Mass cytometry/CyTOF Antibodies

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><meta name="applicable-device" content="pc,mobile" /> <meta name="description" content="Limit spectral overlap and detect 40 cellular targets from an extensive selection of CyTOF ready and cited antibodies." /> <meta name="keywords" content="Mass cytometry, CyTOF Antibodies" /> <link rel="canonical" href="https://www.bio-techne.com/applications/mass-cytometry" /> <meta property="og:site_name" content="Bio-Techne" /> <meta property="og:type" content="website" /> <meta property="og:url" content="https://www.bio-techne.com/applications/mass-cytometry" /> <meta property="og:image" content="https://resources.bio-techne.com/bio-techne-assets/images/brands/bio-techne-og.png" /> <meta name="product-category" content="Antibodies" /> <meta name="brand" content="Novus Biologicals" /> <meta name="revision-date" content="1731341242" /> <meta name="published-date" content="1695130178" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <script type="application/ld+json">{ "@context": "https://schema.org", "@graph": [ { "@type": "WebPage", "@id": "https://www.bio-techne.com/applications/mass-cytometry", "breadcrumb": { "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bio-techne.com/" }, { "@type": "ListItem", "position": 2, "name": "Applications", "item": "https://www.bio-techne.com/applications" } ] } } ] }</script> <meta http-equiv="x-dns-prefetch-control" content="on" /> <meta http-equiv="x-ua-compatible" content="ie=edge" /> <link rel="alternate" hreflang="en" href="https://www.bio-techne.com/applications/mass-cytometry" /> <link rel="alternate" hreflang="zh-hans" href="https://www.bio-techne.com/cn/applications/mass-cytometry" /> <link rel="icon" href="/themes/custom/bio_techne_global/favicon.ico" type="image/vnd.microsoft.icon" /> <link rel="preconnect" href="https://resources.bio-techne.com" /> <link rel="preconnect" href="https://resources.tocris.com" /> <link rel="preconnect" href="https://i.icomoon.io" /> <link rel="preconnect" href="https://cdn.icomoon.io" /> <link rel="dns-prefetch" href="https://connect.facebook.net" /> <link rel="dns-prefetch" href="https://resources.bio-techne.com" /> <link rel="dns-prefetch" href="https://snap.licdn.com" /> <link rel="dns-prefetch" href="https://www.google-analytics.com" /> <link rel="dns-prefetch" href="https://munchkin.marketo.net" /> <link rel="dns-prefetch" href="https://resources.tocris.com" /> <link rel="dns-prefetch" href="https://i.icomoon.io" /> <link rel="dns-prefetch" href="https://cdn.icomoon.io" /> <title>Mass cytometry/CyTOF Antibodies</title> <link rel="stylesheet" media="all" href="/sites/default/files/css/css_656_2vqy1Km2MYNBaMfnvrWzlHUsrQzYL6gRawV6hgo.css?delta=0&language=en&theme=bio_techne_global&include=eJxNzmFyhDAIBeALJeZIDImsUjFkIWnH21fXtttfb_hmeGDkTavzJ0HHLAQPlk6W7M_jy4Mf3mlPGZ1CZoVOZa0Ei2hGSXdE74dwXcLHc5AdMBi8ca1n30-GoroxQdXODy7YWWu6Lf63mEXLFhoaLoZt9TTbaCjTW6ZR28jCvtIcBA8dHfJgmc9r_UuLCjiVqyw003mUfs5oZU13QMbrnX0nKwTPwWVTu5Z_Lb5ter3zDT02d0M" /> <link rel="stylesheet" media="all" href="/sites/default/files/css/css_d9gn2GRzRb_AMxXfgjVqY5zLMXvuwkXc1qKvoeAAIxE.css?delta=1&language=en&theme=bio_techne_global&include=eJxNzmFyhDAIBeALJeZIDImsUjFkIWnH21fXtttfb_hmeGDkTavzJ0HHLAQPlk6W7M_jy4Mf3mlPGZ1CZoVOZa0Ei2hGSXdE74dwXcLHc5AdMBi8ca1n30-GoroxQdXODy7YWWu6Lf63mEXLFhoaLoZt9TTbaCjTW6ZR28jCvtIcBA8dHfJgmc9r_UuLCjiVqyw003mUfs5oZU13QMbrnX0nKwTPwWVTu5Z_Lb5ter3zDT02d0M" /> <script type="application/json" data-drupal-selector="drupal-settings-json">{"path":{"baseUrl":"\/","pathPrefix":"","currentPath":"node\/10071","currentPathIsAdmin":false,"isFront":false,"currentLanguage":"en"},"pluralDelimiter":"\u0003","suppressDeprecationErrors":true,"gtm":{"tagId":null,"settings":{"data_layer":"dataLayer","include_environment":false},"tagIds":["GTM-N2MWQDR"]},"gtag":{"tagId":"","consentMode":false,"otherIds":[],"events":[],"additionalConfigInfo":[]},"ajaxPageState":{"libraries":"eJxtku2a4yAIhW9I6yX5oKEJWyMuYmcyV7-kmaazu_3jx8shOSCJOCrmpWKcCycoITFrV4EW81Rd-i9-bL7rVqjObwSUeWV-l1rga_OFYXoTW3Ei8F5wZUV_pwl5V32LCt0xwoxVwwn9Dv0D2jEJyOZSYV4FIS-x0SeWMIFCgQ3Ft9EXFJc0Yl2gZpwimtMVJWNI6p_Un9R3UvwwJx7v9pPuTvmpOMyfgfh7UL6xTCgvzYtdzF6-mZpvhLGy0pUyKHENB_M_mT_UZwUh4QJ3YukOC3SlHBvoEkdHiZAzD_MYvg_WSB1SfbP-WHfqzc3Mc8GoMIfZln_vF_gFn3_D1VFVlPpwA4W-nlbt-7L5jgWzHi59K5Bx4WJVOnPLQ2MatF-DfnDmEruJLd01EJhtvJYeJhkNyuVFLqO2kQrZS02uCU8jqyWC5CUcW0wgTrA3rn2fCYVkfq9UzGh4cf_grm9dcQ0JOrpHm6heWdajjh34H-A50n8AW9w07Q","theme":"bio_techne_global","theme_token":null},"ajaxTrustedUrl":[],"dataLayer":{"defaultLang":"en","languages":{"en":{"id":"en","name":"English","direction":"ltr","weight":0},"zh-hans":{"id":"zh-hans","name":"Chinese, Simplified","direction":"ltr","weight":1},"ja":{"id":"ja","name":"Japanese","direction":"ltr","weight":2}}},"internationalization":{"origin":{"id":"sg","label":"SG","country_name":"Singapore","alternate_spellings":"SG","country_enabled":true,"country_encased":false,"commerce_enabled":false,"credit_cards_enabled":true,"region":"North America - ROW","currency":"USD","localities":"","phone_pattern":"","country_shipping":"0","shipping_delay":3,"country_contact_message":"","product_block_message":"","default_language_setting":"none","default_contact_email":""},"languagePrefix":"\/"},"bloomreach_pixel":{"br_info":{"acct_id":"6368","domain_key":"biotechne"},"page_info":{"ptype":"content"},"order_info":{"order_id":""},"user_info":{"uid":0},"searchbar":{"enabled":"1","form":".product-search-form","input":"#edit-keywords"},"atc":{"enabled":"1","form":".add-to-cart-block"}},"user":{"uid":0,"permissionsHash":"085655eba91c5b5dd7808236555504ceb824f9fddd71e3c0265b4003df711142"}}</script> <script