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class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28637444 coauthored" data-work_id="28637444" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28637444/A_New_Multiplexed_Real_Time_PCR_Assay_to_Detect_Campylobacter_jejuni_C_coli_C_lari_and_C_upsaliensis">A New Multiplexed Real-Time PCR Assay to Detect Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28637444" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text 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jejuni","url":"https://www.academia.edu/Documents/in/Campylobacter_jejuni?f_ri=39719"},{"id":1264920,"name":"Mutation Rate","url":"https://www.academia.edu/Documents/in/Mutation_Rate?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16920555" data-work_id="16920555" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16920555/Method_development_and_validation_for_determination_of_thiosultap_sodium_thiocyclam_and_nereistoxin_in_pepper_matrix">Method development and validation for determination of thiosultap sodium, thiocyclam, and nereistoxin in pepper matrix</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This work reports a method for extraction and analysis of thiosultap sodium, thiocyclam, and nereistoxin in pepper. Different extraction methods were tested to attain the best recoveries. The final extraction method combines acetonitrile... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16920555" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This work reports a method for extraction and analysis of thiosultap sodium, thiocyclam, and nereistoxin in pepper. Different extraction methods were tested to attain the best recoveries. The final extraction method combines acetonitrile extraction in an acidic medium with ultrasonic extraction followed by a cleanup step with anhydrous MgSO(4). The analyses were performed on a Linear Ion Trap Quadrupole LC-MS/MS in negative mode for thiosultap sodium and in positive mode for thiocyclam and nereistoxin. Recovery studies carried out on peppers spiked at different fortification levels (20 and 200 μg∙kg(-1)) yielded average recoveries in the range 58-87% with RSD (%) values below 20%. Calibration curves covering two orders of magnitude were performed and they were linear over the concentration range studied (0.001-0.5 mg∙l(-1)). Instrumental detection limits were in the low μg∙kg(-1) range. Stability studies of thiosultap sodium in water were performed by evaluating a 100-μg∙l(-1) solution of this compound in water. It was analyzed over 7 days, after which more than 80% degradation of thiosultap sodium could be observed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16920555" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="50c1dfedb611da5bca777d0065e74a88" rel="nofollow" data-download="{"attachment_id":42374424,"asset_id":16920555,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42374424/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="16715264" href="https://surrey.academia.edu/SubhamoyBhattacharya">Subhamoy Bhattacharya</a><script data-card-contents-for-user="16715264" type="text/json">{"id":16715264,"first_name":"Subhamoy","last_name":"Bhattacharya","domain_name":"surrey","page_name":"SubhamoyBhattacharya","display_name":"Subhamoy Bhattacharya","profile_url":"https://surrey.academia.edu/SubhamoyBhattacharya?f_ri=39719","photo":"https://0.academia-photos.com/16715264/4568320/6122536/s65_subhamoy.bhattacharya.jpg"}</script></span></span></li><li class="js-paper-rank-work_16920555 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16920555"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16920555, container: ".js-paper-rank-work_16920555", }); 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Different extraction methods were tested to attain the best recoveries. The final extraction method combines acetonitrile extraction in an acidic medium with ultrasonic extraction followed by a cleanup step with anhydrous MgSO(4). The analyses were performed on a Linear Ion Trap Quadrupole LC-MS/MS in negative mode for thiosultap sodium and in positive mode for thiocyclam and nereistoxin. Recovery studies carried out on peppers spiked at different fortification levels (20 and 200 μg∙kg(-1)) yielded average recoveries in the range 58-87% with RSD (%) values below 20%. Calibration curves covering two orders of magnitude were performed and they were linear over the concentration range studied (0.001-0.5 mg∙l(-1)). Instrumental detection limits were in the low μg∙kg(-1) range. Stability studies of thiosultap sodium in water were performed by evaluating a 100-μg∙l(-1) solution of this compound in water. 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u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/50498340/Selection_of_aptamers_against_Ara_h_1_protein_for_FO_SPR_biosensing_of_peanut_allergens_in_food_matrices">Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/50498340" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5b2e61cdcd6c81b4ee3de453c5603e08" rel="nofollow" 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aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices","created_at":"2021-08-01T04:48:44.554-07:00","url":"https://www.academia.edu/50498340/Selection_of_aptamers_against_Ara_h_1_protein_for_FO_SPR_biosensing_of_peanut_allergens_in_food_matrices?f_ri=39719","dom_id":"work_50498340","summary":null,"downloadable_attachments":[{"id":68460987,"asset_id":50498340,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":19039811,"first_name":"Dinh Minh","last_name":"Tran","domain_name":"independent","page_name":"DinhMinhTran","display_name":"Dinh Minh Tran","profile_url":"https://independent.academia.edu/DinhMinhTran?f_ri=39719","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=39719","nofollow":false},{"id":1131,"name":"Biomedical 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Analysis","url":"https://www.academia.edu/Documents/in/Equipment_Failure_Analysis?f_ri=39719"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS?f_ri=39719"},{"id":1338314,"name":"Aptamer","url":"https://www.academia.edu/Documents/in/Aptamer?f_ri=39719"},{"id":1412233,"name":"Biosensing Techniques","url":"https://www.academia.edu/Documents/in/Biosensing_Techniques?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_32921894" data-work_id="32921894" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/32921894/Analysis_of_T_2_and_HT2_toxins_in_cereal_grains_by_immunoaffinity_clean_up_and_liquid_chromatography_with_fluorescence_detection">Analysis of T-2 and HT2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A sensitive, precise and accurate method has been developed for the simultaneous determination of T-2 and HT-2 toxins in cereal grains at ppb levels using high-performance liquid chromatography (HPLC) with fluorescence detection and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_32921894" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A sensitive, precise and accurate method has been developed for the simultaneous determination of T-2 and HT-2 toxins in cereal grains at ppb levels using high-performance liquid chromatography (HPLC) with fluorescence detection and 1-antroylnitrile (1-AN) as labeling reagent after immunoaffinity clean-up. Cereal samples were extracted with methanol/water (90:10, v/v), and the extracts were cleaned-up through commercially available immunoaffinity columns containing</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32921894" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1d78d121a6fac7d5d00d2bd6f190d2ee" rel="nofollow" data-download="{"attachment_id":53056555,"asset_id":32921894,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/53056555/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="64069904" href="https://independent.academia.edu/PascaleMichelangelo">Michelangelo Pascale</a><script data-card-contents-for-user="64069904" type="text/json">{"id":64069904,"first_name":"Michelangelo","last_name":"Pascale","domain_name":"independent","page_name":"PascaleMichelangelo","display_name":"Michelangelo Pascale","profile_url":"https://independent.academia.edu/PascaleMichelangelo?f_ri=39719","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_32921894 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="32921894"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 32921894, container: ".js-paper-rank-work_32921894", }); 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$(".js-view-count[data-work-id=32921894]").text(description); $(".js-view-count-work_32921894").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_32921894").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="32921894"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="923" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84384" href="https://www.academia.edu/Documents/in/Maize">Maize</a><script data-card-contents-for-ri="84384" type="text/json">{"id":84384,"name":"Maize","url":"https://www.academia.edu/Documents/in/Maize?