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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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$j('#navList').prepend(activeNav); } </script></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsysimage_528a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.full.high.jpg/1689357389861.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.full.high.jpg/1689357389861.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.320.high.jpg/1689357389861.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.480.high.jpg/1689357389861.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.320.medium.jpg/1689357389861.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.620.high.jpg/1689357389861.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.320.low.jpg/1689357389861.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/image_528a/foregroundimg.img.480.medium.jpg/1689357389861.jpg" alt='Graphical illustration of QuantiGene Plex technology that uses Luminex xMAP beads and branched DNA-based signal amplification to quantitate gene expression ' title='Graphical illustration of QuantiGene Plex Technology '></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsysimage_528a img{ width:100%; max-width: 380px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsysimage_528a .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsysimage_528a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><div class="h2"> Accurate and precise RNA quantitation—measure up to 80 genes in a single well </div></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>QuantiGene Plex assays provide a fast and high-throughput solution for multiplexed gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a 96- or 384-well plate. The QuantiGene Plex assay is hybridization-based and incorporates branched DNA (bDNA) technology, which uses signal amplification rather than target amplification for direct measurement of RNA transcripts. The assay is extremely easy to use with a simple ELISA-like workflow, and does not require RNA purification.</p></div></div><div container-id="cq-colctrl-lt8" comp-id="B3"><div class="text parbase section"><p><a class="btn btn-featured" href="/sg/en/home/global/forms/life-science/request-quote-quantigene-assays.html">Request a quote</a>  <a class="btn btn-primary" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/quantigene-plex-assay-ordering.html">Ordering information</a></p></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_2d0"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.full.high.jpg/1689357389986.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.full.high.jpg/1689357389986.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.320.high.jpg/1689357389986.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.480.high.jpg/1689357389986.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.320.medium.jpg/1689357389986.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.620.high.jpg/1689357389986.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.320.low.jpg/1689357389986.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_2d0/foregroundimg.img.480.medium.jpg/1689357389986.jpg" alt='Product image of Invitrogen Immune Response 80-Plex Human QuantiGene Plex Panel' title='Invitrogen Immune Response 80-Plex Human QuantiGene Plex Panel'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_2d0 img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_2d0 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_2d0 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="heading section"><h4><span class="label label-info">NEW</span> Invitrogen Immune Response 80-Plex Human QuantiGene Plex Panel </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="text parbase section"><p>Analyze 80 cytokine, chemokine, and growth factor RNA targets simultaneously for efficient immune response profiling, biomarker discovery, and validation.</p><p><a class="cta-link" href="/order/catalog/product/QGP-180-10080">Learn more</a></p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_f07a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.full.high.jpg/1689357390067.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.full.high.jpg/1689357390067.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.320.high.jpg/1689357390067.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.480.high.jpg/1689357390067.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.320.medium.jpg/1689357390067.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.620.high.jpg/1689357390067.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.320.low.jpg/1689357390067.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_8842/uipar/image_f07a/foregroundimg.img.480.medium.jpg/1689357390067.jpg" alt='Product image of Invitrogen Immune Response 80-Plex Human QuantiGene Plex Panel' title='QuantiGene Panel Configurator Online Tool'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_f07a img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_f07a .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_8842uiparimage_f07a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="heading section"><h4><span class="label label-info">NEW</span> Use the Invitrogen QuantiGene Plex Gene Expression Panel </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="text parbase section"><p>Browse hundreds of preconfigured and published panels using name, keyword, category, or gene target search. You can also create custom panels.</p><p><a class="cta-link" href="/order/quantigene-plex/#/">Start your design</a></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-eb5a1">Overview</a></li><li><a data-toggle="tab" href="#container-eb5a2">QuantiGene Plex Workflow</a></li><li><a data-toggle="tab" href="#container-eb5a3">384-well assay</a></li><li><a data-toggle="tab" href="#container-eb5a4">Specification and FAQs</a></li><li><a data-toggle="tab" href="#container-eb5a5">Resources</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-eb5a1" class="tab-pane active"><div class="container-eb5a1 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Unique benefits of QuantiGene Plex Gene Expression Assay </h2></div><div class="text parbase section"><ul><li><b>Increase efficiency</b>—harmonized gene and protein multiplex solution—QuantiGene and <a href="/sg/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/procartaplex-immunoassays.html">ProcartaPlex</a> matched panels are complementary, highly correlative and run on the same Luminex platform, seamlessly integrated for unparalleled insights</li><li><b>Excellent correlation with gold standard technology</b>—between qPCR and QuantiGene</li><li><b>Works with difficult sample types</b>—works with degraded and cross-linked RNA in FFPE tissues and directly with blood; no RNA purification required</li><li><b>True multiplexing</b>—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity, helping reduce the number of samples needed</li><li><b>Standardized platform</b>—96-well plate format compatible with Luminex 200, MAGPIX, and FlexMap3D systems and 384-well with FlexMap3D system</li><li><b>Simple workflow</b>—ELISA-like workflow for target hybridization and signal amplification with no reverse transcription needed</li><li><b>Large inventory of validated genes</b>—select from over 22,000 genes to create pathway- and disease-themed panels</li><li><b>Customization</b>—if we don’t have your gene(s), we can create your custom panel within 3–4 weeks<br>  </li></ul></div><div class="heading section"><h2 id="work"> How QuantiGene Plex Assay works </h2></div><div class="text parbase section"><p>The QuantiGene Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. The assay can be performed in 96- or 384-well plates, and signal is detected using a Luminex instrument. </p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="brightcoveplayer section"><div data-video="b457f527deac"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); 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height: auto;" src="/bin/brightcove/image.jpeg?id=5752102628001&key=3663210762001"/></a><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Invitrogen QuantiGene Plex Assay-How it Works", "description": "Animation video to illustrate how QuantiGene Plex gene expression assay works to provide precise and easy quantitation of mRNA or DNA copy number variation of up to 80 genes.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/5667c51d-cf12-4d90-a8a5-3e5135c09c15/09a2a616-80e6-4a55-a2a6-f44f9a16a960/160x90/match/image.jpg"], "uploadDate": "2018-03-15T16:30:23.732Z", "duration": "PT1M38.731S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/5752102628001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5752102628001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5752102628001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="heading section"><h3 id="video"> Video: QuantiGene Plex–How It Works animation </h3></div><div class="text parbase section"><p>QuantiGene Plex Assays incorporate branched DNA technology and Luminex xMAP technology for accurate multiplex gene expression profiling. Branched DNA assays allow for the direct measurement of RNA transcripts by using signal amplification rather than target amplification.