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{"work":{"id":67578071,"created_at":"2022-01-07T21:25:51.560-08:00","from_world_paper_id":191102100,"updated_at":"2024-11-24T13:51:48.532-08:00","_data":{"publisher":"Kowsar Medical Institute","grobid_abstract":"Background: Based on many studies, otitis media with effusion (OME) is one of the major causes of childhood hearing loss, social malformation and medical costs. The pathogenesis still remains unclear, though it is known that this complication is closely related to bacterial infections. Alloiococcus otitidis, Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis are the most common bacterial pathogens isolated from middle ear effusions (MEEs). Objectives: Due to the prevalence of OME in children, we decided to investigate bacterial agents that cause diseases such as A. otitidis, H. influenzae, S. pneumonia and M. catarrhalis in these subjects. Patients and Methods: Forty-five children between one and 15 years of age were selected for this study. Seventy specimens were collected from MEE by myringotomy and inoculated in PBS buffer. Conventional culture and PCR methods were used for identification of bacterial agents. Results: The bacterial cultures in 8.6% of samples were positive by conventional culture, with A. otitidis, M. catarrhalis and S. pneumoniae present in 1.4%, 2.9% and 4.3% of samples, respectively. No H. influenzae was isolated. By the PCR method, A. otitidis was the most frequently isolated bacterium, found in 25.7% of samples, followed by S. pneumoniae, M. catarrhalis and H. influenzae, which were identified in 20%, 12% and 20% of samples, respectively. Overall, 55 out of 70 samples were positive by both the PCR and culture method. Conclusions: It can be concluded that A. otitidis was the major causative agent of MEE in children with OME. Therefore clinicians should be aware that bacterial infection plays an important role in the progression of acute otitis media to OME in children of our region.","publication_date":"2015,,","publication_name":"Jundishapur Journal of Microbiology","grobid_abstract_attachment_id":"78343484"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children With Otitis Media With Effusion","broadcastable":false,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [19858732]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 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class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />hamed goodarzi</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2015, Jundishapur Journal of Microbiology</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">5 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 67578071; const worksViewsPath = "/v0/works/views?subdomain_param=api&work_ids%5B%5D=67578071"; const 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class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="28383183" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/28383183/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients">Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in Iranian patients</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45029" href="https://tums.academia.edu/babaksaedi">Babak Saedi</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="53213336" href="https://yasujmedicalsciences.academia.edu/seyedsajjadkhoramrooz">seyed sajjad khoramrooz</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in Iranian patients","attachmentId":48718857,"attachmentType":"pdf","work_url":"https://www.academia.edu/28383183/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/28383183/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="28383181" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/28383181/Frequency_of_i_Alloicoccus_otitidis_i_i_Streptococcus_pneumoniae_i_i_Moraxella_catarrhalis_i_and_i_Haemophilus_influenzae_i_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients">Frequency of< i> Alloicoccus otitidis</i>,< i> Streptococcus pneumoniae</i>,< i> Moraxella catarrhalis</i> and< i> Haemophilus influenzae</i> in children with otitis media with effusion (OME) in Iranian patients</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45029" href="https://tums.academia.edu/babaksaedi">Babak Saedi</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="524053" href="https://tums.academia.edu/ERazmpa">Ebrahim Razmpa</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="53213336" href="https://yasujmedicalsciences.academia.edu/seyedsajjadkhoramrooz">seyed sajjad khoramrooz</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2012</p><p class="ds-related-work--abstract ds2-5-body-sm">OBJECTIVE: To determine the presence of common bacterial agents of otitis media with effusion (OME), together with investigation these agent in the adenoid tissue and antimicrobial susceptibility pattern of isolated bacteria in Iranian children with OME. METHODS: Polymerase chain reaction (PCR) and bacterial culture methods were used for detection and isolation of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in 63 middle ear fluid samples and 48 adenoid ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Frequency of\u003c i\u003e Alloicoccus otitidis\u003c/i\u003e,\u003c i\u003e Streptococcus pneumoniae\u003c/i\u003e,\u003c