Addgene: His-NavMs

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src="https://static.addgene.org/addgene-core/bd1910a15b/js/public/es5array-polyfill.js"></script> <script>(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-PWRGQMJ');</script> <script>/*<![CDATA[*/window.zEmbed||function(e,t){var n,o,d,i,s,a=[],r=document.createElement("iframe");window.zEmbed=function(){a.push(arguments)},window.zE=window.zE||window.zEmbed,r.src="javascript:false",r.title="",r.role="presentation",(r.frameElement||r).style.cssText="display: none",d=document.getElementsByTagName("script"),d=d[d.length-1],d.parentNode.insertBefore(r,d),i=r.contentWindow,s=i.document;try{o=s}catch(c){n=document.domain,r.src='javascript:var d=document.open();d.domain="'+n+'";void(0);',o=s}o.open()._l=function(){var o=this.createElement("script");n&&(this.domain=n),o.id="js-iframe-async",o.src=e,this.t=+new Date,this.zendeskHost=t,this.zEQueue=a,this.body.appendChild(o)},o.write('<body onload="document._l();">'),o.close()}("https://assets.zendesk.com/embeddable_framework/main.js","addgene.zendesk.com");/*]]>*/</script> <script> window.zESettings = { webWidget: { helpCenter: { title: { '*': 'Addgene Help Center' }, searchPlaceholder: { '*': 'Search our Help Center' }, messageButton: { '*': 'Contact Us' } }, contactForm: { title: { '*': 'Contact Us' } }, color: { theme: '#0056b3', launcher: '#0056b3' }, launcher: { label: { '*': 'Help Center' } } } }; </script> <title>Addgene: His-NavMs</title> <meta name="description" content="Plasmid His-NavMs from Dr. Paul DeCaen's lab contains the insert His-NavMs, Voltage gated sodium channel and is published in Nat Commun. 2017 Feb 16;8:14205. doi: 10.1038/ncomms14205. This plasmid is available through Addgene."> <meta name="keywords" content=""> </head> <body class="addgene-item-page"> <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-PWRGQMJ" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript> <div id="seq-popover-content" class="hidden"> <ul class="list-unstyled"> <li><p><b>Image: </b> Illustrated plasmid map in PNG format</p></li> <li><p><b>GenBank File: </b> Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence.</p></li> <li><p><b>SnapGene File: </b> Plasmid sequence and SnapGene enhanced annotations. Use with <a href="https://www.snapgene.com/try-snapgene/" target="_blank" rel="noopener noreferrer"><span class="glyphicon glyphicon-new-window" style="font-size: 12px;"></span> SnapGene software</a> or the <a href="https://www.snapgene.com/snapgene-viewer/" target="_blank" rel="noopener noreferrer"><span class="glyphicon glyphicon-new-window" style="font-size: 12px;"></span> free Viewer</a> to visualize additional data and align other sequences.</p></li> </ul> </div> <div class="modal plasmid-modal" id="modal-snapgene-192452" tabindex="-1" role="dialog" aria-labelledby="modal-snapgene-192452-label" aria-hidden="true"> <div class="modal-dialog modal-lg"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"> <span aria-hidden="true">×</span> <span class="sr-only">Close</span> </button> <h4 class="modal-title" id="modal-snapgene-192452-label"> Addgene Full Sequence Map for <span class="material-name">His-NavMs</span> </h4> </div> <div class="modal-body"> <img src="https://media.addgene.org/snapgene-media/v2.0.0/sequences/192452/aa9b7af1-cd00-4e74-973b-43fca569623e/addgene-plasmid-100004-sequence-192452-map.png" id="modal-snapgene-png-192452" alt="192452_map" style="width: 100%; max-width: 800px; "> </div> <div class="modal-footer"> <div class="items-flex-row-align-center"> <div style=""> Download File <a type="button" class="file-explainer sequence-policy-accept-toggle" data-placement="auto top" data-popover-content="#seq-popover-content" data-toggle="popover" data-trigger="focus" role="button" tabindex="0"> <span class="glyphicon glyphicon-info-sign icon-link"></span> </a> <ul