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promoting rhizobacteria</title> <meta name="description" content="Search results for: plant growth promoting rhizobacteria"> <meta name="keywords" content="plant growth promoting rhizobacteria"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research 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method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="plant growth promoting rhizobacteria"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 10458</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: plant growth promoting rhizobacteria</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10458</span> Biocontrol Potential of Growth Promoting Rhizobacteria against Root Rot of Chili and Enhancement of Plant Growth</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kiran%20Nawaz">Kiran Nawaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Waheed%20Anwar"> Waheed Anwar</a>, <a href="https://publications.waset.org/abstracts/search?q=Sehrish%20Iftikhar"> Sehrish Iftikhar</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Nasir%20Subhani"> Muhammad Nasir Subhani</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20Ali%20Shahid"> Ahmad Ali Shahid</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant growth promoting rhizobacteria (PGPR) have been extensively studied and applied for the biocontrol of many soilborne diseases. These rhizobacteria are very efficient against root rot and many other foliar diseases associated with solanaceous plants. These bacteria may inhibit the growth of various pathogens through direct inhibition of target pathogens or indirectly by the initiation of systemic resistance (ISR) which is active all over the complete plant. In the present study, 20 different rhizobacterial isolates were recovered from the root zone of healthy chili plants. All soil samples were collected from various chili-growing areas in Punjab. All isolated rhizobacteria species were evaluated in vitro and in vivo against Phytophthora capsici. Different species of Bacillus and Pseudomonas were tested for the antifungal activity against P. capsici the causal organism of Root rot disease in different crops together with chili. Dual culture and distance culture bioassay were carried out to study the antifungal potential of volatile and diffusible metabolites secreted from rhizobacteria. After seven days of incubation at 22°C, growth inhibition rate was recorded. Growth inhibition rate depended greatly on the tested bacteria and screening methods used. For diffusible metabolites, inhibition rate was 35-62% and 20-45% for volatile metabolites. The screening assay for plant growth promoting and disease inhibition potential of chili associated PGPR indicated 42-100% reduction in disease severity and considerable enhancement in roots fresh weight by 55-87%, aerial parts fresh weight by 35-65% and plant height by 65-76% as compared to untreated control and pathogen-inoculated plants. Pseudomonas flourescene, B. thuringiensis, and B. subtilis were found to be the most efficient isolates in inhibiting P. capsici radial growth, increase plant growth and suppress disease severity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rhizobacteria" title="rhizobacteria">rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=chili" title=" chili"> chili</a>, <a href="https://publications.waset.org/abstracts/search?q=phytophthora" title=" phytophthora"> phytophthora</a>, <a href="https://publications.waset.org/abstracts/search?q=root%20rot" title=" root rot"> root rot</a> </p> <a href="https://publications.waset.org/abstracts/66513/biocontrol-potential-of-growth-promoting-rhizobacteria-against-root-rot-of-chili-and-enhancement-of-plant-growth" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66513.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">263</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10457</span> Alleviation of Thermal Stress in Pinus ponderosa by Plant-Growth Promoting Rhizobacteria Isolated from Mixed-Conifer Forests</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kelli%20G.%20Thorup">Kelli G. Thorup</a>, <a href="https://publications.waset.org/abstracts/search?q=Kristopher%20A.%20Blee"> Kristopher A. Blee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Climate change enhances the occurrence of extreme weather: wildfires, drought, rising summer temperatures, all of which dramatically decline forest growth and increase tree mortality in the mixed-conifer forests of Sierra Nevada, California. However, microbiota living in mutualistic relations with plant rhizospheres have been found to mitigate the effects of suboptimal environmental conditions. The goal of this research is to isolate native beneficial bacteria, plant-growth promoting rhizobacteria (PGPR), that can alleviate heat stress in Pinus ponderosa seedlings. Bacteria were isolated from the rhizosphere of Pinus ponderosa juveniles located in mixed-conifer stand and further characterized for PGP potential based on their ability to produce key growth regulatory phytohormones including auxin, cytokinin, and gibberellic acid. Out of ten soil samples taken, sixteen colonies were isolated and qualitatively confirmed to produce indole-3-acetic acid (auxin) using Salkowski’s reagent. Future testing will be conducted to quantitatively assess phytohormone production in bacterial isolates. Furthermore, bioassays will be performed to determine isolates abilities to increase tolerance in heat-stressed Pinus ponderosa seedlings. Upon completion of this research, a PGPR could be utilized to support the growth and transplantation of conifer seedlings as summer temperatures continue to rise due to the effects of climate change. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=conifer" title="conifer">conifer</a>, <a href="https://publications.waset.org/abstracts/search?q=heat-stressed" title=" heat-stressed"> heat-stressed</a>, <a href="https://publications.waset.org/abstracts/search?q=phytohormones" title=" phytohormones"> phytohormones</a>, <a href="https://publications.waset.org/abstracts/search?q=Pinus%20ponderosa" title=" Pinus ponderosa"> Pinus ponderosa</a>, <a href="https://publications.waset.org/abstracts/search?q=plant-growth%20promoting%20rhizobacteria" title=" plant-growth promoting rhizobacteria"> plant-growth promoting rhizobacteria</a> </p> <a href="https://publications.waset.org/abstracts/135826/alleviation-of-thermal-stress-in-pinus-ponderosa-by-plant-growth-promoting-rhizobacteria-isolated-from-mixed-conifer-forests" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/135826.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">118</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10456</span> Rejuvenation of Peanut Seedling from Collar Rot Disease by Azotobacter sp. RA2</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ravi%20R.%20Patel">Ravi R. Patel</a>, <a href="https://publications.waset.org/abstracts/search?q=Vasudev%20R.%20Thakkar"> Vasudev R. Thakkar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Use of plant growth-promoting rhizobacteria (PGPR) to increase the production and decrees disease occurrence is a recent method in agriculture. An RA2 rhizospheric culture was isolated from peanut rhizosphere from Junagadh region of Gujarat, India and showed different direct and indirect plant growth promoting activity like indole acetic acid, gibberellic acid, siderophore, hydrogen cyanide, Ammonia and (1-Aminocyclopropane-1-Carboxylate) deaminase production, N2 fixation, phosphate and potassium solubilization in vitro. RA2 was able to protect peanut germinating seedling from A. niger infection and reduce collar rot disease incidence 60-35% to 72-41% and increase germination percentage from 70-82% to 75-97% in two varieties GG20 and GG2 of peanut. RA2 was found to induce resistance in A. hypogaea L. seedlings via induction of different defense-related enzymes like phenylalanine ammonia lyase, peroxidase, polyphenol oxidase, lipoxygenase and pathogenesis related protein like chitinase, ß – 1,3- glucanase. Jasmonic acid one of the major signaling molecules of inducing systemic resistance was also found to induced due to RA2 treatments. RA2 bacterium was also promoting peanut growth and reduce A. niger infection in pot studies. 16S rDNA sequence of RA2 showed 99 % homology to Azotobacter species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20promoting%20rhizobacteria" title="plant growth promoting rhizobacteria">plant growth promoting rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=peanut" title=" peanut"> peanut</a>, <a href="https://publications.waset.org/abstracts/search?q=aspergillus%20niger" title=" aspergillus niger"> aspergillus niger</a>, <a href="https://publications.waset.org/abstracts/search?q=induce%20systemic%20resistance" title=" induce systemic resistance"> induce systemic resistance</a> </p> <a href="https://publications.waset.org/abstracts/59871/rejuvenation-of-peanut-seedling-from-collar-rot-disease-by-azotobacter-sp-ra2" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59871.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">242</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10455</span> Antifungal Potential of the Plant Growth-Promoting Rhizobacteria Infecting Kidney Beans</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhazira%20Shemsheyeva">Zhazira Shemsheyeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhanara%20Suleimenova"> Zhanara Suleimenova</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Shemshura"> Olga Shemshura</a>, <a href="https://publications.waset.org/abstracts/search?q=Gulnaz%20Mombekova"> Gulnaz Mombekova</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhanar%20Rakhmetova"> Zhanar Rakhmetova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacteria that colonize plant roots and promote plant growth are referred to as plant growth-promoting rhizobacteria (PGPR). They not only provide nutrients to the plants (direct plant growth promotion) and protect plants against the phytopathogens (indirect plant growth promotion) but also increase the soil fertility. Indirectly PGPRs improve the plant growth by becoming a biocontrol agent for a fungal pathogen. The antifungal activities of the PGPrhizobacteria were assayed against different species of phytopathogenic fungi such as Fusarium tricinctum, Fusarium oxysporum, Sclerotiniasclerotiorum, and Botrytis cinerea. Pseudomonas putidaSM-1, Azotobacter sp., and Bacillus thuringiensis AKS/16 strains have been used in experimental tests on growth inhibition of phytopathogenic fungi infecting Kidney beans. Agar well diffusion method was used in this study. Diameters of the zones of inhibition were measured in millimeters. It was found that Bacillus thuringiensis AKS/16 strain showed the lowest antifungal activity against all fungal pathogens tested. Zones of inhibition were 15-18 mm. In contrast, Pseudomonas putida SM-1 exhibited good antifungal activity against Fusarium oxysporum and Fusarium tricinctum by producing 29-30 mm clear zones of inhibition. The moderate inhibitory effect was shown by Azotobacter sp. against all fungal pathogens tested with zones of inhibition from24 to 26 mm. In summary, Pseudomonas putida SM-1 strain demonstrated the potential of controlling root rot diseases in kidney beans. