MultiOmyx™ | NeoGenomics Laboratories

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block block-views block-views-blockgroup-header-block-1" id="block-views-block-group-header-block-1"> <div><div class="view view-group-header view-id-group_header view-display-id-block_1 js-view-dom-id-b23eb68affb67e7bb4d71da051bc7f909f5cd8f0620d0df70882429dd8a0157f"> <div class="view-content"> <div class="views-row"><div class="views-field views-field-field-media-image-3-1"><div class="field-content"><div class="node-header large"> <div class="node-header-image" data-node-header-image="/sites/default/files/hero-image/MO-landing-page.jpg"> <div class="node-header-tab"></div> <div class="node-header-content"> <div class="node-header-wrap"> <h1 class="large">MultiOmyx™</h1> <h4 class="page-subtitle">Proprietary, high-order multiplexing methodology</h4> </div> </div> </div> </div></div></div></div> </div> </div> </div> </div> <div class="views-element-container block block-views block-views-blockdefault-header-block-1" id="block-views-block-default-header-block-1"> <div><div class="view view-default-header view-id-default_header view-display-id-block_1 js-view-dom-id-751854fedba321358e43874b2ca9837cdd5294b2e9f0eeaf794de511e34ae532"> </div> </div> </div> </div> </div> </div> </div> <div id="layout-above-content"> <div class="wrap-above-content"> <div class="above-content-content"> <div class="region region-above-content"> <div id="block-neogenomics-breadcrumbs" class="block block-system block-system-breadcrumb-block"> <nav class="breadcrumb" role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/pharma-services">Pharma Services</a> </li> <li> <a href="/pharma-services/lab-services">Lab Services</a> </li> <li> MultiOmyx™</li> </ol> </nav> </div> </div> </div> </div> </div> <main role="main" class="layout-main"> <div class="wrap-main"> <div class="main-content"> <section class="no-sidebar content-wrap"> <div class="layout-content"> <a id="main-content" tabindex="-1"></a> <div class="region region-content"> <div data-drupal-messages-fallback class="hidden"></div> <div id="block-neogenomics-content" class="block block-system block-system-main-block"> <article data-history-node-id="1147" class="node node--type-promo node--view-mode-full"> <div class="node__content"> <div class="field field--name-field-section field--type-entity-reference-revisions field--label-hidden field__items"> <div class="paragraph paragraph--type--columns paragraph--view-mode--default corporate-pattern-bg"> <div id="" class="col-half padding-top-bottom-100"> <div class="field field--name-field-column-content field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <div class="paragraph paragraph--type--wysiwyg paragraph--view-mode--default"> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><p class="large">MultiOmyx<sup>™</sup>: proprietary, high-order multiplexing methodology</p> </div> </div> </div> <div class="field__item"> <div class="paragraph paragraph--type--wysiwyg paragraph--view-mode--default"> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><p>MultiOmyx<sup>™</sup> enables visualization and characterization of up to 60 proteins in a single formalin fixed, paraffin embedded (FFPE) 4μm tissue section. Slides are prepared and stained using MultiOmyx<sup>™</sup> multiplexing IF staining protocol. For each round of staining, conjugated fluorescent antibodies are applied to the slide, followed by image acquisition of stained slides. The dye is erased, enabling a subsequent round of staining with another pair of fluorescent antibodies.</p> <p> </p> <p><strong>Download the MultiOmyx Panel Brochure:</strong><br> Using our broad array of verified MultiOmyx panels (TIL, TLS, etc.) in your study</p> <p><a class="btn trigger-webform" data-webform-class=".multiomyx-brochure" href="#">Access</a></p> </div> </div> </div> </div> </div> </div> <div id="" class="paragraph paragraph--type--adjustable-image-content paragraph--view-mode--default"> <div class="adjustable-image-section img-left"> <div class="adjustable-image-content"> <div class="media-wrap"> <div class="field field--name-field-media field--type-entity-reference field--label-hidden field__item"><article class="media media--type-image media--view-mode-default"> <div class="field--name-field-media-image"> <img class="lazyload-img" data-lazyload="/sites/default/files/2021-06/MultiOmyx-Immune-Cell-Figure.jpg" src="/sites/default/files/styles/lazy_load/public/2021-06/MultiOmyx-Immune-Cell-Figure.jpg?itok=e5yQDP6E" alt="MultiOmyx Immune Cell Figure" height="484" width="872" /> </div> </article> </div> </div> <div class="content-wrap"> <h3 class="field--name-field-title">Comprehensive immunophenotyping from a single slide</h3> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><p>Immunotherapy is a cancer treatment approach that in recent years has resulted in great clinical success. In order to develop effective precision immunotherapies, it is important to identify immune checkpoint targets and the types and locations of immune cells in the tumor microenvironment; e.g., B cells, T cells, Tumor-Associated Macrophages (TAMs), and others. MultiOmyx<sup>™</sup> Tumor Infiltrating Lymphocyte Panels provide the ideal solution – quantitative analytical results from every cell on your single FFPE slide.