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solid lipid nanoparticle</title> <meta name="description" content="Search results for: catanionic solid lipid nanoparticle"> <meta name="keywords" content="catanionic solid lipid nanoparticle"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research 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method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="catanionic solid lipid nanoparticle"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 3237</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: catanionic solid lipid nanoparticle</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3237</span> Effect of Wheat Germ Agglutinin- and Lactoferrin-Grafted Catanionic Solid Lipid Nanoparticles on Targeting Delivery of Etoposide to Glioblastoma Multiforme</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yung-Chih%20Kuo">Yung-Chih Kuo</a>, <a href="https://publications.waset.org/abstracts/search?q=I-Hsin%20Wang"> I-Hsin Wang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Catanionic solid lipid nanoparticles (CASLNs) with surface wheat germ agglutinin (WGA) and lactoferrin (Lf) were formulated for entrapping and releasing etoposide (ETP), crossing the blood–brain barrier (BBB), and inhibiting the growth of glioblastoma multiforme (GBM). Microemulsified ETP-CASLNs were modified with WGA and Lf for permeating a cultured monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes and for treating malignant U87MG cells. Experimental evidence revealed that an increase in the concentration of catanionic surfactant from 5 μM to 7.5 μM reduced the particle size. When the concentration of catanionic surfactant increased from 7.5 μM to 12.5 μM, the particle size increased, yielding a minimal diameter of WGA-Lf-ETP-CASLNs at 7.5 μM of catanionic surfactant. An increase in the weight percentage of BW from 25% to 75% enlarged WGA-Lf-ETP-CASLNs. In addition, an increase in the concentration of catanionic surfactant from 5 to 15 μM increased the absolute value of zeta potential of WGA-Lf-ETP-CASLNs. It was intriguing that the increment of the charge as a function of the concentration of catanionic surfactant was approximately linear. WGA-Lf-ETP-CASLNs revealed an integral structure with smooth particle contour, displayed a lighter exterior layer of catanionic surfactant, WGA, and Lf and showed a rigid interior region of solid lipids. A variation in the concentration of catanionic surfactant between 5 μM and 15 μM yielded a maximal encapsulation efficiency of ETP ata 7.5 μM of catanionic surfactant. An increase in the concentration of Lf/WGA decreased the grafting efficiency of Lf/WGA. Also, an increase in the weight percentage of ETP decreased its encapsulation efficiency. Moreover, the release rate of ETP from WGA-Lf-ETP-CASLNs reduced with increasing concentration of catanionic surfactant, and WGA-Lf-ETP-CASLNs at 12.5 μM of catanionic surfactant exhibited a feature of sustained release. The order in the viability of HBMECs was ETP-CASLNs ≅ Lf-ETP-CASLNs ≅ WGA-Lf-ETP-CASLNs > ETP. The variation in the transendothelial electrical resistance (TEER) and permeability of propidium iodide (PI) was negligible when the concentration of Lf increased. Furthermore, an increase in the concentration of WGA from 0.2 to 0.6 mg/mL insignificantly altered the TEER and permeability of PI. When the concentration of Lf increased from 2.5 to 7.5 μg/mL and the concentration of WGA increased from 2.5 to 5 μg/mL, the enhancement in the permeability of ETP was minor. However, 10 μg/mL of Lf promoted the permeability of ETP using Lf-ETP-CASLNs, and 5 and 10 μg/mL of WGA could considerably improve the permeability of ETP using WGA-Lf-ETP-CASLNs. The order in the efficacy of inhibiting U87MG cells was WGA-Lf-ETP-CASLNs > Lf-ETP-CASLNs > ETP-CASLNs > ETP. As a result, WGA-Lf-ETP-CASLNs reduced the TEER, enhanced the permeability of PI, induced a minor cytotoxicity to HBMECs, increased the permeability of ETP across the BBB, and improved the antiproliferative efficacy of U87MG cells. The grafting of WGA and Lf is crucial to control the medicinal property of ETP-CASLNs and WGA-Lf-ETP-CASLNs can be promising colloidal carriers in GBM management. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=catanionic%20solid%20lipid%20nanoparticle" title="catanionic solid lipid nanoparticle">catanionic solid lipid nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=etoposide" title=" etoposide"> etoposide</a>, <a href="https://publications.waset.org/abstracts/search?q=glioblastoma%20multiforme" title=" glioblastoma multiforme"> glioblastoma multiforme</a>, <a href="https://publications.waset.org/abstracts/search?q=lactoferrin" title=" lactoferrin"> lactoferrin</a>, <a href="https://publications.waset.org/abstracts/search?q=wheat%20germ%20agglutinin" title=" wheat germ agglutinin"> wheat germ agglutinin</a> </p> <a href="https://publications.waset.org/abstracts/69376/effect-of-wheat-germ-agglutinin-and-lactoferrin-grafted-catanionic-solid-lipid-nanoparticles-on-targeting-delivery-of-etoposide-to-glioblastoma-multiforme" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69376.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">237</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3236</span> Cationic Solid Lipid Nanoparticles Conjugated with Anti-Melantransferrin and Apolipoprotein E for Delivering Doxorubicin to U87MG Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yung-Chih%20Kuo">Yung-Chih Kuo</a>, <a href="https://publications.waset.org/abstracts/search?q=Yung-I%20Lou"> Yung-I Lou</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cationic solid lipid nanoparticles (CSLNs) with anti-melanotransferrin (AMT) and apolipoprotein E (ApoE) were used to carry antimitotic doxorubicin (Dox) across the blood–brain barrier (BBB) for glioblastoma multiforme (GBM) treatment. Dox-loaded CSLNs were prepared in microemulsion, grafted covalently with AMT and ApoE, and applied to human brain microvascular endothelial cells (HBMECs), human astrocytes, and U87MG cells. Experimental results revealed that an increase in the weight percentage of stearyl amine (SA) from 0% to 20% increased the size of AMT-ApoE-Dox-CSLNs. In addition, an increase in the stirring rate from 150 rpm to 450 rpm decreased the size of AMT-ApoE-Dox-CSLNs. An increase in the weight percentage of SA from 0% to 20% enhanced the zeta potential of AMT-ApoE-Dox-CSLNs. Moreover, an increase in the stirring rate from 150 rpm to 450 rpm reduced the zeta potential of AMT-ApoE-Dox-CSLNs. AMT-ApoE-Dox-CSLNs exhibited a spheroid-like geometry, a minor irregular boundary deviating from spheroid, and a somewhat distorted surface with a few zigzags and sharp angles. The encapsulation efficiency of Dox in CSLNs decreased with increasing weight percentage of Dox and the order in the encapsulation efficiency of Dox was 10% SA > 20% SA > 0% SA. However, the reverse order was true for the release rate of Dox, suggesting that AMT-ApoE-Dox-CSLNs containing 10% SA had better-sustained release characteristics. An increase in the concentration of AMT from 2.5 to 7.5 μg/mL slightly decreased the grafting efficiency of AMT and an increase in that from 7.5 to 10 μg/mL significantly decreased the grafting efficiency. Furthermore, an increase in the concentration of ApoE from 2.5 to 5 μg/mL slightly reduced the grafting efficiency of ApoE and an increase in that from 5 to 10 μg/mL significantly reduced the grafting efficiency. Also, AMT-ApoE-Dox-CSLNs at 10 μg/mL of ApoE could slightly reduce the transendothelial electrical resistance (TEER) and increase the permeability of propidium iodide (PI). An incorporation of 10 μg/mL of ApoE could reduce the TEER and increase the permeability of PI. AMT-ApoE-Dox-CSLNs at 10 μg/mL of AMT and 5-10 μg/mL of ApoE could significantly enhance the permeability of Dox across the BBB. AMT-ApoE-Dox-CSLNs did not induce serious cytotoxicity to HBMECs. The viability of HBMECs was in the following order: AMT-ApoE-Dox-CSLNs = AMT-Dox-CSLNs = Dox-CSLNs > Dox. The order in the efficacy of inhibiting U87MG cells was AMT-ApoE-Dox-CSLNs > AMT-Dox-CSLNs > Dox-CSLNs > Dox. A surface modification of AMT and ApoE could promote the delivery of AMT-ApoE-Dox-CSLNs to cross the BBB via melanotransferrin and low density lipoprotein receptor. Thus, AMT-ApoE-Dox-CSLNs have appropriate physicochemical properties and can be a potential colloidal delivery system for brain tumor chemotherapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti-melanotransferrin" title="anti-melanotransferrin">anti-melanotransferrin</a>, <a href="https://publications.waset.org/abstracts/search?q=apolipoprotein%20E" title=" apolipoprotein E"> apolipoprotein E</a>, <a href="https://publications.waset.org/abstracts/search?q=cationic%20catanionic%20solid%20lipid%20nanoparticle" title=" cationic catanionic solid lipid nanoparticle"> cationic catanionic solid lipid nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=doxorubicin" title=" doxorubicin"> doxorubicin</a>, <a href="https://publications.waset.org/abstracts/search?q=U87MG%20cells" title=" U87MG cells "> U87MG cells </a> </p> <a href="https://publications.waset.org/abstracts/69377/cationic-solid-lipid-nanoparticles-conjugated-with-anti-melantransferrin-and-apolipoprotein-e-for-delivering-doxorubicin-to-u87mg-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69377.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">284</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3235</span> Topical Delivery of Griseofulvin via Lipid Nanoparticles</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yann%20Jean%20Tan">Yann Jean Tan</a>, <a href="https://publications.waset.org/abstracts/search?q=Hui%20Meng%20Er"> Hui Meng Er</a>, <a href="https://publications.waset.org/abstracts/search?q=Choy%20Sin%20Lee"> Choy Sin Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Shew%20Fung%20Wong"> Shew Fung Wong</a>, <a href="https://publications.waset.org/abstracts/search?q=Wen%20Huei%20Lim"> Wen Huei Lim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Griseofulvin is a long standing fungistatic agent against dermatophytosis. Nevertheless, it has several drawbacks such as poor and highly variable bio availability, long duration of treatment, systemic side effects and drug interactions. Targeted treatment for the superficial skin infection, dermatophytosis via topical route could be beneficial. Nevertheless, griseofulvin is only available in the form of oral preparation. Hence, it generates interest in developing a topical formulation for griseofulvin, by using lipid nano particle as the vehicle. Lipid nanoparticle is a submicron colloidal carrier with a core that is solid in nature (lipid). It has combined advantages of various traditional carriers and is a promising vehicle for topical delivery. The griseofulvin loaded lipid nano particles produced using high pressure homogenization method were characterized and investigated for its skin targeting effect in vitro. It has a mean particle size of 179.8±4.9 nm with polydispersity index of 0.306±0.011. Besides, it showed higher skin permeation and better skin targeting effect compared to the griseofulvin suspension. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lipid%20nanoparticles" title="lipid nanoparticles">lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=griseofulvin" title=" griseofulvin"> griseofulvin</a>, <a href="https://publications.waset.org/abstracts/search?q=topical" title=" topical"> topical</a>, <a href="https://publications.waset.org/abstracts/search?q=dermatophytosis" title=" dermatophytosis"> dermatophytosis</a> </p> <a href="https://publications.waset.org/abstracts/18028/topical-delivery-of-griseofulvin-via-lipid-nanoparticles" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18028.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">458</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3234</span> Lipid Nanoparticles for Spironolactone Delivery: Physicochemical Characteristics, Stability and Invitro Release</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20R.%20Kelidari">H. R. Kelidari</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Saeedi"> M. Saeedi</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Akbari"> J. Akbari</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Morteza-Semnani"> K. Morteza-Semnani</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Valizadeh"> H. Valizadeh </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Spironolactoe (SP) a synthetic steroid diuretic is a poorly water-soluble drug with a low and variable oral bioavailability. Regarding to the good solubility of SP in lipid materials, SP loaded Solid lipid nanoparticles (SP-SLNs) and nanostructured lipid carrier (SP-SLNs) were thus prepared in this work for accelerating dissolution of this drug. The SP loaded NLC with stearic acid (SA) as solid lipid and different Oleic Acid (OA) as liquid lipid content and SLN without OA were prepared by probe ultrasonication method. With increasing the percentage of OA from 0 to 30 wt% in SLN/NLC, the average size and zeta potential of nanoparticles felled down and entrapment efficiency (EE %) rose dramatically. The obtained micrograph particles showed pronounced spherical shape. Differential Scanning Calorimeter (DSC) measurements indicated that the presence of OA reduced the melting temperature and melting enthalpy of solid lipid in NLC structure. The results reflected good long-term stability of the nanoparticles and the measurements show that the particle size remains lower in NLC compare to SLN formulations, 6 months after production. Dissolution of SP-SLN and SP-NLC was about 5.1 and 7.2 times faster than raw drugs in 120 min respectively. These results indicated that the SP loaded NLC containing 70:30 solid lipid to liquid lipid ratio is a suitable carrier of SP with improved drug EE and steady drug release properties. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20release" title="drug release">drug release</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20nanoparticles" title=" lipid nanoparticles"> lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=spironolactone" title=" spironolactone"> spironolactone</a>, <a href="https://publications.waset.org/abstracts/search?q=stability" title=" stability"> stability</a> </p> <a href="https://publications.waset.org/abstracts/30285/lipid-nanoparticles-for-spironolactone-delivery-physicochemical-characteristics-stability-and-invitro-release" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30285.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">331</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3233</span> Delivery of Doxorubicin to Glioblastoma Multiforme Using Solid Lipid Nanoparticles with Surface Aprotinin and Melanotransferrin Antibody for Enhanced Chemotherapy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yung-Chih%20Kuo">Yung-Chih Kuo</a>, <a href="https://publications.waset.org/abstracts/search?q=I-Hsuan%20Lee"> I-Hsuan Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Solid lipid nanoparticles (SLNs) conjugated with aprotinin (Apr) and melanotransferrin antibody (Anti-MTf) were used to carry doxorubicin (Dox) across the blood–brain barrier (BBB) for glioblastoma multiforme (GBM) chemotherapy. Dox-entrapped SLNs with grafted Apr and Anti-MTf (Apr-Anti-MTf-Dox-SLNs) were applied to a cultured monolayer comprising human brain-microvascular endothelial cells (HBMECs) with regulation of human astrocyte (HAs) and to a proliferated colony of U87MG cells. Based on the average particle diameter, zeta potential, entrapping efficiency of Dox, and grafting efficiency of Apr and Anti-MTf, we found that 40% (w/w) 1,2-dipalmitoyl-sn-glycero-3-phosphocholine in lipids were appropriate for fabricating Apr-Anti-MTf-Dox-SLNs. In addition, Apr-Anti-MTf-Dox-SLNs could prevent Dox from fast dissolution and did not induce a serious cytotoxicity to HBMECs and HAs when compared with free Dox. Moreover, the treatments with Apr-Anti-MTf-Dox-SLNs enhanced the ability of Dox to infuse the BBB and to inhibit the growth of GBM. The current Apr-Anti-MTf-Dox-SLNs can be a promising pharmacotherapeutic preparation to penetrate the BBB for malignant brain tumor treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticle" title="solid lipid nanoparticle">solid lipid nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=glioblastoma%20multiforme" title=" glioblastoma multiforme"> glioblastoma multiforme</a>, <a href="https://publications.waset.org/abstracts/search?q=blood%E2%80%93brain%20barrier" title=" blood–brain barrier"> blood–brain barrier</a>, <a href="https://publications.waset.org/abstracts/search?q=doxorubicin" title=" doxorubicin"> doxorubicin</a> </p> <a href="https://publications.waset.org/abstracts/38612/delivery-of-doxorubicin-to-glioblastoma-multiforme-using-solid-lipid-nanoparticles-with-surface-aprotinin-and-melanotransferrin-antibody-for-enhanced-chemotherapy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38612.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">361</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3232</span> Preparation and Characterization of Diclofenac Sodium Loaded Solid Lipid Nanoparticle</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Oktavia%20Eka%20Puspita">Oktavia Eka Puspita</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The possibility of using Solid Lipid Nanoparticles (SLN) for topical use is an interesting feature concerning this system has occlusive properties on the skin surface therefore enhance the penetration of drugs through the stratum corneum by increased hydration. This advantage can be used to enhance the drug penetration of topical delivery such as Diclofenac sodium for the relief of signs and symptoms of osteoarthritis, rheumatoid arthritis and ankylosing spondylitis. The purpose of this study was focused on the preparation and physical characterization of Diclofenac sodium loaded SLN (D-SLN). D loaded SLN were prepared by hot homogenization followed by ultrasonication technique. Since the occlusion factor of SLN is related to its particle size the formulation of D-SLN in present study two formulations different in its surfactant contents were prepared to investigate the difference of the particle size resulted. Surfactants selected for preparation of formulation A (FA) were lecithin soya and Tween 80 whereas formulation B (FB) were lecithin soya, Tween 80, and Sodium Lauryl Sulphate. D-SLN were characterized for particle size and distribution, polydispersity index (PI), zeta potential using Beckman-Coulter Delsa™ Nano. Overall, the particle size obtained from FA was larger than FB. FA has 90% of the particles were above 1000 nm, while FB has 90% were below 100 nm. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticles" title="solid lipid nanoparticles">solid lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=hot%20homogenization%20technique" title=" hot homogenization technique"> hot homogenization technique</a>, <a href="https://publications.waset.org/abstracts/search?q=particle%20size%20analysis" title=" particle size analysis"> particle size analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=topical%20administration" title=" topical administration"> topical administration</a> </p> <a href="https://publications.waset.org/abstracts/16904/preparation-and-characterization-of-diclofenac-sodium-loaded-solid-lipid-nanoparticle" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16904.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">500</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3231</span> Famotidine Loaded Solid Lipid Nanoparticles (SLN) for Oral Delivery System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rachmat%20Mauludin">Rachmat Mauludin</a>, <a href="https://publications.waset.org/abstracts/search?q=Novita%20R.%20Kusuma"> Novita R. Kusuma</a>, <a href="https://publications.waset.org/abstracts/search?q=Diky%20Mudhakir"> Diky Mudhakir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Famotidine (FMT) is one of used substances in the treatment of hiperacidity and peptic ulcer, administered orally and parenterally via intravenous injection. Oral administration, which is more favorable, has been reported to have many obstacles in the process of the treatment, includes decreasing the bioavailability of FMT. This research was aimed to prepare FMT in form of solid lipid nanoparticles (SLN) with size ranging between 100-200 nm. The research was carried out also by optimizing factors that may affect physical stability of SLN. Formulation of Famotidine SLN was carried out by optimizing factors, such as duration of homogenization and sonication, lipid concentration, stabilizer composition and stabilizer concentration. SLN physical stability was evaluated (particle size distribution) for 42 days in 3 diferent temperatures. Entrapment efficiency and drug loading was determined indirectly and directly. The morphology of SLN was visualized by transmission electron microscope (TEM). In vitro release study of FMT was conducted in 2 mediums, at pH of 1.2 and 7.4. Chemical stability of FMT was determined by quantifying the concentration of FMT within 42 days. Famotidin SLN consisted of GMS as lipid and poloxamer 188, lecithin, and polysorbate 80 as stabilizers. Homogenization and sonication was performed for 5 minutes and 10 minutes. Physyical stability of nanoparticles at 3 different temperatures was no significant difference. The best formula was physically stable until 42 days with mean particle size below 200 nm. Nanoparticles produced was able to entrap FMT until 86.6%. Evaluation by TEM showed that nanoparticles was spherical and solid. In medium pH of 1.2, FMT was released only 30% during 4 hour. On the other hand, within 4 hours SLN could release FMT completely in medium pH of 7.4. The FMT concentration in nanoparticles dispersion was maintained until 95% in 42 days (40oC, RH 75%). Famotidine SLN was able to be produced with mean particle size ranging between 100-200 nm and physically stable for 42 days. SLN could be loaded by 86,6% of FMT. Morphologically, obtained SLN was spheric and solid. During 4 hours in medium pH of 1.2 and 7.4, FMT was released until 30% and 100%, respectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticle%20%28SLN%29" title="solid lipid nanoparticle (SLN)">solid lipid nanoparticle (SLN)</a>, <a href="https://publications.waset.org/abstracts/search?q=famotidine%20%28FMT%29" title=" famotidine (FMT)"> famotidine (FMT)</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical%20properties" title=" physicochemical properties"> physicochemical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=release%20study" title=" release study"> release study</a> </p> <a href="https://publications.waset.org/abstracts/19816/famotidine-loaded-solid-lipid-nanoparticles-sln-for-oral-delivery-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19816.