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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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vertical-align: middle;"><h1 style="color: white;">Guide RNAs for CRISPR-Cas9</h1><p><a class="btn btn-featured" href="#selection-guide">Select gRNA formats</a></p></td></tr></tbody></table></div></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-2-column"><div class="container"><div class="row"><script> var showHideText = { 'seeRelatedText':'See Navigation', 'hideRelatedText':'Hide Navigation' }; </script><div class="span3 sidebar" role="complementary"><div id="link-leftnav-toggle-container"><a class='link-leftnav-toggle' href='#'>See Navigation</a></div><div class="container-leftnav"><div class="railInheritParsys iparsys parsys"><div class="railsection section"><div class="leftnav-standard"><div class="nav-list-header"><a href="/sg/en/home/life-science/genome-editing.html"><span class="nav-list-header-icon">&lsaquo;</span><span class="nav-list-header-text">Gene Editing</span></a></div><ul class="nav nav-list" id="navList"><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/cas9-nucleases.html">Cas9 Nucleases <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/invitrogen-truedesign-genome-editor.html">Invitrogen TrueDesign Genome Editor <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="active" id="navListActive"><a class="item-header" href="/sg/en/home/life-science/genome-editing/crispr-grna.html">CRISPR gRNAs <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/crispr-knock-in.html">Knock-in Editing and Gene Tagging <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/crispr-controls.html">CRISPR Controls <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/crispr-transfection.html">CRISPR Transfection <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/crispr-libraries.html">CRISPR Libraries <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/crispr-validation.html">TALEN and CRISPR Validation <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/talens.html">TALEN Gene Editing <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/crispr-nuclease-vector.html">CRISPR Nuclease Vectors <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/genome-editing-learning-center.html">Genome Editing Education: TALEN and CRISPR Resources <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/genome-editing/genome-editors.html">Genome Editing Cartoon <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/global/page-not-available.html">Everything you need for CRISPR success <span class="nav-list-arrow">&rsaquo;</span></a></li></ul><script> if ( $('#navListActive').children('ul').length > 0 ) { var activeNav = $j('#navListActive'); $j('#navList').prepend(activeNav); } </script></div></div><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="railInheritParsys iparsys parsys"></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="text parbase section"><h2>High-quality gRNAs for CRISPR-Cas9 and screening applications</h2><p>When using the CRISPR-Cas9 system to knock out gene expression or knock in a specific mutation, the design, production, and delivery of high-quality guide RNAs (gRNAs) are critical to success. Whether you need transfection-ready gRNAs to use with <a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-proteins.html">TrueCut Cas9 Proteins</a> or you need to harness <a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-lentivirus.html">lentivirus</a> to deliver your editing tools to hard-to-transfect or primary cells, we have you covered. Use our <a href="/sg/en/home/life-science/genome-editing/crispr-libraries/trueguide-grnas.html">gRNA gene search tool</a> to find predesigned gRNAs in either of these formats, or continue to our selection guide below for other choices.</p><p>For functional genomics screening, we’ve also packaged our advanced gRNA designs into <a href="/sg/en/home/life-science/genome-editing/crispr-libraries.html">CRISPR libraries</a> for high-throughput arrayed screening or economical pooled screening.</p></div><div class="text parbase section"><p><a class="cta-link" href="#selection-guide">Select from various CRISPR gRNA formats</a><br><a class="cta-link" href="#search-grnas">Discover quick tools to search and order gRNAs</a><br><a class="cta-link" href="#trueguide">Learn more about TrueGuide Synthetic gRNAs</a><br><a class="cta-link" href="#lentiarray">Learn more about LentiArray CRISPR gRNAs</a><br><a class="cta-link" href="#geneart">Learn more about IVT gRNAs</a></p></div><div class="heading section"><h2 id="selection-guide"><br>Selection guide to CRISPR gRNA formats </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-391208166"><tbody><tr><td width="13%"> </td><td width="29%" style="text-align: center;"><a href="#trueguide"><img src="/content/dam/LifeTech/global/life-sciences/images/grna-1.5.png" title="TrueGuide Synthetic gRNA" alt="Diagram of TrueGuide Synthetic gRNA"></a><br><b>TrueGuide Synthetic gRNA</b></td><td width="29%" style="text-align: center;"><a href="#lentiarray"><img src="/content/dam/LifeTech/global/life-sciences/images/grna-2.1.png" title="LentiArray Lentiviral gRNA" alt="Diagram