Human/Mouse ATG7 Antibody (MAB6608) by R&D Systems, Part of Bio-Techne

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data-time_template="0.35072088241577" data-time_post_epcc="1.1076219081879" data-time_epcc="-0.35072088241577" data-render_time="Friday 22nd of November 2024 10:35:21 AM" data-fallback="FALSE" data-time_to_process_data="0.16274189949036" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="validation-badges-header-wrapper"> <a class="biological-validation mr-3 d-inline-flex mt-1" href="/reagents/antibodies/antibody-validation" target="_blank" title="Biological Validation"></a> </div> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Human/Mouse ATG7 Antibody </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">MAB6608</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="reviews_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="reviews_quicklink"> <a href="#reviews" id="review_count_quicklink" class="reviews" data-tab="2"> <span class="review_stars review_stars_5"></span> <span class="font-weight-bold"></span> (3) </a> </div> </div> <div id="citation_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="citation_quicklink"> <a href="#citations" id="citation_count" data-tab="2" class="citations"> <span class="icon-icon-product-citations d-none d-lg-inline pr-1"></span> Citations (18)  </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <div class="px-0 my-1" id="datasheet-summary-wrapper" data-section="section-base--top-jumplink"> <article class="top-jumplink-wrapper"> <span class="icon-icon-pdf text-error-red"></span> <a href="#product-documents" class="product-documents-top-link" rel="nofollow">Product Datasheet / COA / SDS</a> </article> </div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> <div class="row border-top mb-2" id="images-orderdetail-wrapper"> <div class="col-xl-6 mt-2" id="images-wrapper" data-section="image_summary"> <div id="image-tree" data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-2 d-none d-md-block"> <ul class="list-inline m-0"> <li data-id="1" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunocytochemistry_15243.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunocytochemistry_15243.jpg" alt="ATG7 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC)." title="ATG7 in RAW 264.7 Mouse Cell Line." data-caption="ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a href="/p/secondary-antibodies/mouse-igg-northernlights-nl557-conjugated-antibody_nl007" target="_self">NL007</a>) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunocytochemistry_15243.jpg" data-id="0" data-application="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunocytochemistry_15243.jpg" class="lazy" /> </div> </li> <li data-id="2" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunohistochemistry_10631.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunohistochemistry_10631.jpg" alt="ATG7 antibody in Human Brain by Immunohistochemistry (IHC-P)." title="ATG7 in Human Brain." data-caption="ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a href="/p/staining-kits-reagents/antigen-retrieval-reagent-basic_cts013" target="_self">CTS013</a>). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a href="/p/staining-kits-reagents/anti-mouse-hrp-dab-cell-tissue-staining-kit_cts002" target="_self">CTS002</a>) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunohistochemistry_10631.jpg" data-id="1" data-application="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunohistochemistry_10631.jpg" class="lazy" /> </div> </li> <li data-id="3" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Flow_Cytometry_19593.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Flow_Cytometry_19593.jpg" alt="Detection of ATG7 antibody in HeLa Human Cell Line antibody by Flow Cytometry." title="Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry." data-caption="HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # <a href="/p/isotype-controls/mouse-igg-1-isotype-control_mab002" target="_self">MAB002</a>, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # <a href="/p/staining-kits-reagents/flow-cytometry-fixation-buffer-1x_fc004" target="_self">FC004</a>) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # <a href="/p/staining-kits-reagents/flow-cytometry-permeabilization-wash-buffer-i-1x_fc005" target="_self">FC005</a>). View our protocol for Staining Intracellular Molecules." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Flow_Cytometry_19593.jpg" data-id="2" data-application="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Flow_Cytometry_19593.jpg" class="lazy" /> </div> </li> <li data-id="4" class="none"> <i data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Simple_Western_16409.jpg" data-alt="Detection of Human ATG7 antibody by Simple WesternTM." title="Detection of Human ATG7 by Simple Western<SUP>TM</SUP>." data-caption="Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" class="none"></i> </li> <li data-id="5" class="none"> <i data-src="https://resources.bio-techne.com/images/products/mab6608_human-mouse-atg7-mab-clone-683906-41202412514862.jpg" data-alt="Detection of Human ATG7 by Western Blot" title="Detection of Human ATG7 by Western Blot" data-caption="ATG5 and ATG7 enhanced autophagy and inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 and ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 and P62. The values were normalized to beta-actin. c C28I2 cells were double stained with LC3 (red) and DAPI (blue) and visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 24 h. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). d Qualitative analysis of LC3 fluorescence intensity of chondrocytes. The values were normalized to the NC group. e Western blotting analysis of PERK, p-PERK and Nrf2 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. f Qualitative analysis of PERK, p-PERK and Nrf2 were normalized to beta-actin. (1:NC, 2:Ad-GFP, 3:Ad-ATG5, 4:RAPA, 5:Ad-ATG7, 6:Ad-ATG5 + RAPA, 7:Ad-ATG5 + Ad-ATG7). Rapamycin (25 μM) used as a positive control Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" class="none"></i> </li> <li class="list-inline-item"> <div class="image-slider-container"> <div class="lightbox product-image-lightbox"> <div class="lightbox border text-bt-dark-blue text-center search_result_thumbnail_image bg-light-gray rounded" style="padding: 20px 10px" title="Detection of Human ATG7 by Western Blot" data-caption="ATG5 and ATG7 enhanced autophagy and inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 and ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 and P62. The values were normalized to beta-actin. c C28I2 cells were double stained with LC3 (red) and DAPI (blue) and visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 24 h. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). d Qualitative analysis of LC3 fluorescence intensity of chondrocytes. The values were normalized to the NC group. e Western blotting analysis of PERK, p-PERK and Nrf2 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. f Qualitative analysis of PERK, p-PERK and Nrf2 were normalized to beta-actin. (1:NC, 2:Ad-GFP, 3:Ad-ATG5, 4:RAPA, 5:Ad-ATG7, 6:Ad-ATG5 + RAPA, 7:Ad-ATG5 + Ad-ATG7). Rapamycin (25 μM) used as a positive control Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges='<span class="biological-validation"></span>' data-mdb-img="https://resources.bio-techne.com/images/products/mab6608_human-mouse-atg7-mab-clone-683906-41202412514862.jpg" data-id="3" data-application="https://resources.bio-techne.com/images/products/mab6608_human-mouse-atg7-mab-clone-683906-41202412514862.jpg"> +2 <br>images </div> </div> </div> </li> </ul> </div> <div class="col-md-10 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="rnd"> <img src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Western_Blot_11614.jpg" alt="Detection of Human ATG7 antibody by Western Blot." title="Detection of Human ATG7 by Western Blot." data-caption="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/mouse-igg-horseradish-peroxidase-conjugated-antibody_haf007" target="_self">HAF007</a>). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges='' data-id="0" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="MAB6608"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="MAB6608" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">MAB6608</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="MAB6608-SP" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">MAB6608-SP</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB6608" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> <div class="atc-container order_detail_wrapper conjugates-and-formulations-wrapper my-2 position-relative"> <div class="button-group text-right pl-2"> <a href="#conjugates-MAB6608" class="btn btn-secondary atc-view-pricing atc-view-pricing-products collapsed text-nowrap mb-2" data-id="conjugates-MAB6608" data-toggle="collapse" aria-expanded="false" aria-controls="conjugates">View Available Conjugates </a> </div> <div id="conjugates-MAB6608" class="conjugates-and-formulations-container collapse position-absolute w-100"> <div class="atc-container border rounded bg-white shadow p-2 mt-1"> <div class="commerce-atc-datasheet atc-block-collapsible" data-type="conjugates_and_formulations" data-blockid="mab6608-conjugate-atc-block"> <div id="mab6608-conjugate-atc-block" class="atc-lines"> <div class="atc-header row pb-1 m-0"> <div class="atc-head col-4 p-0 font-weight-bold d-sm-none d-md-block">Conjugate</div> <div class="atc-head col-4 p-0 font-weight-bold">Catalog #</div> <div class="atc-head col-4"></div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608U" data-titleoverwrite="Alexa Fluor 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-350-conjugated-antibody-683906_fab6608u">Alexa Fluor 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-350-conjugated-antibody-683906_fab6608u">FAB6608U</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-350-conjugated-antibody-683906_fab6608u" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608V" data-titleoverwrite="Alexa