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">360</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3230</span> Novel Solid Lipid Nanoparticles for Oral Delivery of Oxyresveratrol: Effect of the Formulation Parameters on the Physicochemical Properties and in vitro Release </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yaowaporn%20Sangsen">Yaowaporn Sangsen</a>, <a href="https://publications.waset.org/abstracts/search?q=Kittisak%20Likhitwitayawuid"> Kittisak Likhitwitayawuid</a>, <a href="https://publications.waset.org/abstracts/search?q=Boonchoo%20Sritularak"> Boonchoo Sritularak</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamonthip%20Wiwattanawongsa"> Kamonthip Wiwattanawongsa</a>, <a href="https://publications.waset.org/abstracts/search?q=Ruedeekorn%20Wiwattanapatapee"> Ruedeekorn Wiwattanapatapee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Novel solid lipid nanoparticles (SLNs) were developed to improve oral bioavailability of oxyresveratrol (OXY). The SLNs were prepared by a high speed homogenization technique, at an effective speed and time, using Compritol® 888 ATO (5% w/w) as the solid lipid. The appropriate weight proportions (0.3% w/w) of OXY affected the physicochemical properties of blank SLNs. The effects of surfactant types on the properties of the formulations such as particle size and entrapment efficacy were also investigated. Conclusively, Tween 80 combined with soy lecithin was the most appropriate surfactant to stabilize OXY-loaded SLNs. The mean particle size of the optimized formulation was 134.40 ± 0.57 nm. In vitro drug release study, the selected S2 formulation showed a retarded release profile for OXY with no initial burst release compared to OXY suspension in the simulated gastrointestinal fluids. Therefore, these SLNs could provide a suitable system to develop for the oral OXY delivery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticles" title="solid lipid nanoparticles">solid lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical%20properties" title=" physicochemical properties"> physicochemical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20drug%20release" title=" in vitro drug release"> in vitro drug release</a>, <a href="https://publications.waset.org/abstracts/search?q=oxyresveratrol" title=" oxyresveratrol"> oxyresveratrol</a> </p> <a href="https://publications.waset.org/abstracts/3625/novel-solid-lipid-nanoparticles-for-oral-delivery-of-oxyresveratrol-effect-of-the-formulation-parameters-on-the-physicochemical-properties-and-in-vitro-release" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3625.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">397</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3229</span> Anonymous Gel-Fluid Transition of Solid Supported Lipids</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asma%20Poursoroush">Asma Poursoroush</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Solid-supported lipid bilayers are often used as a simple model for studies of biological membranes. The presence of a solid substrate that interacts attractively with lipid head-groups is expected to affect the phase behavior of the supported bilayer. Molecular dynamics simulations of a coarse-grained model are thus performed to investigate the phase behavior of supported one-component lipid bilayer membranes. Our results show that the attraction of the lipid head groups to the substrate leads to a phase behavior that is different from that of a free standing lipid bilayer. In particular, we found that the phase behaviors of the two leaflets are decoupled in the presence of a substrate. The proximal leaflet undergoes a clear gel-to-fluid phase transition at a temperature lower than that of a free standing bilayer, and that decreases with increasing strength of the substrate-lipid attraction. The distal leaflet, however, undergoes a change from a homogeneous liquid phase at high temperatures to a heterogeneous state consisting of small liquid and gel domains, with the average size of the gel domains that increases with decreasing temperature. While the chain order parameter of the proximal leaflet clearly shows a gel-fluid phase transition, the chain order parameter of the distal leaflet does not exhibit a clear phase transition. The decoupling in the phase behavior of the two leaflets is due to a non-symmteric lipid distribution in the two leaflets resulting from the presence of the substrate. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=membrane" title="membrane">membrane</a>, <a href="https://publications.waset.org/abstracts/search?q=substrate" title=" substrate"> substrate</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20dynamics" title=" molecular dynamics"> molecular dynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=simulation" title=" simulation"> simulation</a> </p> <a href="https://publications.waset.org/abstracts/78473/anonymous-gel-fluid-transition-of-solid-supported-lipids" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78473.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">195</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3228</span> Development of capsaicin-loaded nanostructured lipid carriers for topical application</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kwanputtha%20Arunprasert">Kwanputtha Arunprasert</a>, <a href="https://publications.waset.org/abstracts/search?q=Chaiyakarn%20Pornpitchanarong"> Chaiyakarn Pornpitchanarong</a>, <a href="https://publications.waset.org/abstracts/search?q=Praneet%20Opanasopit"> Praneet Opanasopit</a>, <a href="https://publications.waset.org/abstracts/search?q="></a>, <a href="https://publications.waset.org/abstracts/search?q=Prasopchai%20Patrojanasophon">Prasopchai Patrojanasophon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Capsaicin, a recently FDA-approved drug for the topical treatment of neuropathic pain, is associated with several side effects like burning sensation and erythema leading to severe skin irritation and poor patient compliance. These unwanted side effects are due to the rapid penetration of capsaicin into the epidermis and low permeation to the dermis layer. The purpose of this study was to develop nanostructured lipid carriers (NLCs) that entrapped capsaicin for reducing dermal irritation. Solid lipid (glyceryl monostearate (GM), cetyl palmitate (CP), cetyl alcohol (COH), stearic acid (SA), and stearyl alcohol (SOH)) and surfactant (Tween®80, Tween®20, and Span®20) were varied to obtained optimal capsaicin-loaded NLCs. The formulation using CP as solid lipid and Tween®80 as a surfactant (F2) demonstrated the smallest size, excellent colloidal stability, and narrow range distribution of the particles as being analyzed using Zetasizer. The obtained capsaicin-loaded NLCs were then characterized by entrapment efficiency (EE) and loading capacity (LC). The release characteristics followed Higuchi kinetics, and the prolonged capsaicin release may result in the reduction in skin irritation. These results could demonstrate the potentials of capsaicinloaded lipid-based nanoparticles for topical drug delivery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=capsaicin" title="capsaicin">capsaicin</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid-based%20nanoparticles" title=" lipid-based nanoparticles"> lipid-based nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=nanostructured%20lipid%20carriers" title=" nanostructured lipid carriers"> nanostructured lipid carriers</a>, <a href="https://publications.waset.org/abstracts/search?q=topical%20drug%20delivery%20system" title=" topical drug delivery system"> topical drug delivery system</a> </p> <a href="https://publications.waset.org/abstracts/179761/development-of-capsaicin-loaded-nanostructured-lipid-carriers-for-topical-application" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179761.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3227</span> Effect of Nanoparticle Diameter of Nano-Fluid on Average Nusselt Number in the Chamber</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Ghafouri">A. Ghafouri</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Pourmahmoud"> N. Pourmahmoud</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Mirzaee"> I. Mirzaee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this numerical study, effects of using Al2O3-water nanofluid on the rate of heat transfer have been investigated numerically. The physical model is a square enclosure with insulated top and bottom horizontal walls while the vertical walls are kept at different constant temperatures. Two appropriate models are used to evaluate the viscosity and thermal conductivity of nanofluid. The governing stream-vorticity equations are solved using a second order central finite difference scheme, coupled to the conservation of mass and energy. The study has been carried out for the nanoparticle diameter 30, 60, and 90 nm and the solid volume fraction 0 to 0.04. Results are presented by average Nusselt number and normalized Nusselt number in the different range of φ and D for mixed convection dominated regime. It is found that different heat transfer rate is predicted when the effect of nanoparticle diameter is taken into account. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanofluid" title="nanofluid">nanofluid</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticle%20diameter" title=" nanoparticle diameter"> nanoparticle diameter</a>, <a href="https://publications.waset.org/abstracts/search?q=heat%20transfer%20enhancement" title=" heat transfer enhancement"> heat transfer enhancement</a>, <a href="https://publications.waset.org/abstracts/search?q=square%20enclosure" title=" square enclosure"> square enclosure</a>, <a href="https://publications.waset.org/abstracts/search?q=Nusselt%20number" title=" Nusselt number"> Nusselt number</a> </p> <a href="https://publications.waset.org/abstracts/33124/effect-of-nanoparticle-diameter-of-nano-fluid-on-average-nusselt-number-in-the-chamber" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/33124.