i\u003e Moraxella catarrhalis\u003c/i\u003e and\u003c i\u003e Haemophilus influenzae\u003c/i\u003e in children with otitis media with effusion (OME) in Iranian patients","attachmentId":48818707,"attachmentType":"pdf","work_url":"https://www.academia.edu/28383181/Frequency_of_i_Alloicoccus_otitidis_i_i_Streptococcus_pneumoniae_i_i_Moraxella_catarrhalis_i_and_i_Haemophilus_influenzae_i_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/28383181/Frequency_of_i_Alloicoccus_otitidis_i_i_Streptococcus_pneumoniae_i_i_Moraxella_catarrhalis_i_and_i_Haemophilus_influenzae_i_in_children_with_otitis_media_with_effusion_OME_in_Iranian_patients"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="889153" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/889153/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_">Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in …</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45029" href="https://tums.academia.edu/babaksaedi">Babak Saedi</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="524053" href="https://tums.academia.edu/ERazmpa">Ebrahim Razmpa</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in …","attachmentId":51179989,"attachmentType":"pdf","work_url":"https://www.academia.edu/889153/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/889153/Frequency_of_Alloicoccus_otitidis_Streptococcus_pneumoniae_Moraxella_catarrhalis_and_Haemophilus_influenzae_in_children_with_otitis_media_with_effusion_OME_in_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="33522874" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/33522874/Polymerase_chain_reaction_bacteriologic_detection_and_antibiogram_of_bacteria_isolated_from_otitis_media_with_effusion_in_children_shiraz_iran">Polymerase chain reaction, bacteriologic detection and antibiogram of bacteria isolated from otitis media with effusion in children, shiraz, iran</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="1847851" href="https://sums.academia.edu/mahmoodshishegar">mahmood shishegar</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Iranian journal of medical sciences, 2011</p><p class="ds-related-work--abstract ds2-5-body-sm">Otitis media with effusion is one of the leading causes of hearing loss in children. Effective treatment of effusion in the middle ear requires appropriate empirical treatment and characterization of responsible pathogens. Objective of the present study was to detect pathogens in clinical samples from patients with otitis media with effusion in our area and to determine the sensitivity profile of isolated organisms to commonly used antibiotics. Sixty three samples of middle ear effusion were aseptically obtained from 36 children, who had been treated up to at least two weeks before sampling. They were analyzed using standard bacteriological and multiplex polymerase chain reaction (PCR) assays. Antibiotic susceptibility tests were also performed. PCR analysis showed that DNA of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis were present in 60 (95.2%) of the samples. The culture-positive effusion for Streptococcus Pneumoniae, HaemophilusInfluenzae and Morax...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Polymerase chain reaction, bacteriologic detection and antibiogram of bacteria isolated from otitis media with effusion in children, shiraz, iran","attachmentId":53557681,"attachmentType":"pdf","work_url":"https://www.academia.edu/33522874/Polymerase_chain_reaction_bacteriologic_detection_and_antibiogram_of_bacteria_isolated_from_otitis_media_with_effusion_in_children_shiraz_iran","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/33522874/Polymerase_chain_reaction_bacteriologic_detection_and_antibiogram_of_bacteria_isolated_from_otitis_media_with_effusion_in_children_shiraz_iran"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="17519657" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/17519657/Identification_and_characterization_of_the_bacterial_etiology_of_clinically_problematic_acute_otitis_media_after_tympanocentesis_or_spontaneous_otorrhea_in_German_children">Identification and characterization of the bacterial etiology of clinically problematic acute otitis media after tympanocentesis or spontaneous otorrhea in German children</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37287925" href="https://gsk.academia.edu/CinziaMarano">Cinzia Marano</a></div><p class="ds-related-work--metadata ds2-5-body-xs">BMC infectious diseases, 2012</p><p class="ds-related-work--abstract ds2-5-body-sm">Acute Otitis Media (AOM) is an important and common disease of childhood. Bacteria isolated from cases of clinically problematic AOM in German children were identified and characterized. In a prospective non-interventional study in German children between 3 months and less than 60 months of age with Ear, Nose and Throat Specialist -confirmed AOM, middle ear fluid was obtained by tympanocentesis (when clinically indicated) or by careful sampling of otorrhea through/at an existing perforation. In 100 children with severe AOM, Haemophilus influenzae was identified in 21% (18/21, 85.7% were non-typeable [NTHi]), Streptococcus pneumoniae in 10%, S. pyogenes in 13% and Moraxella catarrhalis in 1%. H. influenzae was the most frequently identified pathogen in children from 12 months of age. H. influenzae and S. pneumoniae were equally prevalent in children aged 3-11 months, but S. pyogenes was most frequently isolated in this age group. NTHi AOM disease appeared prevalent in all ages. NTHi,...