</p> </div> </div> </div> </div> </div> <div class="paragraph paragraph--type--resources paragraph--view-mode--default"> <h4 class="resources-title">Resources</h4> <div class="field field--name-field-links field--type-link field--label-hidden field__items"> <div class="field__item"> <a href="/sites/default/files/2023-11/MultiOmyx-Fact-Sheet_rev102621.pdf" tabindex="0" target="_blank"> MultiOmyx™ Fact Sheet </a> </div> <div class="field__item"> <a href="/sites/default/files/2021-07/Spotlight_on_Sponsor_Infinity_030401_Final.pdf" tabindex="0" target="_blank"> MultiOmyx™ Case Study </a> </div> <div class="field__item"> <a href="/events/webinars/perivascular-accumulation-immunosuppressive-cells-stroma-human-tumors" tabindex="0" > MultiOmyx™ OnDemand Webinar </a> </div> </div> </div> <div id="" class="paragraph paragraph--type--text-image-equal-height-columns paragraph--view-mode--default corporate-pattern-bg"> <div class="equal-height-image-section img-right"> <div class="equal-height-image-content"> <div id="paragraph-id-625" class="media-wrap lazyload-bg" style=";background-image: url('/sites/default/files/styles/lazy_load/public/2021-06/MultiOmyx-Image.jpg?itok=5uXRx3WZ');" data-lazyload="/sites/default/files/2021-06/MultiOmyx-Image.jpg"></div> <div class="content-wrap" style=""> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><h2>MultiOmyx<sup>™</sup> also offers:</h2> <ul> <li>Unambiguous immune cell co-expression and co-localization</li> <li>Advanced quantitative cellular classification</li> <li>Medical, scientific, & bioinformatics consultation</li> <li>Custom assay design & verification</li> <li>Advanced image analysis & visualization tools</li> <li>Spatial analytics</li> <li>Integrated with FISH, RNAScope, and NGS</li> <li>High order: >= 7-plex</li> <li>Comprehensive analysis of tumor biology</li> <li>Monitoring of the immune microenvironment for the characterization of tumors pre- and post- I/O treatment</li> <li>Comprehensive Immunophenotyping at single cell level from one FFPE slide</li> </ul> </div> </div> </div> </div> </div> <div id="" class="paragraph paragraph--type--wysiwyg-full-width paragraph--view-mode--default corporate-pattern-bg"> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><table border="1" cellpadding="1" cellspacing="1"> <thead> <tr> <th scope="col">Test</th> <th scope="col">Slide Requirements</th> <th scope="col">Requirements</th> </tr> </thead> <tbody> <tr> <td><strong>MultiOmyx™</strong></td> <td>Duplicate slides per sample, cut at thickness of 3-5 microns.</td> <td>Transport of paraffin blocks and unstained slides are at room temperature. <strong>Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.</strong></td> </tr> </tbody> </table> <p>Specimen requirements for tumor tissue: FFPE tissue: Paraffin block is preferred, slide preferences are listed above</p> <p>Storage and Transportation: Use cold pack for transporting block and/or blood, making sure cold pack is not in direct contact with specimen. Peripheral Blood: Ship same day as drawn whenever possible; specimens <72 hours old.</p> </div> </div> <div class="paragraph paragraph--type--columns paragraph--view-mode--default corporate-pattern-bg"> <div id="" class="col-full padding-top-100"> <div class="field field--name-field-column-content field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <div class="paragraph paragraph--type--wysiwyg paragraph--view-mode--default"> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><p class="medium">MultiOmyx<sup>™</sup> Technologies & Applications</p> </div> </div> </div> </div> </div> </div> <div id="" class="paragraph paragraph--type--accordion paragraph--view-mode--default"> <div class="field field--name-field-accordion-items field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <div class="paragraph paragraph--type--accordion-item paragraph--view-mode--default"> <div class="field field--name-field-title field--type-string field--label-hidden field__item">Technologies</div> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><h2>MultiOmyx<sup>™</sup> Hyperplexed Immunofluorescence Assay</h2> <h4>Analyze Up to 60 Stains on a Single Slide</h4> <p>MultiOmyx™ assay has similar staining characteristics as standard immunohistochemical stains, and has the significant advantage that multiple proteins demonstrated up to 60 can be interrogated from a single FFPE section1. Direct comparison of multiple biomarkers is made on the same cell, enabling routine co-expression analysis and identification of cells requiring multiple biomarkers staining (e.g., Tregulatory CD3+CD4+FoxP3+).