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32921894]'), work: {"id":32921894,"title":"Analysis of T-2 and HT2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection","created_at":"2017-05-09T06:23:43.958-07:00","url":"https://www.academia.edu/32921894/Analysis_of_T_2_and_HT2_toxins_in_cereal_grains_by_immunoaffinity_clean_up_and_liquid_chromatography_with_fluorescence_detection?f_ri=39719","dom_id":"work_32921894","summary":"A sensitive, precise and accurate method has been developed for the simultaneous determination of T-2 and HT-2 toxins in cereal grains at ppb levels using high-performance liquid chromatography (HPLC) with fluorescence detection and 1-antroylnitrile (1-AN) as labeling reagent after immunoaffinity clean-up. Cereal samples were extracted with methanol/water (90:10, v/v), and the extracts were cleaned-up through commercially available immunoaffinity columns containing","downloadable_attachments":[{"id":53056555,"asset_id":32921894,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":64069904,"first_name":"Michelangelo","last_name":"Pascale","domain_name":"independent","page_name":"PascaleMichelangelo","display_name":"Michelangelo Pascale","profile_url":"https://independent.academia.edu/PascaleMichelangelo?f_ri=39719","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=39719","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":84384,"name":"Maize","url":"https://www.academia.edu/Documents/in/Maize?f_ri=39719","nofollow":false},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=39719"},{"id":192319,"name":"Cereals","url":"https://www.academia.edu/Documents/in/Cereals?f_ri=39719"},{"id":201241,"name":"Affinity chromatography","url":"https://www.academia.edu/Documents/in/Affinity_chromatography?f_ri=39719"},{"id":225787,"name":"High Performance Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography?f_ri=39719"},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=39719"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=39719"},{"id":398287,"name":"Liquid Chromatography","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography?f_ri=39719"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody?f_ri=39719"},{"id":991311,"name":"Signal to Noise Ratio","url":"https://www.academia.edu/Documents/in/Signal_to_Noise_Ratio?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1492653" data-work_id="1492653" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1492653/Recent_trends_in_biosensors">Recent trends in biosensors</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Rapid advances in biosensors have recently been reported. This has been possible due to rapid growth in the development of new biomaterials such as conducting polymers, copolymers and sol gels etc and the reported improvements in sensing... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1492653" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Rapid advances in biosensors have recently been reported. This has been possible due to rapid growth in the development of new biomaterials such as conducting polymers, copolymers and sol gels etc and the reported improvements in sensing techniques. Biosensors are miniaturized devices employing biochemical molecular recognition as the basis for a selective analysis. The response generated as a result of biochemical reaction is detected by a transducer to give a signal (optical/electrical/thermal) that can be used with or without amplification for the estimation of the concentration of an analyte in a given test sample. Among the various biosensors, electrochemical sensors, especially amperometric biosensors presently hold a leading position.Due to specificity, portability, simplicity, high sensitivity, potential ability for real-time and on-site analysis coupled with the speed and low cost, biosensors have been projected to have applications in food analysis, environment control, clinical detection, drug and agriculture industries etc. Besides this, biosensors offer exciting opportunities for numerous decentralized clinical applications, ranging from emergency room screening, home self testing and alternative site testing, continuous and real-time in vivo monitoring. New generation of biosensors combining new bioreceptors with the ever-growing number of transducers is emerging. The present paper highlights some of the recent advances in the area of biosensors contributed by our laboratory.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1492653" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bf2c0de68820e9a268d724bb08499b2b" rel="nofollow" data-download="{"attachment_id":50949957,"asset_id":1492653,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50949957/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="1402389" href="https://ignou.academia.edu/SandeepChaubey">Sandeep Chaubey</a><script data-card-contents-for-user="1402389" type="text/json">{"id":1402389,"first_name":"Sandeep","last_name":"Chaubey","domain_name":"ignou","page_name":"SandeepChaubey","display_name":"Sandeep Chaubey","profile_url":"https://ignou.academia.edu/SandeepChaubey?f_ri=39719","photo":"https://0.academia-photos.com/1402389/507500/637886/s65_sandeep.chaubey.jpg"}</script></span></span></li><li class="js-paper-rank-work_1492653 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1492653"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1492653, container: ".js-paper-rank-work_1492653", }); 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This has been possible due to rapid growth in the development of new biomaterials such as conducting polymers, copolymers and sol gels etc and the reported improvements in sensing techniques. Biosensors are miniaturized devices employing biochemical molecular recognition as the basis for a selective analysis. The response generated as a result of biochemical reaction is detected by a transducer to give a signal (optical/electrical/thermal) that can be used with or without amplification for the estimation of the concentration of an analyte in a given test sample. Among the various biosensors, electrochemical sensors, especially amperometric biosensors presently hold a leading position.Due to specificity, portability, simplicity, high sensitivity, potential ability for real-time and on-site analysis coupled with the speed and low cost, biosensors have been projected to have applications in food analysis, environment control, clinical detection, drug and agriculture industries etc. Besides this, biosensors offer exciting opportunities for numerous decentralized clinical applications, ranging from emergency room screening, home self testing and alternative site testing, continuous and real-time in vivo monitoring. New generation of biosensors combining new bioreceptors with the ever-growing number of transducers is emerging. The present paper highlights some of the recent advances in the area of biosensors contributed by our laboratory.","downloadable_attachments":[{"id":50949957,"asset_id":1492653,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":1402389,"first_name":"Sandeep","last_name":"Chaubey","domain_name":"ignou","page_name":"SandeepChaubey","display_name":"Sandeep Chaubey","profile_url":"https://ignou.academia.edu/SandeepChaubey?f_ri=39719","photo":"https://0.academia-photos.com/1402389/507500/637886/s65_sandeep.chaubey.jpg"}],"research_interests":[{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":49834,"name":"Molecular Recognition","url":"https://www.academia.edu/Documents/in/Molecular_Recognition?f_ri=39719","nofollow":false},{"id":76049,"name":"Emergency Room","url":"https://www.academia.edu/Documents/in/Emergency_Room?f_ri=39719","nofollow":false},{"id":80414,"name":"Mathematical Sciences","url":"https://www.academia.edu/Documents/in/Mathematical_Sciences?f_ri=39719"},{"id":85458,"name":"Conducting Polymer","url":"https://www.academia.edu/Documents/in/Conducting_Polymer?f_ri=39719"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=39719"},{"id":229390,"name":"Real Time","url":"https://www.academia.edu/Documents/in/Real_Time?f_ri=39719"},{"id":390049,"name":"Electrical Conductivity","url":"https://www.academia.edu/Documents/in/Electrical_Conductivity?f_ri=39719"},{"id":962638,"name":"High Sensitivity","url":"https://www.academia.edu/Documents/in/High_Sensitivity?f_ri=39719"},{"id":1202042,"name":"Electric Conductivity","url":"https://www.academia.edu/Documents/in/Electric_Conductivity?f_ri=39719"},{"id":1275886,"name":"Clinical Application","url":"https://www.academia.edu/Documents/in/Clinical_Application?f_ri=39719"},{"id":2425997,"name":"Site testing","url":"https://www.academia.edu/Documents/in/Site_testing?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3616454" data-work_id="3616454" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3616454/Validation_and_uncertainty_analysis_of_a_multi_residue_method_for_pesticides_in_grapes_using_ethyl_acetate_extraction_and_liquid_chromatography_tandem_mass_spectrometry">Validation and uncertainty analysis of a multi-residue method for pesticides in grapes using ethyl acetate extraction and liquid chromatography–tandem mass spectrometry</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A method was validated for the multi-residue analysis of 82 pesticides in grapes at ≤25 ng/g level. Berry samples (10 g) mixed with sodium sulphate (10 g) were extracted with ethyl acetate (10 mL); cleaned by dispersive solid phase... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3616454" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A method was validated for the multi-residue analysis of 82 pesticides in grapes at ≤25 ng/g level. Berry samples (10 g) mixed with sodium sulphate (10 g) were extracted with ethyl acetate (10 mL); cleaned by dispersive solid phase extraction and the results were obtained by liquid chromatography–tandem mass spectrometry. Reduction in sample size and proportion of ethyl acetate for extraction did not affect accuracy or precision of analysis when compared to the reported methods and was also statistically similar to the QuEChERS technique. The method was rugged (HorRat < 0.5) with <20% measurement uncertainties. Limit of quantification was <10 ng/g with recoveries 70–120% for most pesticides. The method offers cheaper and safer alternative to typical multi-residue analysis methods for grape.