Sequencing Infrastructure

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itemprop="name">Infrastructure</span></a><meta itemprop="position" content="6"></li></ol></nav> <h1 class="mt-xs">Infrastructure</h1> </div> <nav aria-label="Men煤 terciari"> <ul role="list" class="sub-sub-nav is-flex is-flex-wrap-wrap has-text-weight-normal is-size-8 link-dn"> <li > <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/" class="pr-s">Introduction</a> </li> <li > <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/services-and-pricing/" class="pr-s">Services and pricing</a> </li> <li > <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/projects/" class="pr-s">Projects</a> </li> <li > <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/results/" class="pr-s">Results</a> </li> <li > <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/useful-information/" class="pr-s">Useful information</a> </li> <li class="active"> <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/infrastructure/" class="pr-s">Infrastructure</a> </li> <li > <a href="/en/serveis/core-facilities/technical-scientific-services/massive-sequencing-and-bioinformatics/contact/" class="pr-s">Contact</a> </li> </ul> </nav> <div class="text-content flow flow--3"> <h4><strong>Second generation</strong></h4> <ul> <li><strong>Illumina MiSeq</strong>: maximum sequence length capacity 2x300bp, total output up to 15GigaBase. Ideal for short amplicons or re-sequencing analyses.</li> <li><strong>Illumina NextSeq500</strong>: maximum sequence length capacity 2脳150, total output up to 120GigaBase. Ideal for short amplicons or re-sequencing analyses.</li> <li><strong>Illumina NextSeq2000 (<em>new</em>)</strong>: maximum sequence length capacity 2脳300, total output up to 360GigaBase. Ideal for (meta)genomic and (meta)transcriptomic analyses, short amplicons or for re-sequencing analyses.</li> </ul> <h4><img alt="" src="/media/upload/cache/img_3759_webp.webp" width="1500" height="937" loading="lazy"></h4> <h4><strong>Third generation</strong></h4> <ul> <li><strong>Oxford Nanopore MinIon</strong>: maximum capacity for long sequences (up to 100Kb), total output up to 30GigaBase. Ideal for genomic/metagenomic analyses where portability or speed in obtaining results is an important factor.</li> <li><strong>PacBio Sequel II (<em>new</em>)</strong>: capacity for ~4,000,000 HiFi sequences with a length of ~10Kb (Circular Consensus Sequences, CCS), and with a quality of ~Q80. Ideal for complete genomes in search of resistance factors, virulence or new genomic characteristics or fo long amplicons sequencing such as the microbial 16S ribosomal gene, reaching a depth of taxonomic annotation down to the <strong>species</strong> or <strong>strain</strong> level.</li> </ul> </div> </div> <div class="column is-one-third-tablet is-one-quarter-desktop"> <nav aria-label="Men煤 secundari"> <ul class="sub-nav link-dn has-text-weight-normal" role="list"> <li class=" "><a href="/es/apoyo-a-la-investigacion/plataformas-y-servicios-cientifico-tecnicos/biobancos/" class="py-xs is-block">Biobanks</a> <ul class="is-size-9 ml-xs mb-xs flow flow--1" aria-label="submenu" role="list"> <li><a href="/es/apoyo-a-la-investigacion/plataformas-y-servicios-cientifico-tecnicos/biobancos/red-valenciana-de-biobancos/" class="px-xs py-2xs">Red Valenciana de Biobancos</a> <ul class="has-text-weight-normal px-xs py-2xs" aria-label="submenu" role="list"> <li><a 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