</p> <p> </p> <article class="align-center media media--type-image media--view-mode-default"> <div class="field--name-field-media-image"> <img class="lazyload-img" data-lazyload="/sites/default/files/2021-07/MultiOmyx-Hyperplexed-Immunofluorescence-Assay.png" src="/sites/default/files/styles/lazy_load/public/2021-07/MultiOmyx-Hyperplexed-Immunofluorescence-Assay.png?itok=7hhXE9YS" alt="MultiOmyx Hyperplexed Immunofluorescence Assay" height="299" width="961"> </div> </article> </div> </div> </div> <div class="field__item"> <div class="paragraph paragraph--type--accordion-item paragraph--view-mode--default"> <div class="field field--name-field-title field--type-string field--label-hidden field__item">Applications</div> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><h2>Immune Cells</h2> <h4>Unambiguous Classification of Immune Cell Phenotypes</h4> <p>MultiOmyx<sup>™</sup> technology allows for direct comparison of multiple biomarkers on the same cell, enabling unambiguous classification of immune cell phenotypes to differentiate T cells, B cells, NK cells, macrophages, and dendritic cells in the tumor microenvironment. Without being restricted to a handful of markers, MultiOmyx<sup>™</sup> can further differentiate functionally, activated immune cells from suppressed immune cells in context to tumor, stroma, and tumor margins.</p> <h3>Functional Differentiation of T<sub>cytotoxic</sub></h3> <article class="align-center media media--type-image media--view-mode-default"> <div class="field--name-field-media-image"> <img class="lazyload-img" data-lazyload="/sites/default/files/2021-06/Functional-Differentiation-of-Tcytotoxic.jpg" src="/sites/default/files/styles/lazy_load/public/2021-06/Functional-Differentiation-of-Tcytotoxic.jpg?itok=mFhB5-kU" alt="Functional Differentiation of Tcytotoxic" height="669" width="835"> </div> </article> <p>MultiOmyx staining was performed on CD3, CD4, CD8, CD45RO, GRANZYMe B, PD1, and Ki67 to identify memory T<sub>cytotoxic</sub> (CD3+CD4-CD8+CD45RO+), effector T<sub>cytotoxic</sub> (CD3+CD4-CD8+Granzyme B+), anergic T<sub>cytotoxic</sub> (CD3+CD4-CD8+PD1+) and proliferating T<sub>cytotoxic</sub>(CD3+CD4-CD8+Ki67+) cells. Arrows indicate T<sub>cytotoxic</sub> cells representing each of the four functional classifications in their respective images.</p> </div> </div> </div> </div> </div> <div class="paragraph paragraph--type--columns paragraph--view-mode--default corporate-pattern-bg"> <div id="" class="col-full padding-top-100"> <div class="field field--name-field-column-content field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <div class="paragraph paragraph--type--wysiwyg paragraph--view-mode--default"> <div class="clearfix text-formatted field field--name-field-body field--type-text-long field--label-hidden field__item"><p class="medium text-align-center">Latest MultiOmyx<sup>™</sup> News</p> </div> </div> </div> </div> </div> </div> <div class="paragraph paragraph--type--latest-literature paragraph--view-mode--default"> <div class="views-element-container"><div class="latest-literature view view-newsroom view-id-newsroom view-display-id-block_5 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fa-cloud-download"></div> <div class="item-text small-upper">Download</div> </a></div> </div> </div></div> <div class="views-row"><div class="newsroom-item-wrap"> <div class="item-content-wrap"> <div class="content-title"><p class="medium">Targeting a STING agonist to perivascular macrophages in prostate tumors delays resistance to androgen deprivation therapy</p></div> <div class="content-more-info">Journal for ImmunoTherapy of Cancer</div> </div> <div class="detail-wrap"> <div class="detail-date"><b><div class="label-date small-upper">Presented</div>Jul 10, 2024</b></div> <div class="detail-type"><b><div class="label-type small-upper">Type</div>Publication</b></div> </div> <div class="file-wrap"> <div class="file-link"><a href="https://jitc.bmj.com/content/jitc/12/7/e009368.full.pdf" target="_blank" class="small-upper"> <div class="item-icon read-more fal fa-cloud-download"></div> <div class="item-text">Learn More</div> </a></div> </div> </div></div> <div class="views-row"><div 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