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Easier</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-3066 post type-post status-publish format-standard has-post-thumbnail category-connect-to-science category-general entry" aria-label="6 Tested Technology Combinations that Can Make Life in the Lab Easier"><header class="entry-header"><h1 class="entry-title">6 Tested Technology Combinations that Can Make Life in the Lab Easier</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/danadamico/" title="Posts by Dana D'Amico" rel="author">Dana D'Amico</a> </span> <span class="post-info-date"> 07.02.2024 </span> </header><div class="entry-content"><p><img decoding="async" class="aligncenter size-full wp-image-3138" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/PerfectPairingsBlog.png" alt="" width="1280" height="720" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/PerfectPairingsBlog.png 1280w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/PerfectPairingsBlog-300x169.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/PerfectPairingsBlog-1024x576.png 1024w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/PerfectPairingsBlog-768x432.png 768w" sizes="(max-width: 1280px) 100vw, 1280px" /></p> <h2></h2> <h2><em>We asked our technical experts for their favorite product pairings at the bench.</em></h2> <p>Some things just belong together: coffee and cream, lock and key, thunder and lightning, antibodies and antigens.</p> <p>We asked our technical experts about their favorite lab tool combinations across applications, from molecular biology to cell therapy.</p> <h3><strong>1. High-throughput, high-fidel</strong><strong>ity PCR </strong></h3> <p>We’re kicking off our list with an unlikely pair of ideas: fast <em>and</em> accurate.</p> <p>Too often in life, you can do something quickly or you can do it correctly – but not both.</p> <p>When it comes to nucleic acid analysis, sequence accuracy is often non-negotiable. This is certainly true for applications like next-generation sequencing (NGS), mutagenesis, cloning, sequencing, or single nucleotide polymorphism (SNP) analysis.</p> <p>One of our experts’ go-to recommendations for preserving sequence accuracy is <a href="https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SPlatSuperFi">Platinum SuperFi II DNA Polymerase</a>, which can achieve >300x the fidelity of <em>Taq </em>DNA polymerase in NGS applications. Hot-start technology inhibits enzyme activity until after denaturation, which helps to prevent nonspecific amplification and primer degradation.</p> <div id="attachment_3139" style="width: 634px" class="wp-caption aligncenter"><img decoding="async" aria-describedby="caption-attachment-3139" class="size-full wp-image-3139" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hifi.png" alt="Fidelity comparison across commercially available enzymes relative to Taq enzyme. A 3.9 kb sequence was amplified by PCR using different DNA polymerases, and the resulting PCR amplicons were then fragmented with a MuA transposase. Unique molecular identifiers (UMI), which consist of 12 random nucleotides, were introduced during fragmentation to individually tag each product. After next-generation sequencing, reads were aligned to the correct sequence, grouped by UMI families, and errors were called. Errors were identified only if they were present in all reads in the UMI family; otherwise they were discarded as sequencing errors. The polymerase fidelities were normalized to the Taq DNA polymerase. Read research article here." width="624" height="204" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hifi.png 624w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hifi-300x98.png 300w" sizes="(max-width: 624px) 100vw, 624px" /><p id="caption-attachment-3139" class="wp-caption-text">Fidelity comparison across commercially available enzymes relative to Taq enzyme. A 3.9 kb sequence was amplified by PCR using different DNA polymerases, and the resulting PCR amplicons were then fragmented with a MuA transposase. Unique molecular identifiers (UMI), which consist of 12 random nucleotides, were introduced during fragmentation to individually tag each product. After next-generation sequencing, reads were aligned to the correct sequence, grouped by UMI families, and errors were called. Errors were identified only if they were present in all reads in the UMI family; otherwise they were discarded as sequencing errors. The polymerase fidelities were normalized to the Taq DNA polymerase. Read research article here.</p></div> <p> </p> <p>The speed comes into play when a high-fidelity polymerase is combined with a <a href="https://www.thermofisher.com/us/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/proflex-pcr-system.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SProFlex">high-throughput PCR system</a> that can run up to three independent experiments simultaneously or high-throughput runs with dual 96 or 384 well-blocks.</p> <div id="attachment_3140" style="width: 350px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3140" class="size-full wp-image-3140" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/proflex.png" alt="ProFlex PCR System with the 3 x 32-well block for running up to three independent experiments simultaneously." width="340" height="235" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/proflex.png 340w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/proflex-300x207.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/proflex-96x65.png 96w" sizes="(max-width: 340px) 100vw, 340px" /><p id="caption-attachment-3140" class="wp-caption-text">ProFlex PCR System with the 3 x 32-well block for running up to three independent experiments simultaneously.