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">395</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3226</span> Spinach Lipid Extract as an Alternative Flow Aid for Fat Suspensions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nizaha%20Juhaida%20Mohamad">Nizaha Juhaida Mohamad</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20Gray"> David Gray</a>, <a href="https://publications.waset.org/abstracts/search?q=Bettina%20Wolf"> Bettina Wolf</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chocolate is a material composite with a high fraction of solid particles dispersed in a fat phase largely composed of cocoa butter. Viscosity properties of chocolate can be manipulated by the amount of fat - increased levels of fat lead to lower viscosity. However, a high content of cocoa butter can increase the cost of the chocolate and instead surfactants are used to manipulate viscosity behaviour. Most commonly, lecithin and polyglycerol polyricinoleate (PGPR) are used. Lecithin is a natural lipid emulsifier which is based on phospholipids while PGPR is a chemically produced emulsifier which based on the long continuous chain of ricinoleic acid. Lecithin and PGPR act to lower the viscosity and yield stress, respectively. Recently, natural lipid emulsifiers based on galactolipid as the functional ingredient have become of interest. Spinach lipid is found to have a high amount of galactolipid, specifically MGDG and DGDG. The aim of this research is to explore the influence of spinach lipid in comparison with PGPR and lecithin on the rheological properties of sugar/oil suspensions which serve as chocolate model system. For that purpose, icing sugar was dispersed from 40%, 45% and 50% (w/w) in oil which has spinach lipid at concentrations from 0.1 – 0.7% (w/w). Based on viscosity at 40 s-1 and yield value reported as shear stress measured at 5 s-1, it was found that spinach lipid shows viscosity reducing and yield stress lowering effects comparable to lecithin and PGPR, respectively. This characteristic of spinach lipid demonstrates great potential for it to act as single natural lipid emulsifier in chocolate. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chocolate%20viscosity" title="chocolate viscosity">chocolate viscosity</a>, <a href="https://publications.waset.org/abstracts/search?q=lecithin" title=" lecithin"> lecithin</a>, <a href="https://publications.waset.org/abstracts/search?q=polyglycerol%20polyricinoleate%20%28PGPR%29" title=" polyglycerol polyricinoleate (PGPR)"> polyglycerol polyricinoleate (PGPR)</a>, <a href="https://publications.waset.org/abstracts/search?q=spinach%20lipid" title=" spinach lipid"> spinach lipid</a> </p> <a href="https://publications.waset.org/abstracts/46928/spinach-lipid-extract-as-an-alternative-flow-aid-for-fat-suspensions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46928.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">248</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3225</span> Fabrication of Ligand Coated Lipid-Based Nanoparticles for Synergistic Treatment of Autoimmune Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asiya%20Mahtab">Asiya Mahtab</a>, <a href="https://publications.waset.org/abstracts/search?q=Sushama%20Talegaonkar"> Sushama Talegaonkar </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The research is aimed at developing targeted lipid-based nanocarrier systems of chondroitin sulfate (CS) to deliver an antirheumatic drug to the inflammatory site in arthritic paw. Lipid-based nanoparticle (TEF-lipo) was prepared by using a thin-film hydration method. The coating of prepared drug-loaded nanoparticles was done by the ionic interaction mechanism. TEF-lipo and CS-coated lipid nanoparticle (CS-lipo) were characterized for mean droplet size, zeta potential, and surface morphology. TEF-lipo and CS-lipo were further subjected to in vitro cell line studies on RAW 264.7 murine macrophage, U937, and MG 63 cell lines. The pharmacodynamic study was performed to establish the effectiveness of the prepared lipid-based conventional and targeted nanoparticles in comparison to pure drugs. Droplet size and zeta potential of TEF-lipo were found to be 128. 92 ± 5.42 nm and +12.6 ± 1.2 mV. It was observed that after the coating of TEF-lipo with CS, particle size increased to 155.6± 2.12 nm and zeta potential changed to -10.2± 1.4mV. Transmission electron microscopic analysis revealed that the nanovesicles were uniformly dispersed and detached from each other. Formulations followed sustained release pattern up to 24 h. Results of cell line studies ind icated that CS-lipo formulation showed the highest cytotoxic potential, thereby proving its enhanced ability to kill the RAW 264.7 murine macrophage and U937 cells when compared with other formulations. It is clear from our in vivo pharmacodynamic results that targeted nanocarriers had a higher inhibitory effect on arthritis progression than nontargeted nanocarriers or free drugs. Results demonstrate that this approach will provide effective treatment for rheumatoid arthritis, and CS served as a potential prophylactic against the advancement of cartilage degeneration. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adjuvant%20induced%20arthritis" title="adjuvant induced arthritis">adjuvant induced arthritis</a>, <a href="https://publications.waset.org/abstracts/search?q=chondroitin%20sulfate" title=" chondroitin sulfate"> chondroitin sulfate</a>, <a href="https://publications.waset.org/abstracts/search?q=rheumatoid%20arthritis" title=" rheumatoid arthritis"> rheumatoid arthritis</a>, <a href="https://publications.waset.org/abstracts/search?q=teriflunomide" title=" teriflunomide"> teriflunomide</a> </p> <a href="https://publications.waset.org/abstracts/116801/fabrication-of-ligand-coated-lipid-based-nanoparticles-for-synergistic-treatment-of-autoimmune-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116801.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3224</span> Development and Characterization of Topical 5-Fluorouracil Solid Lipid Nanoparticles for the Effective Treatment of Non-Melanoma Skin Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sudhir%20Kumar">Sudhir Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20R.%20Sinha"> V. R. Sinha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The topical and systemic toxicity associated with present nonmelanoma skin cancer (NMSC) treatment therapy using 5-Fluorouracil (5-FU) make it necessary to develop a novel delivery system having lesser toxicity and better control over drug release. Solid lipid nanoparticles offer many advantages like: controlled and localized release of entrapped actives, nontoxicity, and better tolerance. Aim:-To investigate safety and efficacy of 5-FU loaded solid lipid nanoparticles as a topical delivery system for the treatment of nonmelanoma skin cancer. Method: Topical solid lipid nanoparticles of 5-FU were prepared using Compritol 888 ATO (Glyceryl behenate) as lipid component and pluronic F68 (Poloxamer 188), Tween 80 (Polysorbate 80), Tyloxapol (4-(1,1,3,3-Tetramethylbutyl) phenol polymer with formaldehyde and oxirane) as surfactants. The SLNs were prepared with emulsification method. Different formulation parameters viz. type and ratio of surfactant, ratio of lipid and ratio of surfactant:lipid were investigated on particle size and drug entrapment efficiency. Results: Characterization of SLNs like–Transmission Electron Microscopy (TEM), Differential Scannig calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Particle size determination, Polydispersity index, Entrapment efficiency, Drug loading, ex vivo skin permeation and skin retention studies, skin irritation and histopathology studies were performed. TEM results showed that shape of SLNs was spherical with size range 200-500nm. Higher encapsulation efficiency was obtained for batches having higher concentration of surfactant and lipid. It was found maximum 64.3% for SLN-6 batch with size of 400.1±9.22 nm and PDI 0.221±0.031. Optimized SLN batches and marketed 5-FU cream were compared for flux across rat skin and skin drug retention. The lesser flux and higher skin retention was obtained for SLN formulation in comparison to topical 5-FU cream, which ensures less systemic toxicity and better control of drug release across skin. Chronic skin irritation studies lacks serious erythema or inflammation and histopathology studies showed no significant change in physiology of epidermal layers of rat skin. So, these studies suggest that the optimized SLN formulation is efficient then marketed cream and safer for long term NMSC treatment regimens. Conclusion: Topical and systemic toxicity associated with long-term use of 5-FU, in the treatment of NMSC, can be minimized with its controlled release with significant drug retention with minimal flux across skin. The study may provide a better alternate for effective NMSC treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=5-FU" title="5-FU">5-FU</a>, <a href="https://publications.waset.org/abstracts/search?q=topical%20formulation" title=" topical formulation"> topical formulation</a>, <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticles" title=" solid lipid nanoparticles"> solid lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=non%20melanoma%20skin%20cancer" title=" non melanoma skin cancer"> non melanoma skin cancer</a> </p> <a href="https://publications.waset.org/abstracts/20727/development-and-characterization-of-topical-5-fluorouracil-solid-lipid-nanoparticles-for-the-effective-treatment-of-non-melanoma-skin-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20727.