limiting the search recurrent mutations (N370S, L444P, 84 GG); PCR followed by an enzymatic digestion. Abnormal profiles were verified by sequencing. Monitoring of treated patients is provided by the determination of chitotriosidase. Our experience spaning a period of 6 years (2007-2014) has enabled us to diagnose 78 patients out of a total of 328 requests from the various departments of pediatrics, internal medicine, neurology. Genotypic diagnosis focused on the entire family of 9 children treated at pediatric CHU Mustapha, which help define the clinical form; or 5 of them had type III disease, carrying the L444P mutation in the homozygous state. Three others were composite (N370/L444P) (N370S/other unintended mutation in our study), and only in one family no recurrent mutation has been found. This molecular study permits screening of heterozygous essential for genetic counseling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gaucher%20disease" title="Gaucher disease">Gaucher disease</a>, <a href="https://publications.waset.org/abstracts/search?q=mutations" title=" mutations"> mutations</a>, <a href="https://publications.waset.org/abstracts/search?q=N370S" title=" N370S"> N370S</a>, <a href="https://publications.waset.org/abstracts/search?q=L444P" title=" L444P"> L444P</a> </p> <a href="https://publications.waset.org/abstracts/17819/phenotypic-and-genotypic-diagnosis-of-gaucher-disease-in-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17819.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">405</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4016</span> Profile of Cross-Reactivity Allergens Highlighted by Multiplex Technology “Alex Microchip Technique” in the Diagnosis of Type I Hypersensitivity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gadiri%20Sabiha">Gadiri Sabiha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Current allergy diagnostic tools using Multiplex technology have made it possible to increase the efficiency of the search for specific IgE. This opportunity is provided by the newly developed “Alex Biochip”, consisting of a panel of 282 allergens in native and molecular form, a CCD inhibitor, and the potential for detecting cross-reactive allergens. We evaluated the performance of this technology in detecting cross-reactivity in previously explored patients. Material/Method: The sera of 39 patients presenting sensitization and polysensitization profiles were explored. The search for specific IgE is carried out by the Alex ® IgE Biochip, and the results are analyzed by nature and by molecular family of allergens using specific software. Results/Discussion: The analysis gave a particular profile of cross-reactivity allergens: 33% for the Ole e1 family, 31% for NPC2, 26% for storage proteins, 20% for Tropomyosin, 10% for LTPs, 10% for Arginine Kinase and 10% for Uteroglobin CCDs were absent in all patients. The “Ole e1” allergen is responsible for a pollen-pollen cross allergy. The storage proteins found and LTP are not species-specific, causing cross-pollen-food allergy. The nDer p2 of the NPC2 family is responsible for cross-reactivity between mite species. Conclusion: The cross-reactivities responsible for mixed syndromes at diagnosis in our patients were dominated by pollen-pollen and pollen-food syndromes. They allow the identification of severity factors linked to the prognosis and the best-adapted immunotherapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=specific%20IgE" title="specific IgE">specific IgE</a>, <a href="https://publications.waset.org/abstracts/search?q=allergy" title=" allergy"> allergy</a>, <a href="https://publications.waset.org/abstracts/search?q=cross%20reactivity" title=" cross reactivity"> cross reactivity</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20allergens" title=" molecular allergens"> molecular allergens</a> </p> <a href="https://publications.waset.org/abstracts/172601/profile-of-cross-reactivity-allergens-highlighted-by-multiplex-technology-alex-microchip-technique-in-the-diagnosis-of-type-i-hypersensitivity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/172601.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">66</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4015</span> Thick Disc Molecular Gas Fraction in NGC 6946</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Narendra%20Nath%20Patra">Narendra Nath Patra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Several recent studies reinforce the existence of a thick molecular disc in galaxies along with the dynamically cold thin disc. Assuming a two-component molecular disc, we model the disc of NGC 6946 as a four-component system consists of stars, HI, thin disc molecular gas, and thick disc molecular gas in vertical hydrostatic equilibrium. Following, we set up the joint Poisson-Boltzmann equation of hydrostatic equilibrium and solve it numerically to obtain a three-dimensional density distribution of different baryonic components. Using the density solutions and the observed rotation curve, we further build a three-dimensional dynamical model of the molecular disc and consecutively produce simulated CO spectral cube and spectral width profile. We find that the simulated spectral width profiles