High-Throughput Genotyping | CRISPR Validation

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} .ngs-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } .ngs-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .ngs-new-container { text-align: center; margin-top: 0; } .ngs-new-content { width: 90%; margin: 0 auto; } .ngs-new-content { display: inline-block; } #ngs-content-box-right, #ngs-content-box-left { width: 100%; text-align: center; } #ngs-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .ngs-new-content { width: 90%; margin: 0 auto; } .ngs-content-box img { width: 350px; } } </style> <div class="ngs-new-container"> <div class="ngs-new-content"> <div class="ngs-content-box" id="ngs-content-box-left"> <h1>High-Throughput Genotyping (CRISPR Validation)</h1> <div> </div> <p>High-throughput genotyping is a next generation sequencing (NGS)-based assay ideal for ultra-sensitive, high-throughput, sample-to-answer genotyping projects, particularly to validate targeted CRISPR gene editing experiments. Advancements in genome editing techniques, namely CRISPR/Cas9, have greatly facilitated cell line engineering, gene targeting, and the development of transgenic models. However, accurately identifying correctly targeted cells remains a significant bottleneck for large-scale projects.</p> <p>Our proprietary, high-throughput genotyping service, genoTYPER-NEXT<sup>™</sup>, is a more effective and efficient approach than T7E1, TIDE, and IDAA assays for screening targeted cell lines. We also offer targeted, whole genome, and amplicon NGS solutions to aid on- and off-target analysis of genome editing.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry?utm_source=crispr-validation" target="_blank"><button>Request Quote</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02l4pYk0" target="_blank"><img alt="" src="/-/media/10013-D_Customer-Referral_WebBanRight2.ashx?la=en&hash=21BD9C4C09D7AFE28645FBB92782B71F584DE6BE" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <h3>Enhancing Gene Transfer Efficiency:<br /> Viral vs. Non-Viral Methods</h3> <p>Friday, September 20, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02Jnzck0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <p> </p> <h2>What is high-throughput genotyping?</h2> <p>High-throughput genotyping is the rapid analysis of genetic markers or gene variations in a sample, often with tens to hundreds of samples simultaneously. This method is a valuable tool for researchers conducting genome-wide association studies (GWAS), population genetics studies, pharmacogenomics, and research involving genetic risk factors and responses to drug therapies.</p> <p> </p> <h2>How do you validate CRISPR gene editing?</h2> <p>CRISPR gene editing is validated by polymerase chain reaction (PCR), Sanger sequencing, or NGS. These techniques are used to identify and verify gene modifications within the edited DNA sample.</p> <p> </p> <h2>What is the target validation of CRISPR-Cas9?</h2> <p>The target validation of CRISPR-Cas9 is the confirmation of selected guide RNA (gRNA) sequences and its specificity to the gene of interest. This method involves analyzing other genomic loci that share sequence similarity with the chosen gRNA and comparing it to the non-targeted controls (gRNA targeting a non-functional region) to ensure that the gRNA predominantly targets the intended genomic locus. The functional consequences of gene editing,such gene knockout and subsequent absence of protein expression, are confirmed and quantified via quantitative PCR (qPCR) or deep sequencing.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .main-container { padding: 50px 0; } .workflow-container { width: 95%; margin: 0 auto; margin-top: 40px; } .workflow { width: 100%; margin: 0 auto; text-align: center; } .workflow-step { display: inline-block; width: 24%; vertical-align: top; padding: 5px; margin-bottom: 40px; } .workflow-step img { width: 150px; margin: 0 auto; margin-bottom: 40px; } .workflow-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .workflow-step p { width: 80%; margin: 0 auto; text-align: center; } .workflow-icon { text-align: center; } @media only screen and (max-width: 1200px) { .workflow { text-align: left; margin: 0 auto; } .workflow-step { width: 30%; } } @media only screen and (max-width: 500px) { .workflow-step { width: 100%; } } </style> <div class="main-container"> <h2 style="text-align: center; margin-bottom: 30px;">genoTYPER-NEXT: HIGH-THROUGHPUT ASSAY FOR CRISPR VALIDATION</h2> <div class="workflow-container"> <div class="workflow"> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Icon_210x110_SubmitSamples.ashx?la=en&hash=EFFC6B695DC2332F2946270CDEEB487CE81851FD" /> </div> <h3>1. SUBMIT SAMPLES</h3> <p>Submit CRISPR-edited cell lines in 96-well plates</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Icon_210x110_Pre-Mixed-Tube.ashx?la=en&hash=6B47835CAC8B013590C9554F757464753444739F" /> </div> <h3>2. AMPLIFY ON/OFF-TARGET SITE(S)</h3> <p>Cells lysis and PCR with barcoded primers</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Icon_210x110_Sequence.ashx?la=en&hash=F840DDDB61C02B6F2C0C5C024690F1D3E5C88BA2" /> </div> <h3>3. SEQUENCE</h3> <p>Samples pooled and sequenced on Illumina® platform</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/RegisterAccount/Login?returnUrl=https://clims4.genewiz.com//OrderResult/NGSSampleReport/2" target="_blank"><img alt="" src="/-/media/Images/Icon_210x110_Analyze.ashx?la=en&hash=3D16B45953DE6B392D47694553F32717ACC623D0" /></a> </div> <h3>4. ANALYZE DATA