Preparation of Gramine Nanosuspension and Protective Effect of Gramine on Human Oral Cell Lines by Induction of Apoptosis

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Nanosuspension and Protective Effect of Gramine on Human Oral Cell Lines by Induction of Apoptosis</title> <meta name="description" content="Preparation of Gramine Nanosuspension and Protective Effect of Gramine on Human Oral Cell Lines by Induction of Apoptosis"> <meta name="keywords" content="apoptosis, HEp-2 cell line, KB cell line mitochondria, gramine, nanosuspension"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a 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87409</div> </div> </div> </div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Preparation of Gramine Nanosuspension and Protective Effect of Gramine on Human Oral Cell Lines by Induction of Apoptosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Suresh">K. Suresh</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Arunkumar"> R. Arunkumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of this study is to investigate the preparation of gramine nano suspension and protective effect of Gramine on the apoptosis of laryngeal cancer cells cell line (HEp-2 and KB). The growth inhibition rate of Hep-2 and KB cells in vitro were measured by MTT assay and apoptosis by, levels of reactive oxygen species, mitochondrial membrane potential, morphological changes and flowcytometry. Based on the results, we determined the effective doses of gramine as 127.23碌m/ml for 24 hr and 119.81 碌m/ml for 48hr in hep-2 cell line and 147.58 碌m ml for 24 hr and 123.74碌m 碌m/ml for 48hr in KB cell line. cytotoxicity effects of gramine were confirmed by treatment of HEp-2 cell and KB cell with IC50 concentration of gramine resulted in sequences of events marked by the enhance the apoptosis accompanied by loss of cell viability, modulation of reactive oxygen species and cell cycle arrest through the induction of G0/G1 phase arrest on HEp-2 cells. Our study suggests that the nanosuspension of gramine possesses the more cytotoxic effect of cancer cells and a novel candidate for cancer chemoprevention. <iframe src="https://publications.waset.org/abstracts/21324.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title="apoptosis">apoptosis</a>, <a href="https://publications.waset.org/abstracts/search?q=HEp-2%20cell%20line" title=" HEp-2 cell line"> HEp-2 cell line</a>, <a href="https://publications.waset.org/abstracts/search?q=KB%20cell%20line%20mitochondria" title=" KB cell line mitochondria"> KB cell line mitochondria</a>, <a href="https://publications.waset.org/abstracts/search?q=gramine" title=" gramine"> gramine</a>, <a href="https://publications.waset.org/abstracts/search?q=nanosuspension" title=" nanosuspension"> nanosuspension</a> </p> <a href="https://publications.waset.org/abstracts/21324/preparation-of-gramine-nanosuspension-and-protective-effect-of-gramine-on-human-oral-cell-lines-by-induction-of-apoptosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21324.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">453</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational 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Preparation of Gramine Nanosuspension and Protective Effect of Gramine on Human Oral Cell Lines by Induction of Apoptosis