Fluor 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-405-conjugated-antibody-683906_fab6608v">Alexa Fluor 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-405-conjugated-antibody-683906_fab6608v">FAB6608V</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-405-conjugated-antibody-683906_fab6608v" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608G" data-titleoverwrite="Alexa Fluor 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-488-conjugated-antibody-683906_fab6608g">Alexa Fluor 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-488-conjugated-antibody-683906_fab6608g">FAB6608G</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-488-conjugated-antibody-683906_fab6608g" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608X" data-titleoverwrite="Alexa Fluor 532"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608x">Alexa Fluor 532</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608x">FAB6608X</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608x" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608T" data-titleoverwrite="Alexa Fluor 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-594-conjugated-antibody-683906_fab6608t">Alexa Fluor 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-594-conjugated-antibody-683906_fab6608t">FAB6608T</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-594-conjugated-antibody-683906_fab6608t" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608R" data-titleoverwrite="Alexa Fluor 647"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-647-conjugated-antibody-683906_fab6608r">Alexa Fluor 647</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-647-conjugated-antibody-683906_fab6608r">FAB6608R</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-647-conjugated-antibody-683906_fab6608r" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="IC6608R" data-titleoverwrite="Alexa Fluor 647"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-647-conjugated-antibody-683906_ic6608r">Alexa Fluor 647</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-647-conjugated-antibody-683906_ic6608r">IC6608R</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-647-conjugated-antibody-683906_ic6608r" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608N" data-titleoverwrite="Alexa Fluor 700"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-700-conjugated-antibody-683906_fab6608n">Alexa Fluor 700</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-700-conjugated-antibody-683906_fab6608n">FAB6608N</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-700-conjugated-antibody-683906_fab6608n" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608S" data-titleoverwrite="Alexa Fluor 750"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-750-conjugated-antibody-683906_fab6608s">Alexa Fluor 750</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/human-mouse-atg7-alexa-fluor-750-conjugated-antibody-683906_fab6608s">FAB6608S</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/human-mouse-atg7-alexa-fluor-750-conjugated-antibody-683906_fab6608s" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608A" data-titleoverwrite="Allophycocyanin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608a">Allophycocyanin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608a">FAB6608A</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608a" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608B" data-titleoverwrite="Biotin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608b">Biotin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608b">FAB6608B</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608b" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608CL1" data-titleoverwrite="CoraFluor 1"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608cl1">CoraFluor 1</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608cl1">FAB6608CL1</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608cl1" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608D" data-titleoverwrite="DyLight 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608d">DyLight 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608d">FAB6608D</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608d" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608E" data-titleoverwrite="DyLight 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608e">DyLight 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608e">FAB6608E</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608e" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608K" data-titleoverwrite="DyLight 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608k">DyLight 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608k">FAB6608K</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608k" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608L" data-titleoverwrite="DyLight 550"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608l">DyLight 