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=PGPR" title="PGPR">PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=pseudomonas%20putida" title=" pseudomonas putida"> pseudomonas putida</a>, <a href="https://publications.waset.org/abstracts/search?q=kindey%20beans" title=" kindey beans"> kindey beans</a>, <a href="https://publications.waset.org/abstracts/search?q=antifungal%20activity" title=" antifungal activity "> antifungal activity </a> </p> <a href="https://publications.waset.org/abstracts/120943/antifungal-potential-of-the-plant-growth-promoting-rhizobacteria-infecting-kidney-beans" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/120943.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">154</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10454</span> Screening of Indigenous Rhizobacteria for Growth Promoting and Antagonistic Activity against Fusarium Oxysporoum in Tomato</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20H.%20Abu-Dieyeh">Mohammed H. Abu-Dieyeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20M.%20Zalloum"> Mohammad M. Zalloum</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant growth-promoting rhizobacteria (PGPR) are known to enhance plant growth and/or reduce plant damage due to soil-borne pathogens. Tomato is the highest consumable vegetable world-wide including Jordan. Fusarium oxysporum is a pathogen that causes well-known damages and losses to many vegetable crops including tomato. In this study, purification of 112 isolates of PGPR strains from rhizosphere environment of different regions in Jordan was accomplished. All bacterial isolates were In-vitro screened for antagonistic effects against F. oxysporum. The eleven most effective isolates that caused 30%-50% in-vitro growth reduction of F. oxysporum were selected. 8 out of 11 of these isolates were collected from Al-Halabat (arid-land). 7 isolates of Al-Halabat exerted 40-54% In-vitro growth reduction of F. oxysporum. Four-week-old seedlings of tomato cultivar (Anjara, the most susceptible indigenous cultivar to F. oxysporum) treated with PGPR5 (Bacillus amyloliquefaciens), and exposed to F. oxysporum, showed no disease symptoms and no significant changes in biomasses or chlorophyll contents indicating a non-direct mechanism of action of PGPR on tomato plants. However PGPR3 (Bacillus sp.), PGPR4 (Bacillus cereus), and PGPR38 (Paenibacillus sp.) treated plants or PGPR treated and exposed to F. oxysporum showed a significant increasing growth of shoot and root biomasses as well as chlorophyll contents of leaves compared to control untreated plants or plants exposed to the fungus without PGPR treatment. A significant increase in number of flowers per plant was also recorded in all PGPR treated plants. The characterization of rhizobacterial strains were accomplished using 16S rRNA gene sequence analysis in addition to microscopic characterization. Further research is necessary to explore the potentiality of other collected PGPR isolates on tomato plants in addition to investigate the efficacy of the identified isolates on other plant pathogens and then finding a proper and effective methods of formulation and application of the successful isolates on selected crops. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antagonism" title="antagonism">antagonism</a>, <a href="https://publications.waset.org/abstracts/search?q=arid%20land" title=" arid land"> arid land</a>, <a href="https://publications.waset.org/abstracts/search?q=growth%20promoting" title=" growth promoting"> growth promoting</a>, <a href="https://publications.waset.org/abstracts/search?q=rhizobacteria" title=" rhizobacteria"> rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=tomato" title=" tomato"> tomato</a> </p> <a href="https://publications.waset.org/abstracts/30095/screening-of-indigenous-rhizobacteria-for-growth-promoting-and-antagonistic-activity-against-fusarium-oxysporoum-in-tomato" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30095.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">372</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10453</span> Effect of Chemical Mutagen on Seeds Germination of Lima Bean</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=G.%20Ultanbekova">G. Ultanbekova</a>, <a href="https://publications.waset.org/abstracts/search?q=Zh.%20Suleimenova"> Zh. Suleimenova</a>, <a href="https://publications.waset.org/abstracts/search?q=Zh.%20Rakhmetova"> Zh. Rakhmetova</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Mombekova"> G. Mombekova</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Mantieva"> S. Mantieva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant Growth Promoting Rhizobacteria (PGPR) are a group of free-living bacteria that colonize the rhizosphere, enhance plant growth of many cereals and other important agricultural crops and protect plants from disease and abiotic stresses through a wide variety of mechanisms. The use of PGPR has been proven to be an environmentally sound way of increasing crop yields by facilitating plant growth. In the present study, strain improvement of PGPR isolates were carried out by chemical mutagenesis for the improvement of growth and yield of lima bean. Induced mutagenesis is widely used for the selection of microorganisms producing biologically active substances and further improving their activities. Strain improvement is usually done by classical mutagenesis which involves exposing the microbes to chemical or physical mutagens. The strains of Pseudomonas putida 4/1, Azotobacter chroococcum Р-29 and Bacillus subtilis were subjected to mutation process for strain improvement by treatment with a chemical agent (sodium nitrite) to cause mutation and were observed for its consequent action on the seeds germination and plant growth of lima bean (Phaseolus lunatus). Bacterial mutant strains of Pseudomonas putida M-1, Azotobacter chroococcum M-1 and Bacillus subtilis M-1, treated with sodium nitrite in the concentration of 5 mg/ml for 120 min, were found effective to enhance the germination of lima bean seeds compared to parent strains. Moreover, treatment of the lima bean seeds with a mutant strain of Bacillus subtilis M-1 had a significant stimulation effect on plant growth. The length of the stems and roots of lima bean treated with Bacillus subtilis M-1 increased significantly in comparison with parent strain in 1.6 and 1.3 times, respectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chemical%20mutagenesis" title="chemical mutagenesis">chemical mutagenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=germination" title=" germination"> germination</a>, <a href="https://publications.waset.org/abstracts/search?q=kidney%20bean" title=" kidney bean"> kidney bean</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20promoting%20rhizobacteria%20%28PGPR%29" title=" plant growth promoting rhizobacteria (PGPR)"> plant growth promoting rhizobacteria (PGPR)</a> </p> <a href="https://publications.waset.org/abstracts/100013/effect-of-chemical-mutagen-on-seeds-germination-of-lima-bean" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/100013.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">198</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10452</span> Effect of Plant Growth Promoting Rhizobacteria on the Germination and Early Growth of Onion (Allium cepa)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dragana%20R.%20Stamenov">Dragana R. Stamenov</a>, <a href="https://publications.waset.org/abstracts/search?q=Simonida%20S.%20Djuric"> Simonida S. Djuric</a>, <a href="https://publications.waset.org/abstracts/search?q=Timea%20Hajnal%20Jafari"> Timea Hajnal Jafari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant growth promoting rhizobacteria (PGPR) are a heterogeneous group of bacteria that can be found in the rhizosphere, at root surfaces and in association with roots, enhancing the growth of the plant either directly and/or indirectly. Increased crop productivity associated with the presence of PGPR has been observed in a broad range of plant species, such as raspberry, chickpeas, legumes, cucumber, eggplant, pea, pepper, radish, tobacco, tomato, lettuce, carrot, corn, cotton, millet, bean, cocoa, etc. However, until now there has not been much research about influences of the PGPR on the growth and yield of onion. Onion (Allium cepa L.), of the Liliaceae family, is a species of great economic importance, widely cultivated all over the world. The aim of this research was to examine the influence of plant growth promoting bacteria Pseudomonas sp. Dragana, Pseudomonas sp. Kiš, Bacillus subtillis and Azotobacter sp. on the seed germination and early growth of onion (Allium cepa). PGPR Azotobacter sp., Bacillus subtilis, Pseudomonas sp. Dragana, Pseudomonas sp. Kiš, from the collection of the Faculty of Agriculture, Novi Sad, Serbia, were used as inoculants. The number of cells in 1 ml of the inoculum was 10⁸ CFU/ml. The control variant was not inoculated. The effect of PGPR on seed germination and hypocotyls length of Allium cepa was evaluated in controlled conditions, on filter paper in the dark at 22°C, while effect on the plant length and mass in semicontrol conditions, in 10 l volume vegetative pots. Seed treated with fungicide and untreated seed were used. After seven days the percentage of germination was determined. After seven and fourteen days hypocotil length was measured. Fourteen days after germination, length and mass of plants were measured. Application of Pseudomonas sp. Dragana and Kiš and Bacillus subtillis had a negative effect on onion seed germination, while the use of Azotobacter sp. gave positive results. On average, application of all investigated inoculants had a positive effect on the measured parameters of plant growth. Azotobacter sp. had the greatest effect on the hypocotyls length, length and mass of the plant. In average, better results were achieved with untreated seeds in compare with treated. Results of this study have shown that PGPR can be used in the production of onion. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=germination" title="germination">germination</a>, <a href="https://publications.waset.org/abstracts/search?q=length" title=" length"> length</a>, <a href="https://publications.waset.org/abstracts/search?q=mass" title=" mass"> mass</a>, <a href="https://publications.waset.org/abstracts/search?q=microorganisms" title=" microorganisms"> microorganisms</a>, <a href="https://publications.waset.org/abstracts/search?q=onion" title=" onion"> onion</a> </p> <a href="https://publications.waset.org/abstracts/80321/effect-of-plant-growth-promoting-rhizobacteria-on-the-germination-and-early-growth-of-onion-allium-cepa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80321.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">237</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10451</span> Screening of Plant Growth Promoting Rhizobacteria in the Rhizo- and Endosphere of Sunflower (Helianthus anus) and Their Role in Enhancing Growth and Yield Attriburing Trairs and Colonization Studies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Majeed">A. Majeed</a>, <a href="https://publications.waset.org/abstracts/search?q=M.K.%20Abbasi"> M.K. Abbasi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Hameed"> S. Hameed</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Imran"> A. Imran</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Naqqash"> T. Naqqash</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20K.%20Hanif"> M. K. Hanif</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant growth-promoting rhizobacteria (PGPR) are free-living soil bacteria that aggressively colonize the rhizosphere/plant roots, and enhance the growth and yield of plants when applied to seed or crops. Root associated (endophytic and rhizospheric) PGPR were isolated from Sunflower (Helianthus anus) grown in soils collected from 16 different sites of sub division Dhirkot, Poonch, Azad Jammu & Kashmir, Pakistan. A total of 150 bacterial isolates were isolated, purified, screened in vitro for their plant growth promoting (PGP) characteristics. 