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et_pb_button_alignment_center et_pb_button_alignment_tablet_left et_pb_button_alignment_phone_left et_pb_module "> <a class="et_pb_button et_pb_button_0 et_pb_bg_layout_light" href="https://biopharmawebinars.com/webinars/adventitious-agent-testing-for-mmv-how-much-do-we-really-know/#webinar_embed" target="_blank" data-icon="5" rel="noopener">CLICK HERE TO BOOK YOUR FREE SEAT</a> </div><div class="et_pb_module et_pb_text et_pb_text_0 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h1 style="text-align: center;"><strong><span style="color: #000080;">JOINT WEBINAR</span></strong></h1> <p><b><span style="color: #000080;"></span></b></p> <h2 style="text-align: center;"><strong><span style="color: #000080;">Takeda, PathoQuest & Charles River Laboratories</span></strong></h2> <h1 style="text-align: center;"><b> </b></h1> <h1 style="text-align: center;"><span style="color: #ff9900;"><strong>Adventitious Agent Testing For MMV:</strong></span></h1> <h1 style="text-align: center;"><span style="color: #ff9900;"><strong>How Much Do We Really Know?</strong></span></h1> <p> </p> <p style="text-align: center;"><strong>01st October 2024</strong></p> <p style="text-align: center;">10:00am EDT / 7:00am PDT / 3:00pm BST / 4:00pm CEST</p> <p style="text-align: center;"><span style="color: #808080;"><em>Presented by:</em></span></p> <p style="text-align: center;"><span style="color: #808080;"><em><b>Maria Farcet</b>, Head of Cell Culture, Virus Models & Serology in the Global Pathogen Safety /</em></span></p> <p style="text-align: center;"><span style="color: #808080;"><em>Global Quality Team at <span style="color: #00ccff;"><strong>Takeda</strong></span></em></span></p> <p style="text-align: center;"><span style="color: #808080;"><em>featuring</em></span></p> <p style="text-align: center;"><span style="color: #808080;"><em><strong>Amina Cherif Louazani, </strong>Ph.D., Viral Safety Manager at <span style="color: #00ccff;"><strong>PathoQuest</strong></span></em></span></p> <p style="text-align: center;"><span style="color: #808080;"><em>and </em></span></p> <p style="text-align: center;"><span style="color: #808080;"><em><strong>Dr. Denise Teber</strong>, Scientific Expert of the PCR department at <span style="color: #00ccff;"><strong>Charles River Laboratories</strong></span></em></span></p> <p style="text-align: center;"></div> </div><div class="et_pb_module et_pb_text et_pb_text_1 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: justify;"><strong>Minute virus of mice (MMV)</strong> has contaminated biotechnological processes in the past, making <strong>specific MMV testing</strong> recommended if the production cell line is known to be permissive for this virus. Testing is widely done using <strong>cell-culture based adventitious virus assays</strong>, yet MMV strains may differ in their in vitro cell tropism. Here, I present <strong>growth characteristics of different MMV strains</strong> on A9 and 324K cells and identified significant differences in susceptibility of these widely used indicator cell lines to infection by different strains of MMV, which has implications for MMV detectability during routine testing of biotechnology process harvests[1].</p> <p style="text-align: justify;">An <strong>MMV specific PCR</strong> was evaluated as a more encompassing method and was shown as a suitable replacement for cell culture-based detection of the different MMV strains. This method not only provides <strong>more rapid detection</strong> but also covers other rodent parvoviruses that might contaminate biotechnological processes. Although no MMV contamination event of human-derived cell lines has been reported in the past, biotechnological processes that use these lines, such as <strong>HEK293</strong>, commonly used in biopharmaceutical manufacturing, also need to consider MMV-specific testing as we showed them to be <strong>susceptible to productive MMV infection.</strong></p> <p style="text-align: justify;">[1] Farcet et al., Biotechnology & Bioengineering 2024; 121:131-138; DOI: 10.1002/bit.28573</p> <p style="text-align: center;"></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_0 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_2"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_2 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_button_module_wrapper et_pb_button_1_wrapper et_pb_button_alignment_center et_pb_button_alignment_tablet_left et_pb_button_alignment_phone_left et_pb_module "> <a class="et_pb_button et_pb_button_1 et_pb_bg_layout_light" href="">SPEAKERS</a> </div> </div> </div><div class="et_pb_row et_pb_row_3"> <div class="et_pb_column et_pb_column_1_3 et_pb_column_3 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_image et_pb_image_1"> <span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="293" height="293" src="https://pathoquest.com/wp-content/uploads/2024/08/Pic-MAria.jpeg" alt="" title="Pic