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href="#Raman_shift"> <div class="vector-toc-text"> 3 Raman shift </div> </a> <ul id="toc-Raman_shift-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Instrumentation" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Instrumentation"> <div class="vector-toc-text"> 4 Instrumentation </div> </a> <button aria-controls="toc-Instrumentation-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> Toggle Instrumentation subsection </button> <ul id="toc-Instrumentation-sublist" class="vector-toc-list"> <li id="toc-Lasers" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Lasers"> <div class="vector-toc-text"> 4.1 Lasers </div> </a> <ul id="toc-Lasers-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Detectors" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Detectors"> <div class="vector-toc-text"> 4.2 Detectors </div> </a> <ul id="toc-Detectors-sublist" class="vector-toc-list"> <li id="toc-Detectors_for_dispersive_Raman" class="vector-toc-list-item vector-toc-level-3"> <a class="vector-toc-link" href="#Detectors_for_dispersive_Raman"> <div class="vector-toc-text"> 4.2.1 Detectors for dispersive Raman </div> </a> <ul id="toc-Detectors_for_dispersive_Raman-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Detectors_for_FT–Raman" class="vector-toc-list-item vector-toc-level-3"> <a class="vector-toc-link" href="#Detectors_for_FT–Raman"> <div class="vector-toc-text"> 4.2.2 Detectors for FT–Raman </div> </a> <ul id="toc-Detectors_for_FT–Raman-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-Filters" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Filters"> <div class="vector-toc-text"> 4.3 Filters </div> </a> <ul id="toc-Filters-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-Applications" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Applications"> <div class="vector-toc-text"> 5 Applications </div> </a> <button aria-controls="toc-Applications-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> Toggle Applications subsection </button> <ul id="toc-Applications-sublist" class="vector-toc-list"> <li id="toc-Art_and_cultural_heritage" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Art_and_cultural_heritage"> <div class="vector-toc-text"> 5.1 Art and cultural heritage </div> </a> <ul id="toc-Art_and_cultural_heritage-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-Microspectroscopy" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Microspectroscopy"> <div class="vector-toc-text"> 6 Microspectroscopy </div> </a> <ul id="toc-Microspectroscopy-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Polarization_dependence_of_Raman_scattering" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Polarization_dependence_of_Raman_scattering"> <div class="vector-toc-text"> 7 Polarization dependence of Raman scattering </div> </a> <button aria-controls="toc-Polarization_dependence_of_Raman_scattering-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> Toggle Polarization dependence of Raman scattering subsection </button> <ul id="toc-Polarization_dependence_of_Raman_scattering-sublist" class="vector-toc-list"> <li id="toc-Characterization_of_the_symmetry_of_a_vibrational_mode" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Characterization_of_the_symmetry_of_a_vibrational_mode"> <div class="vector-toc-text"> 7.1 Characterization of the symmetry of a vibrational mode </div> </a> <ul id="toc-Characterization_of_the_symmetry_of_a_vibrational_mode-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Raman_Excitation_Profile_Analysis" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Raman_Excitation_Profile_Analysis"> <div class="vector-toc-text"> 7.2 Raman Excitation Profile Analysis </div> </a> <ul id="toc-Raman_Excitation_Profile_Analysis-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-Variants" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Variants"> <div class="vector-toc-text"> 8 Variants </div> </a> <button aria-controls="toc-Variants-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> Toggle Variants subsection </button> <ul id="toc-Variants-sublist" class="vector-toc-list"> <li id="toc-Spontaneous_(or_far-field)_Raman_spectroscopy" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Spontaneous_(or_far-field)_Raman_spectroscopy"> <div class="vector-toc-text"> 8.1 Spontaneous (or far-field) Raman spectroscopy </div> </a> <ul id="toc-Spontaneous_(or_far-field)_Raman_spectroscopy-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Enhanced_(or_near-field)_Raman_spectroscopy" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Enhanced_(or_near-field)_Raman_spectroscopy"> <div class="vector-toc-text"> 8.2 Enhanced (or near-field) Raman spectroscopy </div> </a> <ul id="toc-Enhanced_(or_near-field)_Raman_spectroscopy-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Non-linear_Raman_spectroscopy" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Non-linear_Raman_spectroscopy"> <div class="vector-toc-text"> 8.3 Non-linear Raman spectroscopy </div> </a> <ul id="toc-Non-linear_Raman_spectroscopy-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Morphologically-Directed_Raman_spectroscopy" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Morphologically-Directed_Raman_spectroscopy"> <div class="vector-toc-text"> 8.4 Morphologically-Directed Raman spectroscopy </div> </a> <ul id="toc-Morphologically-Directed_Raman_spectroscopy-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-References" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#References"> <div class="vector-toc-text"> 9 References </div> </a> <ul id="toc-References-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Further_reading" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Further_reading"> <div class="vector-toc-text"> 10 Further reading </div> </a> <ul id="toc-Further_reading-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-External_links" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#External_links"> <div class="vector-toc-text"> 11 External links </div> </a> <ul id="toc-External_links-sublist" class="vector-toc-list"> </ul> </li> </ul> </div> </div> </nav> </div> </div> <div class="mw-content-container"> <main id="content" class="mw-body"> <header class="mw-body-header vector-page-titlebar"> <nav aria-label="Contents" class="vector-toc-landmark"> <div id="vector-page-titlebar-toc" class="vector-dropdown vector-page-titlebar-toc vector-button-flush-left" > <input type="checkbox" id="vector-page-titlebar-toc-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-page-titlebar-toc" class="vector-dropdown-checkbox " aria-label="Toggle the table of contents" > <label id="vector-page-titlebar-toc-label" for="vector-page-titlebar-toc-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button 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Available in 34 languages" > <label id="p-lang-btn-label" for="p-lang-btn-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--action-progressive mw-portlet-lang-heading-34" aria-hidden="true" > 34 languages </label> <div class="vector-dropdown-content"> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li class="interlanguage-link interwiki-ar mw-list-item"><a href="https://ar.wikipedia.org/wiki/%D9%85%D8%B7%D9%8A%D8%A7%D9%81%D9%8A%D8%A9_%D8%B1%D8%A7%D9%85%D8%A7%D9%86" title="مطيافية رامان – Arabic" lang="ar" hreflang="ar" data-title="مطيافية رامان" data-language-autonym="العربية" data-language-local-name="Arabic" class="interlanguage-link-target">العربية</a></li><li class="interlanguage-link interwiki-be-x-old mw-list-item"><a href="https://be-tarask.wikipedia.org/wiki/%D0%A0%D0%B0%D0%BC%D0%B0%D0%BD%D0%B0%D0%B2%D0%B0_%D1%81%D0%BF%D1%8D%D0%BA%D1%82%D1%80%D0%B0%D1%81%D0%BA%D0%BE%D0%BF%D1%96%D1%8F" title="Раманава спэктраскопія – Belarusian (Taraškievica orthography)" lang="be-tarask" hreflang="be-tarask" data-title="Раманава спэктраскопія" data-language-autonym="Беларуская (тарашкевіца)" data-language-local-name="Belarusian (Taraškievica orthography)" class="interlanguage-link-target">Беларуская (тарашкевіца)</a></li><li class="interlanguage-link interwiki-ca mw-list-item"><a href="https://ca.wikipedia.org/wiki/Espectrosc%C3%B2pia_Raman" title="Espectroscòpia Raman – Catalan" lang="ca" hreflang="ca" data-title="Espectroscòpia Raman" data-language-autonym="Català" data-language-local-name="Catalan" class="interlanguage-link-target">Català</a></li><li class="interlanguage-link interwiki-cs mw-list-item"><a href="https://cs.wikipedia.org/wiki/Ramanova_spektroskopie" title="Ramanova spektroskopie – Czech" lang="cs" hreflang="cs" data-title="Ramanova spektroskopie" data-language-autonym="Čeština" data-language-local-name="Czech" class="interlanguage-link-target">Čeština</a></li><li class="interlanguage-link interwiki-da mw-list-item"><a href="https://da.wikipedia.org/wiki/Ramanspektroskopi" title="Ramanspektroskopi – Danish" lang="da" hreflang="da" data-title="Ramanspektroskopi" data-language-autonym="Dansk" data-language-local-name="Danish" class="interlanguage-link-target">Dansk</a></li><li class="interlanguage-link interwiki-de mw-list-item"><a href="https://de.wikipedia.org/wiki/Raman-Spektroskopie" title="Raman-Spektroskopie – German" lang="de" hreflang="de" data-title="Raman-Spektroskopie" data-language-autonym="Deutsch" data-language-local-name="German" class="interlanguage-link-target">Deutsch</a></li><li class="interlanguage-link interwiki-el mw-list-item"><a href="https://el.wikipedia.org/wiki/%CE%A6%CE%B1%CF%83%CE%BC%CE%B1%CF%84%CE%BF%CF%83%CE%BA%CE%BF%CF%80%CE%AF%CE%B1_%CE%A1%CE%AC%CE%BC%CE%B1%CE%BD" title="Φασματοσκοπία Ράμαν – Greek" lang="el" hreflang="el" data-title="Φασματοσκοπία Ράμαν" data-language-autonym="Ελληνικά" data-language-local-name="Greek" class="interlanguage-link-target">Ελληνικά</a></li><li class="interlanguage-link interwiki-es mw-list-item"><a href="https://es.wikipedia.org/wiki/Espectroscopia_Raman" title="Espectroscopia Raman – Spanish" lang="es" hreflang="es" data-title="Espectroscopia Raman" data-language-autonym="Español" data-language-local-name="Spanish" class="interlanguage-link-target">Español</a></li><li class="interlanguage-link interwiki-eu mw-list-item"><a href="https://eu.wikipedia.org/wiki/Raman_espektroskopia" title="Raman espektroskopia – Basque" lang="eu" hreflang="eu" data-title="Raman espektroskopia" data-language-autonym="Euskara" data-language-local-name="Basque" class="interlanguage-link-target">Euskara</a></li><li class="interlanguage-link interwiki-fa mw-list-item"><a href="https://fa.wikipedia.org/wiki/%D8%B7%DB%8C%D9%81%E2%80%8C%D8%B4%D9%86%D8%A7%D8%B3%DB%8C_%D8%B1%D8%A7%D9%85%D8%A7%D9%86" title="طیف‌شناسی رامان – Persian" lang="fa" hreflang="fa" data-title="طیف‌شناسی رامان" data-language-autonym="فارسی" data-language-local-name="Persian" class="interlanguage-link-target">فارسی</a></li><li class="interlanguage-link interwiki-fr mw-list-item"><a href="https://fr.wikipedia.org/wiki/Spectroscopie_Raman" title="Spectroscopie Raman – French" lang="fr" hreflang="fr" data-title="Spectroscopie Raman" data-language-autonym="Français" data-language-local-name="French" class="interlanguage-link-target">Français</a></li><li class="interlanguage-link interwiki-ko mw-list-item"><a href="https://ko.wikipedia.org/wiki/%EB%9D%BC%EB%A7%8C_%EB%B6%84%EA%B4%91%EB%B2%95" title="라만 분광법 – Korean" lang="ko" hreflang="ko" data-title="라만 분광법" data-language-autonym="한국어" data-language-local-name="Korean" class="interlanguage-link-target">한국어</a></li><li class="interlanguage-link interwiki-hi mw-list-item"><a href="https://hi.wikipedia.org/wiki/%E0%A4%B0%E0%A4%AE%E0%A4%A8_%E0%A4%B8%E0%A5%8D%E0%A4%AA%E0%A5%87%E0%A4%95%E0%A5%8D%E0%A4%9F%E0%A5%8D%E0%A4%B0%E0%A4%AE%E0%A4%BF%E0%A4%95%E0%A5%80" title="रमन स्पेक्ट्रमिकी – Hindi" lang="hi" hreflang="hi" data-title="रमन स्पेक्ट्रमिकी" data-language-autonym="हिन्दी" data-language-local-name="Hindi" class="interlanguage-link-target">हिन्दी</a></li><li class="interlanguage-link interwiki-hr mw-list-item"><a href="https://hr.wikipedia.org/wiki/Ramanova_spektroskopija" title="Ramanova spektroskopija – Croatian" lang="hr" hreflang="hr" data-title="Ramanova spektroskopija" data-language-autonym="Hrvatski" data-language-local-name="Croatian" class="interlanguage-link-target">Hrvatski</a></li><li class="interlanguage-link interwiki-id mw-list-item"><a href="https://id.wikipedia.org/wiki/Spektroskopi_Raman" title="Spektroskopi Raman – Indonesian" lang="id" hreflang="id" data-title="Spektroskopi Raman" data-language-autonym="Bahasa Indonesia" data-language-local-name="Indonesian" class="interlanguage-link-target">Bahasa Indonesia</a></li><li class="interlanguage-link interwiki-it mw-list-item"><a href="https://it.wikipedia.org/wiki/Spettroscopia_Raman" title="Spettroscopia Raman – Italian" lang="it" hreflang="it" data-title="Spettroscopia Raman" data-language-autonym="Italiano" data-language-local-name="Italian" class="interlanguage-link-target">Italiano</a></li><li class="interlanguage-link interwiki-kn mw-list-item"><a href="https://kn.wikipedia.org/wiki/%E0%B2%B0%E0%B2%BE%E0%B2%AE%E0%B2%A8%E0%B3%8D_%E0%B2%B8%E0%B3%8D%E0%B2%AA%E0%B3%86%E0%B2%95%E0%B3%8D%E0%B2%9F%E0%B3%8D%E0%B2%B0%E0%B3%8B%E0%B2%B8%E0%B3%8D%E0%B2%95%E0%B3%8A%E0%B2%AA%E0%B2%BF" title="ರಾಮನ್ ಸ್ಪೆಕ್ಟ್ರೋಸ್ಕೊಪಿ – Kannada" lang="kn" hreflang="kn" data-title="ರಾಮನ್ ಸ್ಪೆಕ್ಟ್ರೋಸ್ಕೊಪಿ" data-language-autonym="ಕನ್ನಡ" data-language-local-name="Kannada" class="interlanguage-link-target">ಕನ್ನಡ</a></li><li class="interlanguage-link interwiki-lv mw-list-item"><a href="https://lv.wikipedia.org/wiki/Ramana_spektroskopija" title="Ramana spektroskopija – Latvian" lang="lv" hreflang="lv" data-title="Ramana spektroskopija" data-language-autonym="Latviešu" data-language-local-name="Latvian" class="interlanguage-link-target">Latviešu</a></li><li class="interlanguage-link interwiki-lt mw-list-item"><a href="https://lt.wikipedia.org/wiki/Ramano_spektroskopija" title="Ramano spektroskopija – Lithuanian" lang="lt" hreflang="lt" data-title="Ramano spektroskopija" data-language-autonym="Lietuvių" data-language-local-name="Lithuanian" class="interlanguage-link-target">Lietuvių</a></li><li class="interlanguage-link interwiki-nl mw-list-item"><a href="https://nl.wikipedia.org/wiki/Ramanspectroscopie" title="Ramanspectroscopie – Dutch" lang="nl" hreflang="nl" data-title="Ramanspectroscopie" data-language-autonym="Nederlands" data-language-local-name="Dutch" class="interlanguage-link-target">Nederlands</a></li><li class="interlanguage-link interwiki-ja mw-list-item"><a href="https://ja.wikipedia.org/wiki/%E3%83%A9%E3%83%9E%E3%83%B3%E5%88%86%E5%85%89%E6%B3%95" title="ラマン分光法 – Japanese" lang="ja" hreflang="ja" data-title="ラマン分光法" data-language-autonym="日本語" data-language-local-name="Japanese" class="interlanguage-link-target">日本語</a></li><li class="interlanguage-link interwiki-pl mw-list-item"><a href="https://pl.wikipedia.org/wiki/Spektroskopia_Ramana" title="Spektroskopia Ramana – Polish" lang="pl" hreflang="pl" data-title="Spektroskopia Ramana" data-language-autonym="Polski" data-language-local-name="Polish" class="interlanguage-link-target">Polski</a></li><li class="interlanguage-link interwiki-pt mw-list-item"><a href="https://pt.wikipedia.org/wiki/Espectroscopia_Raman" title="Espectroscopia Raman – Portuguese" lang="pt" hreflang="pt" data-title="Espectroscopia Raman" data-language-autonym="Português" data-language-local-name="Portuguese" class="interlanguage-link-target">Português</a></li><li class="interlanguage-link interwiki-ro mw-list-item"><a href="https://ro.wikipedia.org/wiki/Spectroscopie_Raman" title="Spectroscopie Raman – Romanian" lang="ro" hreflang="ro" data-title="Spectroscopie Raman" data-language-autonym="Română" data-language-local-name="Romanian" class="interlanguage-link-target">Română</a></li><li class="interlanguage-link interwiki-ru mw-list-item"><a href="https://ru.wikipedia.org/wiki/%D0%A0%D0%B0%D0%BC%D0%B0%D0%BD%D0%BE%D0%B2%D1%81%D0%BA%D0%B0%D1%8F_%D1%81%D0%BF%D0%B5%D0%BA%D1%82%D1%80%D0%BE%D1%81%D0%BA%D0%BE%D0%BF%D0%B8%D1%8F" title="Рамановская спектроскопия – Russian" lang="ru" hreflang="ru" data-title="Рамановская спектроскопия" data-language-autonym="Русский" data-language-local-name="Russian" class="interlanguage-link-target">Русский</a></li><li class="interlanguage-link interwiki-sl mw-list-item"><a href="https://sl.wikipedia.org/wiki/Ramanska_spektroskopija" title="Ramanska spektroskopija – Slovenian" lang="sl" hreflang="sl" data-title="Ramanska spektroskopija" data-language-autonym="Slovenščina" data-language-local-name="Slovenian" class="interlanguage-link-target">Slovenščina</a></li><li class="interlanguage-link interwiki-sr mw-list-item"><a href="https://sr.wikipedia.org/wiki/Ramanova_spektroskopija" title="Ramanova spektroskopija – Serbian" lang="sr" hreflang="sr" data-title="Ramanova spektroskopija" data-language-autonym="Српски / srpski" data-language-local-name="Serbian" class="interlanguage-link-target">Српски / srpski</a></li><li class="interlanguage-link interwiki-sh mw-list-item"><a href="https://sh.wikipedia.org/wiki/Ramanova_spektroskopija" title="Ramanova