CINXE.COM
TMTcalibrator
<!doctype html> <html lang="en"> <head> <meta charset="utf-8"> <link rel="dns-prefetch preconnect" href="//www.google-analytics.com"> <title>TMTcalibrator</title> <meta name="viewport" content="width=device-width, initial-scale=1"> <meta name="description" content=""> <link type="text/css" rel="stylesheet" href="https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/css/main.1654711795.css"> <link rel="stylesheet" type="text/css" href="https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/tarteaucitron/css/tarteaucitron.1654711795.css"> <style> .grecaptcha-badge { visibility: hidden; } </style> <script>loadjs=function(){function e(e,n){var t,r,i,c=[],o=(e=e.push?e:[e]).length,f=o;for(t=function(e,t){t.length&&c.push(e),--f||n(c)};o--;)r=e[o],(i=s[r])?t(r,i):(u[r]=u[r]||[]).push(t)}function n(e,n){if(e){var t=u[e];if(s[e]=n,t)for(;t.length;)t[0](e,n),t.splice(0,1)}}function t(e,n,r,i){var o,s,u=document,f=r.async,a=(r.numRetries||0)+1,h=r.before||c;i=i||0,/(^css!|\.css$)/.test(e)?(o=!0,(s=u.createElement("link")).rel="stylesheet",s.href=e.replace(/^css!/,"")):((s=u.createElement("script")).src=e,s.async=void 0===f||f),s.onload=s.onerror=s.onbeforeload=function(c){var u=c.type[0];if(o&&"hideFocus"in s)try{s.sheet.cssText.length||(u="e")}catch(e){u="e"}if("e"==u&&(i+=1)<a)return t(e,n,r,i);n(e,u,c.defaultPrevented)},!1!==h(e,s)&&u.head.appendChild(s)}function r(e,n,r){var i,c,o=(e=e.push?e:[e]).length,s=o,u=[];for(i=function(e,t,r){if("e"==t&&u.push(e),"b"==t){if(!r)return;u.push(e)}--o||n(u)},c=0;c<s;c++)t(e[c],i,r)}function i(e,t,i){var s,u;if(t&&t.trim&&(s=t),u=(s?i:t)||{},s){if(s in o)throw"LoadJS";o[s]=!0}r(e,function(e){e.length?(u.error||c)(e):(u.success||c)(),n(s,e)},u)}var c=function(){},o={},s={},u={};return i.ready=function(n,t){return e(n,function(e){e.length?(t.error||c)(e):(t.success||c)()}),i},i.done=function(e){n(e,[])},i.reset=function(){o={},s={},u={}},i.isDefined=function(e){return e in o},i}();</script> <script>loadjs(['https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/js/main.1654711795.js'], 'main');</script> <script src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/tarteaucitron/tarteaucitron.1654711795.js"></script> <script> tarteaucitronCustomText = { "middleBarHead": "Cookies" }; tarteaucitron.init({ "tarteaucitronForceCDN": "https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/tarteaucitron", /* Force usage of our CDN */ "privacyUrl": "https://www.proteomics.com/privacy-notice", /* Privacy policy url */ "bodyPosition": "bottom", /* or top to bring it as first element for accessibility */ "hashtag": "#tarteaucitron", /* Open the panel with this hashtag */ "cookieName": "tarteaucitron", /* Cookie name */ "orientation": "popup", /* Banner position (top - bottom - middle - popup) */ "groupServices": false, /* Group services by category */ "showAlertSmall": false, /* Show the small banner on bottom right */ "cookieslist": false, /* Show the cookie list */ "showIcon": false, /* Show cookie icon to manage cookies */ "adblocker": false, /* Show a Warning if an adblocker is detected */ "DenyAllCta" : true, /* Show the deny all button */ "AcceptAllCta" : true, /* Show the accept all button when highPrivacy on */ "highPrivacy": true, /* HIGHLY RECOMMANDED Disable auto consent */ "handleBrowserDNTRequest": false, /* If Do Not Track == 1, disallow all */ "removeCredit": true, /* Remove credit link */ "moreInfoLink": false, /* Show more info link */ "useExternalCss": false, /* If false, the tarteaucitron.css file will be loaded */ "useExternalJs": false, /* If false, the tarteaucitron.services.js file will be loaded */ "mandatory": true, /* Show a message about mandatory cookies */ "mandatoryCta": false /* Show the disabled accept button when mandatory on */ }); </script> <script type="text/javascript"> tarteaucitron.user.analyticsUa = 'UA-25428798-1'; tarteaucitron.user.analyticsMore = function () { /* optionnal ga.push() */ }; tarteaucitron.user.analyticsUaCreate = { /* optionnal create configuration */ }; tarteaucitron.user.analyticsAnonymizeIp = true; tarteaucitron.user.analyticsPageView = { /* optionnal pageview configuration */ }; tarteaucitron.user.analyticsMore = function () { /* optionnal ga.push() */ }; (tarteaucitron.job = tarteaucitron.job || []).push('analytics'); </script> <script>!function(t){"use strict";t.loadCSS||(t.loadCSS=function(){});var e=loadCSS.relpreload={};if(e.support=function(){var e;try{e=t.document.createElement("link").relList.supports("preload")}catch(t){e=!1}return function(){return e}}(),e.bindMediaToggle=function(t){function e(){t.media=a}var a=t.media||"all";t.addEventListener?t.addEventListener("load",e):t.attachEvent&&t.attachEvent("onload",e),setTimeout(function(){t.rel="stylesheet",t.media="only x"}),setTimeout(e,3e3)},e.poly=function(){if(!e.support())for(var a=t.document.getElementsByTagName("link"),n=0;n<a.length;n++){var o=a[n];"preload"!==o.rel||"style"!==o.getAttribute("as")||o.getAttribute("data-loadcss")||(o.setAttribute("data-loadcss",!0),e.bindMediaToggle(o))}},!e.support()){e.poly();var a=t.setInterval(e.poly,500);t.addEventListener?t.addEventListener("load",function(){e.poly(),t.clearInterval(a)}):t.attachEvent&&t.attachEvent("onload",function(){e.poly(),t.clearInterval(a)})}"undefined"!=typeof exports?exports.loadCSS=loadCSS:t.loadCSS=loadCSS}("undefined"!=typeof global?global:this);</script> <link rel="apple-touch-icon" href="https://files.aptuitivcdn.com/JxEdylMPMz-1515/images/favicon/apple-touch-icon.png"> <link rel="icon" href="https://files.aptuitivcdn.com/JxEdylMPMz-1515/images/favicon/favicon-32x32.png" type="image/png"> <link rel="mask-icon" href="https://files.aptuitivcdn.com/JxEdylMPMz-1515/images/favicon/safari-pinned-tab.svg" color="#ef4035"><meta name="apple-mobile-web-app-title" content="Proteome Sciences"> <meta name="application-name" content="Proteome Sciences"> <meta name="theme-color" content="#ffffff"> <meta name="msapplication-TileColor" content="#ef4035"> <meta name="msapplication-config" content="/browserconfig.xml"> <link rel="manifest" href="/manafest.json"> <script type="application/ld+json">{"@context":"https:\/\/schema.org","@graph":[{"@type":"Organization","url":"https:\/\/www.proteomics.com","@id":"https:\/\/www.proteomics.com\/#organization"},{"@type":"WebSite","url":"https:\/\/www.proteomics.com","@id":"https:\/\/www.proteomics.com\/#website","name":"proteomics.com","potentialAction":{"@type":"SearchAction","target":"https:\/\/www.proteomics.com\/search?q={search_term_string}","query-input":"required