</p></div> <p> </p> <p>With this combination of speed and accuracy, you can have your PCR cake and eat it too (just not literally!).</p> <h4><strong>Our experts recommend: </strong></h4> <ul> <li><a href="https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SPlatSuperFi">Platium SuperFi II DNA Polymerase</a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/proflex-pcr-system.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SProFlex">ProFlex PCR System</a></li> </ul> <p> </p> <h3><strong>2. Discovery, development, or manufacturing of nucleic acid therapeutics </strong></h3> <p>In a post- SARS-CoV-2 world, nucleic acid therapeutics continue to gain steam as a technology with endless possibilities. But the monumental achievement of the world’s first mRNA vaccinations owes success to another decades-in-the-making technology focused on optimized lipid shells.</p> <div id="attachment_3141" style="width: 634px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3141" class="size-full wp-image-3141" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/mrnagraphic.png" alt="mRNA production involves template linearization, in vitro transcription, DNA removal, and post-transcriptional modification. Below is a summary of many of the materials typically used in mRNA production." width="624" height="223" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/mrnagraphic.png 624w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/mrnagraphic-300x107.png 300w" sizes="(max-width: 624px) 100vw, 624px" /><p id="caption-attachment-3141" class="wp-caption-text">mRNA production involves template linearization, in vitro transcription, DNA removal, and post-transcriptional modification. Below is a summary of many of the materials typically used in mRNA production.</p></div> <p>The first COVID-19 vaccines successfully used liquid nanoparticles (LNPs) as the key molecular delivery vehicles for therapeutic mRNA<em>. </em>LNPs are one of the most successful <em>in vivo</em> nano-delivery tools in chemotherapy and cell therapies, antibiotic treatment, and nucleic acid therapeutics today. Like liposomes, LNPs encapsulate a drug to allow controlled access to target cells and tissues.</p> <div id="attachment_3142" style="width: 686px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3142" class="size-full wp-image-3142" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/invivo.png" alt="Overview of in vivo transfection using Invivofectamine 3.0 reagent. LNP-siRNA complexes are injected into the organism, effectively delivering the RNAi payload to target cells." width="676" height="595" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/invivo.png 676w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/invivo-300x264.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/invivo-310x274.png 310w" sizes="(max-width: 676px) 100vw, 676px" /><p id="caption-attachment-3142" class="wp-caption-text">Overview of in vivo transfection using Invivofectamine 3.0 reagent. LNP-siRNA complexes are injected into the organism, effectively delivering the RNAi payload to target cells.</p></div> <p>Our technical experts can help enable <a href="https://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/nucleic-acid-therapeutic-development-solutions.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SNATx">nucleic acid R&D</a> from both angles with a robust <a href="https://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/nucleic-acid-therapeutic-development-solutions/mrna-therapeutic-resources/mrna-synthesis-reagents.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SmRNASynth">portfolio</a> of enzymes, nucleotides, and amidites (TheraPure) and industry-leading <a href="https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/applications/in-vivo-transfection.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SInVivoTransfec">LNP-based <em>in vivo </em>transfection technologies</a> like <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-reagents.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2STransfecReagent">Lipofectamine and Invivofectamine reagents</a>.</p> <h4><strong>Our experts recommend: </strong></h4> <ul> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-reagents.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2STransfecReagent">Lipid-mediated transfection reagents</a></li> <li><a href="https://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/nucleic-acid-therapeutic-development-solutions/mrna-therapeutic-resources/mrna-synthesis-reagents.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SmRNASynth">GMP-quality mRNA enzymes, nucleotides, and amidites</a></li> </ul> <p> </p> <h3><strong>3. Cell culture </strong></h3> <p>Foundational techniques like cell culture represent the base of your research workflow; with weak materials, it could all fall apart from the bottom up.</p> <p>When culture tools like plastics, media, FBS, supplements, and cell lines are validated together, researchers can have confidence in holistic, system-wide product performance and reproducibility.</p> <p>For this reason, R&D experts have created a variety of <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-workflow-solutions.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCBSelectionTool#selection-tools">digital selection tools</a> to help researchers find the most optimized pairings of culture materials, down to the <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-workflow-solutions/cell-culture-select-tool.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellCultureSel">ideal product mix recommended by cell line.