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assay</title> <meta name="description" content="Search results for: antitumor assay"> <meta name="keywords" content="antitumor assay"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="antitumor assay"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 1233</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: antitumor assay</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1233</span> Design, Synthesis and in-vitro Antitumor Evaluation of Some Novel Substituted Quinazoline Derivatives</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adel%20S.%20El-Azab">Adel S. El-Azab</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20A.%20M.%20Abdel-Aziz"> Alaa A. M. Abdel-Aziz</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20A.%20Al-Suwaidan"> Ibrahim A. Al-Suwaidan</a>, <a href="https://publications.waset.org/abstracts/search?q=Amer%20M.%20Alanazi"> Amer M. Alanazi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A novel series of 2,3,6-trisubstitute quinazolinone were designed, synthesized, and evaluated for their in-vitro antitumor activity. 3 (Benzylideneamino)-6-chloro-2-p-tolylquinazolin-4(3H)-One, 2-[(4-oxo-3-phenethyl-3,4-dihydroquinazolin-2-yl)thio]-N-(3,4;5-trimethoxyphenyl) acetamide and 3-(3-benzyl-6-methyl-4-oxo-3, 4-dihydroquinazolin-2-ylthio)-N-(3,4,5-trimethoxyphenyl) propanamide have shown amazing broad spectrum antitumor activity with mean GI50; 15.8, 3.16, and 7.4 μM respectively compared to known Quinazoline Derivatives antitumor drug 5-FU mean GI50=22.6 μM. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=quinazoline%20derivatives" title="quinazoline derivatives">quinazoline derivatives</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20antitumor" title=" in vitro antitumor"> in vitro antitumor</a>, <a href="https://publications.waset.org/abstracts/search?q=synthesis" title=" synthesis"> synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=5-FU" title=" 5-FU"> 5-FU</a>, <a href="https://publications.waset.org/abstracts/search?q=NCI" title=" NCI"> NCI</a> </p> <a href="https://publications.waset.org/abstracts/22501/design-synthesis-and-in-vitro-antitumor-evaluation-of-some-novel-substituted-quinazoline-derivatives" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22501.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">544</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1232</span> Metabolites of Polygonum L. Plants Having Antitumor Properties</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dmitriy%20Yu.%20Korulkin">Dmitriy Yu. Korulkin</a>, <a href="https://publications.waset.org/abstracts/search?q=Raissa%20A.%20Muzychkina"> Raissa A. Muzychkina</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The article represents the results of research of antitumor activity of different structural types of plant flavonoids extracted by authors from Polygonum L. plants in commercial reserves at the territory of the Republic of Kazakhstan. For the first time ever the results comparative research of antitumor activity of plant flavonoids of different structural groups and their synthetic derivatives have been represented. The results of determination of toxicity of flavonoids in single parenteral infusion conditions have been represented. Experimental substantiation of possible mechanisms of antiproliferative and cytotoxic action of flavonoids has been suggested. The perspectives of usage of plant flavonoids as medications and creation of effective dosage forms of antitumor medicines on their basis have been substantiated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antitumor%20activity" title="antitumor activity">antitumor activity</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=flavonoids" title=" flavonoids"> flavonoids</a>, <a href="https://publications.waset.org/abstracts/search?q=Polygonum%20L." title=" Polygonum L."> Polygonum L.</a>, <a href="https://publications.waset.org/abstracts/search?q=secondary%20metabolites" title=" secondary metabolites"> secondary metabolites</a> </p> <a href="https://publications.waset.org/abstracts/28922/metabolites-of-polygonum-l-plants-having-antitumor-properties" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28922.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">260</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1231</span> Physicochemical and Functional significance of Two Lychee (Litchi chinensis Sonn.) Cultivars Gola and Surakhi from Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Naila%20Safdar">Naila Safdar</a>, <a href="https://publications.waset.org/abstracts/search?q=Faria%20Riasat"> Faria Riasat</a>, <a href="https://publications.waset.org/abstracts/search?q=Azra%20Yasmin"> Azra Yasmin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lychee is an emerging fruit crop in Pakistan. Two famous cultivars of lychee, Gola and Surakhi, were collected from Khanpur Orchard, Pakistan and their whole fruit (including peel, pulp and seed) was investigated for pomological features and therapeutic activities. Both cultivars differ in shape and size with Gola having large size (3.27cm length, 2.36cm width) and more flesh to seed ratio (8.65g). FTIR spectroscopy and phytochemical tests confirmed presence of different bioactive compounds like phenol, flavonoids, quinones, anthraquinones, tannins, glycosides, and alkaloids, in both lychee fruits. Atomic absorption spectroscopy indicated an increased amount of potassium, magnesium, sodium, iron, and calcium in Gola and Surakhi fruits. Small amount of trace metals, zinc and copper, were also detected in lychee fruit, while heavy metals lead, mercury, and nickel were absent. These two lychee cultivars were also screened for antitumor activity by Potato disc assay with maximum antitumor activity shown by aqueous extract of Surakhi seed (77%) followed by aqueous extract of Gola pulp (74%). Antimicrobial activity of fruit parts was checked by agar well diffusion method against six bacterial strains Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis, Bacillus sp. MB083, and Bacillus sp. MB141. Highest antimicrobial activity was shown by methanolic extract of Gola pulp (27mm ± 0.70) and seed (19.5mm ± 0.712) against Enterococcus faecalis. DPPH scavenging assay revealed highest antioxidant activity by aqueous extract of Gola peel (98.10%) followed by n-hexane extract of Surakhi peel (97.73%). Results obtained by reducing power assay also corroborated with the results of DPPH scavenging activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20evaluation" title="antimicrobial evaluation">antimicrobial evaluation</a>, <a href="https://publications.waset.org/abstracts/search?q=antitumor%20assay" title=" antitumor assay"> antitumor assay</a>, <a href="https://publications.waset.org/abstracts/search?q=gola" title=" gola"> gola</a>, <a href="https://publications.waset.org/abstracts/search?q=phytoconstituents" title=" phytoconstituents"> phytoconstituents</a>, <a href="https://publications.waset.org/abstracts/search?q=reactive%20oxygen%20species" title=" reactive oxygen species"> reactive oxygen species</a>, <a href="https://publications.waset.org/abstracts/search?q=Surakhi" title=" Surakhi"> Surakhi</a> </p> <a href="https://publications.waset.org/abstracts/28614/physicochemical-and-functional-significance-of-two-lychee-litchi-chinensis-sonn-cultivars-gola-and-surakhi-from-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28614.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">408</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1230</span> Synthesis and Anti-Cancer Evaluation of Uranyle Complexes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdol-Hassan%20Doulah">Abdol-Hassan Doulah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this research, some of the inorganic complexes of uranyl with N- donor ligands were synthesized. Complexes were characteriezed by FT-IR and UV spectra, ¹HNMR, ¹³CNMR and some physical properties. The uranyl unit (UO2) is composed of a center of uranium atom with the charge (+6) and two oxygen atom by forming two U=O double bonds. The structure is linear (O=U=O, 180) and usually stable. So other ligands often coordinate to the U atom in the plane perpendicularly to the O=U=O axis. The antitumor activity of some of ligand and their complexes against a panel of human tumor cell lines (HT29: Haman colon adenocarcinoma cell line T47D: human breast adenocarcinoma cell line) were determined by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay. These data suggest that some of these compounds provide good models for the further design of potent antitumor compounds. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=inorganic" title="inorganic">inorganic</a>, <a href="https://publications.waset.org/abstracts/search?q=uranyl%20complex-donor%20ligands" title=" uranyl complex-donor ligands"> uranyl complex-donor ligands</a>, <a href="https://publications.waset.org/abstracts/search?q=Schiff%20bases" title=" Schiff bases"> Schiff bases</a>, <a href="https://publications.waset.org/abstracts/search?q=anticancer%20activity" title=" anticancer activity"> anticancer activity</a> </p> <a href="https://publications.waset.org/abstracts/23527/synthesis-and-anti-cancer-evaluation-of-uranyle-complexes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23527.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">454</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1229</span> Discovery, Design and Synthesis of Some Novel Antitumor 1,2,4-Triazine Derivatives as C-Met Kinase Inhibitors</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20M.%20Labouta">Ibrahim M. Labouta</a>, <a href="https://publications.waset.org/abstracts/search?q=Marwa%20H.%20El-Wakil"> Marwa H. El-Wakil</a>, <a href="https://publications.waset.org/abstracts/search?q=Hayam%20M.%20Ashour"> Hayam M. Ashour</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20M.%20Hassan"> Ahmed M. Hassan</a>, <a href="https://publications.waset.org/abstracts/search?q=Manal%20N.