src="/sites/default/files/js/js_VXwrJf_GoEAY9I4SWeDh30VrU0-JgQhDzf_eoQcyzA4.js?scope=header&delta=0&language=en&theme=bio_techne_global&include=eJxtkW2SwyAIhi8k65EcVKpujWSQZJuefm3TZLo7_QU8MLx8-MJKITdytazkMFFT6w8IDwhPOFwvKJuJqFhxI7GeMq6FpRuq2LUEN6Nmt3QShyHw0rTblwNCukiDeTQbrdrVDBH3kk6VPVbrmbWr4OxCbB_yu4Gu22iQTGJOlZxiskmnvyGm__EXfuPNeHXUMrZA0VHgaSIJZL3CQeGk0IvST4kEtI79uznLz4p9oJHgayHXWMulBNTCze4M3hn4ymHsXZknIQzZzeVG1Z4HhXnpmeTD5hPFgjBuOI3HwDqGYjMLxyWo64QSst2N8yimNCVpT02s5X4MNL4gG3SqFHSfBeaKgTLX-FG14n2DyhjN86WlXVimvdsDwBs4nvIL0JnnFQ"></script> <script src="/modules/contrib/google_tag/js/gtm.js?snbwxk"></script> <script src="/modules/contrib/google_tag/js/gtag.js?snbwxk"></script> <script src="/sites/default/files/js/js_noO6VL3JUON9fTBkbqcAjEkLsfpTqx94dWIfXpNxuMg.js?scope=header&delta=3&language=en&theme=bio_techne_global&include=eJxtkW2SwyAIhi8k65EcVKpujWSQZJuefm3TZLo7_QU8MLx8-MJKITdytazkMFFT6w8IDwhPOFwvKJuJqFhxI7GeMq6FpRuq2LUEN6Nmt3QShyHw0rTblwNCukiDeTQbrdrVDBH3kk6VPVbrmbWr4OxCbB_yu4Gu22iQTGJOlZxiskmnvyGm__EXfuPNeHXUMrZA0VHgaSIJZL3CQeGk0IvST4kEtI79uznLz4p9oJHgayHXWMulBNTCze4M3hn4ymHsXZknIQzZzeVG1Z4HhXnpmeTD5hPFgjBuOI3HwDqGYjMLxyWo64QSst2N8yimNCVpT02s5X4MNL4gG3SqFHSfBeaKgTLX-FG14n2DyhjN86WlXVimvdsDwBs4nvIL0JnnFQ"></script> </head> <body class="node-10071 node-type-page"> <a href="#main-content" class="visually-hidden focusable"> Skip to main content </a> <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-N2MWQDR" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript> <div class="dialog-off-canvas-main-canvas" data-off-canvas-main-canvas> <div id="page-wrapper"> <div id="page"> <header id="header" class="header" role="banner" aria-label="Site header"> <div class="section layout-container clearfix"> <section id="site-header__top"> <div class="container-fluid container-wide"> <div class="row m___navigation-utility-top"> <div class="col-xl-2 col-lg-12 text-xl-left text-lg-center"> <div id="site-header__logo" class="valign d-none d-lg-block mb-md-3"> <div> <div id="block-sitebranding" class="site-branding system_branding_block"> <a href="/" title="Home" rel="home" class="site-branding__logo"> <img width="160" height="28" src="/themes/custom/bio_techne_global/logo.svg" alt="Home" /> </a> </div> </div> </div> </div> <div class="col d-none d-xl-block text-lg-left text-md-center"> <div class="utility-bar-tagline rotating-text position-absolute"> <p class="ml-xl-4 bt-px_groteskbold text-white mb-0"> Global Developer, Manufacturer, and Supplier of High-Quality<br/> Reagents, Analytical Instruments, and Precision Diagnostics. </p> </div> <div class="utility-bar-brands rotating-text position-absolute ml-xl-4 text-white mb-0"> <div class="row"> <div class="col-2"> <div class="bt-px_groteskbold valign utility-bar-includes">INCLUDES</div> </div> <div class="col-lg-10 col-md-auto bt-inter"> <span class="mr-3">R&D Systems<sup>TM</sup></span> <span class="mr-3">Novus Biologicals<sup>TM</sup></span> <span class="mr-3">Tocris Bioscience<sup>TM</sup></span><br/> <span class="mr-3">ProteinSimple<sup>TM</sup></span> <span class="mr-3">ACD<sup>TM</sup></span> <span class="mr-3">ExosomeDx<sup>TM</sup></span> <span class="mr-3">Asuragen<sup style="font-size:11px;">®</sup></span> <span>Lunaphore<sup>TM</sup></span> </div> </div> </div> </div> <div class="col-xl-auto col-lg-12"> <div class="row"> <div class="col text-xl-left text-center"> </div> </div> <div class="row"> <div class="col text-xl-left text-center"> <nav id="site-header-top__left m___navigation-language"> <div> <div id="block-i18ncountryselect" class="i18n_country_select"> <div id="country-select-block-placeholder"> <div id="country-select-block-spinner" class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </div> </div> </div> </nav> </div> </div> </div> </div> </div> </section> <div id="header-bg" class="bg-bt-secondary"> <section id="site-header__middle"> <div class="container-fluid container-wide"> <div class="row search-row"> <div id="hamburger-col" class="col-3 col-sm-3 col-md-2 d-block d-lg-none"> <button class="navbar-toggler collapsed" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation"> <span class="icon-bar top-bar"></span> <span class="icon-bar middle-bar"></span> <span class="icon-bar bottom-bar"></span> </button> </div> <div class="col-6 col-sm-6 col-md-8 d-block d-lg-none text-center"> <div id="site-header__logo" class="vertical-align mb-3"> <div> <div id="block-sitebranding" class="site-branding system_branding_block"> <a href="/" title="Home" rel="home" class="site-branding__logo"> <img width="160" height="28" src="/themes/custom/bio_techne_global/logo.svg" alt="Home" /> </a> </div> </div> </div> </div> <div id="tools-col" class="col-3 col-md-12 col-lg-auto order-sm-1 order-md-12 order-lg-12"> <div id="site-header__tools" class="vertical-align"> <div class="row"> <div class="col-md-12"> <div class="d-flex justify-content-end justify-content-md-center justify-content-lg-end"> <div class="text-right order-3 cart-not-available user_cart_information_block" id="block-biotechnecartinformation"> <span ></span> </div> <div class="mr-3 d-none d-md-block order-2 block-quickorderblock quick_order_block" id="block-quickorderblock"> </div> <div class="mr-3 order-1 user_account_information_block" id="block-biotechneuserinformation"> <a class="btn btn-primary" rel="nofollow" href="/account/login" role="button"> <span class="icon icon-bt-icon-user"></span> Sign In / Register </a> </div> </div> </div> </div> </div> </div> <div class="col order-sm-12 order-md-1 