550</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608l">FAB6608L</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608l" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608M" data-titleoverwrite="DyLight 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608m">DyLight 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608m">FAB6608M</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608m" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608W" data-titleoverwrite="DyLight 650"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608w">DyLight 650</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608w">FAB6608W</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608w" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608Y" data-titleoverwrite="DyLight 680"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608y">DyLight 680</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608y">FAB6608Y</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608y" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608Z" data-titleoverwrite="DyLight 755"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608z">DyLight 755</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608z">FAB6608Z</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608z" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608F" data-titleoverwrite="FITC"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608f">FITC</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608f">FAB6608F</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608f" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608H" data-titleoverwrite="HRP"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608h">HRP</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608h">FAB6608H</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608h" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608JF525" data-titleoverwrite="Janelia Fluor 525"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/atg7-antibody-683906_fab6608jf525">Janelia Fluor 525</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/atg7-antibody-683906_fab6608jf525">FAB6608JF525</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/atg7-antibody-683906_fab6608jf525" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB6608I" data-titleoverwrite="Janelia Fluor 549"> <div class="row my-2 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href="#product-documents"> Product Documents</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#specific-notices"> Specific Notices</a> </li> </ul> </div> <div id="key_details" class="py-5 bt-pp-border-bottom" data-section="section-base--key-details"> <h3>Key Product Details</h3> <div class="mt-5 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Validated by</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Biological Validation </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Species Reactivity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> Human, Mouse </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Human, Mouse, Rat </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> CyTOF-ready, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Flow Cytometry, Immunohistochemistry, Western Blot </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Unconjugated </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal Mouse IgG<sub>1</sub> Clone # 683906 </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/atg7/antibodies">View all ATG7 Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="MAB6608" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <EM>E. coli</EM>-derived recombinant human ATG7<br />Asn387-Gln570<br />Accession # O95352 </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Specificity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Mouse </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG<sub>1</sub> </div> </div> </div> </div> <div id="scientific-data" class="bt-pp-border-bottom py-5" data-anchor-label="Scientific Data" data-section="section-base--scientific-data"> <div> <h3>Scientific Data Images for Human/Mouse ATG7 Antibody </h3> </div> <div class="product-data-wrapper mt-5" id="scientific-data-table" data-scientific-count="3"> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Western_Blot_11614.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Western_Blot_11614.jpg" alt="Detection of Human ATG7 antibody by Western Blot." title="Detection of Human ATG7 by Western Blot." data-caption="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/mouse-igg-horseradish-peroxidase-conjugated-antibody_haf007" target="_self">HAF007</a>). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Detection of Human ATG7 by Western Blot.</h4> Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/mouse-igg-horseradish-peroxidase-conjugated-antibody_haf007" target="_self">HAF007</a>). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunocytochemistry_15243.