of LentiArray Lentiviral gRNA"></a><br><b>LentiArray Lentiviral gRNA</b></td><td width="29%" style="text-align: center;"><a href="#geneart"><img src="/content/dam/LifeTech/global/life-sciences/images/grna-1.1.png" title="gRNA molecule" alt="Diagram of gRNA molecule"></a><br><b>Precision gRNA Synthesis Kit</b></td></tr><tr><td><b>Overview</b></td><td><ul><li>Choose from our catalog of ready-to-transfect <a href="/sg/en/home/life-science/genome-editing/crispr-libraries/trueguide-grnas.html">predesigned sgRNAs</a> for efficient knockout</li><li>Upload your custom sequence to our <a href="/order/custom-oligo/crispr">custom TrueGuide gRNA ordering tool</a></li><li>Design your custom edit with <a href="/sg/en/home/life-science/genome-editing/invitrogen-truedesign-genome-editor.html">TrueDesign Genome Editor</a></li></ul></td><td><ul><li>Choose your gRNA from our catalog of pre-designed gRNAs packaged as ready-to-use lentivirus</li><li>Available as individual constructs or <a href="/sg/en/home/life-science/genome-editing/crispr-libraries.html">screening libraries</a></li><li><a href="mailto:GEMServices@thermofisher.com?subject=Custom%20lentiviral%20CRISPR%20gRNA%20">Custom lentiviral services</a> are also available</li></ul></td><td><ul><li>A complete kit to generate ready-to-transfect gRNAs from a DNA template</li></ul></td></tr><tr><td><b>Format</b></td><td>Synthetic RNA oligo, chemically modified for stability</td><td>Lentiviral particles</td><td><i>In vitro-</i>transcribed (IVT) gRNA</td></tr><tr><td><b>Application</b></td><td>Knockout (predesigned) or knock-in (custom)</td><td>Knockout, including <a href="/sg/en/home/life-science/genome-editing/crispr-libraries.html">library screening</a></td><td>Knockout or knock-in</td></tr><tr><td><b>Species</b></td><td><ul><li>Human and mouse (predesigned)</li><li>All other species (custom)<a href="/sg/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries.html"></a></li></ul></td><td><ul><li>Human (predesigned)</li><li>All other species (custom)</li></ul></td><td><ul><li>All species</li></ul></td></tr><tr><td><b>Delivery method</b></td><td>Lipid-mediated transfection or electroporation</td><td>Lentiviral transduction</td><td>Lipid-mediated transfection or electroporation</td></tr><tr><td><b>Recommended Cas9 format</b></td><td><a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-proteins.html">TrueCut Cas9 Proteins</a></td><td><a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-lentivirus.html">LentiArray Cas9 Lentivirus</a></td><td><a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-proteins.html">TrueCut Cas9 Proteins</a></td></tr><tr><td><b>Controls</b></td><td><a href="/sg/en/home/life-science/genome-editing/crispr-controls.html">Positive and negative controls available</a></td><td><a href="/sg/en/home/life-science/genome-editing/crispr-controls.html">Positive and negative controls available</a></td><td> </td></tr><tr><td> </td><td style="text-align: center;"><a href="/sg/en/home/life-science/genome-editing/crispr-libraries/trueguide-grnas.html" class="btn btn-primary">Order now</a></td><td style="text-align: center;"><a href="/sg/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries/lentiarray-crispr-libraries/lentiarray-grnas.html" class="btn btn-primary">Order now</a></td><td style="text-align: center;"><a href="#order" class="btn btn-primary">Order now</a></td></tr></tbody></table></div></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2 id="search-grnas"> Quick tools to search and order gRNAs </h2></div><div class="heading section"><h3> Search for predesigned synthetic or lentiviral CRISPR sgRNAs </h3></div><div class="text parbase section"><p>TrueGuide Synthetic gRNAs are ready-to-transfect CRISPR single guide RNAs (sgRNAs) designed to provide specific and high-efficiency knockout of your target gene. If you need to enrich edited cells, are working with difficult-to-transfect cells, or need long-term sgRNA expression, use our LentiArray CRISPR gRNAs. </p></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/life-science/genome-editing/crispr-libraries/trueguide-grnas.html">Find predesigned TrueGuide gRNAs using our gene search tool</a><br><a class="cta-link" href="/sg/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries/lentiarray-crispr-libraries/lentiarray-grnas.html">Find predesigned LentiArray CRISPR gRNAs using our selection tool</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h3> Order a custom synthetic gRNA </h3></div><div class="text parbase section"><p>Have a design of your own? Whether it’s from a journal article, a colleague, or your favorite design tool, use our simple, fast interface to upload the custom gRNA sequences you would like synthesized into a <b>custom TrueGuide gRNA</b>. </p></div><div class="text parbase section"><p><a class="cta-link" href="/order/custom-oligo/crispr">Enter your own design with our TrueGuide custom gRNA ordering tool</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h3> Use our design tool to create a gene editing experiment and order your gRNAs </h3></div><div class="text parbase section"><p>Need design assistance for creating an engineered cell line? You can design your CRISPR gene knockout, knock-in, or tagging experiment, and order a complete set of reagents (including gRNAs) for it, with our free TrueDesign Genome Editor software.