spektroskopija – Serbo-Croatian" lang="sh" hreflang="sh" data-title="Ramanova spektroskopija" data-language-autonym="Srpskohrvatski / српскохрватски" data-language-local-name="Serbo-Croatian" class="interlanguage-link-target">Srpskohrvatski / српскохрватски</a></li><li class="interlanguage-link interwiki-tl mw-list-item"><a href="https://tl.wikipedia.org/wiki/Raman_spectroscopy" title="Raman spectroscopy – Tagalog" lang="tl" hreflang="tl" data-title="Raman spectroscopy" data-language-autonym="Tagalog" data-language-local-name="Tagalog" class="interlanguage-link-target">Tagalog</a></li><li class="interlanguage-link interwiki-te mw-list-item"><a href="https://te.wikipedia.org/wiki/%E0%B0%B0%E0%B0%BE%E0%B0%AE%E0%B0%A8%E0%B1%8D_%E0%B0%B8%E0%B1%8D%E0%B0%AA%E0%B1%86%E0%B0%95%E0%B1%8D%E0%B0%9F%E0%B1%8D%E0%B0%B0%E0%B1%8B%E0%B0%B8%E0%B1%8D%E0%B0%95%E0%B1%8B%E0%B0%AA%E0%B1%80" title="రామన్ స్పెక్ట్రోస్కోపీ – Telugu" lang="te" hreflang="te" data-title="రామన్ స్పెక్ట్రోస్కోపీ" data-language-autonym="తెలుగు" data-language-local-name="Telugu" class="interlanguage-link-target">తెలుగు</a></li><li class="interlanguage-link interwiki-tr mw-list-item"><a href="https://tr.wikipedia.org/wiki/Raman_spektroskopisi" title="Raman spektroskopisi – Turkish" lang="tr" hreflang="tr" data-title="Raman spektroskopisi" data-language-autonym="Türkçe" data-language-local-name="Turkish" class="interlanguage-link-target">Türkçe</a></li><li class="interlanguage-link interwiki-uk mw-list-item"><a href="https://uk.wikipedia.org/wiki/%D0%A0%D0%B0%D0%BC%D0%B0%D0%BD-%D1%81%D0%BF%D0%B5%D0%BA%D1%82%D1%80%D0%BE%D1%81%D0%BA%D0%BE%D0%BF%D1%96%D1%8F" title="Раман-спектроскопія – Ukrainian" lang="uk" hreflang="uk" data-title="Раман-спектроскопія" data-language-autonym="Українська" data-language-local-name="Ukrainian" class="interlanguage-link-target">Українська</a></li><li class="interlanguage-link interwiki-zh-yue mw-list-item"><a href="https://zh-yue.wikipedia.org/wiki/%E6%8B%89%E6%9B%BC%E5%85%89%E8%AD%9C%E5%AD%B8" title="拉曼光譜學 – Cantonese" lang="yue" hreflang="yue" data-title="拉曼光譜學" data-language-autonym="粵語" data-language-local-name="Cantonese" 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free encyclopedia</div> </div> <div id="contentSub"><div id="mw-content-subtitle"></div></div> <div id="mw-content-text" class="mw-body-content"><div class="mw-content-ltr mw-parser-output" lang="en" dir="ltr"><div class="shortdescription nomobile noexcerpt noprint searchaux" style="display:none">Spectroscopic technique</div> <figure typeof="mw:File/Thumb"><a href="/wiki/File:Raman_energy_levels.svg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/4/41/Raman_energy_levels.svg/250px-Raman_energy_levels.svg.png" decoding="async" width="250" height="191" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/4/41/Raman_energy_levels.svg/375px-Raman_energy_levels.svg.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/4/41/Raman_energy_levels.svg/500px-Raman_energy_levels.svg.png 2x" data-file-width="850" data-file-height="650" /></a><figcaption>Energy-level diagram showing the states involved in Raman spectra.</figcaption></figure> Raman spectroscopy (<a href="/wiki/Help:IPA/English" title="Help:IPA/English">/ˈrɑːmən/</a>) (named after physicist <a href="/wiki/C._V._Raman" title="C. V. Raman">C. V. Raman</a>) is a <a href="/wiki/Spectroscopy" title="Spectroscopy">spectroscopic</a> technique typically used to determine <a href="/wiki/Vibrational_mode" class="mw-redirect" title="Vibrational mode">vibrational modes</a> of <a href="/wiki/Molecule" title="Molecule">molecules</a>, although rotational and other low-frequency modes of systems may also be observed.<a href="#cite_note-Gardiner-1">[1]</a> Raman spectroscopy is commonly used in chemistry to provide a structural fingerprint by which molecules can be identified.<a href="#cite_note-2">[2]</a> Raman <a href="/wiki/Spectroscopy" title="Spectroscopy">spectroscopy</a> relies upon <a href="/wiki/Inelastic_scattering" title="Inelastic scattering">inelastic scattering</a> of photons, known as <a href="/wiki/Raman_scattering" title="Raman scattering">Raman scattering</a>. A source of <a href="/wiki/Monochromatic" class="mw-redirect" title="Monochromatic">monochromatic</a> light, usually from a <a href="/wiki/Laser" title="Laser">laser</a> in the <a href="/wiki/Visible_spectrum" title="Visible spectrum">visible</a>, near infrared, or <a href="/wiki/Ultraviolet" title="Ultraviolet">near ultraviolet</a> range is used, although <a href="/wiki/X-ray_Raman_scattering" title="X-ray Raman scattering">X-rays</a> can also be used. The laser light interacts with molecular vibrations, <a href="/wiki/Phonon" title="Phonon">phonons</a> or other excitations in the system, resulting in the energy of the laser photons being shifted up or down. The shift in energy gives information about the vibrational modes in the system. <a href="/wiki/Infrared_spectroscopy" title="Infrared spectroscopy">Infrared spectroscopy</a> typically yields similar yet complementary information. Typically, a sample is illuminated with a laser beam. Electromagnetic radiation from the illuminated spot is collected with a <a href="/wiki/Lens_(optics)" class="mw-redirect" title="Lens (optics)">lens</a> and sent through a <a href="/wiki/Monochromator" title="Monochromator">monochromator</a>.[<a href="/wiki/Wikipedia:Citation_needed" title="Wikipedia:Citation needed">citation needed</a>] Elastic scattered radiation at the wavelength corresponding to the laser line (<a href="/wiki/Rayleigh_scattering" title="Rayleigh scattering">Rayleigh scattering</a>) is filtered out by either a <a href="/wiki/Band-stop_filter" title="Band-stop filter">notch filter</a>, edge pass filter, or a band pass filter, while the rest of the collected light is dispersed onto a detector. Spontaneous <a href="/wiki/Raman_scattering" title="Raman scattering">Raman scattering</a> is typically very weak; as a result, for many years the main difficulty in collecting Raman spectra was separating the weak inelastically scattered light from the intense Rayleigh scattered laser light (referred to as "laser rejection"). Historically, Raman <a href="/wiki/Spectrometer" title="Spectrometer">spectrometers</a> used <a href="/wiki/Holographic_grating" title="Holographic grating">holographic gratings</a> and multiple dispersion stages to achieve a high degree of laser rejection. In the past, <a href="/wiki/Photomultiplier" title="Photomultiplier">photomultipliers</a> were the detectors of choice for dispersive Raman setups, which resulted in long acquisition times. However, modern instrumentation almost universally employs <a href="/wiki/Band-stop_filter" title="Band-stop filter">notch or edge filters</a> for laser rejection. Dispersive single-stage spectrographs (axial transmissive (AT) or <a href="/wiki/Monochromator#Czerny–Turner_monochromator" title="Monochromator">Czerny–Turner (CT) monochromators</a>) paired with <a href="/wiki/Charge-coupled_device" title="Charge-coupled device">CCD</a> detectors are most common although <a href="/wiki/Fourier_transform_spectroscopy" class="mw-redirect" title="Fourier transform spectroscopy">Fourier transform</a> (FT) spectrometers are also common for use with NIR lasers. The name "Raman spectroscopy" typically refers to vibrational Raman using laser wavelengths which are not absorbed by the sample. There are many other variations of Raman spectroscopy including <a href="/wiki/Surface_enhanced_Raman_spectroscopy" class="mw-redirect" title="Surface enhanced Raman spectroscopy">surface-enhanced Raman</a>, <a href="/wiki/Resonance_Raman_spectroscopy" title="Resonance Raman spectroscopy">resonance Raman</a>, <a href="/wiki/Tip-enhanced_Raman_spectroscopy" title="Tip-enhanced Raman spectroscopy">tip-enhanced Raman</a>, polarized Raman, <a href="/wiki/Raman_scattering#Stimulated_Raman_scattering_and_Raman_amplification" title="Raman scattering">stimulated Raman</a>, transmission Raman, spatially-offset Raman, and <a href="/wiki/Resonance_Raman_spectroscopy#Resonance_hyper_Raman_spectroscopy" title="Resonance Raman spectroscopy">hyper Raman</a>. <meta property="mw:PageProp/toc" /> <div class="mw-heading mw-heading2"><h2 id="History">History</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=1" title="Edit section: History">edit</a>]</div> Although the inelastic scattering of light was predicted by <a href="/wiki/Adolf_Smekal" title="Adolf Smekal">Adolf Smekal</a> in 1923,<a href="#cite_note-3">[3]</a> it was not observed in practice until 1928. The Raman effect was named after one of its discoverers, the Indian scientist <a href="/wiki/C._V._Raman" title="C. V. Raman">C. V. Raman</a>, who observed the effect in organic liquids in 1928 together with <a href="/wiki/K._S._Krishnan" title="K. S. Krishnan">K. S. Krishnan</a>, and independently by <a href="/wiki/Grigory_Landsberg" title="Grigory Landsberg">Grigory Landsberg</a> and <a href="/wiki/Leonid_Isaakovich_Mandelstam" class="mw-redirect" title="Leonid Isaakovich Mandelstam">Leonid Mandelstam</a> in inorganic crystals.<a href="#cite_note-Gardiner-1">[1]</a> Raman won the <a href="/wiki/Nobel_Prize_in_Physics" title="Nobel Prize in Physics">Nobel Prize in Physics</a> in 1930 for this discovery. The first observation of Raman spectra in gases was in 1929 by <a href="/wiki/Franco_Rasetti#Raman_spectroscopy_and_the_model_of_the_atomic_nucleus" title="Franco Rasetti">Franco Rasetti</a>.<a href="#cite_note-4">[4]</a><a href="#cite_note-5">[5]</a> Systematic pioneering theory of the Raman effect was developed by Czechoslovak physicist <a href="/wiki/George_Placzek" title="George Placzek">George Placzek</a> between 1930 and 1934.<a href="#cite_note-6">[6]</a> The mercury <a href="/wiki/Arc_lamp" title="Arc lamp">arc</a> became the principal light source, first with photographic detection and then with spectrophotometric detection. In the years following its discovery, Raman spectroscopy was used to provide the first catalog of molecular vibrational frequencies. Typically, the sample was held in a long tube and illuminated along its length with a beam of filtered <a href="/wiki/Monochromatic" class="mw-redirect" title="Monochromatic">monochromatic</a> light generated by a <a href="/wiki/Gas_discharge_lamp" class="mw-redirect" title="Gas discharge lamp">gas discharge lamp</a>. The photons that were scattered by the sample were collected through an <a href="/wiki/Optical_flat" title="Optical flat">optical flat</a> at the end of the tube. To maximize the sensitivity, the sample was highly concentrated (1 M or more) and relatively large volumes (5 mL or more) were used. <div class="mw-heading mw-heading2"><h2 id="Theory">Theory</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=2" title="Edit section: Theory">edit</a>]</div> <style data-mw-deduplicate="TemplateStyles:r1236090951">.mw-parser-output .hatnote{font-style:italic}.mw-parser-output div.hatnote{padding-left:1.6em;margin-bottom:0.5em}.mw-parser-output .hatnote i{font-style:normal}.mw-parser-output .hatnote+link+.hatnote{margin-top:-0.5em}@media print{body.ns-0 .mw-parser-output .hatnote{display:none!important}}</style><div role="note" class="hatnote navigation-not-searchable">Main article: <a href="/wiki/Raman_scattering" title="Raman scattering">Raman scattering</a></div> <style data-mw-deduplicate="TemplateStyles:r1251242444">.mw-parser-output .ambox{border:1px solid #a2a9b1;border-left:10px solid #36c;background-color:#fbfbfb;box-sizing:border-box}.mw-parser-output .ambox+link+.ambox,.mw-parser-output .ambox+link+style+.ambox,.mw-parser-output .ambox+link+link+.ambox,.mw-parser-output .ambox+.mw-empty-elt+link+.ambox,.mw-parser-output .ambox+.mw-empty-elt+link+style+.ambox,.mw-parser-output .ambox+.mw-empty-elt+link+link+.ambox{margin-top:-1px}html body.mediawiki .mw-parser-output .ambox.mbox-small-left{margin:4px 1em 4px 0;overflow:hidden;width:238px;border-collapse:collapse;font-size:88%;line-height:1.25em}.mw-parser-output .ambox-speedy{border-left:10px solid #b32424;background-color:#fee7e6}.mw-parser-output .ambox-delete{border-left:10px solid #b32424}.mw-parser-output .ambox-content{border-left:10px solid #f28500}.mw-parser-output .ambox-style{border-left:10px solid #fc3}.mw-parser-output .ambox-move{border-left:10px solid #9932cc}.mw-parser-output .ambox-protection{border-left:10px solid #a2a9b1}.mw-parser-output .ambox .mbox-text{border:none;padding:0.25em 0.5em;width:100%}.mw-parser-output .ambox .mbox-image{border:none;padding:2px 0 2px 0.5em;text-align:center}.mw-parser-output .ambox .mbox-imageright{border:none;padding:2px 0.5em 2px 0;text-align:center}.mw-parser-output .ambox .mbox-empty-cell{border:none;padding:0;width:1px}.mw-parser-output .ambox .mbox-image-div{width:52px}@media(min-width:720px){.mw-parser-output .ambox{margin:0 10%}}@media print{body.ns-0 .mw-parser-output .ambox{display:none!important}}</style><table class="box-Unreferenced_section plainlinks metadata ambox ambox-content ambox-Unreferenced" role="presentation"><tbody><tr><td class="mbox-image"><div class="mbox-image-div"><a href="/wiki/File:Question_book-new.svg" class="mw-file-description"><img alt="" src="//upload.wikimedia.org/wikipedia/en/thumb/9/99/Question_book-new.svg/50px-Question_book-new.svg.png" decoding="async" width="50" height="39" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/en/thumb/9/99/Question_book-new.svg/75px-Question_book-new.svg.png 1.5x, //upload.wikimedia.org/wikipedia/en/thumb/9/99/Question_book-new.svg/100px-Question_book-new.svg.png 2x" data-file-width="512" data-file-height="399" /></a></div></td><td class="mbox-text"><div class="mbox-text-span">This section does not <a href="/wiki/Wikipedia:Citing_sources" title="Wikipedia:Citing sources">cite</a> any <a href="/wiki/Wikipedia:Verifiability" title="Wikipedia:Verifiability">sources</a>. Please help <a href="/wiki/Special:EditPage/Raman_spectroscopy" title="Special:EditPage/Raman spectroscopy">improve this section</a> by <a href="/wiki/Help:Referencing_for_beginners" title="Help:Referencing for beginners">adding citations to reliable sources</a>. Unsourced material may be challenged and <a href="/wiki/Wikipedia:Verifiability#Burden_of_evidence" title="Wikipedia:Verifiability">removed</a>. (July 2018) (<a href="/wiki/Help:Maintenance_template_removal" title="Help:Maintenance template removal">Learn how and when to remove this message</a>)</div></td></tr></tbody></table> The magnitude of the Raman effect correlates with polarizability of the <a href="/wiki/Electron" title="Electron">electrons</a> in a molecule. It is a form of inelastic <a href="/wiki/Light_scattering" class="mw-redirect" title="Light scattering">light scattering</a>, where a <a href="/wiki/Photon" title="Photon">photon</a> excites the sample. This excitation puts the molecule into a <a href="/wiki/Virtual_state_(physics)" class="mw-redirect" title="Virtual state (physics)">virtual energy state</a> for a short time before the photon is emitted. Inelastic scattering means that the energy of the emitted photon is of either lower or higher energy than the incident photon. After the scattering event, the sample is in a different <a href="/wiki/Rigid_rotor#Quantum_mechanical_rigid_rotor" title="Rigid rotor">rotational</a> or <a href="/wiki/Molecular_vibration" title="Molecular vibration">vibrational state</a>. For the total energy of the system to remain constant after the molecule moves to a new <a href="/wiki/Rovibronic_coupling" title="Rovibronic coupling">rovibronic</a> (rotational–vibrational–electronic) state, the scattered photon shifts to a different energy, and therefore a different frequency. This energy difference is equal to that between the initial and final rovibronic states of the molecule. If the final state is higher in energy than the initial state, the scattered photon will be shifted to a lower frequency (lower energy) so that the total energy remains the same. This shift in frequency is called a <a href="/wiki/Stokes_shift" title="Stokes shift">Stokes shift</a>, or downshift. If the final state is lower in energy, the scattered photon will be shifted to a higher frequency, which is called an anti-Stokes shift, or upshift. For a molecule to exhibit a Raman effect, there must be a change in its electric dipole-electric dipole polarizability with respect to the vibrational coordinate corresponding to the rovibronic state. The intensity of the Raman scattering is proportional to this polarizability change. Therefore, the Raman spectrum (scattering intensity as a function of the frequency shifts) depends on the rovibronic states of the molecule.<a href="#cite_note-7">[7]</a> The Raman effect is based on the interaction between the electron cloud of a sample and the external electric field of the monochromatic light, which can create an induced dipole moment within the molecule based on its polarizability. Because the laser light does not excite the molecule there can be no real transition between energy levels.