</a></p> <div id="attachment_3143" style="width: 634px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3143" class="size-full wp-image-3143" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/cellsmcf7.png" alt="Representative images of MCF7 cells grown in Gibco DMEM supplemented with 10% Gibco FBS, 10 µg/ml human Insulin and 1% Penicillin-Streptomycin (optional). Nunclon Delta 75cm2 flasks were used for cell culture. Images were captured on EVOS M7000 microscope under 10X magnification for low confluency (left) and high confluency (right) images." width="624" height="235" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/cellsmcf7.png 624w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/cellsmcf7-300x113.png 300w" sizes="(max-width: 624px) 100vw, 624px" /><p id="caption-attachment-3143" class="wp-caption-text">Representative images of MCF7 cells grown in Gibco DMEM supplemented with 10% Gibco FBS, 10 µg/ml human Insulin and 1% Penicillin-Streptomycin (optional). Nunclon Delta 75cm2 flasks were used for cell culture. Images were captured on EVOS M7000 microscope under 10X magnification for low confluency (left) and high confluency (right) images.</p></div> <p>Likewise, if there ever was a “power trio” of iconic cell culture basics to recommend, it would be <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-media.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCCMedia">Gibco cell culture media</a> and <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/fbs.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SFBS">FBS</a> and <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/cell-culture-plastics.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCulturePlastics">Thermo Scientific Nunc cell culture plastics</a>. Researchers will also find options for bench-stable, pre-aliquoted, and more <a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-workflow-solutions/cell-culture-products-designed-sustainability.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SSustCellCult">sustainably manufactured media</a>.</p> <h4><strong>Our experts recommend: </strong></h4> <ul> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-media.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCCMedia">Gibco cell culture media</a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/fbs.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SFBS">Gibco FBS</a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-culture/cell-culture-plastics.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCulturePlastics">Thermo Scientific Nunc cell culture plastics</a></li> </ul> <p> </p> <h3><strong>4. Cell painting and single-cell analysis</strong></h3> <p><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellImageHCS">High-content screening (HCS)</a>, also known as high-content analysis, is a method of evaluating the phenotype of individual cells or cellular components – aspects like changes in size, shape, organelle content, protein expression levels, and more. It is central to drug discovery and other research questions seeking to identify small molecules like nucleic acids or peptides that can alter the cell in a particular way.</p> <p><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/cell-painting-assay.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellPainting">Cell painting</a> is an advanced HCS technique capable of quantifying changes in cellular structures in drug discovery or safety screenings. With cell painting, a researcher can visualize the spatial organization of subcellular components, paving the way towards insights into protein organization, cell viability, cell proliferation and toxicity, DNA damage and more.</p> <div id="attachment_3144" style="width: 725px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3144" class=" wp-image-3144" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hcshx.png" alt="Representative image and data readout from an HCS data set acquired on a Cellinsight CX7 LZR Pro system." width="715" height="370" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hcshx.png 887w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hcshx-300x155.png 300w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/hcshx-768x397.png 768w" sizes="(max-width: 715px) 100vw, 715px" /><p id="caption-attachment-3144" class="wp-caption-text">Representative image and data readout from an HCS data set acquired on a Cellinsight CX7 LZR Pro system.</p></div> <p>With a combination of the right <a href="https://www.thermofisher.com/us/en/home/brands/molecular-probes/key-molecular-probes-products/alexa-fluor.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SAlexaFluor">Invitrogen Alexa Fluor Dyes</a> or <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellPaintRsrch#cell-painting-research">Invitrogen Image-iT Cell Painting Kit</a> and the <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx7-lzr.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCX7LZR">CellInsight CX7 LZR Pro HCS</a>, scientists have produced stunning images worthy of <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SConfocalImaging#publication-quality-confocal-imaging">framing after use</a>. An <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/accessories.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SHCAIncubator#invitrogen-hca-onstage-incubator">onstage incubator</a> also opens the possibility of analyzing live 2D or 3D cell samples over time.