name=search_term_string"},"publisher":{"@id":"https:\/\/www.proteomics.com\/#organization"}},{"@type":"WebPage","url":"https:\/\/www.proteomics.com\/services\/discovery-based-proteomics\/tmtcalibrator","@id":"https:\/\/www.proteomics.com\/services\/discovery-based-proteomics\/tmtcalibrator\/#webpage","isPartOf":{"@id":"https:\/\/www.proteomics.com\/#website"},"name":"TMTcalibrator","dateModified":"2022-04-27T10:25:03-04:00"}]}</script> </head> <body> <div class="u-hiddenVisually" aria-hidden="true"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><symbol id="icon-caret-left" viewBox="0 0 8 20"><path d="M7.143 5v10a.719.719 0 01-.714.714.709.709 0 01-.502-.212l-5-5A.71.71 0 01.715 10a.71.71 0 01.212-.502l5-5a.71.71 0 01.502-.212c.391 0 .714.324.714.714z"/></symbol><symbol id="icon-caret-right" viewBox="0 0 6 20"><path d="M6.429 10c0 .19-.078.368-.212.502l-5 5a.71.71 0 01-.502.212A.719.719 0 01.001 15V5c0-.391.324-.714.714-.714.19 0 .368.078.502.212l5 5a.71.71 0 01.212.502z"/></symbol><symbol id="icon-chemistry" viewBox="0 0 38.5 32.4"><path d="M16.554 13.7V2.284h.892a.892.892 0 000-1.784h-10.7a.892.892 0 000 1.784h.892V13.7L.59 29.574a.936.936 0 00.045.847.892.892 0 00.758.4h21.4a.892.892 0 00.758-.4.936.936 0 00.045-.847zm-7.224.535a.847.847 0 00.089-.357V2.284h5.351v11.593a.847.847 0 00.089.357l1.828 4.1H7.502zm-6.555 14.8l3.969-8.918h10.7l3.969 8.918z"/><path d="M31.715 10.4V2.284h.892a.892.892 0 100-1.784h-10.7a.892.892 0 100 1.784h.892V10.4a11.059 11.059 0 00-3.879 3.032.892.892 0 00.134 1.249.847.847 0 001.249-.134 9.186 9.186 0 013.7-2.72.936.936 0 00.58-.847v-8.7h5.351v8.7a.936.936 0 00.58.847 8.785 8.785 0 014.905 4.727H21.147a.892.892 0 100 1.784h14.849a11.5 11.5 0 01.178 1.784 8.963 8.963 0 01-8.914 8.916h-.535c-.535-.045-.8.357-.847.847a.981.981 0 00.892.981h.49a10.747 10.747 0 0010.7-10.746 10.568 10.568 0 00-6.245-9.72z"/></symbol><symbol id="icon-chevron-small-right" viewBox="0 0 20 20"><path d="M11 10L7.859 6.58a.695.695 0 010-.978.68.68 0 01.969 0l3.83 3.908a.697.697 0 010 .979l-3.83 3.908c-.267.272-.701.27-.969 0s-.268-.707 0-.978L11 10z"/></symbol><symbol id="icon-chevron-thin-left" viewBox="0 0 20 20"><path d="M13.891 17.418c.268.272.268.709 0 .979s-.701.271-.969 0l-7.83-7.908a.697.697 0 010-.979l7.83-7.908c.268-.27.701-.27.969 0s.268.709 0 .979L6.75 10l7.141 7.418z"/></symbol><symbol id="icon-chevron-thin-right" viewBox="0 0 20 20"><path d="M13.25 10L6.109 2.58a.697.697 0 010-.979.68.68 0 01.969 0l7.83 7.908a.697.697 0 010 .979l-7.83 7.908c-.268.271-.701.27-.969 0s-.268-.707 0-.979L13.25 10z"/></symbol><symbol id="icon-clipboard" viewBox="0 0 27 33"><path d="M24 3h-6.27a4.482 4.482 0 00-8.46 0H3a3.009 3.009 0 00-3 3v24a3.009 3.009 0 003 3h21a3.009 3.009 0 003-3V6a3.009 3.009 0 00-3-3zM13.5 3A1.5 1.5 0 1112 4.5 1.5 1.5 0 0113.5 3zM24 30H3V6h3v4.5h15V6h3z"/></symbol><symbol id="icon-download" viewBox="0 0 22.55 25.237"><g stroke="currentColor" fill="none" stroke-linecap="round" stroke-width="3" stroke-linejoin="round"><path class="a" d="M11.5 1.5v14.4"/><path class="b" d="M1.5 18.5v5.237h19.55V18.5"/><path class="a" d="M5.55 10.7l6.03 6.03 6.03-6.03"/></g></symbol><symbol id="icon-events" viewBox="0 0 24 24"><path fill="currentColor" d="M19 4h-1V2h-2v2H8V2H6v2H5a2 2 0 00-2 2v14a2 2 0 002 2h14a2 2 0 002-2V6a2 2 0 00-2-2m0 16H5V10h14v10m0-12H5V6h14v2z"/></symbol><symbol id="icon-facebook" viewBox="0 0 24 24"><path fill="currentColor" d="M12 2.04c-5.5 0-10 4.49-10 10.02 0 5 3.66 9.15 8.44 9.9v-7H7.9v-2.9h2.54V9.85c0-2.51 1.49-3.89 3.78-3.89 1.09 0 2.23.19 2.23.19v2.47h-1.26c-1.24 0-1.63.77-1.63 1.56v1.88h2.78l-.45 2.9h-2.33v7a10 10 0 008.44-9.9c0-5.53-4.5-10.02-10-10.02z"/></symbol><symbol id="icon-filter" viewBox="0 0 33.71 29.909"><g fill="none" stroke="currentColor" stroke-width="3" stroke-linecap="round" stroke-linejoin="round"><path class="a" d="M13.406 15.92L1.5 1.5h30.71L19.753 15.884v6.179l-6.347 6.346z"/><path class="b" d="M5.981 6.46h16.34"/></g></symbol><symbol id="icon-instagram" viewBox="0 0 17 20"><path d="M11.429 10c0-1.574-1.283-2.857-2.857-2.857S5.715 8.426 5.715 10s1.283 2.857 2.857 2.857 2.857-1.283 2.857-2.857zm1.54 0c0 2.433-1.964 4.397-4.397 4.397S4.175 12.433 4.175 10s1.964-4.397 4.397-4.397S12.969 7.567 12.969 10zm1.205-4.576c0 .569-.458 1.027-1.027 1.027s-1.027-.458-1.027-1.027.458-1.027 1.027-1.027 1.027.458 1.027 1.027zM8.571 2.969c-1.25 0-3.929-.1-5.056.346-.391.156-.681.346-.982.647s-.491.592-.647.982C1.44 6.071 1.54 8.75 1.54 10s-.1 3.929.346 5.056c.156.391.346.681.647.982s.592.491.982.647c1.127.446 3.806.346 5.056.346s3.929.1 5.056-.346c.391-.156.681-.346.982-.647s.491-.592.647-.982c.446-1.127.346-3.806.346-5.056s.1-3.929-.346-5.056c-.156-.391-.346-.681-.647-.982s-.592-.491-.982-.647c-1.127-.446-3.806-.346-5.056-.346zM17.143 10c0 1.183.011 2.355-.056 3.538-.067 1.373-.379 2.589-1.384 3.594s-2.221 1.317-3.594 1.384c-1.183.067-2.355.056-3.538.056s-2.355.011-3.538-.056c-1.373-.067-2.589-.379-3.594-1.384S.122 14.911.055 13.538C-.012 12.355-.001 11.183-.001 10s-.011-2.355.056-3.538c.067-1.373.379-2.589 1.384-3.594S3.66 1.551 5.033 1.484c1.183-.067 2.355-.056 3.538-.056s2.355-.011 3.538.056c1.373.067 2.589.379 3.594 1.384s1.317 2.221 1.384 3.594c.067 1.183.056 2.355.056 3.538z"/></symbol><symbol id="icon-linkedin" viewBox="0 0 24 24"><path fill="currentColor" d="M19 3a2 2 0 012 2v14a2 2 0 01-2 2H5a2 2 0 01-2-2V5a2 2 0 012-2h14m-.5 15.5v-5.3a3.26 3.26 0 00-3.26-3.26c-.85 0-1.84.52-2.32 1.3v-1.11h-2.79v8.37h2.79v-4.93c0-.77.62-1.4 1.39-1.4a1.4 1.4 0 011.4 1.4v4.93h2.79M6.88 8.56a1.68 1.68 0 001.68-1.68c0-.93-.75-1.69-1.68-1.69a1.69 1.69 0 00-1.69 1.69c0 .93.76 1.68 1.69 1.68m1.39 9.94v-8.37H5.5v8.37h2.77z"/></symbol><symbol id="icon-news" viewBox="0 0 24 24"><path fill="currentColor" d="M4 7v12h15v2H4c-2 0-2-2-2-2V7h2m17-2v10H8V5h13m.3-2H7.7C6.76 3 6 3.7 6 4.55v10.9c0 .86.76 1.55 1.7 1.55h13.6c.94 0 1.7-.69 1.7-1.55V4.55C23 3.7 22.24 3 21.3 3M9 6h3v5H9V6m11 8H9v-2h11v2m0-6h-6V6h6v2m0 3h-6V9h6v2z"/></symbol><symbol id="icon-open-book" viewBox="0 0 24 24"><path fill="currentColor" d="M17.5 14.33c.79 0 1.63.08 2.5.24v1.5c-.62-.16-1.46-.24-2.5-.24-1.9 0-3.39.33-4.5.99v-1.69c1.17-.53 2.67-.8 4.5-.8M13 