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Dai</title> <meta name="description" content="Search results for: Xiaowei Dai"> <meta name="keywords" content="Xiaowei Dai"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Xiaowei Dai"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 8</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Xiaowei Dai</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Media Façades in the Wild: Some Lessons</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hai-Ning%20Liang">Hai-Ning Liang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Dai"> Xiaowei Dai</a>, <a href="https://publications.waset.org/abstracts/search?q=Nancy%20Diniz"> Nancy Diniz</a>, <a href="https://publications.waset.org/abstracts/search?q=Charles%20Fleming"> Charles Fleming</a>, <a href="https://publications.waset.org/abstracts/search?q=Woon%20Kian%20Chong"> Woon Kian Chong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Media displays in public areas are becoming increasingly pervasive—they are used in many settings, come in different sizes, serve different purposes, and have varied degrees of interactivity. In this paper, we aim to provide a survey of how these displays, often named media façades, are used in the wild in a city in China which is undergoing a rapid growth. This survey is intended to raise greater awareness and discussion about the use and effect of these displays in public areas. Through this survey, we have been able to distill some lessons of what is good, bad, and ugly about some current examples of media displays used in a city that is transitioning into becoming a modern one and one that is located in one of the fastest growing areas in Asia. With this research, we hope that we can provide technology designers and architects with some general principles that can help them integrate these types of technologies into their architectural creations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=large%20displays" title="large displays">large displays</a>, <a href="https://publications.waset.org/abstracts/search?q=media%20fa%C3%A7ades" title=" media façades"> media façades</a>, <a href="https://publications.waset.org/abstracts/search?q=interaction%20design" title=" interaction design"> interaction design</a>, <a href="https://publications.waset.org/abstracts/search?q=architectural%20displays" title=" architectural displays"> architectural displays</a> </p> <a href="https://publications.waset.org/abstracts/10362/media-facades-in-the-wild-some-lessons" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10362.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">402</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Numerical Investigation of Slot Die Coating Based on VOF Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhidi%20Lei">Zhidi Lei</a>, <a href="https://publications.waset.org/abstracts/search?q=Xixi%20Cai"> Xixi Cai</a>, <a href="https://publications.waset.org/abstracts/search?q=Jue%20Ding"> Jue Ding</a>, <a href="https://publications.waset.org/abstracts/search?q=Peifen%20Weng"> Peifen Weng</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Li"> Xiaowei Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the process of preparing thin films by chemical solution method, the uniformity of gel coating has a great influence on the subsequent film thickness. Based on a coating device, the research tracks the interface development of gas-liquid flow by volume of fluid method (VOF). The effects of fluid viscosity and wall wetting property for the shape and position of the coating window are discussed in the process of slot die coating. The result shows that downstream contact lines gets closer to the corner with the increase of fluid viscosity. When the viscosity increases from 0.2Pa∙s to 0.3Pa∙s, 18.2% of the vortex region area will be reduced. With the static contact angle of upper die head surface (θ_sd) increasing, X_u decreased gradually which cause the instability changes of upstream surface. Also, θ_sd increasing brings the reduction of vortex region. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=film%20growth" title="film growth">film growth</a>, <a href="https://publications.waset.org/abstracts/search?q=vortex" title=" vortex"> vortex</a>, <a href="https://publications.waset.org/abstracts/search?q=VOF" title=" VOF"> VOF</a>, <a href="https://publications.waset.org/abstracts/search?q=slot%20die%20coating" title=" slot die coating"> slot die coating</a> </p> <a href="https://publications.waset.org/abstracts/27342/numerical-investigation-of-slot-die-coating-based-on-vof-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27342.