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data-hx-get="https://scipio.bio/index.php?p=actions/sprig-core/components/render" data-hx-vals="{"selectedCategory":"new-to-single-cell"}" data-sprig-parsed> New to single-cell (0) </button> </div> </div> </div> </section> <section class="list"> <div class="inner"> <div class="faq-accordion accordion-container" id="results"> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">What sample types can I process with the Asteria™ single-cell RNAseq Kit?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>Currently, the Asteria™ single-cell RNAseq kit has been validated to process mammalian cells, including primary and cultured cell lines.</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">Can I stop the experiment and resume later? If so, how long after I started processing my sample?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>Yes, the Asteria™ single-cell RNA seq kit features a stopping point 2 hours after starting the protocol. The sample can then be safely stored at -80 °C for three days without loss of transcriptomic information. Stability for a longer period is being tested and further data will be made available soon.</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">Which library preparation kit(s) is recommended to prepare my cDNA for sequencing?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>We validated using the NEBNext ® Ultra II FS DNA Library Prep kit from New England Biolabs with certain adaptations described in the user guide of Asteria for optimal sequencing results. We will expand this list as we validate other library preparation kits.</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">Do I need lab equipement for the Asteria™ single-cell RNA sequencing kit?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>The use of Asteria™ single-cell RNA seq kit only requires basic cell and molecular biology laboratory equipment. A shortlist is available below,</p> <p>please refer to the User Guide for more details.</p> <p>• Heat block</p> <p>• PCR thermocycler</p> <p>• Centrifuge</p> <p>• Cell counting equipment (microscope and C-chips from Nanotek are recommended)</p> <p>• Orbital shaker</p> <p>• Benchtop centrifuge for 1.5 mL microtubes</p> <p>• Mini-centrifuge for 1.5 mL microtubes</p> <p>• Rotator/wheel for 1.5 mL microtubes</p> <p>• Calibrated Micropipettes (P10, P20, P200, P1000)</p> <p>• Vortex mixer</p> <p>The use of the kit requires the following consumables and reagents:</p> <p>• Sterile serological pipets (5, 10 and 25 mL)</p> <p>• Sterile and nuclease-free micropipette filtered tips (low-retention tips are recommended)</p> <p>• DNase/RNase-free 1.5 mL microtubes</p> <p>• DNase/RNase-free PCR strips</p> <p>• Molecular biology grade DNase/RNase-free water</p> <p>• 0.1M NaOH solution (freshly prepared from Molecular biology grade stock solution)</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">How many input cells can I use in a sample preparation experiment?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>The current protocol is designed to start with 10,000 to 15,000 input cells per sample. The current kit provides enough reagents to label a large number of cells and to run 4 such samples.</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">Where can I order the Asteria™ single-cell RNA sequencing kit?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>We invite you to find the contact of your local distributor on this page. If no distributor yet covers your country, we will deliver the kit to you ourselves and we invite you to fill out our contact form here.</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">How can I access the Cytonaut™ cloud-based analysis software?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>Everyone can access the Cytonaut™ software by creating a free account. By default, you will be able to explore demo data in read-only mode to play with the features of the software. If you want to run your own data, at the moment you will need to purchase the Asteria™ Single-Cell RNA sequencing kit to generate your data.</p></p> </div> </div> <div class="ac"> <h2 class="ac-header"> <button type="button" class="ac-trigger">Do I need to download a software or packages on my computer to use Cytonaut™?</button> </h2> <div class="ac-panel"> <p class="ac-text"><p>Cytonaut™ is entirely cloud-based, meaning the entire analysis is managed through our web interface. You can access your analysis from anywhere, and the computational analysis is run remotely.</p></p> </div> </div> </div> </div> </section> </div> </div> </main> <footer> <div class="inner"> <div class="top"> <div class="logo"> <img class="img header__logo" src="https://scipio.bio/dist/src/images/logo/logo-white.svg" alt="Scipio Science Logo" width="206" height="80" fetchpriority="high" decoding="async" loading="eager"> </div> <div class="social"> <ul> <li> <a target="_blank" href="https://fr.linkedin.com/company/scipio-bioscience"><img src="https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/logos/linkedin-in.svg"/></a> </li> <li> <a target="_blank" href="https://youtube.com/@scipiobioscience4275?feature=shared"><img src="https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/logos/youtube.svg"/></a> </li> </ul> </div> <nav aria-labelledby="footer-menu"> <ul> <li> <a href="https://scipio.bio/products/asteria"> Asteria™ </a> </li> <li> <a href="https://scipio.bio/products/cytonaut"> Cytonaut™ </a> </li> <li> <a href="https://scipio.bio/supports"> Resource Center </a> </li> <li> <a href="https://scipio.bio/single-cell-company"> Company </a> </li> <li> <a href="https://scipio.bio/careers"> Careers </a> </li> <li> <a href="https://scipio.bio/contact-us"> Contact </a> </li> </ul> </nav> </div> <div class="legal"> <div class="copyright"> <span>@2025 Scipio bioscience All rights reserved.</span> </div> <div class="corpo"> <ul> <li> <a href="/legal-notices">Legal Notices</a> </li> <ul></div> <div class="made_by"> by <a href="https://leeroy.ca" target="_blank">LEEROY</a> </div> </div> </div> </footer> </div> </div> <script type="application/ld+json">{"@context":"http://schema.org","@graph":[{"@type":"WebPage","author":{"@id":"#identity"},"copyrightHolder":{"@id":"#identity"},"copyrightYear":"2023","creator":{"@id":"#creator"},"dateCreated":"2023-12-11T13:15:56-08:00","dateModified":"2024-06-17T10:26:10-07:00","datePublished":"2023-12-11T13:15:00-08:00","description":"Explore our single-cell FAQ for more information about our products and services.","headline":"FAQ","image":{"@type":"ImageObject","url":"https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/_1200x630_crop_center-center_82_none/First_Section.jpg?mtime=1706648949"},"inLanguage":"en-us","mainEntityOfPage":"https://scipio.bio/faq","name":"FAQ","publisher":{"@id":"#creator"},"url":"https://scipio.bio/faq"},{"@id":"#identity","@type":"LocalBusiness","name":"Scipio bioscience","priceRange":"$"},{"@id":"#creator","@type":"Organization"},{"@type":"BreadcrumbList","description":"Breadcrumbs list","itemListElement":[{"@type":"ListItem","item":"https://scipio.bio","name":"Home","position":1},{"@type":"ListItem","item":"https://scipio.bio/faq","name":"FAQ","position":2}],"name":"Breadcrumbs"}]}</script><script src="https://scipio.bio/cpresources/2fc79849/htmx.min.js?v=1718647167"></script> <script>htmx.on('htmx:afterSwap', function(event) { htmx.trigger('#faqComponent', 'sprig-refresh'); });</script></body> </html>