MAria" srcset="https://pathoquest.com/wp-content/uploads/2024/08/Pic-MAria.jpeg 293w, https://pathoquest.com/wp-content/uploads/2024/08/Pic-MAria-150x150.jpeg 150w" sizes="(max-width: 293px) 100vw, 293px" class="wp-image-1830" /></span> </div><div class="et_pb_module et_pb_team_member et_pb_team_member_0 clearfix et_pb_bg_layout_light et_pb_team_member_no_image"> <div class="et_pb_team_member_description"> <h4 class="et_pb_module_header">Maria Farcet</h4> <p class="et_pb_member_position">Head of Cell Culture, Virus Models & Serology in the Global Pathogen Safety / Global Quality team at Takeda</p> <div><p style="text-align: justify;">Maria Farcet is Head of Cell Culture, Virus Models & Serology in the Global Pathogen Safety / Global Quality team of Takeda in Vienna, Austria. In her 15+ year career in this field she gained profound expertise in virology and virus safety margins of biological medicinal products. She currently oversees the establishment and operation of virus test systems to enable virus reduction studies, virus testing and virus neutralization assays, under GMP/GLP.</p> <p style="text-align: center;"></div> </div> </div> </div><div class="et_pb_column et_pb_column_1_3 et_pb_column_4 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_image et_pb_image_2"> <span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="293" height="300" src="https://pathoquest.com/wp-content/uploads/2024/08/Pic-Amina.jpg" alt="" title="Pic Amina" class="wp-image-1831" /></span> </div><div class="et_pb_module et_pb_team_member et_pb_team_member_1 clearfix et_pb_bg_layout_light et_pb_team_member_no_image"> <div class="et_pb_team_member_description"> <h4 class="et_pb_module_header">Amina Cherif Louazani, PhD</h4> <p class="et_pb_member_position">Viral Safety Manager at PathoQuest</p> <div><p style="text-align: justify;">Amina Cherif Louazani (PharmD, PhD), Viral Safety Manager at PathoQuest, has a strong background in virology and bioinformatics. She holds a PhD in Infectious Diseases from Aix-Marseille University, France, and specializes in NGS data analysis and protocol development for viral transcriptomes. Amina has published several peer-reviewed articles and held various research and clinical roles. At PathoQuest, she leverages her expertise to provide expert reviews of viral safety assessments based on the iDTECT® platform of NGS assays.</p> <p style="text-align: center;"></div> </div> </div> </div><div class="et_pb_column et_pb_column_1_3 et_pb_column_5 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_image et_pb_image_3"> <span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="293" height="293" src="https://pathoquest.com/wp-content/uploads/2024/08/Pic-Denise.jpg" alt="" title="Pic Denise" srcset="https://pathoquest.com/wp-content/uploads/2024/08/Pic-Denise.jpg 293w, https://pathoquest.com/wp-content/uploads/2024/08/Pic-Denise-150x150.jpg 150w" sizes="(max-width: 293px) 100vw, 293px" class="wp-image-1832" /></span> </div><div class="et_pb_module et_pb_team_member et_pb_team_member_2 clearfix et_pb_bg_layout_light et_pb_team_member_no_image"> <div class="et_pb_team_member_description"> <h4 class="et_pb_module_header">Dr. Denise Teber</h4> <p class="et_pb_member_position">Scientific Expert of the PCR department at Charles River Laboratories</p> <div>Dr. Denise Teber is the Scientific Expert of the PCR department at Charles River, Erkrath, Germany. She is responsible for the establishment of new qPCR and ddPCR assays for the detection of pathogens or process-related biological impurities, as well as method validation of platform assays or client-specific requests. She has a biology degree from the University of Münster and a Master’s degree from the University of Cologne. During an external master thesis at the German Aerospace Center (DLR), she investigated the influence of ionizing radiation on cell cycle progression to predict its harmful impact on astronauts on the International Space Station – ISS. During her PhD and post-doctoral work at the Max Planck Institute for Molecular Biomedicine in Münster, she used lentiviral and adenoviral vectors to induce putative mutations causing venous malformations in isolated primary mouse endothelial cells and analyzed their effects by qPCR, co-immunoprecipitation and western blot as well as intravital microscopy.</p> <p style="text-align: center;"></div> </div> </div> </div> </div><div class="et_pb_row et_pb_row_4"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_6 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_button_module_wrapper et_pb_button_2_wrapper et_pb_button_alignment_center et_pb_button_alignment_tablet_left et_pb_button_alignment_phone_left et_pb_module "> <a class="et_pb_button et_pb_button_2 et_pb_bg_layout_light" href="https://biopharmawebinars.com/webinars/adventitious-agent-testing-for-mmv-how-much-do-we-really-know/#webinar_embed" target="_blank" data-icon="5" rel="noopener">CLICK HERE TO BOOK 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