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">516</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3223</span> Lipid-Chitosan Hybrid Nanoparticles for Controlled Delivery of Cisplatin</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Muzamil%20Khan">Muhammad Muzamil Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Asadullah%20Madni"> Asadullah Madni</a>, <a href="https://publications.waset.org/abstracts/search?q=Nina%20Filipczek"> Nina Filipczek</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiayi%20Pan"> Jiayi Pan</a>, <a href="https://publications.waset.org/abstracts/search?q=Nayab%20Tahir"> Nayab Tahir</a>, <a href="https://publications.waset.org/abstracts/search?q=Hassan%20Shah"> Hassan Shah</a>, <a href="https://publications.waset.org/abstracts/search?q=Vladimir%20Torchilin"> Vladimir Torchilin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lipid-polymer hybrid nanoparticles (LPHNP) are delivery systems for controlled drug delivery at tumor sites. The superior biocompatible properties of lipid and structural advantages of polymer can be obtained via this system for controlled drug delivery. In the present study, cisplatin-loaded lipid-chitosan hybrid nanoparticles were formulated by the single step ionic gelation method based on ionic interaction of positively charged chitosan and negatively charged lipid. Formulations with various chitosan to lipid ratio were investigated to obtain the optimal particle size, encapsulation efficiency, and controlled release pattern. Transmission electron microscope and dynamic light scattering analysis demonstrated a size range of 181-245 nm and a zeta potential range of 20-30 mV. Compatibility among the components and the stability of formulation were demonstrated with FTIR analysis and thermal studies, respectively. The therapeutic efficacy and cellular interaction of cisplatin-loaded LPHNP were investigated using in vitro cell-based assays in A2780/ADR ovarian carcinoma cell line. Additionally, the cisplatin loaded LPHNP exhibited a low toxicity profile in rats. The in-vivo pharmacokinetics study also proved a controlled delivery of cisplatin with enhanced mean residual time and half-life. Our studies suggested that the cisplatin-loaded LPHNP being a promising platform for controlled delivery of cisplatin in cancer therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cisplatin" title="cisplatin">cisplatin</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid-polymer%20hybrid%20nanoparticle" title=" lipid-polymer hybrid nanoparticle"> lipid-polymer hybrid nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=chitosan" title=" chitosan"> chitosan</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20cell%20line%20study" title=" in vitro cell line study"> in vitro cell line study</a> </p> <a href="https://publications.waset.org/abstracts/108442/lipid-chitosan-hybrid-nanoparticles-for-controlled-delivery-of-cisplatin" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/108442.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">130</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3222</span> Synthesis of Nanoparticle Mordenite Zeolite for Dimethyl Ether Carbonylation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhang%20Haitao">Zhang Haitao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The different size of nanoparticle mordenite zeolites were prepared by adding different soft template during hydrothermal process for carbonylation of dimethyl ether (DME) to methyl acetate (MA). The catalysts were characterized by X-ray diffraction, Ar adsorption-desorption, high-resolution transmission electron microscopy, NH3-temperature programmed desorption, scanning electron microscopy and Thermogravimetric. The characterization results confirmed that mordenite zeolites with small nanoparticle showed more strong acid sites which was the active site for carbonylation thus promoting conversion of DME and MA selectivity. Furthermore, the nanoparticle mordenite had increased the mass transfer efficiency which could suppress the formation of coke. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanoparticle%20mordenite" title="nanoparticle mordenite">nanoparticle mordenite</a>, <a href="https://publications.waset.org/abstracts/search?q=carbonylation" title=" carbonylation"> carbonylation</a>, <a href="https://publications.waset.org/abstracts/search?q=dimethyl%20ether" title=" dimethyl ether"> dimethyl ether</a>, <a href="https://publications.waset.org/abstracts/search?q=methyl%20acetate" title=" methyl acetate"> methyl acetate</a> </p> <a href="https://publications.waset.org/abstracts/120694/synthesis-of-nanoparticle-mordenite-zeolite-for-dimethyl-ether-carbonylation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/120694.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3221</span> Formulation and Invivo Evaluation of Salmeterol Xinafoate Loaded MDI for Asthma Using Response Surface Methodology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Paresh%20Patel">Paresh Patel</a>, <a href="https://publications.waset.org/abstracts/search?q=Priya%20Patel"> Priya Patel</a>, <a href="https://publications.waset.org/abstracts/search?q=Vaidehi%20Sorathiya"> Vaidehi Sorathiya</a>, <a href="https://publications.waset.org/abstracts/search?q=Navin%20Sheth"> Navin Sheth</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of present work was to fabricate Salmeterol Xinafoate (SX) metered dose inhaler (MDI) for asthma and to evaluate the SX loaded solid lipid nanoparticles (SLNs) for pulmonary delivery. Solid lipid nanoparticles can be used to deliver particles to the lungs via MDI. A modified solvent emulsification diffusion technique was used to prepare Salmeterol Xinafoate loaded solid lipid nanoparticles by using compritol 888 ATO as lipid, tween 80 as surfactant, D-mannitol as cryoprotecting agent and L-leucine was used to improve aerosolization behaviour. Box-Behnken design was applied with 17 runs. 3-D surface response plots and contour plots were drawn and optimized formulation was selected based on minimum particle size and maximum % EE. % yield, in vitro diffusion study, scanning electron microscopy, X-ray diffraction, DSC, FTIR also characterized. Particle size, zeta potential analyzed by Zetatrac particle size analyzer and aerodynamic properties was carried out by cascade impactor. Pre convulsion time was examined for control group, treatment group and compare with marketed group. MDI was evaluated for leakage test, flammability test, spray test and content per puff. By experimental design, particle size and % EE found to be in range between 119-337 nm and 62.04-76.77% by solvent emulsification diffusion technique. Morphologically, particles have spherical shape and uniform distribution. DSC & FTIR study showed that no interaction between drug and excipients. Zeta potential shows good stability of SLNs. % respirable fraction found to be 52.78% indicating reach to the deep part of lung such as alveoli. Animal study showed that fabricated MDI protect the lungs against histamine induced bronchospasm in guinea pigs. MDI showed sphericity of particle in spray pattern, 96.34% content per puff and non-flammable. SLNs prepared by Solvent emulsification diffusion technique provide desirable size for deposition into the alveoli. This delivery platform opens up a wide range of treatment application of pulmonary disease like asthma via solid lipid nanoparticles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=salmeterol%20xinafoate" title="salmeterol xinafoate">salmeterol xinafoate</a>, <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticles" title=" solid lipid nanoparticles"> solid lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=box-behnken%20design" title=" box-behnken design"> box-behnken design</a>, <a href="https://publications.waset.org/abstracts/search?q=solvent%20emulsification%20diffusion%20technique" title=" solvent emulsification diffusion technique"> solvent emulsification diffusion technique</a>, <a href="https://publications.waset.org/abstracts/search?q=pulmonary%20delivery" title=" pulmonary delivery"> pulmonary delivery</a> </p> <a href="https://publications.waset.org/abstracts/34847/formulation-and-invivo-evaluation-of-salmeterol-xinafoate-loaded-mdi-for-asthma-using-response-surface-methodology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34847.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">451</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3220</span> Formulation and Ex Vivo Evaluation of Solid Lipid Nanoparticles Based Hydrogel for Intranasal Drug Delivery</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pramod%20Jagtap">Pramod Jagtap</a>, <a href="https://publications.waset.org/abstracts/search?q=Kisan%20Jadhav"> Kisan Jadhav</a>, <a href="https://publications.waset.org/abstracts/search?q=Neha%20Dand"> Neha Dand</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Risperidone (RISP) is an antipsychotic agent and has low water solubility and nontargeted delivery results in numerous side effects. Hence, an attempt was made to develop SLNs hydrogel for intranasal delivery of RISP to achieve maximum bioavailability and reduction of side effects. RISP loaded SLNs composed of 1.65% (w/v) lipid mass were produced by high shear homogenization (HSH) coupled ultrasound (US) method using glyceryl monostearate (GMS) or Imwitor 900K (solid lipid). The particles were loaded with 0.2% (w/v) of the RISP & surface-tailored with a 2.02% (w/v) non-ionic surfactant Tween® 80. Optimization was done using 32 factorial design using Design Expert® software. The prepared SLNs dispersion incorporated into Polycarbophil AA1 hydrogel (0.5% w/v). The final gel formulation was evaluated for entrapment efficiency, particle size, rheological properties, X ray diffraction, in vitro diffusion, ex vivo permeation using sheep nasal mucosa and histopathological studies for nasocilliary toxicity. The entrapment efficiency of optimized SLNs was found to be 76 ± 2 %, polydispersity index <0.3., particle size 278 ± 5 nm. This optimized batch was incorporated into hydrogel. The pH was found to be 6.4 ± 0.14. The rheological behaviour of hydrogel formulation revealed no thixotropic behaviour. In histopathology study, there was no nasocilliary toxicity observed in nasal mucosa after ex vivo permeation. X-ray diffraction data shows drug was in amorphous form. Ex vivo permeation study shows controlled release profile of drug. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ex%20vivo" title="ex vivo">ex vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=particle%20size" title=" particle size"> particle size</a>, <a href="https://publications.waset.org/abstracts/search?q=risperidone" title=" risperidone"> risperidone</a>, <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticles" title=" solid lipid nanoparticles"> solid lipid nanoparticles</a> </p> <a href="https://publications.waset.org/abstracts/20704/formulation-and-ex-vivo-evaluation-of-solid-lipid-nanoparticles-based-hydrogel-for-intranasal-drug-delivery" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20704.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">418</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3219</span> Effect of Nanoparticle Addition in the Urea-Formaldehyde Resin on the Formaldehyde Emission from MDF</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sezen%20Gurdag">Sezen Gurdag</a>, <a href="https://publications.waset.org/abstracts/search?q=Ayse%20Ebru%20Akin"> Ayse Ebru Akin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is a growing concern all over the world on the health effect of the formaldehyde emission coming from the adhesive used in the MDF production. In this research, we investigated the effect of nanoparticle addition such as nanoclay and halloysite into urea-formadehyde resin on the total emitted formaldehyde from MDF plates produced using the resin modified as such. First, the curing behavior of the resin was studied by monitoring the pH, curing time, solid content, density and viscosity of the modified resin in comparison to the reference resin with no added nanoparticle. The dosing of the nanoparticle in the dry resin was kept at 1wt%, 3wt% or 5wt%. Consecutively, the resin was used in the production of 50X50 cm MDF samples using laboratory scale press line with full automation system. Modulus of elasticity, bending strength, internal bonding strength, water absorption were also measured in addition to the main interested parameter formaldehyde emission levels which is determined via spectrometric technique following an extraction procedure. Threshold values for nanoparticle dosing levels were determined to be 5wt% for both nanoparticles. However, the reinforcing behavior was observed to be occurring at different levels in comparison to the reference plates with each nanoparticle such that the level of reinforcement with nanoclay was shown to be more favorable than the addition of halloysite due to higher surface area available with the former. In relation, formaldehyde emission levels were observed to be following a similar trend where addition of 5wt% nanoclay into the urea-formaldehyde adhesive helped decrease the formaldehyde emission up to 40% whereas addition of halloysite at its threshold level demonstrated as the same level, i.e., 5wt%, produced an improvement of 18% only. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=halloysite" title="halloysite">halloysite</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoclay" title=" nanoclay"> nanoclay</a>, <a href="https://publications.waset.org/abstracts/search?q=fiberboard" title=" fiberboard"> fiberboard</a>, <a href="https://publications.waset.org/abstracts/search?q=urea-formaldehyde%20adhesive" title=" urea-formaldehyde adhesive"> urea-formaldehyde adhesive</a> </p> <a href="https://publications.waset.org/abstracts/102152/effect-of-nanoparticle-addition-in-the-urea-formaldehyde-resin-on-the-formaldehyde-emission-from-mdf" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/102152.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3218</span> Lipid from Activated Sludge as a Feedstock for the Production of Biodiesel</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ifeanyichukwu%20Edeh">Ifeanyichukwu Edeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Tim%20Overton"> Tim Overton</a>, <a href="https://publications.waset.org/abstracts/search?q=Steve%20Bowra"> Steve Bowra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is increasing interest in utilising low grade or waste biomass for the production of renewable bioenergy vectors i.e. waste to energy. In this study we have chosen to assess, activated sludge, which is a microbial biomass generated during the second stage of waste water treatment as a source of lipid for biodiesel production. To date a significant proportion of biodiesel is produced from used cooking oil and animal fats. It was reasoned that if activated sludge proved a viable feedstock it has the potential to support increase biodiesel production capacity. Activated sludge was obtained at different times of the year and from two different sewage treatment works in the UK. The biomass within the activated sludge slurry was recovered by filtration and the total weight of material calculated by combining the dry weight of the total suspended solid (TSS) and the total dissolved solid (TDS) fractions. Total lipids were extracted from the TSS and TDS using solvent extraction (Folch methods). The classes of lipids within the total lipid extract were characterised using high performance thin layer chromatography (HPTLC) by referencing known standards. The fatty acid profile and content of the lipid extract were determined using acid mediated-methanolysis to obtain fatty acid methyl esters (FAMEs) which were analysed by gas chromatography and HPTLC. The results showed that there were differences in the total biomass content in the activated sludge collected from different sewage works. Lipid yields from TSS obtained from both sewage treatment works differed according to the time of year (between 3.0 and 7.4 wt. %). The lipid yield varied slightly within the same source of biomass but more widely between the two sewage treatment works. The neutral lipid classes identified were acylglycerols, free fatty acids, sterols and wax esters while the phospholipid class included phosphatidylcholine, lysophosphatidycholine, phosphatidylethanolamine and phosphatidylinositol. The fatty acid profile revealed the presence of palmitic acid, palmitoleic acid, linoleic acid, oleic acid and stearic acid and that unsaturated fatty acids were the most abundant. Following optimisation, the FAME yield was greater than 10 wt. % which was required to have an economic advantage in biodiesel production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=activated%20sludge" title="activated sludge">activated sludge</a>, <a href="https://publications.waset.org/abstracts/search?q=biodiesel" title=" biodiesel"> biodiesel</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid" title=" lipid"> lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=methanolysis" title=" methanolysis"> methanolysis</a> </p> <a href="https://publications.waset.org/abstracts/37751/lipid-from-activated-sludge-as-a-feedstock-for-the-production-of-biodiesel" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37751.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">472</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3217</span> Effect of Nitrogen and Carbon Sources on Growth and Lipid Production from Mixotrophic Growth of Chlorella sp. KKU-S2</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ratanaporn%20Leesing">Ratanaporn Leesing</a>, <a href="https://publications.waset.org/abstracts/search?q=Thidarat%20Papone"> Thidarat Papone</a>, <a href="https://publications.waset.org/abstracts/search?q=Mutiyaporn%20Puangbut"> Mutiyaporn Puangbut</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mixotrophic cultivation of the isolated freshwater microalgae Chlorella sp. KKU-S2 in batch shake flask for biomass and lipid productions, different concentration of glucose as carbon substrate, different nitrogen source and concentrations were investigated. Using 1.0g/L of NaNO3 as nitrogen source, the maximum biomass yield of 10.04g/L with biomass productivity of 1.673g/L d was obtained using 40g/L glucose, while a biomass of 7.09, 8.55 and 9.45g/L with biomass productivity of 1.182, 1.425 and 1.575g/L d were found at 20, 30 and 50g/L glucose, respectively. The maximum lipid yield of 3.99g/L with lipid productivity of 0.665g/L d was obtained when 40g/L glucose was used. Lipid yield of 1.50, 3.34 and 3.66g/L with lipid productivity of 0.250, 0.557 and 0.610g/L d were found when using the initial concentration of glucose at 20, 30 and 50g/L, respectively. Process product yield (YP/S) of 0.078, 0.119, 0.158 and 0.094 were observed when glucose concentration was 20, 30, 40 and 50 g/L, respectively. The results obtained from the study shows that mixotrophic culture of Chlorella sp. KKU-S2 is a desirable cultivation process for microbial lipid and biomass production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mixotrophic%20cultivation" title="mixotrophic cultivation">mixotrophic cultivation</a>, <a href="https://publications.waset.org/abstracts/search?q=microalgal%20lipid" title=" microalgal lipid"> microalgal lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=Chlorella%20sp.%20KKU-S2" title=" Chlorella sp. KKU-S2"> Chlorella sp. KKU-S2</a> </p> <a href="https://publications.waset.org/abstracts/5171/effect-of-nitrogen-and-carbon-sources-on-growth-and-lipid-production-from-mixotrophic-growth-of-chlorella-sp-kku-s2" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5171.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">340</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3216</span> Preparation and in vivo Assessment of Nystatin-Loaded Solid Lipid Nanoparticles for Topical Delivery against Cutaneous Candidiasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rawia%20M.%20Khalil">Rawia M. Khalil</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20A.%20Abd%20El%20Rahman"> Ahmed A. Abd El Rahman</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahfouz%20A.%20Kassem"> Mahfouz A. Kassem</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20S.%20El%20Ridi"> Mohamed S. El Ridi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mona%20M.%20Abou%20Samra"> Mona M. Abou Samra</a>, <a href="https://publications.waset.org/abstracts/search?q=Ghada%20E.%20A.%20Awad"> Ghada E. A. Awad</a>, <a href="https://publications.waset.org/abstracts/search?q=Soheir%20S.%20Mansy"> Soheir S. Mansy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Solid lipid nanoparticles (SLNs) have gained great attention for the topical treatment of skin associated fungal infection as they facilitate the skin penetration of loaded drugs. Our work deals with the preparation of nystatin loaded solid lipid nanoparticles (NystSLNs) using the hot homogenization and ultrasonication method. The prepared NystSLNs were characterized in terms of entrapment efficiency, particle size, zeta potential, transmission electron microscopy, differential scanning calorimetry, rheological behavior and in vitro drug release. A stability study for 6 months was performed. A microbiological study was conducted in male rats infected with Candida albicans, by counting the colonies and examining the histopathological changes induced on the skin of infected rats. The results showed that SLNs dispersions are spherical in shape with particle size ranging from 83.26±11.33 to 955.04±1.09 nm. The entrapment efficiencies are ranging from 19.73±1.21 to 72.46±0.66% with zeta potential ranging from -18.9 to -38.8 mV and shear-thinning rheological Behavior. The stability studies done for 6 months showed that nystatin (Nyst) is a good candidate for topical SLN formulations. A least number of colony forming unit/ ml (cfu/ml) was recorded for the selected NystSLN compared to the drug solution and the commercial Nystatin® cream present in the market. It can be fulfilled from this work that SLNs provide a good skin targeting effect and may represent promising carrier for topical delivery of Nyst offering the sustained release and maintaining the localized effect, resulting in an effective treatment of cutaneous fungal infection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=candida%20infections" title="candida infections">candida infections</a>, <a href="https://publications.waset.org/abstracts/search?q=hot%20homogenization" title=" hot homogenization"> hot homogenization</a>, <a href="https://publications.waset.org/abstracts/search?q=nystatin" title=" nystatin"> nystatin</a>, <a href="https://publications.waset.org/abstracts/search?q=solid%20lipid%20nanoparticles" title=" solid lipid nanoparticles"> solid lipid nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=stability" title=" stability"> stability</a>, <a href="https://publications.waset.org/abstracts/search?q=topical%20delivery" title=" topical delivery"> topical delivery</a> </p> <a href="https://publications.waset.org/abstracts/3539/preparation-and-in-vivo-assessment-of-nystatin-loaded-solid-lipid-nanoparticles-for-topical-delivery-against-cutaneous-candidiasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3539.