class="download-nav-list"> <li> <a href="https://media.addgene.org/snapgene-media/v2.0.0/sequences/192452/aa9b7af1-cd00-4e74-973b-43fca569623e/addgene-plasmid-100004-sequence-192452-map.png" id="modal-snapgene-png-download-192452" title="PNG file download" download> Image</a> </li> <li> <a href="https://media.addgene.org/snapgene-media/v2.0.0/sequences/192452/aa9b7af1-cd00-4e74-973b-43fca569623e/addgene-plasmid-100004-sequence-192452.gbk" id="genbank-100004-seq-192452" class="genbank-file-download" data-id="192452" title="GenBank file download" download="100004-seq-192452.gbk"> GenBank</a> </li> <li> <a href="https://media.addgene.org/snapgene-media/v2.0.0/sequences/192452/aa9b7af1-cd00-4e74-973b-43fca569623e/addgene-plasmid-100004-sequence-192452.dna" id="snapgene-100004-seq-192452" class="snpagene-file-download" data-id="192452" title="SnapGene file download" download="100004-seq-192452.gbk"> SnapGene</a> </li> </ul> </div> <div> <a class="btn btn-primary" href="/100004/sequences/"> View all sequences </a> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div> </div> </div> <div class="modal fade in" id="campaign-modal" tabindex="-1" aria-hidden="true"> <div class="modal-dialog modal-dialog-centered"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"> <span aria-hidden="true">脳</span> </button> <h3 class="modal-title">Addgene Website Feedback</h3> </div> <div id="campaign-content" class="modal-body"> </div> <div class="modal-footer"> <div class="items-flex-row-align-center"> <span> Need help? 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<li><a href="/browse/">Browse</a></li> <li class="breadcrumb-pi"> <a href="/browse/pi/3546/">Paul DeCaen</a> </li> <li class="breadcrumb-article"> <a href="/browse/article/28190013/"> Sula et al </a> </li> <li><span class="material-name">His-NavMs</span></li> </ul> </div> <div class="row addgene-item-main-row"> <div class="item-main-col col-xs-9"> <section class="panel panel-default addgene-panel-catalog-item addgene-panel-plasmid-item"> <div class="panel-heading"> <div class="row row-vertically-centered"> <h1 class="has-no-flame"> <span class="material-name">His-NavMs</span> <br> <small> (Plasmid #<span id="addgene-item-id">100004</span>) </small> </h1> <div id="print-link-container" class="text-right"> <a class="print-link" href="javascript:window.print();"> <span class="glyphicon glyphicon-print"></span> Print </a> </div> </div> </div> <div class="panel-body"> <div class="material-properties"> <div id="top-section" class="row"> <div id="description-container" class="col-xs-6"> <ul id="plasmid-description-list" class="list-unstyled"> <li> <div class="field"> <div class="field-label">Purpose</div> <div class="field-content"> Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP </div> </div> </li> <li> <div class="field"> <div class="field-label">Depositing Lab</div> <div class="field-content"> <a href="/browse/pi/3546/"> Paul DeCaen</a> </div> </div> </li> <li> <div class="field"> <div class="field-label">Publication</div> <div class="field-content"> <a href="/browse/article/28190013/"> <cite> Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10.1038/ncomms14205. </cite> </a> <span> <small> ( <a href="#how-to-cite">How to cite <span class="glyphicon glyphicon-arrow-down"></span></a>) </small> </span> </div> </div> </li> <li> <div class="field"> <div class="field-label">Sequence Information</div> <ul class="list-unstyled"> <li class="field"> <div id="sequence_information"> <a href="/100004/sequences/"> Sequences (1) </a> </div> </li> </ul> </div> </li> </ul> </div> <div id="maps-container" class="col-xs-6"> <div id="plasmid-sequence-maps" class="text-center"> <div class="tab-content"> <div class="tab-pane active" id="tab-snapgene-192452"> <div data-toggle="modal" data-target="#modal-snapgene-192452" data-remote="false"> <img