distinguishably differs for different assumed thick disc molecular gas fraction. Several CO spectral width profiles are then produced for different assumed thick disc molecular gas fractions and compared with the observed one to obtain the best fit thick disc molecular gas fraction profile. We find that the thick disc molecular gas fraction in NGC 6946 largely remains constant across its molecular disc with a mean value of 0.70 +/- 0.09. We also estimate the amount of extra-planar molecular gas in NGC 6946. We find 60% of the total molecular gas is extra-planar at the central region, whereas this fraction reduces to ~ 35% at the edge of the molecular disc. With our method, for the first time, we estimate the thick disc molecular gas fraction as a function of radius in an external galaxy with sub-kpc resolution. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=galaxies%3A%20kinematics%20and%20dynamic" title="galaxies: kinematics and dynamic">galaxies: kinematics and dynamic</a>, <a href="https://publications.waset.org/abstracts/search?q=galaxies%3A%20spiral" title=" galaxies: spiral"> galaxies: spiral</a>, <a href="https://publications.waset.org/abstracts/search?q=galaxies%3A%20structure" title=" galaxies: structure "> galaxies: structure </a>, <a href="https://publications.waset.org/abstracts/search?q=ISM%3A%20molecules" title=" ISM: molecules"> ISM: molecules</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20data" title=" molecular data"> molecular data</a> </p> <a href="https://publications.waset.org/abstracts/123278/thick-disc-molecular-gas-fraction-in-ngc-6946" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/123278.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">144</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4014</span> Separation and Characterization of Micobacterium bovis Cell Surface Lysate Antigen</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Albina%20V.%20Moskvicheva">Albina V. Moskvicheva</a>, <a href="https://publications.waset.org/abstracts/search?q=Gevorg%20G.%20Kazarian"> Gevorg G. Kazarian</a>, <a href="https://publications.waset.org/abstracts/search?q=Anna%20R.%20Valeeva"> Anna R. Valeeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Marina%20A.%20Efimova"> Marina A. Efimova</a>, <a href="https://publications.waset.org/abstracts/search?q=Malik%20N.%20Mukminov"> Malik N. Mukminov</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduard%20A.%20Shuralev"> Eduard A. Shuralev</a>, <a href="https://publications.waset.org/abstracts/search?q=Rustam%20Kh.%20Ravilov"> Rustam Kh. Ravilov</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamil%20S.%20Khaertynov"> Kamil S. Khaertynov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Improving the early diagnosis of tuberculosis and solving a number of problems associated with the differential diagnosis of Mycobacterium bovis infection, nonspecific tuberculin reactions caused by sensitization of the body by non-tuberculosis mycobacteria, is urgent. The filtrates and extracts of M. bovis cell surface components are promising antigens with diagnostic potential. The purpose of this study was to isolate and characterize antigenic proteins and determine the dominant M. bovis antigens recognized by the humoral immune system. The mycobacterial cells were homogenized on FastPrep-24. Gel-filtration chromatography was used to fractionate the lysates of cell surface component extracts and proteins isolated from M. bovis culture supernatant. The separated fractions were analyzed using two-dimensional gel electrophoresis followed by determination of antigen serological activity using immunoblot with specific hyperimmune rabbit blood serum. As a result of electrophoretic separation of components by molecular weight, 23 antigen fractions were obtained. Analysis of densitograms showed that the fractions contained two zones of antigens with pronounced serological activity, corresponding to molecular weights of 28 and 21 kDa. The high serological activity of the 28 kDa antigen was established by immunoblot using hyperimmune blood sera. Separated and characterized by M. bovis specific antigen with a molecular weight of 28 kDa was added to the collection of specific marker antigens for M. bovis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antigen" title="antigen">antigen</a>, <a href="https://publications.waset.org/abstracts/search?q=gel-filtration%20chromatography" title=" gel-filtration chromatography"> gel-filtration chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=immunoblot" title=" immunoblot"> immunoblot</a>, <a href="https://publications.waset.org/abstracts/search?q=Mycobacterium%20bovis" title=" Mycobacterium bovis"> Mycobacterium bovis</a> </p> <a href="https://publications.waset.org/abstracts/133644/separation-and-characterization-of-micobacterium-bovis-cell-surface-lysate-antigen" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/133644.