Fission Yeast: A Laboratory Manual

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border="0"></a></p> </td> <td colspan=3 rowspan="2" valign="top" > <h4 style="color:black;">Fission Yeast: A Laboratory Manual</h4> <br><font face="[Font[__TEXT_FONT_INDEX]]" size="-1"><font color="#777777"><b>Subject Area(s):</b></font>  <A HREF="default.tpl?cart=17323931832305193620&id1=108&startat=1&--woSECTIONSdatarq=108&--SECTIONSword=ww&--Publication_Yearsort=1&--Sequence_numbersort=2&--Publication_yearsdir=de&--Sequence_numbersdir=de">Yeast</A>; <A HREF="default.tpl?cart=17323931832305193620&id1=24&startat=1&--woSECTIONSdatarq=24&--SECTIONSword=ww&--Publication_Yearsort=1&--Sequence_numbersort=2&--Publication_yearsdir=de&--Sequence_numbersdir=de">Cell Biology</A>; <A HREF="default.tpl?cart=17323931832305193620&id1=101&startat=1&--woSECTIONSdatarq=101&--SECTIONSword=ww&--Publication_Yearsort=1&--Sequence_numbersort=2&--Publication_yearsdir=de&--Sequence_numbersdir=de">Microbiology</A><br> <br><font face="[Font[__TEXT_FONT_INDEX]]" size=-1>Edited by Iain Hagan, <font face="[Font[__TEXT_FONT_INDEX]]" size=-1><em>Cancer Research UK Manchester Institute, University of Manchester; </em>Antony M. Carr, <font face="[Font[__TEXT_FONT_INDEX]]" size=-1><em>University of Sussex; </em>Agnes Grallert, <font face="[Font[__TEXT_FONT_INDEX]]" size=-1><em>Cancer Research UK Manchester Institute, University of Manchester; </em>Paul Nurse, <font face="[Font[__TEXT_FONT_INDEX]]" size=-1><em>The Francis Crick Institute</em> <p><font face="[Font[__TEXT_FONT_INDEX]]" size="-1"><strong><p style="margin-bottom:.3em;">Download a Free Excerpt from <em>Fission Yeast</em>:</p> <p style="margin-top:.3em; font-weight:normal;"><a href="pdf/sample/2016/fissionyeast/FissionYstFM.pdf" target="_blank">Preface</a><br> <a href="pdf/sample/2016/fissionyeast/FissionYstIntro.pdf" target="_blank">Intro: Introduction to Fission Yeast as a Model System<br> <a href="pdf/sample/2016/fissionyeast/FissionYstChap2.pdf" target="_blank">Chapter 2: Growth and the Environment of <em>Schizosaccharomyces pombe</em><br> <a href="pdf/sample/2016/fissionyeast/FissionYstProt.pdf" target="_blank">Protocol: Mating, Spore Dissection, and Selection of Diploid Cells in <em>Schizosaccharomyces japonicus</em><br> <a href="pdf/sample/2016/fissionyeast/FissionYstIndex.pdf" target="_blank">Index</a></p></strong></p> </td> </tr> <tr> </tr> <tr>  <td colspan="4" valign="top"> <br> <font face="[Font[__TEXT_FONT_INDEX]]" size="-1"><p>© 2016  490 pages, illustrated (32 color, 17 B&W), index<br> <font face="[Font[__TEXT_FONT_INDEX]]" size="-1"> <font face="[Font[__TEXT_FONT_INDEX]]" size="-1">Paperback  <font face="[Font[__TEXT_FONT_INDEX]]" size="-1">    <strong><strike>$90</strike></strong> <strong><font color=red>72.00</font></strong> <br>ISBN  <font face="[Font[__TEXT_FONT_INDEX]]" size="-1">978-1-621820-82-6 <br>You save: <span style="color:red;font-weight:bold;">20%</span> <br>You will receive <span style="color:red;font-weight:bold;">free shipping</span> on this item at checkout. <br><span style="font-size:85%;">Free shipping offer applies to direct website purchases by individual U.S. customers only.