order-lg-1"> <div id="site-header__search">  <section role="search" class="vertical-align"> <div> <div class="block-product-search-form-block product_search_form_block" id="block-product-search-form-block"> <div class="row"> <div class="col-sm-12 search-bar mb-md-4"> <form class="product-search-form" action="/search" method="get" id="product-search-form" accept-charset="UTF-8" data-date="2024-11-23 12:11:22"> <div class="container-inline input-group" id="edit-container"> <div class="js-form-type-select"> <select class="dropdown-select text-dark-gray js-search-section-dropdown mr-2 pl-4 pr-5" id="edit-search-type" name="search_type" aria-label="Select search type"> <option value="products">Products</option> <option value="resources">Resources</option> </select> </div> <input placeholder="Search keyword, molecule name, target, catalog number, or product type..." autocomplete="off" class="form-control js-search-bar form-autocomplete form-text required ui-autocomplete-input" title="Search keyword, molecule name, target, catalog number, or product type..." data-autocomplete-path="/ajax/product-search/auto-suggest" type="text" id="product-search-keywords" name="keywords" value="" size="60" maxlength="256" required="required" aria-required="true" data-once="autocomplete" spellcheck="false"> <div class="form-actions" id="edit-actions"> <div class="input-group-append"> <input class="form-submit" type="submit" value=""> </div> </div> </div> </form> </div> </div> </div> </div> </section> </div> </div> </div> </div> </section> <section id="site-header__bottom"> <div class="container-fluid container-wide"> <div class="row"> <div class="col-md-12">  <nav id="main-nav"> <div> <div id="block-bio-techne-global-mainmenublock" class="main_menu_block"> <nav role="navigation" aria-labelledby="block-main-menu" id="block-main-menu" class="contextual-region navbar navbar-expand-md navbar-dark"> <div class="collapse navbar-collapse" id="navbarSupportedContent" data-date="2024-11-22 05:11:30"> <ul class="navbar-nav ml-auto"> <li class="nav-item dropdown d-md-none"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-quick-order" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Quick Order <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-quick-order" aria-labelledby="navbarDropdown-quick-order"> <div class="row"> <a href="/commerce/quickorder" class="dropdown-item text-wrap" title="Quick order form" data-drupal-link-system-path="commerce/quickorder">Quick Order Form</a> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-research-products" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Research Products <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-research-products" aria-labelledby="navbarDropdown-research-products"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Reagents & Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/reagents/antibodies" class="dropdown-item text-wrap" data-drupal-link-system-path="node/276">Antibodies</a> <a href="/reagents/cell-culture-reagents" class="dropdown-item text-wrap" data-drupal-link-system-path="node/156">Cell Culture Reagents</a> <a href="/reagents/compound-libraries" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1096">Compound Libraries</a> <a href="/reagents/elisa-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/176">ELISA Kits</a> <a href="/reagents/elispot-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/141">ELISpot Kits</a> <a href="/reagents/enzymes" class="dropdown-item text-wrap" data-drupal-link-system-path="node/246">Enzymes</a> <a href="/reagents/fluorescent-dyes-probes" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6191">Fluorescent Dyes and Probes</a> <a href="/reagents/luminex-assays" class="dropdown-item text-wrap" data-drupal-link-system-path="node/181">Luminex Assays</a> <a href="/reagents/proteins" class="dropdown-item text-wrap" data-drupal-link-system-path="node/236">Proteins</a> <a href="/reagents/proteome-profiler-antibody-arrays" class="dropdown-item text-wrap" data-drupal-link-system-path="node/206">Proteome Profiler Antibody Arrays</a> <a href="/reagents/rnascope-ish-technology" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1626">RNAscope ISH Probes & Assays</a> <a href="/reagents/simple-plex-immunoassays" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1531">Simple Plex Immunoassays</a> <a href="/reagents/small-molecules-and-peptides" class="dropdown-item text-wrap" data-drupal-link-system-path="node/371">Small Molecules & Inhibitors</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> GMP Products<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/gmp-products/gmp-proteins" class="dropdown-item text-wrap" data-drupal-link-system-path="node/511">GMP Proteins</a> <a href="/gmp-products/gmp-small-molecules" class="dropdown-item text-wrap" data-drupal-link-system-path="node/366">GMP Small Molecules</a> <a href="/gmp-products/gmp-antibodies" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13266">GMP Antibodies</a> <a href="/gmp-products/gmp-capabilities" class="dropdown-item text-wrap" title="GMP Capabilities" data-drupal-link-system-path="node/1071">GMP Capabilities</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" title="Our capillary electrophoresis instruments (icIEF and CE-SDS) provide reproducible quantitative analysis for thorough therapeutic protein characterization." class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single-Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> <a href="/applications/crispr" class="dropdown-item text-wrap" data-drupal-link-system-path="node/9356">CRISPR</a> <a