</p></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div><div id="container-eb5a2" class="tab-pane"><div class="container-eb5a2 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> QuantiGene Plex Gene Expression Assay workflow </h2></div><div class="text parbase section"><p>The QuantiGene Plex Gene Expression Assay is a hybridization-based assay using xMAP Luminex beads and performed in 96- or 384-well plates. The assay is based on direct quantification of the RNA targets for multiplexing of 3 to 80 RNA targets and branched DNA (bDNA) signal amplification technology. On the first day the sample is lysed to release the RNAs and incubated overnight with target specific probe sets and Luminex capture beads. On the second day the signal amplification tree is built via sequential hybridization of Pre-amplifier, Amplifier, and Label Probe. Each amplification unit provides a 400x signal amplification and there are six amplification units per target RNA copy resulting in a 2,400x signal amplification per copy RNA. The signal is detected by using the fluorescent reporter molecule, phycoerythrin, on a Luminex instrument for readout and analysis.</p></div><div class="heading section"><h3> QuantiGene Plex Assay Workflow </h3></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.high.jpg/1689357390808.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.480.high.jpg/1689357390808.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.full.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.480.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.medium.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.620.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.low.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.480.medium.jpg/1689357390808.jpg" alt='Sample preparation for QuantiGene Plex assays is lysing samples to release stabilized RNAs. No RNA amplification is needed' title='Sample preparation for QuantiGene Plex assay'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><b>Step 1.</b><b>Sample preparation:</b> Samples are lysed to release and stabilize RNAs. The RNA assay works with a variety of samples such as: cultured cells, virus, bacteria, plant and animal tissues, FFPE tissues, whole blood and PAXGene blood, or purified RNA.</p></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Step 2. Target hybridization:</b> Overnight hybridization in the 96- or 384-well plates with the target specific probe sets panel (Capture extenders–CEs, Label Extenders–LEs, and Blocking probes).</p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.high.jpg/1689357390883.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.480.high.jpg/1689357390883.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.full.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.480.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.medium.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.620.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.low.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.480.medium.jpg/1689357390883.jpg" alt='Target hybridization step using QuantiGene Plex assay involves incubating Luminex beads with capture probes with the target sample RNA and labeled extenders, blocking probes, and capture extenders' title='Target hybridization step in the QuantiGene Plex assay'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4 img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.high.jpg/1689357390944.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.480.high.jpg/1689357390944.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.full.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.480.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.medium.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.620.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.low.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.480.medium.jpg/1689357390944.jpg" alt='Signal amplification step in QuantiGene Plex assay involves adding the preamplifier, amplifier, and labeled probe to Luminex beads with capture probes containing target RNA' title='Signal amplification in QuantiGene Plex assay '></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><b>Step 3. Signal amplification:</b> Signal amplification is achieved using branched DNA (bDNA) technology. First, an individual Pre-Amplifier molecule hybridizes to each pair of Label Extenders. Then, multiple Amplifier molecules hybridize to each Pre-Amplifier. Finally, multiple biotinylated Label Probe oligonucleotides hybridize to each Amplifier.</p></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Step 4. Detection:</b> Addition of streptavidin phycoerythrin (SAPE) generates a signal that is proportional with the amount of target RNA present in the sample. The signal is read using a Luminex instrument.</p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_f366"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.full.high.jpg/1689357391032.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.full.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.320.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.480.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.320.medium.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.620.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.320.low.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.480.medium.jpg/1689357391032.jpg" alt='Luminex 200 Instrument' title='Luminex 200 Instrument'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_f366 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_f366 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a"><a class="image-enlarge-link" data-lightbox="image_c88a" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/_jcr_content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/backgroundimg.img.jpg/1689357391116.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.high.jpg/1689357391090.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.480.high.jpg/1689357391090.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.full.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.480.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.medium.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.620.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.low.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.480.medium.jpg/1689357391090.jpg" alt='Example data from Applied Biosystems Transcriptome Analysis Console Software' title='Example gene expression data'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><b>Step 5. Data Analysis:</b> Analyze data using the <a href="/sg/en/home/digital-science/thermo-fisher-connect/all-analysis-modules.html">Thermo Fisher Connect cloud-based QuantiGene data analysis</a> application from any computer, anywhere. For advanced analysis and visualization, data can be analyzed using the free desktop application software, <a href="/sg/en/home/life-science/microarray-analysis/">Applied Biosystems Transcriptome Analysis Console (TAC) software</a>. TAC provides visualization and interpretation using tools such as scatter and volcano plots, hierarchical clustering, and provides link outs to publicly available annotations.</p></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div><div id="container-eb5a3" class="tab-pane"><div class="container-eb5a3 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2 id="quantigenePHTA"> QuantiGene Plex high throughput assays </h2></div><div class="text parbase section"><p>The QuantiGene Plex 384-well assay format enables multiplex gene expression analysis in a high throughput format. The assay uses target-specific probes to capture the RNA of interest and branched DNA technology to amplify fluorescent signals which are read with the <a href="/sg/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/luminex-instruments.html">FLEXMAP 3D</a>, Luminex platform. Automation and batch processing options for this assay format increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects.</p><p>The <a href="/order/catalog/product/QP1016">QuantiGene Plex 384-well Assay Kit</a> (Cat. No. QP1016) contains all the necessary reagents, buffers, and plates to perform QuantiGene Plex assays in 384-well plates. Design your target-specific QuantiGene Plex panel with our <a href="/order/quantigene-plex/configuration">Panel Configuration Tool</a> or <a href="/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html">contact</a> your local Technical Sales Specialist for more information.<br>  </p></div><div class="heading section"><h2> QuantiGene Plex 384-well example data </h2></div><div class="text parbase section"><p>The QuantiGene Plex 384-well assay is designed to increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects. Below is example data that was generated using the 384-well format using the recommended equipment listed below.</p><p><b>Stimulation of cytokine production in HeLa cells</b><br> Cytokine production in cultured HeLa cells was stimulated using a Cell Stimulation Cocktail and monitored for various time points up to 24 hours. In this experiment, a full 80-plex panel was used for screening purposes focused on cytokine, and chemokine biomarkers.</p></div><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb"><a class="image-enlarge-link" data-lightbox="image_eccb" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/_jcr_content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/backgroundimg.img.jpg/1689357390546.