</p> <p><img loading="lazy" decoding="async" class="aligncenter wp-image-3145" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/cx7cells.png" alt="" width="717" height="387" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/cx7cells.png 624w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/cx7cells-300x162.png 300w" sizes="(max-width: 717px) 100vw, 717px" /></p> <p>A cell painting kit is ideal for researchers looking for a more convenient high-throughput workflow; the kit provides pre-measured fluorescent conjugates for multi-well plate experiments, validated using reagents based on those noted in the highly cited <a href="https://pubmed.ncbi.nlm.nih.gov/27560178/"><em>Nature Protocols </em>cell painting paper</a> that first described the technique in 2016.</p> <h4><strong>Our experts recommend: </strong></h4> <ul> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx7-lzr.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCX7LZR">CellInsight CX7 LZR Pro HCS / HCA</a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SConfocalImaging#publication-quality-confocal-imaging">Invitrogen Image-iT Cell Painting Kit</a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/accessories.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SHCAIncubator#invitrogen-hca-onstage-incubator">Invitrogen HCA Onstage Incubator</a></li> <li><a href="https://www.thermofisher.com/us/en/home/brands/molecular-probes/key-molecular-probes-products/alexa-fluor.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SAlexaFluor">Invitrogen Alexa Fluor Dyes</a></li> </ul> <p> </p> <h3><strong>5. Cell therapy manufacturing</strong></h3> <p><strong> </strong><br /> One of today’s biggest boons in oncology is also one of its biggest challenges – autologous CAR T-cell therapies can effectively cure some of the toughest blood cancers today, but the GMP manufacturing process for these personalized therapies is complex, lengthy, and a bottleneck to access for many.</p> <p>A closed, modular system is designed to minimize contamination and uphold the highest GMP standards of quality and safety, while allowing researchers the flexibility to automate and streamline their process.</p> <p>The <a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-manufacturing-solutions/rotea-counterflow-centrifugation-system.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SRotea">Gibco CTS Rotea Counterflow Centrifugation System, </a> <a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-manufacturing-solutions/xenon-electroporation-system.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SXenon">Gibco CTS Xenon Electroporation System, </a> and <a href="https://www.thermofisher.com/us/en/home/life-science/bioproduction/gibco-bioprocessing/magnetic-separation-system.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SDynacellect">Gibco CTS DynaCellect Magnetic Separation System</a> form a trio of disruptive cell therapy manufacturing innovations that can be combined for even greater effect.</p> <div id="attachment_3146" style="width: 730px" class="wp-caption aligncenter"><a href="https://www.thermofisher.com/us/en/home/virtual/t-cell-manufacturing-lab-3d-tour.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellTheraLab"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3146" class="wp-image-3146 size-full" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/Untitled-7.png" alt="Explore the Virtual T Cell Therapy Manufacturing Lab. Take a virtual tour of an automated cell therapy manufacturing lab and explore tools for T cell manufacturing, lentiviral production, and analytics. In the cell processing and engineering area, the CTS DynaCellect Magnetic Separation System uses magnetic bead technology for target-cell separation. From there, the CTS Rotea cell processing instrument uses counterflow centrifugation to concentrate, wash, or exchange buffers in a closed, automated system. Lastly, the CTS Xenon electroporation system enables closed, scalable electroporation for large-scale nonviral delivery and GMP-compliant cell therapy manufacturing. " width="720" height="504" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/Untitled-7.png 720w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/Untitled-7-300x210.png 300w" sizes="(max-width: 720px) 100vw, 720px" /></a><p id="caption-attachment-3146" class="wp-caption-text">Explore the <a href="https://www.thermofisher.com/us/en/home/virtual/t-cell-manufacturing-lab-3d-tour.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellTheraLab">Virtual T Cell Therapy Manufacturing Lab</a>. Take a virtual tour of an automated cell therapy manufacturing lab and explore tools for T cell manufacturing, lentiviral production, and analytics. In the cell processing and engineering area, the CTS DynaCellect Magnetic Separation System uses magnetic bead technology for target-cell separation. From there, the CTS Rotea cell processing instrument uses counterflow centrifugation to concentrate, wash, or exchange buffers in a closed, automated system. Lastly, the CTS Xenon electroporation system enables closed, scalable electroporation for large-scale nonviral delivery and GMP-compliant cell therapy manufacturing.</p></div> <p>Researchers can kick off cell processing and engineering with the CTS DynaCellect, which earned an R&D 100 Award in 2023 for an innovative closed, sterile design that allows GMP-compliant cell isolation.</p> <p>The CTS Rotea system is another innovative tool, the <a href="https://www.thermofisher.com/blog/life-in-the-lab/qa-with-cell-biologist-and-gibco-cts-rotea-collaborator-rebecca-lim/?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SRebeccaLimInt">product of serendipitous academic-industry collaboration .</a> It was born in part out of a researcher’s need to <a href="https://www.thermofisher.com/blog/life-in-the-lab/qa-with-cell-biologist-and-gibco-cts-rotea-collaborator-rebecca-lim/?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SRebeccaLimInt">process and buffer the cell samples of extremely premature infants</a> (<500 grams at birth!) within the small confines of a biological safety cabinet.