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has-global-padding is-layout-constrained wp-block-group-is-layout-constrained" style="margin-top:0;margin-bottom:0"> <div class="wp-block-group alignwide has-small-font-size is-layout-flow wp-block-group-is-layout-flow"> <div class="wp-block-group has-small-font-size is-layout-flow wp-container-core-group-is-layout-9 wp-block-group-is-layout-flow" style="margin-top:0;margin-bottom:0;padding-top:0px;padding-right:0;padding-bottom:0px;padding-left:0"></div> </div> </div> </div></header> <main class="wp-block-group is-layout-flow wp-block-group-is-layout-flow"><h1 style="margin-bottom:var(--wp--preset--spacing--40);" class="has-text-align-center alignwide is-style-carre is-style-ligne wp-block-post-title has-var-wp-custom-typography-font-size-huge-clamp-2-25-rem-4-vw-2-75-rem-font-size">Thèse</h1> <div class="wp-block-group alignwide has-global-padding is-layout-constrained wp-block-group-is-layout-constrained" id="theses"> <div class="alignwide"> <div id="These_64034" class="Bloc_These" itemscope itemtype="https://schema.org/Thesis"> <div class="These_Details"> <h3 class="These_Titre" itemprop="name">Diagnostic précoce du sepsis à l’aide d’une biopuce à base de capteurs GMR</h3> <div class="These_Domaine"> <label>Domaine de recherche</label> <div class="These_Domaine_Principal" itemprop="inSupportOf">Technologies pour la santé et l’environnement, dispositifs médicaux</div> <div class="These_Domaine_Secondaire"></div> </div> <div class="These_Resume"> <label>Sujet</label> <div itemprop="text">Le sepsis, réponse immunitaire extrême et dérégulée face à une infection qui se propage alors dans le sang, peut entraîner un dysfonctionnement d’organes pouvant conduire à la mort du patient (11 millions de morts dans le monde chaque année). La biopuce brevetée à base de capteurs GMR (Giant MagnetoResistance) que nous avons développée présente un réel potentiel pour une détection précoce des agents pathogènes impliqués dans le sepsis ou des biomarqueurs de la maladie, présents en très faible quantité dans le sang, sans étape de culture. L’approche innovante que nous proposons est transversale car basée sur l’utilisation de nanoparticules magnétiques (NPM), fonctionnalisées par des anticorps monoclonaux produits au laboratoire LERI, dirigés contre les objets biologiques cibles (cellules bactéries levures etc..) qui sont détectées un à un dynamiquement et de manière simultanée par les capteurs GMR disposés de part et d’autre d’un canal microfluidique dans lequel ils circulent. La preuve de concept de cette biopuce a été obtenue sur un modèle de cellules myélomateuses murines. Nous avons pu atteindre une sensibilité et une spécificité avec ce modèle qui rend notre technique très compétitive par rapport aux tests Point-of-Care existants. Il est cependant nécessaire de valider ces résultats sur des agents pathogènes. <br> <br> Au cours de la thèse, deux objectifs seront définis. Dans la continuité de la thèse actuelle, Le premier objectif de l’étudiant au LNO sera d’adapter la biopuce (capteurs, microfluidique et traitement des signaux) afin qu’elle soit sensible et rapide pour la détection de bactéries et de levures impliqués dans le sepsis dans des échantillons sanguins. Au LERI, il devra optimiser le marquage magnétique des bactéries et des levures dans cette matrice clinique au moyen de NPM commerciales fonctionnalisées par un ou plusieurs anticorps dirigés contre la cible. Cette étape de la thèse se déroulera en étroite collaboration avec le Service de Bactériologie et Hygiène de l’hôpital Béclère (également membre de l’IHU) qui préconisera et fournira des souches de bactéries et levures pertinentes à détecter, ainsi que des échantillons cliniques. Une des biopuces GMR sera installée à l’Hôpital Béclère pour des mesures en conditions réelles. Le second objectif consistera à essayer de quantifier la diminution de l’expression monocytaire des molécules mHLA-DR qui est un indicateur de l’état d’immunosuppression du sepsis associé à un risque infectieux et une mortalité majorés.</div> </div> <div class="These_En_savoir_plus"> <div class="wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex"> <div class="wp-block-button is-style-button-liste"><a class="wp-block-button__link wp-element-button" href="https://instn.cea.fr/these/diagnostic-precoce-du-sepsis-a-laide-dune-biopuce-a-base-de-capteurs-gmr/" target="_blank">En savoir plus</a></div> </div> </div> </div> <div class="These_Informations"> <label>Informations pratiques</label> <div class="These_Code"> <label>Référence</label> <div>SL-DRF-25-0339</div> </div> <div class="These_Formation"> <label>Formation recommandée</label> <div itemprop="educationalLevel">Physicien ou biologiste avec des compétences transverses</div> </div> <div class="These_Date"> <label>Date souhaitée de début de thèse</label> <div>1 octobre 2025</div> </div> <div class="These_Lieu"> <label>Lieu</label> <div itemprop="contentLocation"> <div class="These_Lieu_Etablissement">Paris-Saclay</div> <div class="These_Lieu_Ecole">Physique en Île-de-France (EDPIF)</div> <div class="These_Lieu_Centre">Saclay</div> </div> </div> <div class="These_Laboratoire"> <label>Laboratoire</label> <div itemprop="sourceOrganization"> <div class="These_CEA">CEA</div> <div class="These_Labo_Pole">Direction de la Recherche Fondamentale</div> <div class="These_Labo_Departement">Institut rayonnement et matière de Saclay</div> <div class="These_Labo_Service">Service de Physique de l’Etat Condensé</div> <div class="These_Labo_Laboratoire">Laboratoire Nano-Magnétisme et Oxydes</div> </div> </div> <div class="These_Contacts"> <label>Contact</label> <div class="These_Contact"> <label>Encadrant</label> <div class="These_Contact_Prenom_NOM" itemprop="author">Guenaelle JASMIN-LEBRAS</div> <div class="These_Contact_Organisme">CEA</div> <div class="These_Contact_Laboratoire"><a href="https://iramis.cea.fr/spec/lno/pisp/guenaelle-jasmin-lebras/" target="_blank">DRF/IRAMIS/SPEC/LNO</a></div> <div class="These_Contact_Telephone">Tél. : <a href="tel:0169086535">01 69 08 65 35</a> </div> <div class="These_Contact_Email">Email : <a href="#" data-cea-protection-click="iwgpcgnng0lcuokp/ngdtcuBegc0ht"><span data-cea-protection="iwgpcgnng0lcuokp/ngdtcuBegc0ht"></span></a> </div> </div> <div class="These_Contact"> <label>Directeur de thèse</label> <div class="These_Contact_Prenom_NOM" itemprop="author">Guénaëlle JASMIN</div> <div class="These_Contact_Organisme">CEA</div> <div class="These_Contact_Laboratoire"><a href="https://iramis.cea.fr/spec/lno/pisp/guenaelle-jasmin-lebras/" target="_blank">DRF/IRAMIS/SPEC/LNO</a></div> <div class="These_Contact_Telephone">Tél. : <a href="tel:0169086535">01 69 08 65 35</a> </div> <div class="These_Contact_Email">Email : <a href="#" 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