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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsysimage_f29 .image-contain" + "," + "#cq-image-jsp-"+"4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsysimage_f29 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><h2> Western blot to a new tune </h2></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>The Invitrogen Bandmate Automated Western Blot Processor is a programmable blot rocking system that frees up time by automating the tedious hands-on blocking, washing, and antibody incubation steps of western blot processing.</p></div></div><div container-id="cq-colctrl-lt8" comp-id="B3"><div class="text parbase section"><p><a href="/sg/en/home/global/forms/life-science/bandamate-automated-western-blot-processor-demo-request.html" class="btn btn-featured">Contact a technical specialist</a>  <a class="btn btn-primary" href="/sg/en/home/global/forms/life-science/bandamate-automated-western-blot-processor-demo-request.html">Request Demo</a></p></div></div></div></div><div style="clear:both"></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-b3641">Is it for me?</a></li><li><a data-toggle="tab" href="#container-b3642">How does it work?</a></li><li><a data-toggle="tab" href="#container-b3643">What does it offer?</a></li><li><a data-toggle="tab" href="#container-b3644">Documents</a></li><li><a data-toggle="tab" href="#container-b3647">Specifications</a></li><li><a data-toggle="tab" href="#container-b3645">Pricing</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-b3641" class="tab-pane active"><div class="container-b3641 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Automated western blot processing </h2></div><div class="text parbase section"><p>The western blot processing procedure is a hands-on, laborious process involving multiple rounds of washes and antibody incubations. With so many steps, its challenging to focus on other work or experiments while processing a western blot, especially if processing several blots at a time. Often, a specific protocol and reagents are optimized for a protein of interest. The Bandmate Automated Western Blot Processor requires no special reagents and is programmable to match your current western blotting protocols. Minimal effort is required to setup the Bandmate device to process up to four blots using your current reagents and protocols for blot processing, but with much less hands-on time (<b>Figure 1</b>). </p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3641tabtextimage_a883"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/_jcr_content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/backgroundimg.img.jpg/1686771979927.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.low.jpg/1686771979905.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.medium.jpg/1686771979905.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.480.medium.jpg/1686771979905.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.620.high.jpg/1686771979905.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.full.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.480.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.medium.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.620.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.low.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.480.medium.jpg/1686771979905.jpg" alt='The Bandmate Automated Western Blot Processor automates the multiple hands-on steps involved in manual western blot processing, freeing up time to reinvest in other important tasks and activities' title='The Bandmate Automated Western Blot Processor automates the multiple hands-on steps involved in manual western blot processing, freeing up time to reinvest in other important tasks and activities'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3641tabtextimage_a883 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3641tabtextimage_a883 img { width: 100%; max-width: 978px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"></span>Click image to enlarge</small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><span class="muted"><small><b>Figure 1. The Bandmate Automated Western Blot Processor automates the multiple hands-on steps involved in manual western blot processing, freeing up time to reinvest in other important tasks and activities.</b> Diagram compares example workflows for manual western blot processing (top) versus or automated processing (bottom) where timing of each step varies depending on the end-user protocol. The Bandmate Automated Western Blot processor can save approximately 20 minutes compared to manual processing. The time savings is associated with automation of multiple steps which prevents disruption at key points, thus allowing one to focus on other efforts during the processing protocol.</small></span></p><p><span class="muted"><small>* Note: Blocking buffer must be physically added to the Bandmate sample trays but is automatically drained from the trays before proceeding with the next automated primary antibody incubation step.</small></span></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-b3642" class="tab-pane"><div class="container-b3642 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> How the Bandmate system works </h2></div><div class="text parbase section"><p>Blocking buffer, wash buffer, and antibody solutions can be prepared as one would normally. Once the solutions are prepared, the wash buffer must be added to a wash buffer reservoir bottle which will dispense the required volumes of wash buffer throughout the processing procedure. Next, antibody solutions are loaded into Bandmate Reagent Tubes (minimum volume of 3.5 mL of primary and secondary antibody solutions for each mini blot or 7.5 mL for each midi blot). Then, the blot membranes are placed in individual sample trays, which are supported by rocker cradle frames. Blocking buffer is added directly to each tray. If antibody recovery is desired after incubation steps, antibody recovery tubes (50 mL conical tubes) can be inserted on each side of the waste trays to capture both primary and secondary antibody separately. Once the Bandmate device is setup with the necessary reagents for processing, pre-programmed protocols, stored custom protocols, or newly created custom protocols can be utilized. After the program is set, all processing steps are performed in a synchronized manner without further input needed – set up and walk away.</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3642tabtextimage_5c69"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.full.high.jpg/1686771979963.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.full.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.320.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.480.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.320.medium.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.620.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.320.low.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.480.medium.jpg/1686771979963.jpg" alt='Key components of the Bandmate Automated Western Blot processor' title='Key components of the Bandmate Automated Western Blot processor'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3642tabtextimage_5c69 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3642tabtextimage_5c69 img { width: 100%; max-width: 382px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b><span class="muted">Figure 2. Key components of the Bandmate Automated Western Blot processor: (1) reagent tubes (antibody reservoirs) (2) wash buffer tubing (3) rocker cradle (4) tray cover (5) sample tray (6) reagent slider (7) waste and recovery funnel tray (8) waste tank (compartment behind door panel).</span></b></small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>As the protocol proceeds, the sealed-bottom Bandmate Reagent Tubes are punctured to deliver antibody directly to the processing trays. The reagent tubes move in and out of place when necessary via the reagent slider bar. After incubation, antibody can be sent to waste or funneled into antibody recovery tubes (if recovering antibody for future experiments is desired) via the waste and recovery funnel tray. With this unique dispensing system, antibodies are not passing through any fixed tubing, minimizing potential cross-contamination.</p><p>The Bandmate processor provides a consistent rocking motion during incubation and wash steps. The rocking motion combined with the unique curved incubation trays helps minimize the required antibody volumes.</p><p>The Bandmate processor can be set up to run at room temperature or in a cold room overnight. For convenience, the final wash buffer is held in the processing tray and the rocking motion is maintained so blots remain saturated and ready for the detection step as soon as you are ready. The Bandmate processor comes with everything needed to process up to 4 mini blots or 2 midi blots at the same time. The only required consumables are the Bandmate Reagent Tubes which are changed with each processing experiment.