%20Saudi"> Manal N. Saudi </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The receptor tyrosine kinase c-Met is an attractive target for therapeutic treatment of cancers nowadays. Among the wide variety of heterocycles that have been explored for developing c-Met kinase inhibitors, the 1,2,4-triazines have been rarely investigated, although they are well known in the literature to possess antitumor activities. Herein we describe the design and synthesis of a novel series of 1,2,4-triazine derivatives possessing N-acylarylhydrazone moiety and another series combining the 1,2,4-triazine scaffold to the well-known anticancer drug 6-MP in order to explore their “double-drug” effect. The synthesized compounds were evaluated for their in vitro antitumor activity against three c-Met addicted cancer cell lines (A549, HT-29 and MKN-45). Most compounds showed moderate to excellent antiproliferative activity and four compounds showed potent inhibitory activity more than the reference drug Foretinib against one or more cancer cell lines. The obtained results revealed that the potent compounds are highly selective to A549 (lung adenocarcinoma) cancer cell line. The c-Met kinase inhibitory activity of the potent derivatives is still under investigation. The present study clearly demonstrates that the 1,2,4-triazine core ring exhibits promising antitumor activity with potential c-Met kinase inhibitory activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=1" title="1">1</a>, <a href="https://publications.waset.org/abstracts/search?q=2" title="2">2</a>, <a href="https://publications.waset.org/abstracts/search?q=4-triazine" title="4-triazine">4-triazine</a>, <a href="https://publications.waset.org/abstracts/search?q=antitumor" title=" antitumor"> antitumor</a>, <a href="https://publications.waset.org/abstracts/search?q=c-Met%20inhibitor" title=" c-Met inhibitor"> c-Met inhibitor</a>, <a href="https://publications.waset.org/abstracts/search?q=double-drug" title=" double-drug"> double-drug</a> </p> <a href="https://publications.waset.org/abstracts/47612/discovery-design-and-synthesis-of-some-novel-antitumor-124-triazine-derivatives-as-c-met-kinase-inhibitors" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47612.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">339</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1228</span> Mentha crispa Essential Oil and Rotundifolone Analogues: Cytotoxic Effect on Glioblastoma</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dami%C3%A3o%20Sousa">Damião Sousa</a>, <a href="https://publications.waset.org/abstracts/search?q=Hasan%20Turkez"> Hasan Turkez</a>, <a href="https://publications.waset.org/abstracts/search?q=Ozlem%20Tozlu"> Ozlem Tozlu</a>, <a href="https://publications.waset.org/abstracts/search?q=Tamires%20Lima"> Tamires Lima</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Glioblastoma (GBM) is an aggressive cancer from the brain and with high prevalence and significant morbimortality. Therefore, it is necessary to investigate new therapeutic options against this pathology. Thus, the purpose of this study was to evaluate the antitumor activity from Mentha crispa essential oil (MCEO), its major constituent rotundifolone (ROT) and a series of six analogues on human U87MG glioblastoma cell line. The antitumor effects of the compounds on human U87MG-GBM cell line were assessed using in vitro cell viability assays. In addition, biosafety tests were performed on cultured human blood cells. The data show that MCEO, 1,2-perillaldehyde epoxide (EPER1) and perillaldehyde (PALD) were the most cytotoxic compounds against the U87MG cells, with IC50 values of 16.263, 15.087 and 14.888 μg/mL, respectively. The treatment with MCEO, EPER1 and PALD did not lead to damage in blood cells. These chemical analogues may be useful as prototypes for development of novel antitumor drugs due to their promising activities and toxicological safety. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antitumor%20activity" title="antitumor activity">antitumor activity</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer" title=" cancer"> cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20products" title=" natural products"> natural products</a>, <a href="https://publications.waset.org/abstracts/search?q=terpenes" title=" terpenes"> terpenes</a> </p> <a href="https://publications.waset.org/abstracts/90514/mentha-crispa-essential-oil-and-rotundifolone-analogues-cytotoxic-effect-on-glioblastoma" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90514.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">148</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1227</span> White-Rot Fungi Phellinus as a Source of Antioxidant and Antitumor Agents</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yogesh%20Dalvi">Yogesh Dalvi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ruby%20Varghese"> Ruby Varghese</a>, <a href="https://publications.waset.org/abstracts/search?q=Nibu%20Varghese"> Nibu Varghese</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20K.%20Krishnan%20Nair"> C. K. Krishnan Nair</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: The Genus Phellinus, locally known as Phansomba is a well-known traditional folk medicine. Especially, in Western Ghats of India, many tribes use several species of Phellinus for various ailments related to teeth, throat, tongue, stomach and even wound healing. It is one of the few mushrooms which play a pivotal role in Ayurvedic Dravyaguna. Aim: The present study focuses on to investigate phytochemical analysis, antioxidant, and antitumor (in vitro and in vivo) potential of Phellinus robinae from South India, Kerala Material and Methods: The present study explores the following: 1. Phellinus samples were collected from Ranni, Pathanamthitta district of Kerala state, India from Artocarpus heterophyllus Lam. and species were identified using rDNA region. 2. The fruiting body was shadow dried, powdered and extracted with 50% alcohol using water bath at 60°C which was further condensed by rotary evaporator and lyophilized at minus 40°C temperature. 3. Secondary metabolites were analyzed by using various phytochemical screening assay (Hager’s Test, Wagner’s Test, Sodium hydroxide Test, Lead acetate Test, Ferric chloride Test, Folin-ciocalteu Test, Foaming Test, Benedict’s test, Fehling’s Test and Lowry’s Test). 4. Antioxidant and free radical scavenging activity were analyzed by DPPH, FRAP and Iron chelating assay. 5. The antitumor potential of Water alcohol extract of Phellinus (PAWE) is evaluated through In vitro condition by Trypan blue dye exclusion method in DLA cell line and In vivo by murine model. Result and Discussion: Preliminary phytochemical screening by various biochemical tests revealed presence of a variety of active secondary molecules like alkaloids, flavanoids, saponins, carbohydrate, protein and phenol. In DPPH and FRAP assay PAWE showed significantly higher antioxidant activity as compared to standard Ascorbic acid. While, in Iron chelating assay, PAWE exhibits similar antioxidant activity that of Butylated Hydroxytoluene (BHT) as standard. Further, in the in vitro study, PAWE showed significant inhibition on DLA cell proliferation in dose dependent manner and showed no toxicity on mice splenocytes, when compared to standard chemotherapy drug doxorubicin. In vivo study, oral administration of PAWE showed dose dependent tumor regression in mice and also raised the immunogenicity by restoring levels of antioxidant enzymes in liver and kidney tissue. In both in vitro and in vivo gene expression studies PAWE up-regulates pro-apoptotic genes (Bax, Caspases 3, 8 and 9) and down- regulates anti-apoptotic genes (Bcl2). PAWE also down regulates inflammatory gene (Cox-2) and angiogenic gene (VEGF). Conclusion: Preliminary phytochemical screening revealed that PAWE contains various secondary metabolites which contribute to its antioxidant and free radical scavenging property as evaluated by DPPH, FRAP and Iron chelating assay. PAWE exhibits anti-proliferative activity by the induction of apoptosis through a signaling cascade of death receptor-mediated extrinsic (Caspase8 and Tnf-α), as well as mitochondria-mediated intrinsic (caspase9) and caspase pathways (Caspase3, 8 and 9) and also by regressing angiogenic factor (VEGF) without any inflammation or adverse side effects. Hence, PAWE serve as a potential antioxidant and antitumor agent. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title="antioxidant">antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=antitumor" title=" antitumor"> antitumor</a>, <a href="https://publications.waset.org/abstracts/search?q=Dalton%20lymphoma%20ascites%20%28DLA%29" title=" Dalton lymphoma ascites (DLA)"> Dalton lymphoma ascites (DLA)</a>, <a href="https://publications.waset.org/abstracts/search?q=fungi" title=" fungi"> fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=Phellinus%20robinae" title=" Phellinus robinae"> Phellinus robinae</a> </p> <a href="https://publications.waset.org/abstracts/68802/white-rot-fungi-phellinus-as-a-source-of-antioxidant-and-antitumor-agents" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68802.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">305</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1226</span> Ameliorating Effects of Rosemary and Costus on Blood-Associated Toxicity in Ehrlich-Bearing Mice Treated with Cisplatin</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yousry%20El-Sayed%20Elbolkiny">Yousry El-Sayed Elbolkiny</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Labib%20%20Salem"> Mohamed Labib Salem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Rosemary (ROLE) and costus (SLRE) have been established to show antioxidant effects. Aim: This study aimed to evaluate the ameliorating effects of ROLE and SLRE on the side effects induced by cisplatin (CIS) in tumor-bearing mice. Materials and Methods: Extracts of ROLE and SLRE were examined for their phytochemical activities. To evaluate their anti-tumor effects, mice were inoculated intraperitoneally (i.p.) with 2.5x105 Ehrlich ascites carcinoma (EAC) and then treated i.p. with CIS at days 3-7 and with ROLE (dose) or SLRE (dose) at days 3-14. Mice were sacrificed on day 14 for CBC and T-cell analyses. Results: Phytochemical analysis revealed that both ROLE and SLRE showed similar antioxidant activities. Treatment of EAC-bearing mice with CIS-induced antitumor efficacy of about 90%. Treatment with CIS in combination with ROLE or SLRE did not further enhance the antitumor activity of CIS. However, co-administration of ROLE or SLRE with CIS significantly increased the antitumor efficacy of CIS. Flow cytometric analysis showed that the numbers of CD4+ and CD8+ T cells were decreased in EAC-bearing mice after treatment with CIS. Treatment with both ROLE and SLRE improved the number of these cells. Conclusion: Combinatorial treatment with rosemary and costus can enhance the antitumor activity of CIS <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CBC" title="CBC">CBC</a>, <a href="https://publications.waset.org/abstracts/search?q=cisplantin" title=" cisplantin"> cisplantin</a>, <a href="https://publications.waset.org/abstracts/search?q=costus" title=" costus"> costus</a>, <a href="https://publications.waset.org/abstracts/search?q=rosemary" title=" rosemary"> rosemary</a> </p> <a href="https://publications.waset.org/abstracts/185377/ameliorating-effects-of-rosemary-and-costus-on-blood-associated-toxicity-in-ehrlich-bearing-mice-treated-with-cisplatin" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185377.