</p></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/life-science/genome-editing/invitrogen-truedesign-genome-editor.html">Design and order reagents for your entire CRISPR edit with the TrueDesign Genome Editor</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h3> Contact us for guide RNAs in plate format or other special orders </h3></div><div class="text parbase section"><p>To order more than 24 gRNAs for delivery on a 96-well plate, or for gRNAs for use with other nucleases or specific scaffold sequences, please contact us at <a href="mailto:GEMservices@thermofisher.com">GEMservices@thermofisher.com</a>.</p></div></div></div></div></div></div><div class="text parbase section"></div><div class="heading section"><h2 id="trueguide"><br>TrueGuide Synthetic gRNA for CRISPR </h2></div><div class="text parbase section"><p>Invitrogen TrueGuide Synthetic gRNAs are ready-to-transfect CRISPR guide RNAs designed by a proprietary algorithm for high-efficiency editing. These predesigned, guaranteed single guide RNAs offer targeted knockdown with high specificity across the human and mouse genomes.</p></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/life-science/genome-editing/crispr-libraries/trueguide-grnas.html">To get started, use the search tool above to search for your gene</a></p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table-1" id="table--1023540528"><tbody><tr><td> </td><td><center><img src="/content/dam/LifeTech/global/life-sciences/images/grna-1.5.png" alt="Diagram of TrueGuide Synthetic gRNA"></center><br><b>TrueGuide 1-piece modified Synthetic gRNA</b></td></tr><tr><td><b>Predesigned knockouts for mouse and human cells</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr><tr><td><b>Custom sequences</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr><tr><td><b>Validated controls available</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr><tr><td><b>Available in plate format</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr><tr><td><b>Ready-to-transfect</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr><tr><td><b>Chemical modifications*</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr><tr><td><b>Ideal for primary and stem cells</b></td><td style="text-align: center;"><span class="icon icon-ok"> </span></td></tr></tbody></table></div><p><small><span class="muted">*Chemical modifications include 2´O-Methyl analogs and phosphorothioate linkages to increase editing efficiency and protect against nuclease degradation.</span></small></p></div><div class="divider section"><hr /></div><div class="text parbase section"><ul class="the-icons"><li> <a class="cta-link" href="/sg/en/home/life-science/genome-editing/crispr-libraries/trueguide-grnas.html">Order now with our TrueGuide gRNA gene search tool</a></li><li><span class="icon-lt-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017058_TrueGuide_Synthetic_gRNA_UG.pdf">Download the TrueGuide Synthetic gRNA user guide</a></li></ul></div><div class="divider section"><hr /></div><div class="heading section"><h3> Data: High-efficiency editing with TrueGuide Synthetic gRNAs </h3></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_f1d1"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.320.low.jpg/1725389960766.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.320.medium.jpg/1725389960766.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.480.medium.jpg/1725389960766.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.620.high.jpg/1725389960766.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.full.high.jpg/1725389960766.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.full.high.jpg/1725389960766.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.320.high.jpg/1725389960766.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.480.high.jpg/1725389960766.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.320.medium.jpg/1725389960766.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.620.high.jpg/1725389960766.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.320.low.jpg/1725389960766.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_f1d1/image.img.480.medium.jpg/1725389960766.jpg" alt='Bar graph showing the cleavage efficiency of multiple kinase genes using TrueGuide Synthetic gRNAs' title='Cleavage efficiency of a CRISPR experiment using TrueGuide Synthetic gRNAs'></picture></div><style> #cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_f1d1 { max-width: 100%; } #cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_f1d1 img { width: 100%; max-width: 2250px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><small><span class="muted"><b>Figure 1. Robust cleavage of kinase genes with TrueGuide Synthetic gRNAs in Cas9-expressing ME-180 cells.</b> Average cleavage efficiency was >60%.</span></small></div></div><div class="clearfix"></div></div><div class="heading section"><h3> Data: TrueGuide Synthetic gRNAs help maintain cell viability </h3></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_8845"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.320.low.png/1725389960808.