</p><p>For more detailed information on the operation of Bandmate system, refer to the quick start user guide or user manual.</p><ul class="the-icons"><li><span class="icon-pdf"></span><b>User Guide:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018722_BandmateProcessor_UG.pdf" target="_blank">Bandmate Processor</a></li><li><span class="icon-pdf"></span><b>Manual:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018648_BandmateProcessor_QR.pdf" target="_blank">Bandmate processor</a></li></ul></div></div><div class="end"></div></div></div><div id="container-b3643" class="tab-pane"><div class="container-b3643 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Reduced hands-on-time </h2></div><div class="textimage parbase textimage-right section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_f614"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.full.high.jpg/1686771980054.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.full.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.320.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.480.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.320.medium.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.620.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.320.low.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.480.medium.jpg/1686771980054.jpg" alt='clock-yellowgreen' title='clock-yellowgreen'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_f614 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_f614 img { width: 100%; max-width: 99px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Free up valuable time in the lab to focus on other important activities and experiments. The programmable Bandmate system automatically performs the otherwise hands-on blocking, washing, and antibody incubations steps. Process 1-4 mini blots or 1-2 midi blots simultaneously to help maximize throughput.  Bandmate’s processing and collection trays are also easy to remove for quick clean-up.</p></div></div><div class="clearfix"></div></div><div class="heading section"><h2> No need to re-optimize conditions </h2></div><div class="text parbase section"><p>With simple and reliable operation, program the timing of incubation steps as you would normally, including adjustments for:</p><ul><li><b>Dispensing volumes </b>- up to 75 mL (for mini blots), and up to 150 mL (for midi blots); programmable with up to 10 repetitions per step </li><li><b>Rocking speeds</b> - from 5 to 40 cycles per minute</li><li><b>Incubation times</b> - from 1 minute to 96 hours for blots that may require overnight or longer incubations</li><li><b>Processing temperatures </b>- the system can be placed in a 4°C cold room if necessary—no special reagents are required</li></ul><p>The mini blots processed with the Bandmate Automated Western Blot Processor show comparable intensity levels across multiple probed targets compared to the blots processed with a manual processing procedure (Figure 3).</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_e499"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/_jcr_content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/backgroundimg.img.jpg/1686771980157.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.low.jpg/1686771980092.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.medium.jpg/1686771980092.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.480.medium.jpg/1686771980092.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.620.high.jpg/1686771980092.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.full.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.480.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.medium.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.620.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.low.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.480.medium.jpg/1686771980092.jpg" alt='Comparison of mini blots processed with Invitrogen Bandmate Automated Western Blot Processor vs. manual processing (probing and washing steps performed in a tray and on a shaker platform)' title='Comparison of mini blots processed with Invitrogen Bandmate Automated Western Blot Processor vs. manual processing (probing and washing steps performed in a tray and on a shaker platform)'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_e499 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_e499 img { width: 100%; max-width: 1000px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"></span>Click image to enlarge</small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 3. Comparison of mini blots processed with Invitrogen Bandmate Automated Western Blot Processor vs. manual processing (probing and washing steps performed in a tray and on a shaker platform). </b> A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/WXP42012BOX">Novex 4-20% Tris-Glycine gel</a> (Cat. No. WXP42012BOX), and electrophoresed at 225V for approximately 40 minutes.  Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (Cat. No. PB0012) and <a adhocenable="false" href="/order/catalog/product/PB7220">Power Blotter Pre-cut Membranes and Filters</a> (Cat. No. PB7220). One set of blots (4 total) were processed using the Bandmate Automated Western Blot Processor and was compared to another set of blots processed manually (2 total). Membranes were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37565">Blocker FL Fluorescent Blocking Buffer</a> (Cat. No. 37565).  For fluorescence multiplex detection, the blots were probed with a mixture of primary antibodies diluted in 1X Blocker FL Fluorescent Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/STAT3-Antibody-clone-9D8-Monoclonal/MA1-13042">Mouse Anti STAT3 (1:1000)</a> (Cat. No. MA1-13042), <a adhocenable="false" href="/antibody/product/Calreticulin-Antibody-Polyclonal/PA1-903">Chicken anti-Calreticulin (1:2000)</a> (Cat. No. PA1-903), and <a adhocenable="false" href="/antibody/product/Cyclophilin-B-Antibody-Polyclonal/PA1-027A">Rabbit Anti Cyclophilin B</a> (1:1000) (Cat. No. PA1-027A). This was followed by an incubation with secondary antibodies diluted at 1:5000 in 1X Blocker FL Fluorescent Blocking Buffer, specifically: <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32729">Goat anti-Mouse Alexa Fluor Plus 680</a> (Cat. No. A32729), <a adhocenable="false" href="/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11040">Goat anti Chicken Alexa Fluor 546</a> (Cat. No. A11040) and <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/SA5-35571">Goat anti Rabbit DyLight 800 4XPEG</a> (Cat. No. SA5-35571).  Membranes were imaged on the iBright FL1500 Imaging System. </span></small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>The midi blots processed with the Bandmate Automated Western Blot Processor show comparable intensity levels across multiple probed targets compared to the blots processed with a manual processing procedure (<b>Figure 4</b>).</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_af23"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.low.jpg/1686771980170.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.medium.jpg/1686771980170.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.480.medium.jpg/1686771980170.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.620.high.jpg/1686771980170.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.full.high.jpg/1686771980170.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.full.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.480.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.medium.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.620.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.low.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.480.medium.jpg/1686771980170.jpg" alt='Comparison of Midi blots processed with Bandmate Automated Western Blot Processor versus manual processing (probing and washing steps performed in a tray and on a shaker platform)' title='Comparison of Midi blots processed with Bandmate Automated Western Blot Processor versus manual processing (probing and washing steps performed in a tray and on a shaker platform)'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_af23 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_af23 img { width: 100%; max-width: 1117px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 4. Comparison of Midi blots processed with Bandmate Automated Western Blot Processor versus manual processing (probing and washing steps performed in a tray and on a shaker platform). </b>A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/WXP42020BOX">Novex 4-20% Tris-Glycine gel</a> (WXP42020BOX), and electrophoresed at 225V for approximately 36 minutes.  Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (Cat. No. PB0012) and <a adhocenable="false" href="/order/catalog/product/PB3210">Power Blotter Select Transfer Stacks</a> (Cat. No. PB3210). One set of blots (2 total) was processed using the Bandmate Automated Western Blot Processor and was compared another blot processed manually. Membranes were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37587">Clear Milk Blocking Buffer</a> (Cat. No. 37587).  Blots were probed with a mixture of primary antibodies diluted in 1X Clear Milk Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/KDEL-Antibody-Polyclonal/PA1-013">Rabbit Anti Hsp90 (1:5000)</a> (Cat. No. PA1-013) and <a adhocenable="false" href="/antibody/product/PDI-Antibody-clone-RL90-Monoclonal/MA3-019">Mouse Anti PDI (1:5000)</a> (Cat. No. MA3-019). This was followed by an incubation with the secondary antibodies, <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/32460">Stabilized Goat anti-Rabbit HRP 10 µg/mL (1:2500)</a> (Cat. No. 32460) and <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Secondary-Antibody-Polyclonal/32430">Stabilized Goat anti-Mouse HRP 10 µg/mL (1:2500)</a> (Cat. No. 32430). For chemiluminescence detection, blots were incubated with <a adhocenable="false" href="/order/catalog/product/34096">Supersignal West Femto Substrate</a> (Cat. No. 34096). Membranes were imaged on the iBright FL1500 Imaging System. </span></small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h2> Consistency day-to-day and person-to-person </h2></div><div class="text parbase section"><p>Maintaining the precise timing of block, wash, and antibody incubation steps can be challenging from blot-to-blot. Because the processing steps of the Bandmate system are programmable, the precision of the steps can be improved compared to manual hands-on western blot processing. With more precision, the consistency from experiment to experiment should also be improved for more reliable results.</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_dd7b"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.low.jpg/1686771980207.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.medium.jpg/1686771980207.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.480.medium.jpg/1686771980207.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.620.high.jpg/1686771980207.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.full.high.jpg/1686771980207.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.full.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.480.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.medium.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.620.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.low.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.480.medium.jpg/1686771980207.jpg" alt='The detection of proteins is consistent from blot-to-blot when blots are processed with the Bandmate Automated Western Blot Processor' title='The detection of proteins is consistent from blot-to-blot when blots are processed with the Bandmate Automated Western Blot Processor'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_dd7b { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_dd7b img { width: 100%; max-width: 1825px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 5. The detection of proteins is consistent from blot-to-blot when blots are processed with the Bandmate Automated Western Blot Processor.</b> A 2-fold dilution series of HeLa cell lysate starting at 1.0ug/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/WXP42012BOX">Novex 4-20% Tris-Glycine gel</a> (WXP42012BOX), and electrophoresed at 225V for approximately 40minutes.  Gels were transferred to nitrocellulose membranes using <a adhocenable="false" href="/order/catalog/product/IB23002">iBlot™ 2 Transfer Stacks</a> (Cat. No. IB23002). Four blots were processed using the Bandmate Automated Western Blot Processor following a preset protocol. The blots were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37565">Blocker FL Fluorescent Blocking Buffer</a> (Cat. No. 37565).  The blots were probed with a mixture of primary antibodies diluted in 1X Blocker FL Fluorescent Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/KDEL-Antibody-Polyclonal/PA1-013">Rabbit Anti Hsp90 (1:5000)</a> (Cat. No. PA1-013), <a adhocenable="false" href="/antibody/product/Calreticulin-Antibody-Polyclonal/PA1-903">Chicken anti-Calreticulin (1:4000)</a> (Cat. No. PA1-903) and <a adhocenable="false" href="/antibody/product/p23-Antibody-clone-JJ3-Monoclonal/MA3-414">Mouse Anti P23(1:1000)</a> (Cat. No. MA3-414). This was followed by an incubation with secondary antibodies diluted at 1:5000 in 1X Blocker FL: <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32729">Goat anti-Mouse Alexa Fluor Plus 680</a> (Cat. No. A32729), <a adhocenable="false" href="/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11040">Goat anti Chicken Alexa Fluor 546</a> (Cat. No. A11040) and <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/SA5-35571">Goat anti Rabbit DyLight 800 4XPEG</a> (Cat. No. SA5-35571).  Blots were imaged on the iBright FL1500 Imaging System.</span></small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h2> Helps save precious antibodies </h2></div><div class="text parbase section"><p>With the Bandmate system, one can use as little as 3.5 mL of primary and secondary antibody solutions for mini blots or 7.5 mL of primary and secondary antibody solutions for midi blots with minimal impact to sensitivity of detection (<b>Figure 6</b>).</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_ca93"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/_jcr_content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/backgroundimg.img.jpg/1686771980264.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.low.jpg/1686771980243.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.medium.jpg/1686771980243.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.480.medium.jpg/1686771980243.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.620.high.jpg/1686771980243.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.full.high.jpg/1686771980243.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.full.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.480.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.medium.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.620.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.low.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.480.medium.jpg/1686771980243.jpg" alt='The Bandmate Automated Western Blot Processor can be effective with as low as 3.5mL of primary antibody per mini blot or 7.5mL per midi blot without a significant loss in sensitivity of detection' title='The Bandmate Automated Western Blot Processor can be effective with as low as 3.5mL of primary antibody per mini blot or 7.5mL per midi blot without a significant loss in sensitivity of detection'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_ca93 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_ca93 img { width: 100%; max-width: 1441px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"></span>Click image to enlarge</small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 6. The Bandmate Automated Western Blot Processor can be effective with as low as 3.5mL of primary antibody per mini blot or 7.5 mL per midi blot without a significant loss in sensitivity of detection.</b> A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/mL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/NW04122BOX">Bolt 4-12% Bis-Tris-Plus gel</a> (NW04122BOX) for mini gels and <a adhocenable="false" href="/order/catalog/product/WG1401BOX">NuPAGE 4-12% Bis-Tris-Plus gel</a> (WG1401BOX) for midi gels, and electrophoresed at 200V for approximately 22 minutes. Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (Cat. No. PB0012) and <a adhocenable="false" href="/order/catalog/product/PB3210">Power Blotter Select Transfer Stacks</a> (Cat. No. PB3210). To test primary antibody volume efficiency using the Bandmate Automated Western Blot Processor, three different volumes of primary antibody were tested for mini blots (3.5 mL, 5 mL and 10 mL) and two volumes were tested for midi blots (7.5 mL and 15 mL). Blots were processed with adapted protocols for each volume. Blots were blocked in 1X <a adhocenable="false" href="https://thermofisher.com/order/catalog/product/37587">Clear Milk Blocking Buffer</a> (Cat. No. 37587).  