Milo (P100) by ProteinSimple, Part of Bio-Techne

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<div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" title="Our capillary electrophoresis instruments (icIEF and CE-SDS) provide reproducible quantitative analysis for thorough therapeutic protein characterization." class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single-Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> <a href="/applications/crispr" class="dropdown-item text-wrap" 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data-drupal-link-system-path="node/1452">Organoid and 3-D Culture</a> <a href="/research-areas/stem-cells" class="dropdown-item text-wrap" data-drupal-link-system-path="node/161">Stem Cells</a> <a href="/research-areas/targeted-protein-degradation" class="dropdown-item text-wrap" data-drupal-link-system-path="node/376">Targeted Protein Degradation</a> <a href="/research-areas" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6741">View All Research Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col 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Proteins</a> <a href="/gmp-products/gmp-small-molecules" class="dropdown-item text-wrap" data-drupal-link-system-path="node/366">GMP Small Molecules</a> <a href="/gmp-products/gmp-antibodies" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13266">GMP Antibodies</a> <a href="/gmp-products/gmp-capabilities" class="dropdown-item text-wrap" title="GMP Capabilities" data-drupal-link-system-path="node/1071">GMP Capabilities</a> <a href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2066">Immuno-Oncology</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-diagnostics" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Diagnostics <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-diagnostics" aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1281">Custom Cell & Gene Therapy Services</a> <a href="/services/custom-compound-library-service" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2126">Custom Compound Library Services</a> <a href="/services/custom-elisa-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/261">Custom ELISA Services</a> <a href="/services/gene-engineering-services-tcbuster" class="dropdown-item text-wrap" title="Gene Engineering Services: Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup Tool</a> <a href="/resources/rnascope-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/rnascope-citations">RNAscope Citations</a> <a href="/resources/simple-plex-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-plex-panel-builder">Simple Plex Panel Builder</a> <a href="/resources/simple-western-kit-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-kit-builder">Simple Western Kit Builder</a> <a href="/resources/simple-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-antibody-database">Simple Western Antibody Database</a> <a href="/resources/single-cell-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/single-cell-western-antibody-database">Single-Cell Western Antibody Database</a> <a href="/resources/spectra-viewer" class="dropdown-item text-wrap" 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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> Products </li> <li> <a href="/search?keywords=Single-Cell%20Western&category_attr=Single-Cell%20Western">Single-Cell Western</a> </li> <li> Milo (P100) </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div class="top_region"><div class="o___product-summary mb-3 border-bottom"><div id="pp-page-title-brand-wrapper"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Milo </h1> <h2 class="font-weight-normal h4"> ProteinSimple, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">P100</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="arena_link product-documents" rel="nofollow">Product Documents</a> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" alt="ProteinSimple, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> </div> <div class="row mb-4"><div class="col-xl-3 order-xl-first" id="image_wrapper"><div id="images_summary"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="proteinsimple"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.rndsystems.com/images/datasheets/p100_milo-system-starter-package-1372020145622.png" alt="Milo System - Single-Cell Westerns" title="Milo - Single-Cell Westerns Profile Heterogeneity | ProteinSimple" data-caption="Milo Single-Cell Western platform from ProteinSimple, a Bio-Techne brand, measures protein expression in thousands of single cells in a single run. Validate single-cell RNA-seq data with single-cell protein data. " data-badges="" data-application="N/A" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(6)</span> </div> </div> </div> <i data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-heterogeneity.png" data-alt="I need to profile