href="/applications/electrophysiology" class="dropdown-item text-wrap" data-drupal-link-system-path="node/426">Electrophysiology</a> <a href="/applications/flow-cytometry" class="dropdown-item text-wrap" data-drupal-link-system-path="node/321">Flow Cytometry</a> <a href="/applications/imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10181">Imaging </a> <a href="/applications/immunoassays" class="dropdown-item text-wrap" data-drupal-link-system-path="node/496">Immunoassays</a> <a href="/resources/instrument-applications" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3341">Instrument Applications</a> <a href="/applications/mass-cytometry" class="dropdown-item text-wrap is-active" data-drupal-link-system-path="node/10071" aria-current="page">Mass Cytometry (CyTOF)</a> <a href="/applications/spatial-biology" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13416">Spatial Biology</a> <a href="/applications/vaccine-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/566">Vaccine Development</a> <a href="/applications/western-blotting" class="dropdown-item text-wrap" data-drupal-link-system-path="node/281">Western Blotting</a> <a href="/applications" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6736">View All Applications</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cancer" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8791">Cancer</a> <a href="/research-areas/cell-biology" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10661">Cell Biology</a> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/research-areas/coronavirus" class="dropdown-item text-wrap" data-drupal-link-system-path="node/576">COVID-19</a> <a href="/research-areas/epigenetics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/9511">Epigenetics</a> <a href="/research-areas/hypoxia" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8846">Hypoxia</a> <a href="/research-areas/immunology" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10601">Immunology</a> <a href="/research-areas/immuno-oncology" class="dropdown-item text-wrap" data-drupal-link-system-path="node/381">Immuno-Oncology</a> <a href="/research-areas/metabolism" class="dropdown-item text-wrap" data-drupal-link-system-path="node/406">Metabolism</a> <a href="/research-areas/neuroscience" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10591">Neuroscience</a> <a href="/research-areas/organoids-3d-culture" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1452">Organoid and 3-D Culture</a> <a href="/research-areas/stem-cells" class="dropdown-item text-wrap" data-drupal-link-system-path="node/161">Stem Cells</a> <a href="/research-areas/targeted-protein-degradation" class="dropdown-item text-wrap" data-drupal-link-system-path="node/376">Targeted Protein Degradation</a> <a href="/research-areas" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6741">View All Research Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Reagents & Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3576">Animal-Free Cell Culture</a> <a href="/reagents/elisa-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/176">ELISA Kits</a> <a href="/therapeutic-manufacturing/simple-plex-assays" class="dropdown-item text-wrap" title="Bioprocess impurities and immunotitration" data-drupal-link-system-path="node/1311">Simple Plex Immunoassays</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> GMP Products<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/gmp-products/gmp-proteins" class="dropdown-item text-wrap" data-drupal-link-system-path="node/511">GMP Proteins</a> <a href="/gmp-products/gmp-small-molecules" class="dropdown-item text-wrap" data-drupal-link-system-path="node/366">GMP Small Molecules</a> <a href="/gmp-products/gmp-antibodies" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13266">GMP Antibodies</a> <a href="/gmp-products/gmp-capabilities" class="dropdown-item text-wrap" title="GMP Capabilities" data-drupal-link-system-path="node/1071">GMP Capabilities</a> <a href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2066">Immuno-Oncology</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-diagnostics" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Diagnostics <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-diagnostics" aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1281">Custom Cell & Gene Therapy Services</a> <a href="/services/custom-compound-library-service" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2126">Custom Compound Library Services</a> <a href="/services/custom-elisa-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/261">Custom ELISA Services</a> <a href="/services/gene-engineering-services-tcbuster" class="dropdown-item text-wrap" title="Gene Engineering Services: Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup Tool</a> <a href="/resources/rnascope-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/rnascope-citations">RNAscope Citations</a> <a href="/resources/simple-plex-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-plex-panel-builder">Simple Plex Panel Builder</a> <a href="/resources/simple-western-kit-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-kit-builder">Simple Western Kit Builder</a> <a href="/resources/simple-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-antibody-database">Simple Western Antibody Database</a> <a href="/resources/single-cell-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/single-cell-western-antibody-database">Single-Cell Western Antibody Database</a> <a href="/resources/spectra-viewer" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6176">Spectra