11 most effective isolates were selected on the basis of biochemical assays (nitrogen fixation, phosphate solubilization, growth hormone production, biocontrol assay, and carbon substrates utilization assay through gas chromatography (GCMS), spectrophotometry, high performance liquid chromatography HPLC, fungal and bacterial dual plate assay and BIOLOG GN2/GP2 microplate assay respectively) and were tested on the crop under controlled and field conditions. From the inoculation assay, the most promising 4 strains (on the basis of increased root/shoot weight, root/shoot length, seed oil content, and seed yield) were than selected for colonization studies through confocal laser scanning and transmission electron microscope. 16Sr RNA gene analysis showed that these bacterial isolates belong to Pseudononas, Enterobacter, Azospirrilum, and Citobacter genera. This study is the clear evident that such isolates have the potential for application as inoculants adapted to poor soils and local crops to minimize the chemical fertilizers harmful for soil and environment <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=PGPR" title="PGPR">PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrogen%20fixation" title=" nitrogen fixation"> nitrogen fixation</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphate%20solubilization" title=" phosphate solubilization"> phosphate solubilization</a>, <a href="https://publications.waset.org/abstracts/search?q=colonization" title=" colonization"> colonization</a> </p> <a href="https://publications.waset.org/abstracts/42869/screening-of-plant-growth-promoting-rhizobacteria-in-the-rhizo-and-endosphere-of-sunflower-helianthus-anus-and-their-role-in-enhancing-growth-and-yield-attriburing-trairs-and-colonization-studies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42869.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">340</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10450</span> Screening and Evaluation of Plant Growth Promoting Rhizobacteria of Wheat/Faba Bean for Increasing Productivity and Yield</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yasir%20Arafat">Yasir Arafat</a>, <a href="https://publications.waset.org/abstracts/search?q=Asma%20Shah"> Asma Shah</a>, <a href="https://publications.waset.org/abstracts/search?q=Hua%20Shao"> Hua Shao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and Aims: Legume/cereal intercropping is used worldwide for enhancement in biomass and yield of cereal crops. However, because of intercropping, the belowground biological and chemical interactions and their effect on physiological parameters and yield of crops are limited. Methods: Wheat faba bean (WF) intercropping was designed to understand the underlying changes in the soil's chemical environment, soil microbial communities, and effect on growth and yield parameters. Experimental plots were established as having no root partition (NRP), semi-root partition (SRP), complete root partition (CRP), and their sole cropping (CK). Low molecular weight organic acids (LMWOAs) were determined by GC-MS, and high throughput sequencing of the 16S rRNA gene was carried out to screen microbial structure and composition in different root partitions of the WF intercropping system. Results: We show that intercropping induced a shift in the relative abundance of some genera of plant growth promoting rhizobacteria (PGPR) such as Allorhizobium, Neorhizobium, Pararhizobium, and Rhizobium species and resulted in better growth and yield performance of wheat. Moreover, as the plant's distance of wheat from faba beans decreased, the diversity of microbes increased, and a positive effect was observed on physiological traits and crop yield. Furthermore, an abundance and positive correlations of palmitic acid, arachidic acid, stearic acid, and 9-Octadecenoic with PGPR were recorded in the root zone of WF intercropping, which can play an important role in this facilitative mechanism of enhancing growth and yield of cereals. Conclusion: The two treatments clearly affected soil microbial and chemical composition, which can be reflected in growth and yield enhancement. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=intercropping" title="intercropping">intercropping</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20community" title=" microbial community"> microbial community</a>, <a href="https://publications.waset.org/abstracts/search?q=LMWOAs" title=" LMWOAs"> LMWOAs</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR" title=" PGPR"> PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20chemical%20environment" title=" soil chemical environment"> soil chemical environment</a> </p> <a href="https://publications.waset.org/abstracts/179034/screening-and-evaluation-of-plant-growth-promoting-rhizobacteria-of-wheatfaba-bean-for-increasing-productivity-and-yield" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179034.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">84</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10449</span> Molecular Interactions between Vicia Faba L. Cultivars and Plant Growth Promoting Rhizobacteria (PGPR), Utilized as Yield Enhancing 'Plant Probiotics'</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eleni%20Stefanidou">Eleni Stefanidou</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolaos%20Katsenios"> Nikolaos Katsenios</a>, <a href="https://publications.waset.org/abstracts/search?q=Ioanna%20Karamichali"> Ioanna Karamichali</a>, <a href="https://publications.waset.org/abstracts/search?q=Aspasia%20Efthimiadou"> Aspasia Efthimiadou</a>, <a href="https://publications.waset.org/abstracts/search?q=Panagiotis%20Madesis"> Panagiotis Madesis</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The excessive use of pesticides and fertilizers has significant environmental and human health-related negative effects. In the frame of the development of sustainable agriculture practices, especially in the context of extreme environmental changes (climate change), it is important to develop alternative practices to increase productivity and biotic and abiotic stress tolerance. Beneficial bacteria, such as symbiotic bacteria in legumes (rhizobia) and symbiotic or free-living Plant Growth Promoting Rhizobacteria (PGPR), which could act as "plant probiotics", can promote plant growth and significantly increase the resistance of crops under adverse environmental conditions. In this study, we explored the symbiotic relationships between Faba bean (Vicia faba L.) cultivars with different PGPR bacteria, aiming to identify the possible influence on yield and biotic-abiotic phytoprotection benefits. Transcriptomic analysis of root and whole plant samples was executed for two Vicia faba L. cultivars (Polikarpi and Solon) treated with selected PGPR bacteria (6 treatments: B. subtilis + Rhizobium-mixture, A. chroococcum + Rhizobium-mixture, B. subtilis, A. chroococcum and Rhizobium-mixture). Preliminary results indicate a significant yield (Seed weight and Total number of pods) increase in both varieties, ranging around 25%, in comparison to the control, especially for the Solon cultivar. The increase was observed for all treatments, with the B. subtilis + Rhizobium-mixture treatment being the highest performing. The correlation of the physiological and morphological data with the transcriptome analysis revealed molecular mechanisms and molecular targets underlying the observed yield increase, opening perspectives for the use of nitrogen-fixing bacteria as a natural, more ecological enhancer of legume crop productivity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plant%20probiotics" title="plant probiotics">plant probiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR" title=" PGPR"> PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=legumes" title=" legumes"> legumes</a>, <a href="https://publications.waset.org/abstracts/search?q=sustainable%20agriculture" title=" sustainable agriculture"> sustainable agriculture</a> </p> <a href="https://publications.waset.org/abstracts/175741/molecular-interactions-between-vicia-faba-l-cultivars-and-plant-growth-promoting-rhizobacteria-pgpr-utilized-as-yield-enhancing-plant-probiotics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175741.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">80</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10448</span> Effect of Chemical Fertilizer on Plant Growth-Promoting Rhizobacteria in Wheat</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tessa%20E.%20Reid">Tessa E. Reid</a>, <a href="https://publications.waset.org/abstracts/search?q=Vanessa%20N.%20Kavamura"> Vanessa N. Kavamura</a>, <a href="https://publications.waset.org/abstracts/search?q=Maider%20Abadie"> Maider Abadie</a>, <a href="https://publications.waset.org/abstracts/search?q=Adriana%20Torres-Ballesteros"> Adriana Torres-Ballesteros</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Pawlett"> Mark Pawlett</a>, <a href="https://publications.waset.org/abstracts/search?q=Ian%20M.%20Clark"> Ian M. Clark</a>, <a href="https://publications.waset.org/abstracts/search?q=Jim%20Harris"> Jim Harris</a>, <a href="https://publications.waset.org/abstracts/search?q=Tim%20Mauchline"> Tim Mauchline</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The deleterious effect of chemical fertilizer on rhizobacterial diversity has been well documented using 16S rRNA gene amplicon sequencing and predictive metagenomics. Biofertilization is a cost-effective and sustainable alternative; improving strategies depends on isolating beneficial soil microorganisms. Although culturing is widespread in biofertilization, it is unknown whether the composition of cultured isolates closely mirrors native beneficial rhizobacterial populations. This study aimed to determine the relative abundance of culturable plant growth-promoting rhizobacteria (PGPR) isolates within total soil DNA and how potential PGPR populations respond to chemical fertilization in a commercial wheat variety. It was hypothesized that PGPR will be reduced in fertilized relative to unfertilized wheat. Triticum aestivum cv. Cadenza seeds were sown in a nutrient depleted agricultural soil in pots treated with and without nitrogen-phosphorous-potassium (NPK) fertilizer. Rhizosphere and rhizoplane samples were collected at flowering stage (10 weeks) and analyzed by culture-independent (amplicon sequence variance (ASV) analysis of total rhizobacterial DNA) and -dependent (isolation using growth media) techniques. Rhizosphere- and rhizoplane-derived microbiota culture collections were tested for plant growth-promoting traits using functional bioassays. In general, fertilizer addition decreased the proportion of nutrient-solubilizing bacteria (nitrate, phosphate, potassium, iron and, zinc) isolated from rhizocompartments in wheat, whereas salt tolerant bacteria were not affected. A PGPR database was created from isolate 16S rRNA gene sequences and searched against total soil DNA, revealing that 1.52% of total community ASVs were identified as culturable PGPR isolates. Bioassays identified a higher proportion of PGPR in non-fertilized samples (rhizosphere (49%) and rhizoplane (91%)) compared to fertilized samples (rhizosphere (21%) and rhizoplane (19%)) which constituted approximately 1.95% and 1.25% in non-fertilized and fertilized total community DNA, respectively. The analyses of 16S rRNA genes and deduced functional profiles provide an in-depth understanding of the responses of bacterial communities to fertilizer; this study suggests that rhizobacteria, which potentially benefit plants by mobilizing insoluble nutrients in soil, are reduced by chemical fertilizer addition. This knowledge will benefit the development of more targeted biofertilization strategies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteria" title="bacteria">bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=fertilizer" title=" fertilizer"> fertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=microbiome" title=" microbiome"> microbiome</a>, <a href="https://publications.waset.org/abstracts/search?q=rhizoplane" title=" rhizoplane"> rhizoplane</a>, <a href="https://publications.waset.org/abstracts/search?q=rhizosphere" title=" rhizosphere"> rhizosphere</a> </p> <a href="https://publications.waset.org/abstracts/132075/effect-of-chemical-fertilizer-on-plant-growth-promoting-rhizobacteria-in-wheat" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/132075.