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.320.medium.png/1725389960808.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.480.medium.png/1725389960808.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.620.high.png/1725389960808.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.full.high.png/1725389960808.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.full.high.png/1725389960808.png,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.320.high.png/1725389960808.png,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.480.high.png/1725389960808.png,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.320.medium.png/1725389960808.png,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.620.high.png/1725389960808.png,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.320.low.png/1725389960808.png,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_8845/image.img.480.medium.png/1725389960808.png" alt='Two bar graphs showing high viability of cells transfected with TrueGuide Synthetic gRNAs' title='Two bar graphs showing cell viability when using TrueGuide Synthetic gRNAs'></picture></div><style> #cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_8845 { max-width: 100%; } #cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_8845 img { width: 100%; max-width: 1100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><small><span class="muted"><b>Figure 2. TrueGuide Synthetic gRNAs delivered with optimized protocols cause minimal cell toxicity.</b> Lipofectamine CRISPRMAX was used to complex and deliver <a adhocenable="false" href="/order/catalog/product/A36499">TrueCut Cas9 Protein v2</a> and target-specific TrueGuide Synthetic gRNAs into two cancer cell lines using our <a adhocenable="false" href="/sg/en/home/life-science/genome-editing/crispr-transfection.html">recommended transfection protocols</a>. Of the three loci, two (PRKCG-T1 and CMPK1-T1) were known to be challenging to edit. Cell viability was measured using <a adhocenable="false" href="/sg/en/home/life-science/cell-analysis/fluorescence-microplate-assays/microplate-assays-cell-viability/prestoblue-cell-viability-reagent.html">PrestoBlue.</a> Data showed transfection caused minimal toxicity, with overall cell viability maintained at close to 100%. </span></small></div></div><div class="clearfix"></div></div><div class="heading section"><h3 id="faq"> TrueGuide Synthetic Guide RNAs—Frequently asked questions (FAQs) </h3></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#gRNA"><span class="caret"></span> What is gRNA? </a></div><div class="accordion-body collapse " id="gRNA"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>gRNA, or guide RNA, is part of the CRISPR-Cas9 system. The gRNA molecule has two components: a target-specific CRISPR RNA (crRNA) and an auxiliary trans-activating crRNA (tracrRNA). The gRNA guides the Cas9 protein to a specific genomic locus via base pairing between the 20-mer crRNA sequence and the target genomic sequence. </p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#sgRNA"><span class="caret"></span> What is sgRNA? Do you offer a two-part synthetic gRNA? </a></div><div class="accordion-body collapse " id="sgRNA"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>sgRNA stands for single guide RNA, as opposed to the conventional, native <i>S. pyogenes</i> CRISPR-Cas9 system that uses a two-part guide in which CRISPR RNA (crRNA) is hybridized with a tracrRNA. An sgRNA combines both RNAs into a single, long RNA. sgRNAs are typically 100 nt in length and can be expressed from a vector or chemically synthesized.</p><p>Because the modified sgRNA demonstrates superior efficiency across more cell types than the two-part system, the TrueGuide crRNA:tracrRNA two-part guide RNA product format has been discontinued. This has allowed us to simplify our synthetic gRNA offering and focus on the modified sgRNA.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#sgRNAformats"><span class="caret"></span> What gRNA formats are available? </a></div><div class="accordion-body collapse " id="sgRNAformats"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>CRISPR gRNAs are available from Thermo Fisher Scientific as chemically synthesized RNA oligos, packaged lentiviral particles, or generated as <i>in vitro</i> transcribed (IVT) gRNAs using the <a href="#geneart">GeneArt Precision gRNA Synthesis Kit.</a></p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#gRNAschemicallymodified"><span class="caret"></span> Are TrueGuide Synthetic gRNAs chemically modified? Can I get an unmodified version? </a></div><div class="accordion-body collapse " id="gRNAschemicallymodified"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>Chemical modifications include 2’ O-Methyl analogs and phosphorothioate internucleotide linkages at the 5′ and 3′ ends of the molecule. These modifications enhance editing efficiency by increasing binding to the target site and inhibiting nuclease degradation, respectively.</p><p>You can purchase an sgRNA without any chemical modifications by using the <a href="/order/custom-oligo/crispr">custom gRNA order tool</a>.