</span> <br> <br>  <p><b>This title also available in: <a href="default.tpl?cart=17323931832305193620&action=full&--eqskudatarq=1105">Hardcover</a></p> <input type="hidden" name="the_price" value="72"> </form> </p>  </td> </tr> <tr> <td></td><td></td><td colspan=4> </td></tr> <tr><td colspan=6 align=left><font face="[Font[__TEXT_FONT_INDEX]]" size="-1"> <div class="section_tabs"> <ul class="tabs_bk">  <li class="current">     Description     </li> <li>     Contents     </li> <li>     Related Titles     </li> </ul> <div class="box visible"> <A name="description"><h4>Description</h4></a> <p>Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies.</p> <p>This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories.</p> <p>The well-studied fission yeast <em>Schizosaccharomyces pombe</em> is the focus throughout, but the emerging model <em>S. japonicus</em>a larger, dimorphic species with several desirable characteristicsis also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.</p> </div> <div class="box"><h4>Contents</h4></A> <dl> <dt style="padding-top:6px;">Preface</dt> <dt style="padding-top:12px;">CHAPTER 1 INTRODUCTION</dt> <dt style="padding-top:6px;">Introduction to Fission Yeast as a Model System</dt> <dd><em>Jacqueline Hayles and Paul Nurse</em></dd> <dt style="padding-top:12px;">CHAPTER 2 INTRODUCTION</dt> <dt style="padding-top:6px;"> Growth and the Environment of <em>Schizosaccharomyces pombe</em></dt> <dd><em>Janni Petersen and Paul Russell</em></dd> <dt style="padding-top:12px;">CHAPTER 3 INTRODUCTION</dt> <dt style="padding-top:6px;">Genetic Analysis of <em>Schizosaccharomyces pombe</em></dt> <dd><em>Karl Ekwall and Geneviève Thon</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Setting Up <em>Schizosaccharomyces pombe</em> Crosses/Matings</dt> <dd><em>Karl Ekwall and Geneviève Thon</em></dd> <dt style="padding-top:6px;">2 Selecting <em>Schizosaccharomyces pombe</em> Diploids</dt> <dd><em>Karl Ekwall and Geneviève Thon</em></dd> <dt style="padding-top:6px;">3 Spore Analysis and Tetrad Dissection of <em>Schizosaccharomyces pombe</em></dt> <dd><em>Karl Ekwall and Geneviève Thon</em></dd> <dt style="padding-top:6px;">4 Mating-Type Determination in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Karl Ekwall and Geneviève Thon</em></dd> <dt style="padding-top:6px;">5 Ethyl Methanesulfonate Mutagenesis in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Karl Ekwall and Geneviève Thon</em></dd> <dt style="padding-top:12px;">CHAPTER 4 INTRODUCTION</dt> <dt style="padding-top:6px;">Molecular Genetic Tools and Techniques in Fission Yeast</dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Transformation of <em>Schizosaccharomyces pombe</em>: Electroporation Procedure</dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:6px;">2 Transformation of <em>Schizosaccharomyces pombe</em>: Protoplast Procedure</dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:6px;">3 Identifying Products of Recombinase-Mediated Cassette Exchange (RMCE) in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:6px;">4 Transformation of <em>Schizosaccharomyces pombe</em>: Lithium Acetate/ Dimethyl Sulfoxide Procedure</dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:6px;">5 Extraction of Chromosomal DNA from <em>Schizosaccharomyces pombe</em></dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:6px;">6 