Viewer</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Scientific Resources<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/blogs" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/blogs">Blogs</a> <a href="/resources/literature" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/literature">Literature</a> <a href="/resources/podcasts/biotech-podcast-back-of-the-napkin" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8601">Podcasts</a> <a href="/resources/protocols-troubleshooting" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/protocols-troubleshooting">Protocols & Troubleshooting</a> <a href="/resources/scientific-articles" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/scientific-articles">Scientific Articles</a> <a href="/resources/signal-transduction-pathways" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/signal-transduction-pathways">Signal Transduction Pathways</a> <a href="/resources/testimonials" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/testimonials">Testimonials & Interviews</a> <a href="/resources/webinars" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1596">Webinars</a> <a href="/resources/videos" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/videos">Videos</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Resources<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/instrument-applications" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3341">Instrument Applications</a> <a href="/resources/instrument-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/instrument-citations">Instrument Citations</a> <a href="/resources/protocols" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/protocols">Instrument Protocols</a> <a href="/resources/instrument-software-download-center" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/instrument-software-download-center">Instrument Software</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-about" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> About <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-about" aria-labelledby="navbarDropdown-about"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> About Us<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/welcome-to-bio-techne" class="dropdown-item text-wrap" data-drupal-link-system-path="node/876">Welcome to Bio-Techne.com!</a> <a href="/about/about-bio-techne" class="dropdown-item text-wrap" data-drupal-link-system-path="node/16">About Bio-Techne</a> <a href="/brands" class="dropdown-item text-wrap" data-drupal-link-system-path="node/46">Bio-Techne Brands</a> <a href="/about/corporate-and-social-responsibility" class="dropdown-item text-wrap" data-drupal-link-system-path="node/61">Corporate & Social Responsibility</a> <a href="https://investors.bio-techne.com/" target="_blank" class="dropdown-item text-wrap">Investor Relations</a> <a href="/about/quality" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6056">Quality</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Latest at Bio-Techne<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/about/careers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/21">Careers</a> <a href="/about/events" class="dropdown-item text-wrap" data-drupal-link-system-path="node/786">Events</a> <a href="/programs" class="dropdown-item text-wrap" data-drupal-link-system-path="node/4621">Programs & Promotions</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Support<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/support/contact-us" class="dropdown-item text-wrap" data-drupal-link-system-path="node/96">Contact & Support</a> <a href="/distributors" class="dropdown-item text-wrap" data-drupal-link-system-path="node/9936">Distributors</a> </div> </div> </div> </div> </div> </li> </ul> </div> </nav> </div> </div> </nav> </div> </div> </div> </section> </div> </div> </header> </div> <div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/applications">Applications</a> </li> <li> Mass Cytometry (CyTOF) </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h1>Mass Cytometry (CyTOF)</h1> <p><a href="#Mass Cytometry Basics">Mass Cytometry Basics</a> | <a href="#Advantages">Advantages </a>| <a href="#CyTOF Antibodies">CyTOF Antibodies</a> | <a href="#CyTOF Webinar">CyTOF Webinar</a> | <a href="#Citations">Citations</a></p> <p>Mass cytometry, also known as cytometry by time-of-flight (CyTOF), fuses the experimental platforms of <a href="/applications/imaging/flow-cytometry">flow cytometry</a> and elemental mass spectrometry. Mass cytometers require antibodies conjugated to rare earth metal isotopes of defined atomic mass instead of fluorochromes. Although throughput and sensitivity is less than conventional flow cytometry (10x less), the unique mass signatures of these stable metal isotopes by time-of-flight mass spectrometry (TOFMS) improves specificity by accurately discriminating metal isotopes of different atomic masses without the need for isotype controls. This unique signature of metals provides simultaneous identification of over 40 targets per cell, providing greater capabilities of phenotyping cells than flow cytometry (with just over 20 parameters using multi-color panels).