Blots were probed with a mixture of primary antibodies diluted in 1X Clear Milk Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/STAT3-Antibody-clone-9D8-Monoclonal/MA1-13042">Mouse Anti STAT3 (1:500)</a> (Cat. No. MA1-13042), <a adhocenable="false" href="/antibody/product/PDI-Antibody-clone-RL90-Monoclonal/MA3-019">Mouse Anti PDI (1:2500)</a> (Cat. No. MA3-019), and <a adhocenable="false" href="/antibody/product/Cyclophilin-B-Antibody-Polyclonal/PA1-027A">Rabbit Anti Cyclophilin B (1:1000)</a> (Cat. No. PA1-027A). This was followed by an incubation with secondary antibody Stabilized <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/32460">Goat anti-Rabbit HRP 10 µg/mL (1:500)</a> (Cat. No. 32460) and Stabilized <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Secondary-Antibody-Polyclonal/32430">Goat anti-Mouse HRP 10 µg/mL (1:500)</a> (Cat. No. 32430). For chemiluminescence detection, blots were incubated with <a adhocenable="false" href="/order/catalog/product/34577">SuperSignal West Pico Plus Substrate</a> (Cat. No. 34577). Membranes were imaged on the iBright FL1500 Imaging System.</span></small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>With the Bandmate Automated Western Blot Processor, more than 90% of the starting antibody volume can be collected and recovered for future reuse. However, successive antibody reuses can lead to successive decreases in target detection and should be validated by the end-user (note: the impact on target detection as a result of successive antibody reuses can vary from antibody to antibody) (<b>Figure 7</b>). </p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_5c48"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.low.jpg/1686771980278.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.medium.jpg/1686771980278.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.480.medium.jpg/1686771980278.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.620.high.jpg/1686771980278.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.full.high.jpg/1686771980278.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.full.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.480.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.medium.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.620.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.low.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.480.medium.jpg/1686771980278.jpg" alt='The Bandmate system offers the ability to capture primary and secondary antibodies after the respective incubation steps, so the antibody solutions can be saved for reuse in future experiments' title='The Bandmate system offers the ability to capture primary and secondary antibodies after the respective incubation steps, so the antibody solutions can be saved for reuse in future experiments'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_5c48 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_5c48 img { width: 100%; max-width: 1184px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 7. The Bandmate system offers the ability to capture primary and secondary antibodies after the respective incubation steps, so the antibody solutions can be saved for reuse in future experiments.</b> A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/NW04122BOX">Bolt 4-12% Bis-Tris-Plus gel</a> (NW04122BOX), and electrophoresed at 200V for approximately 22 minutes.  Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (PB0012) and <a adhocenable="false" href="/order/catalog/product/PB3210">Power Blotter Select Transfer Stacks</a> (Cat. No. PB3210). To test the accuracy of antibody recovery and efficiency of detection, the Bandmate Automated Western Blot Processor protocol was set up to recover the primary antibody, and the same antibody was used three times. Blots were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37587">Clear Milk Blocking Buffer</a> (Cat. No. 37587), probed with <a adhocenable="false" href="/antibody/product/PDI-Antibody-clone-RL90-Monoclonal/MA3-019">Mouse Anti PDI (1:2500)</a> (Cat. No. MA3-019) diluted in 1X Clear Milk Blocking Buffer. This was followed by an incubation with secondary antibody <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32729">Goat anti-Mouse Alexa Fluor Plus 680</a> (Cat. No. A32729) diluted at 1:5000 in 1X TBST. Blots were imaged on the iBright FL1500 Imaging System.</span></small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-b3644" class="tab-pane"><div class="container-b3644 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><ul class="the-icons"><li><span class="icon-pdf"></span><b>User Guide:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018722_BandmateProcessor_UG.pdf" target="_blank">Bandmate Processor</a></li><li><span class="icon-pdf"></span><b>Manual:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018648_BandmateProcessor_QR.pdf" target="_blank">Bandmate processor</a></li></ul></div></div><div class="end"></div></div></div><div id="container-b3647" class="tab-pane"><div class="container-b3647 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Bandmate Automated Western Blot Processor specifications </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-939808318"><tbody><tr><td width="40%"><b>Capacity</b></td><td>Up to 4 mini blots or up to 2 midi blots</td></tr><tr><td><b>Rocks per minute</b></td><td>5—40 rpm</td></tr><tr><td><b>Maximum washes per step</b></td><td>10</td></tr><tr><td><b>Minimum reagent per blot</b></td><td>3.5 mL (Mini Blot), 7.5 mL (Midi Blot)</td></tr><tr><td><b>Maximum number of protocol steps</b></td><td>9</td></tr><tr><td><b>Maximum number of saved protocols</b></td><td>8 (6 custom and 2 default protocols)</td></tr><tr><td><b>Buffer dispensing volume</b></td><td>10—75 mL (mini blots), 10-150 mL (midi blots)</td></tr><tr><td><b>Incubation step time range</b></td><td>1 min to 96 hours</td></tr><tr><td><b>Operating temperature</b></td><td>4°C—40°C</td></tr><tr><td><b>Dimensions (L x W x H)</b></td><td>61 cm x 39 cm x 42 cm (24 in x 15.5 in x 16.5 in)</td></tr><tr><td><b>Weight</b></td><td>Approximately 25 kg (55 lbs)</td></tr><tr><td><b>Power requirements</b></td><td>12 VDC, 5 Amps</td></tr><tr><td><b>Relative humidity</b></td><td>5%—90% non-condensing</td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-b3645" class="tab-pane"><div class="container-b3645 tabstep"><div class="start"></div><div class="tab parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">BW1000, ZGEXSCBW1000, BW020X5, BW020X25, BW0060, BW0070, BW0030, BW0040, BW0050, BW0010</div><div id='ltajax-7267344487212061319'></div><script type="text/javascript">(function() {$('#ltajax-7267344487212061319').load('/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3645/tab/productlist_412e.ajax.html?skulist=');})(); </script></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> Is it for me? </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-b3641"><div class="container-b3641 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Automated western blot processing </h2></div><div class="text parbase section"><p>The western blot processing procedure is a hands-on, laborious process involving multiple rounds of washes and antibody incubations. With so many steps, its challenging to focus on other work or experiments while processing a western blot, especially if processing several blots at a time. Often, a specific protocol and reagents are optimized for a protein of interest. The Bandmate Automated Western Blot Processor requires no special reagents and is programmable to match your current western blotting protocols. Minimal effort is required to setup the Bandmate device to process up to four blots using your current reagents and protocols for blot processing, but with much less hands-on time (<b>Figure 1</b>). </p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3641tabtextimage_a883"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/_jcr_content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/backgroundimg.img.jpg/1686771979927.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.low.jpg/1686771979905.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.medium.jpg/1686771979905.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.480.medium.jpg/1686771979905.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.620.high.jpg/1686771979905.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.full.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.480.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.medium.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.620.high.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.320.low.jpg/1686771979905.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3641/tab/textimage_a883/image.img.480.medium.jpg/1686771979905.jpg" alt='The Bandmate Automated Western Blot Processor automates the multiple hands-on steps involved in manual western blot processing, freeing up time to reinvest in other important tasks and activities' title='The Bandmate Automated Western Blot Processor automates the multiple hands-on steps involved in manual western blot processing, freeing up time to reinvest in other important tasks and activities'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3641tabtextimage_a883 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3641tabtextimage_a883 img { width: 100%; max-width: 978px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"></span>Click image to enlarge</small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><span class="muted"><small><b>Figure 1. The Bandmate Automated Western Blot Processor automates the multiple hands-on steps involved in manual western blot processing, freeing up time to reinvest in other important tasks and activities.