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3616454" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1cb9c4bbfc09edf2eb009db5eb07c636" rel="nofollow" data-download="{"attachment_id":50197960,"asset_id":3616454,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50197960/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4341735" href="https://independent.academia.edu/shubhangipatil">shubhangi patil</a><script data-card-contents-for-user="4341735" type="text/json">{"id":4341735,"first_name":"shubhangi","last_name":"patil","domain_name":"independent","page_name":"shubhangipatil","display_name":"shubhangi patil","profile_url":"https://independent.academia.edu/shubhangipatil?f_ri=39719","photo":"https://0.academia-photos.com/4341735/125915176/115285525/s65_shubhangi.patil.jpeg"}</script></span></span></li><li class="js-paper-rank-work_3616454 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3616454"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3616454, container: ".js-paper-rank-work_3616454", }); 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Berry samples (10 g) mixed with sodium sulphate (10 g) were extracted with ethyl acetate (10 mL); cleaned by dispersive solid phase extraction and the results were obtained by liquid chromatography–tandem mass spectrometry. Reduction in sample size and proportion of ethyl acetate for extraction did not affect accuracy or precision of analysis when compared to the reported methods and was also statistically similar to the QuEChERS technique. The method was rugged (HorRat \u003c 0.5) with \u003c20% measurement uncertainties. Limit of quantification was \u003c10 ng/g with recoveries 70–120% for most pesticides. The method offers cheaper and safer alternative to typical multi-residue analysis methods for grape.","downloadable_attachments":[{"id":50197960,"asset_id":3616454,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4341735,"first_name":"shubhangi","last_name":"patil","domain_name":"independent","page_name":"shubhangipatil","display_name":"shubhangi patil","profile_url":"https://independent.academia.edu/shubhangipatil?f_ri=39719","photo":"https://0.academia-photos.com/4341735/125915176/115285525/s65_shubhangi.patil.jpeg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=39719","nofollow":false},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=39719","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=39719","nofollow":false},{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=39719","nofollow":false},{"id":10347,"name":"European Union","url":"https://www.academia.edu/Documents/in/European_Union?f_ri=39719"},{"id":22843,"name":"Pesticide Residues","url":"https://www.academia.edu/Documents/in/Pesticide_Residues?f_ri=39719"},{"id":32910,"name":"Sample Preparation","url":"https://www.academia.edu/Documents/in/Sample_Preparation?f_ri=39719"},{"id":37434,"name":"Quantitative analysis","url":"https://www.academia.edu/Documents/in/Quantitative_analysis?f_ri=39719"},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719"},{"id":43465,"name":"Electrospray","url":"https://www.academia.edu/Documents/in/Electrospray?f_ri=39719"},{"id":61603,"name":"Uncertainty","url":"https://www.academia.edu/Documents/in/Uncertainty?f_ri=39719"},{"id":65590,"name":"Pesticide","url":"https://www.academia.edu/Documents/in/Pesticide?f_ri=39719"},{"id":82563,"name":"Tea","url":"https://www.academia.edu/Documents/in/Tea?f_ri=39719"},{"id":83426,"name":"Solid Phase Extraction","url":"https://www.academia.edu/Documents/in/Solid_Phase_Extraction?f_ri=39719"},{"id":85437,"name":"Pesticides","url":"https://www.academia.edu/Documents/in/Pesticides?f_ri=39719"},{"id":96893,"name":"Calibration","url":"https://www.academia.edu/Documents/in/Calibration?f_ri=39719"},{"id":163275,"name":"Tandem Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Tandem_Mass_Spectrometry?f_ri=39719"},{"id":168891,"name":"Chemical 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Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=39719"},{"id":529560,"name":"Gas Chromatography/mass Spectrometry","url":"https://www.academia.edu/Documents/in/Gas_Chromatography_mass_Spectrometry?f_ri=39719"},{"id":549280,"name":"Reproducibility of Results","url":"https://www.academia.edu/Documents/in/Reproducibility_of_Results?f_ri=39719"},{"id":570860,"name":"Food Contamination","url":"https://www.academia.edu/Documents/in/Food_Contamination?f_ri=39719"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=39719"},{"id":611814,"name":"Correlation coefficient","url":"https://www.academia.edu/Documents/in/Correlation_coefficient?f_ri=39719"},{"id":641216,"name":"Quantitative Analysis","url":"https://www.academia.edu/Documents/in/Quantitative_Analysis-1?f_ri=39719"},{"id":651380,"name":"Vitis","url":"https://www.academia.edu/Documents/in/Vitis?f_ri=39719"},{"id":788711,"name":"Residue","url":"https://www.academia.edu/Documents/in/Residue?f_ri=39719"},{"id":806478,"name":"Method Validation","url":"https://www.academia.edu/Documents/in/Method_Validation?f_ri=39719"},{"id":1647408,"name":"Measurement Uncertainty","url":"https://www.academia.edu/Documents/in/Measurement_Uncertainty?f_ri=39719"},{"id":1745595,"name":"Solvents","url":"https://www.academia.edu/Documents/in/Solvents?f_ri=39719"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9279309" data-work_id="9279309" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9279309/Mineral_Profile_and_Variability_in_Vegetable_Amaranth_Amaranthus_tricolor">Mineral Profile and Variability in Vegetable Amaranth ( Amaranthus tricolor</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Populations in North India depend on a number of vegetable crops of which Amaranthus spp. is the most important since it is the only crop available in the hot summer months when no other foliage crop grows in the field. However, reports... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9279309" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Populations in North India depend on a number of vegetable crops of which Amaranthus spp. is the most important since it is the only crop available in the hot summer months when no other foliage crop grows in the field. However, reports on mineral composition of leaves are rare with absolutely no information on the qualitative improvement of foliage yield with special reference to minerals. Studies on correlation among the minerals as well as with yield and leaf attributes are also lacking. Hence, we report the proximate mineral composition in 30 strains of A. tricolor along with some suggestions for qualitative improvement of the foliage yield with reference to minerals. Our study showed that vegetable amaranth is a rich source of minerals like calcium (1.7±0.04 g/100 g), iron (1233.8±50.02 mg/kg), and zinc (791.7±28.98 mg/kg). The heritability estimates were high for most of the traits, with potassium and calcium showing high values, while comparatively lower values were recorded for magnesium and nickel. Nickel was the only mineral that showed positive correlation with all the minerals, as well as with leaf size and foliage yield. Zinc showed strong positive relationship with iron (0.66**) and manganese (0.74**), and was the only mineral exhibiting significant positive association with foliage yield. This study would be of use in enhancement of selected minerals in different regions according to local preferences and nutrient deficiency prevalent among the populations.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9279309" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fcff5cd51a3954e4c1d78b1301a69f23" rel="nofollow" data-download="{"attachment_id":47833185,"asset_id":9279309,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47833185/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="21358482" href="https://tech4more.academia.edu/SudhirShukla">Sudhir Shukla</a><script data-card-contents-for-user="21358482" type="text/json">{"id":21358482,"first_name":"Sudhir","last_name":"Shukla","domain_name":"tech4more","page_name":"SudhirShukla","display_name":"Sudhir Shukla","profile_url":"https://tech4more.academia.edu/SudhirShukla?f_ri=39719","photo":"https://0.academia-photos.com/21358482/5888682/6687122/s65_sudhir.shukla.jpg"}</script></span></span></li><li class="js-paper-rank-work_9279309 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9279309"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9279309, container: ".js-paper-rank-work_9279309", }); 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$(".js-view-count[data-work-id=9279309]").text(description); $(".js-view-count-work_9279309").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_9279309").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="9279309"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="156" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9533" href="https://www.academia.edu/Documents/in/Minerals">Minerals</a>, <script data-card-contents-for-ri="9533" type="text/json">{"id":9533,"name":"Minerals","url":"https://www.academia.edu/Documents/in/Minerals?