</p></div> </div> </div> <div> <div class="paragraph paragraph--type--individual-button mb-4 paragraph--view-mode--default w-100"> <div class="row"> <div class="col"> <a href="/search?search_type=products&keywords=cytof" class="btn btn-primary" type="link" > View our CyTOF-ready Antibodies </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="Mass Cytometry Basics"></a>Mass Cytometry Basics</h2> <p>To quantify the expression level of proteins on individual cells, metal isotopes can be <a href="/reagents/antibodies/conjugated-primary-antibodies">conjugated to primary antibodies</a> for mass cytometry analysis. Fixed, stained cells are passed in a single-cell suspension into a nebulizer as droplets into the mass cytometer. Cells are then injected into an argon flow chamber and exposed to a plasma torch, vaporizing cells into an ionized cloud. The ion clouds are passed through a quadrupole to enrich the heavy-metal reporter ion while removing impurities. Ions are separated by their mass-to-charge ratio in a time-of-flight mass spectrometer. Cell particles come out according to the conjugated atomic mass, where heavier mass-to-charge metal tags have a longer time-of-flight. Every column represents a distinct isotope measured and each row represents a single mass scan of the detector.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/mass-cytometry-cytof-banner1.jpg" alt="Mass cytometry uses metal-conjugated antibodies to provide simultaneous identification of over 40 targets per cell" loading="lazy" > </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><strong>Figure 1: Mass Cytometry Basics.  A</strong>. Antibodies conjugated to metal isotopes; <strong>B</strong>. Single-cell suspension carried into nebulizer of the mass cytometer: <strong>C</strong>. Cells injected into an argon flow chamber are exposed to a plasma torch; <strong>D</strong>. Metal tagged vaporized cells form ion clouds sent through Quadrupole for purification and concentration; <strong>E</strong>. Times of flight (TOFs) of the ions from the plasma are measured by a mass spectrometer detector; <strong>F</strong>. Individualized cells are profiled by atomic mass (i.e. lighter metals arrive first)</p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="Advantages"></a>Advantages Over Fluorescence Flow Cytometry</h2> <ul> <li><strong>Reduce Spectral Overlap</strong>: TOFMS accurately discriminates metal isotopes of different atomic masses without channel overlap.</li> <li><strong>Minimal compensation</strong>: No need for fluorescence compensation and minimal background observed from the instrument if cleaned properly.</li> <li><strong>Eliminate Autofluorescence Background</strong>: Absence of interference from cellular autofluorescence as cells do not contain metals intrinsically.</li> <li><strong>Panel design</strong>: 40+ channels available in CyTOF, increasing flexibility and ease in panel design.</li> <li><strong>Improve Molecular Profiling</strong>: Simultaneous detection and resolution of more targets than is feasible with conventional flow cytometry, making TOFMS ideal for testing rare or precious samples.</li> </ul></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="CyTOF Antibodies"></a>Bio-Techne CyTOF Antibodies</h2> <p>We have a wide range of antibodies that are bottled <a href="/search?search_type=products&keywords=Primary+Antibodies+Azide+and+BSA+Free">BSA and azide-free</a> in a CyTOF-ready formulation for metal conjugation. Additional benefits for established conjugation methods include:</p> <ul> <li>Liquid formulation or lyophilized for reconstitution at high concentration and improved conjugation efficiency.</li> <li>No need for a buffer exchange step prior to conjugation.</li> <li>Over 2800 CyTOF-ready antibodies available.</li> <li>Also available conjugated to fluorochromes for fluorescence flow cytometry.</li> </ul> <p> </p> <p><strong>Choose from</strong>:</p> <ul> <li><a href="/search?search_type=products&keywords=antibodies&applications=CyTOF-ready">CyTOF-ready Antibodies</a></li> <li><a href="/search?keywords=Flow%20Cytometry">Flow Cytometry Antibodies</a></li> </ul></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Other Considerations of Mass Cytometry</h2> <p><strong>Antibody conjugation: </strong>Metal conjugation antibody kits can be laborious and require meticulous attention to detail.</p> <p><strong>Consumables cost:</strong> The use of rare earth metals in metal labeling antibody kits and required use of inert gases for all experiments make up a significant consumables cost. </p> <p><strong>Slower acquisition: </strong>Compared to fluorescence flow cytometry, CyTOF experiments run at a slower rate (1,000 cells/sec vs 10,000 cells/sec). This leads to increased costs for instrument use and therefore can become cost prohibitive. However, the costs are offset by the capability of CyTOF to do extensive molecular profiling, which produces more data from each cell.</p> <p><strong>No downstream analysis: </strong>Due to the nature of the application, cells are vaporized into an ionized cloud, losing the capabilities to sort cells for further analysis or downstream applications.</p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Imaging Mass Cytometry</h2> <p>Further advanced technology known as imaging mass cytometry fuses immunohistochemistry and immunocytochemistry imaging techniques with mass cytometry. In imaging mass cytometry, metal conjugated antibodies are used on paraffin-embedded tissue sections. Laser ablation separates cells from the fixed tissue slide (fresh or frozen) and the volatized cells are carried by inert gas into the mass cytometer for analysis described above.</p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2><a name="CyTOF Webinar"></a>Mass Cytometry Webinar</h2> <p>In the Bio-Techne webinar, “Single-Cell Profiling with Mass Cytometry: An Overview of Technology and Current Research Applications On-Demand”, learn more about CyTOF from a leading expert.