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">49</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1225</span> Anticancer Study of Copper and Zinc Complexes with Doxorubicin</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Grzegorz%20Swiderski">Grzegorz Swiderski</a>, <a href="https://publications.waset.org/abstracts/search?q=Agata%20Jablonska-Trypuc"> Agata Jablonska-Trypuc</a>, <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Popow"> Natalia Popow</a>, <a href="https://publications.waset.org/abstracts/search?q=Renata%20Swislocka"> Renata Swislocka</a>, <a href="https://publications.waset.org/abstracts/search?q=Wlodzimierz%20Lewandowski"> Wlodzimierz Lewandowski</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Doxorubicin belongs to the group of anthracycline antitumor antibiotics. Because of the wide spectrum of actions, it is one of the most widely used anthracycline antibiotics, including the treatment of breast, ovary, bladder, lung cancers as well as neuroblastoma, lymphoma, leukemia and myeloid leukemia. Antitumor activity of doxorubicin is based on the same mechanisms as for most anthracyclines. Like the metal ions affect the nucleic acids on many biological processes, so the environment of the metal chelates of antibiotics can have a significant effect on the pharmacological properties of drugs. Complexation of anthracyclines with metal ions may contribute to the production of less toxic compounds. In the framework of this study, the composition of complexes obtained in aqueous solutions of doxorubicin with metal ions (Cu2+ and Zn2+). Complexation was analyzed by spectrophotometric titration in aqueous solution at pH 7.0. The pH was adjusted with 0.02M Tris-HCl buffer. The composition of the complexes found was Cu: doxorubicin (1: 2) and a Zn: doxorubicin (1: 1). The effect of Dox, Dox-Cu and Dox-Zn was examined in MCF-7 breast cancer cell line, which were obtained from American Type Culture Collection (ATCC). The compounds were added to the cultured cells for a final concentration in the range of 0,01µM to 0,5µM. The number of MCF-7 cells with division into living and dead, was determined by direct counts of cells with the use of trypan blue dye using LUNA Logos Biosystems cell counter. ApoTox-Glo Triplex Assay (Promega, Madison, Wisconsin, USA) was used according to the manufacturer’s instructions to measure the MCF-7 cells’ viability, cytotoxicity and apoptosis. We observed a decrease in cells proliferation in a dose-dependent manner. An increase in cytotoxicity and decrease in viability in the ApoTox Triplex assay was also showed for all tested compounds. Apoptosis, showed as caspase 3/7 activation, was observed only in Dox treatment. In Dox-Zn and Dox-Cu caspase 3/7 activation was not observed. This work was financially supported by National Science Centre, Poland, under the research project number 2014/13/B/NZ7/02 352. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anticancer%20properties" title="anticancer properties">anticancer properties</a>, <a href="https://publications.waset.org/abstracts/search?q=anthracycline%20antibiotic" title=" anthracycline antibiotic"> anthracycline antibiotic</a>, <a href="https://publications.waset.org/abstracts/search?q=doxorubicine" title=" doxorubicine"> doxorubicine</a>, <a href="https://publications.waset.org/abstracts/search?q=metal%20complexes" title=" metal complexes"> metal complexes</a> </p> <a href="https://publications.waset.org/abstracts/63652/anticancer-study-of-copper-and-zinc-complexes-with-doxorubicin" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63652.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1224</span> Performance of the Aptima® HIV-1 Quant Dx Assay on the Panther System </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Siobhan%20O%E2%80%99Shea">Siobhan O’Shea</a>, <a href="https://publications.waset.org/abstracts/search?q=Sangeetha%20Vijaysri%20Nair"> Sangeetha Vijaysri Nair</a>, <a href="https://publications.waset.org/abstracts/search?q=Hee%20Cheol%20Kim"> Hee Cheol Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Charles%20Thomas%20Nugent"> Charles Thomas Nugent</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheuk%20Yan%20William%20Tong"> Cheuk Yan William Tong</a>, <a href="https://publications.waset.org/abstracts/search?q=Sam%20Douthwaite"> Sam Douthwaite</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrew%20Worlock"> Andrew Worlock</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Aptima® HIV-1 Quant Dx Assay is a fully automated assay on the Panther system. It is based on Transcription-Mediated Amplification and real time detection technologies. This assay is intended for monitoring HIV-1 viral load in plasma specimens and for the detection of HIV-1 in plasma and serum specimens. Nine-hundred and seventy nine specimens selected at random from routine testing at St Thomas’ Hospital, London were anonymised and used to compare the performance of the Aptima HIV-1 Quant Dx assay and Roche COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, v2.0. Two-hundred and thirty four specimens gave quantitative HIV-1 viral load results in both assays. The quantitative results reported by the Aptima Assay were comparable those reported by the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, v2.0 with a linear regression slope of 1.04 and an intercept on -0.097. The Aptima assay detected HIV-1 in more samples than the Roche assay. This was not due to lack of specificity of the Aptima assay because this assay gave 99.83% specificity on testing plasma specimens from 600 HIV-1 negative individuals. To understand the reason for this higher detection rate a side-by-side comparison of low level panels made from the HIV-1 3rd international standard (NIBSC10/152) and clinical samples of various subtypes were tested in both assays. The Aptima assay was more sensitive than the Roche assay. The good sensitivity, specificity and agreement with other commercial assays make the HIV-1 Quant Dx Assay appropriate for both viral load monitoring and detection of HIV-1 infections. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=HIV%20viral%20load" title="HIV viral load">HIV viral load</a>, <a href="https://publications.waset.org/abstracts/search?q=Aptima" title=" Aptima"> Aptima</a>, <a href="https://publications.waset.org/abstracts/search?q=Roche" title=" Roche"> Roche</a>, <a href="https://publications.waset.org/abstracts/search?q=Panther%20system" title=" Panther system"> Panther system</a> </p> <a href="https://publications.waset.org/abstracts/21163/performance-of-the-aptima-hiv-1-quant-dx-assay-on-the-panther-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21163.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">375</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1223</span> ICAM-2, A Protein of Antitumor Immune Response in Mekong Giant Catfish (Pangasianodon gigas)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jiraporn%20Rojtinnakorn">Jiraporn Rojtinnakorn</a> </p> <p class="card-text"><strong>Abstract:</strong></p> ICAM-2 (intercellular adhesion molecule 2) or CD102 (Cluster of Differentiation 102) is type I trans-membrane glycoproteins, composing 2-9 immunoglobulin-like C2-type domains. ICAM-2 plays the particular role in immune response and cell surveillance. It is concerned in innate and specific immunity, cell survival signal, apoptosis, and anticancer. EST clone of ICAM-2, from P. gigas blood cell EST libraries, showed high identity to human ICAM-2 (92%) with conserve region of ICAM N-terminal domain and part of Ig superfamily. Gene and protein of ICAM-2 has been founded in mammals. This is the first report of ICAM-2 in fish. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ICAM-2" title="ICAM-2">ICAM-2</a>, <a href="https://publications.waset.org/abstracts/search?q=CD102" title=" CD102"> CD102</a>, <a href="https://publications.waset.org/abstracts/search?q=Pangasianodon%20gigas" title=" Pangasianodon gigas"> Pangasianodon gigas</a>, <a href="https://publications.waset.org/abstracts/search?q=antitumor" title=" antitumor"> antitumor</a> </p> <a href="https://publications.waset.org/abstracts/5214/icam-2-a-protein-of-antitumor-immune-response-in-mekong-giant-catfish-pangasianodon-gigas" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5214.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">227</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1222</span> Synthesis and Biological Activity Evaluation of U Complexes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Kazem%20Mohammadi">Mohammad Kazem Mohammadi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The use of anticancer agents forms an important part of the treatment of cancer of various types. Uranyl Complexes with DPHMP ligand have been used for the prevention and treatment of cancers. U(IV) metal complexes prepared by reaction of uranyl salt UO2 (NO3)2.6H2O with DPHMP in dry acetonitrile. Characterization of the ligand and its complexes was made by microanalyses, FT-IR, 1H NMR, 13C NMR and UV–Visible spectroscopy. These new complex showed excellent antitumor activity against two kinds of cancer cells that that are HT29:Haman colon adenocarcinoma cell line and T47D:human breast adenocarcinoma cell line. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=uranyl%20complexes" title="uranyl complexes">uranyl complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=DPHMP%20ligand" title=" DPHMP ligand"> DPHMP ligand</a>, <a href="https://publications.waset.org/abstracts/search?q=antitumor%20activity" title=" antitumor activity"> antitumor activity</a>, <a href="https://publications.waset.org/abstracts/search?q=HT29" title=" HT29"> HT29</a>, <a href="https://publications.waset.org/abstracts/search?q=T47D" title=" T47D"> T47D</a> </p> <a href="https://publications.waset.org/abstracts/23976/synthesis-and-biological-activity-evaluation-of-u-complexes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23976.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">470</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1221</span> A pH-Activatable Nanoparticle Self-Assembly Triggered by 7-Amino Actinomycin D Demonstrating Superior Tumor Fluorescence Imaging and Anticancer Performance</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Han%20Xiao">Han Xiao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The development of nanomedicines has recently achieved several breakthroughs in the field of cancer treatment; however, the biocompatibility and targeted burst release of these medications remain a limitation, which leads to serious side effects and significantly narrows the scope of their applications. The self-assembly of intermediate filament protein (IFP) peptides was triggered by a hydrophobic cation drug 7-amino actinomycin D (7-AAD) to synthesize pH-activatable nanoparticles (NPs) that could simultaneously locate tumors and produce antitumor effects. The designed IFP peptide included a target peptide (arginine–glycine–aspartate), a negatively charged region, and an α-helix sequence. It also possessed the ability to encapsulate 7-AAD molecules through the formation of hydrogen bonds and hydrophobic interactions by a one-step method. 7-AAD molecules with excellent near-infrared fluorescence properties could be target delivered into tumor cells by NPs and released immediately in the acidic environments of tumors and endosome/lysosomes, ultimately inducing cytotoxicity by arresting the tumor cell cycle with inserted DNA. It is noteworthy that the IFP/7-AAD NPs tail vein injection approach demonstrated not only high tumor-targeted imaging potential, but also strong antitumor therapeutic effects in vivo. The proposed strategy may be used in the delivery of cationic antitumor drugs for precise imaging and cancer therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=7-amino%20actinomycin%20D" title="7-amino actinomycin D">7-amino actinomycin D</a>, <a href="https://publications.waset.org/abstracts/search?q=intermediate%20filament%20protein" title=" intermediate filament protein"> intermediate filament protein</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticle" title=" nanoparticle"> nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=tumor%20image" title=" tumor image"> tumor image</a> </p> <a href="https://publications.waset.org/abstracts/130877/a-ph-activatable-nanoparticle-self-assembly-triggered-by-7-amino-actinomycin-d-demonstrating-superior-tumor-fluorescence-imaging-and-anticancer-performance" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/130877.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1220</span> Optimization of Assay Parameters of L-Glutaminase from Bacillus cereus MTCC1305 Using Artificial Neural Network</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20Singh">P. Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20M.%20Banik"> R. M. Banik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Artificial neural network (ANN) was employed to optimize assay parameters viz., time, temperature, pH of reaction mixture, enzyme volume and substrate concentration of L-glutaminase from Bacillus cereus MTCC 1305. ANN model showed high value of coefficient of determination (0.9999), low value of root mean square error (0.6697) and low value of absolute average deviation. A multilayer perceptron neural network trained with an error back-propagation algorithm was incorporated for developing a predictive model and its topology was obtained as 5-3-1 after applying Levenberg Marquardt (LM) training algorithm. The predicted activity of L-glutaminase was obtained as 633.7349 U/l by considering optimum assay parameters, viz., pH of reaction mixture (7.5), reaction time (20 minutes), incubation temperature (35˚C), substrate concentration (40mM), and enzyme volume (0.5ml). The predicted data was verified by running experiment at simulated optimum assay condition and activity was obtained as 634.00 U/l. The application of ANN model for optimization of assay conditions improved the activity of L-glutaminase by 1.499 fold. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bacillus%20cereus" title="Bacillus cereus">Bacillus cereus</a>, <a href="https://publications.waset.org/abstracts/search?q=L-glutaminase" title=" L-glutaminase"> L-glutaminase</a>, <a href="https://publications.waset.org/abstracts/search?q=assay%20parameters" title=" assay parameters"> assay parameters</a>, <a href="https://publications.waset.org/abstracts/search?q=artificial%20neural%20network" title=" artificial neural network"> artificial neural network</a> </p> <a href="https://publications.waset.org/abstracts/13443/optimization-of-assay-parameters-of-l-glutaminase-from-bacillus-cereus-mtcc1305-using-artificial-neural-network" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13443.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">429</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1219</span> Poly(N-Vinylcaprolactam) Based Degradable Microgels for Controlled Drug Delivery</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=G.%20Agrawal">G. Agrawal</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Agrawal"> R. Agrawal</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Pich"> A. Pich</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The pH and temperature responsive biodegradable poly(N-vinylcaprolactam) (PVCL) based microgels functionalized with itaconic acid (IA) units are prepared via precipitation polymerization for drug delivery applications. Volume phase transition temperature (VPTT) of the obtained microgels is influenced by both IA content and pH of the surrounding medium. The developed microgels can be degraded under acidic conditions due to the presence of hydrazone based crosslinking points inside the microgel network. The microgel particles are able to effectively encapsulate doxorubicin (DOX) drug and exhibit low drug leakage under physiological conditions. At low pH, rapid DOX release is observed due to the changes in electrostatic interactions along with the degradation of particles. The results of the cytotoxicity assay further display that the DOX-loaded microgel exhibit effective antitumor activity against HeLa cells demonstrating their great potential as drug delivery carriers for cancer therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=degradable" title="degradable">degradable</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20delivery" title=" drug delivery"> drug delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrazone%20linkages" title=" hydrazone linkages"> hydrazone linkages</a>, <a href="https://publications.waset.org/abstracts/search?q=microgels" title=" microgels"> microgels</a>, <a href="https://publications.waset.org/abstracts/search?q=responsive" title=" responsive"> responsive</a> </p> <a href="https://publications.waset.org/abstracts/78957/polyn-vinylcaprolactam-based-degradable-microgels-for-controlled-drug-delivery" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78957.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">313</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1218</span> In Vitro Study of Antioxidant Capacity of Chrysanthemum Indicum Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Puchita%20Chokcharoenying">Puchita Chokcharoenying</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Polyphenols are the most abundant antioxidants found in plants, and they are highly effective at scavenging oxidative free radicals. Antioxidants are substances found in medicinal plants to help prevent heart disease, stroke, and some cancers. This study focused on evaluating the flavonoids content of Chrysanthemum Indicum and determine their antioxidant capacity by using DPPH and ABTS radical scavenging capacity assay. The total flavonoid content of C. indicumextract was determined and expressed as quercetin equivalents (QE)/g measured by an aluminiumchloride colorimetric method. The results showed that the IC50 of C. indicum extract were 83.57μg/mL ± 0.875 and52.57μg/mL ± 0.632for DPPH and ABTS, respectively. C. indicumextract exhibited antioxidant activities as a concentration dependent manner. In the DPPH assay, vitamin C was used as a positive control, whereas Trolox was used as a positive control in the ABTS assay. In summary, C. indicum extract is rich in flavonoids, which have potent antioxidant properties. Thus, C. indicum extract is a good source of antioxidants and can be developed for medicinal purposes. Nevertheless, more research on the antioxidant activity of C. indicum extract and in vivo antioxidant studies are still needed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ABTS%20assay" title="ABTS assay">ABTS assay</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=chrysanthemum%20indicum" title=" chrysanthemum indicum"> chrysanthemum indicum</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH%20assay" title=" DPPH assay"> DPPH assay</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20flavonoid%20content" title=" total flavonoid content"> total flavonoid content</a> </p> <a href="https://publications.waset.org/abstracts/140860/in-vitro-study-of-antioxidant-capacity-of-chrysanthemum-indicum-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/140860.