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">373</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Dynamical Characteristics of Interaction between Water Droplet and Aerosol Particle in Dedusting Technology </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ding%20Jue">Ding Jue</a>, <a href="https://publications.waset.org/abstracts/search?q=Li%20Jiahua"> Li Jiahua</a>, <a href="https://publications.waset.org/abstracts/search?q=Lei%20Zhidi"> Lei Zhidi</a>, <a href="https://publications.waset.org/abstracts/search?q=Weng%20Peifen"> Weng Peifen</a>, <a href="https://publications.waset.org/abstracts/search?q=Li%20Xiaowei"> Li Xiaowei</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With the rapid development of national modern industry, people begin to pay attention to environmental pollution and harm caused by industrial dust. Based on above, a numerical study on the dedusting technology of industrial environment was conducted. The dynamic models of multicomponent particles collision and coagulation, breakage and deposition are developed, and the interaction of water droplet and aerosol particle in 2-Dimension flow field was researched by Eulerian-Lagrangian method and Multi-Monte Carlo method. The effects of the droplet scale, movement speed of droplet and the flow field structure on scavenging efficiency were analyzed. The results show that under the certain condition, 30μm of droplet has the best scavenging efficiency. At the initial speed 1m/s of droplets, droplets and aerosol particles have more time to interact, so it has a better scavenging efficiency for the particle. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=water%20droplet" title="water droplet">water droplet</a>, <a href="https://publications.waset.org/abstracts/search?q=aerosol%20particle" title=" aerosol particle"> aerosol particle</a>, <a href="https://publications.waset.org/abstracts/search?q=collision%20and%20coagulation" title=" collision and coagulation"> collision and coagulation</a>, <a href="https://publications.waset.org/abstracts/search?q=multi-monte%20carlo%20method" title=" multi-monte carlo method"> multi-monte carlo method</a> </p> <a href="https://publications.waset.org/abstracts/27344/dynamical-characteristics-of-interaction-between-water-droplet-and-aerosol-particle-in-dedusting-technology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27344.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">307</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Achieving Shear Wave Elastography by a Three-element Probe for Wearable Human-machine Interface</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jipeng%20Yan">Jipeng Yan</a>, <a href="https://publications.waset.org/abstracts/search?q=Xingchen%20Yang"> Xingchen Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Zhou"> Xiaowei Zhou</a>, <a href="https://publications.waset.org/abstracts/search?q=Mengxing%20Tang"> Mengxing Tang</a>, <a href="https://publications.waset.org/abstracts/search?q=Honghai%20Liu"> Honghai Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Shear elastic modulus of skeletal muscles can be obtained by shear wave elastography (SWE) and has been linearly related to muscle force. However, SWE is currently implemented using array probes. Price and volumes of these probes and their driving equipment prevent SWE from being used in wearable human-machine interfaces (HMI). Moreover, beamforming processing for array probes reduces the real-time performance. To achieve SWE by wearable HMIs, a customized three-element probe is adopted in this work, with one element for acoustic radiation force generation and the others for shear wave tracking. In-phase quadrature demodulation and 2D autocorrelation are adopted to estimate velocities of tissues on the sound beams of the latter two elements. Shear wave speeds are calculated by phase shift between the tissue velocities. Three agar phantoms with different elasticities were made by changing the weights of agar. Values of the shear elastic modulus of the phantoms were measured as 8.98, 23.06 and 36.74 kPa at a depth of 7.5 mm respectively. This work verifies the feasibility of measuring shear elastic modulus by wearable devices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=shear%20elastic%20modulus" title="shear elastic modulus">shear elastic modulus</a>, <a href="https://publications.waset.org/abstracts/search?q=skeletal%20muscle" title=" skeletal muscle"> skeletal muscle</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound" title=" ultrasound"> ultrasound</a>, <a href="https://publications.waset.org/abstracts/search?q=wearable%20human-machine%20interface" title=" wearable human-machine interface"> wearable human-machine interface</a> </p> <a href="https://publications.waset.org/abstracts/127469/achieving-shear-wave-elastography-by-a-three-element-probe-for-wearable-human-machine-interface" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/127469.