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">393</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3215</span> Total Lipid of Mutant Synechococcus sp. PCC 7002</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Azlin%20S%20Azmi">Azlin S Azmi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mus%E2%80%99ab%20Zainal"> Mus’ab Zainal</a>, <a href="https://publications.waset.org/abstracts/search?q=Sarina%20Sulaiman"> Sarina Sulaiman</a>, <a href="https://publications.waset.org/abstracts/search?q=Azura%20Amid"> Azura Amid</a>, <a href="https://publications.waset.org/abstracts/search?q=Zaki%20Zainudin"> Zaki Zainudin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microalgae lipid is a promising feedstock for biodiesel production. The objective of this work was to study growth factors affecting marine mutant Synechococcus sp. (PCC 7002) for high lipid production. Four growth factors were investigated; nitrogen-phosporus-potassium (NPK) concentration, light intensity, temperature and NaNO3 concentration on mutant strain growth and lipid production were studied. Design Expert v8.0 was used to design the experimental and analyze the data. The experimental design selected was Min-Run Res IV which consists of 12 runs and the response surfaces measured were specific growth rate and lipid concentration. The extraction of lipid was conducted by chloroform/methanol solvents system. Based on the study, mutant Synechococcus sp. PCC 7002 gave the highest specific growth rate of 0.0014 h-1 at 0% NPK, 2500 lux, 40oC and 0% NaNO3. On the other hand, the highest lipid concentration was obtained at 0% NPK, 3500 lux, 30°C and 1% NaNO3. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cyanobacteria" title="Cyanobacteria">Cyanobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid" title=" lipid"> lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=mutant" title=" mutant"> mutant</a>, <a href="https://publications.waset.org/abstracts/search?q=marine%20Synechococcus%20sp.%20%28PCC%207002%29" title=" marine Synechococcus sp. (PCC 7002)"> marine Synechococcus sp. (PCC 7002)</a>, <a href="https://publications.waset.org/abstracts/search?q=specific%20growth%20rate" title=" specific growth rate"> specific growth rate</a> </p> <a href="https://publications.waset.org/abstracts/8067/total-lipid-of-mutant-synechococcus-sp-pcc-7002" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/8067.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">337</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3214</span> Protein and Lipid Extraction from Microalgae with Ultrasound Assisted Osmotic Shock Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nais%20Pinta%20Adetya">Nais Pinta Adetya</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Hadiyanto"> H. Hadiyanto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microalgae has a potential to be utilized as food and natural colorant. The microalgae components consists of three main parts, these are lipid, protein, and carbohydrate. Crucial step in producing lipid and protein from microalgae is extraction. Microalgae has high water level (70-90%), it causes drying process of biomass needs much more energy and also has potential to distract lipid and protein from microalgae. Extraction of lipid from wet biomass is able to take place efficiently with cell disruption of microalgae by osmotic shock method. In this study, osmotic shock method was going to be integrated with ultrasound to maximalize the extraction yield of lipid and protein from wet biomass Spirulina sp. with osmotic shock method assisted ultrasound. This study consisted of two steps, these were osmotic shock process toward wet biomass and ultrasound extraction assisted. NaCl solution was used as osmotic agent, with the variation of concentrations were 10%, 20%, and 30%. Extraction was conducted in 40°C for 20 minutes with frequency of ultrasound wave was 40kHz. The optimal yield of protein (2.7%) and (lipid 38%) were achieved at 20% osmotic agent concentration. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=extraction" title="extraction">extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid" title=" lipid"> lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=osmotic%20shock" title=" osmotic shock"> osmotic shock</a>, <a href="https://publications.waset.org/abstracts/search?q=protein" title=" protein"> protein</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound" title=" ultrasound"> ultrasound</a> </p> <a href="https://publications.waset.org/abstracts/76886/protein-and-lipid-extraction-from-microalgae-with-ultrasound-assisted-osmotic-shock-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76886.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">358</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3213</span> Superoxide Dismutase Activity of Male Rats after Administration of Extract and Nanoparticle of Ginger Torch Flower</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tresna%20Lestari">Tresna Lestari</a>, <a href="https://publications.waset.org/abstracts/search?q=Tita%20Nofianti"> Tita Nofianti</a>, <a href="https://publications.waset.org/abstracts/search?q=Ade%20Yeni%20Aprilia"> Ade Yeni Aprilia</a>, <a href="https://publications.waset.org/abstracts/search?q=Lilis%20Tuslinah"> Lilis Tuslinah</a>, <a href="https://publications.waset.org/abstracts/search?q=Ruswanto%20Ruswanto"> Ruswanto Ruswanto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nanoparticle formulation is often used to improve drug absorptivity, thus increasing the sharpness of the action. Ginger torch flower extract was formulated into nanoparticle form using poloxamer 1, 3 and 5%. The nanoparticle was then characterized by its particle size, polydispersity index, zeta potential, entrapment efficiency and morphological form by SEM. The result shows that nanoparticle formulations have particle size 134.7-193.1 nm, polydispersity index less than 0.5 for all formulations, zeta potential -41.0 - (-24.3) mV and entrapment efficiency 89.93-97.99 against flavonoid content with a soft surface and spherical form of particles. Methanolic extract of ginger torch flower could enhance superoxide dismutase activity by 1,3183 U/mL in male rats. Nanoparticle formulation of ginger torch extract is expected to increase the capability of the drug to enhance superoxide dismutase activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=superoxide%20dismutase" title="superoxide dismutase">superoxide dismutase</a>, <a href="https://publications.waset.org/abstracts/search?q=ginger%20torch%20flower" title=" ginger torch flower"> ginger torch flower</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticle" title=" nanoparticle"> nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=poloxamer" title=" poloxamer"> poloxamer</a> </p> <a href="https://publications.waset.org/abstracts/91992/superoxide-dismutase-activity-of-male-rats-after-administration-of-extract-and-nanoparticle-of-ginger-torch-flower" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91992.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3212</span> Cell Biomass and Lipid Productivities of Meyerella planktonica under Autotrophic and Heterotrophic Growth Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rory%20Anthony%20Hutagalung">Rory Anthony Hutagalung</a>, <a href="https://publications.waset.org/abstracts/search?q=Leonardus%20Widjaja"> Leonardus Widjaja</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microalgae Meyerella planktonica is a potential biofuel source because it can grow in bulk in either autotrophic or heterotrophic condition. However, the quantitative growth of this algal type is still low as it tends to precipitates on the bottom. Beside, the lipid concentration is still low when grown in autotrophic condition. In contrast, heterotrophic condition can enhance the lipid concentration. The combination of autotrophic condition and agitation treatment was conducted to increase the density of the culture. On the other hand, a heterotrophic condition was set up to raise the lipid production. A two-stage experiment was applied to increase the density at the first step and to increase the lipid concentration in the next step. The autotrophic condition resulted higher density but lower lipid concentration compared to heterotrophic one. The agitation treatment produced higher density in both autotrophic and heterotrophic conditions. The two-stage experiment managed to enhance the density during the autotrophic stage and the lipid concentration during the heterotrophic stage. The highest yield was performed by using 0.4% v/v glycerol as a carbon source (2.9±0.016 x 106 cells w/w) attained 7 days after the heterotrophic stage began. The lipid concentration was stable starting from day 7. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=agitation" title="agitation">agitation</a>, <a href="https://publications.waset.org/abstracts/search?q=glycerol" title=" glycerol"> glycerol</a>, <a href="https://publications.waset.org/abstracts/search?q=heterotrophic" title=" heterotrophic"> heterotrophic</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20productivity" title=" lipid productivity"> lipid productivity</a>, <a href="https://publications.waset.org/abstracts/search?q=Meyerella%20planktonica" title=" Meyerella planktonica"> Meyerella planktonica</a> </p> <a href="https://publications.waset.org/abstracts/29280/cell-biomass-and-lipid-productivities-of-meyerella-planktonica-under-autotrophic-and-heterotrophic-growth-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29280.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">337</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3211</span> Lipid-polymer Nanocarrier Platform Enables X-Ray Induced Photodynamic Therapy against Human Colorectal Cancer Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rui%20Sang">Rui Sang</a>, <a href="https://publications.waset.org/abstracts/search?q=Fei%20Deng"> Fei Deng</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexander%20Engel"> Alexander Engel</a>, <a href="https://publications.waset.org/abstracts/search?q=Ewa%20M.