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="QormJUuC_-0" params="rel=0" style="background-image: url('https://img.youtube.com/vi/QormJUuC_-0/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/QormJUuC_-0?autoplay=1" class="lty-playbtn" title="Single-cell Profiling with Mass Cytometry, a Variation of Flow Cytometry"> <span class="lyt-visually-hidden">Single-cell Profiling with Mass Cytometry, a Variation of Flow Cytometry</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><strong><a name="Citations"></a>Selected Citations of CyTOF-Reported Antibodies</strong></h2> <p>Application of mass cytometry (CyTOF) for functional and phenotypic analysis of natural killer cells. Kay, A. et al. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304457/" target="_blank">Methods Mol. Biol. (2016). 1441: 13–26.</a> (Catalog # <a href="/p/antibodies/human-kir2dl1-kir2ds5-antibody-143211_mab1844">MAB1844</a> , <a href="/p/antibodies/human-cxcr6-antibody-56811_mab699">MAB699</a>, <a href="/p/antibodies/human-kir2dl3-cd158b2-antibody-180701_mab2014">MAB2014</a>, <a href="/p/antibodies/human-kir2dl4-cd158d-antibody-181703_mab2238">MAB2238</a>, <a href="/p/antibodies/human-nkg2c-cd159c-antibody-134522_mab1381">MAB1381</a>)</p> <p>High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry. Nair, N. et al. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618273/" target="_blank">Mucosal Immunol. (2016) 9: 68.</a> (Catalog # <a href="/p/antibodies/human-ccr7-antibody-150503_mab197">MAB197</a> , <a href="/p/antibodies/human-cxcr4-antibody-12g5_mab170">MAB170</a> )</p> <p>Immune profiles to predict response to desensitization therapy in highly HLA-sensitized kidney transplant candidates. Yabu, J.M. et al. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831845/" target="_blank">PLoS ONE (2016) 11: e0153355.</a> (Catalog # <a href="/p/antibodies/human-ccr7-antibody-150503_mab197">MAB197</a>, <a href="/p/antibodies/human-lilrb1-cd85j-ilt2-antibody-292319_mab20172">MAB20172</a>)</p> <p>Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. Mei, H. et al. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323739/" target="_blank">J. Immunol. (2015) 194:2022.</a> (Catalog # <a href="/p/antibodies/human-ccr7-antibody-150503_mab197">MAB197</a>, <a href="/p/antibodies/human-lilrb1-cd85j-ilt2-antibody-292319_mab20172">MAB20172</a>)</p> <p>Regulation of adaptive NK cells and CD8 T cells by HLA-C correlates with allogeneic hematopoietic cell transplantation and with cytomegalovirus reactivation. Horowitz, A. et al. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610859/" target="_blank">J. Immunol. (2015) 195:4524.</a> (Catalog # <a href="/p/antibodies/human-nkg2c-cd159c-antibody-134591_mab138">MAB138</a>, <a href="/p/antibodies/human-kir2dl1-kir2ds5-antibody-143211_mab1844">MAB1844</a>, <a href="/p/antibodies/human-kir2dl3-cd158b2-antibody-180701_mab2014">MAB2014</a>)</p></div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> </div> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div class="col-xl-9 col-lg-auto"> <p class="ml-xl-4 bt-px_groteskbold footer-tagline">Global Developer, Manufacturer, and Supplier of High-Quality Reagents, Analytical Instruments, and Precision Diagnostics.</p> </div> <div class="included-brands col"> <span class="font-weight-bold mr-3 bt-inter_regular">INCLUDES</span> <span class="mr-3 bt-inter_regular">R&D Systems™</span> <span class="mr-3 bt-inter_regular">Novus Biologicals™</span> <span class="mr-3 bt-inter_regular">Tocris Bioscience™</span> <span class="mr-3 bt-inter_regular">ProteinSimple™</span> <span class="mr-3 bt-inter_regular">ACD™</span> <span class="mr-3 bt-inter_regular">ExosomeDx™</span> <span class="mr-3 bt-inter_regular">Asuragen™</span> <span class="mr-3 bt-inter_regular">Lunaphore™</span> </div> </div> </div> </div> <div id="site-footer__top" class=" pt-4"> <div class="container-wide container-fluid pt-3"> <div> <div class="col-md-12"> <footer> <div> <div class="row"> <div class="col-xl-3 col-md-6 col-xs-12 p-0 text-white bt-inter_regular"> <div> <div id="block-footercolumn1" class="block_content:8da4adc8-094f-4085-8706-613703de4da6"> <div><h4>CONTACT INFORMATION</h4> <p>614 McKinley Place NE<br /> Minneapolis, MN 55413<br /> USA</p> <p>TEL <a href="tel:612 379 2956">612 379 2956</a><br /> Toll-free <a href="tel:800 343 7475">800 343 7475</a><br /> FAX 612 656 4400</p> </div> </div> </div> </div> <div class="col-xl-3 col-md-6 col-xs-12 p-0 bt-inter_regular"> <div> <nav role="navigation" aria-labelledby="block-footercolumn2-menu" id="block-footercolumn2"> <h4 id="block-footercolumn2-menu">Who We Are</h4> <ul> <li> <a href="/about/about-bio-techne" title="About Bio-Techne" data-drupal-link-system-path="node/16">About Bio-Techne</a> </li> <li> <a href="/brands" title="Brands" data-drupal-link-system-path="node/46">Bio-Techne Brands</a> </li> <li> <a href="/about/careers" title="History" data-drupal-link-system-path="node/21">Careers </a> </li> <li> <a href="/about/corporate-and-social-responsibility" title="Corporate Responsibility" data-drupal-link-system-path="node/61">Corporate & Social Responsibility </a> </li> <li> <a href="/terms-and-conditions" data-drupal-link-system-path="node/686">Terms and Conditions</a> </li> </ul> </nav> </div> </div> <div class="col-xl-3 col-md-6 col-xs-12 p-0 bt-inter_regular"> <div> <nav role="navigation" aria-labelledby="block-footercolumn3-menu" id="block-footercolumn3"> <h4 id="block-footercolumn3-menu">Investor Relations</h4> <ul> <li> <a