12.46c1.29-.53 2.79-.79 4.5-.79.79 0 1.63.07 2.5.23v1.5c-.62-.16-1.46-.24-2.5-.24-1.9 0-3.39.34-4.5.99m4.5-3.65c-1.9 0-3.39.32-4.5 1V9.84c1.23-.56 2.73-.84 4.5-.84.79 0 1.63.08 2.5.23v1.55c-.74-.19-1.59-.28-2.5-.28m3.5 8V7c-1.04-.33-2.21-.5-3.5-.5-2.05 0-3.88.5-5.5 1.5v11.5c1.62-1 3.45-1.5 5.5-1.5 1.19 0 2.36.16 3.5.5m-3.5-14c2.35 0 4.19.5 5.5 1.5v14.56c0 .12-.05.24-.16.35-.11.09-.23.17-.34.17-.11 0-.19-.02-.25-.05-1.28-.69-2.87-1.03-4.75-1.03-2.05 0-3.88.5-5.5 1.5-1.34-1-3.17-1.5-5.5-1.5-1.66 0-3.25.36-4.75 1.07-.03.01-.07.01-.12.03-.04.01-.08.02-.13.02-.11 0-.23-.04-.34-.12a.475.475 0 01-.16-.35V6c1.34-1 3.18-1.5 5.5-1.5 2.33 0 4.16.5 5.5 1.5 1.34-1 3.17-1.5 5.5-1.5z"/></symbol><symbol id="icon-pinterest" viewBox="0 0 14 20"><path d="M0 6.663C0 2.545 3.772 0 7.589 0c3.504 0 6.696 2.411 6.696 6.105 0 3.471-1.775 7.321-5.725 7.321-.938 0-2.121-.469-2.578-1.339-.848 3.359-.781 3.862-2.656 6.429l-.156.056-.1-.112c-.067-.703-.167-1.395-.167-2.098 0-2.277 1.049-5.569 1.563-7.779-.279-.569-.357-1.261-.357-1.886 0-1.127.781-2.556 2.054-2.556.938 0 1.44.714 1.44 1.596 0 1.451-.982 2.813-.982 4.219 0 .96.792 1.629 1.719 1.629 2.567 0 3.359-3.705 3.359-5.681 0-2.645-1.875-4.085-4.408-4.085-2.946 0-5.223 2.121-5.223 5.112 0 1.44.882 2.176.882 2.522 0 .29-.212 1.317-.58 1.317-.056 0-.134-.022-.19-.033C.584 10.257.004 8.125.004 6.663z"/></symbol><symbol id="icon-search" viewBox="0 0 19 20"><path d="M12.857 9.286c0-2.757-2.243-5-5-5s-5 2.243-5 5 2.243 5 5 5 5-2.243 5-5zm5.714 9.285c0 .781-.647 1.429-1.429 1.429-.379 0-.748-.156-1.004-.424l-3.828-3.817a7.839 7.839 0 01-4.453 1.384C3.515 17.143 0 13.627 0 9.286s3.516-7.857 7.857-7.857a7.855 7.855 0 016.473 12.31l3.828 3.828c.257.257.413.625.413 1.004z"/></symbol><symbol id="icon-shopping-cart" viewBox="0 0 19 20"><path d="M7.143 17.143c0 .781-.647 1.429-1.429 1.429s-1.429-.647-1.429-1.429.647-1.429 1.429-1.429 1.429.647 1.429 1.429zm10 0c0 .781-.647 1.429-1.429 1.429s-1.429-.647-1.429-1.429.647-1.429 1.429-1.429 1.429.647 1.429 1.429zM18.571 5v5.714a.73.73 0 01-.636.714L6.283 12.79c.056.257.145.513.145.781 0 .257-.156.491-.268.714h10.268c.391 0 .714.324.714.714s-.324.714-.714.714H4.999a.719.719 0 01-.714-.714c0-.346.502-1.183.681-1.529L2.991 4.285H.714C.323 4.285 0 3.961 0 3.571s.324-.714.714-.714h2.857c.748 0 .77.893.882 1.429h13.404c.391 0 .714.324.714.714z"/></symbol><symbol id="icon-twitter" viewBox="0 0 19 20"><path d="M18.08 4.554a7.768 7.768 0 01-1.808 1.864c.011.156.011.313.011.469 0 4.766-3.627 10.257-10.257 10.257-2.042 0-3.94-.592-5.536-1.618.29.033.569.045.871.045a7.217 7.217 0 004.475-1.54 3.61 3.61 0 01-3.371-2.5 4.6 4.6 0 00.681.056c.324 0 .647-.045.949-.123a3.606 3.606 0 01-2.891-3.538v-.045c.48.268 1.038.435 1.629.458a3.601 3.601 0 01-1.607-3.002c0-.67.179-1.283.491-1.819A10.248 10.248 0 009.15 7.29a4.057 4.057 0 01-.089-.826 3.603 3.603 0 013.605-3.605c1.038 0 1.975.435 2.634 1.138a7.089 7.089 0 002.288-.871 3.598 3.598 0 01-1.585 1.987 7.226 7.226 0 002.076-.558z"/></symbol><symbol id="icon-updates" viewBox="0 0 36 36"><path d="M31.5 15.18H21.33l4.11-4.23a10.558 10.558 0 00-14.82-.15 10.313 10.313 0 000 14.685 10.529 10.529 0 0014.82 0 9.77 9.77 0 003.06-7.335h3a14.011 14.011 0 01-3.96 9.435 13.581 13.581 0 01-19.08 0 13.26 13.26 0 01-.03-18.87 13.481 13.481 0 0118.975 0L31.5 4.5zM18.75 12v6.375l5.25 3.12-1.08 1.815-6.42-3.81V12z"/></symbol><symbol id="icon-youtube" viewBox="0 0 17 20"><path d="M10.837 13.884v2.355c0 .502-.145.748-.435.748-.167 0-.335-.078-.502-.246v-3.359c.167-.167.335-.246.502-.246.29 0 .435.257.435.748zm3.772.011v.513h-1.004v-.513c0-.502.167-.759.502-.759s.502.257.502.759zM3.828 11.462h1.194v-1.049H1.54v1.049h1.172v6.35h1.116v-6.35zm3.214 6.351h.993V12.3h-.993v4.219c-.223.313-.435.469-.636.469-.134 0-.212-.078-.234-.234-.011-.033-.011-.156-.011-.391V12.3h-.993v4.364c0 .391.033.647.089.815.089.279.324.413.647.413.357 0 .737-.223 1.138-.681v.603zm4.788-1.652v-2.199c0-.513-.022-.882-.1-1.105-.123-.413-.402-.625-.792-.625-.368 0-.714.201-1.038.603v-2.422h-.993v7.4H9.9v-.536c.335.413.681.614 1.038.614.391 0 .67-.212.792-.614.078-.234.1-.603.1-1.116zm3.773-.112v-.145h-1.016c0 .402-.011.625-.022.681-.056.268-.201.402-.446.402-.346 0-.513-.257-.513-.77v-.971h1.998v-1.15c0-.592-.1-1.016-.301-1.295-.29-.379-.681-.569-1.183-.569-.513 0-.904.19-1.194.569-.212.279-.313.703-.313 1.295v1.931c0 .592.112 1.027.324 1.295.29.379.681.569 1.205.569s.938-.201 1.205-.592c.123-.179.212-.379.234-.603.022-.1.022-.324.022-.647zM8.817 5.859V3.515c0-.513-.145-.77-.48-.77-.324 0-.48.257-.48.77v2.344c0 .513.156.781.48.781.335 0 .48-.268.48-.781zm8.025 8.382c0 1.283-.011 2.656-.29 3.906-.212.882-.926 1.529-1.786 1.618-2.054.234-4.129.234-6.194.234s-4.141 0-6.194-.234c-.859-.089-1.585-.737-1.786-1.618-.29-1.25-.29-2.623-.29-3.906 0-1.295.011-2.656.29-3.906.212-.882.926-1.529 1.797-1.629 2.042-.223 4.118-.223 6.183-.223s4.141 0 6.194.223a2.1 2.1 0 011.786 1.629c.29 1.25.29 2.612.29 3.906zM5.703 0h1.138l-1.35 4.453v3.025H4.375V4.453c-.1-.547-.324-1.328-.681-2.366C3.448 1.395 3.203.692 2.969 0h1.183l.792 2.935zm4.13 3.717V5.67c0 .592-.1 1.038-.313 1.317-.279.379-.67.569-1.183.569-.502 0-.893-.19-1.172-.569-.212-.29-.313-.725-.313-1.317V3.717c0-.592.1-1.027.313-1.306.279-.379.67-.569 1.172-.569.513 0 .904.19 1.183.569.212.279.313.714.313 1.306zm3.738-1.809v5.569h-1.016v-.614c-.402.469-.781.692-1.15.692-.324 0-.558-.134-.658-.413-.056-.167-.089-.435-.089-.837V1.908h1.016v4.096c0 .234 0 .368.011.391.022.156.1.246.234.246.201 0 .413-.156.636-.48V1.909h1.016z"/></symbol></svg></div> <header class="Header js-header" id="top"> <div class="Container"> <div class="Grid Grid--withGutter Grid--alignMiddle"> <div class="Grid-cell u-size1of5 u-md-sizeFull u-md-margBottom2 u-md-textCenter"> <a href="/" title="Back to home page"> <div class="Header-logo Header-hideOnSticky"> <svg xmlns="http://www.w3.org/2000/svg" width="173.2" height="101.452" viewBox="0 0 173.2 101.452"><defs><style>.a{fill:#fff;}</style></defs><path class="a expand" d="M 130.281,67.7 V 95.26 H 41.24 V 67.7 h -6.1 v 33.66 H 136.385 V 67.7 Z"/><path class="a expand" d="M34.58,0V33.449h6.108V6.1h89.041V33.449h6.1V0Z" transform=""/><path class="a red" d="M146.6,86.434h4.453V90.5H146.6v10.964c0,2.054.516,2.569,2.569,2.569a9.318,9.318,0,0,0,1.883-.175v4.756a23.417,23.417,0,0,1-3.555.212c-3.742,0-6.981-.856-6.981-5.268V81H146.6v5.447" transform="translate(-82.096 -47.323)"/><path class="a red" d="M12.473,92.607a7.369,7.369,0,0,0-6.594,3.426h-.1V93.21H0v28.75H6.079v-9.355h.087a7.846,7.846,0,0,0,6.652,3.326c6.465,0,9.721-5.526,9.721-11.434,0-6.295-3.127-11.908-10.062-11.908m-1.285,18.759c-3.941,0-5.309-3.426-5.309-7.022s1.285-7.155,5.264-7.155c3.9,0,5.314,3.642,5.314,7.155-.017,3.613-1.3,7.039-5.284,7.039Z" transform="translate(0 -54.094)"/><path class="a red" d="M63.091,102.521v12.847H57V93.229h5.738v3h.083c1.584-2.27,2.761-3.289,5.592-3.6v4.648a4.861,4.861,0,0,0-5.334,5.243" transform="translate(-33.301 -54.117)"/><path class="a red" d="M178.373,92.63c-6.652,0-11.163,4.989-11.163,11.546,0,6.765,4.27,11.5,11.163,11.5,4.943,0,8.54-2.2,10.232-7.355h-5.268c-.378,1.351-2.324,2.831-4.736,2.831-3.326,0-5.2-1.73-5.368-5.538H189c.416-6.723-3.172-12.98-10.61-12.98m-5.18,9.176a4.731,4.731,0,0,1,4.989-4.652c2.91,0,4.228,1.609,4.777,4.652Z" transform="translate(-97.689 -54.117)"/><path class="a red" d="M375.313,92.63c-6.652,0-11.163,4.989-11.163,11.546,0,6.765,4.27,11.5,11.163,11.5,4.948,0,8.54-2.2,10.232-7.355h-5.3c-.383,1.351-2.324,2.831-4.736,2.831-3.326,0-5.2-1.73-5.372-5.538h15.774c.416-6.723-3.172-12.98-10.615-12.98m-5.16,9.176a4.734,4.734,0,0,1,4.989-4.652c2.91,0,4.228,1.609,4.777,4.652Z" transform="translate(-212.748 -54.117)"/><path class="a red" d="M94.538,92.63c-6.848,0-11.288,4.573-11.288,11.546s4.44,11.5,11.288,11.5,11.334-4.573,11.334-11.5S101.44,92.63,94.538,92.63m0,18.518c-4.058,0-5.284-3.509-5.284-6.972s1.247-7.022,5.284-7.022,5.33,3.513,5.33,7.022S98.642,111.148,94.538,111.148Z" transform="translate(-48.637 -54.117)"/><path class="a red" d="M233.678,92.63c-6.848,0-11.288,4.573-11.288,11.546s4.44,11.5,11.288,11.5,11.334-4.573,11.334-11.5-4.44-11.546-11.334-11.546m0,18.518c-4.058,0-5.284-3.509-5.284-6.972s1.227-7.022,5.284-7.022,5.33,3.513,5.33,7.022S237.782,111.148,233.678,111.148Z" transform="translate(-129.927 -54.117)"/><path class="a red" d="M313.823,100.554v14.814H307.74V102.824c0-2.956-.258-5.4-3.742-5.4s-4.041,2.848-4.041,5.617v12.332h-6.091V102.953c0-2.569.17-5.526-3.684-5.526-1.2,0-4.108.769-4.108,5.1v12.843H280V93.232h5.738v3h.083a8,8,0,0,1,6.765-3.6c2.786,0,5.268,1.031,6.465,3.684a8.053,8.053,0,0,1,6.681-3.684c4.711,0,8.1,2.187,8.1,7.925" transform="translate(-163.585 -54.117)"/><path class="a" d="M112.872,173.521c-2.445,0-4.831-.919-4.952-3.709h2.748c0,1.247,1.081,1.775,2.224,1.775.832,0,1.892-.328,1.892-1.347,0-.873-1.2-1.181-3.28-1.63-1.663-.366-3.326-.956-3.326-2.811,0-2.69,2.32-3.38,4.573-3.38s4.42.773,4.644,3.359h-2.748c-.083-1.118-.94-1.426-1.975-1.426-.653,0-1.613.125-1.613.977,0,1.039,1.63,1.185,3.28,1.572s3.326,1,3.326,2.952c0,2.769-2.4,3.667-4.806,3.667" transform="translate(-63.05 -94.891)"/><path class="a" d="M139.064,173.509a5.112,5.112,0,0,1-5.314-5.4c0-3.28,1.954-5.7,5.376-5.7,2.495,0,4.769,1.3,4.952,3.991h-2.831a1.975,1.975,0,0,0-2.141-1.813c-1.854,0-2.465,1.875-2.465,3.422s.59,3.326,2.4,3.326a2.227,2.227,0,0,0,2.3-2.141h2.79a4.7,4.7,0,0,1-5.072,4.32" transform="translate(-78.141 -94.879)"/><rect class="a" width="2.894" height="10.531" transform="translate(67.251 67.812)"/><path class="a" d="M174.644,168.673c.079,1.834.977,2.669,2.586,2.669a2.4,2.4,0,0,0,2.278-1.368h2.549a4.744,4.744,0,0,1-4.931,3.547c-3.326,0-5.376-2.283-5.376-5.542a5.28,5.28,0,0,1,5.376-5.559c3.588,0,5.318,3.014,5.114,6.237h-7.6m2.4-4.075a2.278,2.278,0,0,0-2.4,2.241h4.694c-.254-1.451-.886-2.224-2.291-2.224Z" transform="translate(-100.342 -94.891)"/><path class="a" d="M206.742,173.234v-5.945c0-1.734-.507-2.586-1.85-2.586-1.572,0-2.241.873-2.241,3.014v5.517h-2.91V162.7h2.748v1.468h.062a3.742,3.742,0,0,1,3.218-1.75c3.077,0,3.85,1.73,3.85,4.336v6.478h-2.91" transform="translate(-116.694 -94.89)"/><path class="a" d="M231.9,173.509a5.115,5.115,0,0,1-5.318-5.4c0-3.28,1.958-5.7,5.38-5.7,2.495,0,4.765,1.3,4.948,3.991h-2.831a1.971,1.971,0,0,0-2.137-1.813c-1.854,0-2.465,1.875-2.465,3.422s.59,3.326,2.4,3.326a2.227,2.227,0,0,0,2.3-2.141h2.79a4.7,4.7,0,0,1-5.072,4.32" transform="translate(-132.375 -94.879)"/><path class="a" d="M256.94,168.673c.083,1.834.977,2.669,2.59,2.669a2.4,2.4,0,0,0,2.278-1.368h2.549a4.744,4.744,0,0,1-4.931,3.547c-3.326,0-5.376-2.283-5.376-5.542a5.28,5.28,0,0,1,5.376-5.559c3.584,0,5.318,3.014,5.114,6.237h-7.6m2.407-4.075a2.283,2.283,0,0,0-2.407,2.241h4.7c-.262-1.451-.894-2.224-2.3-2.224Z" transform="translate(-148.424 -94.891)"/><path class="a" d="M286.222,173.521c-2.445,0-4.827-.919-4.952-3.709h2.752c.021,1.247,1.077,1.775,2.22,1.775.832,0,1.892-.328,1.892-1.347,0-.873-1.2-1.181-3.276-1.63-1.663-.366-3.326-.956-3.326-2.811,0-2.69,2.324-3.38,4.573-3.38s4.424.773,4.648,3.359H288c-.079-1.118-.935-1.426-1.975-1.426-.653,0-1.609.125-1.609.977,0,1.039,1.63,1.185,3.28,1.572s3.326,1,3.326,2.952c0,2.769-2.4,3.667-4.806,3.667" transform="translate(-164.327 -94.891)"/></svg> </div> <div class="Header-logo Header-showOnSticky"> <img class="Header-logo" src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/images/logo-color.1654711795.svg" alt="Proteome Sciences"> </div> </a> </div> <div class="Grid-cell u-size4of5 u-hidden u-textRight u-lg-show u-md-sizeFull u-md-textCenter u-xl-show"> <a class="Header-search u-padRight1" href="/search"><svg class="Icon" role="img" alt="Search" aria-labelledby="iconTitle-bbAyWj" focusable="false"><title id="">Search</title><use xlink:href="#icon-search" /></svg></a> <button class="SmScBtn js-ssNavBtn"><span class="SmScBtn-icon"></span>Menu</button> <a class="Button Button--secondary" href="/contact">contact us</a> </div> <div class="Grid-cell u-size4of5 u-textRight u-xl-sizeFull u-xl-textCenter"> <div class="NavBar" role="navigation" aria-label="main navigation"> <ul class="MainNav js-mainNav" data-access-nav role="menubar"> <li class="MainNav-item is-first" role="presentation"> <a href="/" class="is-first MainNav-link js-navLink" role="menuitem">Home</a> </li> <li class="MainNav-item Dropdown js-dropdownParent" role="presentation"> <a href="/about" class="MainNav-link js-navLink is-dropdown js-dropdown" aria-haspopup="true" id="JbMySjnm" role="menuitem">About Us</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="JbMySjnm" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item is-first" role="presentation"> <a href="/about" class="is-first Dropdown-link js-navLink" role="menuitem">Company</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/contact" class="Dropdown-link