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Identification and characterization of the bacterial etiology of clinically problematic acute otitis media after tympanocentesis or spontaneous otorrhea in German children","attachmentId":42252026,"attachmentType":"pdf","work_url":"https://www.academia.edu/17519657/Identification_and_characterization_of_the_bacterial_etiology_of_clinically_problematic_acute_otitis_media_after_tympanocentesis_or_spontaneous_otorrhea_in_German_children","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17519657/Identification_and_characterization_of_the_bacterial_etiology_of_clinically_problematic_acute_otitis_media_after_tympanocentesis_or_spontaneous_otorrhea_in_German_children"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="80952050" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media">Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="67299631" href="https://independent.academia.edu/RavinderKaur87">Ravinder Kaur</a></div><p class="ds-related-work--metadata ds2-5-body-xs">The Pediatric infectious disease journal, 2010</p><p class="ds-related-work--abstract ds2-5-body-sm">The 3 most commonly encountered bacteria in acute otitis media (AOM) are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Conventional culture methods detect these pathogens in only 60% to 70% of cases of AOM. Alloiococcus otitidis, another potential pathogen, has often been ignored. Tympanocentesis was performed in 97 children with AOM presenting with a bulging tympanic membrane (TM) producing 170 middle ear fluids (MEFs). S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis were isolated in 21%, 32%, 8%, and 0% of MEFs, respectively; no otopathogen was isolated in 29% of MEFs. In nasopharyngeal cultures at the time of AOM diagnosis, 34%, 36%, 17%, and 0% and in oropharyngeal cultures, 7%, 31%, 11%, and 0% grew S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis, respectively. No otopathogen was isolated in 23% of nasopharyngeal and 20% of oropharyngeal cultures. Multiplex polymerase chain reaction (PCR) was used to detect DNA of the 4...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Simultaneous assay for four bacterial species including Alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media","attachmentId":87162382,"attachmentType":"pdf","work_url":"https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/80952050/Simultaneous_assay_for_four_bacterial_species_including_Alloiococcus_otitidis_using_multiplex_PCR_in_children_with_culture_negative_acute_otitis_media"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="24572931" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/24572931/Two_step_PCR_based_assay_for_identification_of_bacterial_etiology_of_otitis_media_with_effusion_in_infected_Lebanese_children">Two-step PCR-based assay for identification of bacterial etiology of otitis media with effusion in infected Lebanese children</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="47367128" href="https://independent.academia.edu/FayadMichel">Michel Fayad</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of clinical microbiology, 1998</p><p class="ds-related-work--abstract ds2-5-body-sm">We developed and evaluated a two-step PCR-based assay with universal primers and genus- or species-specific primers for the detection of the most prevalent bacterial etiologies of otitis media with effusion (OME) in children from Lebanese hospitals. These etiologies included Haemophilus, Streptococcus, and Moraxella (Branhamella) catarrhalis, which were detected in middle-ear effusion (MEE) samples taken from children with OME. A total of 47 MEE samples were aspirated from 36 patients during insertion of a tympanostomy tube performed particularly for OME. The duration of effusion in all patients was &gt; or =2 months. DNA was extracted from MEE samples, and PCR was initially done with DNA extracts by using the universal primers RW01 and DG74, which flank an approximately 370-bp fragment found in the 16S rRNA gene of all bacterial species. For the identification of specific bacteria, we used in three separate reaction mixtures the following genus- or species-specific primers: (i) a H...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Two-step PCR-based assay for identification of bacterial etiology of otitis media with effusion in infected Lebanese children","attachmentId":44901779,"attachmentType":"pdf","work_url":"https://www.academia.edu/24572931/Two_step_PCR_based_assay_for_identification_of_bacterial_etiology_of_otitis_media_with_effusion_in_infected_Lebanese_children","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/24572931/Two_step_PCR_based_assay_for_identification_of_bacterial_etiology_of_otitis_media_with_effusion_in_infected_Lebanese_children"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="10942648" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/10942648/Concurrent_Assay_for_Four_Bacterial_Species_Including_Alloiococcus_Otitidis_in_Middle_Ear_Nasopharynx_and_Tonsils_of_Children_with_Otitis_Media_with_Effusion_A_Preliminary_Report">Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="26524495" href="https://independent.academia.edu/EmineAyd%C4%B1n4">Emine