href="https://investors.bio-techne.com/">Overview</a> </li> <li> <a href="https://investors.bio-techne.com/news-events">News / Events</a> </li> <li> <a href="https://investors.bio-techne.com/company-information">Company Information</a> </li> <li> <a href="https://investors.bio-techne.com/financial-information">Financial Information</a> </li> <li> <a href="https://investors.bio-techne.com/stock-data">Stock Data</a> </li> <li> <a href="https://investors.bio-techne.com/sec-filings">SEC Filings</a> </li> <li> <a href="https://investors.bio-techne.com/corporate-governance">Corporate Governance</a> </li> </ul> </nav> </div> </div> <div class="col-xl-3 col-md-6 col-xs-12 p-0 text-white bt-inter_regular"> <div> <div id="block-stayconnectedblock" class="block_content:3f9f347f-6b53-40fd-8dfa-25f57854770b"> <div><h4>Stay Connected</h4> <section class="social-icons mb-3"> <a href="https://www.linkedin.com/company/bio-techne" class="text-white mr-2" aria-label="Follow Bio-techne on LinkedIn"><span class="icon-linkedin rounded-pill p-3"></span></a> <a href="https://www.instagram.com/bio_techne/" class="text-white mr-2" aria-label="Follow Bio-techne on Instagram"><span class="icon-instagram rounded-pill p-3"></span></a> <a href="https://twitter.com/biotechne" class="text-white mr-2" aria-label="Follow Bio-techne on X"><span class="icon-x rounded-pill p-3"></span></a> <a href="https://www.youtube.com/channel/UCCE68f793S1eyQM2E1BH0Bw?reload=9" class="text-white mr-2" aria-label="Bio-techne YouTube channel"><span class="icon-youtube rounded-pill p-3"></span></a> <a href="https://www.facebook.com/BioTechne/" class="text-white" aria-label="Follow Bio-techne on Facebook"><span class="icon-facebook rounded-pill p-3"></span></a> </section></div> </div> </div> <div> <a href="/support/contact-us" class="py-2 mt-3 mb-4 text-center font-weight-bold text-decoration-none text-white">Contact Us </a> </div> </div> </div> </div> </footer> </div> <hr> <div class="pb-5 mt-4 d-flex flex-wrap legal-navigation"> <div class="mb-2"> <a href="https://www.bio-techne.com/privacy-and-cookie-policy" class="mr-4 text-white text-center">Privacy and Cookie Policy</a> </div> <div class="mb-2"> <a href="/sitemap" class="mr-4 text-white">Site Map</a> </div> <div class="text-white">© Copyright 2024 Bio-Techne. All Rights Reserved.</div> </div> </div> </div> </div> </div> <div> <div id="block-cookieblock" class="cookie_block"> <div class="cookie-notification-block" data-expires="100"> <div class="container"> <div class="row pt-4 pb-4 pl-5 pr-5"> <div class="col-lg-10 mb-1"> Bio-Techne uses cookies to provide you with a great website experience. By continuing to use this website you acknowledge this and agree to our cookie policy. <a class="cookie-notification-block__link text-white" href="/privacy-and-cookie-policy">Learn more</a>. </div> <div class="col-lg-2 text-lg-right text-center align-middle"> <a href="#" class="btn btn-primary text-nowrap js-cookies"> I Agree </a> </div> </div> </div> </div> </div> </div> </div> <div class="embeddedServiceInvitation" id="snapins_invite" aria-live="assertive" role="dialog" aria-atomic="true" style="display: none;"> <div class="embeddedServiceInvitationHeader" aria-labelledby="snapins_titletext" aria-describedby="snapins_bodytext"> <img id="embeddedServiceAvatar"/> <span class="embeddedServiceTitleText" id="snapins_titletext">Need help?</span> <button type="button" id="closeInvite" class="embeddedServiceCloseIcon" aria-label="Exit invitation">×</button> </div> <div class="embeddedServiceInvitationBody"> <p id="snapins_bodytext">How can we help you?</p> </div> <div class="embeddedServiceInvitationFooter" aria-describedby="snapins_bodytext"> <button type="button" class="embeddedServiceActionButton" id="rejectInvite">Close</button> <button type="button" class="embeddedServiceActionButton" id="acceptInvite">Start Chat</button> </div> </div> <script>window.dataLayer = window.dataLayer || []; window.dataLayer.push({"drupalLanguage":"en","drupalCountry":"US","siteName":"Bio-Techne","brand":"R\u0026D","entityLangcode":"und","entityStatus":"1","entityType":"node","entityBundle":"page","entityId":"10071","entityTitle":"Mass Cytometry (CyTOF)","entityTaxonomy":{"application":{"1036":"CyTOF"},"product_category":{"121":"Antibodies"},"brand":{"327":"Novus Biologicals"}},"userUid":0});</script> <script src="/sites/default/files/js/js_25fMqnS_SV9QKFCPxa9BJeVftykwhKcx00psCZOkjfs.js?scope=footer&delta=0&language=en&theme=bio_techne_global&include=eJxtkW2SwyAIhi8k65EcVKpujWSQZJuefm3TZLo7_QU8MLx8-MJKITdytazkMFFT6w8IDwhPOFwvKJuJqFhxI7GeMq6FpRuq2LUEN6Nmt3QShyHw0rTblwNCukiDeTQbrdrVDBH3kk6VPVbrmbWr4OxCbB_yu4Gu22iQTGJOlZxiskmnvyGm__EXfuPNeHXUMrZA0VHgaSIJZL3CQeGk0IvST4kEtI79uznLz4p9oJHgayHXWMulBNTCze4M3hn4ymHsXZknIQzZzeVG1Z4HhXnpmeTD5hPFgjBuOI3HwDqGYjMLxyWo64QSst2N8yimNCVpT02s5X4MNL4gG3SqFHSfBeaKgTLX-FG14n2DyhjN86WlXVimvdsDwBs4nvIL0JnnFQ"></script> <script src="//resources.bio-techne.com/bio-techne-assets/css/bootstrap/js/bootstrap.bundle.min.js"></script> <script src="/sites/default/files/js/js_Y7jSJq-M3rJaHtFC8IxmKpPWDHPmB6jtjmwzMsh7m6M.js?scope=footer&delta=2&language=en&theme=bio_techne_global&include=eJxtkW2SwyAIhi8k65EcVKpujWSQZJuefm3TZLo7_QU8MLx8-MJKITdytazkMFFT6w8IDwhPOFwvKJuJqFhxI7GeMq6FpRuq2LUEN6Nmt3QShyHw0rTblwNCukiDeTQbrdrVDBH3kk6VPVbrmbWr4OxCbB_yu4Gu22iQTGJOlZxiskmnvyGm__EXfuPNeHXUMrZA0VHgaSIJZL3CQeGk0IvST4kEtI79uznLz4p9oJHgayHXWMulBNTCze4M3hn4ymHsXZknIQzZzeVG1Z4HhXnpmeTD5hPFgjBuOI3HwDqGYjMLxyWo64QSst2N8yimNCVpT02s5X4MNL4gG3SqFHSfBeaKgTLX-FG14n2DyhjN86WlXVimvdsDwBs4nvIL0JnnFQ"></script> </body> </html>

CINXE.COM

Mass cytometry/CyTOF Antibodies