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Analysis and Experimental Research on the Influence of Lubricating Oil on the Transmission Efficiency of New Energy Vehicle Gearbox</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chen%20Yong">Chen Yong</a>, <a href="https://publications.waset.org/abstracts/search?q=Bi%20Wangyang"> Bi Wangyang</a>, <a href="https://publications.waset.org/abstracts/search?q=Zang%20Libin"> Zang Libin</a>, <a href="https://publications.waset.org/abstracts/search?q=Li%20Jinkai"> Li Jinkai</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheng%20Xiaowei"> Cheng Xiaowei</a>, <a href="https://publications.waset.org/abstracts/search?q=Liu%20Jinmin"> Liu Jinmin</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Miao"> Yu Miao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> New energy vehicle power transmission systems continue to develop in the direction of high torque, high speed, and high efficiency. The cooling and lubrication of the motor and the transmission system are integrated, and new requirements are placed on the lubricants for the transmission system. The effects of traditional lubricants and special lubricants for new energy vehicles on transmission efficiency were studied through experiments and simulation methods. A mathematical model of the transmission efficiency of the lubricating oil in the gearbox was established. The power loss of each part was analyzed according to the working conditions. The relationship between the speed and the characteristics of different lubricating oil products on the power loss of the stirring oil was discussed. The minimum oil film thickness was required for the life of the gearbox. The accuracy of the calculation results was verified by the transmission efficiency test conducted on the two-motor integrated test bench. The results show that the efficiency increases first and then decreases with the increase of the speed and decreases with the increase of the kinematic viscosity of the lubricant. The increase of the kinematic viscosity amplifies the transmission power loss caused by the high speed. New energy vehicle special lubricants have less attenuation of transmission efficiency in the range above mid-speed. The research results provide a theoretical basis and guidance for the evaluation and selection of transmission efficiency of gearbox lubricants for new energy vehicles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=new%20energy%20vehicles" title="new energy vehicles">new energy vehicles</a>, <a href="https://publications.waset.org/abstracts/search?q=lubricants" title=" lubricants"> lubricants</a>, <a href="https://publications.waset.org/abstracts/search?q=transmission%20efficiency" title=" transmission efficiency"> transmission efficiency</a>, <a href="https://publications.waset.org/abstracts/search?q=kinematic%20viscosity" title=" kinematic viscosity"> kinematic viscosity</a>, <a href="https://publications.waset.org/abstracts/search?q=test%20and%20simulation" title=" test and simulation"> test and simulation</a> </p> <a href="https://publications.waset.org/abstracts/128894/analysis-and-experimental-research-on-the-influence-of-lubricating-oil-on-the-transmission-efficiency-of-new-energy-vehicle-gearbox" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/128894.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">131</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> The Impact of Prior Cancer History on the Prognosis of Salivary Gland Cancer Patients: A Population-based Study from the Surveillance, Epidemiology, and End Results (SEER) Database</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Junhong%20Li">Junhong Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Danni%20Cheng"> Danni Cheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaxin%20Luo"> Yaxin Luo</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Yi"> Xiaowei Yi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ke%20Qiu"> Ke Qiu</a>, <a href="https://publications.waset.org/abstracts/search?q=Wendu%20Pang"> Wendu Pang</a>, <a href="https://publications.waset.org/abstracts/search?q=Minzi%20Mao"> Minzi Mao</a>, <a href="https://publications.waset.org/abstracts/search?q=Yufang%20Rao"> Yufang Rao</a>, <a href="https://publications.waset.org/abstracts/search?q=Yao%20Song"> Yao Song</a>, <a href="https://publications.waset.org/abstracts/search?q=Jianjun%20Ren"> Jianjun Ren</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Zhao"> Yu Zhao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The number of multiple cancer patients was increasing, and the impact of prior cancer history on salivary gland cancer patients remains unclear. Methods: Clinical, demographic and pathological information on salivary gland cancer patients were retrospectively collected from the Surveillance, Epidemiology, and End Results (SEER) database from 2004 to 2017, and the characteristics and prognosis between patients with a prior cancer and those without prior caner were compared. Univariate and multivariate cox proportional regression models were used for the analysis of prognosis. A risk score model was established to exam the impact of treatment on patients with a prior cancer in different risk groups. Results: A total of 9098 salivary gland cancer patients were identified, and 1635 of them had a prior cancer history. Salivary gland cancer patients with prior cancer had worse survival compared with those