js-navLink" role="menuitem">Contact</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/about/leadership" class="Dropdown-link js-navLink" role="menuitem">Leadership</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/about/facilities" class="Dropdown-link js-navLink" role="menuitem">Facilities</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/about/careers" class="Dropdown-link js-navLink" role="menuitem">Careers</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/about/tandem-mass-tags" class="Dropdown-link js-navLink" role="menuitem">Tandem Mass Tags (TMT)</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/about/trademarks-copyrights" class="is-last Dropdown-link js-navLink" role="menuitem">Trademarks & Copyrights</a> </li> </ul> </div> </li> <li class="MainNav-item is-currentParent Dropdown js-dropdownParent" role="presentation"> <a href="/services/targeted-proteomics" class="is-currentParent MainNav-link js-navLink is-dropdown js-dropdown" aria-haspopup="true" id="7namRMe4" role="menuitem">Services</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="7namRMe4" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item Dropdown-item--smallScreen js-skip" role="presentation"><a href="/services/targeted-proteomics" class="Dropdown-link" aria-haspopup="true" role="menuitem">Services</a></li> <li class="Dropdown-item is-first" role="presentation"> <a href="/services" class="is-first Dropdown-link js-navLink" role="menuitem">About our Services</a> </li> <li class="Dropdown-item is-currentParent is-firstParent js-dropdownParent" role="presentation"> <a href="/services/discovery-based-proteomics" class="is-currentParent is-firstParent Dropdown-link js-navLink Dropdown-link--parent js-dropdown" aria-haspopup="true" id="rbpxKHFp" role="menuitem">Discovery Based Proteomics</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="rbpxKHFp" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item is-first" role="presentation"> <a href="/services/discovery-based-proteomics" class="is-first Dropdown-link js-navLink" role="menuitem">Advantages</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/label-free-versus-tandem-mass-tags" class="Dropdown-link js-navLink" role="menuitem">Label Free Versus Tandem Mass Tags</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/discovery-based-proteomics/tmt-based-experiments" class="Dropdown-link js-navLink" role="menuitem">TMT based LC-MS2 and LC-MS3</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/discovery-based-proteomics/ptms-with-sysquant" class="Dropdown-link js-navLink" role="menuitem">Post Translational Modifications</a> </li> <li class="Dropdown-item is-current" role="presentation"> <a href="/services/discovery-based-proteomics/tmtcalibrator" class="is-current Dropdown-link js-navLink" role="menuitem">TMTcalibrator</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/discovery-based-proteomics/super-depletion" class="Dropdown-link js-navLink" role="menuitem">Super Depletion</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/speciality-proteomics/computational-proteomics" class="Dropdown-link js-navLink" role="menuitem">Mass Spectrometry in the AI/ML area</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/services/speciality-proteomics/protein-degrader" class="is-last Dropdown-link js-navLink" role="menuitem">Protein Degrader/PROTAC assays</a> </li> </ul> </div> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/single-cell-proteomcs" class="Dropdown-link js-navLink" role="menuitem">Single Cell Proteomics</a> </li> <li class="Dropdown-item js-dropdownParent" role="presentation"> <a href="/services/speciality-proteomics" class="Dropdown-link js-navLink Dropdown-link--parent js-dropdown" aria-haspopup="true" id="v4Tqmg9N" role="menuitem">Speciality Proteomics</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="v4Tqmg9N" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item is-first" role="presentation"> <a href="/services/speciality-proteomics" class="is-first Dropdown-link js-navLink" role="menuitem">Overview</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/speciality-proteomics/ip-pull-downs" class="Dropdown-link js-navLink" role="menuitem">IP Pull Down Sample Pre-Treatment</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/speciality-proteomics/off-gel-protein-sequencing" class="Dropdown-link js-navLink" role="menuitem">Off-Gel protein sequencing</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/speciality-proteomics/peptidomics" class="Dropdown-link js-navLink" role="menuitem">Peptidomics and MHC I/II Immunopeptidomics</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/speciality-proteomics/protein-degrader" class="Dropdown-link js-navLink" role="menuitem">Protein Degrader/PROTAC Assays</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/services/speciality-proteomics/computational-proteomics" class="is-last Dropdown-link js-navLink" role="menuitem">Computational Proteomics Driven by Machine Learning and Artificial Intelligence</a> </li> </ul> </div> </li> <li class="Dropdown-item js-dropdownParent" role="presentation"> <a href="/services/" class="Dropdown-link js-navLink Dropdown-link--parent js-dropdown" aria-haspopup="true" id="tXK3fF6D" role="menuitem">Targeted Assays</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="tXK3fF6D" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item Dropdown-item--smallScreen js-skip" role="presentation"><a href="/services/" class="Dropdown-link" aria-haspopup="true" role="menuitem">Targeted Assays</a></li> <li class="Dropdown-item is-first" role="presentation"> <a href="/services/targeted-proteomics" class="is-first Dropdown-link js-navLink" role="menuitem">Overview</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/targeted-proteomics/developed-targeted-assay" class="Dropdown-link js-navLink" role="menuitem">Developing a targeted assay</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/multiplexing-of-tryptophan-and-its-metabolites-pre-launch" class="is-last Dropdown-link js-navLink" role="menuitem">Tryptophan and its Metabolites</a> </li> </ul> </div> </li> <li class="Dropdown-item js-dropdownParent" role="presentation"> <a href="/services/quality-management" class="Dropdown-link js-navLink Dropdown-link--parent js-dropdown" aria-haspopup="true" id="AXz6vYdy" role="menuitem">Quality