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">307</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10447</span> Nitrogen-Fixing Rhizobacteria (Rhizobium mililoti 2011) Enhances the Tolerance and the Accumulation of Cadmium in Medicago sativa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tahar%20Ghnaya">Tahar Ghnaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Majda%20Mnasri"> Majda Mnasri</a>, <a href="https://publications.waset.org/abstracts/search?q=Hanen%20Zaier"> Hanen Zaier</a>, <a href="https://publications.waset.org/abstracts/search?q=Rim%20Ghabriche"> Rim Ghabriche</a>, <a href="https://publications.waset.org/abstracts/search?q=Chedly%20Abdelly"> Chedly Abdelly</a> </p> <p class="card-text"><strong>Abstract:</strong></p> It is known that the symbiotic association between plant and microorganisms are beneficial for plant growth and resistance to metal stress. Hence, it was demonstrated that Arbuscular mycorrhizal fungi have a positive effect on host plants growing in metal polluted soils. Legume plants are those which normally associate to rhizobacteria in order to fix atmospheric nitrogen. The aim of this work was to evaluate the effect this type of symbiosis on the tolerance and the accumulation of Cd. We chose Medicago sativa, as a modal for host legume plants and Rhizobium mililoti 2011 as rhizobial strain. Inoculated and non-inoculated plants of M. sativa were submitted during three month to 0, 50, and 100 mgCd/kg dry soil. Results showed that the presence of Cd in the medium induced, in both inoculated and non-inoculated plants, a chlorosis and necrosis. However, these symptoms were more pronounced in non-inoculated plants. The beneficial effect of inoculation of M. sativa with R. meliloti, on plant growth was confirmed by the measurement of biomass production which showed that the symbiotic association between host plant and rhizobacteria alleviates significantly Cd effect on biomass production, so inoculated plants produced more dry weight as compared to non-inoculated ones in the presence of all Cd tretments. On the other hand, under symbiosis conditions, Cd was more accumulated in different plant organs. Hence, in these plants, shoot Cd concentration reached 425 and it was 280 µg/gDW in non-inoculated ones in the presence of 100 ppm Cd. This result suggests that symbiosis enhances the absorption and translocation of Cd in this plant. In nodules and roots, we detected the highest Cd concentrations, demonstrating that these organs are able to concentrate Cd in their tissues. These data confirm that M. sataiva, cultivated in symbiosis with Rhizobium mililoti could be used in phytoextraction of Cd from contaminated soils. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cd" title="Cd">Cd</a>, <a href="https://publications.waset.org/abstracts/search?q=phytoremediation" title=" phytoremediation"> phytoremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=Medicago%20sativa" title=" Medicago sativa"> Medicago sativa</a>, <a href="https://publications.waset.org/abstracts/search?q=Arbuscular%20mycorrhizal" title=" Arbuscular mycorrhizal"> Arbuscular mycorrhizal</a> </p> <a href="https://publications.waset.org/abstracts/21956/nitrogen-fixing-rhizobacteria-rhizobium-mililoti-2011-enhances-the-tolerance-and-the-accumulation-of-cadmium-in-medicago-sativa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21956.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">277</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10446</span> Biohydrogen Production from Rice Water Using Bacteria Isolated from Wetland Sediment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jerry%20John%20T.%20M.">Jerry John T. M.</a>, <a href="https://publications.waset.org/abstracts/search?q=Sylas%20V.%20P."> Sylas V. P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Shijo%20Joy"> Shijo Joy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hydrogen is the most essential gas that can be used for many purposes. During the production of hydrogen using raw materials like Soil and leftover cooked rice water (kanjivellam), the major by-product formed is water. Soil is collected from three different places in kottayam district: Kallara, Meenachilar, and Athirampuzha. Collected samples are mixed with rice water and tested for traces of hydrogen using a biohydrogen sensor after 72 hours. The result was the presence of hydrogen in all the 3 samples. After streaking, PCR and gel electrophoresis detected the bacteria which produced the hydrogen. RGCB Thiruvananthapuram conducted the sequencing of the PCR resultant. And identified the bacterial strains. Five variants of Bacillus bacteria ( (1) Bacillus cereus strain JTM GenBank: OP278839.1 (2) Bacillus toyonensis strain JTM2 GenBank: OP278841.1 (3) Bacillus anthracis strain JTM_SR2989-3-R_H08 GenBank: OP278960.1 (4) Bacillus thuringiensis strain JRY1 GenBank: OP278976.1 (5) Bacillus anthracis strain JTM_SR2989-3-F_H07 GenBank: OP278959.1 ) are identified and successfully registered in NCBI Gen bank. These Bacillus bacteria are major types of Rhizobacteria that can form spores and can survive in the soil for a long time period under harsh environmental conditions. Also, plant growth is enhanced by PGPR (Plant growth promoting rhizobacteria) through the induction of systemic resistance, antibiosis, and competitive omission. The molecular sequencing was submitted to the NCBI Gen Bank, and the accession numbers were allotted for the bacterial cultures. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bio%20hydrogen%20production" title="bio hydrogen production">bio hydrogen production</a>, <a href="https://publications.waset.org/abstracts/search?q=bacterial%20bio%20hydrogen%20production" title=" bacterial bio hydrogen production"> bacterial bio hydrogen production</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20related%20to%20bacillus%20bacteria." title=" plant related to bacillus bacteria."> plant related to bacillus bacteria.</a>, <a href="https://publications.waset.org/abstracts/search?q=bacillus%20bacteria%20study" title=" bacillus bacteria study"> bacillus bacteria study</a> </p> <a href="https://publications.waset.org/abstracts/178926/biohydrogen-production-from-rice-water-using-bacteria-isolated-from-wetland-sediment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/178926.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">66</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10445</span> Productivity and Profitability of Field Pea as Influenced by Different Levels of Fertility and Bio-Fertilizers under Irrigated Condition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Akhilesh%20Mishra">Akhilesh Mishra</a>, <a href="https://publications.waset.org/abstracts/search?q=Geeta%20Rai"> Geeta Rai</a>, <a href="https://publications.waset.org/abstracts/search?q=Arvind%20Srivastava"> Arvind Srivastava</a>, <a href="https://publications.waset.org/abstracts/search?q=Nalini%20Tiwari"> Nalini Tiwari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A field experiment was conducted during two consecutive Rabi seasons of 2007 and 2008 to study the economics of different bio-fertilizer’s inoculations in fieldpea (cv. Jai) at Chandra Shekhar Azad University of Agriculture and Technology, Kanpur (India). Results indicated that the seed inoculation with Rhizobium + PSB + PGPR improved all the growth; yield attributes and yields of field pea. Fresh and dry weight plant-1, nodules number and dry weight plant-1 were found significantly maximum. Number of grains pod-1, number and weight of pods plant-1 at maturity attributed significantly in increasing the grain yield as well as net return. On pooled basis, maximum net income (Rs.22169 ha-1) was obtained with the use of Rhizobium + PSB + PGPR which was improved by a margin of Rs.1502 (6.77%), 2972 (13.40%), 2672 (12.05%), 5212 (23.51%), 6176 (27.85%), 4666 (21.04%) and 8842/ha (39.88%) over the inoculation of PSB + PGPR, Rhizobium + PGPR, Rhizobium + PSB, PGPR, PSB, Rhizobium and control, respectively. Thus, it can be recommended that to earn the maximum net profit from dwarf field pea, seed should be inoculated with Rhizobium + PSB + PGPR. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rhizobium" title="rhizobium">rhizobium</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphorus%20solubilizing%20bacteria" title=" phosphorus solubilizing bacteria"> phosphorus solubilizing bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20promoting%20rhizobacteria" title=" plant growth promoting rhizobacteria"> plant growth promoting rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=field%20pea" title=" field pea"> field pea</a> </p> <a href="https://publications.waset.org/abstracts/10422/productivity-and-profitability-of-field-pea-as-influenced-by-different-levels-of-fertility-and-bio-fertilizers-under-irrigated-condition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10422.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">409</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10444</span> Nature-based Solutions for Mitigating the Impact of Climate Change on Plants: Utilizing Encapsulated Plant Growth Regulators and Associative Microorganisms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Raana%20Babadi%20Fathipour">Raana Babadi Fathipour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Over the past decades, the climatic CO2 concentration and worldwide normal temperature have been expanding, and this drift is anticipated to before long gotten to be more extreme. This situation of climate alter escalate abiotic stretch components (such as dry spell, flooding, saltiness, and bright radiation) that debilitate timberland and related environments as well as trim generation. These variables can contrarily influence plant development and advancement with a ensuing lessening in plant biomass aggregation and surrender, in expansion to expanding plant defenselessness to biotic stresses. As of late, biostimulants have ended up a hotspot as an viable and economical elective to reduce the negative impacts of stresses on plants. In any case, the larger part of biostimulants has destitute solidness beneath natural conditions, which leads to untimely debasement, shortening their organic movement. To unravel these bottlenecks, small scale- and nano-based definitions containing biostimulant atoms and/or microorganisms are picking up consideration as they illustrate a few points of interest over their routine details. In this survey, we center on the embodiment of plant development controllers and plant acquainted microorganisms as a technique to boost their application for plant assurance against abiotic stresses. We moreover address the potential restrictions and challenges confronted for the execution of this innovation, as well as conceivable outcomes with respect to future inquire about. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bio%20stimulants" title="bio stimulants">bio stimulants</a>, <a href="https://publications.waset.org/abstracts/search?q=Seed%20priming" title=" Seed priming"> Seed priming</a>, <a href="https://publications.waset.org/abstracts/search?q=nano%20biotechnology" title=" nano biotechnology"> nano biotechnology</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth-promoting" title=" plant growth-promoting"> plant growth-promoting</a>, <a href="https://publications.waset.org/abstracts/search?q=rhizobacteria" title=" rhizobacteria"> rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20regulators" title=" plant growth regulators"> plant growth regulators</a>, <a href="https://publications.waset.org/abstracts/search?q=microencapsulation" title=" microencapsulation"> microencapsulation</a> </p> <a href="https://publications.waset.org/abstracts/176781/nature-based-solutions-for-mitigating-the-impact-of-climate-change-on-plants-utilizing-encapsulated-plant-growth-regulators-and-associative-microorganisms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/176781.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">69</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10443</span> Plant Growth and Yield Enhancement of Soybean by Inoculation with Symbiotic and Nonsymbiotic Bacteria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Timea%20I.%20Hajnal-Jafari">Timea I. Hajnal-Jafari</a>, <a href="https://publications.waset.org/abstracts/search?q=Simonida%20S.%20%C4%90uri%C4%87"> Simonida S. Đurić</a>, <a href="https://publications.waset.org/abstracts/search?q=Dragana%20R.%20Stamenov"> Dragana R. Stamenov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microbial inoculants from the group of symbiotic-nitrogen-fixing rhizobia are well known and widely used in production of legumes. On the other hand, nonsymbiotic plant growth promoting rhizobacteria (PGPR) are not commonly used in practice. The objective of this study was to examine the effects of soybean inoculation with symbiotic and nonsymbiotic bacteria on plant growth and seed yield of soybean. Microbiological activity in rhizospheric soil was also determined. The experiment was set up using a randomized block system in filed conditions with the following treatments: control-no inoculation; treatment 1-Bradyrhizobium japonicum; treatment 2-Azotobacter sp.; treatment 3-Bacillus sp..In the flowering stage of growth (FS) the number of nodules per plant (NPP), root length (RL), plant height (PH) and weight (PW) were measured. The number of pod per plant (PPP), number of seeds per pod (SPP) and seed weight per plant (SWP) were recorded at the end of vegetation period (EV). Microbiological analyses of soil included the determination of total number of bacteria (TNB), number of fungi (FNG), actinomycetes (ACT) and azotobacters (AZB) as well as the activity of the dehydrogenase enzyme (DHA). The results showed that bacterial inoculation led to the formation of root nodules regardless of the treatments with statistically no significant difference. Strong nodulation was also present in control treatment. RL and PH were positively influenced by inoculation with Azotobacter sp. and Bacillus sp., respectively. Statistical analyses of the number of PPP, SPP, and SWP showed no significant differences among investigated treatments. High average number of microorganisms were determined in all treatments. Most abundant were TNB (log No 8,010) and ACT (log No 6,055) than FNG and AZB with log No 4,867 and log No 4,025, respectively. The highest DHA activity was measured in the FS of soybean in treatment 3. The application of nonsymbiotic bacteria in soybean production can alleviate initial plant growth and help the plant to better overcome different stress conditions caused by abiotic and biotic factors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteria" title="bacteria">bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=inoculation" title=" inoculation"> inoculation</a>, <a href="https://publications.waset.org/abstracts/search?q=soybean" title=" soybean"> soybean</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20activity" title=" microbial activity"> microbial activity</a> </p> <a href="https://publications.waset.org/abstracts/80297/plant-growth-and-yield-enhancement-of-soybean-by-inoculation-with-symbiotic-and-nonsymbiotic-bacteria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80297.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">152</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10442</span> Metabolic Regulation of Rhizobacteria for Cool-Season Grass Tolerance to Heat Stress</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kashif%20Jaeel">Kashif Jaeel</a>, <a href="https://publications.waset.org/abstracts/search?q=Bingru%20Huang"> Bingru Huang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Stress-induced accumulation of ethylene exacerbates drought damages in plants, and suppressing stress induction of ethylene may promote plant tolerance to heat stress. The objective of this study was to investigate the effects of endophytic bacteria (Paraburkholderia aspalathi) with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzymes in suppressing ethylene production on plant tolerance to heat stress and underlying physiological mechanisms of P. aspalathi-regulation in creeping bentgrass (Agrostis stolonifera). A novel strain of P. aspalathi, ‘WSF23’, with ACC deaminase activity was used to inoculate the roots of plants (cv. ‘Penncross’) subjected to heat stress in controlled-environment chambers. Inoculation with WSF23 bacteria resulted in improved shoot and root growth during heat stress. The differential changes in metabolite regulation due to the bacterial inoculation could contribute to ACC deamination bacteria-improved heat tolerance in cool-season grass species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rhizobacteria" title="rhizobacteria">rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=grass" title=" grass"> grass</a>, <a href="https://publications.waset.org/abstracts/search?q=heat" title=" heat"> heat</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20metabolism" title=" plant metabolism"> plant metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20bacteria" title=" soil bacteria"> soil bacteria</a> </p> <a href="https://publications.waset.org/abstracts/177551/metabolic-regulation-of-rhizobacteria-for-cool-season-grass-tolerance-to-heat-stress" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/177551.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">67</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10441</span> Diversity, Phyto Beneficial Activities and Agrobiotechnolody of Plant Growth Promoting Bacillus and Paenibacillus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cheba%20Ben%20Amar">Cheba Ben Amar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacillus and Paenibacillus are Gram-positive aerobic endospore-forming bacteria (AEFB) and most abundant in the rhizosphere, they mediated plant growth promotion and disease protection by several complex and interrelated processes involving direct and indirect mechanisms that include nitrogen fixation, phosphate solubilization, siderophores production, phytohormones production and plant diseases control. In addition to their multiple PGPR properties, high secretory capacity, spore forming ability and spore resistance to unfavorable conditions enabling their extended commercial applications for long shelf-life. Due to these unique advantages, Bacillus species were the most an ideal candidate for developing efficient PGPR products such as biopesticides, fungicides and fertilizers. This review list all studied and reported plant growth promoting Bacillus species and strains, discuss their capacities to enhance plant growth and protection with special focusing on the most frequent species Bacillus subtilis, B. pumilus ,B. megaterium, B. amyloliquefaciens , B. licheniformis and B. sphaericus, furthermore we recapitulate the beneﬁcial activities and mechanisms of several species and strains of the genus Paenibacillus involved in plant growth stimulation and plant disease control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacillus" title="bacillus">bacillus</a>, <a href="https://publications.waset.org/abstracts/search?q=paenibacillus" title=" paenibacillus"> paenibacillus</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR" title=" PGPR"> PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=bene%EF%AC%81cial%20activities" title=" beneﬁcial activities"> beneﬁcial activities</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanisms" title=" mechanisms"> mechanisms</a>, <a href="https://publications.waset.org/abstracts/search?q=growth%20promotion" title=" growth promotion"> growth promotion</a>, <a href="https://publications.waset.org/abstracts/search?q=disease%20control" title=" disease control"> disease control</a>, <a href="https://publications.waset.org/abstracts/search?q=agrobiotechnology" title=" agrobiotechnology"> agrobiotechnology</a> </p> <a href="https://publications.waset.org/abstracts/37958/diversity-phyto-beneficial-activities-and-agrobiotechnolody-of-plant-growth-promoting-bacillus-and-paenibacillus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37958.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">400</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10440</span> Effects of Plant Growth Promoting Microbes and Mycorrhizal Fungi on Wheat Growth in the Saline Soil</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Elgharably">Ahmed Elgharably</a>, <a href="https://publications.waset.org/abstracts/search?q=Nivien%20Nafady"> Nivien Nafady</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Arbuscular mycorrhizal fungi (AMF) and plant growth promoting microbes (PGPM) can promote plant growth under saline conditions. This study investigated how AMF and PGPM affected the growth and grain yield of wheat at different soil salinity levels (0, 75 and 150 mM NaCl). AMF colonization percentage, grain yield and dry weights and lengths of shoot and root, N, P K, Na, malondialdehyde, chlorophyll and proline contents and shoot relative permeability were determined. Salinity reduced NPK uptake and malondialdehyde and chlorophyll contents, and increased shoot Na concentration, relative permeability, and proline content, and thus declined plant growth. PGPM inoculation enhanced AMF colonization, P uptake, and K/Na ratio, but alone had no significant effect on plant growth and grain yield. AMF inoculation significantly enhanced NPK uptake, increased chlorophyll content and decreased shoot relative permeability, proline and Na contents, and thus promoted the plant growth. The inoculation of PGPM significantly enhanced the positive effects of AMF in controlling Na uptake and in increasing chlorophyll and NPK contents. Compared to AMF inoculation alone, dual inoculation with AMF and PGPM resulted in approximately 10, 25 and 25% higher grain yield at 0, 75 and 150 mM NaCl, respectively. The results provide that PGPM inoculation can maximize the effects of AMF inoculation in alleviating the deleterious effects of NaCl salts on wheat growth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mycorrhizal%20fungi" title="mycorrhizal fungi">mycorrhizal fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=salinity" title=" salinity"> salinity</a>, <a href="https://publications.waset.org/abstracts/search?q=sodium" title=" sodium"> sodium</a>, <a href="https://publications.waset.org/abstracts/search?q=wheat" title=" wheat"> wheat</a> </p> <a href="https://publications.waset.org/abstracts/102437/effects-of-plant-growth-promoting-microbes-and-mycorrhizal-fungi-on-wheat-growth-in-the-saline-soil" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/102437.