</b> Diagram compares example workflows for manual western blot processing (top) versus or automated processing (bottom) where timing of each step varies depending on the end-user protocol. The Bandmate Automated Western Blot processor can save approximately 20 minutes compared to manual processing. The time savings is associated with automation of multiple steps which prevents disruption at key points, thus allowing one to focus on other efforts during the processing protocol.</small></span></p><p><span class="muted"><small>* Note: Blocking buffer must be physically added to the Bandmate sample trays but is automatically drained from the trays before proceeding with the next automated primary antibody incubation step.</small></span></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> How does it work? </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-b3642"><div class="container-b3642 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> How the Bandmate system works </h2></div><div class="text parbase section"><p>Blocking buffer, wash buffer, and antibody solutions can be prepared as one would normally. Once the solutions are prepared, the wash buffer must be added to a wash buffer reservoir bottle which will dispense the required volumes of wash buffer throughout the processing procedure. Next, antibody solutions are loaded into Bandmate Reagent Tubes (minimum volume of 3.5 mL of primary and secondary antibody solutions for each mini blot or 7.5 mL for each midi blot). Then, the blot membranes are placed in individual sample trays, which are supported by rocker cradle frames. Blocking buffer is added directly to each tray. If antibody recovery is desired after incubation steps, antibody recovery tubes (50 mL conical tubes) can be inserted on each side of the waste trays to capture both primary and secondary antibody separately. Once the Bandmate device is setup with the necessary reagents for processing, pre-programmed protocols, stored custom protocols, or newly created custom protocols can be utilized. After the program is set, all processing steps are performed in a synchronized manner without further input needed – set up and walk away.</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3642tabtextimage_5c69"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.full.high.jpg/1686771979963.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.full.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.320.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.480.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.320.medium.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.620.high.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.320.low.jpg/1686771979963.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3642/tab/textimage_5c69/image.img.480.medium.jpg/1686771979963.jpg" alt='Key components of the Bandmate Automated Western Blot processor' title='Key components of the Bandmate Automated Western Blot processor'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3642tabtextimage_5c69 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3642tabtextimage_5c69 img { width: 100%; max-width: 382px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b><span class="muted">Figure 2. Key components of the Bandmate Automated Western Blot processor: (1) reagent tubes (antibody reservoirs) (2) wash buffer tubing (3) rocker cradle (4) tray cover (5) sample tray (6) reagent slider (7) waste and recovery funnel tray (8) waste tank (compartment behind door panel).</span></b></small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>As the protocol proceeds, the sealed-bottom Bandmate Reagent Tubes are punctured to deliver antibody directly to the processing trays. The reagent tubes move in and out of place when necessary via the reagent slider bar. After incubation, antibody can be sent to waste or funneled into antibody recovery tubes (if recovering antibody for future experiments is desired) via the waste and recovery funnel tray. With this unique dispensing system, antibodies are not passing through any fixed tubing, minimizing potential cross-contamination.</p><p>The Bandmate processor provides a consistent rocking motion during incubation and wash steps. The rocking motion combined with the unique curved incubation trays helps minimize the required antibody volumes.</p><p>The Bandmate processor can be set up to run at room temperature or in a cold room overnight. For convenience, the final wash buffer is held in the processing tray and the rocking motion is maintained so blots remain saturated and ready for the detection step as soon as you are ready. The Bandmate processor comes with everything needed to process up to 4 mini blots or 2 midi blots at the same time. The only required consumables are the Bandmate Reagent Tubes which are changed with each processing experiment.</p><p>For more detailed information on the operation of Bandmate system, refer to the quick start user guide or user manual.</p><ul class="the-icons"><li><span class="icon-pdf"></span><b>User Guide:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018722_BandmateProcessor_UG.pdf" target="_blank">Bandmate Processor</a></li><li><span class="icon-pdf"></span><b>Manual:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018648_BandmateProcessor_QR.pdf" target="_blank">Bandmate processor</a></li></ul></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> What does it offer? </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-b3643"><div class="container-b3643 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Reduced hands-on-time </h2></div><div class="textimage parbase textimage-right section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_f614"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.full.high.jpg/1686771980054.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.full.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.320.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.480.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.320.medium.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.620.high.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.320.low.jpg/1686771980054.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_f614/image.img.480.medium.jpg/1686771980054.jpg" alt='clock-yellowgreen' title='clock-yellowgreen'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_f614 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_f614 img { width: 100%; max-width: 99px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Free up valuable time in the lab to focus on other important activities and experiments. The programmable Bandmate system automatically performs the otherwise hands-on blocking, washing, and antibody incubations steps. Process 1-4 mini blots or 1-2 midi blots simultaneously to help maximize throughput.  Bandmate’s processing and collection trays are also easy to remove for quick clean-up.</p></div></div><div class="clearfix"></div></div><div class="heading section"><h2> No need to re-optimize conditions </h2></div><div class="text parbase section"><p>With simple and reliable operation, program the timing of incubation steps as you would normally, including adjustments for:</p><ul><li><b>Dispensing volumes </b>- up to 75 mL (for mini blots), and up to 150 mL (for midi blots); programmable with up to 10 repetitions per step </li><li><b>Rocking speeds</b> - from 5 to 40 cycles per minute</li><li><b>Incubation times</b> - from 1 minute to 96 hours for blots that may require overnight or longer incubations</li><li><b>Processing temperatures </b>- the system can be placed in a 4°C cold room if necessary—no special reagents are required</li></ul><p>The mini blots processed with the Bandmate Automated Western Blot Processor show comparable intensity levels across multiple probed targets compared to the blots processed with a manual processing procedure (Figure 3).</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_e499"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/_jcr_content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/backgroundimg.img.jpg/1686771980157.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.low.jpg/1686771980092.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.medium.jpg/1686771980092.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.480.medium.jpg/1686771980092.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.620.high.jpg/1686771980092.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.full.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.480.