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<!DOCTYPE html> <html lang="en" class="no-js"> <head> <meta charset="UTF-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="applicable-device" content="pc,mobile"> <meta name="viewport" content="width=device-width, initial-scale=1"> <meta name="robots" content="max-image-preview:large"> <meta name="access" content="No"> <meta name="360-site-verification" content="1268d79b5e96aecf3ff2a7dac04ad990" /> <title>The beautiful cell: high-content screening in drug discovery | Analytical and Bioanalytical Chemistry</title> <meta name="twitter:site" content="@SpringerLink"/> <meta name="twitter:card" content="summary_large_image"/> <meta name="twitter:image:alt" content="Content cover image"/> <meta name="twitter:title" content="The beautiful cell: high-content screening in drug discovery"/> <meta name="twitter:description" content="Analytical and Bioanalytical Chemistry - The term “high-content screening” has become synonymous with imaging screens using automated microscopes and automated image analysis. The term..."/> <meta name="twitter:image" content="https://media.springernature.com/full/springer-static/cover-hires/journal/216"/> <meta name="journal_id" content="216"/> <meta name="dc.title" content="The beautiful cell: high-content screening in drug discovery"/> <meta name="dc.source" content="Analytical and Bioanalytical Chemistry 2010 398:1"/> <meta name="dc.format" content="text/html"/> <meta name="dc.publisher" content="Springer"/> <meta name="dc.date" content="2010-06-25"/> <meta name="dc.type" content="ReviewPaper"/> <meta name="dc.language" content="En"/> <meta name="dc.copyright" content="2010 Springer-Verlag"/> <meta name="dc.rights" content="2010 Springer-Verlag"/> <meta name="dc.rightsAgent" content="journalpermissions@springernature.com"/> <meta name="dc.description" content="The term “high-content screening” has become synonymous with imaging screens using automated microscopes and automated image analysis. The term was coined a little over 10 years ago. Since then the technology has evolved considerably and has established itself firmly in the drug discovery and development industry. Both the instruments and the software controlling the instruments and analyzing the data have come to maturity, so the full benefits of high-content screening can now be realized. Those benefits are the capability of carrying out phenotypic multiparametric cellular assays in an unbiased, fully automated, and quantitative fashion. Automated microscopes and automated image analysis are being applied at all stages of the drug discovery and development pipeline. All major pharmaceutical companies have adopted the technology and it is in the process of being embraced broadly by the academic community. This review aims at describing the current capabilities and limits of the technology as well as highlighting necessary developments that are required to exploit fully the potential of high-content screening and analysis."/> <meta name="prism.issn" content="1618-2650"/> <meta name="prism.publicationName" content="Analytical and Bioanalytical Chemistry"/> <meta name="prism.publicationDate" content="2010-06-25"/> <meta name="prism.volume" content="398"/> <meta name="prism.number" content="1"/> <meta name="prism.section" content="ReviewPaper"/> <meta name="prism.startingPage" content="219"/> <meta name="prism.endingPage" content="226"/> <meta name="prism.copyright" content="2010 Springer-Verlag"/> <meta name="prism.rightsAgent" content="journalpermissions@springernature.com"/> <meta name="prism.url" content="https://link.springer.com/article/10.1007/s00216-010-3788-3"/> <meta name="prism.doi" content="doi:10.1007/s00216-010-3788-3"/> <meta name="citation_pdf_url" content="https://link.springer.com/content/pdf/10.1007/s00216-010-3788-3.pdf"/> <meta name="citation_fulltext_html_url" content="https://link.springer.com/article/10.1007/s00216-010-3788-3"/> <meta name="citation_journal_title" content="Analytical and Bioanalytical Chemistry"/> <meta name="citation_journal_abbrev" content="Anal Bioanal Chem"/> <meta name="citation_publisher" content="Springer-Verlag"/> <meta name="citation_issn" content="1618-2650"/> <meta name="citation_title" content="The beautiful cell: high-content screening in drug discovery"/> <meta name="citation_volume" content="398"/> <meta name="citation_issue" content="1"/> <meta name="citation_publication_date" content="2010/09"/> <meta name="citation_online_date" content="2010/06/25"/> <meta name="citation_firstpage" content="219"/> <meta name="citation_lastpage" content="226"/> <meta name="citation_article_type" content="Review"/> <meta name="citation_language" content="en"/> <meta name="dc.identifier" content="doi:10.1007/s00216-010-3788-3"/> <meta name="DOI" content="10.1007/s00216-010-3788-3"/> <meta name="size" content="187191"/> <meta name="citation_doi" content="10.1007/s00216-010-3788-3"/> <meta name="citation_springer_api_url" content="http://api.springer.com/xmldata/jats?q=doi:10.1007/s00216-010-3788-3&api_key="/> <meta name="description" content="The term “high-content screening” has become synonymous with imaging screens using automated microscopes and automated image analysis. The term"/> <meta name="dc.creator" content="Bickle, Marc"/> <meta name="dc.subject" content="Analytical Chemistry"/> <meta name="dc.subject" content="Biochemistry, general"/> <meta name="dc.subject" content="Laboratory Medicine"/> <meta name="dc.subject" content="Characterization