<a href="#cite_note-8">[8]</a> The Raman effect should not be confused with emission (<a href="/wiki/Fluorescence" title="Fluorescence">fluorescence</a> or <a href="/wiki/Phosphorescence" title="Phosphorescence">phosphorescence</a>), where a molecule in an excited electronic state emits a photon and returns to the ground electronic state, in many cases to a vibrationally excited state on the ground electronic state potential energy surface. Raman scattering also contrasts with infrared (IR) absorption, where the energy of the absorbed photon matches the difference in energy between the initial and final rovibronic states. The dependence of Raman on the electric dipole-electric dipole polarizability derivative also differs from IR spectroscopy, which depends on the electric dipole moment derivative, the atomic polar tensor (APT). This contrasting feature allows rovibronic transitions that might not be active in IR to be analyzed using Raman spectroscopy, as exemplified by the <a href="/wiki/Rule_of_mutual_exclusion" title="Rule of mutual exclusion">rule of mutual exclusion</a> in <a href="/wiki/Centrosymmetry" title="Centrosymmetry">centrosymmetric molecules</a>. Transitions which have large Raman intensities often have weak IR intensities and vice versa. If a bond is strongly polarized, a small change in its length such as that which occurs during a vibration has only a small resultant effect on polarization. Vibrations involving polar bonds (e.g. C-O , N-O , O-H) are therefore, comparatively weak Raman scatterers. Such polarized bonds, however, carry their electrical charges during the vibrational motion, (unless neutralized by symmetry factors), and this results in a larger net dipole moment change during the vibration, producing a strong IR absorption band. Conversely, relatively neutral bonds (e.g. C-C , C-H , C=C) suffer large changes in polarizability during a vibration. However, the dipole moment is not similarly affected such that while vibrations involving predominantly this type of bond are strong Raman scatterers, they are weak in the IR. A third vibrational spectroscopy technique, inelastic incoherent neutron scattering (IINS), can be used to determine the frequencies of vibrations in highly symmetric molecules that may be both IR and Raman inactive. The IINS selection rules, or allowed transitions, differ from those of IR and Raman, so the three techniques are complementary. They all give the same frequency for a given vibrational transition, but the relative intensities provide different information due to the different types of interaction between the molecule and the incoming particles, photons for IR and Raman, and neutrons for IINS. <div class="mw-heading mw-heading2"><h2 id="Raman_shift">Raman shift</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=3" title="Edit section: Raman shift">edit</a>]</div> Raman shifts are typically reported in <a href="/wiki/Wavenumber" title="Wavenumber">wavenumbers</a>, which have units of inverse length, as this value is directly related to energy. In order to convert between spectral wavelength and wavenumbers of shift in the Raman spectrum, the following formula can be used: <dl><dd><math xmlns="http://www.w3.org/1998/Math/MathML" alttext="{\displaystyle \Delta {\tilde {\nu }}=\left({\frac {1}{\lambda _{0}}}-{\frac {1}{\lambda _{1}}}\right)\ ,}"> <semantics> <mrow class="MJX-TeXAtom-ORD"> <mstyle displaystyle="true" scriptlevel="0"> <mi mathvariant="normal">Δ</mi> <mrow class="MJX-TeXAtom-ORD"> <mrow class="MJX-TeXAtom-ORD"> <mover> <mi>ν</mi> <mo stretchy="false">~</mo> </mover> </mrow> </mrow> <mo>=</mo> <mrow> <mo>(</mo> <mrow> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <mn>1</mn> <msub> <mi>λ</mi> <mrow class="MJX-TeXAtom-ORD"> <mn>0</mn> </mrow> </msub> </mfrac> </mrow> <mo>−</mo> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <mn>1</mn> <msub> <mi>λ</mi> <mrow class="MJX-TeXAtom-ORD"> <mn>1</mn> </mrow> </msub> </mfrac> </mrow> </mrow> <mo>)</mo> </mrow> <mtext> </mtext> <mo>,</mo> </mstyle> </mrow> <annotation encoding="application/x-tex">{\displaystyle \Delta {\tilde {\nu }}=\left({\frac {1}{\lambda _{0}}}-{\frac {1}{\lambda _{1}}}\right)\ ,}</annotation> </semantics> </math><img src="https://wikimedia.org/api/rest_v1/media/math/render/svg/cc228c95c2d4ae30b8606c5b0d9f22433128ca52" class="mwe-math-fallback-image-inline mw-invert skin-invert" aria-hidden="true" style="vertical-align: -2.505ex; width:20.718ex; height:6.176ex;" alt="{\displaystyle \Delta {\tilde {\nu }}=\left({\frac {1}{\lambda _{0}}}-{\frac {1}{\lambda _{1}}}\right)\ ,}"></dd></dl> where Δν̃ is the Raman shift expressed in wavenumber, λ0 is the excitation wavelength, and λ1 is the Raman spectrum wavelength. Most commonly, the unit chosen for expressing wavenumber in Raman spectra is inverse centimeters (cm−1). Since wavelength is often expressed in units of nanometers (nm), the formula above can scale for this unit conversion explicitly, giving <dl><dd><math xmlns="http://www.w3.org/1998/Math/MathML" alttext="{\displaystyle \Delta {\tilde {\nu }}({\text{cm}}^{-1})=\left({\frac {1}{\lambda _{0}({\text{nm}})}}-{\frac {1}{\lambda _{1}({\text{nm}})}}\right)\times {\frac {(10^{7}{\text{nm}})}{({\text{cm}})}}.}"> <semantics> <mrow class="MJX-TeXAtom-ORD"> <mstyle displaystyle="true" scriptlevel="0"> <mi mathvariant="normal">Δ</mi> <mrow class="MJX-TeXAtom-ORD"> <mrow class="MJX-TeXAtom-ORD"> <mover> <mi>ν</mi> <mo stretchy="false">~</mo> </mover> </mrow> </mrow> <mo stretchy="false">(</mo> <msup> <mrow class="MJX-TeXAtom-ORD"> <mtext>cm</mtext> </mrow> <mrow class="MJX-TeXAtom-ORD"> <mo>−</mo> <mn>1</mn> </mrow> </msup> <mo stretchy="false">)</mo> <mo>=</mo> <mrow> <mo>(</mo> <mrow> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <mn>1</mn> <mrow> <msub> <mi>λ</mi> <mrow class="MJX-TeXAtom-ORD"> <mn>0</mn> </mrow> </msub> <mo stretchy="false">(</mo> <mrow class="MJX-TeXAtom-ORD"> <mtext>nm</mtext> </mrow> <mo stretchy="false">)</mo> </mrow> </mfrac> </mrow> <mo>−</mo> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <mn>1</mn> <mrow> <msub> <mi>λ</mi> <mrow class="MJX-TeXAtom-ORD"> <mn>1</mn> </mrow> </msub> <mo stretchy="false">(</mo> <mrow class="MJX-TeXAtom-ORD"> <mtext>nm</mtext> </mrow> <mo stretchy="false">)</mo> </mrow> </mfrac> </mrow> </mrow> <mo>)</mo> </mrow> <mo>×</mo> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <mrow> <mo stretchy="false">(</mo> <msup> <mn>10</mn> <mrow class="MJX-TeXAtom-ORD"> <mn>7</mn> </mrow> </msup> <mrow class="MJX-TeXAtom-ORD"> <mtext>nm</mtext> </mrow> <mo stretchy="false">)</mo> </mrow> <mrow> <mo stretchy="false">(</mo> <mrow class="MJX-TeXAtom-ORD"> <mtext>cm</mtext> </mrow> <mo stretchy="false">)</mo> </mrow> </mfrac> </mrow> <mo>.</mo> </mstyle> </mrow> <annotation encoding="application/x-tex">{\displaystyle \Delta {\tilde {\nu }}({\text{cm}}^{-1})=\left({\frac {1}{\lambda _{0}({\text{nm}})}}-{\frac {1}{\lambda _{1}({\text{nm}})}}\right)\times {\frac {(10^{7}{\text{nm}})}{({\text{cm}})}}.}</annotation> </semantics> </math><img src="https://wikimedia.org/api/rest_v1/media/math/render/svg/8cc43b06db406d3d79c322eb5e395da742fc9754" class="mwe-math-fallback-image-inline mw-invert skin-invert" aria-hidden="true" style="vertical-align: -2.671ex; width:49.029ex; height:6.676ex;" alt="{\displaystyle \Delta {\tilde {\nu }}({\text{cm}}^{-1})=\left({\frac {1}{\lambda _{0}({\text{nm}})}}-{\frac {1}{\lambda _{1}({\text{nm}})}}\right)\times {\frac {(10^{7}{\text{nm}})}{({\text{cm}})}}.}"></dd></dl> <div class="mw-heading mw-heading2"><h2 id="Instrumentation">Instrumentation</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=4" title="Edit section: Instrumentation">edit</a>]</div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:1928_Benzene_Raman_Spectrum.png" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/d/dd/1928_Benzene_Raman_Spectrum.png/220px-1928_Benzene_Raman_Spectrum.png" decoding="async" width="220" height="119" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/d/dd/1928_Benzene_Raman_Spectrum.png/330px-1928_Benzene_Raman_Spectrum.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/d/dd/1928_Benzene_Raman_Spectrum.png/440px-1928_Benzene_Raman_Spectrum.png 2x" data-file-width="580" data-file-height="314" /></a><figcaption>An early Raman spectrum of <a href="/wiki/Benzene" title="Benzene">benzene</a> published by Raman and Krishnan.<a href="#cite_note-9">[9]</a></figcaption></figure> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2(2)_(2019)_1662-1683.png" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/d/db/Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683.png/220px-Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683.png" decoding="async" width="220" height="338" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/d/db/Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683.png/330px-Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/d/db/Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683.png/440px-Setup_Raman_Spectroscopy_adapted_from_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683.png 2x" data-file-width="873" data-file-height="1343" /></a><figcaption>Schematic of one possible dispersive Raman spectroscopy setup.<a href="#cite_note-Heritage-10">[10]</a></figcaption></figure> Modern Raman spectroscopy nearly always involves the use of <a href="/wiki/Laser" title="Laser">lasers</a> as excitation light sources. Because lasers were not available until more than three decades after the discovery of the effect, Raman and Krishnan used a <a href="/wiki/Mercury_lamp" class="mw-redirect" title="Mercury lamp">mercury lamp</a> and <a href="/wiki/Photographic_plate" title="Photographic plate">photographic plates</a> to record spectra. Early spectra took hours or even days to acquire due to weak light sources, poor sensitivity of the detectors and the weak Raman scattering cross-sections of most materials. Various colored filters and chemical solutions were used to select certain wavelength regions for excitation and detection but the photographic spectra were still dominated by a broad center line corresponding to Rayleigh scattering of the excitation source.<a href="#cite_note-Long-11">[11]</a> Technological advances have made Raman spectroscopy much more sensitive, particularly since the 1980s. The most common modern detectors are now <a href="/wiki/Charge-coupled_devices" class="mw-redirect" title="Charge-coupled devices">charge-coupled devices</a> (CCDs). <a href="/wiki/Photodiode_array" class="mw-redirect" title="Photodiode array">Photodiode arrays</a> and <a href="/wiki/Photomultiplier_tube" title="Photomultiplier tube">photomultiplier tubes</a> were common prior to the adoption of CCDs. The advent of reliable, stable, inexpensive lasers with narrow bandwidths has also had an impact.<a href="#cite_note-McCreery-12">[12]</a> <div class="mw-heading mw-heading3"><h3 id="Lasers">Lasers</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=5" title="Edit section: Lasers">edit</a>]</div> Raman spectroscopy requires a light source such as a laser. The resolution of the spectrum relies on the bandwidth of the laser source used.<a href="#cite_note-MathiesFSRS-13">[13]</a> Generally shorter wavelength lasers give stronger Raman scattering due to the ν4 increase in Raman scattering cross-sections, but issues with sample degradation or fluorescence may result.<a href="#cite_note-McCreery-12">[12]</a> <a href="/wiki/Continuous_wave" title="Continuous wave">Continuous wave</a> lasers are most common for normal Raman spectroscopy, but <a href="/wiki/Pulsed_laser" title="Pulsed laser">pulsed lasers</a> may also be used. These often have wider bandwidths than their CW counterparts but are very useful for other forms of Raman spectroscopy such as transient, time-resolved and resonance Raman.<a href="#cite_note-MathiesFSRS-13">[13]</a><a href="#cite_note-KCG-14">[14]</a> <div class="mw-heading mw-heading3"><h3 id="Detectors">Detectors</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=6" title="Edit section: Detectors">edit</a>]</div> Raman scattered light is typically collected and either dispersed by a <a href="/wiki/Spectrograph" class="mw-redirect" title="Spectrograph">spectrograph</a> or used with an <a href="/wiki/Interferometer" class="mw-redirect" title="Interferometer">interferometer</a> for detection by Fourier Transform (FT) methods. In many cases commercially available FT-IR spectrometers can be modified to become FT-Raman spectrometers.<a href="#cite_note-McCreery-12">[12]</a> <div class="mw-heading mw-heading4"><h4 id="Detectors_for_dispersive_Raman">Detectors for dispersive Raman</h4>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=7" title="Edit section: Detectors for dispersive Raman">edit</a>]</div> In most cases, modern Raman spectrometers use array detectors such as CCDs. Various types of CCDs exist which are optimized for different wavelength ranges. <a href="/wiki/Intensified_CCD" class="mw-redirect" title="Intensified CCD">Intensified CCDs</a> can be used for very weak signals and/or pulsed lasers.<a href="#cite_note-McCreery-12">[12]</a><a href="#cite_note-15">[15]</a> The spectral range depends on the size of the CCD and the focal length of spectrograph used.<a href="#cite_note-16">[16]</a> It was once common to use <a href="/wiki/Monochromator" title="Monochromator">monochromators</a> coupled to photomultiplier tubes. In this case the monochromator would need to be moved in order to scan through a spectral range.<a href="#cite_note-McCreery-12">[12]</a> <div class="mw-heading mw-heading4"><h4 id="Detectors_for_FT–Raman">Detectors for FT–Raman</h4>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=8" title="Edit section: Detectors for FT–Raman">edit</a>]</div> FT–Raman is almost always used with NIR lasers and appropriate detectors must be used depending on the exciting wavelength. <a href="/wiki/Germanium_detector" class="mw-redirect" title="Germanium detector">Germanium</a> or <a href="/wiki/Indium_gallium_arsenide" title="Indium gallium arsenide">Indium gallium arsenide</a> (InGaAs) detectors are commonly used.<a href="#cite_note-McCreery-12">[12]</a> <div class="mw-heading mw-heading3"><h3 id="Filters">Filters</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=9" title="Edit section: Filters">edit</a>]</div> It is usually necessary to separate the Raman scattered light from the Rayleigh signal and reflected laser signal in order to collect high quality Raman spectra using a laser rejection filter. <a href="/wiki/Notch_filter" class="mw-redirect" title="Notch filter">Notch</a> or <a href="/wiki/Long-pass_filter" class="mw-redirect" title="Long-pass filter">long-pass</a> optical filters are typically used for this purpose. Before the advent of holographic filters it was common to use a triple-grating monochromator in subtractive mode to isolate the desired signal.<a href="#cite_note-McCreery-12">[12]</a> This may still be used to record very small Raman shifts as holographic filters typically reflect some of the low frequency bands in addition to the unshifted laser light. However, <a href="/wiki/Volume_hologram" title="Volume hologram">Volume hologram</a> filters are becoming more common which allow shifts as low as 5 cm−1 to be observed.<a href="#cite_note-17">[17]</a><a href="#cite_note-18">[18]</a><a href="#cite_note-19">[19]</a> <div class="mw-heading mw-heading2"><h2 id="Applications">Applications</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=10" title="Edit section: Applications">edit</a>]</div> Raman spectroscopy is used in chemistry to identify molecules and study chemical bonding and intramolecular bonds. Because vibrational frequencies are specific to a molecule's <a href="/wiki/Chemical_bond" title="Chemical bond">chemical bonds</a> and symmetry (the fingerprint region of organic molecules is in the <a href="/wiki/Wavenumber" title="Wavenumber">wavenumber</a> range 500–1,500 cm−1),<a href="#cite_note-20">[20]</a> Raman provides a fingerprint to identify molecules. For instance, Raman and IR spectra were used to determine the vibrational frequencies of SiO, Si2O2, and Si3O3 on the basis of normal coordinate analyses.<a href="#cite_note-R._K._Khanna_and_D._D._Stranz-21">[21]</a> Raman is also used to study the addition of a substrate to an enzyme. In <a href="/wiki/Solid-state_physics" title="Solid-state physics">solid-state physics</a>, Raman spectroscopy is used to characterize materials, measure <a href="/wiki/Temperature" title="Temperature">temperature</a>, and find the crystallographic orientation of a sample. As with single molecules, a solid material can be identified by characteristic <a href="/wiki/Phonon" title="Phonon">phonon</a> modes. Information on the population of a phonon mode is given by the ratio of the Stokes and anti-Stokes intensity of the spontaneous Raman signal. Raman spectroscopy can also be used to observe other low frequency excitations of a solid, such as <a href="/wiki/Plasmon" title="Plasmon">plasmons</a>, <a href="/wiki/Magnon" title="Magnon">magnons</a>, and <a href="/wiki/BCS_theory" title="BCS theory">superconducting gap</a> excitations. <a href="/wiki/Distributed_temperature_sensing" title="Distributed temperature sensing">Distributed temperature sensing</a> (DTS) uses the Raman-shifted backscatter from laser pulses to determine the temperature along optical fibers. The orientation of an anisotropic <a href="/wiki/Crystal" title="Crystal">crystal</a> can be found from the <a href="/wiki/Polarization_(waves)" title="Polarization (waves)">polarization</a> of Raman-scattered light with respect to the crystal and the polarization of the laser light, if the <a href="/wiki/Crystal_structure" title="Crystal structure">crystal structure</a>’s <a href="/wiki/Crystallographic_point_group" title="Crystallographic point group">point group</a> is known. <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Raman_Microscope.