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a><script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=9279309]'), work: {"id":9279309,"title":"Mineral Profile and Variability in Vegetable Amaranth ( Amaranthus tricolor","created_at":"2014-11-12T18:03:17.426-08:00","url":"https://www.academia.edu/9279309/Mineral_Profile_and_Variability_in_Vegetable_Amaranth_Amaranthus_tricolor?f_ri=39719","dom_id":"work_9279309","summary":"Populations in North India depend on a number of vegetable crops of which Amaranthus spp. is the most important since it is the only crop available in the hot summer months when no other foliage crop grows in the field. However, reports on mineral composition of leaves are rare with absolutely no information on the qualitative improvement of foliage yield with special reference to minerals. Studies on correlation among the minerals as well as with yield and leaf attributes are also lacking. Hence, we report the proximate mineral composition in 30 strains of A. tricolor along with some suggestions for qualitative improvement of the foliage yield with reference to minerals. Our study showed that vegetable amaranth is a rich source of minerals like calcium (1.7±0.04 g/100 g), iron (1233.8±50.02 mg/kg), and zinc (791.7±28.98 mg/kg). The heritability estimates were high for most of the traits, with potassium and calcium showing high values, while comparatively lower values were recorded for magnesium and nickel. Nickel was the only mineral that showed positive correlation with all the minerals, as well as with leaf size and foliage yield. Zinc showed strong positive relationship with iron (0.66**) and manganese (0.74**), and was the only mineral exhibiting significant positive association with foliage yield. This study would be of use in enhancement of selected minerals in different regions according to local preferences and nutrient deficiency prevalent among the populations.","downloadable_attachments":[{"id":47833185,"asset_id":9279309,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":21358482,"first_name":"Sudhir","last_name":"Shukla","domain_name":"tech4more","page_name":"SudhirShukla","display_name":"Sudhir Shukla","profile_url":"https://tech4more.academia.edu/SudhirShukla?f_ri=39719","photo":"https://0.academia-photos.com/21358482/5888682/6687122/s65_sudhir.shukla.jpg"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=39719","nofollow":false},{"id":9533,"name":"Minerals","url":"https://www.academia.edu/Documents/in/Minerals?f_ri=39719","nofollow":false},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India?f_ri=39719"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=39719"},{"id":70902,"name":"Magnesium","url":"https://www.academia.edu/Documents/in/Magnesium?f_ri=39719"},{"id":158165,"name":"Zinc","url":"https://www.academia.edu/Documents/in/Zinc?f_ri=39719"},{"id":158597,"name":"Iron","url":"https://www.academia.edu/Documents/in/Iron?f_ri=39719"},{"id":160656,"name":"Potassium","url":"https://www.academia.edu/Documents/in/Potassium?f_ri=39719"},{"id":194828,"name":"Nickel","url":"https://www.academia.edu/Documents/in/Nickel?f_ri=39719"},{"id":299285,"name":"Mineral Composition","url":"https://www.academia.edu/Documents/in/Mineral_Composition?f_ri=39719"},{"id":369262,"name":"Amaranthus","url":"https://www.academia.edu/Documents/in/Amaranthus?f_ri=39719"},{"id":649451,"name":"Seasons","url":"https://www.academia.edu/Documents/in/Seasons?f_ri=39719"},{"id":1149996,"name":"Leaf Size","url":"https://www.academia.edu/Documents/in/Leaf_Size?f_ri=39719"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10608976" data-work_id="10608976" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10608976/Heating_and_Sterilization_Technology_for_Long_duration_Space_Missions">Heating and Sterilization Technology for Long-duration Space Missions</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10608976" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="49ec02cdc81b4ab3b1250528d810e857" rel="nofollow" data-download="{"attachment_id":47264735,"asset_id":10608976,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47264735/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="25921817" href="https://independent.academia.edu/PanditRam">Ram Pandit</a><script data-card-contents-for-user="25921817" type="text/json">{"id":25921817,"first_name":"Ram","last_name":"Pandit","domain_name":"independent","page_name":"PanditRam","display_name":"Ram Pandit","profile_url":"https://independent.academia.edu/PanditRam?f_ri=39719","photo":"https://0.academia-photos.com/25921817/11225155/12524830/s65_ram.pandit.jpg"}</script></span></span></li><li class="js-paper-rank-work_10608976 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10608976"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10608976, container: ".js-paper-rank-work_10608976", }); });</script></li><li class="js-percentile-work_10608976 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 10608976; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_10608976"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_10608976 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="10608976"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 10608976; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=10608976]").text(description); $(".js-view-count-work_10608976").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_10608976").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="10608976"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="33661" href="https://www.academia.edu/Documents/in/Heat_and_Mass_Transfer">Heat and Mass Transfer</a>, <script data-card-contents-for-ri="33661" type="text/json">{"id":33661,"name":"Heat and Mass Transfer","url":"https://www.academia.edu/Documents/in/Heat_and_Mass_Transfer?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="48456" href="https://www.academia.edu/Documents/in/Ohmic_Heating">Ohmic Heating</a><script data-card-contents-for-ri="48456" type="text/json">{"id":48456,"name":"Ohmic Heating","url":"https://www.academia.edu/Documents/in/Ohmic_Heating?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=10608976]'), work: {"id":10608976,"title":"Heating and Sterilization Technology for Long-duration Space 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Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":48456,"name":"Ohmic Heating","url":"https://www.academia.edu/Documents/in/Ohmic_Heating?f_ri=39719","nofollow":false},{"id":69542,"name":"Computer Simulation","url":"https://www.academia.edu/Documents/in/Computer_Simulation?f_ri=39719"},{"id":177876,"name":"Sterilization","url":"https://www.academia.edu/Documents/in/Sterilization?f_ri=39719"},{"id":255058,"name":"Metals","url":"https://www.academia.edu/Documents/in/Metals?f_ri=39719"},{"id":283222,"name":"Package","url":"https://www.academia.edu/Documents/in/Package?f_ri=39719"},{"id":527921,"name":"Joule heating","url":"https://www.academia.edu/Documents/in/Joule_heating?f_ri=39719"},{"id":542983,"name":"Heating","url":"https://www.academia.edu/Documents/in/Heating?f_ri=39719"},{"id":846015,"name":"Electrodes","url":"https://www.academia.edu/Documents/in/Electrodes?f_ri=39719"},{"id":911616,"name":"Space Missions","url":"https://www.academia.edu/Documents/in/Space_Missions?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13669272" data-work_id="13669272" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13669272/Exercise_and_associated_dietary_extremes_impact_on_gut_microbial_diversity">Exercise and associated dietary extremes impact on gut microbial diversity</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The commensal microbiota, host immunity and metabolism participate in a signalling network, with diet influencing each component of this triad. In addition to diet, many elements of a modern lifestyle influence the gut microbiota but the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13669272" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The commensal microbiota, host immunity and metabolism participate in a signalling network, with diet influencing each component of this triad. In addition to diet, many elements of a modern lifestyle influence the gut microbiota but the degree to which exercise affects this population is unclear. Therefore, we explored exercise and diet for their impact on the gut microbiota. Since extremes of exercise often accompany extremes of diet, we addressed the issue by studying professional athletes from an international rugby union squad. Two groups were included to control for physical size, age and gender. Compositional analysis of the microbiota was explored by 16S rRNA amplicon sequencing. Each participant completed a detailed food frequency questionnaire. As expected, athletes and controls differed significantly with respect to plasma creatine kinase (a marker of extreme exercise), and inflammatory and metabolic markers. More importantly, athletes had a higher diversity of gut micro-...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13669272" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fa45a1e2c1a03c9e225270730621a386" rel="nofollow" data-download="{"attachment_id":45081825,"asset_id":13669272,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45081825/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32816737" href="https://weillcornell.academia.edu/EamonnQuigley">Eamonn Quigley</a><script data-card-contents-for-user="32816737" type="text/json">{"id":32816737,"first_name":"Eamonn","last_name":"Quigley","domain_name":"weillcornell","page_name":"EamonnQuigley","display_name":"Eamonn Quigley","profile_url":"https://weillcornell.academia.edu/EamonnQuigley?f_ri=39719","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13669272 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13669272"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13669272, container: ".js-paper-rank-work_13669272", }); 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In addition to diet, many elements of a modern lifestyle influence the gut microbiota but the degree to which exercise affects this population is unclear. Therefore, we explored exercise and diet for their impact on the gut microbiota. Since extremes of exercise often accompany extremes of diet, we addressed the issue by studying professional athletes from an international rugby union squad. Two groups were included to control for physical size, age and gender. Compositional analysis of the microbiota was explored by 16S rRNA amplicon sequencing. Each participant completed a detailed food frequency questionnaire. As expected, athletes and controls differed significantly with respect to plasma creatine kinase (a marker of extreme exercise), and inflammatory and metabolic markers. 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u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="923" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="67661" href="https://www.academia.edu/Documents/in/Sewage_sludge">Sewage sludge</a><script data-card-contents-for-ri="67661" type="text/json">{"id":67661,"name":"Sewage sludge","url":"https://www.academia.edu/Documents/in/Sewage_sludge?