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4013</span> Molecular Clustering and Velocity Increase in Converging-Diverging Nozzle in Molecular Dynamics Simulation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jeoungsu%20Na">Jeoungsu Na</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaehawn%20Lee"> Jaehawn Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Changil%20Hong"> Changil Hong</a>, <a href="https://publications.waset.org/abstracts/search?q=Suhee%20Kim"> Suhee Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A molecular dynamics simulation in a converging-diverging nozzle was performed to study molecular collisions and their influence to average flow velocity according to a variety of vacuum levels. The static pressures and the dynamic pressure exerted by the molecule collision on the selected walls were compared to figure out the intensity variances of the directional flows. With pressure differences constant between the entrance and the exit of the nozzle, the numerical experiment was performed for molecular velocities and directional flows. The result shows that the velocities increased at the nozzle exit as the vacuum level gets higher in that area because less molecular collisions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cavitation" title="cavitation">cavitation</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20collision" title=" molecular collision"> molecular collision</a>, <a href="https://publications.waset.org/abstracts/search?q=nozzle" title=" nozzle"> nozzle</a>, <a href="https://publications.waset.org/abstracts/search?q=vacuum" title=" vacuum"> vacuum</a>, <a href="https://publications.waset.org/abstracts/search?q=velocity%20increase" title=" velocity increase"> velocity increase</a> </p> <a href="https://publications.waset.org/abstracts/61069/molecular-clustering-and-velocity-increase-in-converging-diverging-nozzle-in-molecular-dynamics-simulation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61069.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">431</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4012</span> Motor Gear Fault Diagnosis by Measurement of Current, Noise and Vibration on AC Machine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sun-Ki%20Hong">Sun-Ki Hong</a>, <a href="https://publications.waset.org/abstracts/search?q=Ki-Seok%20Kim"> Ki-Seok Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Yong-Ho%20Jo"> Yong-Ho Jo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lots of motors have been being used in industry. Therefore many researchers have studied about the failure diagnosis of motors. In this paper, the effect of measuring environment for diagnosis of gear fault connected to a motor shaft is studied. The fault diagnosis is executed through the comparison of normal gear and abnormal gear. The measured FFT data are compared with the normal data and analyzed for q-axis current, noise and vibration. For bad and good environment, the diagnosis results are compared. From these, it is shown that the bad measuring environment may not be able to detect exactly the motor gear fault. Therefore it is emphasized that the measuring environment should be carefully prepared. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=motor%20fault" title="motor fault">motor fault</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=FFT" title=" FFT"> FFT</a>, <a href="https://publications.waset.org/abstracts/search?q=vibration" title=" vibration"> vibration</a>, <a href="https://publications.waset.org/abstracts/search?q=noise" title=" noise"> noise</a>, <a href="https://publications.waset.org/abstracts/search?q=q-axis%20current" title=" q-axis current"> q-axis current</a>, <a href="https://publications.waset.org/abstracts/search?q=measuring%20environment" title=" measuring environment"> measuring environment</a> </p> <a href="https://publications.waset.org/abstracts/32684/motor-gear-fault-diagnosis-by-measurement-of-current-noise-and-vibration-on-ac-machine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32684.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">557</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4011</span> From Biosensors towards Artificial Intelligence: A New Era in Toxoplasmosis Diagnostics and Therapeutics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gehan%20Labib%20Abuelenain">Gehan Labib Abuelenain</a>, <a href="https://publications.waset.org/abstracts/search?q=Azza%20Fahmi"> Azza Fahmi</a>, <a href="https://publications.waset.org/abstracts/search?q=Salma%20Awad%20Mahmoud"> Salma Awad Mahmoud</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Toxoplasmosis is a global parasitic disease caused by the protozoan Toxoplasma gondii (T. gondii), with a high infection rate that affects one third of the human population and results in severe implications in pregnant women, neonates, and immunocompromised patients. Anti-parasitic treatments and schemes available against toxoplasmosis have barely evolved over the last two decades. The available T. gondii therapeutics cannot completely eradicate tissue cysts produced by the parasite and are not well-tolerated by immunocompromised patients. This work aims to highlight new trends in Toxoplasma gondii diagnosis by providing a comprehensive overview of the field, summarizing recent findings, and discussing the new technological advancements in toxoplasma diagnosis and treatment. Advancements in therapeutics utilizing trends in molecular biophysics, such as biosensors, epigenetics, and artificial intelligence (AI), might provide solutions for disease management and prevention. These insights will provide tools to identify research gaps and proffer planning options for disease control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=toxoplamosis" title="toxoplamosis">toxoplamosis</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=therapeutics" title=" therapeutics"> therapeutics</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensors" title=" biosensors"> biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=AI" title=" AI"> AI</a> </p> <a href="https://publications.waset.org/abstracts/186462/from-biosensors-towards-artificial-intelligence-a-new-era-in-toxoplasmosis-diagnostics-and-therapeutics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186462.