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Raman_Microscope.jpg/220px-Raman_Microscope.jpg" decoding="async" width="220" height="167" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Raman_Microscope.jpg/330px-Raman_Microscope.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Raman_Microscope.jpg/440px-Raman_Microscope.jpg 2x" data-file-width="2254" data-file-height="1716" /></a><figcaption>Raman microscope at the Chemistry Department Shared Instrumentation Facility (NYU).</figcaption></figure> In nanotechnology, a Raman microscope can be used to analyze nanowires to better understand their structures, and the radial breathing mode of carbon nanotubes is commonly used to evaluate their diameter. Raman active fibers, such as <a href="/wiki/Aramid" title="Aramid">aramid</a> and carbon, have vibrational modes that show a shift in Raman frequency with applied stress. <a href="/wiki/Polypropylene" title="Polypropylene">Polypropylene</a> fibers exhibit similar shifts. In solid state chemistry and the bio-pharmaceutical industry, Raman spectroscopy can be used to not only identify active pharmaceutical ingredients (APIs), but to identify their polymorphic forms, if more than one exist. For example, the drug <a href="/wiki/Cayston" class="mw-redirect" title="Cayston">Cayston</a> (<a href="/wiki/Aztreonam" title="Aztreonam">aztreonam</a>), marketed by <a href="/wiki/Gilead_Sciences" title="Gilead Sciences">Gilead Sciences</a> for <a href="/wiki/Cystic_fibrosis" title="Cystic fibrosis">cystic fibrosis</a>,<a href="#cite_note-22">[22]</a> can be identified and characterized by IR and Raman spectroscopy. Using the correct polymorphic form in bio-pharmaceutical formulations is critical, since different forms have different physical properties, like solubility and melting point. Raman spectroscopy has a wide variety of applications in biology and medicine. It has helped confirm the existence of low-frequency phonons<a href="#cite_note-23">[23]</a> in proteins and DNA,<a href="#cite_note-24">[24]</a><a href="#cite_note-25">[25]</a><a href="#cite_note-26">[26]</a><a href="#cite_note-27">[27]</a> promoting studies of <a href="/w/index.php?title=Low-frequency_collective_motion_in_proteins_and_DNA&action=edit&redlink=1" class="new" title="Low-frequency collective motion in proteins and DNA (page does not exist)">low-frequency collective motion in proteins and DNA</a> and their biological functions.<a href="#cite_note-28">[28]</a><a href="#cite_note-29">[29]</a> Raman reporter molecules with <a href="/wiki/Olefin" class="mw-redirect" title="Olefin">olefin</a> or <a href="/wiki/Alkyne" title="Alkyne">alkyne</a> moieties are being developed for tissue imaging with SERS-labeled <a href="/wiki/Antibodies" class="mw-redirect" title="Antibodies">antibodies</a>.<a href="#cite_note-30">[30]</a> Raman spectroscopy has also been used as a noninvasive technique for real-time, in situ biochemical characterization of wounds. Multivariate analysis of Raman spectra has enabled development of a quantitative measure for wound healing progress.<a href="#cite_note-31">[31]</a> <a href="/wiki/Spatially_offset_Raman_spectroscopy" title="Spatially offset Raman spectroscopy">Spatially offset Raman spectroscopy</a> (SORS), which is less sensitive to surface layers than conventional Raman, can be used to discover <a href="/wiki/Counterfeit_drug" class="mw-redirect" title="Counterfeit drug">counterfeit drugs</a> without opening their packaging, and to non-invasively study biological tissue.<a href="#cite_note-32">[32]</a> A reason why Raman spectroscopy is useful in biological applications is because its results often do not face interference from water molecules, due to the fact that they have permanent dipole moments, and as a result, the Raman scattering cannot be picked up on. This is a large advantage, specifically in biological applications.<a href="#cite_note-33">[33]</a> Raman spectroscopy also has a wide usage for studying biominerals.<a href="#cite_note-TaylorVinnKudryavtsevSchopf2010-34">[34]</a> Lastly, Raman gas analyzers have many practical applications, including real-time monitoring of anesthetic and respiratory gas mixtures during surgery. Raman spectroscopy has been used in several research projects as a means to detect <a href="/wiki/Explosive" title="Explosive">explosives</a> from a safe distance using laser beams.<a href="#cite_note-35">[35]</a><a href="#cite_note-36">[36]</a><a href="#cite_note-autogenerated1-37">[37]</a> Raman Spectroscopy is being further developed so it could be used in the clinical setting. Raman4Clinic is a European organization that is working on incorporating Raman Spectroscopy techniques in the medical field. They are currently working on different projects, one of them being monitoring cancer using bodily fluids such as urine and blood samples which are easily accessible. This technique would be less stressful on the patients than constantly having to take biopsies which are not always risk free.<a href="#cite_note-38">[38]</a> In <a href="/wiki/Photovoltaics" title="Photovoltaics">photovoltaics</a>, Raman spectroscopy has gained more interest in the past few years demonstrating high efficacy in delivering important properties for such materials. This includes optoelectronic and physicochemical properties such as open circuit voltage, efficiency, and crystalline structure.<a href="#cite_note-grauluque2021-39">[39]</a> This has been demonstrated with several photovoltaic technologies, including kesterite-based,<a href="#cite_note-grauluque2021-39">[39]</a> <a href="/wiki/Copper_indium_gallium_selenide_solar_cell" title="Copper indium gallium selenide solar cell">CIGS devices</a>,<a href="#cite_note-40">[40]</a> <a href="/wiki/Monocrystalline_silicon" title="Monocrystalline silicon">Monocrystalline silicon</a> cells,<a href="#cite_note-41">[41]</a> and <a href="/wiki/Perovskite_solar_cell" title="Perovskite solar cell">perovskites devices</a>.<a href="#cite_note-42">[42]</a> <div class="mw-heading mw-heading3"><h3 id="Art_and_cultural_heritage">Art and cultural heritage</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=11" title="Edit section: Art and cultural heritage">edit</a>]</div> Raman spectroscopy is an efficient and non-destructive way to investigate works of art and <a href="/wiki/Cultural_heritage" title="Cultural heritage">cultural heritage</a> artifacts, in part because it is a non-invasive process which can be applied <a href="/wiki/In_situ" title="In situ">in situ</a>.<a href="#cite_note-43">[43]</a> It can be used to analyze the corrosion products on the surfaces of artifacts (statues, pottery, etc.), which can lend insight into the corrosive environments experienced by the artifacts. The resulting spectra can also be compared to the spectra of surfaces that are cleaned or intentionally corroded, which can aid in determining the authenticity of valuable historical artifacts.<a href="#cite_note-44">[44]</a> It is capable of identifying individual pigments in paintings and their degradation products, which can provide insight into the working method of an artist in addition to aiding in authentication of paintings.<a href="#cite_note-45">[45]</a> It also gives information about the original state of the painting in cases where the pigments have degraded with age.<a href="#cite_note-46">[46]</a> Beyond the identification of pigments, extensive Raman microspectroscopic imaging has been shown to provide access to a plethora of trace compounds in Early Medieval <a href="/wiki/Egyptian_blue" title="Egyptian blue">Egyptian blue</a>, which enable to reconstruct the individual "biography" of a colourant, including information on the type and provenance of the raw materials, synthesis and application of the pigment, and the ageing of the paint layer.<a href="#cite_note-NaturePortfolio-47">[47]</a> In addition to paintings and artifacts, Raman spectroscopy can be used to investigate the chemical composition of historical documents (such as the <a href="/wiki/Book_of_Kells" title="Book of Kells">Book of Kells</a>), which can provide insight about the social and economic conditions when they were created.<a href="#cite_note-48">[48]</a> It also offers a noninvasive way to determine the best method of <a href="/wiki/Preservation_(library_and_archival_science)" class="mw-redirect" title="Preservation (library and archival science)">preservation</a> or <a href="/wiki/Art_conservation" class="mw-redirect" title="Art conservation">conservation</a> of such cultural heritage artifacts, by providing insight into the causes behind deterioration.<a href="#cite_note-49">[49]</a> The IRUG (Infrared and Raman Users Group) Spectral Database<a href="#cite_note-50">[50]</a> is a rigorously peer-reviewed online database of IR and Raman reference spectra for cultural heritage materials such as works of art, architecture, and archaeological artifacts. The database is open for the general public to peruse, and includes interactive spectra for over a hundred different types of pigments and paints. <div class="mw-heading mw-heading2"><h2 id="Microspectroscopy">Microspectroscopy</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=12" title="Edit section: Microspectroscopy">edit</a>]</div> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1236090951"><div role="note" class="hatnote navigation-not-searchable">Main article: <a href="/wiki/Raman_microscope" title="Raman microscope">Raman microscope</a></div> <figure typeof="mw:File/Thumb"><a href="/wiki/File:Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2(2)_(2019)_1662-1683_landscape.png" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/f/fb/Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683_landscape.png/400px-Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683_landscape.png" decoding="async" width="400" height="358" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/f/fb/Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683_landscape.png/600px-Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683_landscape.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/f/fb/Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683_landscape.png/800px-Raman_cement_clinker_remnant_FigTOC_Thomas_Schmid_and_Petra_Dariz_in_Heritage_2%282%29_%282019%29_1662-1683_landscape.png 2x" data-file-width="1024" data-file-height="917" /></a><figcaption>Hyperspectral Raman imaging can provide distribution maps of chemical compounds and material properties: Example of an unhydrated <a href="/wiki/Clinker_(cement)" class="mw-redirect" title="Clinker (cement)">clinker</a> remnant in a 19th-century <a href="/wiki/Cement" title="Cement">cement</a> mortar (cement chemist's nomenclature: C ≙ CaO, A ≙ Al2O3, S ≙ SiO2, F ≙ Fe2O3).<a href="#cite_note-Heritage-10">[10]</a></figcaption></figure> Raman spectroscopy offers several advantages for <a href="/wiki/Microscopy" title="Microscopy">microscopic</a> analysis. Since it is a light scattering technique, specimens do not need to be fixed or sectioned. Raman spectra can be collected from a very small volume (< 1 μm in diameter, < 10 μm in depth); these spectra allow the identification of species present in that volume.<a href="#cite_note-AnnuRevAnalChem-51">[51]</a> Water does not generally interfere with Raman spectral analysis. Thus, Raman spectroscopy is suitable for the microscopic examination of <a href="/wiki/Mineral" title="Mineral">minerals</a>, materials such as polymers and ceramics, <a href="/wiki/Cell_(biology)" title="Cell (biology)">cells</a>, <a href="/wiki/Proteins" class="mw-redirect" title="Proteins">proteins</a> and forensic trace evidence. A <a href="/wiki/Raman_microscope" title="Raman microscope">Raman microscope</a> begins with a standard optical microscope, and adds an excitation laser, a <a href="/wiki/Monochromator" title="Monochromator">monochromator</a> or <a href="/wiki/Polychromator" title="Polychromator">polychromator</a>, and a sensitive detector (such as a <a href="/wiki/Charge-coupled_device" title="Charge-coupled device">charge-coupled device</a> (CCD), or <a href="/wiki/Photomultiplier" title="Photomultiplier">photomultiplier</a> tube (PMT)). <a href="/wiki/Fourier_transform_spectroscopy" class="mw-redirect" title="Fourier transform spectroscopy">FT-Raman</a> has also been used with microscopes, typically in combination with <a href="/wiki/Near-infrared" class="mw-redirect" title="Near-infrared">near-infrared</a> (NIR) laser excitation. <a href="/wiki/Microscopy#Ultraviolet_microscopy" title="Microscopy">Ultraviolet microscopes</a> and UV enhanced optics must be used when a UV laser source is used for Raman microspectroscopy. In direct imaging (also termed global imaging<a href="#cite_note-52">[52]</a> or wide-field illumination), the whole field of view is examined for light scattering integrated over a small range of wavenumbers (Raman shifts).<a href="#cite_note-AnalChem-53">[53]</a> For instance, a wavenumber characteristic for cholesterol could be used to record the distribution of cholesterol within a cell culture. This technique is being used for the characterization of large-scale devices, mapping of different compounds and dynamics study. It has already been used for the characterization of <a href="/wiki/Graphene" title="Graphene">graphene</a> layers,<a href="#cite_note-54">[54]</a> J-aggregated dyes inside <a href="/wiki/Carbon_nanotubes" class="mw-redirect" title="Carbon nanotubes">carbon nanotubes</a><a href="#cite_note-55">[55]</a> and multiple other 2D materials such as <a href="/wiki/Molybdenum_disulfide" title="Molybdenum disulfide">MoS2</a> and <a href="/wiki/Tungsten_diselenide" title="Tungsten diselenide">WSe2</a>. Since the excitation beam is dispersed over the whole field of view, those measurements can be done without damaging the sample. The most common approach is <a href="/wiki/Hyperspectral_imaging" title="Hyperspectral imaging">hyperspectral imaging</a> or <a href="/wiki/Chemical_imaging" title="Chemical imaging">chemical imaging</a>, in which thousands of Raman spectra are acquired from all over the field of view by, for example, raster scanning of a focused laser beam through a sample.<a href="#cite_note-AnalChem-53">[53]</a> The data can be used to generate images showing the location and amount of different components. Having the full spectroscopic information available in every measurement spot has the advantage that several components can be mapped at the same time, including chemically similar and even <a href="/wiki/Polymorphism_(materials_science)" class="mw-redirect" title="Polymorphism (materials science)">polymorphic</a> forms, which cannot be distinguished by detecting only one single wavenumber. Furthermore, material properties such as <a href="/wiki/Stress_(mechanics)" title="Stress (mechanics)">stress</a> and <a href="/wiki/Strain_(mechanics)" title="Strain (mechanics)">strain</a>, <a href="/wiki/Orientation_imaging_microscopy" class="mw-redirect" title="Orientation imaging microscopy">crystal orientation</a>, <a href="/wiki/Crystallinity" title="Crystallinity">crystallinity</a> and incorporation of foreign ions into crystal lattices (e.g., <a href="/wiki/Doping_(semiconductor)" title="Doping (semiconductor)">doping</a>, <a href="/wiki/Solid_solution" title="Solid solution">solid solution series</a>) can be determined from hyperspectral maps.<a href="#cite_note-Heritage-10">[10]</a> Taking the cell culture example, a hyperspectral image could show the distribution of cholesterol, as well as proteins, nucleic acids, and fatty acids. Sophisticated signal- and image-processing techniques can be used to ignore the presence of water, culture media, buffers, and other interferences. Because a Raman microscope is a <a href="/wiki/Diffraction-limited_system" title="Diffraction-limited system">diffraction-limited system</a>, its spatial resolution depends on the wavelength of light, the <a href="/wiki/Numerical_aperture" title="Numerical aperture">numerical aperture</a> of the focusing element, and — in the case of <a href="/wiki/Confocal_microscopy" title="Confocal microscopy">confocal microscopy</a> — on the diameter of the confocal aperture. When operated in the visible to near-infrared range, a Raman microscope can achieve lateral resolutions of approx. 