PhosphoSite

<script src="https://code.jquery.com/jquery-3.6.3.min.js" integrity="sha256-pvPw+upLPUjgMXY0G+8O0xUf+/Im1MZjXxxgOcBQBXU=" crossorigin="anonymous"></script>  <script src="//ajax.googleapis.com/ajax/libs/jqueryui/1.13.2/jquery-ui.min.js"></script>  <script language="JavaScript" type="text/javascript" src="/dist/6.7.5_1/js/min/global.min.js"></script>  <link rel="stylesheet" type="text/css" href="/dist/6.7.5_1/css/min/msdb.min.css">   <!DOCTYPE html>  <style type="text/css"> .headerStyle { background-image: url(//media.cellsignal.com/www/images/project-images/img/assets/background-header@15.png); background-color: #3e7998; background-repeat:no-repeat; background-attachment: fixed; width:100%; } .pspLogoText { padding-left:0px; font-size:30px; color:#ffffff; font-family:PT Serif; font-weight:normal; } html { position: relative; min-height: 100%; } .containerHeader { width:1000px; position:relative; margin:auto; max-width:1000px; border-spacing: 0px; } .containerHeader td { color:#ffffff; } /* Styles for Menu dropdowns in header*/ .clearfix:after { display:block; clear:both; } /*----- Menu Outline -----*/ .menu-wrap { width:100%; box-shadow:0px 1px 3px rgba(0,0,0,0.2); background:#BDBDBD } .menu { vertical-align: middle; /* width:1280px;*/ width:auto; margin:0px auto; } .menu li { margin:0px; list-style:none; font-family:Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; } .menu a { transition:all linear 0.15s; color:#ffffff; } .menu li:hover > a, .menu .current-item > a { text-decoration:none; color:#ffffff; } .menu .arrow { font-size:16px; line-height:0%; } /*----- Top Level -----*/ .menu > ul > li { float:left; display:inline-block; position:relative; font-size:19px; } .menu > ul > li > a { /* padding:5px 30px; */ /*10px 40px */ padding:5px 30px; display:inline-block; text-shadow:0px 1px 0px rgba(0,0,0,0.4); font-family: Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; font-size: 16px; font-weight: normal; } .menu > ul > li:hover > a, .menu > ul > .current-item > a { background:#BDBDBD; } /*----- Bottom Level -----*/ .menu li:hover .sub-menu { z-index:1; opacity:1; } .sub-menu { width:200%; padding:5px 0px; position:absolute; top:100%; left:0px; z-index:-1; opacity:0; transition:opacity linear 0.15s; box-shadow:0px 2px 3px rgba(0,0,0,0.2); background:#BDBDBD; } .sub-menu li { display:block; font-size:16px; } .sub-menu li a { padding:10px 30px; display:block; font-family: Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; font-size: 16px; font-weight: normal; } .sub-menu li a:hover, .sub-menu .current-item a { background: #3E7998; } .cstPSPText { color:#ffffff;font-family:PT Serif;font-weight:normal;font-size:15px } .cstPSPText:hover{ text-decoration:none; } .titleMainHeaderText { color: #3e7998; font-family: Verdana, Arial, Helvetica, sans-serif; font-weight: bold; font-size: 12px; padding: 10px 10px 10px 20px; } .prodVersion { color:#ffffff;font-family:Verdana, Arial, Helvetica, sans-serif;;font-weight:normal;font-size:12px } </style> <html> <head>  <script>(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-T3KDPT6');</script>  <META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">   <link rel="shortcut icon" type="image/x-icon" href="/dist/6.7.5_1/images/favicon.ico" /> <script type="text/javascript"> function recordOutboundLink(link, category, action) { _gat._getTrackerByName()._trackEvent(category, action, link.href); setTimeout('document.location = "' + link.href + '"', 100); } (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){ (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o), m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m) })(window,document,'script','https://www.google-analytics.com/analytics.js','ga'); ga('create', 'UA-4351229-4', 'auto'); ga('send', 'pageview'); function handleOutboundLinkClicks(event) { //On click event tracking ga('send', 'event', { eventCategory: 'Outbound Link', eventAction: 'click', eventLabel: event.target.href, transport: 'beacon' }); } </script> </head> <title>PhosphoSite</title> <div id="checkJS" class="checkJavascript">Javascript is not enabled on this browser. This site will not work properly without Javascript.