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.low.jpg/1689357390524.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.medium.jpg/1689357390524.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.480.medium.jpg/1689357390524.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.620.high.jpg/1689357390524.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.full.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.480.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.medium.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.620.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.low.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.480.medium.jpg/1689357390524.jpg" alt='Example data from QuantiGene Plex 384-well assay showing cytokine production stimulation in HeLa cells' title='Example data from QuantiGene Plex 384-well assay'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb img{ width:100%; max-width: 1000px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="text parbase section"><p><small><span class="muted"><b>Figure 1. Normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.</b> Y-axis: Average normalized expression; x-axis: mRNA targets; z-axis: time points of stimulation. Only a subset of gene targets tested is being displayed. HeLa cells were cultured in a 384-well format and stimulated with a Cell Stimulation cocktail for various times up to 24h (1h, 2h, 4h, 24h). Unstimulated cells served as baseline control. At the indicated time points, cells were lysed according to the <a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017267_QuantiGene_Cultured_Cells_PI.pdf" target="_blank">QuantiGene sample prep protocol</a> for cell lysates using liquid handling (<a href="/order/catalog/product/650-INSTR">Thermo Fisher Platemate</a>). After all samples from all time points were prepared, the QuantiGene Plex 384-well assay was performed. Chart illustrates the normalized gene expression results.</span></small></p></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>The normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail reveals the degree of up- or downregulation. The average normalized expression (y-axis) represents the level of gene induction for the mRNA targets listed on the x-axis, while the time points of stimulation are shown on the z-axis. Of note, for example IL1B, IL8 and TGFB1 show pronounced relative gene expression at various time points of stimulation. While TGFB1 relative expression seems to gradually increase over time, peak expression for IL1B is seen at later time points around 24h. Table 1 shows normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.</p></div><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73"><a class="image-enlarge-link" data-lightbox="image_2d73" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/_jcr_content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/backgroundimg.img.jpg/1689357390617.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.low.jpg/1689357390587.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.medium.jpg/1689357390587.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.480.medium.jpg/1689357390587.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.620.high.jpg/1689357390587.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.full.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.480.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.medium.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.620.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.low.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.480.medium.jpg/1689357390587.jpg" alt='Normalized gene expression of various target genes after stimulation of cytokine production in HeLa cells after 1, 2, 4, and 24 hours' title='Normalized gene expression of target genes after stimulation of cytokine production '></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73 img{ width:100%; max-width: 900px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div><div class="text parbase section"><p><small><span class="muted"><b>Table 1 and 2. Normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail. </b></span></small></p></div><div class="heading section"><h3> See how scientists are using QuantiGene Plex 384-well in their research: </h3></div><div class="text parbase section"><ol><li>Danahay et. al. (2015) <a href="https://www.sciencedirect.com/science/article/pii/S2211124714010468" target="_blank">Notch2 Is Required for Inflammatory Cytokine-Driven Goblet Cell Metaplasia in the Lung.</a><span class="icon icon-outbound"></span> Cell Reports 10:239-252.</li><li>Ferrer et al. (2014) <a href="https://pubmed.ncbi.nlm.nih.gov/24873859/" target="_blank">A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.</a><span class="icon icon-outbound"></span> Stem Cells Transl Med 2:911-922.</li><li>Severyn et al. (2016) <a href="https://pubmed.ncbi.nlm.nih.gov/27461835/" target="_blank">Development of a High-Throughput Gene Expression Screen for Modulators of RAS-MAPK Signaling in a Mutant RAS Cellular Context.</a><span class="icon icon-outbound"></span> J Biomol screen 9:989-997.</li><li>Neilsen et al. (2020) <a href="https://www.nature.com/articles/s41597-020-0365-2" target="_blank">High-throughput identification of protein functional similarities using a gene-expression-based siRNA screen</a>. Sci Data 7:27.<br>  </li></ol></div><div class="heading section"><h2> QuantiGene Plex instrumentation and equipment for high throughput and batch processing </h2></div><div class="text parbase section"><p>Find instrumentation and equipment to make every step of your QuantiGene Plex 384-well workflow easy. For detailed options and recommendations for batch processing, refer to the <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019482_QuantigenePlexGeneExpressionAssay_384wells_UG.pdf" target="_blank">QuantiGene Plex 384-well manual</a> or <a href="/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html">contact</a> your local Technical Sales Specialist.</p><div class="table-component-wrapper"><table class="rte-table" id="table-851501985"><tbody><tr><th>Workflow step</th><th colspan="2">Recommended equipment</th><th>Description</th></tr><tr><td style="width: 20%;"><b>Sample incubation and target hybridization</b></td><td style="border-right: none;">Temperature regulated orbital shaker: <a href="/order/catalog/product/SHKE4450">MaxQ 4450 Benchtop Orbital Shakers</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/maxq-shaker.jpg" alt="MaxQ 4450 Benchtop Orbital Shakers" height="95"></td><td style="width: 40%;">Versatile shaker ideal for incubating a small number of vessels and cell culture and many other applications.</td></tr><tr><td><b>Plate washes</b></td><td colspan="2">Magnetic microplate washers</td><td>Automated magnetic microplate washer for 384-well plates.</td></tr><tr><td><b>Sample incubation</b></td><td style="border-right: none;">Plate sealer: <a href="/order/catalog/product/AB-1443A">ALPS 50 V-Manual Heat Sealer</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/alps-heat-sealer.jpg" alt="Thermo Scientific ALPS 50 V- Manual Heat Sealer" height="95"></td><td>Applies consistent, secure, tight seals around individual wells, eliminating sample loss through evaporation and cross contamination between wells.</td></tr><tr><td><b>Sample and plate additions</b></td><td style="border-right: none;"><a href="/order/catalog/product/650-INSTR">Versette Automated Liquid Handler</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/versette-handler.jpg" alt="Thermo Scientific Versette Automated Liquid Handler platform" height="95"></td><td>Compact liquid handler featuring user-friendly programming, reliable performance, and a choice of 96- or 384-channel pipetting heads.</td></tr><tr><td><b>Multiplex detection</b></td><td style="border-right: none;"><a href="/order/catalog/product/APX1342">Luminex FLEXMAP 3D Instrument System</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/luminex-instrument.jpg" width="95" alt="Luminex FLEXMAP 3D Instrument System"></td><td>Most advanced and multifunctional multiplex unit for simultaneous measurement of up to 80 tests in a single reaction volume.</td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-eb5a4" class="tab-pane"><div class="container-eb5a4 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> QuantiGene Plex specifications </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1803649485"><tbody><tr><td style="border: none;"> </td><th style="width: 28%;">Parameter</th><th style="width: 28%;">96-well</th><th style="width: 28%;">384-well</th></tr><tr><td style="background-color: #f3f3f3;" rowspan="6"><b>Product specification</b></td><td><b>Sensitivity (Limit of detection)</b></td><td>1,000–2,000 transcripts/assay well</td><td>2,000–4,000 transcripts/assay well</td></tr><tr><td><b>Assay variation (C.V.)