Management</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="AXz6vYdy" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item is-first" role="presentation"> <a href="/services/quality-management" class="is-first Dropdown-link js-navLink" role="menuitem">Summary</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/quality-management/iso-ceritifcation" class="Dropdown-link js-navLink" role="menuitem">ISO9001:2015 Certification</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/services/quality-management/gclp-compliance" class="is-last Dropdown-link js-navLink" role="menuitem">GCLP Compliance</a> </li> </ul> </div> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/bioinformatics-package" class="Dropdown-link js-navLink" role="menuitem">Bioinformatics</a> </li> <li class="Dropdown-item is-last js-dropdownParent" role="presentation"> <a href="/services/" class="is-last Dropdown-link js-navLink Dropdown-link--parent js-dropdown" aria-haspopup="true" id="tQc7R7fJ" role="menuitem">Working with us</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="tQc7R7fJ" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item Dropdown-item--smallScreen js-skip" role="presentation"><a href="/services/" class="Dropdown-link" aria-haspopup="true" role="menuitem">Working with us</a></li> <li class="Dropdown-item is-first" role="presentation"> <a href="/services/working-with-us" class="is-first Dropdown-link js-navLink" role="menuitem">Summary of our Proteomic Servies</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/working-with-us/types-and-quantities-of-samples-required-for-analysis" class="Dropdown-link js-navLink" role="menuitem">Types and Quantities of Samples Required for Analysis</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/services/how-to-work-with-us" class="Dropdown-link js-navLink" role="menuitem">How to discuss and order a project with us</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/services/how-to-work-with-us/working-with-us" class="is-last Dropdown-link js-navLink" role="menuitem">Sample analysis and reporting</a> </li> </ul> </div> </li> </ul> </div> </li> <li class="MainNav-item Dropdown js-dropdownParent" role="presentation"> <a href="/news" class="MainNav-link js-navLink is-dropdown js-dropdown" aria-haspopup="true" id="XNwmHsZY" role="menuitem">News & Events</a> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="XNwmHsZY" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item is-first" role="presentation"> <a href="/news" class="is-first Dropdown-link js-navLink" role="menuitem">News</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/events" class="is-last Dropdown-link js-navLink" role="menuitem">Events</a> </li> </ul> </div> </li> <li class="MainNav-item Dropdown js-dropdownParent" role="presentation"> <span class="MainNav-link js-navLink is-dropdown js-dropdown">Resources</span> <div class="Dropdown-wrapper js-dropdownWrapper"> <ul class="Dropdown-menu js-dropdownMenu" role="menu" aria-labelledby="BSMSsAJQ" aria-expanded="false" aria-hidden="true"> <li class="Dropdown-item Dropdown-item--smallScreen js-skip" role="presentation"><a href="#" class="Dropdown-link" aria-haspopup="true" role="menuitem">Resources</a></li> <li class="Dropdown-item is-first" role="presentation"> <a href="/resources/case-studies" class="is-first Dropdown-link js-navLink" role="menuitem">Case Studies</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/resources/publications" class="Dropdown-link js-navLink" role="menuitem">Publications</a> </li> <li class="Dropdown-item" role="presentation"> <a href="/resources/posters" class="Dropdown-link js-navLink" role="menuitem">Posters</a> </li> <li class="Dropdown-item is-last" role="presentation"> <a href="/resources/faqs" class="is-last Dropdown-link js-navLink" role="menuitem">FAQs</a> </li> </ul> </div> </li> <li class="MainNav-item is-last" role="presentation"> <a href="/investors" class="is-last MainNav-link js-navLink" role="menuitem">Investors</a> </li> </ul> <a class="Header-search u-padLeft1 u-xl-hide" href="/search"><svg class="Icon" role="img" alt="Search" aria-labelledby="iconTitle-bbAyWj" focusable="false"><title id="">Search</title><use xlink:href="#icon-search" /></svg></a> <span class="Header-hideOnSticky u-padLeft2 u-lg-padLeft0"> <a class="Button Button--secondary u-xl-hide" href="/contact">contact us</a> </span> <span class="Header-showOnSticky u-padLeft2"> <a class="Button u-lg-hide" href="/contact">contact us</a> </span> </div> </div> </div> </div> </header> <div class="TextHeader"> <div class="Container"> <div class="TextHeader-content"> <h1 class="TextHeader-title u-redlineLeft">Discovery Proteomics: TMTcalibrator</h1> </div> </div> </div> <main> <div class="Container"> <div class="Content u-cf"> <h2>Advantages</h2> <ul> <li>The presence of tissue trigger channels in the TMT based experiment significantly increases the disease specific protein identifications in the other fluid channels.</li> <li>Ability to enrich for specific protein subsets, such as secreted proteins, phosphopeptides and other post translational modifications and membrane proteins.</li> <li>Due to the unbiased nature of the analysis, we report all proteins and associated PTM’s that are detectable, unlike antibody or aptamer/affimer based technologies where the data is biased to the make-up of the selected analysis panel.</li> <li>By using an upfront high abundance protein depletion stage, Super Depletion, we can quantify many more lower abundant proteins in plasma and serum.</li> <li>Multiplexing multiple samples in a single TMT based mass spectrometry run significantly increases the sensitivity of protein detection.</li> <li>Compatibility with a wide variety of sample matrices, including plasma, serum, cell lysates, CSF, exosomes etc.</li> <li>Available for human, rodent, monkey, pig and other higher animal species.</li> <li>With TMT, multiple samples can be analysed on one mass spectrometric run. Using a common reference channel per plex we can quantify samples from 14 to 100+ sample cohorts in multiple mass spectrometric runs.</li> <li>Analysis package includes full computational proteomic analysis and bioinformatics covering extensive data interpretation, pathway analysis and biological relevance.