heterogeneity in complex samples" title="I need to profile heterogeneity in complex samples" data-caption="Milo measures protein expression in ~1,000 single-cells in one 4 hour experiment so you can quantify expression heterogeneity of your target and the percentage of cells in your sample that are target positive." data-badges="" data-application="" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-milo-multiplexing.png" data-alt="I need multiplexing to detect multiple proteins at the single-cell level" title="I need multiplexing to detect multiple proteins at the single-cell level" data-caption="Simultaneously detect 12+ proteins in your sample, all at the single-cell level, using spectral and size-based multiplexing strategies. You can even strip & reprobe scWest chips up to 9 times for higher multiplexed studies!" data-badges="" data-application="Single Cell Western" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-jurkat-graphs.png" data-alt="I need to simplify my phospho-flow signaling studies" title="I need to simplify my phospho-flow signaling studies" data-caption="Eliminate the fixation and permeabilization steps of flow/FACS! Milo chemically lyses the cells captured on the scWest chip before analysis to gain access to intracellular and intranuclear compartments more easily than with flow cytometry. By lysing the cells, Milo can detect challenging proteins like transcription factors and even methylated histones! You can also use Milo's sizing step to separate out non-specific binding, which is more common with phospho-specific antibodies and cannot be resolved with flow." data-badges="" data-application="" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-proteins.png" data-alt="I need to detect proteins that lack good flow antibodies" title="I need to detect proteins that lack good flow antibodies" data-caption="Milo is an open platform so you can use the large commercial catalog of Western antibodies which is 10-100x larger than the flow/FACS catalog. Don't get stuck without a flow antibody against your target of interest again." data-badges="" data-application="" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-isoforms.png" data-alt="I want to measure protein isoform heterogeneity" title="I want to measure protein isoform heterogeneity" data-caption="Milo's molecular weight sizing step can resolve protein isoforms that differ in molecular weight, allowing you to measure how many cells in your sample express one isoform, the other isoform, or both isoforms. Try that with flow!" data-badges="" data-application="" data-width="250" data-height="250" class="none"></i> </div> </div> </article> </div> </div> <div class="col-xl-9 order-xl-first" id="key_benefits_buttoon_wrapper"> <h3>Single-Cell Westerns</h3> <article class="product-data-wrapper "> Milo™ is the world's first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies. Use Single-Cell Westerns to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique. </article> <div id="pp-orderblock-buttons"><div class="float-right mt-3"> <a class="btn a___button-primary m-1 mt-3 col-xs-4" href="/p/instruments/single-cell-western/request-quote?productName=Milo&catalogNumber=P100" target="_self"> Request Pricing </a> </div> </div> </div> <div class="col-xl-3" id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> <div><div id="instrument_meet_section"><div id="instrument_meet_section" class="top_region"> <div class="row"> <div class="col-md-6"> <h3 class="mb-2">Single-Cell your Western with Milo</h3> Protein expression measurements made at the population level often overlook significant differences in expression between individual cells and the existence of cell subpopulations. Milo combines single-cell resolution with the power and versatility of Westerns. Identify target expression heterogeneity, visualize cell subpopulations you can’t see with conventional western blots, simplify phospho-flow studies, and validate your single-cell RNA-seq data at the protein level. </div> <div class="col-md-6" > <div class="embed-responsive embed-responsive-16by9"> <iframe width="560" height="315" src="https://www.youtube.com/embed/oVHbizlGH_U" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen> </iframe> </div> </div> </div> </div> </div> </div> <div><div id="instrument_additional_section"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> </div> </div> </div> <div class="tab-content"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="about-milo-tab-tab" href="#about-milo-tab" data-toggle="tab" role="tab" aria-controls="region-4171591922717" aria-selected="true" >About Milo</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-tab-tab" href="#citations-tab" data-toggle="tab" role="tab" aria-controls="region-8961620056042" aria-selected="false" >Citations / Testimonials (4)</a> </li> </ul> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4" id="about-milo-tab" data-tab-label="About Milo" role="tabpanel" aria-labelledby="about-milo-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="how-can-milo-help-you_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#how-can-milo-help-you" id="how-can-milo-help-you_li" >How Can Milo Help You?