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">258</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1217</span> Performance of Non-toxic, Corrosion Resistant, and Lubricious Metalworking Fluids under Machining</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Pratap%20Singh%20Lodhi">Ajay Pratap Singh Lodhi</a>, <a href="https://publications.waset.org/abstracts/search?q=Deepak%20Kumar"> Deepak Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Vegetable oil-based environmentally friendly metalworking fluids (MWFs) are formulated. The tribological performance, cytotoxicity, and corrosion resistance of the formulated fluids (FFs) are evaluated and benchmarked with commercial mineral oil-based MWFs (CF). Results show that FFs exhibited better machining characteristics (roughness, cutting forces, and surface morphology) during machining than CF. MTT assay and Live dead cell assay confirm the cytocompatibility nature of the FFs relative to the toxic CF. Electrochemical analysis shows that FFs and CF exhibited comparable corrosion current density. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=corrosion%20inhibitors" title="corrosion inhibitors">corrosion inhibitors</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=machining" title=" machining"> machining</a>, <a href="https://publications.waset.org/abstracts/search?q=MTT%20assay" title=" MTT assay"> MTT assay</a>, <a href="https://publications.waset.org/abstracts/search?q=Taguchi%20method" title=" Taguchi method"> Taguchi method</a>, <a href="https://publications.waset.org/abstracts/search?q=vegetable%20oil" title=" vegetable oil"> vegetable oil</a> </p> <a href="https://publications.waset.org/abstracts/144907/performance-of-non-toxic-corrosion-resistant-and-lubricious-metalworking-fluids-under-machining" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144907.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1216</span> Synthesis, Characterization and in vitro DNA Binding and Cleavage Studies of Cu(II)/Zn(II) Dipeptide Complexes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Jamsheera">A. Jamsheera</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Arjmand"> F. Arjmand</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20K.%20Mohapatra"> D. K. Mohapatra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Small molecules binding to specific sites along DNA molecule are considered as potential chemotherapeutic agents. Their role as mediators of key biological functions and their unique intrinsic properties make them particularly attractive therapeutic agents. Keeping in view, novel dipeptide complexes Cu(II)-Val-Pro (1), Zn(II)-Val-Pro (2), Cu(II)-Ala-Pro (3) and Zn(II)-Ala-Pro (4) were synthesized and thoroughly characterized using different spectroscopic techniques including elemental analyses, IR, NMR, ESI–MS and molar conductance measurements. The solution stability study carried out by UV–vis absorption titration over a broad range of pH proved the stability of the complexes in solution. In vitro DNA binding studies of complexes 1–4 carried out employing absorption, fluorescence, circular dichroism and viscometric studies revealed the binding of complexes to DNA via groove binding. UV–vis titrations of 1–4 with mononucleotides of interest viz., 5´-GMP and 5´-TMP were also carried out. The DNA cleavage activity of the complexes 1 and 2 were ascertained by gel electrophoresis assay which revealed that the complexes are good DNA cleavage agents and the cleavage mechanism involved a hydrolytic pathway. Furthermore, in vitro antitumor activity of complex 1 was screened against human cancer cell lines of different histological origin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dipeptide%20Cu%28II%29%20and%20Zn%28II%29%20complexes" title="dipeptide Cu(II) and Zn(II) complexes">dipeptide Cu(II) and Zn(II) complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20binding%20profile" title=" DNA binding profile"> DNA binding profile</a>, <a href="https://publications.waset.org/abstracts/search?q=pBR322%20DNA%20cleavage" title=" pBR322 DNA cleavage"> pBR322 DNA cleavage</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20anticancer%20activity" title=" in vitro anticancer activity"> in vitro anticancer activity</a> </p> <a href="https://publications.waset.org/abstracts/38418/synthesis-characterization-and-in-vitro-dna-binding-and-cleavage-studies-of-cuiiznii-dipeptide-complexes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38418.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">349</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1215</span> Effect of a Synthetic Platinum-Based Complex on Autophagy Induction in Leydig TM3 Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ezzati%20Givi%20M.">Ezzati Givi M.</a>, <a href="https://publications.waset.org/abstracts/search?q=Hoveizi%20E."> Hoveizi E.</a>, <a href="https://publications.waset.org/abstracts/search?q=Nezhad%20Marani%20N."> Nezhad Marani N.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Platinum-based anticancer therapeutics are the most widely used drugs in clinical chemotherapy but have major limitations and various side effects in clinical applications. Gonadotoxicity and sterility is one of the most common complications for cancer survivors, which seem to be drug-specific and dose-related. Therefore, many efforts have been dedicated to discovering a new structure of platinum-based anticancer agents with improved therapeutic index, fewer side effects. In this regard, new Pt(II)-phosphane complexes containing heterocyclic thionate ligands (PCTL) have been synthesized, which show more potent antitumor activities in comparison to cisplatin. Cisplatin, the best leading metal-based antitumor drug in the field, induces testicular toxicity on Leydig and Sertoli cells leading to serious side effects such as azoospermia and infertility. Therefore in the present study, we aimed to investigate the cytotoxicity effect of PCTL on mice TM4 Sertoli cells with particular emphasis on the role of autophagy in comparison to cisplatin. In this study, an MTT assay was performed to evaluate the IC50 of PCTL and to analyze the TM3 Leydig cell's viability. Cells morphology was evaluated via invert microscope and Changing in morphology for nuclei swelling or autophagic vacuoles formation were assessed by DAPI and MDC staining. Testosterone production in the culture medium was measured using an ELISA kit. Finally, the expression of Autophagy-related genes, Atg5, Beclin1 and p62, were analyzed by qPCR. Based on the obtained results by MTT, the IC50 value of PCTL was 50 μM in TM3 cells and cytotoxic effects was in a dose- and time-dependent manner. Cells morphological changes investigated by inverted microscopy, DAPI, and MDC staining which showed the cytotoxic concentrations of PCTL was significantly higher than cisplatin in the treated TM3 Leydig cells. The results of PCR showed a lack of expression of the p62, Atg5 and Beclin1 gene in TM3 cells treated with PCTL in comparison to cisplatin and control groups. It should be noted that the effects of 25 μM PCTL concentration on TM3 cells have been associated with increased testosterone production and secretion, which requires further study to explain the possible causes and involved molecular mechanisms. The results of the study showed that the PCTL had less-lethal effects on TM3 cells in comparison to cisplatin and probably did not induce autophagy in TM3 cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=platinum-based%20anticancer%20agents" title="platinum-based anticancer agents">platinum-based anticancer agents</a>, <a href="https://publications.waset.org/abstracts/search?q=cisplatin" title=" cisplatin"> cisplatin</a>, <a href="https://publications.waset.org/abstracts/search?q=Leydig%20TM3%20cells" title=" Leydig TM3 cells"> Leydig TM3 cells</a>, <a href="https://publications.waset.org/abstracts/search?q=autophagy" title=" autophagy"> autophagy</a> </p> <a href="https://publications.waset.org/abstracts/149103/effect-of-a-synthetic-platinum-based-complex-on-autophagy-induction-in-leydig-tm3-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149103.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">128</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1214</span> Utility of the Loop-Mediated Isothermal Amplification Assay for the Diagnosis of Visceral Leishmaniasis from Blood Samples in Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dawit%20Gebreegzabher%20Hagos">Dawit Gebreegzabher Hagos</a>, <a href="https://publications.waset.org/abstracts/search?q=Yazezew%20Kebede%20Kiro"> Yazezew Kebede Kiro</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmud%20Abdulkader"> Mahmud Abdulkader</a>, <a href="https://publications.waset.org/abstracts/search?q=Henk%20H.%20D.%20F.%20Schallig"> Henk H. D. F. Schallig</a>, <a href="https://publications.waset.org/abstracts/search?q=Dawit%20Wolday"> Dawit Wolday</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapid and accurate visceral leishmaniasis (VL) diagnosis is needed to initiate prompt treatment to reduce morbidity and mortality. Here, we evaluated the performance of loop-mediated isothermal amplification (LAMP) assay for the diagnosis of VL from blood in an endemic area in Ethiopia. LAMP was positive in 117/122 confirmed VL cases and negative in 149/152 controls, resulting in a sensitivity of 95.9% (95% CI: 90.69–98.66) and a specificity of 98.0% (95% CI: 94.34–99.59), respectively. The sensitivity of the LAMP assay was 95.0% (95% CI: 88.61–98.34) in HIV-negatives and 100% (95% CI: 85.18–100.0) in HIV-positives. Compared with microscopy, LAMP detected 82/87 (94.3%, 95% CI: 87.10–98.11) of the microscopy1 cases and was negative in 11/27 (40.7%, 95% CI: 22.39–61.20) of the microscopy2 cases. Compared with the rK39 serology, LAMP detected 113/120 (94.2%, 95% CI: 88.35–97.62) of the rK391 cases and was negative in 149/154 (96.8%, 95% CI: 92.59–98.94) of the rK392 cases. However, when compared with microscopy only, rK39 detected 83/87 (95.4%, 95% CI: 88.64–98.73) of the microscopy1 cases and negative in only 12/27 (44.4%, 95% CI: 25.48–64.67) of the microscopy– cases. There was an excellent agreement between rK39 and LAMP (Kappa 5 0.91, 95% CI: 0.86–0.96). Furthermore, an algorithm using rK39 followed by LAMP would yield a sensitivity of 99.2% (95%CI: 95.52–99.89) and a specificity of 98.0% (95% CI: 94.34–99.59). The findings demonstrate that the LAMP assay is an accurate and rapid molecular assay for VL diagnosis, including in HIV-1 co-infected patients, in an endemic setting. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=visceral%20leishmaniasis" title="visceral leishmaniasis">visceral leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=HIV" title=" HIV"> HIV</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=LAMP" title=" LAMP"> LAMP</a>, <a href="https://publications.waset.org/abstracts/search?q=Ethiopia" title=" Ethiopia"> Ethiopia</a> </p> <a href="https://publications.waset.org/abstracts/162163/utility-of-the-loop-mediated-isothermal-amplification-assay-for-the-diagnosis-of-visceral-leishmaniasis-from-blood-samples-in-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162163.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">98</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1213</span> Evaluation Of In Vitro Antioxidant Potential of Camellia Sinensis Leaves Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jirathan%20Pongchababnapa">Jirathan Pongchababnapa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Polyphenols are the most common antioxidant found in plants and are efficient in capturing oxidative free radicals. Antioxidants are substances found in medicinal plants which may have a protective role to play in certain conditions such as heart disease, stroke and some cancers. By relying on these benefits, we have traced out the presence of antioxidant in Camellia sinensis leaves extract. This study aims to evaluate flavonoids content in C. sinensisextract and investigate antioxidant activities by using DPPH and ABTS radical scavenging capacity assay. The total flavonoid content of C. Sinensis extract was determined and expressed as quercetin equivalents (QE)/g measured by the aluminum chloride colorimetric method. The results showed that the IC₅₀ of C. Sinensis leaves extract were 40.90 μg/mL ± 0.755 and32.96 μg/mL ± 0.679 for DPPH and ABTS, respectively. C. Sinensis extract at increasing concentration showed antioxidant activities as a concentration dependent manner. In the DPPH assay, vitamin C was used as a positive control, whereas Trolox was used as a positive control in the ABTS assay. In conclusion, C. Sinensis extract consisted of a high amount of flavonoids content which possesses potent antioxidant activity. However, further investigation on the identification of pure compound of this plant and molecular antioxidant assays are still required. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ABTS%20assay" title="ABTS assay">ABTS assay</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=camellia%20sinensis" title=" camellia sinensis"> camellia sinensis</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH%20assay" title=" DPPH assay"> DPPH assay</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20flavonoid%20content" title=" total flavonoid content"> total flavonoid content</a> </p> <a href="https://publications.waset.org/abstracts/140929/evaluation-of-in-vitro-antioxidant-potential-of-camellia-sinensis-leaves-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/140929.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">210</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1212</span> Invitro Study of Anti-Leishmanial Property of Nigella Sativa Methanalic Black Seed Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tawqeer%20Ali%20Syed">Tawqeer Ali Syed</a>, <a href="https://publications.waset.org/abstracts/search?q=Prakash%20Chandra"> Prakash Chandra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study aims to evaluate the antileishmanial activity of Nigella sativa black seed extract. This well-known plant extract was taken from the botanical garden of Kashmir. Materials and Methods: The methanolic extracts of these plants were screened for their antileishmanial activity against Leishmania major using 3‑(4.5‑dimethylthiazol‑2yl)‑2.5‑diphenyltetrazolium bromide assay or MTT assay. Results: The methanolic extract of Nigella sativa showed potential antileishmanial activity at an inhibition% value of 80.29% ± 0.65%. IC 50 was calculated after 48 hours to be 964.3 µg/ml. Conclusion: Considering these results, these medicinal plants from Kashmir could serve as potential drug sources for antileishmanial compounds. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=MTT%20assay" title="MTT assay">MTT assay</a>, <a href="https://publications.waset.org/abstracts/search?q=antileishmanial" title=" antileishmanial"> antileishmanial</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20viability" title=" cell viability"> cell viability</a>, <a href="https://publications.waset.org/abstracts/search?q=Nigella%20sativa" title=" Nigella sativa"> Nigella sativa</a> </p> <a href="https://publications.waset.org/abstracts/138432/invitro-study-of-anti-leishmanial-property-of-nigella-sativa-methanalic-black-seed-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138432.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">213</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1211</span> Optimizing Fermented Paper Production Using Spyrogira sp. Interpolating with Banana Pulp</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hadiatullah">Hadiatullah</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20S.%20D.%20Desak%20Ketut"> T. S. D. Desak Ketut</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Ayu"> A. A. Ayu</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20N.%20Isna"> A. N. Isna</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20P.%20Ririn"> D. P. Ririn </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Spirogyra sp. is genus of microalgae which has a high carbohydrate content that used as a best medium for bacterial fermentation to produce cellulose. This study objective to determine the effect of pulp banana in the fermented paper production process using Spirogyra sp. and characterizing of the paper product. The method includes the production of bacterial cellulose, assay of the effect fermented paper interpolating with banana pulp using Spirogyra sp., and the assay of paper characteristics include gram-mage paper, water assay absorption, thickness, power assay of tensile resistance, assay of tear resistance, density, and organoleptic assay. Experiments were carried out with completely randomized design with a variation of the concentration of sewage treatment in the fermented paper production interpolating banana pulp using Spirogyra sp. Each parameter data to be analyzed by Anova variance that continued by real difference test with an error rate of 5% using the SPSS. Nata production results indicate that different carbon sources (glucose and sugar) did not show any significant differences from cellulose parameters assay. Significantly different results only indicated for the control treatment. Although not significantly different from the addition of a carbon source, sugar showed higher potency to produce high cellulose. Based on characteristic assay of the fermented paper showed that the paper gram-mage indicated that the control treatment without interpolation of a carbon source and a banana pulp have better result than banana pulp interpolation. Results of control gram-mage is 260 gsm that show optimized by cardboard. While on paper gram-mage produced with the banana pulp interpolation is about 120-200 gsm that show optimized by magazine paper and art paper. Based on the density, weight, water absorption assays, and organoleptic assay of paper showing the highest results in the treatment of pulp banana interpolation with sugar source as carbon is 14.28 g/m2, 0.02 g and 0.041 g/cm2.minutes. The conclusion found that paper with nata material interpolating with sugar and banana pulp has the potential formulation to produce super-quality paper. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cellulose" title="cellulose">cellulose</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=grammage" title=" grammage"> grammage</a>, <a href="https://publications.waset.org/abstracts/search?q=paper" title=" paper"> paper</a>, <a href="https://publications.waset.org/abstracts/search?q=Spirogyra%20sp." title=" Spirogyra sp."> Spirogyra sp.</a> </p> <a href="https://publications.waset.org/abstracts/32790/optimizing-fermented-paper-production-using-spyrogira-sp-interpolating-with-banana-pulp" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32790.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">333</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1210</span> Sider Bee Honey: Antitumor Effect in Some Experimental Tumor Cell Lines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aliaa%20M.%20Issa">Aliaa M. Issa</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20N.%20ElRouby"> Mahmoud N. ElRouby</a>, <a href="https://publications.waset.org/abstracts/search?q=Sahar%20A.%20S.%20Ahmad"> Sahar A. S. Ahmad</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20M.%20El-Merzabani"> Mahmoud M. El-Merzabani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Sider honey is a type of honey produced by bees feeding on the nectar of Sider tree, Ziziphus spina-christi (L) Desf . Honey is an effective agent for preventing, inhibiting and treating the growth of human and animal cancer cell lines in vitro and in vivo. The aim of the present study was to evaluate the impact of different dilutions from crude Sider honey and different duration times of exposure on the growth of six tumor cell lines (human cervical cancer cell line, HeLa; human hepatocellular carcinoma cell line, HepG-2; human larynx carcinoma cell line, Hep-2; brain tumor cell line, U251) as well as one animal cancerous cell line (Ehrlich ascites carcinoma cells line, EAC) and one normal cell line, Homo sapiens, human, (WISH) CCL-25. Different concentrations and treatment durations with Sider honey were tested on the growth of several cancer cell lines types. Histopathological changes in the tumor masses, animal survival, apoptosis and necrosis of the used cancer cell lines (using flow cytometry) were evaluated. Sider honey was administers either to the tumor mass itself by intratumoral injection or via drinking water. One-way ANOVA test was used for the analysis of (the means + standard error) of the optical density obtained from the Elisa reader and flow cytometry. The study revealed that different concentrations of Sider honey affected the growth patterns of all the studied cancer cell lines as well as their histopathological changes, and it depended on the cell line nature and the concentration of honey used. It is obvious that the relative animal survival percentage (bearing Ehrlich ascites carcinoma, EAC cells) was proportionally increased with the increase in the used honey concentrations. The study of apoptosis and necrosis using the flow cytometry technique emphasized the viability results. In conclusion, Sider honey was effective as antitumor agent, in the used concentrations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antitumor" title="antitumor">antitumor</a>, <a href="https://publications.waset.org/abstracts/search?q=honey" title=" honey"> honey</a>, <a href="https://publications.waset.org/abstracts/search?q=sider" title=" sider"> sider</a>, <a href="https://publications.waset.org/abstracts/search?q=tumor%20cell%20lines" title=" tumor cell lines"> tumor cell lines</a> </p> <a href="https://publications.waset.org/abstracts/41053/sider-bee-honey-antitumor-effect-in-some-experimental-tumor-cell-lines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41053.