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=15634067]'), work: {"id":15634067,"title":"Pressurized liquid extraction in the analysis of food and biological 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the 10 European countries participating in the EPIC study</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20746026" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="28e1846ee91a104b7ae767db43281f4e" rel="nofollow" data-download="{"attachment_id":41538904,"asset_id":20746026,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" 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type="text/json">{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="10408" href="https://www.academia.edu/Documents/in/Support_Vector_Machines">Support Vector Machines</a>, <script data-card-contents-for-ri="10408" type="text/json">{"id":10408,"name":"Support Vector Machines","url":"https://www.academia.edu/Documents/in/Support_Vector_Machines?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16137" href="https://www.academia.edu/Documents/in/Food_Chemistry">Food Chemistry</a><script data-card-contents-for-ri="16137" type="text/json">{"id":16137,"name":"Food Chemistry","url":"https://www.academia.edu/Documents/in/Food_Chemistry?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17922351]'), work: 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The metals in the initial aqueous solution were complexed with... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_71365571" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A new, simple and versatile cloud-point extraction (CPE) methodology has been developed for the separation and preconcentration of copper and nickel. 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href="https://www.academia.edu/17867469/Polymerase_chain_reaction_for_the_rapid_identification_of_Clostridium_botulinum_type_A_strains_and_detection_in_food_samples">Polymerase chain reaction for the rapid identification of Clostridium botulinum type A strains and detection in food samples</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A polymerase chain reaction (PCR) was developed for the detection of Clostridium botulinum type A, a cause of human botulism. A two primer set and an oligonucleotide detection probe were used to specifically detect Cl. botulinum type A... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_17867469" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A polymerase chain reaction (PCR) was developed for the detection of Clostridium botulinum type A, a cause of human botulism. A two primer set and an oligonucleotide detection probe were used to specifically detect Cl. botulinum type A neurotoxin gene (BoNT/A). After 40 cycles of amplification, detection of a 798 bp amplified DNA fragment was carried out by agarose gel electrophoresis and Southern blot hybridization. This assay was able to detect 12.5 fg of purified target DNA or five bacteria per reaction. The sensitivity in artificially contaminated food samples after an 18 h enrichment step ranges from 10 to 10(3) bacteria per g according to the type of food samples. No cross-reactions were observed with the other Cl. botulinum toxinotypes and other bacteria found routinely in food. This PCR method may provide a suitable and rapid alternative to standard techniques for detection of Cl. botulinum type A in food samples.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17867469" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9ccd2badca612ac7bcc19af0fd8bb489" rel="nofollow" data-download="{"attachment_id":42221648,"asset_id":17867469,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42221648/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37779205" href="https://independent.academia.edu/PFach">P. 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A two primer set and an oligonucleotide detection probe were used to specifically detect Cl. botulinum type A neurotoxin gene (BoNT/A). After 40 cycles of amplification, detection of a 798 bp amplified DNA fragment was carried out by agarose gel electrophoresis and Southern blot hybridization. This assay was able to detect 12.5 fg of purified target DNA or five bacteria per reaction. The sensitivity in artificially contaminated food samples after an 18 h enrichment step ranges from 10 to 10(3) bacteria per g according to the type of food samples. No cross-reactions were observed with the other Cl. botulinum toxinotypes and other bacteria found routinely in food. This PCR method may provide a suitable and rapid alternative to standard techniques for detection of Cl. botulinum type A in food samples.","downloadable_attachments":[{"id":42221648,"asset_id":17867469,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37779205,"first_name":"P.","last_name":"Fach","domain_name":"independent","page_name":"PFach","display_name":"P. 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type="text/json">{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16062" href="https://www.academia.edu/Documents/in/Starch">Starch</a>, <script data-card-contents-for-ri="16062" type="text/json">{"id":16062,"name":"Starch","url":"https://www.academia.edu/Documents/in/Starch?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16137" href="https://www.academia.edu/Documents/in/Food_Chemistry">Food Chemistry</a><script data-card-contents-for-ri="16137" type="text/json">{"id":16137,"name":"Food Chemistry","url":"https://www.academia.edu/Documents/in/Food_Chemistry?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=47592977]'), work: {"id":47592977,"title":"Nutritional and 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Aldrin, cis-chlordane,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5906276" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In this study, 24 organochlorine pesticide residues in 109 different honey samples collected from stores and open markets in Konya, Turkey were analyzed by gas chromatography-electron capture detection. Aldrin, cis-chlordane, trans-chlordane, oxy-chlordane, 2,4′ -DDE, and 4,4′ -DDE were found in all honey samples. The mean value was 0.0540 μg g − 1 for oxy-chlordane. In the 55 samples of 109, levels of organochlorine pesticide residues of oxy-chlordane were determined as higher than those of Turkish Alimentarius Codex maximum residual limits (MRLs). Other organochlorine pesticide residues also exceeded MRLs except for cis-heptachlor epoxide and α-hexachlorocyclohexane. Since all of the honey samples are found contaminated and most of these samples exceeded MRLs, a control of organochlorine pesticide residues in honey is necessary for consumer health.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5906276" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="965834a9c3bd7180696b048776a718fe" rel="nofollow" data-download="{"attachment_id":36584491,"asset_id":5906276,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/36584491/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="6942299" href="https://aksaray.academia.edu/YavuzSelim%C3%87akmak">Yavuz Selim Çakmak</a><script data-card-contents-for-user="6942299" type="text/json">{"id":6942299,"first_name":"Yavuz Selim","last_name":"Çakmak","domain_name":"aksaray","page_name":"YavuzSelimÇakmak","display_name":"Yavuz Selim Çakmak","profile_url":"https://aksaray.academia.edu/YavuzSelim%C3%87akmak?f_ri=39719","photo":"https://0.academia-photos.com/6942299/4900385/32115798/s65_yavuz_selim._akmak.jpg"}</script></span></span></li><li class="js-paper-rank-work_5906276 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5906276"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5906276, container: ".js-paper-rank-work_5906276", }); 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$(".js-view-count[data-work-id=5906276]").text(description); $(".js-view-count-work_5906276").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_5906276").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="5906276"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="10966" href="https://www.academia.edu/Documents/in/Turkey">Turkey</a>, <script data-card-contents-for-ri="10966" type="text/json">{"id":10966,"name":"Turkey","url":"https://www.academia.edu/Documents/in/Turkey?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22843" href="https://www.academia.edu/Documents/in/Pesticide_Residues">Pesticide Residues</a>, <script data-card-contents-for-ri="22843" type="text/json">{"id":22843,"name":"Pesticide Residues","url":"https://www.academia.edu/Documents/in/Pesticide_Residues?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a><script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=5906276]'), work: {"id":5906276,"title":"Determination of some organochlorine pesticide residues in honeys from Konya, Turkey","created_at":"2014-01-31T19:49:06.292-08:00","url":"https://www.academia.edu/5906276/Determination_of_some_organochlorine_pesticide_residues_in_honeys_from_Konya_Turkey?f_ri=39719","dom_id":"work_5906276","summary":"In this study, 24 organochlorine pesticide residues in 109 different honey samples collected from stores and open markets in Konya, Turkey were analyzed by gas chromatography-electron capture detection. Aldrin, cis-chlordane, trans-chlordane, oxy-chlordane, 2,4′ -DDE, and 4,4′ -DDE were found in all honey samples. The mean value was 0.0540 μg g − 1 for oxy-chlordane. In the 55 samples of 109, levels of organochlorine pesticide residues of oxy-chlordane were determined as higher than those of Turkish Alimentarius Codex maximum residual limits (MRLs). Other organochlorine pesticide residues also exceeded MRLs except for cis-heptachlor epoxide and α-hexachlorocyclohexane. Since all of the honey samples are found contaminated and most of these samples exceeded MRLs, a control of organochlorine pesticide residues in honey is necessary for consumer health.","downloadable_attachments":[{"id":36584491,"asset_id":5906276,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":6942299,"first_name":"Yavuz Selim","last_name":"Çakmak","domain_name":"aksaray","page_name":"YavuzSelimÇakmak","display_name":"Yavuz Selim Çakmak","profile_url":"https://aksaray.academia.edu/YavuzSelim%C3%87akmak?f_ri=39719","photo":"https://0.academia-photos.com/6942299/4900385/32115798/s65_yavuz_selim._akmak.jpg"}],"research_interests":[{"id":10966,"name":"Turkey","url":"https://www.academia.edu/Documents/in/Turkey?f_ri=39719","nofollow":false},{"id":22843,"name":"Pesticide Residues","url":"https://www.academia.edu/Documents/in/Pesticide_Residues?f_ri=39719","nofollow":false},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":64758,"name":"Honey","url":"https://www.academia.edu/Documents/in/Honey?f_ri=39719"},{"id":166448,"name":"Gas Chromatography","url":"https://www.academia.edu/Documents/in/Gas_Chromatography?f_ri=39719"},{"id":282637,"name":"Environmental monitoring and assessment","url":"https://www.academia.edu/Documents/in/Environmental_monitoring_and_assessment?f_ri=39719"},{"id":1133132,"name":"Environmental","url":"https://www.academia.edu/Documents/in/Environmental?