</div> <script type="text/javascript"> setStyleValue('checkJS','visibility', 'hidden'); var cookieEnabled = (navigator.cookieEnabled) if (typeof navigator.cookieEnabled == "undefined" && !cookieEnabled) { document.cookie = "testcookie" cookieEnabled = (document.cookie.indexOf("testcookie") != -1) } if (!cookieEnabled) { alert('You must enable cookie support for your browser to use this site.'); putSessionAttribute("cookieEnabled", "no"); } else { putSessionAttribute("cookieEnabled", "yes"); } var clientAddress = '8.222.208.146, 172.70.147.203, 172.20.6.60'; dataLayer = window.datalayer || []; dataLayer.push({ 'pageType': 'PhosphoSite', 'topic' : '', 'clientAddress' : clientAddress == 'null' ?'172.20.6.84' : clientAddress, 'event':'PSP data push' }); </script> <body onload="checkErrors()" style="margin:0;" bgcolor="#FFFFFF">  <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-T3KDPT6" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript>  <div class="headerStyle"> <table class="containerHeader" > <tr> <td width="360px"> <a href="/homeAction" target="_self" style="text-decoration: none;"> <table> <tr> <td rowspan="2"><img src="images/header/logo_psp_white_cp.png" alt="PhosphoSitePlus Homepage" align="center" height="60px"/></td> <td valign="top"><span class="pspLogoText">PhosphoSite<span style="color:#f1b6da">Plus<sup>®</sup> <span class="prodVersion" >v6.7.5</span></span></span> </td> </tr> </table> </a> </td> <td valign="top" align="center"><a href="https://www.cellsignal.com/" class="cstPSPText" target="_blank">Powered by Cell Signaling Technology</a></td> <td style="float:right"> <div class="menu-wrap"> <nav class="menu"> <ul class="clearfix" style="float:right; margin:0px; padding-right:10px; "> <li> <a href="/../staticFeedback.action" style="padding: 5px 5px"><img src="images/header/icon_feedback.png" alt="Feedback" align="center"/> Feedback</a> </li> <li><span style="color:#ffffff;"> | </span></li> <li> <a href="/../staticContact.action" style="padding: 5px 5px"><img src="images/header/icon_contact.png" alt="Contact" align="center"/> Contact</a> </li> <li><span style="color:#ffffff;"> | </span></li> <li> <a href="loginAction" style="padding: 5px 5px"><img src="images/header/login_icon.png" alt="Login" align="center" /> Login</a> </li> </ul> </nav> </div> </td> </tr> </table> <table class="containerHeader"> <tr> <td> <div class="menu-wrap"> <nav class="menu" style="height: 0px;"> <ul class="clearfix" style="margin: 0px 0px 0px 12%;"> <li><a href="/homeAction">Home</a></li> <li ><a href="#">Search</a> <ul class="sub-menu"> <li> <a href="/../psrSearchAction.action">Protein, Sequence, or Reference Search</a> </li> <li> <a href="/../siteSearchAction.action">Site Search</a> </li> <li> <a href="/../siteSearchCompAction.action">Comparative Site Search</a> </li> </ul> </li> <li><a href="#">Browse</a> <ul class="sub-menu"> <li> <a href="/../browseDiseaseAction.action">Disease</a> </li> <li> <a href="/../browseCellLineAction.action">CellLine</a> </li> <li> <a href="/../browseTissueAction.action">Tissue</a> </li> </ul> </li> <li> <a href="/../staticMotifAnalysis.action">Tools</a> <ul class="sub-menu"> <li> <a href="/../staticMotifAnalysis.action">Motif Analysis</a> </li> <li> <a href="/../sequenceLogoAction.action">Sequence Logo</a> </li> <li> <a href="/../kinaseLibraryAction.action">Kinase Prediction</a> </li> </ul> </li> <li><a href="#">Downloads</a> <ul class="sub-menu"> <li> <a href="/../staticDownloads.action">Datasets from PSP</a> </li> <li> <a href="/../Supplemental_Files.action">Supplemental Files</a> </li> </ul> </li> <li><a href="../staticAboutPhosphosite">About  </a> <ul class="sub-menu"> <li> <a href="/../staticAboutPhosphosite.action">Overview</a> </li> <li> <a href="/../staticUsingPhosphosite.action">Using</a> </li> <li> <a href="/../staticPrivacyCookiePolicy.action">Privacy & Cookies</a> </li> <li> <a href="/../staticCurationProcess.action">Curation Process</a> </li> <li> <a href="/../staticSiteStatistics.action">Site Statistics</a> </li> <li> <a href="/../staticCiteUs.action">How to cite us</a> </li> <li> <a href="/../staticLicensing.action">Licensing</a> </li> </ul> </li> </ul> </nav> </div> </td> </tr> </table> </div> <div id="titleMainHeader" class="titleMainHeaderText containerHeader"><a href="/homeAction"> Home </a> > Using PhosphoSite<sup>&reg</sup></div> <span class="htmlErrors">  </span>  <script type="text/javascript"> function changeImg(item, source) { var page = "http://cst-psp01.cellsignal.com:8080/jsp/UsingPhosphosite.jsp"; if (!page.match(item + ".jsp")) { document.getElementById(item).src = source; } } function checkErrors() { return true; } </script> <link media="all" rel="canonical" href="https://www.phosphosite.org/staticUsingPhosphosite" /> <style type="text/css"> table.tableCurvedCorners td { border: 1px solid #999999; background-color: #FFFFFF; } </style> <div class="dataContainer"> <p> <table width="980px" border="0" cellspacing="0" cellpadding="3" style="margin-left:5px"> <tr> <td