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">537</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1209</span> In vitro Antioxidant Activity of Caesalpinia sappan Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Monthon%20Tangjitmungman">Monthon Tangjitmungman</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Numerous diseases have been linked to oxidative stress, in which a disproportion of free radicals in the body leads to tissue or cell damage. Polyphenols are the most abundant antioxidants found in plants, and they are highly effective at scavenging oxidative free radicals. Due to the presence of phenolic compounds in Caesalpinia sappan has been discovered to have antioxidant activity. It has several health benefits, the most important of which is preventing cardiovascular and cancer diseases. This study aimed to determine the phenolic content and antioxidant activity of C. sappan extract using a variety of antioxidant assays. The extract of C. sappan was made using a mixture of solvents (ethyl alcohol: water in ratio 8:2). The total phenolic content of C. sappan extract was determined and expressed as gallic acid equivalents using the Folin-Cioucalteu method (GAE). The antioxidant activity of C. sappan extract was assessed using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the ABTS radical scavenging capacity assay. An association was found between antioxidant activity and total phenol content. The antioxidant activity of C. sappan extract was also determined by DPPH and ABTS assays. The IC50 values for C. sappan extract from DPPH and ABTS assays were 54.48 μg/mL ± 0.545 and 25.46 μg/mL ± 0.790, respectively, in the DPPH assay. In the DPPH assay, vitamin C was used as a positive control, whereas Trolox was used as a positive control in the ABTS assay. In conclusion, C. sappan extract contains a high level of total phenolics and exhibits significant antioxidant activity. Nevertheless, more research should be done on the antioxidant activity, such as SOD and ROS scavenging assays and in vivo experiments, to determine whether the compound has antioxidant activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ABTS%20assay" title="ABTS assay">ABTS assay</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Caesalpinia%20sappan" title=" Caesalpinia sappan"> Caesalpinia sappan</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH%20assays" title=" DPPH assays"> DPPH assays</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20phenolic%20content" title=" total phenolic content"> total phenolic content</a> </p> <a href="https://publications.waset.org/abstracts/140865/in-vitro-antioxidant-activity-of-caesalpinia-sappan-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/140865.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">384</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1208</span> Novel Nanomagnetic Beads Based- Latex Agglutination Assay for Rapid Diagnosis of Human Schistosomiasis Haematobium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20Aly">Ibrahim Aly</a>, <a href="https://publications.waset.org/abstracts/search?q=Rabab%20Zalat"> Rabab Zalat</a>, <a href="https://publications.waset.org/abstracts/search?q=Bahaa%20EL%20Deen%20W.%20El%20Aswad"> Bahaa EL Deen W. El Aswad</a>, <a href="https://publications.waset.org/abstracts/search?q=Ismail%20M.%20Moharm"> Ismail M. Moharm</a>, <a href="https://publications.waset.org/abstracts/search?q=Basam%20M.%20Masoud"> Basam M. Masoud</a>, <a href="https://publications.waset.org/abstracts/search?q=Tarek%20Diab"> Tarek Diab</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of the present study was to evaluate the novel nanomagnetic beads based–latex agglutination assay (NMB-LAT) as a simple test for diagnosis of S. haematobium as well as standardize the novel nanomagnetic beads based –ELISA (NMB-ELISA). According to urine examination this study included 85 S. haematobium infected patients, 30 other parasites infected patients and 25 negative control samples. The sensitivity of novel NMB-LAT was 82.4% versus 96.5% and 88.2% for NMB-ELISA and currently used sandwich ELISA respectively. The specificity of NMB-LAT was 83.6% versus 96.3% and 87.3% for NMB-ELISA and currently used sandwich ELISA respectively. In conclusion, the novel NMB-ELISA is a valuable applicable diagnostic technique for diagnosis of human schistosomiasis haematobium. The novel NMB-ELISA assay is a suitable applicable diagnostic method in field survey especially when followed by ELISA as a confirmatory test in query false negative results. Trials are required to increase the sensitivity and specificity of NMB-ELISA assay. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title="diagnosis">diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=iatex%20agglutination" title=" iatex agglutination"> iatex agglutination</a>, <a href="https://publications.waset.org/abstracts/search?q=nanomagnetic%20beads" title=" nanomagnetic beads"> nanomagnetic beads</a>, <a href="https://publications.waset.org/abstracts/search?q=sandwich%20ELISA" title=" sandwich ELISA"> sandwich ELISA</a> </p> <a href="https://publications.waset.org/abstracts/2898/novel-nanomagnetic-beads-based-latex-agglutination-assay-for-rapid-diagnosis-of-human-schistosomiasis-haematobium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2898.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">382</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1207</span> Synthesis, Characterization and Bioactivity of Methotrexate Conjugated Fluorescent Carbon Nanoparticles in vitro Model System Using Human Lung Carcinoma Cell Lines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdul%20Matin">Abdul Matin</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Ajmal"> Muhammad Ajmal</a>, <a href="https://publications.waset.org/abstracts/search?q=Uzma%20Yunus"> Uzma Yunus</a>, <a href="https://publications.waset.org/abstracts/search?q=Noaman-ul%20Haq"> Noaman-ul Haq</a>, <a href="https://publications.waset.org/abstracts/search?q=Hafiz%20M.%20Shohaib"> Hafiz M. Shohaib</a>, <a href="https://publications.waset.org/abstracts/search?q=Ambreen%20G.%20Muazzam"> Ambreen G. Muazzam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Carbon nanoparticles (CNPs) have unique properties that are useful for the diagnosis and treatment of cancer due to their precise properties like small size (ideal for delivery within the body) stability in solvent and tunable surface chemistry for targeted delivery. Here, highly fluorescent, monodispersed and water-soluble CNPs were synthesized directly from a suitable carbohydrate source (glucose and sucrose) by one-step acid assisted ultrasonic treatment at 35 KHz for 4 hours. This method is green, simple, rapid and economical and can be used for large scale production and applications. The average particle sizes of CNPs are less than 10nm and they emit bright and colorful green-blue fluorescence under the irradiation of UV-light at 365nm. The CNPs were characterized by scanning electron microscopy, fluorescent spectrophotometry, Fourier transform infrared spectrophotometry, ultraviolet-visible spectrophotometry and TGA analysis. Fluorescent CNPs were used as fluorescent probe and nano-carriers for anticancer drug. Functionalized CNPs (with ethylene diamine) were attached with anticancer drug-Methotrexate. In vitro bioactivity and biocompatibility of CNPs-drug conjugates was evaluated by LDH assay and Sulforhodamine B assay using human lung carcinoma cell lines (H157). Our results reveled that CNPs showed biocompatibility and CNPs-anticancer drug conjugates have shown potent cytotoxic effects and high antitumor activities in lung cancer cell lines. CNPs are proved to be excellent substitute for conventional drug delivery cargo systems and anticancer therapeutics in vitro. Our future studies will be more focused on using the same nanoparticles in vivo model system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=carbon%20nanoparticles" title="carbon nanoparticles">carbon nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=carbon%20nanoparticles-methotrexate%20conjugates" title=" carbon nanoparticles-methotrexate conjugates"> carbon nanoparticles-methotrexate conjugates</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20lung%20carcinoma%20cell%20lines" title=" human lung carcinoma cell lines"> human lung carcinoma cell lines</a>, <a href="https://publications.waset.org/abstracts/search?q=lactate%20dehydrogenase" title=" lactate dehydrogenase"> lactate dehydrogenase</a>, <a href="https://publications.waset.org/abstracts/search?q=methotrexate" title=" methotrexate"> methotrexate</a> </p> <a href="https://publications.waset.org/abstracts/42526/synthesis-characterization-and-bioactivity-of-methotrexate-conjugated-fluorescent-carbon-nanoparticles-in-vitro-model-system-using-human-lung-carcinoma-cell-lines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42526.