without a prior cancer (p<0.001). Patients with a different type of first cancer had a distinct prognosis (p<0.001), and longer latent time was associated with better survival (p=0.006) in the univariate model, although both became nonsignificant in the multivariate model. Salivary gland cancer patients with a prior cancer were divided into low-risk (n= 321), intermediate-risk (n=223), and high-risk (n=62) groups and the results showed that patients at high risk could benefit from surgery, radiation therapy, and chemotherapy, and those at intermediate risk could benefit from surgery. Conclusion: Prior cancer history had an adverse impact on the survival of salivary gland cancer patients, and individualized treatment should be seriously considered for them. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=prior%20cancer%20history" title="prior cancer history">prior cancer history</a>, <a href="https://publications.waset.org/abstracts/search?q=prognosis" title=" prognosis"> prognosis</a>, <a href="https://publications.waset.org/abstracts/search?q=salivary%20gland%20cancer" title=" salivary gland cancer"> salivary gland cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=SEER" title=" SEER"> SEER</a> </p> <a href="https://publications.waset.org/abstracts/145306/the-impact-of-prior-cancer-history-on-the-prognosis-of-salivary-gland-cancer-patients-a-population-based-study-from-the-surveillance-epidemiology-and-end-results-seer-database" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145306.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">146</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Offline Parameter Identification and State-of-Charge Estimation for Healthy and Aged Electric Vehicle Batteries Based on the Combined Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Zhang">Xiaowei Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Min%20Xu"> Min Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Saeid%20Habibi"> Saeid Habibi</a>, <a href="https://publications.waset.org/abstracts/search?q=Fengjun%20Yan"> Fengjun Yan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ryan%20Ahmed"> Ryan Ahmed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recently, Electric Vehicles (EVs) have received extensive consideration since they offer a more sustainable and greener transportation alternative compared to fossil-fuel propelled vehicles. Lithium-Ion (Li-ion) batteries are increasingly being deployed in EVs because of their high energy density, high cell-level voltage, and low rate of self-discharge. Since Li-ion batteries represent the most expensive component in the EV powertrain, accurate monitoring and control strategies must be executed to ensure their prolonged lifespan. The Battery Management System (BMS) has to accurately estimate parameters such as the battery State-of-Charge (SOC), State-of-Health (SOH), and Remaining Useful Life (RUL). In order for the BMS to estimate these parameters, an accurate and control-oriented battery model has to work collaboratively with a robust state and parameter estimation strategy. Since battery physical parameters, such as the internal resistance and diffusion coefficient change depending on the battery state-of-life (SOL), the BMS has to be adaptive to accommodate for this change. In this paper, an extensive battery aging study has been conducted over 12-months period on 5.4 Ah, 3.7 V Lithium polymer cells. Instead of using fixed charging/discharging aging cycles at fixed C-rate, a set of real-world driving scenarios have been used to age the cells. The test has been interrupted every 5% capacity degradation by a set of reference performance tests to assess the battery degradation and track model parameters. As battery ages, the combined model parameters are optimized and tracked in an offline mode over the entire batteries lifespan. Based on the optimized model, a state and parameter estimation strategy based on the Extended Kalman Filter (EKF) and the relatively new Smooth Variable Structure Filter (SVSF) have been applied to estimate the SOC at various states of life. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lithium-ion%20batteries" title="lithium-ion batteries">lithium-ion batteries</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20algorithm%20optimization" title=" genetic algorithm optimization"> genetic algorithm optimization</a>, <a href="https://publications.waset.org/abstracts/search?q=battery%20aging%20test" title=" battery aging test"> battery aging test</a>, <a href="https://publications.waset.org/abstracts/search?q=parameter%20identification" title=" parameter identification"> parameter identification</a> </p> <a href="https://publications.waset.org/abstracts/52283/offline-parameter-identification-and-state-of-charge-estimation-for-healthy-and-aged-electric-vehicle-batteries-based-on-the-combined-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/52283.