Colony Polymerase Chain Reaction with <em>Schizosaccharomyces pombe</em></dt> <dd><em>Johanne M. Murray, Adam T. Watson, and Antony M. Carr</em></dd> <dt style="padding-top:12px;">CHAPTER 5 INTRODUCTION</dt> <dt style="padding-top:6px;">Analysis of RNA Metabolism in Fission Yeast</dt> <dd><em>Jo Ann Wise and Olaf Nielsen</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Preparation of Total RNA from Fission Yeast</dt> <dd><em>Jürg Bähler and Jo Ann Wise</em></dd> <dt style="padding-top:6px;">2 <em>Schizosaccharomyces pombe</em> Polysome Profile Analysis and RNA Purification</dt> <dd><em>Dieter A. Wolf, Jürg Bähler, and Jo Ann Wise</em></dd> <dt style="padding-top:6px;">3 4-Thiouridine Labeling to Analyze mRNA Turnover in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Juan Mata and Jo Ann Wise</em></dd> <dt style="padding-top:12px;">CHAPTER 6 INTRODUCTION</dt> <dt style="padding-top:6px;">Elementary Protein Analysis in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Agnes Grallert and Iain M. Hagan</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Preparation of Protein Extracts from <em>Schizosaccharomyces pombe</em> Using Trichloroacetic Acid Precipitation</dt> <dd><em>Agnes Grallert and Iain M. Hagan</em></dd> <dt style="padding-top:6px;">2 Small-Scale Immunoprecipitation from Fission Yeast Cell Extracts</dt> <dd><em>Agnes Grallert and Iain M. Hagan</em></dd> <dt style="padding-top:6px;">3 Large-Scale Immunoprecipitation from Fission Yeast Cell Extracts</dt> <dd><em>Agnes Grallert and Iain M. Hagan</em></dd> <dt style="padding-top:6px;">4 Large-Scale Purification of Small Ubiquitin-Like Modifier (SUMO)-Modified Proteins from <em>Schizosaccharomyces pombe</em></dt> <dd><em>Minghua Nie and Michael N. Boddy</em></dd> <dt style="padding-top:6px;">5 Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from <em>Schizosaccharomyces pombe</em></dt> <dd><em>Nicola Zilio and Michael N. Boddy</em></dd> <dt style="padding-top:12px;">CHAPTER 7 INTRODUCTION</dt> <dt style="padding-top:6px;">Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technology in Fission Yeast</dt> <dd><em>Boris Maček, Alejandro Carpy, André Koch, Claudia C. Bicho,  Weronika E. Borek, Silke Hauf, and Kenneth E. Sawin</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Strains</dt> <dd><em>André Koch, Claudia C. Bicho, Weronika E. Borek, Alejandro Carpy, Boris Maček, Silke Hauf, and Kenneth E. Sawin</em></dd> <dt style="padding-top:6px;">2 Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast</dt> <dd><em>Alejandro Carpy, André Koch, Claudia C. Bicho, Weronika E. Borek, Silke Hauf, Kenneth E. Sawin, and Boris Maček</em></dd> <dt style="padding-top:12px;">CHAPTER 8 INTRODUCTION</dt> <dt style="padding-top:6px;">Electron Microscopy of Fission Yeast</dt> <dd><em>J. Richard McIntosh, Mary K. Morphew, and Thomas H. Giddings, Jr.