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#trueGuidesyntheticgRNA"><span class="caret"></span> Why should I use TrueGuide Synthetic gRNA instead of plasmid-expressed gRNA? </a></div><div class="accordion-body collapse " id="trueGuidesyntheticgRNA"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>TrueGuide Synthetic gRNA, when transfected with <a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-proteins.html">TrueCut Cas9 Protein,</a> offers maximum editing efficiency across broad cell types while minimizing cell toxicity and innate immune responses. Unlike a plasmid-based system, the RNP system is transient with faster turnover, which minimizes random integrations and off-target effects.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#qualitytested"><span class="caret"></span> How is TrueGuide Synthetic gRNA quality-tested? </a></div><div class="accordion-body collapse " id="qualitytested"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>TrueGuide Synthetic gRNA sequences are analyzed by mass spectrometry to verify sequence integrity.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#measuring_"><span class="caret"></span> What is the best method for measuring cleavage efficiency? </a></div><div class="accordion-body collapse " id="measuring_"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>To estimate CRISPR/Cas9 mediated editing efficiency in a pooled cell population, you can use the <a href="/order/catalog/product/A24372">GeneArt Genomic Cleavage Detection Kit,</a> next-generation sequencing (NGS), or Sanger sequencing. Either sequencing method—NGS of the amplicons from edited populations, or Sanger sequencing of amplicons cloned into plasmids—gives a more accurate estimate of percent editing efficiency and indel types. See the <a href="/sg/en/home/life-science/genome-editing/crispr-validation.html">TALEN and CRISPR Validation Tools page</a> for a more extensive discussion of these methods, and the TrueGuide Synthetic gRNA User Guide for protocol guidance.</p></div></div></div></div></div></div></div><div class="heading section"><h2 id="lentiarray"><br>LentiArray lentiviral gRNA for screening and editing hard-to-transfect cell lines </h2></div><div class="text parbase section"><p>Invitrogen LentiArray CRISPR gRNA are pre-designed, pre-packaged gRNA lentiviruses designed for efficient gene knockout. LentiArray CRISPR gRNA designs have been created with a proprietary gRNA design algorithm and are optimized for maximum knockout efficiency without compromising specificity. </p><p>LentiArray CRISPR gRNAs are available to support pre-screen assay development and post-screen hit validation when performing high-throughput screening experiments with the <a href="/sg/en/home/life-science/genome-editing/crispr-libraries.html">LentiArray CRISPR libraries</a>. LentiArray CRISPR gRNA should be used with LentiArray <a href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-lentivirus.html">Cas9 Lentivirus</a> to perform genome editing experiments in hard-to-transfect cell lines and primary cells.</p></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/life-science/genome-editing/crispr-libraries/lentiarray-crispr-libraries/lentiarray-grnas.html">Find LentiArray CRISPR gRNA using our selection tool</a></p></div><div class="heading section"><h3> Data: Successful knockout of EGFP with LentiArray gRNA </h3></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_e320"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.320.high.jpg/1725389961046.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.480.high.jpg/1725389961046.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.full.high.jpg/1725389961046.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.320.high.jpg/1725389961046.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.480.high.jpg/1725389961046.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.320.medium.jpg/1725389961046.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.620.high.jpg/1725389961046.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.320.low.jpg/1725389961046.jpg,https://www.thermofisher.com/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/textimage_e320/image.img.480.medium.jpg/1725389961046.jpg" alt='Knockout of EGFR expression with LentiArray gRNA shown via analysis of immunofluorescence is stained cells as well as western blot analysis' title='Stained cells and western blot images showing EGFP knockout'></picture></div><style> #cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_e320 { max-width: 100%; } #cq-textimage-jsp-76440bcf-4db7-4401-b9a8-fa994fd97500MainParsystextimage_e320 img { width: 100%; max-width: 613px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 3. CRISPR-Cas9-mediated knockout of EGFP with LentiArray gRNA.</b><b>(A)</b> A431 cells were subjected to EGFR knockout (right column) using LentiArray Cas9 Lentivirus and LentiArray CRISPR gRNA and compared with untreated cells (wild type, left column) and cells treated with Cas9 but no gRNA (Cas9 control, middle column). Cells were stained for EGFR expression (green), nuclei (blue), and cytoskeletal F-actin (red), and analyzed for immunofluorescence. Loss of EGFR expression was observed only in EGFR knockout cells (c, f). <b>(B)</b> Reduction of EGFR signal was also observed via western blot for target protein in EGFR knockout cells vs controls.