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">180</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10439</span> The Use of Bituminaria bituminosa (L.) Stirton and Microbial Biotechnologies for Restoration of Degraded Pastoral Lands: The Case of the Middle Atlas of Morocco</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=O.%20Zennouhi">O. Zennouhi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20El%20Mderssa"> M. El Mderssa</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Ibijbijen"> J. Ibijbijen</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Bouiamrine"> E. Bouiamrine</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Nassiri"> L. Nassiri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rangelands and silvopastoral systems of the middle Atlas are under a heavy pressure, which led to pasture degradation, invasion by non-palatable and toxic species and edaphic aridification due to the regression of the global vegetation cover. In this situation, the introduction of multipurpose leguminous shrubs, such as <em>Bituminaria bituminosa</em> (L.) Stirton, commonly known as bituminous clover, could be a promising socio-ecological alternative for the rehabilitation of these degraded areas. The application of biofertilizers like plant growth promoting rhizobacteria especially phosphate solubilizing bacteria (PSB) can ensure a successful installation of this plant in the selected degraded areas. The main objective of the present work is to produce well-inoculated seedlings using the best efficient PSB strains in the greenhouse to increase their ability to resist to environmental constraints once transplanted to the field in the central Middle Atlas. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofertilizers" title="biofertilizers">biofertilizers</a>, <a href="https://publications.waset.org/abstracts/search?q=bituminaria%20bituminosa" title=" bituminaria bituminosa"> bituminaria bituminosa</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphate%20solubilizing%20bacteria" title=" phosphate solubilizing bacteria"> phosphate solubilizing bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=rehabilitation" title=" rehabilitation"> rehabilitation</a> </p> <a href="https://publications.waset.org/abstracts/97587/the-use-of-bituminaria-bituminosa-l-stirton-and-microbial-biotechnologies-for-restoration-of-degraded-pastoral-lands-the-case-of-the-middle-atlas-of-morocco" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/97587.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">150</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10438</span> Application of Free Living Nitrogen Fixing Bacteria to Increase Productivity of Potato in Field</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Govinda%20Pathak">Govinda Pathak</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In modern agriculture, the sustainable enhancement of crop productivity while minimizing environmental impacts remains a paramount challenge. Plant Growth Promoting Rhizobacteria (PGPR) have emerged as a promising solution to address this challenge. The rhizosphere, the dynamic interface between plant roots and soil, hosts intricate microbial interactions crucial for plant health and nutrient acquisition. PGPR, a subset of rhizospheric microorganisms, exhibit multifaceted beneficial effects on plants. Their abilities to stimulate growth, confer stress tolerance, enhance nutrient availability, and suppress pathogens make them invaluable contributors to sustainable agriculture. This work examines the pivotal role of free living nitrogen fixer in optimizing agricultural practices. We delve into the intricate mechanisms underlying PGPR-mediated plant-microbe interactions, encompassing quorum sensing, root exudate modulation, and signaling molecule exchange. Furthermore, we explore the diverse strategies employed by PGPR to enhance plant resilience against abiotic stresses such as drought, salinity, and metal toxicity. Additionally, we highlight the role of PGPR in augmenting nutrient acquisition and soil fertility through mechanisms such as nitrogen fixation, phosphorus solubilization, and mineral mobilization. Furthermore, we discuss the potential of PGPR in minimizing the reliance on chemical fertilizers and pesticides, thereby contributing to environmentally friendly agriculture. However, harnessing the full potential of PGPR requires a comprehensive understanding of their interactions with host plants and the surrounding microbial community. We also address challenges associated with PGPR application, including formulation, compatibility, and field efficacy. As the quest for sustainable agriculture intensifies, harnessing the remarkable attributes of PGPR offers a holistic approach to propel agricultural productivity while maintaining ecological balance. This work underscores the promising prospect of free living nitrogen fixer as a panacea for addressing critical agricultural challenges regarding chemical urea in an era of sustainable and resilient food production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=PGPR" title="PGPR">PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrogen%20fixer" title=" nitrogen fixer"> nitrogen fixer</a>, <a href="https://publications.waset.org/abstracts/search?q=quorum%20sensing" title=" quorum sensing"> quorum sensing</a>, <a href="https://publications.waset.org/abstracts/search?q=Rhizobacteria" title=" Rhizobacteria"> Rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides" title=" pesticides"> pesticides</a> </p> <a href="https://publications.waset.org/abstracts/182162/application-of-free-living-nitrogen-fixing-bacteria-to-increase-productivity-of-potato-in-field" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/182162.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">59</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10437</span> Potential Application of Selected Halotolerant PSB Isolated from Rhizospheric Soil of Chenopodium quinoa in Plant Growth Promotion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ismail%20Mahdi">Ismail Mahdi</a>, <a href="https://publications.waset.org/abstracts/search?q=Nidal%20Fahsi"> Nidal Fahsi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Hafidi"> Mohamed Hafidi</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdelmounaim%20Allaoui"> Abdelmounaim Allaoui</a>, <a href="https://publications.waset.org/abstracts/search?q=Latefa%20Biskri"> Latefa Biskri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> To meet the worldwide demand for food, smart management of arable lands is needed. This could be achieved through sustainable approaches such as the use of plant growth-promoting microorganisms including bacteria. Phosphate (P) solubilization is one of the major mechanisms of plant growth promotion by associated bacteria. In the present study, we isolated and screened 14 strains from the rhizosphere of Chenopodium quinoa wild grown in the experimental farm of UM6P and assessed their plant growth promoting properties. Next, they were identified by using 16S rRNA and Cpn60 genes sequencing as Bacillus, Pseudomonas and Enterobacter. These strains showed dispersed capacities to solubilize P (up to 346 mg L−1) following five days of incubation in NBRIP broth. We also assessed their abilities for indole acetic acid (IAA) production (up to 795,3 µg ml−1) and in vitro salt tolerance. Three Bacillus strains QA1, QA2, and S8 tolerated high salt stress induced by NaCl with a maximum tolerable concentration of 8%. Three performant isolates, QA1, S6 and QF11, were further selected for seed germination assay because of their pronounced abilities in terms of P solubilization, IAA production and salt tolerance. The early plant growth potential of tested strains showed that inoculated quinoa seeds displayed greater germination rate and higher seedlings growth under bacterial treatments. The positive effect on seed germination traits strongly suggests that the tested strains are growth promoting, halotolerant and P solubilizing bacteria which could be exploited as biofertilizers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phosphate%20solubilizing%20bacteria" title="phosphate solubilizing bacteria">phosphate solubilizing bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=IAA" title=" IAA"> IAA</a>, <a href="https://publications.waset.org/abstracts/search?q=Seed%20germination" title=" Seed germination"> Seed germination</a>, <a href="https://publications.waset.org/abstracts/search?q=salt%20tolerance" title=" salt tolerance"> salt tolerance</a>, <a href="https://publications.waset.org/abstracts/search?q=quinoa" title=" quinoa"> quinoa</a> </p> <a href="https://publications.waset.org/abstracts/125261/potential-application-of-selected-halotolerant-psb-isolated-from-rhizospheric-soil-of-chenopodium-quinoa-in-plant-growth-promotion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/125261.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">131</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10436</span> Selection of Lead Mobilizing Bacteria from Contaminated Soils and Their Potential in Promoting Plant Growth through Plant Growth Promoting Activity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maria%20Manzoor">Maria Manzoor</a>, <a href="https://publications.waset.org/abstracts/search?q=Iram%20Gul"> Iram Gul</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Arshad"> Muhammad Arshad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacterial strains were isolated from contaminated soil collected from Rawalpindi and Islamabad. The strains were investigated for lead resistance and their effect on Pb solubility and PGPR activity. Incubation experiments were carried for inoculated and unoculated soil containing different levels of Pb. Results revealed that few stains (BTM-4, BTM-11, BTM-14) were able to tolerate Pb up to 600 mg L-1, whereas five strains (BTM-3, BTM-6, BTM-10, BTM-21 and BTM-24) showed significant increase in solubility of Pb when compared to all other strains and control. The CaCl2 extractable Pb was increased by 13.6, 6.8, 4.4 and 2.4 folds compared to un-inoculated control soil at increased soil Pb concentration (500, 1000, 1500 and 200 mg kg-1, respectively). The selected bacterial strains (11) were further investigated for plant growth promotion activity through PGPR assays including. Germination and root elongation assays were also conducted under elevated metal concentration in controlled conditions to elucidate the effects of microbial strains upon plant growth and development. The results showed that all the strains tested in this study, produced significantly varying concentrations of IAA, siderophores and gibberellic acid along with ability to phosphorus solubilization index (PSI). The results of germination and root elongation assay further confirmed the beneficial role of the microbial strains in elevating metal stress through PGPR activity. Among all tested strains, BTM-10 significantly improved plant growth. 