</li> </ul> <table class="Table--themed" style="border-collapse: collapse; width: 99.9211%;" border="1"> <thead> <tr> <th style="width: 23.4467%;" scope="col">Application</th> <th style="width: 30.2793%;" scope="col">Service</th> <th style="width: 44.6954%;" scope="col">Description</th> </tr> </thead> <tbody> <tr> <td style="width: 23.4467%;">Functional Biomarker Discovery</td> <td style="width: 30.2793%;"> <div>TMTcalibrator 11-plex</div> <div>TMTcalibrator 18-plex</div> </td> <td style="width: 44.6954%;"> <ul> <li>Incorporates a specific tissue/cell trigger into the TMT assay to enhance detection of tissue derived proteins in fluid matrix</li> <li>Relative protein quantification</li> <li>Combine with abundant protein depletion in plasma</li> <li>Generally doubles detection rate of proteins in fluid matrix</li> </ul> </td> </tr> <tr> <td style="width: 23.4467%;">Targeted Biomarker Assays</td> <td style="width: 30.2793%;"> <div>TMTcalibrator 11-plex</div> <div>TMTcalibrator 18-plex</div> </td> <td style="width: 44.6954%;"> <ul> <li>Calibrator channels used to provide relative or absolute concentration standards</li> <li>Multi-point calibration curve within the same spectrum as peptide sequence</li> <li>Combine with LC-MS3 for most accurate quantification</li> <li>Further sensitivity enhancements with target immunoprecipitation, off-line fractionation and high-spike trigger channels</li> </ul> </td> </tr> </tbody> </table> <h2>Magnifying the search for circulating Biomarkers</h2> <p>Looking for functional biomarkers (those related to relevant biology) in body fluids faces several challenges. Fluid-derived proteins are generally present at low concentrations, whilst higher abundance proteins restrict the amount we can load into mass spectrometers. </p> <p>To overcome those limitations we invented TMTcalibrator where we use the isobaric labelling with TMT or TMTpro to mix sources enriched with biomarkers (e.g. diseased tissue lysates) and peripheral fluids (Figure 1). By adding the content of tissue and fluid channels in a single analytical sample we substantially increase visibility of highly relevant peptides and proteins and improve the rate of discovery of valuable biomarkers.</p> <p>TMTcalibrator can be combined with other workflows such as phosphopeptide enrichment, peptidomics and triggering with synthetic peptides depending on your specific needs.</p> <div class="Grid Grid--withGutter u-margTop3 u-margBottom5"> <div class="Grid-cell u-size2of5 u-md-sizeFull u-gutters4 u-textCenter"> <div class="PopImage"><img src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/images/tmtpro-super-deletion.png" height="375" width="500" class="PopImage-image u-sizeFull u-lg-sizeAuto u-sm-sizeFull" alt=""></div> </div> <div class="Grid-cell u-size3of5 u-md-sizeFull u-md-gutters4"> <p>TMTcalibrator tissue-enhanced biomarker discovery. Inclusion of an excess of tissue-derived proteins (trigger/calibrant) in the TMTcalibrator workflow boosts the signal intensity of any homologous proteins in the peripheral fluid channels relative to more abundant peripheral fluid proteins not present in tissue. This selective amplification allows more tissue-derived proteins to be identified and quantified using the data-dependent acquisition method for unbiased biomarker discovery. Fluid concentrations are calculated relative to the tissue trigger and comparison of expression between clinical groups can then be determined using standard bioinformatics methods</p> </div> </div> <h2>Sample requirements for TMTcalibrator analysis (per 18-plex experiment)</h2> <table class="Table--themed" style="border-collapse: collapse; width: 99.9211%;" border="1"> <thead> <tr> <th style="width: 24.4771%;" scope="col">Application</th> <th style="width: 24.4771%;" scope="col"> <p>Plasma/Serum</p> <p>(per sample)</p> </th> <th style="width: 24.4771%;" scope="col"> <p>CSF</p> <p>(per sample)</p> </th> <th style="width: 24.4771%;" scope="col"> <p>Tissue Trigger</p> <p>(total protein)</p> </th> </tr> </thead> <tbody> <tr> <td style="width: 24.4771%;">Standard <a href="/services/tmt-ms2-workflow">LC-MS2 </a></td> <td style="width: 24.4771%;">100ul</td> <td style="width: 24.4771%;">250ul</td> <td style="width: 24.4771%;">1.3 mg</td> </tr> <tr> <td style="width: 24.4771%;"><a href="/services/tmt-ms2-workflow">LC-MS2</a> with upfront <a href="/services/discovery-based-proteomics/super-depletion">Super Depletion</a></td> <td style="width: 24.4771%;">250ul</td> <td style="width: 24.4771%;">Not applicable</td> <td style="width: 24.4771%;">1.3 mg</td> </tr> <tr> <td style="width: 24.4771%;"> <p><a href="/services/sysquant-workflow">SysQuant </a>phosphoproteomics</p> </td> <td style="width: 24.4771%;">Generally not applicable</td> <td style="width: 24.4771%;">600ul</td> <td style="width: 24.4771%;">7.4 mg</td> </tr> </tbody> </table> <p>Case studies:</p> <ol style="list-style-type: lower-alpha;"> <li><em>“Comprehensive Quantitative Profiling of Tau and Phosphorylated Tau Peptides in Cerebrospinal Fluid by Mass Spectrometry Provides New Biomarker Candidates”</em>. A Proteome Sciences paper co-authored with University of Gothenburg. Combined use of a brain trigger and phosphopeptide enrichment alongside standard proteomics to identify several thousand proteins and unique phosphorylation events related to Alzheimer’s disease biology that can be monitored in cerebrospinal fluid (CSF). This paper includes the first report of quantification for over 30 phosphorylation sites on Tau in Alzheimer’s disease and control individual CSF. </li> <li><em>“Combined tissue and fluid proteomics with Tandem Mass Tags® to identify low-abundance protein biomarkers of disease in peripheral body fluid: An Alzheimer’s Disease case study”</em>. A Proteome Sciences authored paper co-authored with University of Gothenburg and University College London describing application of the TMTcalibrator approach to identify biomarkers of neuroinflammation through use of cultured microglial cells as the trigger in a human CSF cohort. Also demonstrates translational potential as mouse BV2 cells were used in this case.</li> <li><em>“A Novel Method For Discovery of Peripheral Blood Biomarkers in Idiopathic Pulmonary Fibrosis Using Extensive Depletion and TMTcalibrator™ Tissue-Enhanced Plasma Proteomics”</em> A Proteome Sciences poster presentation co-authored with Pliant Therapeutics showing the powerful combination of combining a fibrotic lung tissue trigger and <a href="/services/discovery-based-proteomics/super-depletion">Super Depletion</a> of abundant plasma proteins to quantify over 5,600 proteins in plasma, including analysis of post-translationally modified collagens.