</a> </li> <li id="how-does-milo-work_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#how-does-milo-work" id="how-does-milo-work_li" >How Does Milo Work?</a> </li> <li id="applications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#applications" id="applications_li" >Applications</a> </li> <li id="products-compatible-with-milo_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#products-compatible-with-milo" id="products-compatible-with-milo_li" >Products Compatible with Milo</a> </li> <li id="specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#specifications" id="specifications_li" >Specifications</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents" href="#product-documents" id="product-documents_li" >Product Documents</a> </li> </div> <div id="how-can-milo-help-you" class="nav-link px-0" data-anchor-label="How Can Milo Help You?"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>How Can Milo Help You?</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-heterogeneity.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-heterogeneity.png" alt="I need to profile heterogeneity in complex samples" title="I need to profile heterogeneity in complex samples" data-caption="Milo measures protein expression in ~1,000 single-cells in one 4 hour experiment so you can quantify expression heterogeneity of your target and the percentage of cells in your sample that are target positive." data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need to profile heterogeneity in complex samples</h4> <p> Milo measures protein expression in ~1,000 single-cells in one 4 hour experiment so you can quantify expression heterogeneity of your target and the percentage of cells in your sample that are target positive. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-protein-validation.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-protein-validation.png" alt="I need protein validation of my single-cell RNA-seq data" title="I need protein validation of my single-cell RNA-seq data" data-caption="Use conventional western antibodies to validate your single-cell RNA-seq data with single-cell protein data. Plus, scWest chips can be archived for up to 9 months after you run them so you have plenty of time to get your sequencing results back before you have to probe for your targets of interest." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need protein validation of my single-cell RNA-seq data</h4> <p> Use conventional western antibodies to validate your single-cell RNA-seq data with single-cell protein data. Plus, scWest chips can be archived for up to 9 months after you run them so you have plenty of time to get your sequencing results back before you have to probe for your targets of interest. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-milo-multiplexing.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-milo-multiplexing.png" alt="I need multiplexing to detect multiple proteins at the single-cell level" title="I need multiplexing to detect multiple proteins at the single-cell level" data-caption="Simultaneously detect 12+ proteins in your sample, all at the single-cell level, using spectral and size-based multiplexing strategies. You can even strip & reprobe scWest chips up to 9 times for higher multiplexed studies!" data-badges="" data-application="Single Cell Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need multiplexing to detect multiple proteins at the single-cell level</h4> <p> Simultaneously detect 12+ proteins in your sample, all at the single-cell level, using spectral and size-based multiplexing strategies. You can even strip & reprobe scWest chips up to 9 times for higher multiplexed studies! </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-jurkat-graphs.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-jurkat-graphs.png" alt="I need to simplify my phospho-flow signaling studies" title="I need to simplify my phospho-flow signaling studies" data-caption="Eliminate the fixation and permeabilization steps of flow/FACS! Milo chemically lyses the cells captured on the scWest chip before analysis to gain access to intracellular and intranuclear compartments more easily than with flow cytometry. By lysing the cells, Milo can detect challenging proteins like transcription factors and even methylated histones! You can also use Milo's sizing step to separate out non-specific binding, which is more common with phospho-specific antibodies and cannot be resolved with flow." data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need to simplify my phospho-flow signaling studies</h4> <p> Eliminate the fixation and permeabilization steps of flow/FACS! Milo chemically lyses the cells captured on the scWest chip before analysis to gain access to intracellular and intranuclear compartments more easily than with flow cytometry. By lysing the cells, Milo can detect challenging proteins like transcription factors and even methylated histones! You can also use Milo's sizing step to separate out non-specific binding, which is more common with phospho-specific antibodies and cannot be resolved with flow. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-proteins.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-proteins.png" alt="I need to detect proteins that lack good flow antibodies" title="I need to detect proteins that lack good flow antibodies" data-caption="Milo is an open platform so you can use the large commercial catalog of Western antibodies which is 10-100x larger than the flow/FACS catalog. Don't get stuck without a flow antibody against your target of interest again." data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need to detect proteins that lack good flow antibodies</h4> <p> Milo is an open platform so you can use the large commercial catalog of Western antibodies which is 10-100x larger than the flow/FACS catalog. Don't get stuck without a flow antibody against your target of interest again. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-isoforms.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-isoforms.png" alt="I want to measure protein isoform heterogeneity" title="I want to measure protein isoform heterogeneity" data-caption="Milo's molecular weight sizing step can resolve protein isoforms that differ in molecular weight, allowing you to measure how many cells in your sample express one isoform, the other isoform, or both isoforms. Try that with flow!" data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I want to measure protein isoform heterogeneity</h4> <p> Milo's molecular weight sizing step can resolve protein isoforms that differ in molecular weight, allowing you to measure how many cells in your sample express one isoform, the other isoform, or both isoforms. Try that with flow! </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-gene-editing-efficiency.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-gene-editing-efficiency.png" alt="I need to measure gene editing efficiency or effect in low efficiency gene editing systems" title="I need to measure gene editing efficiency or effect in low efficiency gene editing systems" data-caption="Milo can quantify the percentage of cells in your sample that express an inserted gene, even in low efficiency systems. Plus, you can simultaneously measure your edited gene and downstream effect markers to understand the impact of the gene on the cells that have been edited." data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need to measure gene editing efficiency or effect in low efficiency gene editing systems</h4> <p> Milo can quantify the percentage of cells in your sample that express an inserted gene, even in low efficiency systems. Plus, you can simultaneously measure your edited gene and downstream effect markers to understand the impact of the gene on the cells that have been edited. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-expression-low-abundance.png" data-srcset="https://resources.rndsystems.com/images/datasheets/P100_single-cell-western-milo-scw-expression-low-abundance.png" alt="I need to measure protein expression in low abundance samples" title="I need to measure protein expression in low abundance samples" data-caption="Milo can analyze samples which contain as low as 10,000 single-cells so you no longer have to have collect millions of cells to analyze protein expression. The number of cells captured scales with the number of cells loaded so even lower abundance samples are possible. Milo can also characterize highly enriched FACS-sorted cell populations which don't contain enough cells to analyze with other techniques." data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>I need to measure protein expression in low abundance samples</h4> <p> Milo can analyze samples which contain as low as 10,000 single-cells so you no longer have to have collect millions of cells to analyze protein expression. The number of cells captured scales with the number of cells loaded so even lower abundance samples are possible. Milo can also characterize highly enriched FACS-sorted cell populations which don't contain enough cells to analyze with other techniques. </p> </td> </tr> </article> </table> </div> <div id="how_does_work-anchor" class="nav-link static-anchor p-0" data-anchor-label="How Does Milo Work?"