src="https://media.addgene.org/snapgene-media/v2.0.0/sequences/192452/aa9b7af1-cd00-4e74-973b-43fca569623e/addgene-plasmid-100004-sequence-192452-map.png" id="tab-snapgene-png-192452" alt="192452_map" style="max-width: 325px; max-height: 205px; "> </div> <ul class="addgene-nav-list text-center"> <li> <a href="javascript:{};" data-toggle="modal" data-target="#modal-snapgene-192452"> Enlarge </a> </li> <li><a class="" href="/100004/sequences/">View all sequences</a></li> </ul> </div> </div> </div> </div> </div> <div id="detail-sections" class="row"> <div class="col-xs-12"> <section> <h2>Ordering</h2> <div class="panel panel-default availability-notice-wrapper"> <div class="panel-body"> <small class="notice"> This material is available to academics and nonprofits only. </small> </div> </div> <table class="add-to-cart-table table-striped table-condensed" id="ordering-table"> <thead> <tr> <th>Item</th> <th>Catalog #</th> <th>Description</th> <th>Quantity</th> <th colspan="2">Price (USD)</th> </tr> </thead> <tbody> <tr id="row-100004"> <td style="width: 7em;">Plasmid</td> <td style="width: 7em;">100004</td> <td> <span id="format-details-100004"> Standard format: Plasmid sent in bacteria as agar stab </span> </td> <td style="width: 5.25em;" class="text-center"> <span data-add-to-cart-quantity="1" data-item-id="100004">1</span> </td> <td class="text-left price-cell" style="width: 5em;"> $<span id="marginal-price-100004" data-marginal-price data-item-id="100004" data-marginal-price-item-data="{"itemId": "100004", "bulkGroup": "b1", "unitPrices": [{"minQuantity": 1, "bulkUnitPrice": 85.0}, {"minQuantity": 6, "bulkUnitPrice": 85.0}, {"minQuantity": 21, "bulkUnitPrice": 85.0}], "rider": null}" >85</span> </td> <td id="add-to-cart-100004" style="width: 6em;" class="text-center">  <div class="addgene-addtocart js-addtocart atc-popover atc-mini-popover"> <a id="add-to-cart-link-100004" href="/users/login/?next=/100004/" class="btn btn-md btn-addgene-yellow btn-add-to-cart" > Add to Cart </a> </div> </td> </tr> </tbody> </table> <p class="text-right"> <small> </small> </p> </section> </div> <div class="col-xs-6"> <section> <h2>Backbone</h2> <ul class="list-unstyled"> <li class="field"> <div class="field-label">Vector backbone</div> pTracer-CMV2, IRES GFP </li> <li class="field"> <div class="field-label">Backbone manufacturer</div> Thermofisher </li> <li class="field"> <span class="field-label"> Backbone size w/o insert (bp)</span> 6200 </li> <li class="field"> <span class="field-label">Total vector size (bp)</span> 7100 </li> <li class="field"> <div class="field-label">Vector type</div> Mammalian Expression </li> </ul> </section> <section> <h2>Growth in Bacteria</h2> <ul class="list-unstyled"> <li class="field"> <div class="field-label">Bacterial Resistance(s)</div> Ampicillin, 100 渭g/mL </li> <li class="field" id="growth_temp_100004"> <div class="field-label">Growth Temperature</div> 37掳C </li> <li class="field"> <div class="field-label">Growth Strain(s)</div> DH5alpha </li> <li class="field"> <div class="field-label">Copy number</div> High Copy </li> </ul> </section> </div> <div class="col-xs-6"> <section> <h2> Gene/Insert </h2> <ul class="list-unstyled"> <li class="field"> <div class="field-label">Gene/Insert name</div> His-NavMs, Voltage gated sodium channel </li> <li class="field"> <div class="field-label">Species</div> Magnetococcus marinus </li> <li class="field"> <div class="field-label">Insert Size (bp)</div> 882 </li> <li class="field"> <div class="field-label">Mutation</div> C-terminus from a codon-optimized synthetic gene, starting at residue H237 </li> <li class="field"> <div class="field-label">GenBank ID</div> <span>CP000471.1</span> </li> <li class="field"> <span class="field-label">Promoter</span> CMV </li> <li class="field"> <span class="field-label"> Tag / Fusion Protein</span> <ul