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.medium.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.620.high.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.320.low.jpg/1686771980092.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_e499/image.img.480.medium.jpg/1686771980092.jpg" alt='Comparison of mini blots processed with Invitrogen Bandmate Automated Western Blot Processor vs. manual processing (probing and washing steps performed in a tray and on a shaker platform)' title='Comparison of mini blots processed with Invitrogen Bandmate Automated Western Blot Processor vs. manual processing (probing and washing steps performed in a tray and on a shaker platform)'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_e499 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_e499 img { width: 100%; max-width: 1000px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"></span>Click image to enlarge</small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 3. Comparison of mini blots processed with Invitrogen Bandmate Automated Western Blot Processor vs. manual processing (probing and washing steps performed in a tray and on a shaker platform). </b> A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/WXP42012BOX">Novex 4-20% Tris-Glycine gel</a> (Cat. No. WXP42012BOX), and electrophoresed at 225V for approximately 40 minutes.  Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (Cat. No. PB0012) and <a adhocenable="false" href="/order/catalog/product/PB7220">Power Blotter Pre-cut Membranes and Filters</a> (Cat. No. PB7220). One set of blots (4 total) were processed using the Bandmate Automated Western Blot Processor and was compared to another set of blots processed manually (2 total). Membranes were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37565">Blocker FL Fluorescent Blocking Buffer</a> (Cat. No. 37565).  For fluorescence multiplex detection, the blots were probed with a mixture of primary antibodies diluted in 1X Blocker FL Fluorescent Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/STAT3-Antibody-clone-9D8-Monoclonal/MA1-13042">Mouse Anti STAT3 (1:1000)</a> (Cat. No. MA1-13042), <a adhocenable="false" href="/antibody/product/Calreticulin-Antibody-Polyclonal/PA1-903">Chicken anti-Calreticulin (1:2000)</a> (Cat. No. PA1-903), and <a adhocenable="false" href="/antibody/product/Cyclophilin-B-Antibody-Polyclonal/PA1-027A">Rabbit Anti Cyclophilin B</a> (1:1000) (Cat. No. PA1-027A). This was followed by an incubation with secondary antibodies diluted at 1:5000 in 1X Blocker FL Fluorescent Blocking Buffer, specifically: <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32729">Goat anti-Mouse Alexa Fluor Plus 680</a> (Cat. No. A32729), <a adhocenable="false" href="/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11040">Goat anti Chicken Alexa Fluor 546</a> (Cat. No. A11040) and <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/SA5-35571">Goat anti Rabbit DyLight 800 4XPEG</a> (Cat. No. SA5-35571).  Membranes were imaged on the iBright FL1500 Imaging System. </span></small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>The midi blots processed with the Bandmate Automated Western Blot Processor show comparable intensity levels across multiple probed targets compared to the blots processed with a manual processing procedure (<b>Figure 4</b>).</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_af23"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.low.jpg/1686771980170.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.medium.jpg/1686771980170.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.480.medium.jpg/1686771980170.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.620.high.jpg/1686771980170.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.full.high.jpg/1686771980170.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.full.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.480.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.medium.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.620.high.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.320.low.jpg/1686771980170.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_af23/image.img.480.medium.jpg/1686771980170.jpg" alt='Comparison of Midi blots processed with Bandmate Automated Western Blot Processor versus manual processing (probing and washing steps performed in a tray and on a shaker platform)' title='Comparison of Midi blots processed with Bandmate Automated Western Blot Processor versus manual processing (probing and washing steps performed in a tray and on a shaker platform)'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_af23 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_af23 img { width: 100%; max-width: 1117px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 4. Comparison of Midi blots processed with Bandmate Automated Western Blot Processor versus manual processing (probing and washing steps performed in a tray and on a shaker platform). </b>A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/WXP42020BOX">Novex 4-20% Tris-Glycine gel</a> (WXP42020BOX), and electrophoresed at 225V for approximately 36 minutes.  Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (Cat. No. PB0012) and <a adhocenable="false" href="/order/catalog/product/PB3210">Power Blotter Select Transfer Stacks</a> (Cat. No. PB3210). One set of blots (2 total) was processed using the Bandmate Automated Western Blot Processor and was compared another blot processed manually. Membranes were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37587">Clear Milk Blocking Buffer</a> (Cat. No. 37587).  Blots were probed with a mixture of primary antibodies diluted in 1X Clear Milk Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/KDEL-Antibody-Polyclonal/PA1-013">Rabbit Anti Hsp90 (1:5000)</a> (Cat. No. PA1-013) and <a adhocenable="false" href="/antibody/product/PDI-Antibody-clone-RL90-Monoclonal/MA3-019">Mouse Anti PDI (1:5000)</a> (Cat. No. MA3-019). This was followed by an incubation with the secondary antibodies, <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/32460">Stabilized Goat anti-Rabbit HRP 10 µg/mL (1:2500)</a> (Cat. No. 32460) and <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Secondary-Antibody-Polyclonal/32430">Stabilized Goat anti-Mouse HRP 10 µg/mL (1:2500)</a> (Cat. No. 32430). For chemiluminescence detection, blots were incubated with <a adhocenable="false" href="/order/catalog/product/34096">Supersignal West Femto Substrate</a> (Cat. No. 34096). Membranes were imaged on the iBright FL1500 Imaging System. </span></small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h2> Consistency day-to-day and person-to-person </h2></div><div class="text parbase section"><p>Maintaining the precise timing of block, wash, and antibody incubation steps can be challenging from blot-to-blot. Because the processing steps of the Bandmate system are programmable, the precision of the steps can be improved compared to manual hands-on western blot processing. With more precision, the consistency from experiment to experiment should also be improved for more reliable results.</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_dd7b"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.low.jpg/1686771980207.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.medium.jpg/1686771980207.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.480.medium.jpg/1686771980207.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.620.high.jpg/1686771980207.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.full.high.jpg/1686771980207.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.full.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.480.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.medium.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.620.high.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.320.low.jpg/1686771980207.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_dd7b/image.img.480.medium.jpg/1686771980207.jpg" alt='The detection of proteins is consistent from blot-to-blot when blots are processed with the Bandmate Automated Western Blot Processor' title='The detection of proteins is consistent from blot-to-blot when blots are processed with the Bandmate Automated Western Blot Processor'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_dd7b { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_dd7b img { width: 100%; max-width: 1825px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 5. The detection of proteins is consistent from blot-to-blot when blots are processed with the Bandmate Automated Western Blot Processor.</b> A 2-fold dilution series of HeLa cell lysate starting at 1.0ug/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/WXP42012BOX">Novex 4-20% Tris-Glycine gel</a> (WXP42012BOX), and electrophoresed at 225V for approximately 40minutes.  