and Evaluation of Materials"/> <meta name="dc.subject" content="Food Science"/> <meta name="dc.subject" content="Monitoring/Environmental Analysis"/> <meta name="citation_reference" content="citation_journal_title=Health Econ; citation_author=CP Adams, VV Brantner; citation_volume=19; citation_publication_date=2010; citation_pages=130-141; citation_doi=10.1002/hec.1454; citation_id=CR1"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_author=H Pearson; citation_volume=444; citation_publication_date=2006; citation_pages=532-533; citation_doi=10.1038/444532a; citation_id=CR2"/> <meta name="citation_reference" content="citation_journal_title=Nat Rev Drug Discov; citation_author=B Hughes; 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The term was coined a little over 10 years ago. Since then the technology has evolved considerably and has established itself firmly in the drug discovery and development industry. Both the instruments and the software controlling the instruments and analyzing the data have come to maturity, so the full benefits of high-content screening can now be realized. Those benefits are the capability of carrying out phenotypic multiparametric cellular assays in an unbiased, fully automated, and quantitative fashion. Automated microscopes and automated image analysis are being applied at all stages of the drug discovery and development pipeline. All major pharmaceutical companies have adopted the technology and it is in the process of being embraced broadly by the academic community. This review aims at describing the current capabilities and limits of the technology as well as highlighting necessary developments that are required to exploit fully the potential of high-content screening and analysis."/> <meta property="og:image" content="https://media.springernature.com/full/springer-static/cover-hires/journal/216"/> <meta name="format-detection" content="telephone=no"> <link rel="apple-touch-icon" sizes="180x180" href=/oscar-static/img/favicons/darwin/apple-touch-icon-92e819bf8a.png> <link rel="icon" type="image/png" sizes="192x192" href=/oscar-static/img/favicons/darwin/android-chrome-192x192-6f081ca7e5.png> <link rel="icon" type="image/png" sizes="32x32" href=/oscar-static/img/favicons/darwin/favicon-32x32-1435da3e82.png> <link rel="icon" type="image/png" sizes="16x16" href=/oscar-static/img/favicons/darwin/favicon-16x16-ed57f42bd2.png> <link rel="shortcut icon" data-test="shortcut-icon" href=/oscar-static/img/favicons/darwin/favicon-c6d59aafac.ico> <meta 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The term was coined a little over 10 years ago. Since then the technology has evolved considerably and has established itself firmly in the drug discovery and development industry. Both the instruments and the software controlling the instruments and analyzing the data have come to maturity, so the full benefits of high-content screening can now be realized. Those benefits are the capability of carrying out phenotypic multiparametric cellular assays in an unbiased, fully automated, and quantitative fashion. Automated microscopes and automated image analysis are being applied at all stages of the drug discovery and development pipeline. All major pharmaceutical companies have adopted the technology and it is in the process of being embraced broadly by the academic community. This review aims at describing the current capabilities and limits of the technology as well as highlighting necessary developments that are required to exploit fully the potential of high-content screening and analysis.","datePublished":"2010-06-25T00:00:00Z","dateModified":"2010-06-25T00:00:00Z","pageStart":"219","pageEnd":"226","sameAs":"https://doi.org/10.1007/s00216-010-3788-3","keywords":["High-content screening","Imaging","Drug discovery","Multiparametric","Cell-based assay","Analytical Chemistry","Biochemistry","general","Laboratory Medicine","Characterization and Evaluation of Materials","Food Science","Monitoring/Environmental Analysis"],"image":[],"isPartOf":{"name":"Analytical and Bioanalytical Chemistry","issn":["1618-2650","1618-2642"],"volumeNumber":"398","@type":["Periodical","PublicationVolume"]},"publisher":{"name":"Springer-Verlag","logo":{"url":"https://www.springernature.com/app-sn/public/images/logo-springernature.png","@type":"ImageObject"},"@type":"Organization"},"author":[{"name":"Marc Bickle","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","address":{"name":"Technology Development Studio, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany","@type":"PostalAddress"},"@type":"Organization"}],"email":"bickle@mpi-cbg.de","@type":"Person"}],"isAccessibleForFree":false,"hasPart":{"isAccessibleForFree":false,"cssSelector":".main-content","@type":"WebPageElement"},"@type":"ScholarlyArticle"},"@context":"https://schema.org","@type":"WebPage"}</script> </head> <body class="" >  <noscript> <iframe src="https://www.googletagmanager.com/ns.html?id=GTM-MRVXSHQ" height="0" width="0" 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</li> <li class="c-breadcrumbs__item" id="breadcrumb2" itemprop="itemListElement" itemscope="" itemtype="https://schema.org/ListItem"> <span itemprop="name">Article</span><meta itemprop="position" content="3"> </li> </ol> </nav> <h1 class="c-article-title" data-test="article-title" data-article-title="">The beautiful cell: high-content screening in drug discovery</h1> <ul class="c-article-identifiers"> <li