Aydın</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinical and Experimental Otorhinolaryngology, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report","attachmentId":47009831,"attachmentType":"pdf","work_url":"https://www.academia.edu/10942648/Concurrent_Assay_for_Four_Bacterial_Species_Including_Alloiococcus_Otitidis_in_Middle_Ear_Nasopharynx_and_Tonsils_of_Children_with_Otitis_Media_with_Effusion_A_Preliminary_Report","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/10942648/Concurrent_Assay_for_Four_Bacterial_Species_Including_Alloiococcus_Otitidis_in_Middle_Ear_Nasopharynx_and_Tonsils_of_Children_with_Otitis_Media_with_Effusion_A_Preliminary_Report"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="62925795" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/62925795/Acute_otitis_media_in_children_of_Ismailia_city_a_bacteriological_study_">Acute otitis media in children of Ismailia city ( a bacteriological study)</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="56444662" href="https://independent.academia.edu/AmiraGamal11">Amira Gamal</a></div><p class="ds-related-work--metadata ds2-5-body-xs">The Egyptian Journal of Otolaryngology, 2013</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Acute otitis media in children of Ismailia city ( a bacteriological study)","attachmentId":75531947,"attachmentType":"pdf","work_url":"https://www.academia.edu/62925795/Acute_otitis_media_in_children_of_Ismailia_city_a_bacteriological_study_","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/62925795/Acute_otitis_media_in_children_of_Ismailia_city_a_bacteriological_study_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="23308796" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/23308796/Lack_of_Chlamydophila_pneumoniae_and_predominance_of_Alloiococcus_otitidis_in_middle_ear_fluids_of_children_with_otitis_media_with_effusion">Lack of Chlamydophila pneumoniae and predominance of Alloiococcus otitidis in middle ear fluids of children with otitis media with effusion</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45077379" href="https://independent.academia.edu/EnderInci">Ender Inci</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Auris Nasus Larynx, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Lack of Chlamydophila pneumoniae and predominance of Alloiococcus otitidis in middle ear fluids of children with otitis media with effusion","attachmentId":43771133,"attachmentType":"pdf","work_url":"https://www.academia.edu/23308796/Lack_of_Chlamydophila_pneumoniae_and_predominance_of_Alloiococcus_otitidis_in_middle_ear_fluids_of_children_with_otitis_media_with_effusion","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link 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You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="52291331" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/52291331/Bacterial_etiology_and_serotypes_of_acute_otitis_media_in_Mexican_children">Bacterial etiology and serotypes of acute otitis media in Mexican children</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="187588219" href="https://independent.academia.edu/YolandaCervantes15">Yolanda Cervantes</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Vaccine, 2011</p><div 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translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="1" data-entity-id="15238147" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/15238147/Association_of_Clinical_Signs_and_Symptoms_with_Bacterial_Findings_in_Acute_Otitis_Media">Association of Clinical Signs and Symptoms with Bacterial Findings in Acute Otitis Media</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34309565" href="https://independent.academia.edu/ArtoPalmu">Arto Palmu</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinical Infectious Diseases, 2004</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Association of Clinical Signs and Symptoms with Bacterial Findings in Acute Otitis Media","attachmentId":43408931,"attachmentType":"pdf","work_url":"https://www.academia.edu/15238147/Association_of_Clinical_Signs_and_Symptoms_with_Bacterial_Findings_in_Acute_Otitis_Media","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/15238147/Association_of_Clinical_Signs_and_Symptoms_with_Bacterial_Findings_in_Acute_Otitis_Media"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="2" data-entity-id="22569292" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/22569292/Otitis_media_with_effusion_in_Lebanese_children_prevalence_and_pathogen_susceptibility">Otitis media with effusion in Lebanese children: prevalence and pathogen susceptibility</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="44118441" href="https://independent.academia.edu/NabilMoukarzel">Nabil Moukarzel</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="44139143" href="https://independent.academia.edu/HassanHaidar2">Hassan Haidar</a></div><p class="ds-related-work--metadata ds2-5-body-xs">The Journal of Laryngology & Otology, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link 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