</b></td><td>≤15%</td><td>≤20%</td></tr><tr><td><b>Linearity</b></td><td colspan="2">≥3 logarithmic units</td></tr><tr><td><b>Plex level</b></td><td>3–80</td><td>3–80</td></tr><tr><td><b>Samples</b></td><td colspan="2">Cultured cells, bacteria, whole blood, PAXgene blood or dried blood spots, fresh/frozen tissues (animal or plant), FFPE samples, purified RNA</td></tr><tr><td><b>Compatible Luminex instrumentation</b></td><td>MAGPIX, Luminex 200, FLEXMAP 3D, INTELLIFLEX</td><td>FLEXMAP 3D, INTELLIFLEX</td></tr><tr><td style="background-color: #f3f3f3;" rowspan="5"><b>Assay volumes</b></td><td><b>Target probe hybridization</b></td><td>100 µL</td><td>70 µL</td></tr><tr><td><b>Pre-amp hybridization</b></td><td>100 µL</td><td>30 µL</td></tr><tr><td><b>Amp hybridization</b></td><td>100 µL</td><td>30 µL</td></tr><tr><td><b>Label probe hybridization</b></td><td>100 µL</td><td>30 µL</td></tr><tr><td><b>SAPE</b></td><td>100 µL</td><td>30 µL</td></tr></tbody></table></div></div><div class="heading section"><h2> QuantiGene Plex FAQs </h2></div><div class="text parbase section"><ol><li><b>What sample types are compatible with QuantiGene Plex assay? <br></b>Whole blood, fresh tissue, cultured cells, and purified RNA are validated. Other sample types including Tempus blood, bacteria, H&E stained/unstained FFPE, and blood spots should also be applicable.</li><li><b>What data analysis software solutions are offered for the QuantiGene Plex assay?<br></b>Analyze data using the <a href="https://apps.thermofisher.com/apps/quantigene/">QuantiGene cloud-based application</a> from any computer, anywhere. For advanced analysis and visualize, data can be analyzed using the free desktop application software, <a href="/sg/en/home/life-science/microarray-analysis/">Applied Biosystems Transcriptome Analysis Console (TAC) software</a>.</li><li><b>Can the VorTemp™ 56 Shaking Incubator be used for hybridization?<br></b>We highly recommend the MaxQ Shaking incubator for optimal assay performance. It is possible to use the Vortemp, but 800 rpm is recommended.</li><li><b>What is the recommended temperature for hybridization?<br></b>The Day 1 temperature is 54°C while the Day 2 temperature is 50°C. Please use Quantigene Incubator Temperature Validation Kit (QS0517) to verify the incubator temperature.</li><li><b>What are the proper storage conditions for the QuantiGene Plex reagents?<br></b>When the kit arrives, please follow the instructions to store individual components. The Preamp/Amp/LP Diluent, Bead Mix, SAPE, SAPE Diluent should be stored at 4°C, while probe set mix, Proteinase K, and blocking reagent should be stored at -20°C. Others are stored at room temperature. Refer to the product insert for details.</li><li><b>What reagents are included in the kit?<br></b>The kit provides all necessary reagents to perform the assay except Target Probe and Bead mix which are ordered separately. See the user manual for a list of the reagents.</li><li><b>Can QuantiGene Plex 384-well assays be used for miRNA or DNA analysis?<br></b>No. We have options with the QuantiGene Singleplex assay for miRNA and DNA.</li><li><b>Should any control/housekeeping genes be used in my panel<br></b>Three or more control/housekeeping genes are recommended to normalize the gene expression results.</li><li><b>Can I use a heat sealer to seal the plate on day 2 of the assay<br></b>No, manual sealing using the provided seal is recommended. Heat sealing would deform the plate causing problem in washing.</li><li><b>How do I dilute my samples?<br></b>For cell lysate or blood lysate, please use Diluted Lysis Mix (DLM); for tissue homogenates, please use Homogenizing Solution.</li><li><b>How should I seal the Day 1 plate for Target Probe hybridization?<br></b>The Day 1 plate can be sealed either manually or using a heat sealer. The pressure seal for manual sealing and the heat seal AB-0757 for Thermo Fisher ALPS 50 V (AB-1443A) are provided in the kit. If a different heat sealer will be used, please consult the manufacturer for suitable sealing.</li></ol></div></div><div class="end"></div></div></div><div id="container-eb5a5" class="tab-pane"><div class="container-eb5a5 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Technical guides </h3></div><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/factors-affecting-blood-gene-expression-app-note.pdf" target="_blank">Factors affecting blood gene expression</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/LSG/brochures/defining-quantigene-plex-assay-panel-app-note.pdf" target="_blank">Defining a QuantiGene Plex panel</a></li></ul></div><div class="heading section"><h3> User manuals </h3></div><div class="text parbase section"><p>User manuals for use with assay kits QP1013, QP1014, QP1015 and QP1016</p><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/MAN0017862_quantigene-plex-gene-expression-assay-user-guide.pdf" target="_blank">QuantiGene Plex manual 3-64 plex and 65-80 plex assays</a></li><li><span class="icon icon-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019482_QuantigenePlexGeneExpressionAssay_384wells_UG.pdf" target="_blank">QuantiGene Plex 384-well assays</a></li></ul><p>QuantiGene sample processing</p><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf" target="_blank">Preparation of bacterial homogenates for use in QuantiGene Assays</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017267_QuantiGene_Cultured_Cells_PI.pdf" target="_blank">Cultured cells</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017268_QuantiGene_Fresh_Frozen_Tissues_PI.pdf" target="_blank">Fresh/frozen animal or plant tissues</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017265_QuantiGene_Blood_PI.pdf" target="_blank">Blood samples</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017266_QuantiGene_FFPE%20Tissues_PI.pdf" target="_blank">FFPE tissue samples</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/LSG/manuals/QG-Incubator-Temp-Validation-Kit_32016.pdf" target="_blank">QuantiGene incubator temperature validation kit</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/LSG/brochures/Data_Sheet_MS_3_digital.pdf" target="_blank">IKA plate shaker</a></li></ul></div><div class="heading section"><h3> Application notes </h3></div><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/quantigene-assays-app-note.pdf" target="_blank">QuantiGene Application Guide</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/determination-gene-signatures-subgroup-melanoma-dna-hybridization-assays-app-note.pdf" target="_blank">New determination of gene signatures to subgroup melanoma patients using novel branched DNA hybridization assays</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/transcript-regulation-prototypical-inducers-human-hepatocytes-app-note.pdf" target="_blank">Transcript regulation of 18 ADME genes by prototypical inducers in human hepatocytes using QuantiGene Plex Assays</a></li></ul></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> Overview </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-eb5a1"><div class="container-eb5a1 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Unique benefits of QuantiGene Plex Gene Expression Assay </h2></div><div class="text parbase section"><ul><li><b>Increase efficiency</b>—harmonized gene and protein multiplex solution—QuantiGene and <a href="/sg/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/procartaplex-immunoassays.html">ProcartaPlex</a> matched panels are complementary, highly correlative and run on the same Luminex platform, seamlessly integrated for unparalleled insights</li><li><b>Excellent correlation with gold standard technology</b>—between qPCR and QuantiGene</li><li><b>Works with difficult sample types</b>—works with degraded and cross-linked RNA in FFPE tissues and directly with blood; no RNA purification required</li><li><b>True multiplexing</b>—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity, helping reduce the number of samples needed</li><li><b>Standardized platform</b>—96-well plate format compatible with Luminex 200, MAGPIX, and FlexMap3D systems and 384-well with FlexMap3D system</li><li><b>Simple workflow</b>—ELISA-like workflow for target hybridization and signal amplification with no reverse transcription needed</li><li><b>Large inventory of validated genes</b>—select from over 22,000 genes to create pathway- and disease-themed panels</li><li><b>Customization</b>—if we don’t have your gene(s), we can create your custom panel within 3–4 weeks<br>  </li></ul></div><div class="heading section"><h2 id="work"> How QuantiGene Plex Assay works </h2></div><div class="text parbase section"><p>The QuantiGene Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. The assay can be performed in 96- or 384-well plates, and signal is detected using a Luminex instrument. </p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="brightcoveplayer section"><div data-video="6bc1e65d06fa"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-6bc1e65d06fa', 10); </script><div id="6bc1e65d06fa-Modal" class="modal modal-video" tabindex="-1" role="dialog" aria-labelledby="6bc1e65d06fa-Modal-Label" aria-hidden="true" style="display: none;"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">x</button></div><div data-video-modal-body="6bc1e65d06fa" class="modal-body"></div></div><script> $(document).ready(function(){ $('#6bc1e65d06fa').bind('click', function () { $.ajax({url: '/content/lifetech/ipac/en-sg/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a1/tab/brightcoveplayer_fe8.ajax.html?cached=false', data: { account: '3663210762001', videoPlayer: '5752102628001', playerPath: '/etc/brightcovetools/cbd-video-players/cbd-gwi-test-player' }, success: function(result) { $('[data-video-modal-body*=6bc1e65d06fa]').html(result); 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height: auto;" src="/bin/brightcove/image.jpeg?id=5752102628001&key=3663210762001"/></a><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Invitrogen QuantiGene Plex Assay-How it Works", "description": "Animation video to illustrate how QuantiGene Plex gene expression assay works to provide precise and easy quantitation of mRNA or DNA copy number variation of up to 80 genes.