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card 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tomatoes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Two sensitive, selective, and precise methods for the determination of pyriproxyfen and pyridalyl insecticide residues in tomatoes have been developed. The first method is HPLC with UV detection in which pyriproxyfen and pyridalyl were... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_71835025" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Two sensitive, selective, and precise methods for the determination of pyriproxyfen and pyridalyl insecticide residues in tomatoes have been developed. The first method is HPLC with UV detection in which pyriproxyfen and pyridalyl were extracted with ethyl acetate and acetone, respectively, followed by cleanup using column chromatography. The recoveries ranged from 86.03 to 94.55 for pyriproxyfen and 95.08 to 99.38% for pyridalyl in tomato samples. The LOD of the method was 0.217 ppm for pyriproxyfen and 0.1866 ppm for pyridalyl. The second method depends on direct fluorometric determination of pyriproxyfen and pyridalyl in acetic and sulfuric acid at excitation and emission wavelengths of 320 and 646 nm, respectively. The recoveries of pyriproxyfen and pyridalyl in tomato samples ranged from 88 to 98% and 86 to 93%, respectively. The LOD of the method was 0.146 ppm for pyriproxyfen and 0.078 ppm for pyridalyl. Both methods were applied successfully to determine residues and rate of...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/71835025" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f7750548e51f1a61bd2ef2f2841bb0df" rel="nofollow" data-download="{"attachment_id":81019711,"asset_id":71835025,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/81019711/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="40592898" href="https://shams.academia.edu/NohaFathyElAzab">Noha Fathy El Azab</a><script data-card-contents-for-user="40592898" type="text/json">{"id":40592898,"first_name":"Noha","last_name":"Fathy El Azab","domain_name":"shams","page_name":"NohaFathyElAzab","display_name":"Noha Fathy El Azab","profile_url":"https://shams.academia.edu/NohaFathyElAzab?f_ri=39719","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_71835025 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="71835025"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 71835025, container: ".js-paper-rank-work_71835025", }); 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The first method is HPLC with UV detection in which pyriproxyfen and pyridalyl were extracted with ethyl acetate and acetone, respectively, followed by cleanup using column chromatography. The recoveries ranged from 86.03 to 94.55 for pyriproxyfen and 95.08 to 99.38% for pyridalyl in tomato samples. The LOD of the method was 0.217 ppm for pyriproxyfen and 0.1866 ppm for pyridalyl. The second method depends on direct fluorometric determination of pyriproxyfen and pyridalyl in acetic and sulfuric acid at excitation and emission wavelengths of 320 and 646 nm, respectively. The recoveries of pyriproxyfen and pyridalyl in tomato samples ranged from 88 to 98% and 86 to 93%, respectively. The LOD of the method was 0.146 ppm for pyriproxyfen and 0.078 ppm for pyridalyl. Both methods were applied successfully to determine residues and rate of...","downloadable_attachments":[{"id":81019711,"asset_id":71835025,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":40592898,"first_name":"Noha","last_name":"Fathy El Azab","domain_name":"shams","page_name":"NohaFathyElAzab","display_name":"Noha Fathy El Azab","profile_url":"https://shams.academia.edu/NohaFathyElAzab?f_ri=39719","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=39719","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=39719","nofollow":false},{"id":22843,"name":"Pesticide Residues","url":"https://www.academia.edu/Documents/in/Pesticide_Residues?f_ri=39719","nofollow":false},{"id":39719,"name":"Food 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href="https://www.academia.edu/17795200/Microbiological_hazard_identification_and_exposure_assessment_of_food_prepared_and_served_in_rural_households_of_Lungwena_Malawi">Microbiological hazard identification and exposure assessment of food prepared and served in rural households of Lungwena, Malawi</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17795200" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bb8b7a3361b1cbe7473afecfd82fe008" rel="nofollow" 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u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="14907" href="https://www.academia.edu/Documents/in/Industrial_Biotechnology">Industrial Biotechnology</a>, <script data-card-contents-for-ri="14907" type="text/json">{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16664" href="https://www.academia.edu/Documents/in/Risk_assessment">Risk assessment</a>, <script data-card-contents-for-ri="16664" type="text/json">{"id":16664,"name":"Risk assessment","url":"https://www.academia.edu/Documents/in/Risk_assessment?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="31632" href="https://www.academia.edu/Documents/in/Food_Microbiology">Food Microbiology</a><script data-card-contents-for-ri="31632" type="text/json">{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17795200]'), work: {"id":17795200,"title":"Microbiological hazard identification and exposure assessment of food prepared and served in rural households of Lungwena, 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class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 55577045, container: ".js-paper-rank-work_55577045", }); });</script></li><li class="js-percentile-work_55577045 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 55577045; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_55577045"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_55577045 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="55577045"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 55577045; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=55577045]").text(description); $(".js-view-count-work_55577045").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_55577045").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="55577045"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="9478" href="https://www.academia.edu/Documents/in/Diet">Diet</a>, <script data-card-contents-for-ri="9478" type="text/json">{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22053" href="https://www.academia.edu/Documents/in/Carotenoids">Carotenoids</a>, <script data-card-contents-for-ri="22053" type="text/json">{"id":22053,"name":"Carotenoids","url":"https://www.academia.edu/Documents/in/Carotenoids?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a><script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=55577045]'), work: {"id":55577045,"title":"The development and application of a carotenoid database for fruits, vegetables, and selected multicomponent foods","created_at":"2021-10-05T03:33:44.925-07:00","url":"https://www.academia.edu/55577045/The_development_and_application_of_a_carotenoid_database_for_fruits_vegetables_and_selected_multicomponent_foods?f_ri=39719","dom_id":"work_55577045","summary":null,"downloadable_attachments":[{"id":71384488,"asset_id":55577045,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":13660889,"first_name":"Elaine","last_name":"Lanza","domain_name":"cancer","page_name":"ElaineLanza","display_name":"Elaine Lanza","profile_url":"https://cancer.academia.edu/ElaineLanza?f_ri=39719","photo":"https://0.academia-photos.com/13660889/4520387/5233674/s65_elaine.lanza.jpg_oh_9cf20f0e8fce9eb3636a3c49dbbdfd5d_oe_548b6c51___gda___1419531704_99aac2634163147b15af9ea224b4a56a"}],"research_interests":[{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=39719","nofollow":false},{"id":22053,"name":"Carotenoids","url":"https://www.academia.edu/Documents/in/Carotenoids?