colspan="3" valign="top"> This section provides a description of how to use PhoshoSite v1.0, as well as an animated Site Map that provides a virtual tour of the site. Please note that we are constantly updating the web pages. In order to view the most recent versions of our pages it is important to frequently discard cache or temporary internet files. </td> </tr> <tr> <td colspan="2" valign="top"> The <strong>Homepage </strong> is the starting point for querying PhosphoSite. The user can chose from two sorts of queries. </td> <td rowspan="3"> <a href="/staticTrainingTutorial" style="display:block;"><img src="images/tutorial_link.gif;jsessionid=7E06A24942DA02442F51DCFE19A442BE" width="288" height="191" border="1" style="border:solid 1px #999;"/></a> <a href="/staticTrainingTutorial" style="font-size:11px;">View the PhosphoSitePlus<sup>®</sup> Training Tutorial.</a> </td> </tr> <tr> <td valign="top">°</td> <td valign="top"> <strong>Simple Search</strong> allows the user to query the database to view all known phosphorylation sites in a specific protein. After you submit the name of a protein in the search interface, the <em>Search Results </em> page lists all proteins in PhosphoSite that contain the input name. If the desired protein is listed, the user can select that protein and will be taken to its corresponding <em>Phosphoprotein Page </em> (see below). </td> </tr> <tr> <td valign="top">°</td> <td valign="top"> <strong>Advanced Search</strong> allows the user to simultaneously search from up to five categories of information, including protein name, the residue number of the phosphorylation site, SwissProt ID, author name, PubMed ID, CST Catalog#, domain, and sequence. For example, a user may look for a particular author's paper about a specific protein. Searching for a given domain will list all proteins that have a phosphorylation site within the domain of interest. Searching for a sequence, the user can find all occurrences of the sequence in proteins curated into PhosphoSite, either occurring in a phosphorylation site or elsewhere in the protein. </td> </tr> </table> <br> <table width="980px" border="0" cellspacing="0" cellpadding="3" style="margin-left:5px"> <tr> <td colspan="2" valign="top">A <strong>Phosphoprotein Page </strong> summarizes information about the parent protein and the phosphorylation sites contained within it.</td> </tr> <tr> <td valign="top">°</td> <td> The <strong>Overview</strong> section at the top of the page includes a brief description of the protein, protein type, the protein accession number used as the reference sequence by PhosphoSite, alternative names, and molecular weight. An interactive isoelectric calculator allows the user to retrieve the predicted isoelectric points of various phosphorylated isoforms of the parent protein. Links to other useful resources include the Alliance for Cellular Signaling (AfCS) (<a href="#Ref"><u>1</u></a>), ScanSite (<a href="#Ref"><u>2</u></a>), solved 3D structures from PDB, and the Protein Kinase Resource (PKR) (<a href="#Ref"><u>3</u></a>). </td> </tr> <tr> <td valign="top">°</td> <td> The <strong>Domains</strong> section in the middle of the page contains a linear schematic of the protein, predicted domains from Pfam (St. Louis) (<a href="#Ref"><u>4</u></a>), and the location of known phosphorylation sites relative to the domain structure. Phosphosites that have curated records are underlined and hyperlinked to the <em>Phosphorylation Site page</em> (see below). </td> </tr> <tr> <td valign="top">°</td> <td> The <strong>Sequences and Species</strong> section at the bottom page presents the phosphorylated residue and the surrounding amino acids (+/- 7) in the parent protein and orthologous sites in other species. Those residues that are underlined and hyperlinked are known to be phosphorlated <em>in vivo </em>: clicking on the link will take you to the Phosphorylation Site page (see below). A small icon next to the phosphosite indicates that phosphosite-specific antibodies or other probes are available for this site: clicking on the link will take you to either the CST catalog or to other sources of such reagents. </td> </tr> </table> <br> <table width="980px" border="0" cellspacing="0" cellpadding="3" style="margin-left:5px"> <tr> <td colspan="2" valign="top">The <strong>Phosphorylation Site page</strong> serves information specific to the selected phosphosite. It contains three sections.