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunocytochemistry_15243.jpg" alt="ATG7 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC)." title="ATG7 in RAW 264.7 Mouse Cell Line." data-caption="ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a href="/p/secondary-antibodies/mouse-igg-northernlights-nl557-conjugated-antibody_nl007" target="_self">NL007</a>) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." data-badges="" data-application="Immunocytochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">ATG7 in RAW 264.7 Mouse Cell Line.</h4> ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a href="/p/secondary-antibodies/mouse-igg-northernlights-nl557-conjugated-antibody_nl007" target="_self">NL007</a>) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunohistochemistry_10631.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Immunohistochemistry_10631.jpg" alt="ATG7 antibody in Human Brain by Immunohistochemistry (IHC-P)." title="ATG7 in Human Brain." data-caption="ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a href="/p/staining-kits-reagents/antigen-retrieval-reagent-basic_cts013" target="_self">CTS013</a>). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a href="/p/staining-kits-reagents/anti-mouse-hrp-dab-cell-tissue-staining-kit_cts002" target="_self">CTS002</a>) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." data-badges="" data-application="Immunohistochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">ATG7 in Human Brain.</h4> ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a href="/p/staining-kits-reagents/antigen-retrieval-reagent-basic_cts013" target="_self">CTS013</a>). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a href="/p/staining-kits-reagents/anti-mouse-hrp-dab-cell-tissue-staining-kit_cts002" target="_self">CTS002</a>) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. </div> </div> </div> <span class="product-data-wrapper table" id="more-scientific-data" style="display:none"> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Flow_Cytometry_19593.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Flow_Cytometry_19593.jpg" alt="Detection of ATG7 antibody in HeLa Human Cell Line antibody by Flow Cytometry." title="Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry." data-caption="HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # <a href="/p/isotype-controls/mouse-igg-1-isotype-control_mab002" target="_self">MAB002</a>, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # <a href="/p/staining-kits-reagents/flow-cytometry-fixation-buffer-1x_fc004" target="_self">FC004</a>) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # <a href="/p/staining-kits-reagents/flow-cytometry-permeabilization-wash-buffer-i-1x_fc005" target="_self">FC005</a>). View our protocol for Staining Intracellular Molecules." data-badges="" data-application="Intracellular Staining by Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry.</h4> HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # <a href="/p/isotype-controls/mouse-igg-1-isotype-control_mab002" target="_self">MAB002</a>, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # <a href="/p/staining-kits-reagents/flow-cytometry-fixation-buffer-1x_fc004" target="_self">FC004</a>) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # <a href="/p/staining-kits-reagents/flow-cytometry-permeabilization-wash-buffer-i-1x_fc005" target="_self">FC005</a>). View our protocol for Staining Intracellular Molecules. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Simple_Western_16409.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/ATG7_MAB6608_Simple_Western_16409.jpg" alt="Detection of Human ATG7 antibody by Simple WesternTM." title="Detection of Human ATG7 by Simple Western<SUP>TM</SUP>." data-caption="Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Detection of Human ATG7 by Simple Western<sup>TM</sup>.</h4> Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/mab6608_human-mouse-atg7-mab-clone-683906-41202412514862.jpg" data-srcset="https://resources.bio-techne.com/images/products/mab6608_human-mouse-atg7-mab-clone-683906-41202412514862.jpg" alt="Detection of Human ATG7 by Western Blot" title="Detection of Human ATG7 by Western Blot" data-caption="ATG5 and ATG7 enhanced autophagy and inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 and ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 and P62. The values were normalized to beta-actin. c C28I2 cells were double stained with LC3 (red) and DAPI (blue) and visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 24 h. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). d Qualitative analysis of LC3 fluorescence intensity of chondrocytes. The values were normalized to the NC group. e Western blotting analysis of PERK, p-PERK and Nrf2 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. f Qualitative analysis of PERK, p-PERK and Nrf2 were normalized to beta-actin. (1:NC, 2:Ad-GFP, 3:Ad-ATG5, 4:RAPA, 5:Ad-ATG7, 6:Ad-ATG5 + RAPA, 7:Ad-ATG5 + Ad-ATG7). Rapamycin (25 μM) used as a positive control Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <div class="validation-badges-header-wrapper"> <a class="biological-validation mr-3 d-inline-flex mt-1" href="/reagents/antibodies/antibody-validation" target="_blank" title="Biological Validation"></a> </div> <h4 class="font-weight-bold">Detection of Human ATG7 by Western Blot</h4> ATG5 and ATG7 enhanced autophagy and inhibited ER stress in chondrocyte. a Western blotting analysis of LC3, P62, ATG5, ATG7 and ATG5-ATG12 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. b Qualitative analysis of ATG5, ATG7, ATG5-ATG12, LC3 and P62. The values were normalized to beta-actin. c C28I2 cells were double stained with LC3 (red) and DAPI (blue) and visualized by confocal microscopy (400X) after treated with Rapamycine, Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 24 h. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). d Qualitative analysis of LC3 fluorescence intensity of chondrocytes. The values were normalized to the NC group. e Western blotting analysis of PERK, p-PERK and Nrf2 expression after infected with Ad-ATG5, Ad-ATG7 and Ad-ATG5 + Ad-ATG7 in the C28I2 cells. beta-actin is served as an internal control. f Qualitative analysis of PERK, p-PERK and Nrf2 were normalized to beta-actin. (1:NC, 2:Ad-GFP, 3:Ad-ATG5, 4:RAPA, 5:Ad-ATG7, 6:Ad-ATG5 + RAPA, 7:Ad-ATG5 + Ad-ATG7). Rapamycin (25 μM) used as a positive control Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by R&D Systems. </div> </div> </span> <div class="row"> <div class="col-md-2"> <a id="scientific-view-more" class="more"> View 3 more images </a> </div> </div> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications for Human/Mouse ATG7 Antibody </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>CyTOF-ready</h4> </div> <div class="col-lg-9 border align-top py-2"> Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunocytochemistry</h4> </div> <div class="col-lg-9 border align-top py-2"> 8-25 µg/mL <br/>Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunohistochemistry</h4> </div> <div class="col-lg-9 border align-top py-2"> 8-25 µg/mL <br/>Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Intracellular Staining by Flow Cytometry</h4> </div> <div class="col-lg-9 border align-top py-2"> 0.25 µg/10<sup>6</sup> cells <br/>Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Simple Western</h4> </div> <div class="col-lg-9 border align-top py-2"> 20 µg/mL <br/>Sample: HeLa human cervical epithelial carcinoma cell line </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Western Blot</h4> </div> <div class="col-lg-9 border align-top py-2"> 2 µg/mL <br/>Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line </div> </div> </div> <div class="product-data-wrapper mt-5"> Please Note: Optimal dilutions of this antibody should be experimentally determined. </div> <h4 class="mt-3">Reviewed Applications</h4> <p><span class="review_stars review_stars_5"></span> Read <a href="#reviews" class="reviews">3 reviews</a> rated 5 using MAB6608 in the following applications:</p> <ul class="list-unstyled"> <li><a href="#reviews" class="reviews" data-application="Immunohistochemistry">Immunohistochemistry (2 Reviews) </a></li> <li><a href="#reviews" class="reviews" data-application="Western Blot">Western Blot (1 Review) </a></li> </ul> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Reconstitution</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="atc-container"> <div class="commerce-atc-reconstitution atc-block" data-type="reconstitution" data-blockid="ReconstitutionCart"> <div class="commerce-atc-reconstitution__instructions"> Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration. </div> <p class="atc-modal"></p> <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode=""> <div class="commerce-atc-reconstitution-header atc-header col-four row border-top pt-2 m-0"> <div class="commerce-atc-catnum atc-head col-md-6 font-weight-bold p-0">Reconstitution Buffer Available:</div> <div class="commerce-atc-price atc-head col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-md-3 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ReconstitutionCart" class="atc-lines"> <div class="commerce-atc-reconstitution-line productline col-four row py-2 m-0" data-catalog="RB01"> <div class="commerce-atc-title col-md-6 p-0"> <a href="">RB01: </a> </div> <div class="commerce-atc-price col-md-3"> </div> <div class="commerce-atc-quantity col-md-3 text-right p-0"> </div> </div> </div> <div class="atc-control ReconstitutionCart-control text-right mt-2"> <input type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc col-xs-4"/> </div> </div> </form> </div> </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability & Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL> </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: ATG7</h3> <article class="product-data-wrapper"> <P style="LINE-HEIGHT: normal; MARGIN: 0in 0in 0pt" class=MsoNormal>ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></P> </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> ATG7 