1 μm down to 250 nm, depending on the wavelength and type of objective lens (e.g., air vs. water or oil immersion lenses). The depth resolution (if not limited by the optical penetration depth of the sample) can range from 1–6 μm with the smallest confocal pinhole aperture to tens of micrometers when operated without a confocal pinhole.<a href="#cite_note-56">[56]</a><a href="#cite_note-ApplSpectrosc-57">[57]</a><a href="#cite_note-58">[58]</a><a href="#cite_note-AnnuRevAnalChem-51">[51]</a> Depending on the sample, the high laser power density due to microscopic focussing can have the benefit of enhanced <a href="/wiki/Photobleaching" title="Photobleaching">photobleaching</a> of molecules emitting interfering fluorescence. However, the laser wavelength and laser power have to be carefully selected for each type of sample to avoid its degradation. Applications of Raman imaging range from materials sciences to biological studies.<a href="#cite_note-AnnuRevAnalChem-51">[51]</a><a href="#cite_note-59">[59]</a> For each type of sample, the measurement parameters have to be individually optimized. For that reason, modern Raman microscopes are often equipped with several lasers offering different wavelengths, a set of objective lenses, and neutral density filters for tuning of the laser power reaching the sample. Selection of the laser wavelength mainly depends on optical properties of the sample and on the aim of the investigation.<a href="#cite_note-60">[60]</a> For example, Raman microscopy of biological and medical specimens is often performed using red to near-infrared excitation (e.g., 785 nm, or 1,064 nm wavelength). Due to typically low <a href="/wiki/Absorbance" title="Absorbance">absorbances</a> of biological samples in this spectral range, the risk of damaging the specimen as well as <a href="/wiki/Autofluorescence" title="Autofluorescence">autofluorescence</a> emission are reduced, and high penetration depths into tissues can be achieved.<a href="#cite_note-61">[61]</a><a href="#cite_note-62">[62]</a><a href="#cite_note-63">[63]</a><a href="#cite_note-64">[64]</a> However, the intensity of Raman scattering at long wavelengths is low (owing to the ω4 dependence of Raman scattering intensity), leading to long acquisition times. On the other hand, <a href="/wiki/Resonance_raman_spectroscopy" class="mw-redirect" title="Resonance raman spectroscopy">resonance Raman</a> imaging of single-cell <a href="/wiki/Algae" title="Algae">algae</a> at 532 nm (green) can specifically probe the <a href="/wiki/Carotenoid" title="Carotenoid">carotenoid</a> distribution within a cell by a using low laser power of ~5 μW and only 100 ms acquisition time.<a href="#cite_note-65">[65]</a> Raman scattering, specifically tip-enhanced Raman spectroscopy, produces high resolution hyperspectral images of single molecules,<a href="#cite_note-66">[66]</a> atoms,<a href="#cite_note-67">[67]</a> and DNA.<a href="#cite_note-He_753–757-68">[68]</a> <div class="mw-heading mw-heading2"><h2 id="Polarization_dependence_of_Raman_scattering">Polarization dependence of Raman scattering</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=13" title="Edit section: Polarization dependence of Raman scattering">edit</a>]</div> Raman scattering is polarization sensitive and can provide detailed information on symmetry of Raman active modes. While conventional Raman spectroscopy identifies chemical composition, polarization effects on Raman spectra can reveal information on the orientation of molecules in single crystals and anisotropic materials, e.g. strained plastic sheets, as well as the symmetry of vibrational modes. Polarization–dependent Raman spectroscopy uses (plane) polarized laser excitation from a <a href="/wiki/Polarizer" title="Polarizer">polarizer</a>. The Raman scattered light collected is passed through a second polarizer (called the analyzer) before entering the detector. The analyzer is oriented either parallel or perpendicular to the polarization of the laser. Spectra acquired with the analyzer set at both perpendicular and parallel to the excitation plane can be used to calculate the <a href="/wiki/Depolarization_ratio" title="Depolarization ratio">depolarization ratio</a>. Typically a <a href="/wiki/Polarization_scrambling" title="Polarization scrambling">polarization scrambler</a> is placed between the analyzer and detector also.[<a href="/wiki/Wikipedia:Citation_needed" title="Wikipedia:Citation needed">citation needed</a>]It is convenient in polarized Raman spectroscopy to describe the propagation and polarization directions using Porto's notation,<a href="#cite_note-Porto's_notation-69">[69]</a> described by and named after Brazilian physicist <a href="/wiki/Sergio_Pereira_da_Silva_Porto" class="mw-redirect" title="Sergio Pereira da Silva Porto">Sergio Pereira da Silva Porto</a>. For isotropic solutions, the Raman scattering from each mode either retains the polarization of the laser or becomes partly or fully depolarized. If the vibrational mode involved in the Raman scattering process is totally symmetric then the polarization of the Raman scattering will be the same as that of the incoming laser beam. In the case that the vibrational mode is not totally symmetric then the polarization will be lost (scrambled) partially or totally, which is referred to as depolarization. Hence polarized Raman spectroscopy can provide detailed information as to the symmetry labels of vibrational modes. In the solid state, polarized Raman spectroscopy can be useful in the study of oriented samples such as single crystals. The polarizability of a vibrational mode is not equal along and across the bond. Therefore the intensity of the Raman scattering will be different when the laser's polarization is along and orthogonal to a particular bond axis. This effect can provide information on the orientation of molecules with a single crystal or material. The spectral information arising from this analysis is often used to understand macro-molecular orientation in crystal lattices, <a href="/wiki/Liquid_crystal" title="Liquid crystal">liquid crystals</a> or polymer samples.<a href="#cite_note-Joseph_M._Grzybowski,_R._K._Khanna,_E._R._Lippincott-70">[70]</a> <div class="mw-heading mw-heading3"><h3 id="Characterization_of_the_symmetry_of_a_vibrational_mode">Characterization of the symmetry of a vibrational mode</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=14" title="Edit section: Characterization of the symmetry of a vibrational mode">edit</a>]</div> The polarization technique is useful in understanding the connections between <a href="/wiki/Molecular_symmetry" title="Molecular symmetry">molecular symmetry</a>, Raman activity, and peaks in the corresponding Raman spectra.<a href="#cite_note-71">[71]</a> Polarized light in one direction only gives access to some Raman–active modes, but rotating the polarization gives access to other modes. Each mode is separated according to its symmetry.<a href="#cite_note-72">[72]</a> The symmetry of a vibrational mode is deduced from the depolarization ratio ρ, which is the ratio of the Raman scattering with polarization orthogonal to the incident laser and the Raman scattering with the same polarization as the incident laser: <math xmlns="http://www.w3.org/1998/Math/MathML" alttext="{\displaystyle \rho ={\frac {I_{r}}{I_{u}}}}"> <semantics> <mrow class="MJX-TeXAtom-ORD"> <mstyle displaystyle="true" scriptlevel="0"> <mi>ρ</mi> <mo>=</mo> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <msub> <mi>I</mi> <mrow class="MJX-TeXAtom-ORD"> <mi>r</mi> </mrow> </msub> <msub> <mi>I</mi> <mrow class="MJX-TeXAtom-ORD"> <mi>u</mi> </mrow> </msub> </mfrac> </mrow> </mstyle> </mrow> <annotation encoding="application/x-tex">{\displaystyle \rho ={\frac {I_{r}}{I_{u}}}}</annotation> </semantics> </math><img src="https://wikimedia.org/api/rest_v1/media/math/render/svg/6b697d4c17bfa21ef98854414df7a122a9c174ff" class="mwe-math-fallback-image-inline mw-invert skin-invert" aria-hidden="true" style="vertical-align: -2.338ex; width:7.332ex; height:5.676ex;" alt="{\displaystyle \rho ={\frac {I_{r}}{I_{u}}}}"> Here <math xmlns="http://www.w3.org/1998/Math/MathML" alttext="{\displaystyle I_{r}}"> <semantics> <mrow class="MJX-TeXAtom-ORD"> <mstyle displaystyle="true" scriptlevel="0"> <msub> <mi>I</mi> <mrow class="MJX-TeXAtom-ORD"> <mi>r</mi> </mrow> </msub> </mstyle> </mrow> <annotation encoding="application/x-tex">{\displaystyle I_{r}}</annotation> </semantics> </math><img src="https://wikimedia.org/api/rest_v1/media/math/render/svg/540b248dfa84cd32dc16402b1cade5866cd6fd2f" class="mwe-math-fallback-image-inline mw-invert skin-invert" aria-hidden="true" style="vertical-align: -0.671ex; width:1.997ex; height:2.509ex;" alt="{\displaystyle I_{r}}"> is the intensity of Raman scattering when the analyzer is rotated 90 degrees with respect to the incident light's polarization axis, and <math xmlns="http://www.w3.org/1998/Math/MathML" alttext="{\displaystyle I_{u}}"> <semantics> <mrow class="MJX-TeXAtom-ORD"> <mstyle displaystyle="true" scriptlevel="0"> <msub> <mi>I</mi> <mrow class="MJX-TeXAtom-ORD"> <mi>u</mi> </mrow> </msub> </mstyle> </mrow> <annotation encoding="application/x-tex">{\displaystyle I_{u}}</annotation> </semantics> </math><img src="https://wikimedia.org/api/rest_v1/media/math/render/svg/1331605a949f78d851bac7950675dfefbc7f8afa" class="mwe-math-fallback-image-inline mw-invert skin-invert" aria-hidden="true" style="vertical-align: -0.671ex; width:2.196ex; height:2.509ex;" alt="{\displaystyle I_{u}}"> the intensity of Raman scattering when the analyzer is aligned with the polarization of the incident laser.<a href="#cite_note-Banwell-73">[73]</a> When polarized light interacts with a molecule, it distorts the molecule which induces an equal and opposite effect in the plane-wave, causing it to be rotated by the difference between the orientation of the molecule and the angle of polarization of the light wave. If <math xmlns="http://www.w3.org/1998/Math/MathML" alttext="{\textstyle \rho \geq {\frac {3}{4}}}"> <semantics> <mrow class="MJX-TeXAtom-ORD"> <mstyle displaystyle="false" scriptlevel="0"> <mi>ρ</mi> <mo>≥</mo> <mrow class="MJX-TeXAtom-ORD"> <mfrac> <mn>3</mn> <mn>4</mn> </mfrac> </mrow> </mstyle> </mrow> <annotation encoding="application/x-tex">{\textstyle \rho \geq {\frac {3}{4}}}</annotation> </semantics> </math><img src="https://wikimedia.org/api/rest_v1/media/math/render/svg/14465e30d4200d7b3411cd02282bd5e8de122e20" class="mwe-math-fallback-image-inline mw-invert skin-invert" aria-hidden="true" style="vertical-align: -1.171ex; width:5.959ex; height:3.509ex;" alt="{\textstyle \rho \geq {\frac {3}{4}}}">, then the vibrations at that frequency are depolarized; meaning they are not totally symmetric.<a href="#cite_note-74">[74]</a><a href="#cite_note-Banwell-73">[73]</a> <div class="mw-heading mw-heading3"><h3 id="Raman_Excitation_Profile_Analysis">Raman Excitation Profile Analysis</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=15" title="Edit section: Raman Excitation Profile Analysis">edit</a>]</div> Resonance Raman <a href="/wiki/Selection_rule" title="Selection rule">selection rules</a> can be explained by the Kramers-Heisenberg-Dirac (KHD) equation using the Albrecht A and B terms, as demonstrated.<a href="#cite_note-75">[75]</a> The KHD expression is conveniently linked to the polarizability of the molecule within its frame of reference.<a href="#cite_note-:0-76">[76]</a> <figure class="mw-halign-center" typeof="mw:File/Frameless"><a href="/wiki/File:Kramers-Heisenberg-Dirac_(KHD).png" class="mw-file-description" title="Kramers-Heisenberg-Dirac[76]"><img alt="Kramers-Heisenberg-Dirac[76]" src="//upload.wikimedia.org/wikipedia/commons/thumb/2/26/Kramers-Heisenberg-Dirac_%28KHD%29.png/600px-Kramers-Heisenberg-Dirac_%28KHD%29.png" decoding="async" width="600" height="176" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/2/26/Kramers-Heisenberg-Dirac_%28KHD%29.png/900px-Kramers-Heisenberg-Dirac_%28KHD%29.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/2/26/Kramers-Heisenberg-Dirac_%28KHD%29.png/1200px-Kramers-Heisenberg-Dirac_%28KHD%29.png 2x" data-file-width="1302" data-file-height="382" /></a><figcaption>Kramers-Heisenberg-Dirac<a href="#cite_note-:0-76">[76]</a></figcaption></figure> The <a href="/wiki/Polarizability" title="Polarizability">polarizability</a> operator connecting the initial and final states expresses the transition polarizability as a <a href="/wiki/Matrix_element_(physics)" title="Matrix element (physics)">matrix element</a>, as a function of the incidence frequency ω0.<a href="#cite_note-:0-76">[76]</a> The directions x, y, and z in the molecular frame are represented by the <a href="/wiki/Cartesian_tensor" title="Cartesian tensor">Cartesian tensor</a> ρ and σ here. Analyzing Raman excitation patterns requires the use of this equation, which is a sum-over-states expression for polarizability. This series of profiles illustrates the connection between a Raman active vibration's excitation <a href="/wiki/Frequency" title="Frequency">frequency</a> and <a href="/wiki/Intensity_(physics)" title="Intensity (physics)">intensity</a>.<a href="#cite_note-:0-76">[76]</a> This method takes into account sums over <a href="/wiki/Franck%E2%80%93Condon_principle" title="Franck–Condon principle">Franck-Condon's</a> active vibrational states and provides insight into electronic <a href="/wiki/Absorption_spectroscopy" title="Absorption spectroscopy">absorption</a> and <a href="/wiki/Emission_spectrum" title="Emission spectrum">emission spectra</a>. Nevertheless, the work highlights a flaw in the sum-over-states method, especially for large molecules like visible <a href="/wiki/Chromophore" title="Chromophore">chromophores</a>, which are commonly studied in Raman spectroscopy.<a href="#cite_note-:0-76">[76]</a> The difficulty arises from the potentially infinite number of intermediary steps needed. While lowering the sum at higher vibrational states can help tiny molecules get over this issue, larger molecules find it more challenging when there are more terms in the sum, particularly in the condensed phase when individual <a href="/wiki/Quantum_state" title="Quantum state">eigenstates</a> cannot be resolved spectrally.<a href="#cite_note-:0-76">[76]</a> To overcome this, two substitute techniques that do not require adding eigenstates can be considered. Among these two methods are available: the transform method.<a href="#cite_note-77">[77]</a><a href="#cite_note-78">[78]</a><a href="#cite_note-79">[79]</a> and Heller's time-dependent approach.<a href="#cite_note-80">[80]</a><a href="#cite_note-81">[81]</a><a href="#cite_note-82">[82]</a><a href="#cite_note-83">[83]</a> The goal of both approaches is to take into consideration the frequency-dependent Raman cross-section σR(ω0) of a particular normal mode.<a href="#cite_note-:0-76">[76]</a> <div class="mw-heading mw-heading2"><h2 id="Variants">Variants</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=16" title="Edit section: Variants">edit</a>]</div> At least 25 variations of Raman spectroscopy have been developed.<a href="#cite_note-Long-11">[11]</a> The usual purpose is to enhance the sensitivity (e.g., <a href="/wiki/Surface-enhanced_Raman_spectroscopy" title="Surface-enhanced Raman spectroscopy">Surface-enhanced Raman spectroscopy</a> (SERS)), to improve the spatial resolution (Raman microscopy), or to acquire very specific information (resonance Raman). <div class="mw-heading mw-heading3"><h3 id="Spontaneous_(or_far-field)_Raman_spectroscopy">Spontaneous (or far-field) Raman spectroscopy</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=17" title="Edit section: Spontaneous (or far-field) Raman spectroscopy">edit</a>]</div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:AFM_vs_Raman_imaging_of_GaSe.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/e/ed/AFM_vs_Raman_imaging_of_GaSe.jpg/220px-AFM_vs_Raman_imaging_of_GaSe.jpg" decoding="async" width="220" height="443" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/e/ed/AFM_vs_Raman_imaging_of_GaSe.jpg 1.5x" data-file-width="234" data-file-height="471" /></a><figcaption>Correlative Raman imaging: Comparison of topographical (<a href="/wiki/Atomic_force_microscopy" title="Atomic force microscopy">AFM</a>, top) and Raman images of <a href="/wiki/Gallium(II)_selenide" title="Gallium(II) selenide">GaSe</a>. Scale bar is 5 μm.<a href="#cite_note-84">[84]</a></figcaption></figure> Terms such as spontaneous Raman spectroscopy or normal Raman spectroscopy summarize Raman spectroscopy techniques based on Raman scattering by using normal <a href="/wiki/Near_and_far_field" title="Near and far field">far-field</a> optics as described above. Variants of normal Raman spectroscopy exist with respect to excitation-detection geometries, combination with other techniques, use of special (polarizing) optics and specific choice of excitation wavelengths for resonance enhancement. <ul><li><a href="/wiki/Raman_microscope#Correlative_Raman_imaging" title="Raman microscope">Correlative Raman imaging</a> – Raman microscopy can be combined with complementary imaging methods, such as <a href="/wiki/Atomic_force_microscopy" title="Atomic force microscopy">atomic force microscopy</a> (Raman-AFM) and <a href="/wiki/Scanning_electron_microscope" title="Scanning electron microscope">scanning electron microscopy</a> (Raman-SEM) to compare Raman distribution maps with (or overlay them onto) topographical or morphological images, and to correlate Raman spectra with complementary physical or chemical information (e.g., gained by SEM-<a href="/wiki/Energy-dispersive_X-ray_spectroscopy" title="Energy-dispersive X-ray spectroscopy">EDX</a>).