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=39719","nofollow":false},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=39719"},{"id":160814,"name":"Fruit","url":"https://www.academia.edu/Documents/in/Fruit?f_ri=39719"},{"id":208544,"name":"Vegetables","url":"https://www.academia.edu/Documents/in/Vegetables?f_ri=39719"},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged?f_ri=39719"},{"id":375257,"name":"Beta Carotene","url":"https://www.academia.edu/Documents/in/Beta_Carotene?f_ri=39719"},{"id":375260,"name":"Lutein","url":"https://www.academia.edu/Documents/in/Lutein?f_ri=39719"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult?f_ri=39719"},{"id":1863718,"name":"The American","url":"https://www.academia.edu/Documents/in/The_American?f_ri=39719"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_52737448" data-work_id="52737448" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/52737448/Portion_Sizes_in_Dietary_Assessment_Issues_and_Policy_Implications">Portion Sizes in Dietary Assessment: Issues and Policy Implications</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm 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class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32510545" href="https://independent.academia.edu/MNestle">Marion Nestle</a><script data-card-contents-for-user="32510545" type="text/json">{"id":32510545,"first_name":"Marion","last_name":"Nestle","domain_name":"independent","page_name":"MNestle","display_name":"Marion Nestle","profile_url":"https://independent.academia.edu/MNestle?f_ri=39719","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_52737448 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="52737448"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 52737448, container: 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window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=52737448]").text(description); $(".js-view-count-work_52737448").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_52737448").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="52737448"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="7428" href="https://www.academia.edu/Documents/in/Health_Education">Health Education</a>, <script data-card-contents-for-ri="7428" type="text/json">{"id":7428,"name":"Health Education","url":"https://www.academia.edu/Documents/in/Health_Education?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="85636" href="https://www.academia.edu/Documents/in/Food_Labeling">Food Labeling</a><script data-card-contents-for-ri="85636" type="text/json">{"id":85636,"name":"Food 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Education","url":"https://www.academia.edu/Documents/in/Health_Education?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=39719","nofollow":false},{"id":85636,"name":"Food Labeling","url":"https://www.academia.edu/Documents/in/Food_Labeling?f_ri=39719","nofollow":false},{"id":98134,"name":"United States","url":"https://www.academia.edu/Documents/in/United_States?f_ri=39719"},{"id":218820,"name":"Eating","url":"https://www.academia.edu/Documents/in/Eating?f_ri=39719"},{"id":324399,"name":"Dietary Assessment","url":"https://www.academia.edu/Documents/in/Dietary_Assessment?f_ri=39719"},{"id":327850,"name":"Questionnaires","url":"https://www.academia.edu/Documents/in/Questionnaires?f_ri=39719"},{"id":369093,"name":"Food habits","url":"https://www.academia.edu/Documents/in/Food_habits?f_ri=39719"},{"id":584436,"name":"United States Department of Agriculture","url":"https://www.academia.edu/Documents/in/United_States_Department_of_Agriculture?f_ri=39719"},{"id":1236470,"name":"Nutrition Policy","url":"https://www.academia.edu/Documents/in/Nutrition_Policy?f_ri=39719"},{"id":2474760,"name":"United States Food and Drug Administration","url":"https://www.academia.edu/Documents/in/United_States_Food_and_Drug_Administration?f_ri=39719"},{"id":2637271,"name":"Reference standards","url":"https://www.academia.edu/Documents/in/Reference_standards?f_ri=39719"},{"id":2922956,"name":"Psychology and Cognitive Sciences","url":"https://www.academia.edu/Documents/in/Psychology_and_Cognitive_Sciences?f_ri=39719"},{"id":3225903,"name":"Nutrition assessment","url":"https://www.academia.edu/Documents/in/Nutrition_assessment?f_ri=39719"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16992557" data-work_id="16992557" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16992557/Detection_of_genetically_modified_DNA_sequences_in_milk_from_The_Italian_market">Detection of genetically modified DNA sequences in milk from The Italian market</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The possible transfer and accumulation of novel DNA and/or proteins in food for human consumption derived from animals receiving genetically modified (GM) feed is at present the object of scientific dispute. A number of studies failed to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16992557" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The possible transfer and accumulation of novel DNA and/or proteins in food for human consumption derived from animals receiving genetically modified (GM) feed is at present the object of scientific dispute. A number of studies failed to identify GM DNA in milk, meat, or eggs derived from livestock receiving GM feed ingredients. The present study was performed in order to: (i) develop a valid protocol by PCR and multicomponent analysis for the detection of specific DNA sequences in milk, focused on GM maize and GM soybean; (ii) assess the stability of transgenic DNA after pasteurization treatment and (iii) determine the presence of GM DNA sequences in milk samples collected from the Italian market. Results from the screening of 60 samples of 12 different milk brands demonstrated the presence of GM maize sequences in 15 (25%) and of GM soybean sequences in 7 samples (11.7%). Our screening methodology shows a very high sensitivity and the use of an automatic identification of the amplified products increases its specificity and reliability. Moreover, we demonstrated that the pasteurization process is not able to degrade the DNA sequences in spiked milk samples. The detection of GM DNA in milk can be interpreted as an indicator of fecal or airborne contamination, respectively, with feed DNA or feed particles, although an alternative source of contamination, possibly recognizable in the natural environment can be suggested. Further studies, performed on a larger number of milk samples, are needed to understand the likely source of contamination of milk collected from the Italian market.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16992557" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="559bae740ceee06fb5b59c6d1f7f180c" rel="nofollow" data-download="{"attachment_id":42363364,"asset_id":16992557,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42363364/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="36517063" href="https://unict.academia.edu/AntonellaAgodi">Antonella Agodi</a><script data-card-contents-for-user="36517063" type="text/json">{"id":36517063,"first_name":"Antonella","last_name":"Agodi","domain_name":"unict","page_name":"AntonellaAgodi","display_name":"Antonella Agodi","profile_url":"https://unict.academia.edu/AntonellaAgodi?f_ri=39719","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_16992557 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16992557"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16992557, container: ".js-paper-rank-work_16992557", }); });</script></li><li class="js-percentile-work_16992557 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16992557; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16992557"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16992557 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16992557"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16992557; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16992557]").text(description); $(".js-view-count-work_16992557").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16992557").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16992557"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="41012" href="https://www.academia.edu/Documents/in/Recombinant_DNA_Technology">Recombinant DNA Technology</a>, <script data-card-contents-for-ri="41012" type="text/json">{"id":41012,"name":"Recombinant DNA Technology","url":"https://www.academia.edu/Documents/in/Recombinant_DNA_Technology?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="45213" href="https://www.academia.edu/Documents/in/Italy">Italy</a>, <script data-card-contents-for-ri="45213" type="text/json">{"id":45213,"name":"Italy","url":"https://www.academia.edu/Documents/in/Italy?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84384" href="https://www.academia.edu/Documents/in/Maize">Maize</a><script data-card-contents-for-ri="84384" type="text/json">{"id":84384,"name":"Maize","url":"https://www.academia.edu/Documents/in/Maize?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16992557]'), work: {"id":16992557,"title":"Detection of genetically modified DNA sequences in milk from The Italian market","created_at":"2015-10-19T05:50:18.230-07:00","url":"https://www.academia.edu/16992557/Detection_of_genetically_modified_DNA_sequences_in_milk_from_The_Italian_market?f_ri=39719","dom_id":"work_16992557","summary":"The possible transfer and accumulation of novel DNA and/or proteins in food for human consumption derived from animals receiving genetically modified (GM) feed is at present the object of scientific dispute. A number of studies failed to identify GM DNA in milk, meat, or eggs derived from livestock receiving GM feed ingredients. The present study was performed in order to: (i) develop a valid protocol by PCR and multicomponent analysis for the detection of specific DNA sequences in milk, focused on GM maize and GM soybean; (ii) assess the stability of transgenic DNA after pasteurization treatment and (iii) determine the presence of GM DNA sequences in milk samples collected from the Italian market. Results from the screening of 60 samples of 12 different milk brands demonstrated the presence of GM maize sequences in 15 (25%) and of GM soybean sequences in 7 samples (11.7%). Our screening methodology shows a very high sensitivity and the use of an automatic identification of the amplified products increases its specificity and reliability. Moreover, we demonstrated that the pasteurization process is not able to degrade the DNA sequences in spiked milk samples. The detection of GM DNA in milk can be interpreted as an indicator of fecal or airborne contamination, respectively, with feed DNA or feed particles, although an alternative source of contamination, possibly recognizable in the natural environment can be suggested. Further studies, performed