</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Preparing Fission Yeast for Electron Microscopy</dt> <dd><em>Thomas H. Giddings, Jr., Mary K. Morphew, and J. Richard McIntosh</em></dd> <dt style="padding-top:6px;">2 Immunolocalization of Proteins in Fission Yeast by Electron Microscopy</dt> <dd><em>Mary K. Morphew, Thomas H. Giddings, Jr., and J. Richard McIntosh</em></dd> <dt style="padding-top:6px;">3 Cryoelectron Microscopy of Fission Yeast</dt> <dd><em>Mary K. Morphew, Thomas H. Giddings, Jr., and J. Richard McIntosh</em></dd> <dt style="padding-top:12px;">CHAPTER 9 INTRODUCTION</dt> <dt style="padding-top:6px;">Fixed-Cell Imaging of <em>Schizosaccharomyces pombe</em></dt> <dd><em>Iain M. Hagan and Steven Bagley</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Immunofluorescence Microscopy of <em>Schizosaccharomyces pombe</em> Using Chemical Fixation</dt> <dd><em>lain M. Hagan</em></dd> <dt style="padding-top:6px;">2 Chromatin and Cell Wall Staining of <em>Schizosaccharomyces pombe</em></dt> <dd><em>lain M. Hagan</em></dd> <dt style="padding-top:6px;">3 Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates</dt> <dd><em>lain M. Hagan</em></dd> <dt style="padding-top:12px;">CHAPTER 10 INTRODUCTION</dt> <dt style="padding-top:6px;"> Live Cell Imaging in Fission Yeast</dt> <dd><em>Daniel P. Mulvihill</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Microscopic Observation of Living Cells Stained with Fluorescent Probes</dt> <dd><em>Haruhiko Asakawa, Da-Qiao Ding, Tokuko Haraguchi, and  Yasushi Hiraoka</em></dd> <dt style="padding-top:6px;">2 Visualization of a Specific Genome Locus by the lacO/Lacl-GFP System</dt> <dd><em>Da-Qiao Ding and Yasushi Hiraoka</em></dd> <dt style="padding-top:6px;">3 Live Cell Imaging of the <em>Schizosaccharomyces pombe</em> Sexual Life Cycle</dt> <dd><em>Laura Merlini, Aleksandar Vjestica, Omaya Dudin, Felipe Bendezú, and Sophie G. Martin</em></dd> <dt style="padding-top:12px;">CHAPTER 11 INTRODUCTION</dt> <dt style="padding-top:6px;">Analysis of <em>Schizosaccharomyces pombe</em> Meiosis</dt> <dd><em>Akira Yamashita, Takeshi Sakuno, Yoshinori Watanabe, and  Masayuki Yamamoto</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Live Imaging of Chromosome Segregation during Meiosis in the Fission Yeast <em>Schizosaccharomyces pombe</em></dt> <dd><em>Akira Yamashita, Takeshi Sakuno, Yoshinori Watanabe, and Masayuki Yamamoto</em></dd> <dt style="padding-top:6px;">2 Synchronous Induction of Meiosis in the Fission Yeast <em>Schizosaccharomyces pombe</em></dt> <dd><em>Akira Yamashita, Takeshi Sakuno, Yoshinori Watanabe, and  Masayuki Yamamoto</em></dd> <dt style="padding-top:6px;">3 A Simple Method to Induce Meiosis and Sporulation Semisynchronously in the Fission Yeast <em>Schizosaccharomyces pombe</em></dt> <dd><em>Akira Yamashita, Takeshi Sakuno, Yoshinori Watanabe, and  Masayuki Yamamoto</em></dd> <dt style="padding-top:12px;">CHAPTER 12 INTRODUCTION</dt> <dt style="padding-top:6px;">Analysis of the <em>Schizosaccharomyces pombe</em> Cell Cycle</dt> <dd><em>lain M. Hagan, Agnes Grallert, and Viesturs Simanis</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Cell Cycle Synchronization of <em>Schizosaccharomyces pombe</em> by Centrifugal Elutriation of Small Cells</dt> <dd><em>lain M. Hagan, Agnes Grallert, and Viesturs Simanis</em></dd> <dt style="padding-top:6px;">2 Cell Cycle Synchronization of <em>Schizosaccharomyces pombe</em> by Lactose Gradient Centrifugation to Isolate Small Cells</dt> <dd><em>lain M. Hagan, Agnes Grallert, and Viesturs Simanis</em></dd> <dt style="padding-top:6px;">3 Synchronizing Progression of <em>Schizosaccharomyces