</p> <p>From there, the CTS Xenon electroporation system offers a gentler method of scalable, customizable nonviral transfection for genetic modification of cells. Like the CTS Rotea system, the CTS Xenon electroporation system was designed in <a href="https://www.thermofisher.com/blog/life-in-the-lab/customer-led-innovation-building-the-cts-xenon/?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCustomerInnoXen">close collaboration with customer-scientists</a> around the world during pandemic quarantine.</p> <p>By combining these three novel instruments and <a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-systems.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellTheraSystem">reliable specialized reagents</a>, a researcher in the cell therapy space can efficiently isolate and enrich a target cell population and then introduce specific genetic modifications or therapeutic genes into the cells.</p> <h4><strong>Our experts recommend: </strong></h4> <ul> <li><a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-manufacturing-solutions.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellTheraManu">Gibco Cell Therapy Manufacturing Tools</a></li> <li><a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-systems.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SCellTheraSystem">Gibco Cell Therapy Systems (CTS) Products<br /> </a></li> </ul> <h3></h3> <h3></h3> <h3><strong>Automated sample preparation </strong></h3> <p>There are a <a href="https://www.thermofisher.com/blog/life-in-the-lab/6-reasons-to-automate-your-sample-preparation-workflows/?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SSPAutomationBlg">handful of reasons</a> why labs today are increasingly deciding to automate their sample preparation processes: time savings and scale-up, lower error margins and better reproducibility, improved safety, easy customizability, streamlined staff size, reduced cost and waste, and more.</p> <div id="attachment_3147" style="width: 634px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-3147" class="size-full wp-image-3147" src="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/spchart.png" alt="Automated extraction of nucleic acid, cells, exosomes and proteins can be achieved with either manual or automated laboratory workflows. Academic labs may choose manual workflows for educational research, while biotech companies may use manual workflows for initial validation and transition to automated instruments when scaling up their study or research products." width="624" height="578" srcset="https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/spchart.png 624w, https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2024/07/spchart-300x278.png 300w" sizes="(max-width: 624px) 100vw, 624px" /><p id="caption-attachment-3147" class="wp-caption-text">Automated extraction of nucleic acid, cells, exosomes and proteins can be achieved with either manual or automated laboratory workflows. Academic labs may choose manual workflows for educational research, while biotech companies may use manual workflows for initial validation and transition to automated instruments when scaling up their study or research products.</p></div> <p>There is a whole lineup of options for each unique sample preparation need, but the <a href="https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/advantages-automated-extraction.html.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SAutomatedExtrac">basic formula for success</a> that our technical experts recommend pairs an automated tool like the KingFisher purification systems with a corresponding magnetic bead reagent or kit (Invitrogen Dynabeads magnetic beads or Applied Biosystems MagMAX kits, depending on the sample type and target analyte).</p> <p>Hands-off, automated sample purification with magnetic bead technology has enabled discoveries in everything from <a href="https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/sample-preparation-solutions-cancer-research.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SSPCancerResrch">cancer research</a> to <a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/wastewater-monitoring-infectious-diseases-flyer.pdf?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SWastewater">wastewater surveillance</a> and <a href="https://www.youtube.com/watch?v=hbAPvkstcXU">microbiome research</a> to <a href="https://www.thermofisher.com/blog/life-in-the-lab/ancient-dna-sample-prep/?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SAncientDNA">paleogenomics</a> and <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SNextGenSeq">NGS</a>.</p> <h4><strong>Our experts recommend: </strong></h4> <ul> <li><a href="https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SKingfisher">Thermo Scientific KingFisher sample purification systems</a></li> <li><a href="https://www.thermofisher.com/us/en/home/brands/product-brand/dynal.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SDynabeads">Invitrogen Dynabeads magnetic beads</a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/automated-magmax-kits-nucleic-acid-extraction.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SMagMAX">Applied Biosystems MagMAX kits</a></li> </ul> <p> </p> <h2><strong>» </strong><strong><em> </em></strong><a href="https://www.thermofisher.com/us/en/home/global/forms/connect-to-science-digital-care-package.html?icid=bid_cbu_sbu_r01_co_cp1605_pjt12448_blg54884_0so_blg_il_awa_og_s00_C2SDigitalCarePkg"><strong><em>Subscribe to Connect to Science</em></strong></a><strong><em>, your slice of life science.</em></strong></h2> <p> </p> <p>##<br /> <em>© 2024 Thermo Fisher Scientific Inc. 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