GENEWIZ from Azenta | PCR Purification

<!DOCTYPE html>    <html lang="en">  <head> <title>GENEWIZ from Azenta | PCR Purification</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="GENEWIZ's PCR Purification service eliminates the need for costly and time-consuming column or gel-extraction clean ups."> <meta name="keywords" content="genewiz, azenta life sciences, un-purified pcr, pcr purification, pcr purification service, excess primers, unincorporated dntps, pcr sequencing templates, enzymatic purification process, enzymatic pcr clean-up, pcr, oligonucleotides, universal primers, column clean up, gel-extraction clean up, template, sanger sequencing, sanger, sanger dna sequencing"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/PCR-Purification/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/PCR-Purification" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/PCR-Purification" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/PCR-Purification" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Sanger-Sequencing/PCR-Purification" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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.sanger-content-box img { width: 400px; } .sanger-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .sanger-new-container { text-align: center; margin-top: 0; } .sanger-new-content { width: 90%; margin: 0 auto; } .sanger-new-content { display: inline-block; } #sanger-content-box-right, #sanger-content-box-left { width: 100%; text-align: center; } #sanger-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .sanger-new-content { width: 90%; margin: 0 auto; } .sanger-content-box img { width: 350px; } } </style> <div class="sanger-new-container"> <div class="sanger-new-content"> <div class="sanger-content-box" id="sanger-content-box-left"> <br /> <h1 style="text-transform: uppercase;">PCR Purification</h1> <div> </div> <p>Azenta Life Sciences, formerly GENEWIZ offers the added convenience of our PCR Purification service to eliminate the need for costly and time-consuming column or gel-extraction clean ups. Our enzymatic PCR clean-up technique removes excess primers and unincorporated dNTPs from PCR sequencing templates prior to the cycle sequencing reaction. Let Azenta remove the hassle from PCR clean-up and expedite your PCR product sequencing workflow.</p> <br /> <a rel="noopener noreferrer" href="https://clims4.genewiz.com/SangerSequencing?lobId=1&serviceId=103" target="_blank"><button>Place an Order</button></a> </div> <div class="sanger-content-box" id="sanger-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02mj2mK0" target="_blank"><img alt="" src="/-/media/Sanger-50-Off-First-Order.ashx?la=en&hash=81E1D55079527BF3CEF0E873B371A7E2C8FC05E3" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="process-v1 Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="row"> <h2>PCR Clean Up</h2> <p>Azenta uses a combination of enzymes to remove excess dNTPs and oligonucleotides from the PCR reaction prior to sequencing.  This approach generates a good-quality template that is ready to sequence, while avoiding common contamination problems from other, column-based purification techniques.</p> </div> <div class="row"> <h3>Enzymatic Purification Process</h3> <p style="width: 80%; margin: 0px auto; text-align: center;"><img alt="Enzymatic Purification Process" src="/-/media/Images/INFO_Enzyme-Purification-Process.ashx?la=en&hash=3EE3AAB266F33CCB21CFE8DF448D8CA77B86AFC5" height="170" width="648" /></p> </div> <div class="row margin-top-30"> <h2>Technical Notes</h2> <div class="col-sm-6 text-left"> <img src="~/media/Images/Services/GA/photos/technical-notes_375x561.ashx?h=220&la=en&w=330&hash=4F519E3A104D640AD2D58EC0B768CA223C007741" alt="Building" class="img-responsive center-block" style="height: 220px; width: 330px;" /> </div> <div class="col-sm-6 text-left"> <p><span id="fld_F38A95B5D92A43F7B84CEEC496A15286_3BC76BA4EE5C433DB9FDA754AA84AC64_en_1_edeb421550c749e380584fb89a6f81bb_8927_edit" class="scWebEditInput" sc-part-of="field" contenteditable="false"> In order to optimize the concentration of the sequencing reaction, GENEWIZ recommends submitting a representative gel image of your PCR products along with your samples and order receipt. <br /> </span></p> <p>This approach purifies the entire PCR reaction, and can only be used for single-band PCR products. Occasionally, certain brands of high-fidelity <em>Taq </em>can cause interference with the enzymatic cleanup reaction. Please contact <a href="mailto:DNAseq@genewiz.com">Technical Support</a> if you would like to discuss your specific enzyme selection.