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border-color; -o-transition-property: border-color; transition-property: border-color; } /* Style new checked item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ padding-right: 10px; } .hbspt-form .hs-lastname, .kits_form_col .hs_confirm_email{ padding-left: 10px; } .kits_form_col {padding-bottom: 108px;} .hs-richtext h4 {margin-bottom: 30px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 20px;} } @media screen and (max-width: 640px){ .hbspt-form h1{font-size: 32px !important;} .hbspt-form .gform_heading p{font-size: 16px !important;} .hbspt-form .form-columns-2 .field{ -webkit-box-flex: 0; -ms-flex: 0 0 100%; flex: 0 0 100%; margin-bottom: .5rem; padding-left: 0; } } @media screen and (max-width: 540px){ .event-hero-section > div{ justify-items: flex-start !important; } } @media screen and (max-width: 414px){ body .hubspot_signup_form .hs_email {width: 66% !important;} body .hubspot_signup_form ul.no-list{top: 65px !important;} } @media screen and (max-width: 320px){ body .hubspot_signup_form .hs_email {width: 61% !important;} }</style> <style type="text/css">.about-presenter-section{display:none;} .space-hero-section{margin-bottom:0px !important;} .partner_bioAgilytix {padding-right: 2.5rem !important;} @media screen and (max-width: 540px){ .event-hero-section > div{ justify-items: flex-start !important; } }</style> <style type="text/css">/* Add your CSS code here. 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class="masthead__content-body"><h1>qPCR and ddPCR Assay Services for Accurate Gene Expression Analysis</h1><div class="masthead__buttons"> <a class="btn masthead__button btn--size-md" href="https://www.bioagilytix.com/speak-to-scientist/">Speak to a Scientist</a></div></div></div><div class="breadcrumbs"><span><span><a href="https://www.bioagilytix.com/"><i class="home-icon"></i><span class="screen-reader-text">Home</span></a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/">Techniques & Platforms</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/instrumentation/">Instrumentation</a></span> <span class="breadcrumb-separator"></span> <span class="breadcrumb_last" aria-current="page">PCR</span></span></div></div></section><div class="wp-block-group alignwide is-layout-constrained wp-container-core-group-is-layout-1 wp-block-group-is-layout-constrained"><p class="has-text-align-center has-large-font-size">BioAgilytix offers Real-Time PCR (RT-PCR), Quantitative PCR (qPCR), and Droplet Digital™ PCR (ddPCR™) platforms as part of our extensive technology suite supporting molecular assays. Our molecular laboratories are equipped with the latest generation of PCR equipment including Applied Biosystems QuantStudio 7 Flex or Pro with both 96- and 384-well fast-cycle options and TaqMan Array Cards for qPCR as well as QX200 and QX ONE ddPCR Systems from Bio-Rad.</p></div><div id="block_b90aee5aa4f9a469406cbfd256e8e001" class="wp-block em-media-content alignfull"><div class="em-media-content__inner em-media-content__inner--media-right has-gray-xxl-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--right"> <img fetchpriority="high" decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/rna-strand-1380x776.jpg" alt="rna strand" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/rna-strand-1380x776.jpg 1380w, https://www.bioagilytix.com/wp-content/uploads/2023/08/rna-strand-1120x630.jpg 1120w, https://www.bioagilytix.com/wp-content/uploads/2023/08/rna-strand-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/rna-strand-640x360.jpg 640w" width="1380" height="776"></div><div class="em-media-content__content em-media-content__content--left em-media-content__content--add-padding"><h2 class="h2"><strong>BioAgilytix’s PCR expertise</strong></h2><p class="">Our team of scientific experts collaborate closely with our clients to develop unique molecular solutions for various therapeutic programs.  We are well versed in the development, optimization, and validation of complex molecular assays using qPCR and ddPCR to deliver premier molecular services to support sponsors’ testing needs. </p><p class="">Our laboratory spaces are strategically designed to better accommodate PCR workflows with separate facilities for automated DNA and RNA extraction.  Our scientists have experience designing assays for a variety of sample types and are experts in biodistribution and persistence assays as well as viral shedding assays in support of ATMPs.  </p></div></div></div><div class="wp-block-emagine-grid alignwide wp-block-emagine-grid--halves em-mb--md"><div class="wp-block-emagine-grid__inner em-grid-2_sm-1-left-middle"><div class="wp-block-emagine-column em-col-middle"><div class="wp-block-image size-full"><div><figure class=""><noscript><img decoding="async" width="650" height="600" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7.png" alt="" class="wp-image-4288" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7.png 650w, https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7-300x277.png 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7-100x92.png 100w" sizes="(max-width: 650px) 100vw, 650px" /></noscript><img decoding="async" width="650" height="600" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%20650%20600%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7.png" alt="" class="lazyload wp-image-4288" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7.png 650w, https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7-300x277.png 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/viia-7-100x92.png 100w" data-sizes="(max-width: 650px) 100vw, 650px" /></figure></div></div></div><div class="wp-block-emagine-column em-col-middle"><h2 class="h2"><strong>PCR applications: For detecting single or multiple nucleic targets</strong></h2><p class="">BioAgilytix’s scientists utilize qPCR and ddPCR assays in our work with biomarkers, pharmacokinetics/pharmacodynamics (PK/PD), and for the bioanalysis of Advanced Therapy Medicinal Products (ATMPs) programs. PCR is an essential method for detecting nucleic acids and has a wide range of clinical and research applications. Our state-of-the-art qPCR and ddPCR systems allow us to customize molecular solutions for our clients using robust methodologies in a streamlined, efficient manner.