1.3 and 2.7 folds increase in root and shoot length was observed when compared to control. Which may be attributed to presence of important plant growth promoting enzymes (IAA 74.6 μg/ml; GA 19.23 μg/ml; Sidrophore units 49% and PSI 1.3 cm). The outcome of this study indicates that these Pb tolerant and solubilizing strains may have the potential for plant growth promotion under metal stress and can be used as mediator when coupled with heavy metal hyperaccumulator plants for phytoremediation of Pb contaminated soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pb%20resistant%20bacteria" title="Pb resistant bacteria">Pb resistant bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=Pb%20mobilizing%20bacteria" title=" Pb mobilizing bacteria"> Pb mobilizing bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=Phytoextraction%20of%20Pb" title=" Phytoextraction of Pb"> Phytoextraction of Pb</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR%20activity%20of%20bacteria" title=" PGPR activity of bacteria"> PGPR activity of bacteria</a> </p> <a href="https://publications.waset.org/abstracts/70496/selection-of-lead-mobilizing-bacteria-from-contaminated-soils-and-their-potential-in-promoting-plant-growth-through-plant-growth-promoting-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70496.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">219</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10435</span> Evaluation of the Role of Bacteria-Derived Flavins as Plant Growth Promoting Molecules</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nivethika%20Ajeethan">Nivethika Ajeethan</a>, <a href="https://publications.waset.org/abstracts/search?q=Lord%20Abbey"> Lord Abbey</a>, <a href="https://publications.waset.org/abstracts/search?q=Svetlana%20Yurge"> Svetlana Yurge</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Riboflavin is a water-soluble vitamin and the direct precursor of the flavin cofactors flavin mononucleotide and flavin adenine dinucleotide. Flavins (FLs) are bioactive molecules that have a beneficial effect on plant growth and development. Sinorhizobium meliloti strain 1021 is an α-proteobacterium that forms agronomically important N₂-fixing symbiosis with Medicago plants and secretes a considerable amount of FLs (FL⁺ strain). This strain was also implicated in plant growth promotion in its association with non-legume host plants. However, the mechanism of this plant growth promotion is not well understood. In this study, we evaluated the growth and development of tomato plants inoculated with S. meliloti 1021 and its mutant (FL⁻ strain) with limited ability to secrete FLs. Our preliminary experiments indicated that inoculation with FL⁺ strain significantly increased seedlings' root and shoot length and surface area compared to those of plants inoculated with FL⁻ strain. For example, the root lengths of 9-day old seedlings inoculated with FL⁺ strain were 35% longer than seedlings inoculated with the mutant. Proteomic approaches combined with the analysis of plant physiological responses such as growth and photosynthetic rate, stomatal conductance, transpiration rate, and chlorophyll content will be used to evaluate the host-plant response to bacteria-derived FLs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=flavin" title="flavin">flavin</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20promotion" title=" plant growth promotion"> plant growth promotion</a>, <a href="https://publications.waset.org/abstracts/search?q=riboflavin" title=" riboflavin"> riboflavin</a>, <a href="https://publications.waset.org/abstracts/search?q=Sinorhizobium%20meliloti" title=" Sinorhizobium meliloti"> Sinorhizobium meliloti</a> </p> <a href="https://publications.waset.org/abstracts/135412/evaluation-of-the-role-of-bacteria-derived-flavins-as-plant-growth-promoting-molecules" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/135412.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">154</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10434</span> Potential of Lead Tolerant and Mobilizing Fungus for Plant Growth Promotion through Plant Growth Promoting Activity; A Promising Approach for Enhance Phytoremediation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maria%20Manzoor">Maria Manzoor</a>, <a href="https://publications.waset.org/abstracts/search?q=Iram%20Gul"> Iram Gul</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Arshad"> Muhammad Arshad</a>, <a href="https://publications.waset.org/abstracts/search?q=Jean%20Kallerhoff"> Jean Kallerhoff</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The potential of fungal isolates to be used in phytoremediation of widespread lead contaminated soil has been evaluated in this study. Five different fungal isolates (Trichoderma harzianum, Penicillium simplicissimum, Aspergillus flavus, Aspergillus niger and Mucor spp.) were obtained and tested for their tolerance to increasing concentration of lead (Pb) i.e. 100, 200, 300, 400 and 500 mgL-1 on PDA and PDB culture experiment. All strains were tolerant up to 500 mgL-1 following sequence; A. flavus > A. niger > Mucor spp. > P. simplicissimum > T. harzianum. Further the isolates were then monitored for possible effect on Pb solubility/mobility through soil incubation experiments and characterized for essays including pathogenicity, germination and root elongation and plant growth promoting activities including IAA (indole acetic acid), phosphorus solubilization and gibberellic acid (GA3) production. Results revealed that fungal isolates have positive effect on Pb mobility in soil and plant biomass production. Pb solubility was significantly (P> 0.05) increased in soil upon application of Mucor spp. P. simplicissimum and T. harzianum. when compared to control. Among different strains three isolates (Mucor spp., P. simplicissimum and T. harzianum) were nonpathogenic because no inhibitory effect of fungus was observed to plant growth when exposed to these strains in root shoot elongation essay. Particularly T. harzianum and P. simplicissimum showed great ability to increase root length by 1.1 and 1.3 folds and shoot length by 1.47 and 1.5 folds respectively under Pb stress (500 mgL-1). Significantly high production of IAA was observed in A. niger (26.7 μg/ml), Phosphorus solubilization was observed in T. harzianum (9.15 μg/ml) and GA3 production was observed in P. simplicissimum (11.02 μg/ml). From results it is concluded that Mucor spp., P. simplicissimum and T. harzianum have potential to increase Pb mobility and improving plant growth under highy Pb contamination, therefore can be used in microbially assisted phytoremediation of Pb contaminated soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pb%20tolerant%20fungus" title="Pb tolerant fungus">Pb tolerant fungus</a>, <a href="https://publications.waset.org/abstracts/search?q=Pb%20mobility" title=" Pb mobility"> Pb mobility</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20promoting%20activities" title=" plant growth promoting activities"> plant growth promoting activities</a>, <a href="https://publications.waset.org/abstracts/search?q=indole%20acetic%20acid%20%28IAA%29" title=" indole acetic acid (IAA)"> indole acetic acid (IAA)</a> </p> <a href="https://publications.waset.org/abstracts/70524/potential-of-lead-tolerant-and-mobilizing-fungus-for-plant-growth-promotion-through-plant-growth-promoting-activity-a-promising-approach-for-enhance-phytoremediation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70524.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">269</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10433</span> Effects of Plant Growth Promoting Rhizobacteria on the Yield and Nutritive Quality of Tomato Fruits</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Narjes%20Dashti">Narjes Dashti</a>, <a href="https://publications.waset.org/abstracts/search?q=Nida%20Ali"> Nida Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Magdy%20Montasser"> Magdy Montasser</a>, <a href="https://publications.waset.org/abstracts/search?q=Vineetha%20Cherian"> Vineetha Cherian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The influence of two PGPR strains, Pseudomonas aeruginosa and Stenotrophomonas rhizophilia, on fruit yields, pomological traits and chemical contents of tomato (Solanum lycopersicum) fruits were studied. The study was conducted separately on two different cultivar varieties of tomato, namely Supermarmande and UC82B. The results indicated that the presence of the PGPR almost doubled the average yield per plant. There was a significant improvement in the pomological qualities of the PGPR treated tomato fruits compared to the corresponding healthy treatments especially in traits such as the average fruit weight, height, and fruit volume. The chemical analysis of tomato fruits revealed that the presence of the PGPRs increased the total protein, lycopene, alkalinity and phenol content of the tomato fruits compared to the healthy controls. They had no influence on the reduced sugar, total soluble solids or the titerable acid content of fruits. However their presence reduced the amount of ascorbic acid in tomato fruits compared to the healthy controls. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=PGPR" title="PGPR">PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=tomato" title=" tomato"> tomato</a>, <a href="https://publications.waset.org/abstracts/search?q=fruit%20quality" title=" fruit quality"> fruit quality</a> </p> <a href="https://publications.waset.org/abstracts/29728/effects-of-plant-growth-promoting-rhizobacteria-on-the-yield-and-nutritive-quality-of-tomato-fruits" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29728.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">327</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10432</span> Quality and Yield of Potato Seed Tubers as Influenced by Plant Growth Promoting Rhizobacteria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Raqib%20Rasul">Muhammad Raqib Rasul</a>, <a href="https://publications.waset.org/abstracts/search?q=Tavga%20Sulaiman%20Rashid"> Tavga Sulaiman Rashid</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fertilization increases efficiency and obtains better quality of product recovery in agricultural activities. However, fertilizer consumption increased exponentially throughout the world, causing severe environmental problems. Biofertilizers can be a practical approach to minimize chemical fertilizer sources and ultimately develop soil fertility. This study was carried out to isolate, identify and characterize bacteria from medicinal plant (Rumex tuberosus L. and Verbascum sp.) rhizosphere for in vivo screening. 25 bacterial isolates were isolated and several biochemical tests were performed. Two isolates that were positive for most biochemical tests were chosen for the field experiment. The isolates were identified as Go1 Alcaligenes faecalis (Accession No. OP001725) and T11 (Bacillus sp.) based on the 16S rRNA sequence analysis that was compared with related bacteria in GenBank database using MEGA 6.1. For the field trial isolate GO1 and T11 (separately and mixed), NPK as a positive control was used. Both isolates increased plant height, chlorophyll content, number of tubers, and tuber’s weight. The results demonstrated that these two isolates of bacteria can potentially replace with chemical fertilizers for potato production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofertilizer" title="biofertilizer">biofertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=Bacillus%20subtilis" title=" Bacillus subtilis"> Bacillus subtilis</a>, <a href="https://publications.waset.org/abstracts/search?q=Alcaligenes%20faecalis" title=" Alcaligenes faecalis"> Alcaligenes faecalis</a>, <a href="https://publications.waset.org/abstracts/search?q=potato%20tubers" title=" potato tubers"> potato tubers</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo%20screening" title=" in vivo screening"> in vivo screening</a> </p> <a href="https://publications.waset.org/abstracts/155470/quality-and-yield-of-potato-seed-tubers-as-influenced-by-plant-growth-promoting-rhizobacteria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/155470.