</li> <li><a href="/wiki/Resonance_Raman_spectroscopy" title="Resonance Raman spectroscopy">Resonance Raman spectroscopy</a> – The excitation wavelength is matched to an electronic transition of the molecule or crystal, so that vibrational modes associated with the excited electronic state are greatly enhanced. This is useful for studying large molecules such as <a href="/wiki/Polypeptide" class="mw-redirect" title="Polypeptide">polypeptides</a>, which might show hundreds of bands in "conventional" Raman spectra. It is also useful for associating normal modes with their observed frequency shifts.<a href="#cite_note-85">[85]</a></li> <li>Angle-resolved Raman spectroscopy – Not only are standard Raman results recorded but also the angle with respect to the incident laser. If the orientation of the sample is known then detailed information about the phonon dispersion relation can also be gleaned from a single test.<a href="#cite_note-86">[86]</a></li> <li>Optical tweezers Raman spectroscopy (OTRS) – Used to study individual particles, and even biochemical processes in single cells trapped by <a href="/wiki/Optical_tweezers" title="Optical tweezers">optical tweezers</a>.<a href="#cite_note-87">[87]</a><a href="#cite_note-88">[88]</a><a href="#cite_note-89">[89]</a></li> <li><a href="/wiki/Spatially_offset_Raman_spectroscopy" title="Spatially offset Raman spectroscopy">Spatially offset Raman spectroscopy</a> (SORS) – The Raman scattering beneath an obscuring surface is retrieved from a scaled subtraction of two spectra taken at two spatially offset points.</li> <li><a href="/wiki/Raman_optical_activity" title="Raman optical activity">Raman optical activity</a> (ROA) – Measures vibrational optical activity by means of a small difference in the intensity of Raman scattering from chiral molecules in right- and left-circularly polarized incident light or, equivalently, a small circularly polarized component in the scattered light.<a href="#cite_note-90">[90]</a></li> <li><a href="/wiki/Transmission_raman" class="mw-redirect" title="Transmission raman">Transmission Raman</a> – Allows probing of a significant bulk of a <a href="/wiki/Turbid" class="mw-redirect" title="Turbid">turbid</a> material, such as powders, capsules, living tissue, etc. It was largely ignored following investigations in the late 1960s (<a href="/wiki/Bernhard_Schrader" title="Bernhard Schrader">Schrader</a> and Bergmann, 1967)<a href="#cite_note-91">[91]</a> but was rediscovered in 2006 as a means of rapid assay of <a href="/wiki/Pharmaceutical" class="mw-redirect" title="Pharmaceutical">pharmaceutical</a> <a href="/wiki/Dosage_forms" class="mw-redirect" title="Dosage forms">dosage forms</a>.<a href="#cite_note-92">[92]</a> There are medical diagnostic applications particularly in the detection of cancer.<a href="#cite_note-autogenerated1-37">[37]</a><a href="#cite_note-93">[93]</a><a href="#cite_note-94">[94]</a></li> <li>Micro-cavity substrates – A method that improves the detection limit of conventional Raman spectra using micro-Raman in a micro-cavity coated with reflective Au or Ag. The micro-cavity has a radius of several micrometers and enhances the entire Raman signal by providing multiple excitations of the sample and couples the forward-scattered Raman photons toward the collection optics in the back-scattered Raman geometry.<a href="#cite_note-95">[95]</a></li> <li>Stand-off remote Raman – In standoff Raman, the sample is measured at a distance from the Raman spectrometer, usually by using a telescope for light collection. Remote Raman spectroscopy was proposed in the 1960s<a href="#cite_note-96">[96]</a> and initially developed for the measurement of atmospheric gases.<a href="#cite_note-97">[97]</a> The technique was extended In 1992 by Angel et al. for standoff Raman detection of hazardous inorganic and organic compounds.<a href="#cite_note-98">[98]</a></li> <li><a href="/wiki/X-ray_Raman_scattering" title="X-ray Raman scattering">X-ray Raman scattering</a> – Measures electronic transitions rather than vibrations.<a href="#cite_note-99">[99]</a></li></ul> <div class="mw-heading mw-heading3"><h3 id="Enhanced_(or_near-field)_Raman_spectroscopy">Enhanced (or near-field) Raman spectroscopy</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=18" title="Edit section: Enhanced (or near-field) Raman spectroscopy">edit</a>]</div> Enhancement of Raman scattering is achieved by local electric-field enhancement by optical <a href="/wiki/Near_and_far_field" title="Near and far field">near-field</a> effects (e.g. localized <a href="/wiki/Surface_plasmon" title="Surface plasmon">surface plasmons</a>). <ul><li><a href="/wiki/Surface_Enhanced_Raman_Spectroscopy" class="mw-redirect" title="Surface Enhanced Raman Spectroscopy">Surface-enhanced Raman spectroscopy</a> (SERS) – Normally done in a silver or gold colloid or a substrate containing silver or gold. Surface <a href="/wiki/Plasmons" class="mw-redirect" title="Plasmons">plasmons</a> of silver and gold are excited by the laser, resulting in an increase in the electric fields surrounding the metal. Given that Raman intensities are proportional to the electric field, there is large increase in the measured signal (by up to 1011). This effect was originally observed by <a href="/wiki/Martin_Fleischmann" title="Martin Fleischmann">Martin Fleischmann</a> but the prevailing explanation was proposed by Van Duyne in 1977.<a href="#cite_note-100">[100]</a> A comprehensive theory of the effect was given by Lombardi and Birke.<a href="#cite_note-101">[101]</a></li> <li>Surface-enhanced resonance Raman spectroscopy (SERRS) – A combination of SERS and resonance Raman spectroscopy that uses proximity to a surface to increase Raman intensity, and excitation wavelength matched to the maximum absorbance of the molecule being analysed.</li> <li><a href="/wiki/Tip-enhanced_Raman_spectroscopy" title="Tip-enhanced Raman spectroscopy">Tip-enhanced Raman spectroscopy</a> (TERS) – TERS combines the chemical sensitivity of SERS with the high spatial resolution of scanning probe microscopy techniques, enabling chemical imaging of surfaces at the nanometre length-scale with high detection sensitivity.<a href="#cite_note-102">[102]</a> It uses a metallic (usually silver-/gold-coated AFM or STM) tip to enhance the Raman signals of molecules situated in its vicinity. The spatial resolution is approximately the size of the tip apex (20–30 nm). TERS has been shown to have sensitivity down to the single molecule level <a href="#cite_note-103">[103]</a><a href="#cite_note-104">[104]</a><a href="#cite_note-105">[105]</a><a href="#cite_note-106">[106]</a> and holds some promise for <a href="/wiki/Bioanalysis" title="Bioanalysis">bioanalysis</a> applications <a href="#cite_note-107">[107]</a> and DNA sequencing.<a href="#cite_note-He_753–757-68">[68]</a> TERS was used to image the vibrational normal modes of single molecules.<a href="#cite_note-108">[108]</a></li> <li><a href="/wiki/Surface_plasmon_polariton" title="Surface plasmon polariton">Surface plasmon polariton</a> enhanced Raman scattering (SPPERS) – This approach exploits apertureless metallic conical tips for near field excitation of molecules. This technique differs from the TERS approach due to its inherent capability of suppressing the background field. In fact, when an appropriate laser source impinges on the base of the cone, a TM0 mode<a href="#cite_note-109">[109]</a> (polaritonic mode) can be locally created, namely far away from the excitation spot (apex of the tip). The mode can propagate along the tip without producing any radiation field up to the tip apex where it interacts with the molecule. In this way, the focal plane is separated from the excitation plane by a distance given by the tip length, and no background plays any role in the Raman excitation of the molecule.<a href="#cite_note-110">[110]</a><a href="#cite_note-111">[111]</a><a href="#cite_note-112">[112]</a><a href="#cite_note-113">[113]</a></li></ul> <div class="mw-heading mw-heading3"><h3 id="Non-linear_Raman_spectroscopy">Non-linear Raman spectroscopy</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=19" title="Edit section: Non-linear Raman spectroscopy">edit</a>]</div> Raman signal enhancements are achieved through non-linear optical effects, typically realized by mixing two or more wavelengths emitted by spatially and temporally synchronized pulsed lasers. <ul><li>Hyper Raman – A <a href="/wiki/Non-linear_optics" class="mw-redirect" title="Non-linear optics">non-linear</a> effect in which the vibrational modes interact with the <a href="/wiki/Second_harmonic_generation" class="mw-redirect" title="Second harmonic generation">second harmonic</a> of the excitation beam. This requires very high power, but allows the observation of vibrational modes that are normally "silent". It frequently relies on SERS-type enhancement to boost the sensitivity.<a href="#cite_note-114">[114]</a></li> <li><a href="/wiki/Stimulated_Raman_spectroscopy" title="Stimulated Raman spectroscopy">Stimulated Raman spectroscopy</a> (SRS) – A <a href="/wiki/Pump-probe_spectroscopy" class="mw-redirect" title="Pump-probe spectroscopy">pump-probe</a> technique, where a spatially coincident, two color pulse (with polarization either parallel or perpendicular) transfers the population from ground to a <a href="/wiki/Rotational%E2%80%93vibrational_coupling" title="Rotational–vibrational coupling">rovibrationally</a> excited state. If the difference in energy corresponds to an allowed Raman transition, scattered light will correspond to loss or gain in the pump beam.</li> <li><a href="/wiki/Inverse_Raman_effect" class="mw-redirect" title="Inverse Raman effect">Inverse Raman spectroscopy</a> – A synonym for stimulated Raman loss spectroscopy.</li> <li><a href="/wiki/Coherent_anti-Stokes_Raman_spectroscopy" title="Coherent anti-Stokes Raman spectroscopy">Coherent anti-Stokes Raman spectroscopy</a> (CARS) – Two laser beams are used to generate a coherent anti-Stokes frequency beam, which can be enhanced by resonance.</li></ul> <div class="mw-heading mw-heading3"><h3 id="Morphologically-Directed_Raman_spectroscopy">Morphologically-Directed Raman spectroscopy</h3>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=20" title="Edit section: Morphologically-Directed Raman spectroscopy">edit</a>]</div> Morphologically Directed Raman Spectroscopy (MDRS) combines automated particle imaging and Raman microspectroscopy into a singular integrated platform in order to provide particle size, shape, and chemical identification.<a href="#cite_note-MalvernMDRS-115">[115]</a><a href="#cite_note-QualitycontrolMDRS-116">[116]</a><a href="#cite_note-SpectroscopyOnlineMDRS-117">[117]</a> Automated particle imaging determines the particle size and shape distributions of components within a blended sample from images of individual particles.<a href="#cite_note-QualitycontrolMDRS-116">[116]</a><a href="#cite_note-SpectroscopyOnlineMDRS-117">[117]</a> The information gathered from automated particle imaging is then utilized to direct the Raman spectroscopic analysis.<a href="#cite_note-MalvernMDRS-115">[115]</a> The Raman spectroscopic analytical process is performed on a randomly-selected subset of the particles, allowing chemical identification of the sample’s multiple components.<a href="#cite_note-MalvernMDRS-115">[115]</a> Tens of thousands of particles can be imaged in a matter of minutes using the MDRS method, making the process ideal for forensic analysis and investigating counterfeit pharmaceuticals and subsequent adjudications.<a href="#cite_note-QualitycontrolMDRS-116">[116]</a><a href="#cite_note-SpectroscopyOnlineMDRS-117">[117]</a> <div class="mw-heading mw-heading2"><h2 id="References">References</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=21" title="Edit section: References">edit</a>]</div> <style data-mw-deduplicate="TemplateStyles:r1239543626">.mw-parser-output .reflist{margin-bottom:0.5em;list-style-type:decimal}@media screen{.mw-parser-output .reflist{font-size:90%}}.mw-parser-output .reflist .references{font-size:100%;margin-bottom:0;list-style-type:inherit}.mw-parser-output .reflist-columns-2{column-width:30em}.mw-parser-output .reflist-columns-3{column-width:25em}.mw-parser-output .reflist-columns{margin-top:0.3em}.mw-parser-output .reflist-columns ol{margin-top:0}.mw-parser-output .reflist-columns li{page-break-inside:avoid;break-inside:avoid-column}.mw-parser-output .reflist-upper-alpha{list-style-type:upper-alpha}.mw-parser-output .reflist-upper-roman{list-style-type:upper-roman}.mw-parser-output .reflist-lower-alpha{list-style-type:lower-alpha}.mw-parser-output .reflist-lower-greek{list-style-type:lower-greek}.mw-parser-output .reflist-lower-roman{list-style-type:lower-roman}</style><div class="reflist reflist-columns references-column-width" style="column-width: 30em;"> <ol class="references"> <li id="cite_note-Gardiner-1">^ <a href="#cite_ref-Gardiner_1-0">a</a> <a href="#cite_ref-Gardiner_1-1">b</a> <style data-mw-deduplicate="TemplateStyles:r1238218222">.mw-parser-output cite.citation{font-style:inherit;word-wrap:break-word}.mw-parser-output .citation q{quotes:"\"""\"""'""'"}.mw-parser-output .citation:target{background-color:rgba(0,127,255,0.133)}.mw-parser-output .id-lock-free.id-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/6/65/Lock-green.svg")right 0.1em center/9px no-repeat}.mw-parser-output .id-lock-limited.id-lock-limited a,.mw-parser-output .id-lock-registration.id-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/d/d6/Lock-gray-alt-2.svg")right 0.1em center/9px no-repeat}.mw-parser-output .id-lock-subscription.id-lock-subscription a{background:url("//upload.wikimedia.org/wikipedia/commons/a/aa/Lock-red-alt-2.svg")right 0.1em center/9px no-repeat}.mw-parser-output .cs1-ws-icon a{background:url("//upload.wikimedia.org/wikipedia/commons/4/4c/Wikisource-logo.svg")right 0.1em center/12px no-repeat}body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-free a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-limited a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-registration a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-subscription a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .cs1-ws-icon a{background-size:contain;padding:0 1em 0 0}.mw-parser-output .cs1-code{color:inherit;background:inherit;border:none;padding:inherit}.mw-parser-output .cs1-hidden-error{display:none;color:var(--color-error,#d33)}.mw-parser-output .cs1-visible-error{color:var(--color-error,#d33)}.mw-parser-output .cs1-maint{display:none;color:#085;margin-left:0.3em}.mw-parser-output .cs1-kern-left{padding-left:0.2em}.mw-parser-output .cs1-kern-right{padding-right:0.2em}.mw-parser-output .citation .mw-selflink{font-weight:inherit}@media screen{.mw-parser-output .cs1-format{font-size:95%}html.skin-theme-clientpref-night .mw-parser-output .cs1-maint{color:#18911f}}@media screen and (prefers-color-scheme:dark){html.skin-theme-clientpref-os .mw-parser-output .cs1-maint{color:#18911f}}</style><cite id="CITEREFGardiner1989" class="citation book cs1">Gardiner, D.J. (1989). Practical Raman spectroscopy. <a href="/wiki/Springer-Verlag" class="mw-redirect" title="Springer-Verlag">Springer-Verlag</a>. <a href="/wiki/ISBN_(identifier)" class="mw-redirect" title="ISBN (identifier)">ISBN</a> <a href="/wiki/Special:BookSources/978-0-387-50254-0" title="Special:BookSources/978-0-387-50254-0"><bdi>978-0-387-50254-0</bdi></a>.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=Practical+Raman+spectroscopy&rft.pub=Springer-Verlag&rft.date=1989&rft.isbn=978-0-387-50254-0&rft.aulast=Gardiner&rft.aufirst=D.J.&rfr_id=info%3Asid%2Fen.wikipedia.org%3ARaman+spectroscopy" class="Z3988"> </li> <li id="cite_note-2"><a href="#cite_ref-2">^</a> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite id="CITEREFHollas1996" class="citation book cs1">Hollas, J. Michael (1996). "6". Modern Spectroscopy (3rd ed.). John Wiley and Sons. p. 139. <a href="/wiki/ISBN_(identifier)" class="mw-redirect" title="ISBN (identifier)">ISBN</a> <a href="/wiki/Special:BookSources/0-471-96523-5" title="Special:BookSources/0-471-96523-5"><bdi>0-471-96523-5</bdi></a>. <q>The use of vibrational Raman spectroscopy in qualitative analysis has increased greatly since the introduction of lasers ... as monochromatic sources.</q></cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=bookitem&rft.atitle=6&rft.btitle=Modern+Spectroscopy&rft.pages=139&rft.edition=3rd&rft.pub=John+Wiley+and+Sons&rft.date=1996&rft.isbn=0-471-96523-5&rft.aulast=Hollas&rft.aufirst=J.