%20Goldys"> Ewa M. Goldys</a>, <a href="https://publications.waset.org/abstracts/search?q=Wei%20Deng"> Wei Deng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we brought together X-ray induced photodynamic therapy (X-PDT) and chemo-drug (5-FU) for the treatment on colorectal cancer cells. This was achieved by developing a lipid-polymer hybrid nanoparticle delivery system (FA-LPNPs-VP-5-FU). It was prepared by incorporating a photosensitizer (verteporfin), chemotherapy drug (5-FU), and a targeting moiety (folic acid) into one platform. The average size of these nanoparticles was around 100 nm with low polydispersity. When exposed to clinical doses of 4 Gy X-ray radiation, FA-LPNPs-VP-5-FU generated sufficient amounts of reactive oxygen species, triggering the apoptosis and necrosis pathway of cancer cells. Our combined X-PDT and chemo-drug strategy was effective in inhibiting cancer cells’ growth and proliferation. Cell cycle analyses revealed that our treatment induced G2/M and S phase arrest in HCT116 cells. Our results indicate that this combined treatment provides better antitumour effect in colorectal cancer cells than each of these modalities alone. This may offer a novel approach for effective colorectal cancer treatment with reduced off-target effect and drug toxicity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pdt" title="pdt">pdt</a>, <a href="https://publications.waset.org/abstracts/search?q=targeted%20lipid-polymer%20nanoparticles" title=" targeted lipid-polymer nanoparticles"> targeted lipid-polymer nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=verteporfin" title=" verteporfin"> verteporfin</a>, <a href="https://publications.waset.org/abstracts/search?q=colorectal%20cancer" title=" colorectal cancer"> colorectal cancer</a> </p> <a href="https://publications.waset.org/abstracts/164493/lipid-polymer-nanocarrier-platform-enables-x-ray-induced-photodynamic-therapy-against-human-colorectal-cancer-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164493.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3210</span> Formulation of Famotidine Solid Lipid Nanoparticles (SLN): Preparation, Evaluation and Release Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rachmat%20Mauludin">Rachmat Mauludin</a>, <a href="https://publications.waset.org/abstracts/search?q=Nurmazidah"> Nurmazidah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and purpose: Famotidine is an H2 receptor blocker. Absorption orally is rapid enough, but famotidine can be degraded by stomach acid causing dose reduction until 35.8% after 50 minutes. This drug also undergoes first-pass metabolism which reduced its bio availability only until 40-50%. To overcome these problems, Solid Lipid Nano particles (SLNs) as alternative delivery systems can be formulated. SLNs is a lipid-based drug delivery technology with 50-1000 nm particle size, where the drug incorporated into the bio compatible lipids and the lipid particles are stabilized using appropriate stabilizers. When the particle size is 200 nm or below, lipid containing famotidine can be absorbed through the lymphatic vessels to the subclavian vein, so first-pass metabolism can be avoided. Method: Famotidine SLNs with various compositions of stabilizer was prepared using a high-speed homogenization and sonication method. Then, the particle size distribution, zeta potential, entrapment efficiency, particle morphology and in vitro release profiles were evaluated. Optimization of sonication time also carried out. Result: Particle size of SLN by Particle Size Analyzer was in range 114.6 up to 455.267 nm. Ultrasonicated SLNs within 5 minutes generated smaller particle size than SLNs which was ultrasonicated for 10 and 15 minutes. Entrapment efficiency of SLNs were 74.17 up to 79.45%. Particle morphology of the SLNs was spherical and distributed individually. Release study of Famotidine revealed that in acid medium, 28.89 up to 80.55% of famotidine could be released after 2 hours. Nevertheless in basic medium, famotidine was released 40.5 up to 86.88% in the same period. Conclusion: The best formula was SLNs which stabilized by 4% Poloxamer 188 and 1 % Span 20, that had particle size 114.6 nm in diameter, 77.14% famotidine entrapped, and the particle morphology was spherical and distributed individually. SLNs with the best drug release profile was SLNs which stabilized by 4% Eudragit L 100-55 and 1% Tween 80 which had released 36.34 % in pH 1.2 solution, and 74.13% in pH 7.4 solution after 2 hours. The optimum sonication time was 5 minutes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=famotodine" title="famotodine">famotodine</a>, <a href="https://publications.waset.org/abstracts/search?q=SLN" title=" SLN"> SLN</a>, <a href="https://publications.waset.org/abstracts/search?q=high%20speed%20homogenization" title=" high speed homogenization"> high speed homogenization</a>, <a href="https://publications.waset.org/abstracts/search?q=particle%20size" title=" particle size"> particle size</a>, <a href="https://publications.waset.org/abstracts/search?q=release%20study" title=" release study"> release study</a> </p> <a href="https://publications.waset.org/abstracts/20331/formulation-of-famotidine-solid-lipid-nanoparticles-sln-preparation-evaluation-and-release-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20331.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">859</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3209</span> Time-Course Lipid Accumulation and Transcript Analyses of Lipid Biosynthesis Gene of Chlorella sp.3 under Nitrogen Limited Condition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jyoti%20Singh">Jyoti Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Swati%20Dubey"> Swati Dubey</a>, <a href="https://publications.waset.org/abstracts/search?q=Mukta%20Singh"> Mukta Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20P.%20Singh"> R. P. Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The freshwater microalgae Chlorella sp. is alluring considerable interest as a source for biofuel production due to its fast growth rate and high lipid content. Under nitrogen limited conditions, they can accumulate significant amounts of lipids. Thus, it is important to gain insight into the molecular mechanism of their lipid metabolism. In this study under nitrogen limited conditions, regular pattern of growth characteristics lipid accumulation and gene expression analysis of key regulatory genes of lipid biosynthetic pathway were carried out in microalgae Chlorella sp 3. Our results indicated that under nitrogen limited conditions there is a significant increase in the lipid content and lipid productivity, achieving 44.21±2.64 % and 39.34±0.66 mg/l/d at the end of the cultivation, respectively. Time-course transcript patterns of lipid biosynthesis genes i.e. acetyl coA carboxylase (accD) and diacylglycerol acyltransferase (dgat) showed that during late log phase of microalgae Chlorella sp.3 both the genes were significantly up regulated as compared to early log phase. Moreover, the transcript level of the dgat gene is two-fold higher than the accD gene. The results suggested that both the genes responded sensitively to the nitrogen limited conditions during the late log stage, which proposed their close relevance to lipid biosynthesis. Further, this transcriptome data will be useful for engineering microalgae species by targeting these genes for genetic modification to improve microalgal biofuel quality and production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofuel" title="biofuel">biofuel</a>, <a href="https://publications.waset.org/abstracts/search?q=gene" title=" gene"> gene</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid" title=" lipid"> lipid</a>, <a href="https://publications.waset.org/abstracts/search?q=microalgae" title=" microalgae"> microalgae</a> </p> <a href="https://publications.waset.org/abstracts/74923/time-course-lipid-accumulation-and-transcript-analyses-of-lipid-biosynthesis-gene-of-chlorella-sp3-under-nitrogen-limited-condition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74923.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">307</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3208</span> Upconversion Nanoparticle-Mediated Carbon Monoxide Prodrug Delivery System for Cancer Therapy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yaw%20Opoku-Damoah">Yaw Opoku-Damoah</a>, <a href="https://publications.waset.org/abstracts/search?q=Run%20Zhang"> Run Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Hang%20Thu%20Ta"> Hang Thu Ta</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhi%20Ping%20Xu"> Zhi Ping Xu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gas therapy is still at an early stage of research and development. Even though most gasotransmitters have proven their therapeutic potential, their handling, delivery, and controlled release have been extremely challenging. This research work employs a versatile nanosystem that is capable of delivering a gasotransmitter in the form of a photo-responsive carbon monoxide-releasing molecule (CORM) for targeted cancer therapy. The therapeutic action was mediated by upconversion nanoparticles (UCNPs) designed to transfer bio-friendly low energy near-infrared (NIR) light to ultraviolet (UV) light capable of triggering carbon monoxide (CO) from a water-soluble amphiphilic manganese carbonyl complex CORM incorporated into a carefully designed lipid drug delivery system. Herein, gaseous CO that plays a role as a gasotransmitter with cytotoxic and homeostatic properties was investigated to instigate cellular apoptosis. After successfully synthesizing the drug delivery system, the ability of the system to encapsulate and mediate the sustained release of CO after light excitation was demonstrated. CO fluorescence probe (COFP) was successfully employed to determine the in vitro drug release profile upon NIR light irradiation. The uptake of nanoparticles enhanced by folates and its receptor interaction was also studied for cellular uptake purposes. The anticancer potential of the final lipid nanoparticle Lipid/UCNPs/CORM/FA (LUCF) was also determined by cell viability assay. Intracellular CO release and a subsequent therapeutic action involving ROS production, mitochondrial damage, and CO production was also evaluated. In all, this current project aims to use in vitro studies to determine the potency and efficiency of a NIR-mediated CORM prodrug delivery system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=carbon%20monoxide-releasing%20molecule" title="carbon monoxide-releasing molecule">carbon monoxide-releasing molecule</a>, <a href="https://publications.waset.org/abstracts/search?q=upconversion%20nanoparticles" title=" upconversion nanoparticles"> upconversion nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=site-specific%20delivery" title=" site-specific delivery"> site-specific delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=amphiphilic%20manganese%20carbonyl%20complex" title=" amphiphilic manganese carbonyl complex"> amphiphilic manganese carbonyl complex</a>, <a href="https://publications.waset.org/abstracts/search?q=prodrug%20delivery%20system." title=" prodrug delivery system. "> prodrug delivery system. </a> </p> <a href="https://publications.waset.org/abstracts/124308/upconversion-nanoparticle-mediated-carbon-monoxide-prodrug-delivery-system-for-cancer-therapy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/124308.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">112</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=catanionic%20solid%20lipid%20nanoparticle&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=catanionic%20solid%20lipid%20nanoparticle&page=3">3</a></li> <li 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