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">35</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4010</span> Application of Sub-health Diagnosis and Reasoning Method for Avionics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Weiran%20An">Weiran An</a>, <a href="https://publications.waset.org/abstracts/search?q=Junyou%20Shi"> Junyou Shi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Health management has become one of the design goals in the research and development of new generation avionics systems, and is an important complement and development for the testability and fault diagnosis technology. Currently, the research and application for avionics system health dividing and diagnosis technology is still at the starting stage, lack of related technologies and methods reserve. In this paper, based on the health three-state dividing of avionics products, state lateral transfer coupling modeling and diagnosis reasoning method considering sub-health are researched. With the study of typical case application, the feasibility and correctness of the method and the software are verified. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sub-health" title="sub-health">sub-health</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis%20reasoning" title=" diagnosis reasoning"> diagnosis reasoning</a>, <a href="https://publications.waset.org/abstracts/search?q=three-valued%20coupled%20logic" title=" three-valued coupled logic"> three-valued coupled logic</a>, <a href="https://publications.waset.org/abstracts/search?q=extended%20dependency%20model" title=" extended dependency model"> extended dependency model</a>, <a href="https://publications.waset.org/abstracts/search?q=avionics" title=" avionics"> avionics</a> </p> <a href="https://publications.waset.org/abstracts/27329/application-of-sub-health-diagnosis-and-reasoning-method-for-avionics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27329.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">333</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4009</span> Molecular Characterization of Cysticercus tenuicolis of Slaughtered Livestock in Upper-Egypt Governorates</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mosaab%20A.%20Omara">Mosaab A. Omara</a>, <a href="https://publications.waset.org/abstracts/search?q=Layla%20O.%20Elmajdoubb"> Layla O. Elmajdoubb</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Saleh%20Al-Aboodyc"> Mohammad Saleh Al-Aboodyc</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20ElSifyd"> Ahmed ElSifyd</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20O.%20Elkhtamd"> Ahmed O. Elkhtamd</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study is to present the molecular characterization of cysticercus tenuicolis of Taenia hydatigena from livestock isolates in Egypt, using the amplification of sequencing of the mt-CO1 gene. We introduce a detailed image of the Cysticercus tenuicolis infection in ruminant animals in Upper Egypt. Cysticercus tenuicolis inhabits such organs in ruminants as the omentum, viscera, and liver. In the present study, the infection rate of Cysticercus tenuicolis was found to be 16% and 19% in sheep and goat sample respectively. Firstly we report one larval stage of Taenia hydatigena detected in the camel liver in Egypt. Cysticercus tenuicolis infection manifested a higher prevalence in females than in males. Those above 2 years of age manifested a higher infection rate than younger animals. The preferred site for the infection was the omentum: a 70% preference in sheep and a 68% preference in goat samples. The molecular characterization using the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene of isolates from sheep, goats and camels corresponded to T. hydatigena. For this study, molecular characterizations of T. hydatigena were done for the first time in Egypt. Molecular tools are of great assistance in characterizing the Cysticercus tenuicolis parasite especially when the morphological character cannot be detected because the metacestodes are frequently confused with infection by the Hydatid cyst, especially when these occur in the visceral organs. In the present study, Cysticercus tenuicolis manifested high identity in the goat and sheep samples, while differences were found more frequently in the camel samples (10 pairbase). Clearly molecular diagnosis for Cysticercus tenuicolis infection significantly helps to differentiate it from such other metacestodes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cysticercus%20tenuicolis" title="cysticercus tenuicolis">cysticercus tenuicolis</a>, <a href="https://publications.waset.org/abstracts/search?q=its2" title=" its2"> its2</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic" title=" genetic"> genetic</a>, <a href="https://publications.waset.org/abstracts/search?q=qena" title=" qena"> qena</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20and%20taenia%20hydatigena" title=" molecular and taenia hydatigena"> molecular and taenia hydatigena</a> </p> <a href="https://publications.waset.org/abstracts/30593/molecular-characterization-of-cysticercus-tenuicolis-of-slaughtered-livestock-in-upper-egypt-governorates" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30593.