INTERACTIVELY</h3> <p><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/RegisterAccount/Login?returnUrl=https://clims4.genewiz.com//OrderResult/NGSSampleReport/2" target="_blank">Visualize results</a> (login required) in genoTYPER-NEXT browser</p> </div> </div> </div> <h3><span style="font-size: 24px;">ADDITIONAL NGS SERVICES FOR CRISPR VALIDATION</span></h3> <a rel="noopener noreferrer" href="#crispr-add-services"><button><span style="font-size: 16px;"><strong>View CRISPR Validation Services</strong></span></button></a> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; 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rel="noopener noreferrer" href="https://hubs.ly/Q02gBwzs0" target="_blank"><button style="text-align:left;">LEARN MORE</button></a> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Gray margin-top-60 margin-bottom-0 padding-top-60 padding-bottom-60'> <div class="container"> <div class="headline-center"> <h1>FEATURES & BENEFITS</h1> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Automated, high-throughput workflow</strong> – Up to 10,000 samples per run </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Hassle-free</strong> - Avoid gDNA extraction, pre-screening prior to sequencing, and TA cloning </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Highly sensitive</strong> – Detection of <1% allele frequency, full INDEL resolution, and frameshift analysis </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <div style="background: #f1f0f0;padding-bottom: 30px;position:relative; z-index:9;"> More reasons to <a href="https://web.genewiz.com/why-genewiz?_ga=2.258573833.20658592.1665665586-1790848659.1652957837" target="_blank">choose </a>Azenta</div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-60 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>Technical Resources</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Prepare_Samples_Web-Icon-Workflow_Purple.ashx?h=91&la=en&w=91&hash=46CD2A5C3BAF8BA51A6B26A29368BBB9171BDA5C" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Sample Submission 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class="tech-resources-main"> <div class="tech-resources-container"> <div class="tech-resource"> <a id="crispr-tech-resources" class="anchor"></a> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13035_CRISPR-Validation_CS-Thumbnail-(1).ashx?la=en&hash=EFC94455EB4D3426BD0A5296530D9EEE591BA3BC" /> </div> <div class="resource resource-right"> <h3>Case Study | Rapid, Scalable, and Ultra-Sensitive Validation of Allele-Specific Gene Editing Events with genoTYPER-NEXT™</h3> <p>Discover the powerful capabilities of genoTYPER-NEXT in this illuminating case study and learn how this high-throughput assay allows rapid and accurate genotyping of CRISPR-edited cell lines. <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/case-study/high-throughput-genotyping" target="_blank"><button>Download</button></a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/73025-D_CRISPR-Validation_Webinar-Thumb.ashx?la=en&hash=B871BB238DE9407A49AF25584D7A3E400E02ADEA" width="220" /> </div> <div class="resource resource-right"> <h3>Webinar | Rapid and Sensitive Validation of Gene Editing with Novel NGS Assays</h3> <p>Learn about current techniques to validate gene edits and how recent advancements in next generation sequencing are improving the sensitivity and scalability of screening genomic manipulations. <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/webinar_novelngsassays" target="_blank"><button>Watch</button></a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/MG_qPCR_Blog_US_Web-Tech-Thumb_Blue.ashx?la=en&hash=2A4957D1C2685CDC902644346D94E592804711C4" /> </div> <div class="resource resource-right"> <h3>Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide</h3> <p>This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making. <br /> <a href="https://blog.genewiz.com/ngs-qpcr-or-sanger-sequencing-an-assay-selection-guide?utm_campaign=2022-03 GEN MG-qPCR&utm_source=qPCR&utm_medium=technical resources" target="_blank"> <button>Read Blog</button> </a> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Illumina"><img alt="Illumina" src="/-/media/hires-illumina-logo-rgb_new.ashx?w=200&la=en&hash=2DE7778E5877DA9F3D063A9FAF021DA575ECF223" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#PacBio"><img alt="PacBio" src="/-/media/PacBio-CSP_logo.ashx?w=200&la=en&hash=1568DC13B370C032463997ED7BC5591C1FECF672" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#10x"><img alt="10x Genomics" src="/-/media/10x-Genomics-CSP_logo.ashx?w=200&la=en&hash=2584354CF0810D46E980E04C8C03461D332EE516" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#Nanostring"><img alt="NanoString" src="/-/media/Images/Resources/NGS-Platforms-and-Specifications/NanoString_resized_logo.ashx?w=200&la=en&hash=A22CDFCF6A16A9884C633E8C2EF98B468325D130" style="width: 200px;" /></a> </div> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Oxford-Nanopore"><img alt="Nanopore" 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href='/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/targeted-resequencing_110x210_Purple.ashx?h=110&la=en&w=210&hash=8989CF2EA77919B6CB23CDDF96B918523189B338" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Custom Targeted Sequencing</h3><br> </div> <div class="row"> <span>Primer design, amplification, and sequencing of edited and predicted off-target sites</span> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img 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