</li> </ol> <p>An example report is available upon request.</p> <p>For FAQ’s on Unbiased Protein Biomarker Discovery based Proteomics please follow this link.</p> <table class="Table--themed" style="border-collapse: collapse; width: 99.8727%;" border="1"> <tbody> <tr> <td style="width: 49.1396%;">What’s included</td> <td style="width: 49.1396%;"> <ul> <li>Incoming sample QC to check sufficient protein in samples for a satisfactory analysis</li> <li>Protein isolation, peptide cleavage and TMT labelling</li> <li>Mass spectrometry-based peptide and protein ID</li> <li>Computational proteomics and full bioinformatics analysis</li> <li>Comprehensive report summarising the proteins, peptides and if applicable the peptide-PTM’s. Data interpretation, pathway analysis and biological relevance</li> <li>An Excel based QuantSheet detailing qualitative and quantitative protein identification data.</li> <li>Provision of all raw data if required</li> <li><a href="/contact?regards=request%20an%20example%20copy%20of%20our%20final%20analysis%20report">Contact us for an example copy of our final analysis report.</a></li> </ul> </td> </tr> <tr> <td style="width: 49.1396%;">Material required</td> <td style="width: 49.1396%;"> <ul> <li>This is sample and assay specific and will be detailed in the agreed Statement of Work - please <a href="/contact">contact us</a> to discuss your specific needs so we can start the Statement of Work preparation</li> </ul> </td> </tr> <tr> <td style="width: 49.1396%;">Typical turnaround time</td> <td style="width: 49.1396%;"> <ul> <li>Generally 6 to 8 weeks from receipt of up to 36 samples and submission of the comprehensive analytical report and associated data.</li> </ul> </td> </tr> </tbody> </table> </div> </div> </main> <footer class="Footer"> <div class="Footer-upper"> <div class="Container"> <div class="Grid Grid--fit Grid--alignMiddle"> <div class="Grid-cell u-sizeAuto u-lg-hide"> <a href="/" title="Back to home page"> <img class="Footer-logo" src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/images/logo-white.1654711795.svg" alt="Proteome Sciences"> </a> </div> <div class="Grid-cell u-lg-sizeFull u-lg-textCenter"> <ul class="FooterNav"> <li class="FooterNav-item is-first"> <a href="/about" class="is-first FooterNav-link">About Us</a> </li> <li class="FooterNav-item is-current"> <a href="/services" class="is-current FooterNav-link">Proteomic Services</a> </li> <li class="FooterNav-item"> <a href="/news" class="FooterNav-link">News</a> </li> <li class="FooterNav-item"> <a href="/investors" class="FooterNav-link">Investors</a> </li> <li class="FooterNav-item"> <a href="/contact" class="FooterNav-link">Contact</a> </li> <li class="FooterNav-item is-last"> <a href="/privacy-notice" class="is-last FooterNav-link">Privacy Notice</a> </li> </ul> </div> <div class="Grid-cell u-sizeAuto u-lg-size1of2 u-textRight u-lg-textCenter u-sm-sizeFull u-sm-margBottom4"> <img src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/images/ISO-9001-2.2015.jpg" height="125" width="150" alt="DQS Quality Management" class="Footer-badge"> <a href="https://twitter.com/ProteomeScience" title="Follow us on Twitter" class="Footer-iconLink" target="_blank" rel="noopenner noreferrer"> <svg class="Footer-icon Icon" role="img" alt="Twitter" aria-labelledby="iconTitle-b9Jd5w" focusable="false"><title id="iconTitle-b9Jd5w">Twitter</title><use xlink:href="#icon-twitter" /></svg></a> <a href="https://www.linkedin.com/company/proteome-sciences-plc" title="Follow us on LinkedIn" class="Footer-iconLink" target="_blank" rel="noopenner noreferrer"> <svg class="Footer-icon Icon" role="img" alt="LinkedIn" aria-labelledby="iconTitle-AaYUSc" focusable="false"><title id="iconTitle-AaYUSc">LinkedIn</title><use xlink:href="#icon-linkedin" /></svg></a> </div> </div> </div> </div> <div class="Container u-spaced1 u-gutters1"> <div class="Grid Grid--fit Grid--alignMiddle"> <div class="Grid-cell u-sizeAuto u-md-size1of2 u-md-textCenter u-sm-sizeFull u-sm-margBottom1 u-darkerText">Copyright © Proteome Sciences 2024</div> <div class="Grid-cell u-md-size1of2 u-md-textCenter u-sm-sizeFull u-darkerText"></div> <div class="Grid-cell u-sizeAuto u-md-sizeFull u-md-textCenter"> <div class="Footer-credit u-inlineBlock"> <a href="https://www.aptuitiv.com" target="_blank" class="Footer-link Footer-creditLink">Site Design By Aptuitiv</a>-<a href="https://www.branchcms.com" target="_blank" class="Footer-link Footer-creditLink">Powered by BranchCMS</a> </div> </div> </div> </div> </footer> <script> tarteaucitron.services.recaptchav3 = { "key": "recaptchav3", "type": "analytic", "name": "reCAPTCHA", "needConsent": true, "cookies": ['_GRECAPTCHA', 'AEC'], "js": function () { "use strict"; tarteaucitron.addScript('https://www.google.com/recaptcha/api.js?render=6Ld3p_obAAAAAD4LY-Z-EKeaaiTKbvoNLxpO_Vvh', '', function () { if(typeof grecaptcha === "undefined") {grecaptcha = {};}grecaptcha.ready = function(cb){if(typeof grecaptcha.execute === "undefined") {const c = "___grecaptcha_cfg";window[c] = window[c] || {};(window[c]["fns"] = window[c]["fns"]||[]).push(cb);} else {cb();}};grecaptcha.ready(function() {window.gCaptchaV3Promise8528 = function(grecaptcha, options) {options = options || {};if (typeof options.action === "undefined") {options.action = 'form'};if (typeof options.setField === "undefined") {options.setField = true;};if (typeof options.inputSelector === "undefined") {options.inputSelector = '.gRecaptchaV3nSMgAaxbgW';};return new Promise(function(resolve, reject) {grecaptcha.execute('6Ld3p_obAAAAAD4LY-Z-EKeaaiTKbvoNLxpO_Vvh', {action: options.action}).then(function(token) {if (options.setField) {document.querySelector(options.inputSelector).value = token;}resolve();}, function() {reject();});});};window.gCaptchaV3Promise8528(grecaptcha, {setField: false});}); }); } }; (tarteaucitron.job = tarteaucitron.job || []).push('recaptchav3'); </script> <script> // Change header styles to show gradient underneath the header document.querySelector('header').classList.add('Header--smScrHide'); </script> </body> </html>