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">523</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4008</span> Role of DatScan in the Diagnosis of Parkinson's Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shraddha%20Gopal">Shraddha Gopal</a>, <a href="https://publications.waset.org/abstracts/search?q=Jayam%20Lazarus"> Jayam Lazarus</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aims: To study the referral practice and impact of DAT-scan in the diagnosis or exclusion of Parkinson’s disease. Settings and Designs: A retrospective study Materials and methods: A retrospective study of the results of 60 patients who were referred for a DAT scan over a period of 2 years from the Department of Neurology at Northern Lincolnshire and Goole NHS trust. The reason for DAT scan referral was noted under 5 categories against Parkinson’s disease; drug-induced Parkinson’s, essential tremors, diagnostic dilemma, not responding to Parkinson’s treatment, and others. We assessed the number of patients who were diagnosed with Parkinson’s disease against the number of patients in whom Parkinson’s disease was excluded or an alternative diagnosis was made. Statistical methods: Microsoft Excel was used for data collection and statistical analysis, Results: 30 of the 60 scans were performed to confirm the diagnosis of early Parkinson’s disease, 13 were done to differentiate essential tremors from Parkinsonism, 6 were performed to exclude drug-induced Parkinsonism, 5 were done to look for alternative diagnosis as the patients were not responding to anti-Parkinson medication and 6 indications were outside the recommended guidelines. 55% of cases were confirmed with a diagnosis of Parkinson’s disease. 43.33% had Parkinson’s disease excluded. 33 of the 60 scans showed bilateral abnormalities and confirmed the clinical diagnosis of Parkinson’s disease. Conclusion: DAT scan provides valuable information in confirming Parkinson’s disease in 55% of patients along with excluding the diagnosis in 43.33% of patients aiding an alternative diagnosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DATSCAN" title="DATSCAN">DATSCAN</a>, <a href="https://publications.waset.org/abstracts/search?q=Parkinson%27s%20disease" title=" Parkinson's disease"> Parkinson's disease</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=essential%20tremors" title=" essential tremors"> essential tremors</a> </p> <a href="https://publications.waset.org/abstracts/139742/role-of-datscan-in-the-diagnosis-of-parkinsons-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139742.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">232</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4007</span> A Framework for Early Differential Diagnosis of Tropical Confusable Diseases Using the Fuzzy Cognitive Map Engine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Faith-Michael%20E.%20Uzoka">Faith-Michael E. Uzoka</a>, <a href="https://publications.waset.org/abstracts/search?q=Boluwaji%20A.%20Akinnuwesi"> Boluwaji A. Akinnuwesi</a>, <a href="https://publications.waset.org/abstracts/search?q=Taiwo%20Amoo"> Taiwo Amoo</a>, <a href="https://publications.waset.org/abstracts/search?q=Flora%20Aladi"> Flora Aladi</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephen%20Fashoto"> Stephen Fashoto</a>, <a href="https://publications.waset.org/abstracts/search?q=Moses%20Olaniyan"> Moses Olaniyan</a>, <a href="https://publications.waset.org/abstracts/search?q=Joseph%20Osuji"> Joseph Osuji</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The overarching aim of this study is to develop a soft-computing system for the differential diagnosis of tropical diseases. These conditions are of concern to health bodies, physicians, and the community at large because of their mortality rates, and difficulties in early diagnosis due to the fact that they present with symptoms that overlap, and thus become ‘confusable’. We report on the first phase of our study, which focuses on the development of a fuzzy cognitive map model for early differential diagnosis of tropical diseases. We used malaria as a case disease to show the effectiveness of the FCM technology as an aid to the medical practitioner in the diagnosis of tropical diseases. Our model takes cognizance of manifested symptoms and other non-clinical factors that could contribute to symptoms manifestations. Our model showed 85% accuracy in diagnosis, as against the physicians’ initial hypothesis, which stood at 55% accuracy. It is expected that the next stage of our study will provide a multi-disease, multi-symptom model that also improves efficiency by utilizing a decision support filter that works on an algorithm, which mimics the physician’s diagnosis process. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medical%20diagnosis" title="medical diagnosis">medical diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=tropical%20diseases" title=" tropical diseases"> tropical diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=fuzzy%20cognitive%20map" title=" fuzzy cognitive map"> fuzzy cognitive map</a>, <a href="https://publications.waset.org/abstracts/search?q=decision%20support%20filters" title=" decision support filters"> decision support filters</a>, <a href="https://publications.waset.org/abstracts/search?q=malaria%20differential%20diagnosis" title=" malaria differential diagnosis"> malaria differential diagnosis</a> </p> <a href="https://publications.waset.org/abstracts/44807/a-framework-for-early-differential-diagnosis-of-tropical-confusable-diseases-using-the-fuzzy-cognitive-map-engine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44807.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">319</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4006</span> Molecular Junctions between Graphene Strips: Electronic and Transport Properties</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adel%20Belayadi">Adel Belayadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Mougari"> Ahmed Mougari</a>, <a href="https://publications.waset.org/abstracts/search?q=Boualem%20Bourahla"> Boualem Bourahla</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Molecular junctions are currently considered a promising style in the miniaturization of electronic devices. In this contribution, we provide a tight-binding model to investigate the quantum transport properties across-molecular junctions sandwiched between 2D-graphene nanoribbons in the zigzag direction. We investigate, in particular, the effect of embedded atoms such as Gold and Silicon across the molecular junction. The results exhibit a resonance behavior in terms of incident Fermi levels, depending on the molecular junction type. Additionally, the transport properties under a perpendicular magnetic field exhibit an oscillation for the transmittance versus the magnetic field strength. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=molecular%20junction" title="molecular junction">molecular junction</a>, <a href="https://publications.waset.org/abstracts/search?q=2D-graphene%20nanoribbons" title=" 2D-graphene nanoribbons"> 2D-graphene nanoribbons</a>, <a href="https://publications.waset.org/abstracts/search?q=quantum%20transport%20properties" title=" quantum transport properties"> quantum transport properties</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20field" title=" magnetic field"> magnetic field</a> </p> <a href="https://publications.waset.org/abstracts/157729/molecular-junctions-between-graphene-strips-electronic-and-transport-properties" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157729.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">96</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4005</span> Intelligent System for Diagnosis Heart Attack Using Neural Network</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Oluwaponmile%20David%20Alao">Oluwaponmile David Alao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Misdiagnosis has been the major problem in health sector. Heart attack has been one of diseases that have high level of misdiagnosis recorded on the part of physicians. In this paper, an intelligent system has been developed for diagnosis of heart attack in the health sector. Dataset of heart attack obtained from UCI repository has been used. This dataset is made up of thirteen attributes which are very vital in diagnosis of heart disease. The system is developed on the multilayer perceptron trained with back propagation neural network then simulated with feed forward neural network and a recognition rate of 87% was obtained which is a good result for diagnosis of heart attack in medical field. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=heart%20attack" title="heart attack">heart attack</a>, <a href="https://publications.waset.org/abstracts/search?q=artificial%20neural%20network" title=" artificial neural network"> artificial neural network</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=intelligent%20system" title=" intelligent system"> intelligent system</a> </p> <a href="https://publications.waset.org/abstracts/33844/intelligent-system-for-diagnosis-heart-attack-using-neural-network" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/33844.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">655</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4004</span> Application of Neural Petri Net to Electric Control System Fault Diagnosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sadiq%20J.