Gels were transferred to nitrocellulose membranes using <a adhocenable="false" href="/order/catalog/product/IB23002">iBlot™ 2 Transfer Stacks</a> (Cat. No. IB23002). Four blots were processed using the Bandmate Automated Western Blot Processor following a preset protocol. The blots were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37565">Blocker FL Fluorescent Blocking Buffer</a> (Cat. No. 37565).  The blots were probed with a mixture of primary antibodies diluted in 1X Blocker FL Fluorescent Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/KDEL-Antibody-Polyclonal/PA1-013">Rabbit Anti Hsp90 (1:5000)</a> (Cat. No. PA1-013), <a adhocenable="false" href="/antibody/product/Calreticulin-Antibody-Polyclonal/PA1-903">Chicken anti-Calreticulin (1:4000)</a> (Cat. No. PA1-903) and <a adhocenable="false" href="/antibody/product/p23-Antibody-clone-JJ3-Monoclonal/MA3-414">Mouse Anti P23(1:1000)</a> (Cat. No. MA3-414). This was followed by an incubation with secondary antibodies diluted at 1:5000 in 1X Blocker FL: <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32729">Goat anti-Mouse Alexa Fluor Plus 680</a> (Cat. No. A32729), <a adhocenable="false" href="/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11040">Goat anti Chicken Alexa Fluor 546</a> (Cat. No. A11040) and <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/SA5-35571">Goat anti Rabbit DyLight 800 4XPEG</a> (Cat. No. SA5-35571).  Blots were imaged on the iBright FL1500 Imaging System.</span></small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h2> Helps save precious antibodies </h2></div><div class="text parbase section"><p>With the Bandmate system, one can use as little as 3.5 mL of primary and secondary antibody solutions for mini blots or 7.5 mL of primary and secondary antibody solutions for midi blots with minimal impact to sensitivity of detection (<b>Figure 6</b>).</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_ca93"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/_jcr_content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/backgroundimg.img.jpg/1686771980264.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.low.jpg/1686771980243.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.medium.jpg/1686771980243.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.480.medium.jpg/1686771980243.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.620.high.jpg/1686771980243.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.full.high.jpg/1686771980243.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.full.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.480.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.medium.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.620.high.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.320.low.jpg/1686771980243.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_ca93/image.img.480.medium.jpg/1686771980243.jpg" alt='The Bandmate Automated Western Blot Processor can be effective with as low as 3.5mL of primary antibody per mini blot or 7.5mL per midi blot without a significant loss in sensitivity of detection' title='The Bandmate Automated Western Blot Processor can be effective with as low as 3.5mL of primary antibody per mini blot or 7.5mL per midi blot without a significant loss in sensitivity of detection'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_ca93 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_ca93 img { width: 100%; max-width: 1441px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"></span>Click image to enlarge</small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 6. The Bandmate Automated Western Blot Processor can be effective with as low as 3.5mL of primary antibody per mini blot or 7.5 mL per midi blot without a significant loss in sensitivity of detection.</b> A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/mL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/NW04122BOX">Bolt 4-12% Bis-Tris-Plus gel</a> (NW04122BOX) for mini gels and <a adhocenable="false" href="/order/catalog/product/WG1401BOX">NuPAGE 4-12% Bis-Tris-Plus gel</a> (WG1401BOX) for midi gels, and electrophoresed at 200V for approximately 22 minutes. Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (Cat. No. PB0012) and <a adhocenable="false" href="/order/catalog/product/PB3210">Power Blotter Select Transfer Stacks</a> (Cat. No. PB3210). To test primary antibody volume efficiency using the Bandmate Automated Western Blot Processor, three different volumes of primary antibody were tested for mini blots (3.5 mL, 5 mL and 10 mL) and two volumes were tested for midi blots (7.5 mL and 15 mL). Blots were processed with adapted protocols for each volume. Blots were blocked in 1X <a adhocenable="false" href="https://thermofisher.com/order/catalog/product/37587">Clear Milk Blocking Buffer</a> (Cat. No. 37587).  Blots were probed with a mixture of primary antibodies diluted in 1X Clear Milk Blocking Buffer at the following concentrations: <a adhocenable="false" href="/antibody/product/STAT3-Antibody-clone-9D8-Monoclonal/MA1-13042">Mouse Anti STAT3 (1:500)</a> (Cat. No. MA1-13042), <a adhocenable="false" href="/antibody/product/PDI-Antibody-clone-RL90-Monoclonal/MA3-019">Mouse Anti PDI (1:2500)</a> (Cat. No. MA3-019), and <a adhocenable="false" href="/antibody/product/Cyclophilin-B-Antibody-Polyclonal/PA1-027A">Rabbit Anti Cyclophilin B (1:1000)</a> (Cat. No. PA1-027A). This was followed by an incubation with secondary antibody Stabilized <a adhocenable="false" href="/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/32460">Goat anti-Rabbit HRP 10 µg/mL (1:500)</a> (Cat. No. 32460) and Stabilized <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Secondary-Antibody-Polyclonal/32430">Goat anti-Mouse HRP 10 µg/mL (1:500)</a> (Cat. No. 32430). For chemiluminescence detection, blots were incubated with <a adhocenable="false" href="/order/catalog/product/34577">SuperSignal West Pico Plus Substrate</a> (Cat. No. 34577). Membranes were imaged on the iBright FL1500 Imaging System.</span></small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>With the Bandmate Automated Western Blot Processor, more than 90% of the starting antibody volume can be collected and recovered for future reuse. However, successive antibody reuses can lead to successive decreases in target detection and should be validated by the end-user (note: the impact on target detection as a result of successive antibody reuses can vary from antibody to antibody) (<b>Figure 7</b>). </p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_5c48"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.low.jpg/1686771980278.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.medium.jpg/1686771980278.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.480.medium.jpg/1686771980278.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.620.high.jpg/1686771980278.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.full.high.jpg/1686771980278.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.full.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.480.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.medium.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.620.high.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.320.low.jpg/1686771980278.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3643/tab/textimage_5c48/image.img.480.medium.jpg/1686771980278.jpg" alt='The Bandmate system offers the ability to capture primary and secondary antibodies after the respective incubation steps, so the antibody solutions can be saved for reuse in future experiments' title='The Bandmate system offers the ability to capture primary and secondary antibodies after the respective incubation steps, so the antibody solutions can be saved for reuse in future experiments'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_5c48 { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainercontainer-b3643tabtextimage_5c48 img { width: 100%; max-width: 1184px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><span class="muted"><b>Figure 7. The Bandmate system offers the ability to capture primary and secondary antibodies after the respective incubation steps, so the antibody solutions can be saved for reuse in future experiments.</b> A 2-fold dilution series of HeLa cell lysate starting at 4.0 µg/µL was prepared, loaded onto a <a adhocenable="false" href="/order/catalog/product/NW04122BOX">Bolt 4-12% Bis-Tris-Plus gel</a> (NW04122BOX), and electrophoresed at 200V for approximately 22 minutes.  