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/5667c51d-cf12-4d90-a8a5-3e5135c09c15/09a2a616-80e6-4a55-a2a6-f44f9a16a960/160x90/match/image.jpg"], "uploadDate": "2018-03-15T16:30:23.732Z", "duration": "PT1M38.731S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/5752102628001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5752102628001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5752102628001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="heading section"><h3 id="video"> Video: QuantiGene Plex–How It Works animation </h3></div><div class="text parbase section"><p>QuantiGene Plex Assays incorporate branched DNA technology and Luminex xMAP technology for accurate multiplex gene expression profiling. Branched DNA assays allow for the direct measurement of RNA transcripts by using signal amplification rather than target amplification.</p></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> QuantiGene Plex Workflow </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-eb5a2"><div class="container-eb5a2 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> QuantiGene Plex Gene Expression Assay workflow </h2></div><div class="text parbase section"><p>The QuantiGene Plex Gene Expression Assay is a hybridization-based assay using xMAP Luminex beads and performed in 96- or 384-well plates. The assay is based on direct quantification of the RNA targets for multiplexing of 3 to 80 RNA targets and branched DNA (bDNA) signal amplification technology. On the first day the sample is lysed to release the RNAs and incubated overnight with target specific probe sets and Luminex capture beads. On the second day the signal amplification tree is built via sequential hybridization of Pre-amplifier, Amplifier, and Label Probe. Each amplification unit provides a 400x signal amplification and there are six amplification units per target RNA copy resulting in a 2,400x signal amplification per copy RNA. The signal is detected by using the fluorescent reporter molecule, phycoerythrin, on a Luminex instrument for readout and analysis.</p></div><div class="heading section"><h3> QuantiGene Plex Assay Workflow </h3></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.high.jpg/1689357390808.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.480.high.jpg/1689357390808.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.full.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.480.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.medium.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.620.high.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.320.low.jpg/1689357390808.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_5afb/foregroundimg.img.480.medium.jpg/1689357390808.jpg" alt='Sample preparation for QuantiGene Plex assays is lysing samples to release stabilized RNAs. No RNA amplification is needed' title='Sample preparation for QuantiGene Plex assay'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_5afb .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><b>Step 1.</b><b>Sample preparation:</b> Samples are lysed to release and stabilize RNAs. The RNA assay works with a variety of samples such as: cultured cells, virus, bacteria, plant and animal tissues, FFPE tissues, whole blood and PAXGene blood, or purified RNA.</p></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Step 2. Target hybridization:</b> Overnight hybridization in the 96- or 384-well plates with the target specific probe sets panel (Capture extenders–CEs, Label Extenders–LEs, and Blocking probes).</p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.high.jpg/1689357390883.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.480.high.jpg/1689357390883.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.full.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.480.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.medium.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.620.high.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.320.low.jpg/1689357390883.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_83e4/foregroundimg.img.480.medium.jpg/1689357390883.jpg" alt='Target hybridization step using QuantiGene Plex assay involves incubating Luminex beads with capture probes with the target sample RNA and labeled extenders, blocking probes, and capture extenders' title='Target hybridization step in the QuantiGene Plex assay'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4 img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_83e4 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.high.jpg/1689357390944.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.480.high.jpg/1689357390944.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.full.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.480.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.medium.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.620.high.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.320.low.jpg/1689357390944.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_4a8f/foregroundimg.img.480.medium.jpg/1689357390944.jpg" alt='Signal amplification step in QuantiGene Plex assay involves adding the preamplifier, amplifier, and labeled probe to Luminex beads with capture probes containing target RNA' title='Signal amplification in QuantiGene Plex assay '></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_4a8f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><b>Step 3. Signal amplification:</b> Signal amplification is achieved using branched DNA (bDNA) technology. First, an individual Pre-Amplifier molecule hybridizes to each pair of Label Extenders. Then, multiple Amplifier molecules hybridize to each Pre-Amplifier. Finally, multiple biotinylated Label Probe oligonucleotides hybridize to each Amplifier.</p></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><b>Step 4. Detection:</b> Addition of streptavidin phycoerythrin (SAPE) generates a signal that is proportional with the amount of target RNA present in the sample. The signal is read using a Luminex instrument.</p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_f366"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.full.high.jpg/1689357391032.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.full.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.320.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.480.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.320.medium.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.620.high.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.320.low.jpg/1689357391032.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_f366/foregroundimg.img.480.medium.jpg/1689357391032.jpg" alt='Luminex 200 Instrument' title='Luminex 200 Instrument'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_f366 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_f366 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a"><a class="image-enlarge-link" data-lightbox="image_c88a" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/_jcr_content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/backgroundimg.img.jpg/1689357391116.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.high.jpg/1689357391090.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.480.high.jpg/1689357391090.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.full.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.480.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.medium.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.620.high.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.320.low.jpg/1689357391090.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a2/tab/image_c88a/foregroundimg.img.480.medium.jpg/1689357391090.jpg" alt='Example data from Applied Biosystems Transcriptome Analysis Console Software' title='Example gene expression data'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a img{ width:100%; max-width: 500px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a2tabimage_c88a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><b>Step 5. Data Analysis:</b> Analyze data using the <a href="/sg/en/home/digital-science/thermo-fisher-connect/all-analysis-modules.html">Thermo Fisher Connect cloud-based QuantiGene data analysis</a> application from any computer, anywhere. For advanced analysis and visualization, data can be analyzed using the free desktop application software, <a href="/sg/en/home/life-science/microarray-analysis/">Applied Biosystems Transcriptome Analysis Console (TAC) software</a>. TAC provides visualization and interpretation using tools such as scatter and volcano plots, hierarchical clustering, and provides link outs to publicly available annotations.</p></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> 384-well assay </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-eb5a3"><div class="container-eb5a3 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2 id="quantigenePHTA"> QuantiGene Plex high throughput assays </h2></div><div class="text parbase section"><p>The QuantiGene Plex 384-well assay format enables multiplex gene expression analysis in a high throughput format. The assay uses target-specific probes to capture the RNA of interest and branched DNA technology to amplify fluorescent signals which are read with the <a href="/sg/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/luminex-instruments.html">FLEXMAP 3D</a>, Luminex platform. Automation and batch processing options for this assay format increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects.</p><p>The <a href="/order/catalog/product/QP1016">QuantiGene Plex 384-well Assay Kit</a> (Cat. No. QP1016) contains all the necessary reagents, buffers, and plates to perform QuantiGene Plex assays in 384-well plates. Design your target-specific QuantiGene Plex panel with our <a href="/order/quantigene-plex/configuration">Panel Configuration Tool</a> or <a href="/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html">contact</a> your local Technical Sales Specialist for more information.<br>  </p></div><div class="heading section"><h2> QuantiGene Plex 384-well example data </h2></div><div class="text parbase section"><p>The QuantiGene Plex 384-well assay is designed to increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects. Below is example data that was generated using the 384-well format using the recommended equipment listed below.</p><p><b>Stimulation of cytokine production in HeLa cells</b><br> Cytokine production in cultured HeLa cells was stimulated using a Cell Stimulation Cocktail and monitored for various time points up to 24 hours. In this experiment, a full 80-plex panel was used for screening purposes focused on cytokine, and chemokine biomarkers.</p></div><div class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb"><a class="image-enlarge-link" data-lightbox="image_eccb" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/_jcr_content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/backgroundimg.img.jpg/1689357390546.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.low.jpg/1689357390524.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.medium.jpg/1689357390524.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.480.medium.jpg/1689357390524.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.620.high.jpg/1689357390524.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.full.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.480.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.medium.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.620.high.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.320.low.jpg/1689357390524.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_eccb/foregroundimg.img.480.medium.jpg/1689357390524.jpg" alt='Example data from QuantiGene Plex 384-well assay showing cytokine production stimulation in HeLa cells' title='Example data from QuantiGene Plex 384-well assay'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb img{ width:100%; max-width: 1000px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_eccb .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div class="text parbase section"><p><small><span class="muted"><b>Figure 1. Normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.</b> Y-axis: Average normalized expression; x-axis: mRNA targets; z-axis: time points of stimulation. Only a subset of gene targets tested is being displayed. HeLa cells were cultured in a 384-well format and stimulated with a Cell Stimulation cocktail for various times up to 24h (1h, 2h, 4h, 24h). Unstimulated cells served as baseline control. At the indicated time points, cells were lysed according to the <a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017267_QuantiGene_Cultured_Cells_PI.pdf" target="_blank">QuantiGene sample prep protocol</a> for cell lysates using liquid handling (<a href="/order/catalog/product/650-INSTR">Thermo Fisher Platemate</a>). After all samples from all time points were prepared, the QuantiGene Plex 384-well assay was performed. Chart illustrates the normalized gene expression results.</span></small></p></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>The normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail reveals the degree of up- or downregulation. The average normalized expression (y-axis) represents the level of gene induction for the mRNA targets listed on the x-axis, while the time points of stimulation are shown on the z-axis. Of note, for example IL1B, IL8 and TGFB1 show pronounced relative gene expression at various time points of stimulation. While TGFB1 relative expression seems to gradually increase over time, peak expression for IL1B is seen at later time points around 24h. Table 1 shows normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.</p></div><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73"><a class="image-enlarge-link" data-lightbox="image_2d73" href="/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/_jcr_content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/backgroundimg.img.jpg/1689357390617.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.low.jpg/1689357390587.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.medium.jpg/1689357390587.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.480.medium.jpg/1689357390587.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.620.high.jpg/1689357390587.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.full.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.480.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.medium.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.620.high.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.320.low.jpg/1689357390587.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/tabworkflowcontainer/container-eb5a3/tab/image_2d73/foregroundimg.img.480.medium.jpg/1689357390587.jpg" alt='Normalized gene expression of various target genes after stimulation of cytokine production in HeLa cells after 1, 2, 4, and 24 hours' title='Normalized gene expression of target genes after stimulation of cytokine production '></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73 img{ width:100%; max-width: 900px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainercontainer-eb5a3tabimage_2d73 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div><div class="text parbase section"><p><small><span class="muted"><b>Table 1 and 2. Normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail. </b></span></small></p></div><div class="heading section"><h3> See how scientists are using QuantiGene Plex 384-well in their research: </h3></div><div class="text parbase section"><ol><li>Danahay et. al. (2015) <a href="https://www.sciencedirect.com/science/article/pii/S2211124714010468" target="_blank">Notch2 Is Required for Inflammatory Cytokine-Driven Goblet Cell Metaplasia in the Lung.</a><span class="icon icon-outbound"></span> Cell Reports 10:239-252.</li><li>Ferrer et al. (2014) <a href="https://pubmed.ncbi.nlm.nih.gov/24873859/" target="_blank">A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.</a><span class="icon icon-outbound"></span> Stem Cells Transl Med 2:911-922.</li><li>Severyn et al. (2016) <a href="https://pubmed.ncbi.nlm.nih.gov/27461835/" target="_blank">Development of a High-Throughput Gene Expression Screen for Modulators of RAS-MAPK Signaling in a Mutant RAS Cellular Context.</a><span class="icon icon-outbound"></span> J Biomol screen 9:989-997.</li><li>Neilsen et al. (2020) <a href="https://www.nature.com/articles/s41597-020-0365-2" target="_blank">High-throughput identification of protein functional similarities using a gene-expression-based siRNA screen</a>. Sci Data 7:27.<br>  </li></ol></div><div class="heading section"><h2> QuantiGene Plex instrumentation and equipment for high throughput and batch processing </h2></div><div class="text parbase section"><p>Find instrumentation and equipment to make every step of your QuantiGene Plex 384-well workflow easy. For detailed options and recommendations for batch processing, refer to the <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019482_QuantigenePlexGeneExpressionAssay_384wells_UG.pdf" target="_blank">QuantiGene Plex 384-well manual</a> or <a href="/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html">contact</a> your local Technical Sales Specialist.</p><div class="table-component-wrapper"><table class="rte-table" id="table-1600283318"><tbody><tr><th>Workflow step</th><th colspan="2">Recommended equipment</th><th>Description</th></tr><tr><td style="width: 20%;"><b>Sample incubation and target hybridization</b></td><td style="border-right: none;">Temperature regulated orbital shaker: <a href="/order/catalog/product/SHKE4450">MaxQ 4450 Benchtop Orbital Shakers</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/maxq-shaker.jpg" alt="MaxQ 4450 Benchtop Orbital Shakers" height="95"></td><td style="width: 40%;">Versatile shaker ideal for incubating a small number of vessels and cell culture and many other applications.</td></tr><tr><td><b>Plate washes</b></td><td colspan="2">Magnetic microplate washers</td><td>Automated magnetic microplate washer for 384-well plates.</td></tr><tr><td><b>Sample incubation</b></td><td style="border-right: none;">Plate sealer: <a href="/order/catalog/product/AB-1443A">ALPS 50 V-Manual Heat Sealer</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/alps-heat-sealer.jpg" alt="Thermo Scientific ALPS 50 V- Manual Heat Sealer" height="95"></td><td>Applies consistent, secure, tight seals around individual wells, eliminating sample loss through evaporation and cross contamination between wells.