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">305</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1206</span> Prevalence and Evaluation of Antimicrobial Activity of Dodonaea viscosa Extract and Antibacterial Agents against Salmonella spp. Isolated from Poultry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shayma%20Munqith%20Al-Baker">Shayma Munqith Al-Baker</a>, <a href="https://publications.waset.org/abstracts/search?q=Fadhl%20Ahmed%20Saeed%20Al-Gasha%E2%80%99a"> Fadhl Ahmed Saeed Al-Gasha’a</a>, <a href="https://publications.waset.org/abstracts/search?q=Samira%20Hamid%20Hanash"> Samira Hamid Hanash</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Ali%20Al-Hazmi"> Ahmed Ali Al-Hazmi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A total of 200 samples (180 fecal materials and 20 organ samples) were collected from (5 different poultry farms, 10 local poultry shops, 5 houses poultry, 5 Eggs stores shops and 5 hand slaughters centers) in Ibb city, Yemen, 2014. According to morphological, cultural, as well as biochemical characterization and serological tests, 59 29.5% isolates were identified as Salmonella spp. and all Salmonella isolates were categorized by serotype, which comprised of, 37 62.71% Salmonella Typhimurium serovar, 21 35.59%. Salmonella Enteritidis serovar and 11.69% Salmonella Heidelberg serovar. Antibiotic sensitivity test was done for bacterial isolates and the results showed there were clear differences in antibiotic resistant. Antimicrobial susceptibility of the isolates varies as follows: Ofloxacin 79.66%, Ciprofloxacin 67.80%, Colistin 59.32% and Gentamycin 52.54%. All of isolates were resistant to Erythromycin, Penicillin and Lincomycin. Antibacterial activity was done for both aqueous and ethanol extracts of Dodonaea viscosa plant by using well and disc diffusion assay. The results indicated that well diffusion assay had best results than disc diffusion assay, the highest inhibition zone was 22 mm for well diffusion and 15 mm for disc diffusion assay, the results observed that ethanol extract had best antibacterial effect than aqueous extract which the percentage of bacterial isolates affected with ethanol extract was 71.19% comparing with aqueous extract 28.81% by using disc diffusion assay, while the percentage of bacterial isolates affected with ethanol extract was 88.13% comparing with aqueous extract 52.54% by using will diffusion assay. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Salmonella%20spp" title="Salmonella spp">Salmonella spp</a>, <a href="https://publications.waset.org/abstracts/search?q=Dodonaea%20viscosa" title=" Dodonaea viscosa"> Dodonaea viscosa</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20and%20salmonellosis" title=" antimicrobial and salmonellosis"> antimicrobial and salmonellosis</a> </p> <a href="https://publications.waset.org/abstracts/26871/prevalence-and-evaluation-of-antimicrobial-activity-of-dodonaea-viscosa-extract-and-antibacterial-agents-against-salmonella-spp-isolated-from-poultry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26871.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">474</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1205</span> Mutations in rpoB, katG and inhA Genes: The Association with Resistance to Rifampicin and Isoniazid in Egyptian Mycobacterium tuberculosis Clinical Isolates</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayman%20K.%20El%20Essawy">Ayman K. El Essawy</a>, <a href="https://publications.waset.org/abstracts/search?q=Amal%20M.%20Hosny"> Amal M. Hosny</a>, <a href="https://publications.waset.org/abstracts/search?q=Hala%20M.%20Abu%20Shady"> Hala M. Abu Shady</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The rapid detection of TB and drug resistance, both optimizes treatment and improves outcomes. In the current study, respiratory specimens were collected from 155 patients. Conventional susceptibility testing and MIC determination were performed for rifampicin (RIF) and isoniazid (INH). Genotype MTBDRplus assay, which is a molecular genetic assay based on the DNA-STRIP technology and specific gene sequencing with primers for rpoB, KatG, and mab-inhA genes were used to detect mutations associated with resistance to rifampicin and isoniazid. In comparison to other categories, most of rifampicin resistant (61.5%) and isoniazid resistant isolates (47.1%) were from patients relapsed in treatment. The genotypic profile (using Genotype MTBDRplus assay) of multi-drug resistant (MDR) isolates showed missing of katG wild type 1 (WT1) band and appearance of mutation band katG MUT2. For isoniazid mono-resistant isolates, 80% showed katG MUT1, 20% showed katG MUT1, and inhA MUT1, 20% showed only inhA MUT1. Accordingly, 100% of isoniazid resistant strains were detected by this assay. Out of 17 resistant strains, 16 had mutation bands for katG distinguished high resistance to isoniazid. The assay could clearly detect rifampicin resistance among 66.7% of MDR isolates that showed mutation band rpoB MUT3 while 33.3% of them were considered as unknown. One mono-resistant rifampicin isolate did not show rifampicin mutation bands by Genotype MTBDRplus assay, but it showed an unexpected mutation in Codon 531 of rpoB by DNA sequence analysis. Rifampicin resistance in this strain could be associated with a mutation in codon 531 of rpoB (based on molecular sequencing), and Genotype MTBDRplus assay could not detect the associated mutation. If the results of Genotype MTBDRplus assay and sequencing were combined, this strain shows hetero-resistance pattern. Gene sequencing of eight selected isolates, previously tested by Genotype MTBDRplus assay, could detect resistance mutations mainly in codon 315 (katG gene), position -15 in inhA promotes gene for isoniazid resistance and codon 531 (rpoB gene) for rifampicin resistance. Genotyping techniques allow distinguishing between recurrent cases of reinfection or reactivation and supports epidemiological studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20tuberculosis" title="M. tuberculosis">M. tuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=rpoB" title=" rpoB"> rpoB</a>, <a href="https://publications.waset.org/abstracts/search?q=KatG" title=" KatG"> KatG</a>, <a href="https://publications.waset.org/abstracts/search?q=inhA" title=" inhA"> inhA</a>, <a href="https://publications.waset.org/abstracts/search?q=genotype%20MTBDRplus" title=" genotype MTBDRplus"> genotype MTBDRplus</a> </p> <a href="https://publications.waset.org/abstracts/115843/mutations-in-rpob-katg-and-inha-genes-the-association-with-resistance-to-rifampicin-and-isoniazid-in-egyptian-mycobacterium-tuberculosis-clinical-isolates" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/115843.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">167</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1204</span> Evaluation of Antioxidant and Anticancer Activity of Tinospora cordifolia against Ehrlich Ascites Carcinoma: In Vitro, in vivo and in silico Approach</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anik%20Barua">Anik Barua</a>, <a href="https://publications.waset.org/abstracts/search?q=Rabiul%20Hossain"> Rabiul Hossain</a>, <a href="https://publications.waset.org/abstracts/search?q=Labonno%20Barua"> Labonno Barua</a>, <a href="https://publications.waset.org/abstracts/search?q=Rashadul%20Hossain"> Rashadul Hossain</a>, <a href="https://publications.waset.org/abstracts/search?q=Nurul%20Absar"> Nurul Absar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Globally, the burden of cancer is increasing consistently. Modern cancer therapies include lots of toxicity in the non-targeted organs reducing the life expectancy of the patients. Hence, scientists are trying to seek noble compounds from natural sources to treat cancer. Objectives: The objectives of the present study are to evaluate the phytochemicals, in vitro antioxidants, and in vivo and in silico anticancer study of various solvent fractions of Tinospora cordifolia (Willd.). Methodology: In this experiment, standard quantitative and qualitative assay methods were used to analyze the phytochemicals. The antioxidant activity was measured using the DPPH and ABTS scavenging methods. The in vivo antitumor activity is evaluated against Ehrlich ascites carcinoma (EAC) cell bearing in Swiss albino mice. In-silico ADME/T and molecular docking study were performed to assess the potential of stated phytochemicals against Transcription Factor STAT3b/DNA Complex of adenocarcinoma. Findings: Phytochemical screening confirmed the presence of flavonoids, alkaloids, glycosides, tannins, and carbohydrates. A significant amount of phenolic (20.19±0.3 mg/g GAE) and flavonoids (9.46±0.18 mg/g GAE) were found in methanolic extract in quantitative screening. Tinospora cordifolia methanolic extract showed promising DPPH and ABTS scavenging activity with the IC50 value of 1222.99 µg/mL and 1534.34 µg/mL, respectively, which was concentration dependent. In vivo anticancer activity in EAC cell-bearing mice showed significant (P < 0.05) percent inhibition of cell growth (60.12±1.22) was found at the highest dose compared with standard drug 5-Fluorouracil (81.18±1.28). Forty-two phytochemicals exhibit notable pharmacokinetics properties and passed drug-likeness screening tests in silico. In molecular docking study, (25S)-3Beta-acetoxy-5-alpha-22-beta-spirost-9(11)-en-12-beta-ol showed docking score (-8.5 kJ/mol) with significant non-bonding interactions with target enzyme. Conclusions: The results were found to be significant and confirmed that the methanolic extract of Tinospora cordifolia has remarkable antitumor activity with antioxidant potential. The Tinospora cordifolia methanolic extract may be considered a potent anticancer agent for advanced research. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anticancer" title="anticancer">anticancer</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=Tinospora%20cordifolia" title=" Tinospora cordifolia"> Tinospora cordifolia</a>, <a href="https://publications.waset.org/abstracts/search?q=EAC%20cell" title=" EAC cell"> EAC cell</a> </p> <a href="https://publications.waset.org/abstracts/157005/evaluation-of-antioxidant-and-anticancer-activity-of-tinospora-cordifolia-against-ehrlich-ascites-carcinoma-in-vitro-in-vivo-and-in-silico-approach" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157005.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">129</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=antitumor%20assay&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=antitumor%20assay&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=antitumor%20assay&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=antitumor%20assay&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=antitumor%20assay&page=6">6</a></li> <li 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