class="addgene-document-list"> <li>Hexa-His tag followed by a thrombin cleavage site (N terminal on insert)</li> </ul> </li> </ul> </section> <section> <h2> Cloning Information </h2> <ul class="list-unstyled"> <li class="field"> <span class="field-label">Cloning method</span> Restriction Enzyme </li> <li class="field"> <span class="field-label">5′ cloning site</span> EcoRI (unknown if destroyed) </li> <li class="field"> <span class="field-label">3′ cloning site</span> NotI (unknown if destroyed) </li> <li class="field"> <span class="field-label">5′ sequencing primer</span> Unknown </li> <a href="/tools/reference/sequencing-primers/">(Common Sequencing Primers)</a> </ul> </section> <section id="resource-information"> <h2>Resource Information</h2> <ul class="list-unstyled bottom-spacing-2"> <li class="field"> <div class="field-label">Article Citing this Plasmid</div> <ul class="addgene-document-list"> <li><a href="/100004/citations/">1 Reference</a></li> </ul> </li> </ul> </section> <section id="terms-and-licenses-section"> <h2>Terms and Licenses</h2> <ul class="list-unstyled"> <li class="field"> <div class="field-label">Academic/Nonprofit Terms</div> <ul class="addgene-document-list"> <li><a href="/agreement/1/">UBMTA</a></li> </ul> </li> <li class="field"> <div class="field-label">Industry Terms</div> <ul class="addgene-document-list"> <li>Not Available to Industry</li> </ul> </li> </ul> <div class=" flatblock flatblock-third-party-trademarks " > <div class="flatblock-content"> <h5>Trademarks:</h5> <ul class="addgene-document-list"> <li>Zeocin® is an InvivoGen trademark.</li> </ul> </div> </div> </section> </div> <div class="col-xs-12" id="notes-sections"> <section> <h2>Depositor Comments</h2> <div style="word-break: break-word;"> <p>Note: The GFP variant in this plasmid is missing the last five amino acids compared to the closest reference sequence (AAC53663.1). The depositor has confirmed that this does not impact plasmid function.</p> </div> </section> </div> </div> </div> </div> </section> <section id="how-to-cite" class="panel panel-default"> <div class="panel-heading items-flex-row-align-center"> <span>How to cite this plasmid</span> <small> ( <a href="#">Back to top<span class="glyphicon glyphicon-arrow-up"></span></a>) </small> </div> <div class="panel-body"> <p>These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. </p> <ul class="list-unstyled"> <li> <p>For your <strong>Materials & Methods</strong> section:</p> <div class="indent well well-sm"> <small> His-NavMs was a gift from Paul DeCaen (Addgene plasmid # 100004 ; http://n2t.net/addgene:100004 ; RRID:Addgene_100004) </small> </div> </li> <li> <p>For your <strong>References</strong> section:</p> <div class="indent well well-sm"> <small> <cite> <strong>The complete structure of an activated open sodium channel</strong>. Sula A, Booker J, Ng LC, Naylor CE, DeCaen PG, Wallace BA. <i>Nat Commun. 2017 Feb 16;8:14205. doi: 10.1038/ncomms14205.</i> 10.1038/ncomms14205 <a href="https://www.ncbi.nlm.nih.gov/pubmed/28205548" target="_blank" rel="noopener noreferrer"> PubMed 28205548 </a> </cite> </small> </div> </li> </ul> </div> </section> </div> <div class="col-xs-3 addgene-sidebar"> <div class="panel panel-default"> <div class="panel-heading">Related items:</div> <div class="panel-body"> <ul> <li> <a href="/browse/article/28190013/">From this article</a> </li> <li> <a href="/browse/pi/3546/">Paul DeCaen Lab Plasmids</a> </li> </ul> </div> </div> <div class="panel panel-default"> <div class="panel-heading">Commonly requested with:</div> <div class="panel-body"> <ul> <li> <a class="material-name" href="/100015/">NsvBa</a> </li> <li> <a class="material-name" href="/59169/">pCAG-ChrimsonR-tdT</a> </li> <li> <a class="material-name" href="/98166/">p-mCherry-C1-Phobos</a> </li> <li> <a class="material-name" 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