</td></tr><tr><td><b>Sample and plate additions</b></td><td style="border-right: none;"><a href="/order/catalog/product/650-INSTR">Versette Automated Liquid Handler</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/versette-handler.jpg" alt="Thermo Scientific Versette Automated Liquid Handler platform" height="95"></td><td>Compact liquid handler featuring user-friendly programming, reliable performance, and a choice of 96- or 384-channel pipetting heads.</td></tr><tr><td><b>Multiplex detection</b></td><td style="border-right: none;"><a href="/order/catalog/product/APX1342">Luminex FLEXMAP 3D Instrument System</a></td><td style="border-left: none;"><img src="/content/dam/LifeTech/global/life-sciences/GeneExpressionAnalysisGenotyping/Images/2020/luminex-instrument.jpg" width="95" alt="Luminex FLEXMAP 3D Instrument System"></td><td>Most advanced and multifunctional multiplex unit for simultaneous measurement of up to 80 tests in a single reaction volume.</td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainer" href="#collapse4"><span class="caret"></span><strong> Specification and FAQs </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-eb5a4"><div class="container-eb5a4 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> QuantiGene Plex specifications </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--693642153"><tbody><tr><td style="border: none;"> </td><th style="width: 28%;">Parameter</th><th style="width: 28%;">96-well</th><th style="width: 28%;">384-well</th></tr><tr><td style="background-color: #f3f3f3;" rowspan="6"><b>Product specification</b></td><td><b>Sensitivity (Limit of detection)</b></td><td>1,000–2,000 transcripts/assay well</td><td>2,000–4,000 transcripts/assay well</td></tr><tr><td><b>Assay variation (C.V.)</b></td><td>≤15%</td><td>≤20%</td></tr><tr><td><b>Linearity</b></td><td colspan="2">≥3 logarithmic units</td></tr><tr><td><b>Plex level</b></td><td>3–80</td><td>3–80</td></tr><tr><td><b>Samples</b></td><td colspan="2">Cultured cells, bacteria, whole blood, PAXgene blood or dried blood spots, fresh/frozen tissues (animal or plant), FFPE samples, purified RNA</td></tr><tr><td><b>Compatible Luminex instrumentation</b></td><td>MAGPIX, Luminex 200, FLEXMAP 3D, INTELLIFLEX</td><td>FLEXMAP 3D, INTELLIFLEX</td></tr><tr><td style="background-color: #f3f3f3;" rowspan="5"><b>Assay volumes</b></td><td><b>Target probe hybridization</b></td><td>100 µL</td><td>70 µL</td></tr><tr><td><b>Pre-amp hybridization</b></td><td>100 µL</td><td>30 µL</td></tr><tr><td><b>Amp hybridization</b></td><td>100 µL</td><td>30 µL</td></tr><tr><td><b>Label probe hybridization</b></td><td>100 µL</td><td>30 µL</td></tr><tr><td><b>SAPE</b></td><td>100 µL</td><td>30 µL</td></tr></tbody></table></div></div><div class="heading section"><h2> QuantiGene Plex FAQs </h2></div><div class="text parbase section"><ol><li><b>What sample types are compatible with QuantiGene Plex assay? <br></b>Whole blood, fresh tissue, cultured cells, and purified RNA are validated. Other sample types including Tempus blood, bacteria, H&E stained/unstained FFPE, and blood spots should also be applicable.</li><li><b>What data analysis software solutions are offered for the QuantiGene Plex assay?<br></b>Analyze data using the <a href="https://apps.thermofisher.com/apps/quantigene/">QuantiGene cloud-based application</a> from any computer, anywhere. For advanced analysis and visualize, data can be analyzed using the free desktop application software, <a href="/sg/en/home/life-science/microarray-analysis/">Applied Biosystems Transcriptome Analysis Console (TAC) software</a>.</li><li><b>Can the VorTemp™ 56 Shaking Incubator be used for hybridization?<br></b>We highly recommend the MaxQ Shaking incubator for optimal assay performance. It is possible to use the Vortemp, but 800 rpm is recommended.</li><li><b>What is the recommended temperature for hybridization?<br></b>The Day 1 temperature is 54°C while the Day 2 temperature is 50°C. Please use Quantigene Incubator Temperature Validation Kit (QS0517) to verify the incubator temperature.</li><li><b>What are the proper storage conditions for the QuantiGene Plex reagents?<br></b>When the kit arrives, please follow the instructions to store individual components. The Preamp/Amp/LP Diluent, Bead Mix, SAPE, SAPE Diluent should be stored at 4°C, while probe set mix, Proteinase K, and blocking reagent should be stored at -20°C. Others are stored at room temperature. Refer to the product insert for details.</li><li><b>What reagents are included in the kit?<br></b>The kit provides all necessary reagents to perform the assay except Target Probe and Bead mix which are ordered separately. See the user manual for a list of the reagents.</li><li><b>Can QuantiGene Plex 384-well assays be used for miRNA or DNA analysis?<br></b>No. We have options with the QuantiGene Singleplex assay for miRNA and DNA.</li><li><b>Should any control/housekeeping genes be used in my panel<br></b>Three or more control/housekeeping genes are recommended to normalize the gene expression results.</li><li><b>Can I use a heat sealer to seal the plate on day 2 of the assay<br></b>No, manual sealing using the provided seal is recommended. Heat sealing would deform the plate causing problem in washing.</li><li><b>How do I dilute my samples?<br></b>For cell lysate or blood lysate, please use Diluted Lysis Mix (DLM); for tissue homogenates, please use Homogenizing Solution.</li><li><b>How should I seal the Day 1 plate for Target Probe hybridization?<br></b>The Day 1 plate can be sealed either manually or using a heat sealer. The pressure seal for manual sealing and the heat seal AB-0757 for Thermo Fisher ALPS 50 V (AB-1443A) are provided in the kit. If a different heat sealer will be used, please consult the manufacturer for suitable sealing.</li></ol></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsystabworkflowcontainer" href="#collapse5"><span class="caret"></span><strong> Resources </strong></a></div><div id="collapse5" class="accordion-body collapse "><div class="accordion-inner container-eb5a5"><div class="container-eb5a5 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Technical guides </h3></div><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/factors-affecting-blood-gene-expression-app-note.pdf" target="_blank">Factors affecting blood gene expression</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/LSG/brochures/defining-quantigene-plex-assay-panel-app-note.pdf" target="_blank">Defining a QuantiGene Plex panel</a></li></ul></div><div class="heading section"><h3> User manuals </h3></div><div class="text parbase section"><p>User manuals for use with assay kits QP1013, QP1014, QP1015 and QP1016</p><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/MAN0017862_quantigene-plex-gene-expression-assay-user-guide.pdf" target="_blank">QuantiGene Plex manual 3-64 plex and 65-80 plex assays</a></li><li><span class="icon icon-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019482_QuantigenePlexGeneExpressionAssay_384wells_UG.pdf" target="_blank">QuantiGene Plex 384-well assays</a></li></ul><p>QuantiGene sample processing</p><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf" target="_blank">Preparation of bacterial homogenates for use in QuantiGene Assays</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017267_QuantiGene_Cultured_Cells_PI.pdf" target="_blank">Cultured cells</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017268_QuantiGene_Fresh_Frozen_Tissues_PI.pdf" target="_blank">Fresh/frozen animal or plant tissues</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017265_QuantiGene_Blood_PI.pdf" target="_blank">Blood samples</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/manuals/MAN0017266_QuantiGene_FFPE%20Tissues_PI.pdf" target="_blank">FFPE tissue samples</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/LSG/manuals/QG-Incubator-Temp-Validation-Kit_32016.pdf" target="_blank">QuantiGene incubator temperature validation kit</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/LSG/brochures/Data_Sheet_MS_3_digital.pdf" target="_blank">IKA plate shaker</a></li></ul></div><div class="heading section"><h3> Application notes </h3></div><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/quantigene-assays-app-note.pdf" target="_blank">QuantiGene Application Guide</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/determination-gene-signatures-subgroup-melanoma-dna-hybridization-assays-app-note.pdf" target="_blank">New determination of gene signatures to subgroup melanoma patients using novel branched DNA hybridization assays</a></li><li><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/transcript-regulation-prototypical-inducers-human-hepatocytes-app-note.pdf" target="_blank">Transcript regulation of 18 ADME genes by prototypical inducers in human hepatocytes using QuantiGene Plex Assays</a></li></ul></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="quantigene-plex"> QuantiGene Plex Assay services </h2></div><div class="text parbase section"><h3>Let us test and analyze your samples to help save you time </h3><p>Accelerate your research by outsourcing your gene expression profiling projects to our in-house Madison, WI scientists. Our assay service is designed to help you meet your specific project goals and timelines so you can free up your valuable resources for other discovery research. </p><p><b>Assay services features and benefits: <br> Design support</b>—save time by accessing our bioinformatics team to customize your probe and panel design; obtain high-quality data with a fast turnaround time<br><b>Consistency</b>—use the same customized assays in your follow-up studies that our team uses in the service<br><b>Flexibility</b>—sample types include purified RNA, cultured cells, or tissue (whole blood, fresh or frozen tissue, or FFPE) pre-lysed in Invitrogen QuantiGene Buffer<br><b>Choice</b>—a comprehensive list of target areas, including immunology, inflammation, cancer signalling, toxicology, neurology, cardiology, bone biology, metabolism, endocrinology, stem cell biology, apoptosis, and autophagy</p><p><a href="/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html"><span class="btn btn-primary">Request a quote</span></a></p></div><div class="well-cmp section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_b659uiparimage_60c3"><a href="http://assets.thermofisher.com/TFS-Assets/LSG/brochures/defining-quantigene-plex-assay-panel-app-note.pdf" target='_blank'><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.full.high.jpg/1689357391255.