%20Abou-Loukh">Sadiq J. Abou-Loukh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present work deals with implementation of Petri nets, which own the perfect ability of modeling, are used to establish a fault diagnosis model. Fault diagnosis of a control system received considerable attention in the last decades. The formalism of representing neural networks based on Petri nets has been presented. Neural Petri Net (NPN) reasoning model is investigated and developed for the fault diagnosis process of electric control system. The proposed NPN has the characteristics of easy establishment and high efficiency, and fault status within the system can be described clearly when compared with traditional testing methods. The proposed system is tested and the simulation results are given. The implementation explains the advantages of using NPN method and can be used as a guide for different online applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=petri%20net" title="petri net">petri net</a>, <a href="https://publications.waset.org/abstracts/search?q=neural%20petri%20net" title=" neural petri net"> neural petri net</a>, <a href="https://publications.waset.org/abstracts/search?q=electric%20control%20system" title=" electric control system"> electric control system</a>, <a href="https://publications.waset.org/abstracts/search?q=fault%20diagnosis" title=" fault diagnosis"> fault diagnosis</a> </p> <a href="https://publications.waset.org/abstracts/16653/application-of-neural-petri-net-to-electric-control-system-fault-diagnosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16653.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">474</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4003</span> The Fast Diagnosis of Acanthamoeba Keratitis Using Real-Time PCR Assay</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fadime%20Eroglu">Fadime Eroglu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acanthamoeba genus belongs to kingdom protozoa, and it is known as free-living amoebae. Acanthamoeba genus has been isolated from human bodies, swimming pools, bottled mineral water, contact lens solutions, dust, and soil. The members of the genus Acanthamoeba causes Acanthamoeba Keratitis which is a painful sight-threatening disease of the eyes. In recent years, the prevalence of Acanthamoeba keratitis has been high rate reported. The eight different Acanthamoeba species are known to be effective in Acanthamoeba keratitis. These species are Acanthamoeba castellanii, Acanthamoeba polyphaga, Acanthamoeba griffini, Acanthamoeba hatchetti, Acanthamoeba culbertsoni and Acanhtamoeba rhysodes. The conventional diagnosis of Acanthamoeba Keratitis has relied on cytological preparations and growth of Acanthamoeba in culture. However molecular methods such as real-time PCR has been found to be more sensitive. The real-time PCR has now emerged as an effective method for more rapid testing for the diagnosis of infectious disease in decade. Therefore, a real-time PCR assay for the detection of Acanthamoeba keratitis and Acanthamoeba species have been developed in this study. The 18S rRNA sequences from Acanthamoeba species were obtained from National Center for Biotechnology Information and sequences were aligned with MEGA 6 programme. Primers and probe were designed using Custom Primers-OligoPerfectTMDesigner (ThermoFisherScientific, Waltham, MA, USA). They were also assayed for hairpin formation and degree of primer-dimer formation with Multiple Primer Analyzer ( ThermoFisherScientific, Watham, MA, USA). The eight different ATCC Acanthamoeba species were obtained, and DNA was extracted using the Qiagen Mini DNA extraction kit (Qiagen, Hilden, Germany). The DNA of Acanthamoeba species were analyzed using newly designed primer and probe set in real-time PCR assay. The early definitive laboratory diagnosis of Acanthamoeba Keratitis and the rapid initiation of suitable therapy is necessary for clinical prognosis. The results of the study have been showed that new primer and probes could be used for detection and distinguish for Acanthamoeba species. These new developing methods are helpful for diagnosis of Acanthamoeba Keratitis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acathamoeba%20Keratitis" title="Acathamoeba Keratitis">Acathamoeba Keratitis</a>, <a href="https://publications.waset.org/abstracts/search?q=Acanthamoeba%20species" title=" Acanthamoeba species"> Acanthamoeba species</a>, <a href="https://publications.waset.org/abstracts/search?q=fast%20diagnosis" title=" fast diagnosis"> fast diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=Real-Time%20PCR" title=" Real-Time PCR"> Real-Time PCR</a> </p> <a href="https://publications.waset.org/abstracts/85716/the-fast-diagnosis-of-acanthamoeba-keratitis-using-real-time-pcr-assay" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/85716.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">119</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4002</span> Molecular Characterization of Dirofilaria repens in Dogs from Karnataka, India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20S.%20Malatesh">D. S. Malatesh</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20J.