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">267</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> An Aptasensor Based on Magnetic Relaxation Switch and Controlled Magnetic Separation for the Sensitive Detection of Pseudomonas aeruginosa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fei%20Jia">Fei Jia</a>, <a href="https://publications.waset.org/abstracts/search?q=Xingjian%20Bai"> Xingjian Bai</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Zhang"> Xiaowei Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Wenjie%20Yan"> Wenjie Yan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ruitong%20Dai"> Ruitong Dai</a>, <a href="https://publications.waset.org/abstracts/search?q=Xingmin%20Li"> Xingmin Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Jozef%20Kokini"> Jozef Kokini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pseudomonas aeruginosa is a Gram-negative, aerobic, opportunistic human pathogen that is present in the soil, water, and food. This microbe has been recognized as a representative food-borne spoilage bacterium that can lead to many types of infections. Considering the casualties and property loss caused by P. aeruginosa, the development of a rapid and reliable technique for the detection of P. aeruginosa is crucial. The whole-cell aptasensor, an emerging biosensor using aptamer as a capture probe to bind to the whole cell, for food-borne pathogens detection has attracted much attention due to its convenience and high sensitivity. Here, a low-field magnetic resonance imaging (LF-MRI) aptasensor for the rapid detection of P. aeruginosa was developed. The basic detection principle of the magnetic relaxation switch (MRSw) nanosensor lies on the ‘T₂-shortening’ effect of magnetic nanoparticles in NMR measurements. Briefly speaking, the transverse relaxation time (T₂) of neighboring water protons get shortened when magnetic nanoparticles are clustered due to the cross-linking upon the recognition and binding of biological targets, or simply when the concentration of the magnetic nanoparticles increased. Such shortening is related to both the state change (aggregation or dissociation) and the concentration change of magnetic nanoparticles and can be detected using NMR relaxometry or MRI scanners. In this work, two different sizes of magnetic nanoparticles, which are 10 nm (MN₁₀) and 400 nm (MN₄₀₀) in diameter, were first immobilized with anti- P. aeruginosa aptamer through 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) chemistry separately, to capture and enrich the P. aeruginosa cells. When incubating with the target, a ‘sandwich’ (MN₁₀-bacteria-MN₄₀₀) complex are formed driven by the bonding of MN400 with P. aeruginosa through aptamer recognition, as well as the conjugate aggregation of MN₁₀ on the surface of P. aeruginosa. Due to the different magnetic performance of the MN₁₀ and MN₄₀₀ in the magnetic field caused by their different saturation magnetization, the MN₁₀-bacteria-MN₄₀₀ complex, as well as the unreacted MN₄₀₀ in the solution, can be quickly removed by magnetic separation, and as a result, only unreacted MN₁₀ remain in the solution. The remaining MN₁₀, which are superparamagnetic and stable in low field magnetic field, work as a signal readout for T₂ measurement. Under the optimum condition, the LF-MRI platform provides both image analysis and quantitative detection of P. aeruginosa, with the detection limit as low as 100 cfu/mL. The feasibility and specificity of the aptasensor are demonstrated in detecting real food samples and validated by using plate counting methods. Only two steps and less than 2 hours needed for the detection procedure, this robust aptasensor can detect P. aeruginosa with a wide linear range from 3.1 ×10² cfu/mL to 3.1 ×10⁷ cfu/mL, which is superior to conventional plate counting method and other molecular biology testing assay. Moreover, the aptasensor has a potential to detect other bacteria or toxins by changing suitable aptamers. Considering the excellent accuracy, feasibility, and practicality, the whole-cell aptasensor provides a promising platform for a quick, direct and accurate determination of food-borne pathogens at cell-level. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=magnetic%20resonance%20imaging" title="magnetic resonance imaging">magnetic resonance imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=meat%20spoilage" title=" meat spoilage"> meat spoilage</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20aeruginosa" title=" P. aeruginosa"> P. aeruginosa</a>, <a href="https://publications.waset.org/abstracts/search?q=transverse%20relaxation%20time" title=" transverse relaxation time"> transverse relaxation time</a> </p> <a href="https://publications.waset.org/abstracts/99014/an-aptasensor-based-on-magnetic-relaxation-switch-and-controlled-magnetic-separation-for-the-sensitive-detection-of-pseudomonas-aeruginosa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99014.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">152</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th 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