+Michael&rfr_id=info%3Asid%2Fen.wikipedia.org%3ARaman+spectroscopy" class="Z3988"> </li> <li id="cite_note-3"><a href="#cite_ref-3">^</a> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite id="CITEREFSmekal1923" class="citation journal cs1">Smekal, A. (1923). "Zur Quantentheorie der Dispersion". Die Naturwissenschaften. 11 (43): 873–875. <a href="/wiki/Bibcode_(identifier)" class="mw-redirect" title="Bibcode (identifier)">Bibcode</a>:<a rel="nofollow" class="external text" href="https://ui.adsabs.harvard.edu/abs/1923NW.....11..873S">1923NW.....11..873S</a>. <a href="/wiki/Doi_(identifier)" class="mw-redirect" title="Doi (identifier)">doi</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1007%2FBF01576902">10.1007/BF01576902</a>. <a href="/wiki/S2CID_(identifier)" class="mw-redirect" title="S2CID (identifier)">S2CID</a> <a rel="nofollow" class="external text" href="https://api.semanticscholar.org/CorpusID:20086350">20086350</a>.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.jtitle=Die+Naturwissenschaften&rft.atitle=Zur+Quantentheorie+der+Dispersion&rft.volume=11&rft.issue=43&rft.pages=873-875&rft.date=1923&rft_id=https%3A%2F%2Fapi.semanticscholar.org%2FCorpusID%3A20086350%23id-name%3DS2CID&rft_id=info%3Adoi%2F10.1007%2FBF01576902&rft_id=info%3Abibcode%2F1923NW.....11..873S&rft.aulast=Smekal&rft.aufirst=A.&rfr_id=info%3Asid%2Fen.wikipedia.org%3ARaman+spectroscopy" class="Z3988"> </li> <li id="cite_note-4"><a href="#cite_ref-4">^</a> <a rel="nofollow" class="external text" href="http://oralhistories.library.caltech.edu/archive/00000070/01/OH_Rasetti.pdf">Caltech oral history interview</a> by <a href="/wiki/Judith_R._Goodstein" title="Judith R. Goodstein">Judith R. Goodstein</a>, 4 February 1982 </li> <li id="cite_note-5"><a href="#cite_ref-5">^</a> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite id="CITEREFBattimelli,_Giovanni2002" class="citation journal cs1">Battimelli, Giovanni (December 2002). <a rel="nofollow" class="external text" href="https://doi.org/10.1063%2F1.1537927">"Obituary: Franco Rasetti"</a>. 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Quality Control. Manufacturing Chemist. October 2016.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.jtitle=Quality+Control&rft.atitle=Introducing+morphologically+directed+Raman+spectroscopy%3A+A+powerful+tool+for+the+detection+of+counterfeit+drugs&rft.date=2016-10&rfr_id=info%3Asid%2Fen.wikipedia.org%3ARaman+spectroscopy" class="Z3988"> </li> <li id="cite_note-SpectroscopyOnlineMDRS-117">^ <a href="#cite_ref-SpectroscopyOnlineMDRS_117-0">a</a> <a href="#cite_ref-SpectroscopyOnlineMDRS_117-1">b</a> <a href="#cite_ref-SpectroscopyOnlineMDRS_117-2">c</a> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite class="citation news cs1"><a rel="nofollow" class="external text" href="https://core.ac.uk/download/pdf/214330323.pdf">"Morphologically Directed Raman Spectroscopic Analysis of Forensic Samples"</a> (PDF). Spectroscopy Onlinet. January 2018.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.jtitle=Spectroscopy+Onlinet&rft.atitle=Morphologically+Directed+Raman+Spectroscopic+Analysis+of+Forensic+Samples&rft.date=2018-01&rft_id=https%3A%2F%2Fcore.ac.uk%2Fdownload%2Fpdf%2F214330323.pdf&rfr_id=info%3Asid%2Fen.wikipedia.org%3ARaman+spectroscopy" class="Z3988"> </li> </ol></div> <div class="mw-heading mw-heading2"><h2 id="Further_reading">Further reading</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=22" title="Edit section: Further reading">edit</a>]</div> <ul><li><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite id="CITEREFVandenabeele2013" class="citation book cs1">Vandenabeele, Peter (2013). <a rel="nofollow" class="external text" href="https://web.archive.org/web/20190427213735/https://www.wiley.com/en-se/Practical%2BRaman%2BSpectroscopy:%2BAn%2BIntroduction-p-9780470683194">Practical Raman spectroscopy: An Introduction</a>. Wiley. <a href="/wiki/ISBN_(identifier)" class="mw-redirect" title="ISBN (identifier)">ISBN</a> <a href="/wiki/Special:BookSources/978-0470683194" title="Special:BookSources/978-0470683194"><bdi>978-0470683194</bdi></a>. Archived from <a rel="nofollow" class="external text" href="https://www.wiley.com/en-se/Practical+Raman+Spectroscopy:+An+Introduction-p-9780470683194">the original</a> on 2019-04-27. Retrieved 2019-04-27.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=Practical+Raman+spectroscopy%3A+An+Introduction&rft.pub=Wiley&rft.date=2013&rft.isbn=978-0470683194&rft.aulast=Vandenabeele&rft.aufirst=Peter&rft_id=https%3A%2F%2Fwww.wiley.com%2Fen-se%2FPractical%2BRaman%2BSpectroscopy%3A%2BAn%2BIntroduction-p-9780470683194&rfr_id=info%3Asid%2Fen.wikipedia.org%3ARaman+spectroscopy" class="Z3988"></li></ul> <div class="mw-heading mw-heading2"><h2 id="External_links">External links</h2>[<a href="/w/index.php?title=Raman_spectroscopy&action=edit&section=23" title="Edit section: External links">edit</a>]</div> <style data-mw-deduplicate="TemplateStyles:r1235681985">.mw-parser-output .side-box{margin:4px 0;box-sizing:border-box;border:1px solid #aaa;font-size:88%;line-height:1.25em;background-color:var(--background-color-interactive-subtle,#f8f9fa);display:flow-root}.mw-parser-output .side-box-abovebelow,.mw-parser-output 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href="/wiki/Template:Raman_spectroscopy" title="Template:Raman spectroscopy"><abbr title="View this template">v</abbr></a></li><li class="nv-talk"><a href="/wiki/Template_talk:Raman_spectroscopy" title="Template talk:Raman spectroscopy"><abbr title="Discuss this template">t</abbr></a></li><li class="nv-edit"><a href="/wiki/Special:EditPage/Template:Raman_spectroscopy" title="Special:EditPage/Template:Raman spectroscopy"><abbr title="Edit this template">e</abbr></a></li></ul></div><div id="Raman_spectroscopy" style="font-size:114%;margin:0 4em"><a class="mw-selflink selflink">Raman spectroscopy</a></div></th></tr><tr><th scope="row" class="navbox-group" style="width:1%;text-align:center">Techniques</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Coherent_anti-Stokes_Raman_spectroscopy" title="Coherent anti-Stokes Raman spectroscopy">Coherent anti-Stokes Raman spectroscopy</a></li> <li><a href="/wiki/Raman_optical_activity" title="Raman optical activity">Raman optical activity</a></li> <li><a href="/wiki/Resonance_Raman_spectroscopy" title="Resonance Raman spectroscopy">Resonance Raman spectroscopy</a></li> <li><a href="/wiki/Rotating-polarization_coherent_anti-Stokes_Raman_spectroscopy" title="Rotating-polarization coherent anti-Stokes Raman spectroscopy">Rotating-polarization coherent anti-Stokes Raman spectroscopy</a></li> <li><a href="/wiki/Spatially_offset_Raman_spectroscopy" title="Spatially offset Raman spectroscopy">Spatially offset Raman spectroscopy</a></li> <li><a href="/wiki/Stimulated_Raman_spectroscopy" title="Stimulated Raman spectroscopy">Stimulated Raman spectroscopy</a></li> <li><a href="/wiki/Surface-enhanced_Raman_spectroscopy" title="Surface-enhanced Raman spectroscopy">Surface-enhanced Raman spectroscopy</a></li> <li><a href="/wiki/Tip-enhanced_Raman_spectroscopy" title="Tip-enhanced Raman spectroscopy">Tip-enhanced Raman spectroscopy</a></li> <li><a href="/wiki/Transmission_Raman_spectroscopy" title="Transmission Raman spectroscopy">Transmission Raman spectroscopy</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%;text-align:center">Applications</th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Raman_amplification" title="Raman amplification">Raman amplification</a></li> <li><a href="/wiki/Raman_cooling" title="Raman cooling">Raman cooling</a></li> <li><a href="/wiki/Raman_laser" title="Raman laser">Raman laser</a></li> <li><a href="/wiki/Raman_microscope" title="Raman microscope">Raman microscope</a></li> <li><a href="/wiki/SHERLOC" class="mw-redirect" title="SHERLOC">SHERLOC</a></li> <li><a href="/wiki/Stimulated_Raman_adiabatic_passage" title="Stimulated Raman adiabatic passage">Stimulated Raman adiabatic passage</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%;text-align:center">Theory</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Depolarization_ratio" title="Depolarization ratio">Depolarization ratio</a></li> <li><a href="/wiki/Four-wave_mixing" title="Four-wave mixing">Four-wave mixing</a></li> <li><a href="/wiki/Nonlinear_optics" title="Nonlinear optics">Nonlinear optics</a></li> <li><a href="/wiki/Raman_scattering" title="Raman scattering">Raman scattering</a></li> <li><a href="/wiki/Rayleigh_scattering" title="Rayleigh scattering">Rayleigh scattering</a></li> <li><a href="/wiki/Rule_of_mutual_exclusion" title="Rule of mutual exclusion">Rule of mutual exclusion</a></li> <li><a href="/wiki/Stokes_shift" title="Stokes shift">Stokes shift</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%;text-align:center">Journals</th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Journal_of_Raman_Spectroscopy" title="Journal of Raman Spectroscopy">Journal of Raman Spectroscopy</a></li> <li><a href="/wiki/Vibrational_Spectroscopy" title="Vibrational Spectroscopy">Vibrational Spectroscopy</a></li></ul> </div></td></tr><tr><td class="navbox-abovebelow" colspan="2"><div> <ul><li><img alt="" src="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/16px-Symbol_category_class.svg.png" decoding="async" width="16" height="16" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/23px-Symbol_category_class.svg.png 1.5x, //upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/31px-Symbol_category_class.svg.png 2x" data-file-width="180" data-file-height="185" /><a href="/wiki/Category:Raman_spectroscopy" title="Category:Raman spectroscopy">Category</a></li> <li><img alt="" 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mw-collapsible autocollapse navbox-inner" style="border-spacing:0;background:transparent;color:inherit"><tbody><tr><th scope="col" class="navbox-title" colspan="2"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1129693374"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1239400231"><div class="navbar plainlinks hlist navbar-mini"><ul><li class="nv-view"><a href="/wiki/Template:Branches_of_spectroscopy" title="Template:Branches of spectroscopy"><abbr title="View this template">v</abbr></a></li><li class="nv-talk"><a href="/wiki/Template_talk:Branches_of_spectroscopy" title="Template talk:Branches of spectroscopy"><abbr title="Discuss this template">t</abbr></a></li><li class="nv-edit"><a href="/wiki/Special:EditPage/Template:Branches_of_spectroscopy" title="Special:EditPage/Template:Branches of spectroscopy"><abbr title="Edit this template">e</abbr></a></li></ul></div><div id="Spectroscopy" style="font-size:114%;margin:0 4em"><a href="/wiki/Spectroscopy" title="Spectroscopy">Spectroscopy</a></div></th></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Infrared_spectroscopy" title="Infrared spectroscopy">Vibrational (IR)</a></th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Fourier-transform_infrared_spectroscopy" title="Fourier-transform infrared spectroscopy">FT-IR</a></li> <li><a class="mw-selflink selflink">Raman</a></li> <li><a href="/wiki/Resonance_Raman_spectroscopy" title="Resonance Raman spectroscopy">Resonance Raman</a></li> <li><a href="/wiki/Rotational_spectroscopy" title="Rotational spectroscopy">Rotational</a></li> <li><a href="/wiki/Rotational%E2%80%93vibrational_spectroscopy" title="Rotational–vibrational spectroscopy">Rotational–vibrational</a></li> <li><a href="/wiki/Molecular_vibration" title="Molecular vibration">Vibrational</a></li> <li><a href="/wiki/Vibrational_circular_dichroism" title="Vibrational circular dichroism">Vibrational circular dichroism</a></li> <li><a href="/wiki/Nuclear_resonance_vibrational_spectroscopy" title="Nuclear resonance vibrational spectroscopy">Nuclear resonance vibrational spectroscopy</a></li> <li><a href="/wiki/Vibrational_spectroscopy_of_linear_molecules" title="Vibrational spectroscopy of linear molecules">Vibrational spectroscopy of linear molecules</a></li> <li><a href="/wiki/Thermal_infrared_spectroscopy" title="Thermal infrared spectroscopy">Thermal infrared spectroscopy</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">UV–Vis–NIR "Optical"</th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Ultraviolet%E2%80%93visible_spectroscopy" title="Ultraviolet–visible spectroscopy">Ultraviolet–visible</a></li> <li><a href="/wiki/Fluorescence_spectroscopy" title="Fluorescence spectroscopy">Fluorescence</a> <ul><li><a href="/wiki/Cold_vapour_atomic_fluorescence_spectroscopy" title="Cold vapour atomic fluorescence spectroscopy">Cold vapour atomic</a></li></ul></li> <li><a href="/wiki/Vibronic_spectroscopy" title="Vibronic spectroscopy">Vibronic</a></li> <li><a href="/wiki/Near-infrared_spectroscopy" title="Near-infrared spectroscopy">Near-infrared</a></li> <li><a href="/wiki/Resonance-enhanced_multiphoton_ionization" title="Resonance-enhanced multiphoton ionization">Resonance-enhanced multiphoton ionization</a> (REMPI)</li> <li><a class="mw-selflink selflink">Raman</a> <ul><li><a href="/wiki/Coherent_anti-Stokes_Raman_spectroscopy" title="Coherent anti-Stokes Raman spectroscopy">Coherent anti-Stokes</a></li></ul></li> <li><a href="/wiki/Raman_optical_activity" title="Raman optical activity">Raman optical activity</a></li> <li><a href="/wiki/Laser-induced_breakdown_spectroscopy" title="Laser-induced breakdown spectroscopy">Laser-induced breakdown</a></li> <li><a href="/wiki/Atomic_spectroscopy" title="Atomic spectroscopy">Atomic</a> <ul><li><a href="/wiki/Atomic_emission_spectroscopy" title="Atomic emission spectroscopy">emission</a> <ul><li><a href="/wiki/Glow-discharge_optical_emission_spectroscopy" title="Glow-discharge optical emission spectroscopy">Glow-discharge optical</a></li></ul></li> <li><a href="/wiki/Atomic_absorption_spectroscopy" title="Atomic absorption spectroscopy">absorption</a></li></ul></li> <li><a href="/wiki/Cavity_ring-down_spectroscopy" title="Cavity ring-down spectroscopy">Cavity ring-down spectroscopy</a></li> <li><a href="/wiki/Saturated_absorption_spectroscopy" title="Saturated absorption spectroscopy">Saturated absorption spectroscopy</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">X-ray and Gamma ray</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/X-ray_spectroscopy" title="X-ray spectroscopy">X-ray</a> <ul><li><a href="/wiki/Energy-dispersive_X-ray_spectroscopy" title="Energy-dispersive X-ray spectroscopy">Energy-dispersive</a></li> <li><a href="/wiki/X-ray_emission_spectroscopy" title="X-ray emission spectroscopy">Emission</a></li> <li><a href="/wiki/Extended_X-ray_absorption_fine_structure" title="Extended X-ray absorption fine structure">Extended X-ray absorption fine structure</a></li></ul></li> <li><a href="/wiki/Gamma_spectroscopy" title="Gamma spectroscopy">Gamma</a></li> <li><a href="/wiki/M%C3%B6ssbauer_spectroscopy" title="Mössbauer spectroscopy">Mössbauer</a> <ul><li><a href="/wiki/Conversion_electron_M%C3%B6ssbauer_spectroscopy" title="Conversion electron Mössbauer spectroscopy">Conversion electron</a></li></ul></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Electron</th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Photoemission_spectroscopy" title="Photoemission spectroscopy">Photoelectron/photoemission</a> <ul><li><a href="/wiki/X-ray_photoelectron_spectroscopy" title="X-ray photoelectron spectroscopy">X-ray</a></li> <li><a href="/wiki/Ultraviolet_photoelectron_spectroscopy" title="Ultraviolet photoelectron spectroscopy">UV</a></li> <li><a href="/wiki/Angle-resolved_photoemission_spectroscopy" title="Angle-resolved photoemission spectroscopy">Angle-resolved</a></li> <li><a href="/wiki/Two-photon_photoelectron_spectroscopy" title="Two-photon photoelectron spectroscopy">Two-photon</a></li></ul></li> <li><a href="/wiki/Auger_electron_spectroscopy" title="Auger electron spectroscopy">Auger</a></li> <li><a href="/wiki/Electron_phenomenological_spectroscopy" title="Electron phenomenological spectroscopy">phenomenological</a></li> <li><a href="/wiki/Electron_paramagnetic_resonance" title="Electron paramagnetic resonance">paramagnetic</a></li> <li><a href="/wiki/Beta_spectroscopy" class="mw-redirect" title="Beta spectroscopy">Beta spectroscopy</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Nucleon</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Alpha-particle_spectroscopy" title="Alpha-particle spectroscopy">Alpha</a></li> <li><a href="/wiki/Inelastic_neutron_scattering" class="mw-redirect" title="Inelastic neutron scattering">Inelastic neutron scattering</a> <ul><li><a href="/wiki/Neutron_spin_echo" title="Neutron spin echo">Neutron spin echo</a></li></ul></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Radiowave</th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Nuclear_magnetic_resonance_spectroscopy" title="Nuclear magnetic resonance spectroscopy">NMR</a> <ul><li><a href="/wiki/Two-dimensional_nuclear_magnetic_resonance_spectroscopy" title="Two-dimensional nuclear magnetic resonance spectroscopy">2D</a></li></ul></li> <li><a