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.full.high.jpg/1689357391255.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.320.high.jpg/1689357391255.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.480.high.jpg/1689357391255.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.320.medium.jpg/1689357391255.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.620.high.jpg/1689357391255.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.320.low.jpg/1689357391255.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_b659/uipar/image_60c3/foregroundimg.img.480.medium.jpg/1689357391255.jpg" alt='QuantiGene Plex Assay Panel Designing Guide Cover page' title='QuantiGene Plex Assay Panel Designing Guide'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_b659uiparimage_60c3 img{ width:100%; max-width: 231px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_b659uiparimage_60c3 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_b659uiparimage_60c3 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><h3>Guide to designing custom panels</h3><p>Learn how to design a custom Invitrogen QuantiGene Plex Assay Panel for your research needs. This application note details recommendations for housekeeping genes and how to get started.</p><p><a class="cta-link" href="http://assets.thermofisher.com/TFS-Assets/LSG/brochures/defining-quantigene-plex-assay-panel-app-note.pdf" target="_blank">Download article</a></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="heading section"><h2> Related products and resources </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><h3> Instrumentation and software </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_e841uiparimage_ecbe"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.320.medium.jpg/1689357391353.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.480.high.jpg/1689357391353.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.620.high.jpg/1689357391353.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.full.high.jpg/1689357391353.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.320.high.jpg/1689357391353.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.480.high.jpg/1689357391353.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.320.medium.jpg/1689357391353.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.620.high.jpg/1689357391353.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.320.low.jpg/1689357391353.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_e841/uipar/image_ecbe/foregroundimg.img.480.medium.jpg/1689357391353.jpg" alt='Luminex instrument' title='Luminex 200 instrument'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_e841uiparimage_ecbe img{ width:100%; max-width: 650px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_e841uiparimage_ecbe .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_e841uiparimage_ecbe .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><h4><a href="/sg/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/luminex-instruments.html">Luminex Instruments</a></h4><p>Explore Luminex systems: MAGPIX, Luminex 200, and FLEXMAP3D.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_829duiparimage_6ead"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.320.medium.jpg/1689357391429.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.480.high.jpg/1689357391429.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.620.high.jpg/1689357391429.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.full.high.jpg/1689357391429.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.320.high.jpg/1689357391429.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.480.high.jpg/1689357391429.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.320.medium.jpg/1689357391429.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.620.high.jpg/1689357391429.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.320.low.jpg/1689357391429.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_829d/uipar/image_6ead/foregroundimg.img.480.medium.jpg/1689357391429.jpg" alt='QuantiGene Plex Cloud-based Software icon' title='QuantiGene Plex Software icon'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_829duiparimage_6ead img{ width:100%; max-width: 650px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_829duiparimage_6ead .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_829duiparimage_6ead .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><h4><a href="/sg/en/home/digital-science/thermo-fisher-connect/all-analysis-modules.html">QuantiGene Plex software</a></h4><p>Learn about data analysis and visualization software.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><h3> Gene expression assays </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_af6buiparimage_ba2b"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.320.medium.jpg/1689357391517.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.480.high.jpg/1689357391517.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.620.high.jpg/1689357391517.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.full.high.jpg/1689357391517.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.320.high.jpg/1689357391517.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.480.high.jpg/1689357391517.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.320.medium.jpg/1689357391517.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.620.high.jpg/1689357391517.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.320.low.jpg/1689357391517.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_af6b/uipar/image_ba2b/foregroundimg.img.480.medium.jpg/1689357391517.jpg" alt='GeneChip Expression profiling microarray for studying transcriptome-wide gene expression profiling ' title='GeneChip Microarray chip'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_af6buiparimage_ba2b img{ width:100%; max-width: 650px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_af6buiparimage_ba2b .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_af6buiparimage_ba2b .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><h4><a href="/sg/en/home/life-science/microarray-analysis/transcriptome-profiling-microarrays.html">Microarrays</a></h4><p>Microarray platform for fast, detailed transcriptome-wide expression profiling.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_a932uiparimage_e840"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.320.medium.jpg/1689357391594.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.480.high.jpg/1689357391594.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.620.high.jpg/1689357391594.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.full.high.jpg/1689357391594.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.320.high.jpg/1689357391594.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.480.high.jpg/1689357391594.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.320.medium.jpg/1689357391594.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.620.high.jpg/1689357391594.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.320.low.jpg/1689357391594.jpg,https://www.thermofisher.com/sg/en/home/life-science/gene-expression-analysis-genotyping/quantigene-rna-assays/quantigene-plex-assay/jcr:content/MainParsys/well_a932/uipar/image_e840/foregroundimg.img.480.medium.jpg/1689357391594.jpg" alt='Artist rendition of real-time PCR data' title='Artist rendition of real-time PCR data'></picture><style> #cq-image-jsp-8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_a932uiparimage_e840 img{ width:100%; max-width: 650px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_a932uiparimage_e840 .image-contain" + "," + "#cq-image-jsp-"+"8c2886f2-b3fd-41fe-9f3b-1558488d55c5MainParsyswell_a932uiparimage_e840 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><h4><a href="/sg/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/gene-expression-using-real-time-pcr.html">Real-time PCR</a></h4><p>TaqMan Gene Expression Assays, master mixes and instrumentation.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div><div style="clear:both"></div><div class="LTRawHTML section"><style> .modal-backdrop { opacity: 0.1; 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