></div> <div id="applications-anchor" class="nav-link static-anchor p-0" data-anchor-label="Applications"></div> <div id="products_compatible-anchor" class="nav-link static-anchor p-0" data-anchor-label="Products Compatible with Milo"></div> <div id="specifications-anchor" class="nav-link static-anchor p-0" data-anchor-label="Specifications"></div> <div id="product-datasheet-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Documents"></div> </div> </div> <div class="tab-pane fade mx-4 py-4" id="citations-tab" data-tab-label="Citations / Testimonials (4)" role="tabpanel" aria-labelledby="citations-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#testimonials"> Testimonials </a> </li> </div> </div> <div id="citations"> <div class="pp_filter_list border-bottom mb-4 pb-3"> <h2>Citations for Milo</h2> <p><a href="/resources/instrument-citations?instrument-platform=single-cell%20western" rel="nofollow noopener noreferrer" target="_blank">View all citations for the Single-Cell Western platform.</a><br> Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. </p> </div> </div> <div class="testimonials" id="testimonials"><article class="product-data-wrapper"> <div class="pp_filter_list mt-4 pt-4 border-bottom mb-4 pb-3"> <h2>Testimonials from your Peers for Single-Cell Western</h2> <div class="testimonials_showing pp_filters_showing row col-md-12 mb-2"> <span class="less">Showing  <span class="testimonials_show_min font-weight-bold">1</span> <span class="font-weight-bold">-</span> <span class="testimonials_show_max font-weight-bold">4</span> of <span class="testimonials_count font-weight-bold">4</span> testimonials </span> <span class="more font-weight-bold">Showing All</span> </div> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-prashant.png" alt="Prashant Vijay Thakkar Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/milo-taxane-resistance-at-the-single-cell-level">Prashant Gets a Better Picture of Taxane Resistance at the Single-Cell Level with Milo</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Prashant Vijay Thakkar, Ph.D., </span> Postdoctoral associate, Department of Medicine, Weill Cornell Medicine </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-yi-zhong.png" alt="Yi Zhong Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Milo permits study of proteins at the single-cell level to understand the heterogeneity among metastatic cells within a population."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/milo-heterogeneity-in-metastatic-cell-populations">Dr. Zhong & Colleagues Understand Heterogeneity in Metastatic Cell Populations with Milo</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Yi Zhong, M.D., Ph.D., </span> Research Associate, Memorial Sloan Kettering Cancer Center </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-maya-kaelberer.png" alt="M. Maya Kaelberer Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We have been pairing Milo with single-cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/milo-validates-neuroepithelial-circuit">Guts and Glory: Validating a Neuroepithelial Circuit using Milo</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> M. Maya Kaelberer, Ph.D., </span> Postdoctoral Associate, Duke University School of Medicine </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/milo-single-cell-western-chia-wei-cheng.png" alt="Chia-Wei Cheng, Ph.D." loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"With Milo, we observed a level of single-cell protein expression heterogeneity in our purified intestinal stem cell population that was not previously possible with conventional Western blotting."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/cheng-milo-goes-keto">Milo Goes Keto: Conquering Analysis of Scarce Intestinal Stem Cell Populations</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Chia-Wei Cheng, Ph.D., </span> Postdoctoral Fellow, Koch Institute for Integrative Cancer Research, MIT </p> </section> </div> </div> </article> <span id="more-testimonials" style="display:none"> </span> <div id="testimonial_counts" data-max="4" data-min="5"> <div class="testimonials_showing pp_filters_showing row col-md-12 mb-2"> <span class="less">Showing  <span class="testimonials_show_min font-weight-bold">1</span> <span class="font-weight-bold">-</span> <span class="testimonials_show_max font-weight-bold">4</span> of <span class="testimonials_count font-weight-bold">4</span> testimonials </span> <span class="more font-weight-bold">Showing All</span> </div> </div> <div class="text-center"> </div> </div> </article> </div> </div> </div> </div> <div><div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">More Information About Milo</h3> </div> <div class="vtab-section" data-section="How Does Milo Work?" id="how-does-milo-work"> <div class="card-header rounded-top"> <a id="how-does-milo-work-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-5781592502255" aria-expanded="false" aria-controls="collapse_region-5781592502255"> <h5 class="mb-0"> How Does Milo Work? </h5> </a> </div> <div