</td> </tr> <tr> <td valign="top">°</td> <td> The <strong>Phosphosite Information</strong> section at the top of the page includes the phosphorylated residue and its surrounding sequence (+/- 7 residues), a link to Scansite to predict likely sites for protein phosphorylation by particular kinases and likely sites for interaction with other signaling proteins (<a href="#Ref"><u>2</u></a>), orthologous sites in other species, and a Blast search of the site against NCBI, SwissProt and PDB. </td> </tr> <tr> <td valign="top">°</td> <td> The middle section contains information that has been curated about the phosphorylation site and may include information about how the site was experimentally characterized (mass spectrometry, phospho-antibodies, etc.), the apparent regulation of phosphorylation by kinases, phosphatases, ligands, and receptors, and downstream regulation. </td> </tr> <tr> <td valign="top">°</td> <td> The bibliography contains a hyperlinked references relating specifically to this phosphorylation site that have been curated into PhosphoSite.</td> </tr> </table> </br> <p><strong style="margin-left:10px">HTML5 Supported Browsers:</strong></p> <table border="0" cellpadding="0" cellspacing="0" class="tableCurvedCorners" style="margin-left:20px"> <thead> <tr><th>Browser Name</th><th>Version</th></tr> </thead> <tbody> <tr><td> Internet Explorer</td><td>9 and above</td></tr> <tr><td>Firefox</td><td>3.6 and above</td></tr> <tr><td>Google Chrome</td><td>30 and above</td></tr> <tr><td>Safari</td><td>4.0 and above</td></tr> <tr><td>Opera</td><td>12.10 and above</td></tr> <tr><td>Microsoft Edge</td><td>12 and above</td></tr> </tbody> </table> </br> <table width="980px" border="0" cellspacing="0" cellpadding="3" style="margin-left:5px"> <tr> <td valign="top"> <p> <strong>References:</strong><a name="Ref"></a> <ol> <li> Li J, Ning Y, Hedley W, Saunders B, Chen Y, Tindill N, Hannay T, Subramaniam S. (2002). The Molecule Pages database. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12478304&dopt=Abstract%20" target="_blank"><u>Nature. <em>420 </em>,716-7</u></a>. </li> <br> <li> Obenauer,J.C., Cantley,L.C., and Yaffe,M.B. (2003). Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12824383&dopt=Abstract" target="_blank"><u>Nucleic Acids Res. <em>31</em>,3635</u></a>. </li> <br> <li> Protein Kinase Resource (PKR) <a href="http://pkr.sdsc.edu/html/index.shtml" target="_blank"><u>http://pkr.sdsc.edu/html/index.shtml</u></a></li> <br> <li> Bateman A, Birney E, Cerruti L, Durbin R, Etwiller L, Eddy SR, Griffiths-Jones S, Howe KL, Marshall M, Sonnhammer EL. (2002) The Pfam protein families database. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11752314&dopt=Abstract" target="_blank"><u>Nucleic Acids Res. 30:276</u></a>. </li> </ol> </p> </td> </tr> </table> </p> </div> <style type="text/css"> .footer { display: none; position: absolute; left: 0; bottom: 0; height: auto; width: 100%; } .footerContent { width: 100%; background: #3e7998 url(//media.cellsignal.com/www/images/project-images/img/assets/background-header@15.png) no-repeat scroll bottom center; } .containerFooter { width: 1000px; position:relative; margin:auto; padding: 5px 0px 5px 20px; } .containerFooter td { color: white; } .containerFooter tr { vertical-align: middle; } .containerFooter img { vertical-align: middle; } .bottomHeader { width: 1000px; margin:auto; text-align: center; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; color: black; } </style>  <div class="footer"> <div class="footerContent"> <table width="100%" border="0" cellspacing="0" cellpadding="1" class="containerFooter"> <tr> <td width="25%" valign="top">Developed with grants from  <a href="https://www.nih.gov/" target="_blank"> <img src="images/footer/logo_nih.png" alt="NIH Logo" /> </a> </td> <td width="32%" valign="top">and literature mining with Linguamatics  <a href="https://www.linguamatics.com/" target="_blank"> <img src="images/footer/logo_i2e.png" alt="I2E Logo" /> </a> </td> <td> <a href="https://creativecommons.org/licenses/by-nc-sa/3.0/"> <img src="images/footer/logo_ccLicense.png" alt="Creative Commons License" align="left" height="30px"/> </a>PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. </td> </tr> </table> </div> <div style="background-color: #666666;"> <p class="bottomHeader" style="padding: 5px 0px;color:#FFFFFF">©2003-2024 Cell Signaling Technology, Inc. </p> </div> </div> <script>(function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'8ebdcb610b58896b',t:'MTczMzE2ODQ4Ny4wMDAwMDA='};var a=document.createElement('script');a.nonce='';a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();</script></body> </html>

CINXE.COM

PhosphoSite