%20Ananda"> K. J. Ananda</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Ansar%20Kamran"> C. Ansar Kamran</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Ganesh%20Udupa"> K. Ganesh Udupa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dirofilaria repens is a mosquito-borne filarioid nematode of dogs and other carnivores and accidentally affects humans. D. repens is reported in many countries, including India. Subcutaneous dirofilariosis caused by D. repens is a zoonotic disease, widely distributed throughout Europe, Asia, and Africa, with higher prevalence reported in dogs from Sri Lanka (30-60%), Iran (61%) and Italy (21-25%). Dirofilariasis in dogs was diagnosed by detection of microfilariae in blood. Identification of different Dirofilaria species was done by using molecular methods like polymerase chain reaction (PCR). Even though many researchers reported molecular evidence of D. repens across India, to our best knowledge there is no data available on molecular diagnosis of D. repens in dogs and its zoonotic implication in Karnataka state a southern state in India. The aim of the present study was to identify the Dirofilaria species occurring in dogs from Karnataka, India. Out of 310 samples screened for the presence of microfilariae using traditional diagnostic methods, 99 (31.93%) were positive for the presence of microfilariae. Based on the morphometry, the microfilariae were identified as D. repens. For confirmation of species, the samples were subjected to PCR using pan filarial primers (DIDR-F1, DIDR-R1) for amplification of internal transcribed spacer region 2 (ITS2) of the ribosomal DNA. The PCR product of 484 base pairs on agarose gel was indicative of D. repens. Hence, a single PCR reaction using pan filarial primers can be used to differentiate filarial species found in dogs. The present study confirms that dirofilarial species occurring in dogs from Karnataka is D. repens and further sequencing studies are needed for genotypic characterization of D. repens. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dirofilaria%20repens" title="Dirofilaria repens">Dirofilaria repens</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20characterization" title=" molecular characterization"> molecular characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=polymerase%20chain%20reaction" title=" polymerase chain reaction"> polymerase chain reaction</a>, <a href="https://publications.waset.org/abstracts/search?q=Karnataka" title=" Karnataka"> Karnataka</a>, <a href="https://publications.waset.org/abstracts/search?q=India" title=" India"> India</a> </p> <a href="https://publications.waset.org/abstracts/109804/molecular-characterization-of-dirofilaria-repens-in-dogs-from-karnataka-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/109804.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">142</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4001</span> Clinical and Molecular Characterization of Mycoplasmosis in Sheep in Egypt</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Walid%20Mousa">Walid Mousa</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Nayel"> Mohamed Nayel</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Zaghawa"> Ahmed Zaghawa</a>, <a href="https://publications.waset.org/abstracts/search?q=Akram%20Salama"> Akram Salama</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20El-Sify"> Ahmed El-Sify</a>, <a href="https://publications.waset.org/abstracts/search?q=Hesham%20Rashad"> Hesham Rashad</a>, <a href="https://publications.waset.org/abstracts/search?q=Dina%20El-Shafey"> Dina El-Shafey</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mycoplasmosis in small ruminants constitutes a serious contagious problem in smallholders causing severe economic losses worldwide. This study was conducted to determine the clinical, Minimum Inhibitory Concentration (MIC) and molecular characterization of Mycoplasma species associated in sheep breeding herds in Menoufiya governorate, Egypt. Out of the examination of 400 sheep, 104 (26%) showed respiratory manifestations, nasal discharges, cough and conjunctivitis with systemic body reaction. Meanwhile, out of these examined sheep, only 56 (14%) were positive for mycoplasma isolation onto PPLO(Pleuropneumonia-like organisms) specific medium. The MIC for evaluating the efficacy of sensitivity of Mycoplasma isolates against different antibiotics groups revealed that both the Linospectin and Tylosin with 2ug, 0.25ug/ml concentration were the most effective antibiotics for Mycoplasma isolates. The application of PCR was the rapid, specific and sensitive molecular approach for detection of M. ovipneumoniae, and M. arginine at 390 and 326 bp, respectively, in all tested isolates. In conclusion, the diagnosis of Mycoplsamosis in sheep is important to achieve effective control measures and minimizing the disease dissemination among sheep herds. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=MIC" title="MIC">MIC</a>, <a href="https://publications.waset.org/abstracts/search?q=mycoplasmosis" title=" mycoplasmosis"> mycoplasmosis</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=sheep" title=" sheep"> sheep</a> </p> <a href="https://publications.waset.org/abstracts/136307/clinical-and-molecular-characterization-of-mycoplasmosis-in-sheep-in-egypt" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/136307.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge 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