id="collapse_region-5781592502255" class="collapse " aria-labelledby="heading_region-5781592502255" data-parent="#accordion"> <div class="card-body"> <div id="how-does-milo-work-vtab" data-tab-label="How Does Milo Work?" role="tabpanel" aria-labelledby="how-does-milo-work-vtab-tab"><div id="instrument_how_work_content"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> </div> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Applications" id="applications"> <div class="card-header rounded-top"> <a id="applications-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-4951592502294" aria-expanded="false" aria-controls="collapse_region-4951592502294"> <h5 class="mb-0"> Applications </h5> </a> </div> <div id="collapse_region-4951592502294" class="collapse " aria-labelledby="heading_region-4951592502294" data-parent="#accordion"> <div class="card-body"> <div id="applications-vtab" data-tab-label="Applications" role="tabpanel" aria-labelledby="applications-vtab-tab"><div id="applications_wrapper"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Milo Applications</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-cancerio-app-2.jpg" alt="Cancer & Immuno-Oncology" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > <h3 class="card_title_htag"> <div><h3>Cancer & Immuno-Oncology</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > Solutions for Cancer and IO </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-sarscov-2.jpg" alt="SARS-CoV-2" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research" > <h3 class="card_title_htag"> <div><h3>SARS-CoV-2</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research" > Solutions for SARS-CoV-2 Research </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/research-areas/cell-and-gene-therapy/analytical-solutions" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-cellgenetherapy-2.jpg" alt="Cell & Gene Therapy" loading="lazy" > </div> </a> <a class="card_title_link" href="/research-areas/cell-and-gene-therapy/analytical-solutions" > <h3 class="card_title_htag"> <div><h3>Cell & Gene Therapy</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.<br>  </p></div> </div> <div class="card-bottom-link"> <div> <a href="/research-areas/cell-and-gene-therapy/analytical-solutions" > Tools for C&GT </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-targeteddegradation-2.jpg" alt="Targeted Protein Degradation" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation" > <h3 class="card_title_htag"> <div><h3>Targeted Degradation</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation" > Solutions for TPD </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/sars-cov-2-multi-antigen-serology-module-simple-western" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-serology-2.jpg" alt="SARS-CoV-2 Serology" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/sars-cov-2-multi-antigen-serology-module-simple-western" > <h3 class="card_title_htag"> <div><h3>SARS-CoV-2 Serology</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/sars-cov-2-multi-antigen-serology-module-simple-western" > SARS-CoV-2 Serology Module </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-exosome-2.jpg" alt="Exosome" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess" > <h3 class="card_title_htag"> <div><h3>Exosome</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess" > Accelerate Exosome Research </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Products Compatible with Milo" id="products-compatible-with-milo"> <div class="card-header rounded-top"> <a id="products-compatible-with-milo-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-7641628092667" aria-expanded="false" aria-controls="collapse_region-7641628092667"> <h5 class="mb-0"> Products Compatible with Milo </h5> </a> </div> <div id="collapse_region-7641628092667" class="collapse " aria-labelledby="heading_region-7641628092667" data-parent="#accordion"> <div class="card-body"> <div id="products-compatible-with-milo-vtab" data-tab-label="Products Compatible with Milo" role="tabpanel" aria-labelledby="products-compatible-with-milo-vtab-tab"><div id="products_compatible_with_instrument"><article class="product-data-wrapper"> <ul class="compatible_instruments list-unstyled m-0"> <li class="py-2"><a href="/resources/single-cell-western-antibody-database">Milo Antibody Database</a></li> <li class="py-2"><a href="/search?keywords=Single-Cell%20Western%20Consumables&sub_category=Single-Cell%20Western%20Consumables&compatible_instrument=Milo&view=condensed">Single-Cell Western Consumables</a></li> <li class="py-2"><a href="/search?keywords=single-cell%20western&compatible_instrument=Milo&view=condensed">View All Products Compatible with Milo</a></li> </ul> </article> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Specifications" id="specifications"> <div class="card-header rounded-top"> <a