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">103</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10431</span> Sustainable Agriculture Practices Using Bacterial-mediated Alleviation of Salinity Stress in Crop Plants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Trigui">Mohamed Trigui</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatma%20Masmoudi"> Fatma Masmoudi</a>, <a href="https://publications.waset.org/abstracts/search?q=Imen%20Zouari"> Imen Zouari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Massive utilizations of chemical fertilizer and chemical pesticides in agriculture sector to improve the farming productivity have created increasing environmental damages. Then, agriculture must become sustainable, focusing on production systems that respect the environment and help to reduce climate change. Isolation and microbial identification of new bacterial strains from naturally saline habitats and compost extracts could be a prominent way in pest management and crop production under saline conditions. In this study, potential mechanisms involved in plant growth promotion and suppressive activity against fungal diseases of a compost extract produced from poultry manure/olive husk compost and halotolerant and halophilic bacterial strains under saline stress were investigated. On the basis of the antimicrobial tests, different strains isolated from Sfax solar saltern (Tunisia) and from compost extracts were selected and tested for their plant growth promoting traits, such as siderophores production, nitrogen fixation, phosphate solubilization and the production of extracellular hydrolytic enzymes (protease and lipase) under in-vitro conditions. Among 450 isolated bacterial strains, 16 isolates showed potent antifungal activity against the tested plant pathogenic fungi. Their identification based on 16S rRNA gene sequence revealed they belonged to different species. Some of these strains were also characterized for their plant growth promoting capacities. Obtained results showed the ability of four strains belonging to Bacillus genesis to ameliorate germination rate and root elongation compared to the untreated positive controls. Combinatorial capacity of halotolerant bacteria with antimicrobial activity and plant growth promoting traits could be promising sources of interesting bioactive substances under saline stress. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=abiotic%20stress" title="abiotic stress">abiotic stress</a>, <a href="https://publications.waset.org/abstracts/search?q=biofertilizer" title=" biofertilizer"> biofertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=biotic%20stress" title=" biotic stress"> biotic stress</a>, <a href="https://publications.waset.org/abstracts/search?q=compost%20extract" title=" compost extract"> compost extract</a>, <a href="https://publications.waset.org/abstracts/search?q=halobacteria" title=" halobacteria"> halobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20growth%20promoting%20%28PGP%29" title=" plant growth promoting (PGP)"> plant growth promoting (PGP)</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20fertility" title=" soil fertility"> soil fertility</a> </p> <a href="https://publications.waset.org/abstracts/166274/sustainable-agriculture-practices-using-bacterial-mediated-alleviation-of-salinity-stress-in-crop-plants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/166274.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">91</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10430</span> Biofertilization of Cucumber (Cucumis sativus L.) Using Trichoderma longibrachiatum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kehinde%20T.%20Kareem">Kehinde T. Kareem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The need to increase the production of cucumber has led to the use of inorganic fertilizers. This chemical affects the ecological balance of nature by increasing the nitrogen and phosphorus contents of the soil. Surface runoffs into rivers and streams cause eutrophication which affects aquatic organisms as well as the consumers of aquatic animals. Therefore, this study was carried out in the screenhouse to investigate the use of a plant growth-promoting fungus; Trichoderma longibrachiatum for the growth promotion of conventional and in-vitro propagated Ashley and Marketmoor cucumber. Before planting of cucumber, spore suspension (108 cfu/ml) of Trichoderma longibrachiatum grown on Potato dextrose agar (PDA) was inoculated into the soil. Fruits were evaluated for the presence of Trichoderma longibrachiatum using a species-specific primer. Results revealed that the highest significant plant height produced by in-vitro propagated Ashley was 19 cm while the highest plant height of in-vitro propagated Marketmoor was 19.67 cm. The yield of the conventional propagated Ashley cucumber showed that the number of fruit/plant obtained from T. longibrachiatum-fertilized plants were significantly more than those of the control. The in-vitro Ashely had 7 fruits/plant while the control produced 4 fruits/plant. In-vitro Marketmoor had ten fruits/plant, and the control had a value of 4 fruits/plant. There were no traces of Trichoderma longibrachiatum genes in the harvested cucumber fruits. Therefore, the use of Trichoderma longibrachiatum as a plant growth-promoter is safe for human health as well as the environment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofertilizer" title="biofertilizer">biofertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=cucumber" title=" cucumber"> cucumber</a>, <a href="https://publications.waset.org/abstracts/search?q=genes" title=" genes"> genes</a>, <a href="https://publications.waset.org/abstracts/search?q=growth-promoter" title=" growth-promoter"> growth-promoter</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro" title=" in-vitro"> in-vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=propagation" title=" propagation"> propagation</a> </p> <a href="https://publications.waset.org/abstracts/56965/biofertilization-of-cucumber-cucumis-sativus-l-using-trichoderma-longibrachiatum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56965.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">244</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10429</span> Impact of Elevated Temperature on Spot Blotch Development in Wheat and Induction of Resistance by Plant Growth Promoting Rhizobacteria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jayanwita%20Sarkar">Jayanwita Sarkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Usha%20Chakraborty"> Usha Chakraborty</a>, <a href="https://publications.waset.org/abstracts/search?q=Bishwanath%20Chakraborty"> Bishwanath Chakraborty</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plants are constantly interacting with various abiotic and biotic stresses. In changing climate scenario plants are continuously modifying physiological processes to adapt to changing environmental conditions which profoundly affect plant-pathogen interactions. Spot blotch in wheat is a fast-rising disease in the warmer plains of South Asia where the rise in minimum average temperature over most of the year already affecting wheat production. Hence, the study was undertaken to explore the role of elevated temperature in spot blotch disease development and modulation of antioxidative responses by plant growth promoting rhizobacteria (PGPR) for biocontrol of spot blotch at high temperature. Elevated temperature significantly increases the susceptibility of wheat plants to spot blotch causing pathogen Bipolaris sorokiniana. Two PGPR Bacillus safensis (W10) and Ochrobactrum pseudogrignonense (IP8) isolated from wheat (Triticum aestivum L.) and blady grass (Imperata cylindrical L.) rhizophere respectively, showing in vitro antagonistic activity against Bipolaris sorokiniana were tested for growth promotion and induction of resistance against spot blotch in wheat. GC-MS analysis showed that Bacillus safensis (W10) and Ochrobactrum pseudogrignonense (IP8) produced antifungal and antimicrobial compounds in culture. Seed priming with these two bacteria significantly increase growth, modulate antioxidative signaling and induce resistance and eventually reduce disease incidence in wheat plants at optimum as well as elevated temperature which was further confirmed by indirect immunofluorescence assay using polyclonal antibody raised against Bipolaris sorokiniana. Application of the PGPR led to enhancement in activities of plant defense enzymes- phenylalanine ammonia lyase, peroxidase, chitinase and β-1,3 glucanase in infected leaves. Immunolocalization of chitinase and β-1,3 glucanase in PGPR primed and pathogen inoculated leaf tissue was further confirmed by transmission electron microscopy using PAb of chitinase, β-1,3 glucanase and gold labelled conjugates. Activity of ascorbate-glutathione redox cycle related enzymes such as ascorbate peroxidase, superoxide dismutase and glutathione reductase along with antioxidants such as carotenoids, glutathione and ascorbate and osmolytes like proline and glycine betain accumulation were also increased during disease development in PGPR primed plant in comparison to unprimed plants at high temperature. Real-time PCR analysis revealed enhanced expression of defense genes- chalcone synthase and phenyl alanineammonia lyase. Over expression of heat shock proteins like HSP 70, small HSP 26.3 and heat shock factor HsfA3 in PGPR primed plants effectively protect plants against spot blotch infection at elevated temperature as compared with control plants. Our results revealed dynamic biochemical cross talk between elevated temperature and spot blotch disease development and furthermore highlight PGPR mediated array of antioxidative and molecular alterations responsible for induction of resistance against spot blotch disease at elevated temperature which seems to be associated with up-regulation of defense genes, heat shock proteins and heat shock factors, less ROS production, membrane damage, increased expression of redox enzymes and accumulation of osmolytes and antioxidants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidative%20enzymes" title="antioxidative enzymes">antioxidative enzymes</a>, <a href="https://publications.waset.org/abstracts/search?q=defense%20enzymes" title=" defense enzymes"> defense enzymes</a>, <a href="https://publications.waset.org/abstracts/search?q=elevated%20temperature" title=" elevated temperature"> elevated temperature</a>, <a href="https://publications.waset.org/abstracts/search?q=heat%20shock%20proteins" title=" heat shock proteins"> heat shock proteins</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR" title=" PGPR"> PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=Real-Time%20PCR" title=" Real-Time PCR"> Real-Time PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=spot%20blotch" title=" spot blotch"> spot blotch</a>, <a href="https://publications.waset.org/abstracts/search?q=wheat" title=" wheat"> wheat</a> </p> <a href="https://publications.waset.org/abstracts/85977/impact-of-elevated-temperature-on-spot-blotch-development-in-wheat-and-induction-of-resistance-by-plant-growth-promoting-rhizobacteria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/85977.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge 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