Gels were transferred to nitrocellulose membranes using the <a adhocenable="false" href="/order/catalog/product/PB0012">Power Blotter System</a> (PB0012) and <a adhocenable="false" href="/order/catalog/product/PB3210">Power Blotter Select Transfer Stacks</a> (Cat. No. PB3210). To test the accuracy of antibody recovery and efficiency of detection, the Bandmate Automated Western Blot Processor protocol was set up to recover the primary antibody, and the same antibody was used three times. Blots were blocked in 1X <a adhocenable="false" href="/order/catalog/product/37587">Clear Milk Blocking Buffer</a> (Cat. No. 37587), probed with <a adhocenable="false" href="/antibody/product/PDI-Antibody-clone-RL90-Monoclonal/MA3-019">Mouse Anti PDI (1:2500)</a> (Cat. No. MA3-019) diluted in 1X Clear Milk Blocking Buffer. This was followed by an incubation with secondary antibody <a adhocenable="false" href="/antibody/product/Goat-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32729">Goat anti-Mouse Alexa Fluor Plus 680</a> (Cat. No. A32729) diluted at 1:5000 in 1X TBST. Blots were imaged on the iBright FL1500 Imaging System.</span></small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer" href="#collapse4"><span class="caret"></span><strong> Documents </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-b3644"><div class="container-b3644 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><ul class="the-icons"><li><span class="icon-pdf"></span><b>User Guide:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018722_BandmateProcessor_UG.pdf" target="_blank">Bandmate Processor</a></li><li><span class="icon-pdf"></span><b>Manual:</b><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018648_BandmateProcessor_QR.pdf" target="_blank">Bandmate processor</a></li></ul></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer" href="#collapse5"><span class="caret"></span><strong> Specifications </strong></a></div><div id="collapse5" class="accordion-body collapse "><div class="accordion-inner container-b3647"><div class="container-b3647 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Bandmate Automated Western Blot Processor specifications </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-2129718695"><tbody><tr><td width="40%"><b>Capacity</b></td><td>Up to 4 mini blots or up to 2 midi blots</td></tr><tr><td><b>Rocks per minute</b></td><td>5—40 rpm</td></tr><tr><td><b>Maximum washes per step</b></td><td>10</td></tr><tr><td><b>Minimum reagent per blot</b></td><td>3.5 mL (Mini Blot), 7.5 mL (Midi Blot)</td></tr><tr><td><b>Maximum number of protocol steps</b></td><td>9</td></tr><tr><td><b>Maximum number of saved protocols</b></td><td>8 (6 custom and 2 default protocols)</td></tr><tr><td><b>Buffer dispensing volume</b></td><td>10—75 mL (mini blots), 10-150 mL (midi blots)</td></tr><tr><td><b>Incubation step time range</b></td><td>1 min to 96 hours</td></tr><tr><td><b>Operating temperature</b></td><td>4°C—40°C</td></tr><tr><td><b>Dimensions (L x W x H)</b></td><td>61 cm x 39 cm x 42 cm (24 in x 15.5 in x 16.5 in)</td></tr><tr><td><b>Weight</b></td><td>Approximately 25 kg (55 lbs)</td></tr><tr><td><b>Power requirements</b></td><td>12 VDC, 5 Amps</td></tr><tr><td><b>Relative humidity</b></td><td>5%—90% non-condensing</td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsystabworkflowcontainer" href="#collapse6"><span class="caret"></span><strong> Pricing </strong></a></div><div id="collapse6" class="accordion-body collapse "><div class="accordion-inner container-b3645"><div class="container-b3645 tabstep"><div class="start"></div><div class="tab parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">BW1000, ZGEXSCBW1000, BW020X5, BW020X25, BW0060, BW0070, BW0030, BW0040, BW0050, BW0010</div><div id='ltajax-4756146419156474312'></div><script type="text/javascript">(function() {$('#ltajax-4756146419156474312').load('/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/tabworkflowcontainer/container-b3645/tab/productlist_412e.ajax.html?skulist=');})(); </script></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="video"><br>How to process western blots with the Invitrogen Bandmate Automated Western Blot Processor </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="e7969a8accd8"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); 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</script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to process western blots with the Invitrogen Bandmate Automated Western Blot Processor", "description": "Learn how to process western blots efficiently with the Invitrogen Bandmate Automated Western Blot Processor", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/03dc1eec-f304-469d-8bb0-c19bba7a83f4/b36e3e8f-e1d3-4142-b4ac-f789e3811891/160x90/match/image.jpg"], "uploadDate": "2021-03-13T07:09:28.977Z", "duration": "PT6M58.027S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6239904241001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6239904241001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6239904241001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. 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} #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_72e6 img { width: 100%; max-width: 150px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Explore: <a href="/sg/en/home/life-science/antibodies.html">Western Blot Validated Antibodies</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c1"><div container-id="cq-colctrl-lt4" comp-id="B1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_2b9c"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.full.high.jpg/1686771980470.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.full.high.jpg/1686771980470.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.320.high.jpg/1686771980470.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.480.high.jpg/1686771980470.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.320.medium.jpg/1686771980470.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.620.high.jpg/1686771980470.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.320.low.jpg/1686771980470.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_2b9c/image.img.480.medium.jpg/1686771980470.jpg" alt='Western Blot Validated Antibodies ' title='Western Blot Validated Antibodies '></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_2b9c { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_2b9c img { width: 100%; max-width: 150px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Explore: <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels.html">Protein Gels</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c2"><div container-id="cq-colctrl-lt4" comp-id="C1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_e60e"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/western-workflow-overview-brochure.pdf" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.full.high.jpg/1686771980503.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.full.high.jpg/1686771980503.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.320.high.jpg/1686771980503.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.480.high.jpg/1686771980503.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.320.medium.jpg/1686771980503.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.620.high.jpg/1686771980503.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.320.low.jpg/1686771980503.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_e60e/image.img.480.medium.jpg/1686771980503.jpg" alt='iWestern Workflow—start-to-finish western blot solution' title='iWestern Workflow—start-to-finish western blot solution'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_e60e { max-width: 100%; } #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_e60e img { width: 100%; max-width: 150px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><a adhocenable="false" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/iwestern-workflow.html">iWestern Workflow—start-to-finish western blot solution</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c3"><div container-id="cq-colctrl-lt4" comp-id="D1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_7a2"><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-gel-electrophoresis-information.html" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.full.high.jpg/1686771980565.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.full.high.jpg/1686771980565.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.320.high.jpg/1686771980565.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.480.high.jpg/1686771980565.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.320.medium.jpg/1686771980565.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.620.high.jpg/1686771980565.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.320.low.jpg/1686771980565.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/bandmate-automated-processor/jcr:content/MainParsys/well_18d8/uipar/textimage_7a2/image.img.480.medium.jpg/1686771980565.jpg" alt='Western Detection Workflow Brochure' title='Western Detection Workflow Brochure'></picture></div><style> #cq-textimage-jsp-4ba62e36-fc39-4703-9aff-a9c7d7e41f25MainParsyswell_18d8uipartextimage_7a2 { max-width: 100%; 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