pombe</em> Cells from Prophase through Mitosis and into S Phase with nda3-KM311 Arrest Release</dt> <dd><em>lain M. Hagan, Agnes Grallert, and Viesturs Simanis</em></dd> <dt style="padding-top:6px;">4 Synchronizing Progression of <em>Schizosaccharomyces pombe</em> Cells from G2 through Repeated Rounds of Mitosis and S Phase with cdc25-22 Arrest Release</dt> <dd><em>lain M. Hagan, Agnes Grallert, and Viesturs Simanis</em></dd> <dt style="padding-top:6px;">5 Synchronization of S Phase in <em>Schizosaccharomyces pombe</em> Cells by Transient Exposure to M-Factor Pheromone</dt> <dd><em>Olaf Nielsen</em></dd> <dt style="padding-top:6px;">6 Analyzing <em>Schizosaccharomyces pombe</em> DNA Content by Flow Cytometry</dt> <dd><em>Eric Boye, Silje Anda, Christiane Rothe, Trond Stokke, and Beata Grallert</em></dd> <dt style="padding-top:12px;">CHAPTER 13 INTRODUCTION</dt> <dt style="padding-top:6px;">Analysis of DNA Metabolism in Fission Yeast</dt> <dd><em>Francisco Antequera and Timothy C. Humphrey</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Determination of the Frequency of Minichromosome Loss to Assess Chromosomal Stability in Fission Yeast</dt> <dd><em>Osami Niwa</em></dd> <dt style="padding-top:6px;">2 Two-Dimensional Gel Electrophoresis of DNA Replication Intermediates in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Mar Sánchez and Francisco Antequera</em></dd> <dt style="padding-top:6px;">3 Analysis of DNA Replication in Fission Yeast by Combing</dt> <dd><em>Divya R. lyer, Shankar Das, and Nick Rhind</em></dd> <dt style="padding-top:6px;">4 Using Pulsed-Field Gel Electrophoresis to Analyze <em>Schizosaccharomyces pombe</em> Chromosomes and Chromosomal Elements</dt> <dd><em>Chen-Chun Pai, Carol Walker, and Timothy C. Humphrey</em></dd> <dt style="padding-top:6px;">5 DNA Double-Strand Break Repair Assay</dt> <dd><em>Chen-Chun Pai, Elizabeth Blaikley, and Timothy C. Humphrey</em></dd> <dt style="padding-top:12px;">CHAPTER 14 INTRODUCTION</dt> <dt style="padding-top:6px;">Analysis of Heterochromatin in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Hugh P. Cam and Simon Whitehall</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Reporter Gene Silencing Assays in Fission Yeast</dt> <dd><em>Hugh P. Cam and Simon Whitehall</em></dd> <dt style="padding-top:6px;">2 Analysis of <em>Schizosaccharomyces pombe</em> Heterochromatin-Associated Short Interfering RNAs</dt> <dd><em>Ke Zhang</em></dd> <dt style="padding-top:6px;">3 Micrococcal Nuclease Digestion of <em>Schizosaccharomyces pombe</em> Chromatin</dt> <dd><em>Hugh P. Cam and Simon Whitehall</em></dd> <dt style="padding-top:6px;">4 Chromatin Immunoprecipitation (ChIP) in <em>Schizosaccharomyces pombe</em></dt> <dd><em>Hugh P. Cam and Simon Whitehall</em></dd> <dt style="padding-top:12px;">CHAPTER 15 INTRODUCTION</dt> <dt style="padding-top:6px;">Fission Yeast Cell Wall Analysis</dt> <dd><em>Pilar Pérez and Juan C. Ribas</em></dd> <dt style="padding-top:9px;">PROTOCOL</dt> <dt style="padding-top:6px;">1 Radioactive Labeling and Fractionation of Fission Yeast Walls</dt> <dd><em>Pilar Pérez and Juan C. Ribas</em></dd> <dt style="padding-top:12px;">CHAPTER 16 INTRODUCTION</dt> <dt style="padding-top:6px;">High-Throughput Quantitative