class="c-article-identifiers__item" data-test="article-category">Review</li> <li class="c-article-identifiers__item"> Published: <time datetime="2010-06-25">25 June 2010</time> </li> </ul> <ul class="c-article-identifiers c-article-identifiers--cite-list"> <li class="c-article-identifiers__item"> <span data-test="journal-volume">Volume 398</span>, pages 219–226, (<span data-test="article-publication-year">2010</span>) </li> <li class="c-article-identifiers__item c-article-identifiers__item--cite"> <a href="#citeas" data-track="click" data-track-action="cite this article" 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class="app-article-masthead__journal-title">Analytical and Bioanalytical Chemistry</span> </a> <a href="https://link.springer.com/journal/216/aims-and-scope" class="app-article-masthead__submission-link" data-track="click_aims_and_scope" data-track-action="aims and scope" data-track-context="article page" data-track-label="link"> Aims and scope <svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-arrow-right-medium"></use></svg> </a> <a href="https://mc.manuscriptcentral.com/abc" class="app-article-masthead__submission-link" data-track="click_submit_manuscript" data-track-context="article masthead on springerlink article page" data-track-action="submit manuscript" data-track-label="link"> Submit manuscript <svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-arrow-right-medium"></use></svg> </a> </div> </div> </div> </section> <div class="c-article-main u-container u-mt-24 u-mb-32 l-with-sidebar" id="main-content" data-component="article-container"> <main class="u-serif js-main-column" data-track-component="article body"> <div class="c-article-header"> <header> <ul class="c-article-author-list c-article-author-list--short" data-test="authors-list" data-component-authors-activator="authors-list"><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Marc-Bickle-Aff1" data-author-popup="auth-Marc-Bickle-Aff1" data-author-search="Bickle, Marc" data-corresp-id="c1">Marc Bickle<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-mail-medium"></use></svg></a><sup class="u-js-hide"><a href="#Aff1">1</a></sup> 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data-track-label="link" rel="nofollow">Explore all metrics <svg class="u-icon app-article-metrics-bar__arrow-icon" width="24" height="24" aria-hidden="true" focusable="false"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></a></p> </li> </ul> </div> <div class="u-mt-32"> </div> </header> </div> <div data-article-body="true" data-track-component="article body" class="c-article-body"> <section aria-labelledby="Abs1" data-title="Abstract" lang="en"><div class="c-article-section" id="Abs1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Abs1">Abstract</h2><div class="c-article-section__content" id="Abs1-content"><p>The term “high-content screening” has become synonymous with imaging screens using automated microscopes and automated image analysis. The term was coined a little over 10 years ago. Since then the technology has evolved considerably and has established itself firmly in the drug discovery and development industry. Both the instruments and the software controlling the instruments and analyzing the data have come to maturity, so the full benefits of high-content screening can now be realized. Those benefits are the capability of carrying out phenotypic multiparametric cellular assays in an unbiased, fully automated, and quantitative fashion. Automated microscopes and automated image analysis are being applied at all stages of the drug discovery and development pipeline. All major pharmaceutical companies have adopted the technology and it is in the process of being embraced broadly by the academic community. 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Open Source Tools for High Content Screening </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Chapter</span> <span class="c-article-meta-recommendations__date">© 2013</span> </div> </div> </article> </div> </div> </section> <script> window.dataLayer = window.dataLayer || []; window.dataLayer.push({ recommendations: { recommender: 'semantic', model: 'specter', policy_id: 'NA', timestamp: 1732357811, embedded_user: 'null' } }); </script> <div id="MagazineFulltextArticleBodySuffix"><section aria-labelledby="Bib1" data-title="References"><div class="c-article-section" id="Bib1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Bib1">References</h2><div class="c-article-section__content" id="Bib1-content"><div data-container-section="references"><ol class="c-article-references" data-track-component="outbound reference" 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The beautiful cell: high-content screening in drug discovery. <i>Anal Bioanal Chem</i> <b>398</b>, 219–226 (2010). https://doi.org/10.1007/s00216-010-3788-3</p><p class="c-bibliographic-information__download-citation u-hide-print"><a data-test="citation-link" data-track="click" data-track-action="download article citation" data-track-label="link" data-track-external="" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/s00216-010-3788-3?format=refman&flavour=citation">Download citation<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></p><ul class="c-bibliographic-information__list" data-test="publication-history"><li class="c-bibliographic-information__list-item"><p>Received<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2010-02-28">28 February 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