href="/wiki/Terahertz_spectroscopy_and_technology" title="Terahertz spectroscopy and technology">Terahertz</a></li> <li><a href="/wiki/Electron_paramagnetic_resonance" title="Electron paramagnetic resonance">ESR/EPR</a></li> <li><a href="/wiki/Ferromagnetic_resonance" title="Ferromagnetic resonance">Ferromagnetic resonance</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Others</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"></div><table class="nowraplinks hlist navbox-subgroup" style="border-spacing:0"><tbody><tr><th scope="row" class="navbox-group" style="width:1%">Data collection, processing</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Fourier-transform_spectroscopy" title="Fourier-transform spectroscopy">Fourier-transform spectroscopy</a></li> <li><a href="/wiki/Hyperspectral_imaging" title="Hyperspectral imaging">Hyperspectral imaging</a></li> <li><a href="/wiki/Spectrophotometry" title="Spectrophotometry">Spectrophotometry</a></li> <li><a href="/wiki/Time_stretch_analog-to-digital_converter" class="mw-redirect" title="Time stretch analog-to-digital converter">Time-stretch</a></li> <li><a href="/wiki/Time-resolved_spectroscopy" title="Time-resolved spectroscopy">Time-resolved spectroscopy</a></li> <li><a href="/wiki/Video_spectroscopy" title="Video spectroscopy">Video spectroscopy</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Measured phenomena</th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Acoustic_resonance_spectroscopy" title="Acoustic resonance spectroscopy">Acoustic resonance spectroscopy</a></li> <li><a href="/wiki/Circular_dichroism" title="Circular dichroism">Circular dichroism spectroscopy</a></li> <li><a href="/wiki/Deep-level_transient_spectroscopy" title="Deep-level transient spectroscopy">Deep-level transient spectroscopy</a></li> <li><a href="/wiki/Dual-polarization_interferometry" title="Dual-polarization interferometry">Dual-polarization interferometry</a></li> <li><a href="/wiki/Hadron_spectroscopy" title="Hadron spectroscopy">Hadron spectroscopy</a></li> <li><a href="/wiki/Inelastic_electron_tunneling_spectroscopy" title="Inelastic electron tunneling spectroscopy">Inelastic electron tunneling spectroscopy</a> <ul><li><a href="/wiki/Scanning_tunneling_spectroscopy" title="Scanning tunneling spectroscopy">Scanning tunneling spectroscopy</a></li></ul></li> <li><a href="/wiki/Photoacoustic_spectroscopy" title="Photoacoustic spectroscopy">Photoacoustic spectroscopy</a></li> <li><a href="/wiki/Photothermal_spectroscopy" title="Photothermal spectroscopy">Photothermal spectroscopy</a></li> <li><a href="/wiki/Pump%E2%80%93probe_spectroscopy" class="mw-redirect" title="Pump–probe spectroscopy">Pump–probe spectroscopy</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Applications</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Astronomical_spectroscopy" title="Astronomical spectroscopy">Astronomical spectroscopy</a></li> <li><a href="/wiki/Force_spectroscopy" title="Force spectroscopy">Force spectroscopy</a> (a misnomer)</li></ul> </div></td></tr></tbody></table><div></div></td></tr><tr><td class="navbox-abovebelow" colspan="2"><div> <ul><li><img alt="" src="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/16px-Symbol_category_class.svg.png" decoding="async" width="16" height="16" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/23px-Symbol_category_class.svg.png 1.5x, //upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/31px-Symbol_category_class.svg.png 2x" data-file-width="180" data-file-height="185" /> <a href="/wiki/Category:Spectroscopy" title="Category:Spectroscopy">Category</a></li> <li><img alt="" src="//upload.wikimedia.org/wikipedia/en/thumb/4/4a/Commons-logo.svg/12px-Commons-logo.svg.png" decoding="async" width="12" height="16" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/en/thumb/4/4a/Commons-logo.svg/18px-Commons-logo.svg.png 1.5x, //upload.wikimedia.org/wikipedia/en/thumb/4/4a/Commons-logo.svg/24px-Commons-logo.svg.png 2x" data-file-width="1024" data-file-height="1376" /> <a href="https://commons.wikimedia.org/wiki/Category:Spectroscopy" class="extiw" title="commons:Category:Spectroscopy">Commons</a></li></ul> </div></td></tr></tbody></table></div> <div class="navbox-styles"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1129693374"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1236075235"></div><div role="navigation" class="navbox" aria-labelledby="Lasers" style="padding:3px"><table class="nowraplinks hlist mw-collapsible mw-collapsed navbox-inner" style="border-spacing:0;background:transparent;color:inherit"><tbody><tr><th scope="col" class="navbox-title" colspan="2"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1129693374"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1239400231"><div class="navbar plainlinks hlist navbar-mini"><ul><li class="nv-view"><a href="/wiki/Template:Lasers" title="Template:Lasers"><abbr title="View this template">v</abbr></a></li><li class="nv-talk"><a href="/wiki/Template_talk:Lasers" title="Template talk:Lasers"><abbr title="Discuss this template">t</abbr></a></li><li class="nv-edit"><a href="/wiki/Special:EditPage/Template:Lasers" title="Special:EditPage/Template:Lasers"><abbr title="Edit this template">e</abbr></a></li></ul></div><div id="Lasers" style="font-size:114%;margin:0 4em"><a href="/wiki/Laser" title="Laser">Lasers</a></div></th></tr><tr><td class="navbox-abovebelow" colspan="2"><div> <ul><li><a href="/wiki/List_of_laser_articles" title="List of laser articles">List of laser articles</a></li> <li><a href="/wiki/List_of_laser_types" title="List of laser types">List of laser types</a></li> <li><a href="/wiki/List_of_laser_applications" title="List of laser applications">List of laser applications</a></li> <li><a href="/wiki/Laser_acronyms" title="Laser acronyms">Laser acronyms</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Types of lasers</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Chemical_laser" title="Chemical laser">Chemical laser</a></li> <li><a href="/wiki/Dye_laser" title="Dye laser">Dye laser</a> <ul><li><a href="/wiki/Bubble_laser" title="Bubble laser">Bubble</a></li> <li><a href="/wiki/Liquid-crystal_laser" title="Liquid-crystal laser">Liquid-crystal</a></li></ul></li> <li><a href="/wiki/Gas_laser" title="Gas laser">Gas laser</a> <ul><li><a href="/wiki/Carbon_dioxide_laser" class="mw-redirect" title="Carbon dioxide laser">Carbon dioxide</a></li> <li><a href="/wiki/Excimer_laser" title="Excimer laser">Excimer</a></li> <li><a href="/wiki/Helium%E2%80%93neon_laser" title="Helium–neon laser">Helium–neon</a></li> <li><a href="/wiki/Ion_laser" title="Ion laser">Ion</a></li> <li><a href="/wiki/Nitrogen_laser" title="Nitrogen laser">Nitrogen</a></li></ul></li> <li><a href="/wiki/Free-electron_laser" title="Free-electron laser">Free-electron laser</a></li> <li><a href="/wiki/Laser_diode" title="Laser diode">Laser diode</a></li> <li><a href="/wiki/Solid-state_laser" title="Solid-state laser">Solid-state laser</a> <ul><li><a href="/wiki/Er:YAG_laser" title="Er:YAG laser">Er:YAG</a></li> <li><a href="/wiki/Nd:YAG_laser" title="Nd:YAG laser">Nd:YAG</a></li> <li><a href="/wiki/Raman_laser" title="Raman laser">Raman</a></li> <li><a href="/wiki/Ruby_laser" title="Ruby laser">Ruby</a></li> <li><a href="/wiki/Ti-sapphire_laser" class="mw-redirect" title="Ti-sapphire laser">Ti-sapphire</a></li></ul></li> <li><a href="/wiki/X-ray_laser" title="X-ray laser">X-ray laser</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Laser_science" title="Laser science">Laser physics</a></th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Active_laser_medium" title="Active laser medium">Active laser medium</a></li> <li><a href="/wiki/Amplified_spontaneous_emission" title="Amplified spontaneous emission">Amplified spontaneous emission</a></li> <li><a href="/wiki/Continuous_wave" title="Continuous wave">Continuous wave</a></li> <li><a href="/wiki/Laser_ablation" title="Laser ablation">Laser ablation</a></li> <li><a href="/wiki/Laser_linewidth" title="Laser linewidth">Laser linewidth</a></li> <li><a href="/wiki/Lasing_threshold" title="Lasing threshold">Lasing threshold</a></li> <li><a href="/wiki/Population_inversion" title="Population inversion">Population inversion</a></li> <li><a href="/wiki/Ultrashort_pulse_laser" title="Ultrashort pulse laser">Ultrashort pulse</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Laser optics</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Beam_expander" title="Beam expander">Beam expander</a></li> <li><a href="/wiki/Beam_homogenizer" title="Beam homogenizer">Beam homogenizer</a></li> <li><a href="/wiki/Chirped_pulse_amplification" title="Chirped pulse amplification">Chirped pulse amplification</a></li> <li><a href="/wiki/Gain-switching" title="Gain-switching">Gain-switching</a></li> <li><a href="/wiki/Gaussian_beam" title="Gaussian beam">Gaussian beam</a></li> <li><a href="/wiki/Injection_seeder" title="Injection seeder">Injection seeder</a></li> <li><a href="/wiki/Laser_beam_profiler" title="Laser beam profiler">Laser beam profiler</a></li> <li><a href="/wiki/M_squared" title="M squared">M squared</a></li> <li><a href="/wiki/Mode_locking" title="Mode locking">Mode locking</a></li> <li><a href="/wiki/Multiple-prism_grating_laser_oscillator" title="Multiple-prism grating laser oscillator">Multiple-prism grating laser oscillator</a></li> <li><a href="/wiki/Optical_amplifier" title="Optical amplifier">Optical amplifier</a></li> <li><a href="/wiki/Optical_cavity" title="Optical cavity">Optical cavity</a></li> <li><a href="/wiki/Optical_isolator" title="Optical isolator">Optical isolator</a></li> <li><a href="/wiki/Output_coupler" title="Output coupler">Output coupler</a></li> <li><a href="/wiki/Q-switching" title="Q-switching">Q-switching</a></li></ul> </div></td></tr><tr><td class="navbox-abovebelow" colspan="2" style="font-weight: bold;"><div> <ul><li><img alt="" src="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/16px-Symbol_category_class.svg.png" decoding="async" width="16" height="16" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/23px-Symbol_category_class.svg.png 1.5x, //upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/31px-Symbol_category_class.svg.png 2x" data-file-width="180" data-file-height="185" /> <a href="/wiki/Category:Lasers" title="Category:Lasers">Category</a></li></ul> </div></td></tr></tbody></table></div> <div class="navbox-styles"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1129693374"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1236075235"></div><div role="navigation" class="navbox" aria-labelledby="Branches_of_chemistry" style="padding:3px"><table class="nowraplinks hlist mw-collapsible autocollapse navbox-inner" style="border-spacing:0;background:transparent;color:inherit"><tbody><tr><th scope="col" class="navbox-title" colspan="2"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1129693374"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1239400231"><div class="navbar plainlinks hlist navbar-mini"><ul><li class="nv-view"><a href="/wiki/Template:Branches_of_chemistry" title="Template:Branches of chemistry"><abbr title="View this template">v</abbr></a></li><li class="nv-talk"><a href="/wiki/Template_talk:Branches_of_chemistry" title="Template talk:Branches of chemistry"><abbr title="Discuss this template">t</abbr></a></li><li class="nv-edit"><a href="/wiki/Special:EditPage/Template:Branches_of_chemistry" title="Special:EditPage/Template:Branches of chemistry"><abbr title="Edit this template">e</abbr></a></li></ul></div><div id="Branches_of_chemistry" style="font-size:114%;margin:0 4em">Branches of <a href="/wiki/Chemistry" title="Chemistry">chemistry</a></div></th></tr><tr><td class="navbox-abovebelow" colspan="2"><div> <ul><li><a href="/wiki/Glossary_of_chemical_formulae" title="Glossary of chemical formulae">Glossary of chemical formulae</a></li> <li><a href="/wiki/List_of_biomolecules" title="List of biomolecules">List of biomolecules</a></li> <li><a href="/wiki/List_of_inorganic_compounds" title="List of inorganic compounds">List of inorganic compounds</a></li> <li><a href="/wiki/Periodic_table" title="Periodic table">Periodic table</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Analytical_chemistry" title="Analytical chemistry">Analytical</a></th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Instrumental_chemistry" title="Instrumental chemistry">Instrumental chemistry</a></li> <li><a href="/wiki/Electroanalytical_methods" title="Electroanalytical methods">Electroanalytical methods</a></li> <li><a href="/wiki/Spectroscopy" title="Spectroscopy">Spectroscopy</a> <ul><li><a href="/wiki/Infrared_spectroscopy" title="Infrared spectroscopy">IR</a></li> <li><a class="mw-selflink selflink">Raman</a></li> <li><a href="/wiki/Ultraviolet%E2%80%93visible_spectroscopy" title="Ultraviolet–visible spectroscopy">UV-Vis</a></li> <li><a href="/wiki/Nuclear_magnetic_resonance_spectroscopy" title="Nuclear magnetic resonance spectroscopy">NMR</a></li></ul></li> <li><a href="/wiki/Mass_spectrometry" title="Mass spectrometry">Mass spectrometry</a> <ul><li><a href="/wiki/Electron_ionization" title="Electron ionization">EI</a></li> <li><a href="/wiki/Inductively_coupled_plasma_mass_spectrometry" title="Inductively coupled plasma mass spectrometry">ICP</a></li> <li><a href="/wiki/Matrix-assisted_laser_desorption/ionization" title="Matrix-assisted laser desorption/ionization">MALDI</a></li></ul></li> <li><a href="/wiki/Separation_process" title="Separation process">Separation process</a></li> <li><a href="/wiki/Chromatography" title="Chromatography">Chromatography</a> <ul><li><a href="/wiki/Gas_chromatography" title="Gas chromatography">GC</a></li> <li><a href="/wiki/High-performance_liquid_chromatography" title="High-performance liquid chromatography">HPLC</a></li></ul></li> <li><a href="/wiki/Crystallography" title="Crystallography">Crystallography</a></li> <li><a href="/wiki/Characterization_(materials_science)" title="Characterization (materials science)">Characterization</a></li> <li><a href="/wiki/Titration" title="Titration">Titration</a></li> <li><a href="/wiki/Wet_chemistry" title="Wet chemistry">Wet chemistry</a></li> <li><a href="/wiki/Calorimetry" title="Calorimetry">Calorimetry</a></li> <li><a href="/wiki/Elemental_analysis" title="Elemental analysis">Elemental analysis</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Theoretical_chemistry" title="Theoretical chemistry">Theoretical</a></th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Quantum_chemistry" title="Quantum chemistry">Quantum chemistry</a></li> <li><a href="/wiki/Computational_chemistry" title="Computational chemistry">Computational chemistry</a> <ul><li><a href="/wiki/Mathematical_chemistry" title="Mathematical chemistry">Mathematical chemistry</a></li></ul></li> <li><a href="/wiki/Molecular_modelling" title="Molecular modelling">Molecular modelling</a></li> <li><a href="/wiki/Molecular_mechanics" title="Molecular mechanics">Molecular mechanics</a></li> <li><a href="/wiki/Molecular_dynamics" title="Molecular 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style="padding:0 0.25em"> <ul><li><a href="/wiki/History_of_chemistry" title="History of chemistry">History of chemistry</a></li> <li><a href="/wiki/Nobel_Prize_in_Chemistry" title="Nobel Prize in Chemistry">Nobel Prize in Chemistry</a></li> <li><a href="/wiki/Timeline_of_chemistry" title="Timeline of chemistry">Timeline of chemistry</a> <ul><li><a href="/wiki/Discovery_of_chemical_elements" title="Discovery of chemical elements">of element discoveries</a></li></ul></li> <li>"<a href="/wiki/The_central_science" title="The central science">The central science</a>"</li> <li><a href="/wiki/Chemical_reaction" title="Chemical reaction">Chemical reaction</a> <ul><li><a href="/wiki/Catalysis" title="Catalysis">Catalysis</a></li></ul></li> <li><a href="/wiki/Chemical_element" title="Chemical element">Chemical element</a></li> <li><a href="/wiki/Chemical_compound" title="Chemical compound">Chemical compound</a></li> <li><a href="/wiki/Atom" title="Atom">Atom</a></li> <li><a href="/wiki/Molecule" title="Molecule">Molecule</a></li> <li><a href="/wiki/Ion" title="Ion">Ion</a></li> <li><a href="/wiki/Chemical_substance" title="Chemical substance">Chemical substance</a></li> <li><a href="/wiki/Chemical_bond" title="Chemical bond">Chemical bond</a></li> <li><a href="/wiki/Alchemy" title="Alchemy">Alchemy</a></li> <li><a href="/wiki/Quantum_mechanics" title="Quantum mechanics">Quantum mechanics</a></li></ul> </div></td></tr><tr><td class="navbox-abovebelow" colspan="2"><div> <ul><li><img alt="" src="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/16px-Symbol_category_class.svg.png" decoding="async" width="16" height="16" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/23px-Symbol_category_class.svg.png 1.5x, //upload.wikimedia.org/wikipedia/en/thumb/9/96/Symbol_category_class.svg/31px-Symbol_category_class.svg.png 2x" data-file-width="180" data-file-height="185" /> <a 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