</span></small></p></div></div><div class="clearfix"></div></div><div class="divider section"><hr /></div><div class="text parbase section"><ul class="the-icons"><li> <a class="cta-link" href="/sg/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries/lentiarray-crispr-libraries/lentiarray-grnas.html">Order now with our LentiArray CRISPR gRNA selection tool</a></li><li><span class="icon-lt-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016089_LentiArray_CRISPR_gRNA_UG.pdf">Download the LentiArray CRISPR gRNA Lentivirus user guide</a></li></ul></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="geneart"> IVT gRNA for rapid generation of custom designs </h2></div><div class="text parbase section"><p>The GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA). </p><p>With this kit, two short single-stranded oligos that code for the target sequence serve as the starting material, and the gRNA template is assembled from these oligos with a T7 promoter in a short “one-pot” PCR reaction. Following this, the assembled product is then used as template in an <i>in vitro</i> transcription (IVT) reaction. A subsequent rapid purification step produces high-yield (>10 µg), high-concentration (>200 ng/µL), transfection-ready gRNA in as little as four hours.</p><p>Combining IVT gRNA with our TrueCut Cas9 Protein v2 has been tested in a variety of suspension and adherent cell lines with >70% cleavage efficiencies and no indications of toxicity. </p><p>The resulting gRNA can also be co-transfected with our ready-to-transfect <a adhocenable="false" href="/sg/en/home/life-science/genome-editing/cas9-nucleases/cas9-mrna.html">CRISPR Nuclease mRNA</a>. Both protein and mRNA Cas9 formats require no plasmid manipulation and are compatible with high-throughput and multiplex genome-wide cell engineering approaches.</p></div><div class="text parbase section"><ul class="the-icons"><li><span class="icon-lt-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/geneart_precision_gRNA_synthesis_kit_man.pdf">Download the GeneArt Precision gRNA Synthesis Kit user guide</a></li><li> <a class="cta-link" href="mailto:GEMservices@thermofisher.com">Contact us to order custom IVT gRNAs</a></li></ul></div><div class="heading section"><h3 id="order"> Order the IVT gRNA synthesis kit </h3></div><div class="productlist parbase section"><div id="skulist" style="display:none">A29377</div><div id='ltajax-7724894667270003479'></div><script type="text/javascript">(function() {$('#ltajax-7724894667270003479').load('/sg/en/home/life-science/genome-editing/crispr-grna/jcr:content/MainParsys/productlist_e75d.ajax.html?skulist=');})(); </script></div></div><div><div class="MainInheritParsys iparsys parsys"><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Gene editing resources and support </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><div class="h3"> Resources </div></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><ul class="the-icons"><li><a href="/sg/en/home/life-science/genome-editing/genome-editing-learning-center.html">Genome Editing Learning Center</a></li><li><a href="/sg/en/home/life-science/genome-editing/genome-editing-learning-center/genome-editing-resource-library.html">Genome Editing Resource Library</a></li><li><a href="/sg/en/home/global/forms/genome-editing-resource-guide-form.html">CRISPR Genome Editing Resource Guide</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/complete-cell-engineering-solutions-brochure.pdf" target="_blank">CRISPR Genome Editing Brochure</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><div class="h3"> Support </div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><ul class="the-icons"><li style="text-align: justify;"><a href="/sg/en/home/technical-resources/technical-reference-library/genome-editing-support-center.html">Genome Editing Support Center</a></li><li><a href="/sg/en/home/global/forms/gene-editing-crispr-quote-request.html">Request Genome Editing Support</a></li><li><a href="/sg/en/home/life-science/genome-editing/genome-engineering-services/cell-line-engineering-services.html">Cell Line Engineering Services</a></li><li><a href="mailto:techsupport@thermofisher.com">Contact Technical Support</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="LTRawHTML section"><style> /* Table Styles Start */ table.rte-table-1 tr th { background-color: #fbfbfb; background-image: -moz-linear-gradient(top,#fff,#e8e8e8); background-image: -webkit-gradient(linear,0 0,0 100%,from(#fff),to(#e8e8e8)); background-image: -webkit-linear-gradient(top,#fff,#e8e8e8); background-image: -o-linear-gradient(top,#fff,#e8e8e8); background-image: none; background-repeat: repeat-x; vertical-align: bottom; } table.rte-table-1 tr td, table.rte-table tr th { border: none; border-bottom: 1px solid #d8d8d8; padding: 8px; text-align: left; } /* Table Styles End */ </style></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="text_72be text parbase"><p>For Research Use Only. 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