Genetic Interaction Mapping in the Fission Yeast <em>Schizosaccharomyces pombe</em></dt> <dd><em>Assen Roguev, Colm J. Ryan, Edgar Hartsuiker, and Nevan J. Krogan</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Transformation of <em>Schizosaccharomyces pombe</em> in a 96-Well Format</dt> <dd><em>Assen Roguev, Jiewei Xu, and Nevan J. Krogan</em></dd> <dt style="padding-top:6px;">2 DNA Preparation from <em>Schizosaccharomyces pombe</em></dt> <dd><em>Assen Roguev, Jiewei Xu, and Nevan J. Krogan</em></dd> <dt style="padding-top:6px;">3 Genetic Interaction Screens in <em>Schizosaccharomyces pombe</em> Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator</dt> <dd><em>Isabelle Colson and Edgar Hartsuiker</em></dd> <dt style="padding-top:6px;">4 Genetic Interaction Mapping in <em>Schizosaccharomyces pombe</em> Using the Pombe Epistasis Mapper (PEM) System and a ROTOR HDA Colony Replicating Robot in a 1536 Array Format</dt> <dd><em>Assen Roguev, Jiewei Xu, and Nevan J. Krogan</em></dd> <dt style="padding-top:6px;">5 Genetic Interaction Score (S-Score) Calculation, Clustering, and Visualization of Genetic Interaction Profiles for Yeast</dt> <dd><em>Assen Roguev, Colm J. Ryan, Jiewei Xu, Isabelle Colson, Edgar Hartsuiker, and Nevan J. Krogan</em></dd> <dt style="padding-top:12px;">CHAPTER 17 INTRODUCTION</dt> <dt style="padding-top:6px;">Metabolomic Analysis of <em>Schizosaccharomyces pombe</em>: Sample Preparation, Detection, and Data Interpretation</dt> <dd><em>Tomáš Pluskal and Mitsuhiro Yanagida</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Preparation of Intracellular Metabolite Extracts from Liquid <em>Schizosaccharomyces pombe</em> Cultures</dt> <dd><em>Tomáš Pluskal, Takahiro Nakamura, and Mitsuhiro Yanagida</em></dd> <dt style="padding-top:6px;">2 Measurement of Metabolome Samples Using Liquid Chromatography-Mass Spectrometry, Data Acquisition, and Processing</dt> <dd><em>Tomas Pluskal and Mitsuhiro Yanagida</em></dd> <dt style="padding-top:12px;">CHAPTER 18 INTRODUCTION</dt> <dt style="padding-top:6px;"><em>Schizosaccharomyces japonicus</em>: A Distinct Dimorphic Yeast among the Fission Yeast</dt> <dd><em>Keita Aoki, Kanji Furuya, and Hironori Niki</em></dd> <dt style="padding-top:9px;">PROTOCOLS</dt> <dt style="padding-top:6px;">1 Mating, Spore Dissection, and Selection of Diploid Cells in <em>Schizosaccharomyces japonicus</em></dt> <dd><em>Kanji Furuya and Hironori Niki</em></dd> <dt style="padding-top:6px;">2 Transformation of <em>Schizosaccharomyces japonicus</em></dt> <dd><em>Keita Aoki and Hironori Niki</em></dd> <dt style="padding-top:6px;">3 Induction of Hyphal Growth in <em>Schizosaccharomyces japonicus</em></dt> <dd><em>Hironori Niki</em></dd> <dt style="padding-top:12px;">APPENDIX General Safety and Hazardous Material Information</dt> <dt style="padding-top:12px;">INDEX</dt> </dl> </div> <div class="box"><A name="relateds"><h4>Related Titles</h4></A>  <br><font face="[Font[__TEXT_FONT_INDEX]]" size="-1"><a href="default.tpl?action=full&cart=17323931832305193620&--eqskudatarq=1105">Fission Yeast: A Laboratory Manual</a> [<em>Hardcover</em>]<br> </div> </div> <tr> <td colspan="2" align="center"><font face="[Font[__TEXT_FONT_INDEX]]" 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