</p></div><div class="wp-block-emagine-column em-col"><h3 class="h3"><strong>How the qPCR platforms work</strong></h3><p class="">qPCR tracks the amplification of DNA or RNA (cDNA) in real time through continual measurements of fluorescent molecules (chromophores) activated during the PCR process. For qPCR assays, we leverage the Applied Biosystems QuantStudio technology, which provides enhanced, high-resolution fluorescence detection for precise quantification with multiplexing capabilities. Our molecular suite is equipped with the latest generation of qPCR equipment, including Applied Biosystems QuantStudio 7 Flex or Pro with both 96- and 384-well fast-cycle options as well as TaqMan Array Cards. Our modern qPCR instrumentation allows for the simultaneous measurement of multiple DNA amplification reactions in a single reaction vessel, making it possible to process large batches of samples and multiplex endpoints to increase throughput. This enables reaction volumes at nanoliter levels, creating substantial savings in the amount of reagents and samples needed to perform testing.</p></div><div class="wp-block-emagine-column em-col"><div class="wp-block-image size-full"><div><figure class=""><noscript><img decoding="async" width="627" height="532" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5.png" alt="" class="wp-image-4292" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5.png 627w, https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5-300x255.png 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5-100x85.png 100w" sizes="(max-width: 627px) 100vw, 627px" /></noscript><img decoding="async" width="627" height="532" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%20627%20532%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5.png" alt="" class="lazyload wp-image-4292" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5.png 627w, https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5-300x255.png 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/image1-5-100x85.png 100w" data-sizes="(max-width: 627px) 100vw, 627px" /></figure></div></div></div></div></div><div class="wp-block-emagine-background alignfull has-gray-xxl-background-color has-background-dim em-pt--md em-pb--md em-mt--md em-mb--none"><div class="wp-block-emagine-background__content"><div class="wp-block-emagine-background__content-inner"><div class="wp-block-emagine-grid alignwide wp-block-emagine-grid--halves"><div class="wp-block-emagine-grid__inner em-grid-2_sm-1-left-middle"><div class="wp-block-emagine-column em-col-middle"><div class="wp-block-image size-full"><div><figure class=""><noscript><img decoding="async" width="943" height="799" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one.png" alt="" class="wp-image-4290" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one.png 943w, https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one-300x254.png 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one-768x651.png 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one-100x85.png 100w" sizes="(max-width: 943px) 100vw, 943px" /></noscript><img decoding="async" width="943" height="799" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%20943%20799%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one.png" alt="" class="lazyload wp-image-4290" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one.png 943w, https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one-300x254.png 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one-768x651.png 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/qx-one-100x85.png 100w" data-sizes="(max-width: 943px) 100vw, 943px" /></figure></div></div></div><div class="wp-block-emagine-column em-col-middle"><h3 class="h3"><strong>How the ddPCR platforms work</strong></h3><p class="">The QX200 and QX ONE ddPCR Systems from Bio-Rad are part of BioAgilytix’s extensive portfolio of PCR technologies and automation systems supporting our core genomic services. Our scientists typically turn to this modality to deliver precise and multiplexed PCR analysis for absolute quantification. As a world-class provider of bioanalysis, we often deal with complex assay demands, and the QX ONE ddPCR system platform enables us to effectively address our customers’ multiplex molecular challenges.</p><p class="">The QX ONE ddPCR System can deliver highly sensitive, and reproducible results. The system is fully automated and enables walk-away automation and ease of multiplexing. ddPCR allows for simultaneous target detection without the need for reference materials due to the precision derived from droplet generation methodology. Automation also eliminates pipetting error and leads to further reproducibility.</p></div></div></div></div></div></div><div id="block_d5b662c90a6b46b20596aa6a7d988ffe" class="wp-block em-call-to-action em-mt--none em-mb--none alignfull"><div class="em-call-to-action--align-center has-primary-l-background-color has-background-opacity-- em-pt--md em-pb--md em-pl--md em-pr--md"><div class="em-call-to-action__inner"><p class="">Publication</p><h2 class="h2">Best Practices in qPCR and dPCR Validation in Regulated Bioanalytical Laboratories</h2><div class="wp-block-emagine-button has-text-align-left"><a class="btn btn--size-md btn--style-primary has-quaternary-background-color" href="https://pubmed.ncbi.nlm.nih.gov/35194700/" target="_blank" rel="noreferrer noopener"><span>Learn <span class="btn__last-word">More</span></span></a></div></div></div></div></article></main><footer class="site-footer site-footer--has-logo site-footer--align-brand-left"><div class="deep-footer clearfix"><div 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