on a larger number of milk samples, are needed to understand the likely source of contamination of milk collected from the Italian market.","downloadable_attachments":[{"id":42363364,"asset_id":16992557,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36517063,"first_name":"Antonella","last_name":"Agodi","domain_name":"unict","page_name":"AntonellaAgodi","display_name":"Antonella Agodi","profile_url":"https://unict.academia.edu/AntonellaAgodi?f_ri=39719","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":41012,"name":"Recombinant DNA Technology","url":"https://www.academia.edu/Documents/in/Recombinant_DNA_Technology?f_ri=39719","nofollow":false},{"id":45213,"name":"Italy","url":"https://www.academia.edu/Documents/in/Italy?f_ri=39719","nofollow":false},{"id":84384,"name":"Maize","url":"https://www.academia.edu/Documents/in/Maize?f_ri=39719","nofollow":false},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=39719"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=39719"},{"id":129386,"name":"DNA sequence design","url":"https://www.academia.edu/Documents/in/DNA_sequence_design?f_ri=39719"},{"id":177876,"name":"Sterilization","url":"https://www.academia.edu/Documents/in/Sterilization?f_ri=39719"},{"id":188229,"name":"Soybean","url":"https://www.academia.edu/Documents/in/Soybean?f_ri=39719"},{"id":238368,"name":"Milk","url":"https://www.academia.edu/Documents/in/Milk?f_ri=39719"},{"id":323164,"name":"Genetically Modified","url":"https://www.academia.edu/Documents/in/Genetically_Modified?f_ri=39719"},{"id":340277,"name":"Natural Environment","url":"https://www.academia.edu/Documents/in/Natural_Environment?f_ri=39719"},{"id":354934,"name":"Soybeans","url":"https://www.academia.edu/Documents/in/Soybeans?f_ri=39719"},{"id":370863,"name":"Zea mays","url":"https://www.academia.edu/Documents/in/Zea_mays?f_ri=39719"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=39719"},{"id":962638,"name":"High Sensitivity","url":"https://www.academia.edu/Documents/in/High_Sensitivity?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_29198718 coauthored" data-work_id="29198718" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/29198718/Comparison_of_the_dietary_cobalt_intake_in_three_different_Australian_diets">Comparison of the dietary cobalt intake in three different Australian diets</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Differences in the dietary intake of cobalt were assessed for vegans, lacto-ovo-vegetarian and non-vegetarian Australians using food intake logs, and daily or average trend recall over three months. A significant decrease in cobalt intake... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_29198718" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Differences in the dietary intake of cobalt were assessed for vegans, lacto-ovo-vegetarian and non-vegetarian Australians using food intake logs, and daily or average trend recall over three months. A significant decrease in cobalt intake was observed for the lacto-ovo-vegetarian population compared with the intake in vegans and omnivores. There is no RDI for cobalt, however, the cobalt intake of Australians was similar to that reported in other countries. Microflora above the terminal ileum have been shown to produce significant amounts of biologically available vitamin B12. This study was unable to demonstrate a correlation between elemental cobalt intake and serum vitamin B12 concentrations in humans, as has been shown in vitro.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/29198718" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="955b41721a1e626c1edf1c74a319c43e" rel="nofollow" data-download="{"attachment_id":49647275,"asset_id":29198718,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49647275/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="55113041" href="https://independent.academia.edu/BevanHokin">Bevan Hokin</a><script data-card-contents-for-user="55113041" type="text/json">{"id":55113041,"first_name":"Bevan","last_name":"Hokin","domain_name":"independent","page_name":"BevanHokin","display_name":"Bevan Hokin","profile_url":"https://independent.academia.edu/BevanHokin?f_ri=39719","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-29198718">+1</span><div class="hidden js-additional-users-29198718"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/JohnAshton3">John Ashton</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-29198718'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-29198718').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_29198718 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="29198718"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 29198718, container: ".js-paper-rank-work_29198718", }); });</script></li><li class="js-percentile-work_29198718 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 29198718; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_29198718"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_29198718 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="29198718"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 29198718; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=29198718]").text(description); $(".js-view-count-work_29198718").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_29198718").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="29198718"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="591" href="https://www.academia.edu/Documents/in/Nutrition_and_Dietetics">Nutrition and Dietetics</a>, <script data-card-contents-for-ri="591" type="text/json">{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6697" href="https://www.academia.edu/Documents/in/Australia">Australia</a>, <script data-card-contents-for-ri="6697" type="text/json">{"id":6697,"name":"Australia","url":"https://www.academia.edu/Documents/in/Australia?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9478" href="https://www.academia.edu/Documents/in/Diet">Diet</a>, <script data-card-contents-for-ri="9478" type="text/json">{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=39719","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a><script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=29198718]'), work: {"id":29198718,"title":"Comparison of the dietary cobalt intake in three different Australian diets","created_at":"2016-10-16T14:52:24.824-07:00","url":"https://www.academia.edu/29198718/Comparison_of_the_dietary_cobalt_intake_in_three_different_Australian_diets?f_ri=39719","dom_id":"work_29198718","summary":"Differences in the dietary intake of cobalt were assessed for vegans, lacto-ovo-vegetarian and non-vegetarian Australians using food intake logs, and daily or average trend recall over three months. A significant decrease in cobalt intake was observed for the lacto-ovo-vegetarian population compared with the intake in vegans and omnivores. There is no RDI for cobalt, however, the cobalt intake of Australians was similar to that reported in other countries. Microflora above the terminal ileum have been shown to produce significant amounts of biologically available vitamin B12. This study was unable to demonstrate a correlation between elemental cobalt intake and serum vitamin B12 concentrations in humans, as has been shown in vitro.","downloadable_attachments":[{"id":49647275,"asset_id":29198718,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":55113041,"first_name":"Bevan","last_name":"Hokin","domain_name":"independent","page_name":"BevanHokin","display_name":"Bevan Hokin","profile_url":"https://independent.academia.edu/BevanHokin?f_ri=39719","photo":"/images/s65_no_pic.png"},{"id":55311722,"first_name":"John","last_name":"Ashton","domain_name":"independent","page_name":"JohnAshton3","display_name":"John Ashton","profile_url":"https://independent.academia.edu/JohnAshton3?f_ri=39719","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=39719","nofollow":false},{"id":6697,"name":"Australia","url":"https://www.academia.edu/Documents/in/Australia?f_ri=39719","nofollow":false},{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=39719","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=39719","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=39719"},{"id":92239,"name":"Asia Pacific","url":"https://www.academia.edu/Documents/in/Asia_Pacific?f_ri=39719"},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=39719"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=39719"},{"id":168454,"name":"Cobalt","url":"https://www.academia.edu/Documents/in/Cobalt?f_ri=39719"},{"id":241044,"name":"Food intake","url":"https://www.academia.edu/Documents/in/Food_intake?f_ri=39719"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult?f_ri=39719"},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=39719"},{"id":1716295,"name":"vitamin B deficiency","url":"https://www.academia.edu/Documents/in/vitamin_B_deficiency?f_ri=39719"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27458456" data-work_id="27458456" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27458456/Analysis_of_volatile_compounds_in_five_jackfruit_Artocarpus_heterophyllus_L_cultivars_using_solid_phase_microextraction_SPME_and_gas_chromatography_time_of_flight_mass_spectrometry_GC_TOFMS_">Analysis of volatile compounds in five jackfruit (Artocarpus heterophyllus L.) cultivars using solid-phase microextraction (SPME) and gas chromatography-time-of-flight mass spectrometry (GC-TOFMS)</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27458456" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1aac6051b421a91294f5ac41ebad30f7" rel="nofollow" data-download="{"attachment_id":47717681,"asset_id":27458456,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47717681/download_file?st=MTczMjM5NDAxOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5526355" href="https://independent.academia.edu/DzulkiflyHashim">Dzulkifly Hashim</a><script 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