id="specifications-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-9701592502350" aria-expanded="false" aria-controls="collapse_region-9701592502350"> <h5 class="mb-0"> Specifications </h5> </a> </div> <div id="collapse_region-9701592502350" class="collapse " aria-labelledby="heading_region-9701592502350" data-parent="#accordion"> <div class="card-body"> <div id="specifications-vtab" data-tab-label="Specifications" role="tabpanel" aria-labelledby="specifications-vtab-tab"><div id="instrument_specifications"><table class="tablesaw tablesaw-stack app_table my-3 table-bordered" data-tablesaw-mode="stack"> <thead class="ds_table_head bg-light-gray"> <th class="ds_table_head_cell w-25 p-3">Feature</th> <th class="ds_table_head_cell w-25 p-3">Description</th> </thead> <tbody class="bg-white"> <tr class="ds_table_row bg-light-gray-2"> <td class="text-left" colspan="2"> Requirements & Compatibility </td> </tr> <tr class="ds_table_row"> <td>Sample type</td> <td colspan="1" class="text-left"> Suspension containing >10,000 cells </td> </tr> <tr class="ds_table_row"> <td>Cell diameter</td> <td colspan="1" class="text-left"> 7-25 µm in suspension </td> </tr> <tr class="ds_table_row"> <td>Cell type</td> <td colspan="1" class="text-left"> Mammalian cells; globular in suspension and unfixed </td> </tr> <tr class="ds_table_row"> <td>Antibody requirement</td> <td colspan="1" class="text-left"> Standard unlabeled primaries and fluorescent secondaries </td> </tr> <tr class="ds_table_row"> <td>Other equipment needed</td> <td colspan="1" class="text-left"> Open-format fluorescence microarray scanner capable of 5 µm resolution </td> </tr> <tr class="ds_table_row bg-light-gray-2"> <td class="text-left" colspan="2"> Performance & Specifications </td> </tr> <tr class="ds_table_row"> <td>Typical cell dilutions</td> <td colspan="1" class="text-left"> Yield capture and analysis of 1,000-2,000 cells per scWest chip </td> </tr> <tr class="ds_table_row"> <td>Molecular weight (MW) range</td> <td colspan="1" class="text-left"> 15-175 kDa </td> </tr> <tr class="ds_table_row"> <td>MW resolution</td> <td colspan="1" class="text-left"> 10% differences in distinct spectral channels, as low as 30% differences in same spectral channel </td> </tr> <tr class="ds_table_row"> <td>Typical target multiplexing</td> <td colspan="1" class="text-left"> Up to four proteins per cell by spectral and size-based multiplexing Twelve - plus proteins per cell using stripping & reprobing </td> </tr> <tr class="ds_table_row"> <td>Workflow time</td> <td colspan="1" class="text-left"> 4-6 hours </td> </tr> </tbody> </table> </div> </div> </div> </div> </div> </div> </div> <div id="product-documents"> <div id="accordion-arena-documents" class="accordion border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">Product Documents for Milo</h3> </div> <div class="accordion-item vtab-section"> <div class="card-header rounded-top" id="document-heading1"> <a class="collapsed arena-document-title" id="product-documents-link" data-toggle="collapse" data-target="#document-collapse1" aria-expanded="false" aria-controls="document-collapse1"> <h5>Brochure</h5> </a> </div> <div id="document-collapse1" class="collapse" aria-labelledby="document-heading1" data-parent="#accordion-arena-documents"> <div class="card-body"> <div class="row"> <div class="col-md-6"> <div id="arena-document-pl6-0024"> <a href="/" class="arena_link download_arena_document" data-category_id="26" data-document_id="pl6-0024" data-document_type="brochure" target="_blank" rel="nofollow">Download STRY0200525 WBU BR Single-Cell-Westerns-Milo MF-v9</a> </div> </div> <div class="col-md-6 datasheet-pdf-result"></div> </div> </div> </div> </div> <div class="accordion-item vtab-section"> <div class="card-header rounded-top" id="document-heading2"> <a class="collapsed arena-document-title" data-toggle="collapse" data-target="#document-collapse2" aria-expanded="false" aria-controls="document-collapse2"> <h5>Protocols & Manuals</h5> </a> </div> <div id="document-collapse2" class="collapse" aria-labelledby="document-heading2" data-parent="#accordion-arena-documents"> <div class="card-body"> <div class="row"> <div class="col-md-6"> <div id="arena-document-035-057"> <div class="arena-document-placeholder spinner-border spinner-border-sm mt-3 ml-3" role="status" data-document_id="035-057" data-document_type="user manual" data-category_id="26"></div> Loading product document information... </div> </div> <div class="col-md-6 datasheet-pdf-result"></div> </div> </div> </div> </div> </div> </div> <div id="product-page-details" data-time-Total="0" data-time-ToCompile="0" data-time-ToBuild="0" data-time-Datapoints="0" data-time-Template="0" data-time-Post-EPCC="0" data-time-EPCC="0" data-renderTime="Saturday 23rd of November 2024 07:07:49 AM" 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Milo (P100) by ProteinSimple, Part of Bio-Techne