</p> </div> </div> </div> </div> <br /> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11038-D_PEZ-DirectColonySeq_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 15px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner1 { width: 100%; height: auto; } .covid-content1 { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <br /> <h3><span style="color: #55c8d2;">NEW</span> Direct Colony to<br /> Whole Plasmid Sequencing</h3> <a href="https://hubs.ly/Q02FKw360" target="_blank"> <br /> <button> LEARN MORE</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>FEATURES & BENEFITS</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Time savings and cost savings - no need for time-consuming column purifications<br> <br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Analysis using ABI Prism 3730xl DNA analyzers </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Free Azenta <a href="/en/Public/Resources/Free-Universal-Primers">Universal Primers</a> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Customized troubleshooting </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Fast turnaround </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> >500 bases of Phred20 reads </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Azenta Online Ordering and Data Retrieval System - 24/7 web-based </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> Project design and consultation </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #ffffff; padding: 0; margin: 0;margin-top: -20px;"> <h2 style="border-bottom: 1px solid #f1f0f0;">TECHNICAL RESOURCES</h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Analyzing-Sanger-Blog.ashx?h=160&w=220&la=en&hash=8A9D75A95F2026E45E36D46F1028677640443783" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Analyzing Sanger Sequencing Data</h3> <p>The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data. <br /> <a href="https://www.azenta.com/learning-center/blog/analyzing-sanger-sequencing-data" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Webinar--Sanger-ONT.ashx?h=160&w=220&la=en&hash=B46A4AA56DE351DD45E75213BD6FD1AA5AB8F799" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing</h3> <p>While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs. <br /> <a href="https://web.genewiz.com/workshop/base-pairs-to-breakthroughs" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/qPCR-dPCR-Blog.ashx?h=160&w=220&la=en&hash=E76BF29417DDCA4C25770741C98732CFC291673F" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | NGS, qPCR, dPCR, or Sanger Sequencing: An Assay Selection Guide</h3> <p>Today’s molecular biologists have many options to interrogate their genome or transcriptome of interest. Next generation sequencing (NGS), quantitative PCR (qPCR), digital PCR (dPCR), and Sanger sequencing are used widely in genomics; however, choosing the best tool for your project isn’t always intuitive. To learn more about the method best suited for your project goals, use our interactive selection guide as well as the practical information about PCR + Sanger, qPCR, dPCR, and NGS approaches below. <br /> <a href="https://www.azenta.com/learning-center/blog/ngs-qpcr-or-sanger-sequencing-assay-selection-guide" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Trouble-shooting-blog.ashx?h=160&w=220&la=en&hash=24FECB44F887ED8D2D363153D5842A55828C120E" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Troubleshooting DNA Templates with Sanger Sequencing</h3> <p>Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.<br /> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <p> </p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <h3>From Base Pairs to Breakthroughs: Understanding<br /> Sanger and Oxford Nanopore Sequencing</h3> <p>Monday, September 16, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02Jnl9m0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div>  <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> <p>*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.</p> <br> </div> </div> <ul class="list-inline row process-in process-border-4 "> <li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a href='https://clims4.genewiz.com/RegisterAccount/Index ' target='_blank'> <div class="process-desc"> <img src="/-/media/Icon_90x90_CreateAccount.ashx?h=91&la=en&w=91&hash=5CEBCC7F68DB7C97789060FEA47816B7D3D3E6F7" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Create An Account</h3> </div> </a> </li><li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a href='/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines' target='_blank'> 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