Autophagy Related 7 Homolog </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> APG7, APG7L, GSA7 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Entrez Gene IDs</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.ncbi.nlm.nih.gov/gene?term=10533" class="a___link-external" target="_blank" rel="nofollow">10533</a> (Human); <a href="https://www.ncbi.nlm.nih.gov/gene?term=74244" class="a___link-external" target="_blank" rel="nofollow">74244</a> (Mouse); <a href="https://www.ncbi.nlm.nih.gov/gene?term=312647" class="a___link-external" target="_blank" rel="nofollow">312647</a> (Rat); <a href="https://www.ncbi.nlm.nih.gov/gene?term=856576" class="a___link-external" target="_blank" rel="nofollow">856576</a> (Yeast) </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Gene Symbol</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> ATG7 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>UniProt</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.uniprot.org/uniprot/?query=O95352" class="a___link-external" target="_blank" rel="nofollow">O95352</a> </div> </div> </div> <h4 class="mt-3">Additional ATG7 Products</h4> <ul class="list-unstyled"> <li><a href="/t/atg7" data-product="ATG7">All Products for ATG7 </a></li> <li><a href="/t/atg7/elisa-kits" data-application="ATG7 ELISA Kits">ATG7 ELISA Kits </a></li> <li><a href="/t/atg7/lysates" data-application="ATG7 Lysates">ATG7 Lysates </a></li> <li><a href="/t/atg7/antibodies" data-application="ATG7 Primary Antibodies">ATG7 Primary Antibodies </a></li> <li><a href="/t/atg7/proteins-enzymes" data-application="ATG7 Proteins and Enzymes">ATG7 Proteins and Enzymes </a></li> </ul> </div> <div id="product-documents" data-section="section-base--product-documents"> <div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3"> Product Documents for Human/Mouse ATG7 Antibody </h3> </div> <div class="vtab-section" data-section="Product Datasheets"> <div class="card-header rounded-top"> <a class="" data-toggle="collapse" data-target="#collapse_two" aria-expanded="true" aria-controls="collapse_two" id="product-datasheets-link"> <h5 class="mb-0"> Product Datasheets </h5> </a> </div> <div id="collapse_two" class="collapse show prod_datasheet_link" aria-labelledby="heading_two" data-parent="#accordion"> <div class="card-body"> <div class="row"> <div class="col-md-6"> <a href="https://resources.rndsystems.com/pdfs/datasheets/mab6608.pdf" class="datasheet_link" target="_blank" rel="nofollow">Download Product Datasheets</a> </div> </div> </div> </div> </div> <div class="card-header"> <a data-toggle="collapse" data-target="#collapse_one" aria-expanded="false" class="collapsed" aria-controls="collapse_one"> <h5 class="mb-0"> COA </h5> </a> </div> <div id="collapse_one" class="collapse " aria-labelledby="heading_one" data-parent="#accordion"> <div class="card-body"> <form novalidate="novalidate" data-drupal-selector="product-coa-form" action="#coa-form" method="post" id="product-coa-form" accept-charset="UTF-8"> <div class="row"> <div class="col-12"> <h2>Certificate of Analysis</h2> <p>To download a Certificate of Analysis, please enter a lot number in the search box below.</p> <p><strong>Note:</strong> Certificate of Analysis not available for kit components.</p> </div> </div> <section class="row"> <div class="col-md-6"> <form novalidate="novalidate" data-drupal-selector="product-coa-form"> <div class="form-row align-items-center"> <div class="col-md-10 my-1"> <input data-drupal-selector="form-vinvbpfztxxfmzbohiivrijvpv-vfcjfmqujdeitbje" type="hidden" name="form_build_id" value="form-vInVbPfZtXXfMZBoHIIvriJvPv_vFCJfMquJdeItBJE" /> <input data-drupal-selector="edit-product-coa-form" type="hidden" name="form_id" value="product_coa_form" /> <input data-drupal-selector="edit-product-code" type="hidden" name="product_code" value="MAB6608" /> <div class="js-form-item form-item pb-3 js-form-type-textfield form-item-lot-number js-form-item-lot-number form-no-label"> <input autocomplete="off" 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Calculators</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#flow-cytometry"> Flow Cytometry</a> </li> </ul> </div> <section id="reconstitution_calculator-tab-content-placeholder" class="tab-content-placeholder" data-id="reconstitution_calculator" data-product_code="mab6608"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> <section id="flow_cytometry-tab-content-placeholder" class="tab-content-placeholder" data-id="flow_cytometry" data-product_code="mab6608"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> <div class="tab-pane fade" id="related_products_resources" role="tabpanel" aria-labelledby="related_products_resources-tab"> <div data-section="section-base--technical-anchors"> <ul class="product-pages-anchors nav mt-2"> </ul> </div> </div> </div> </div> </div> <div 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Human/Mouse ATG7 Antibody (MAB6608) by R&D Systems, Part of Bio-Techne