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In-Cell ELISA Assays to Assess Drug Relative Potency Using the Cytation Imaging Reader for Sample Analysis | BioAgilytix

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/* color: #FFFFFF;*/ font-weight: 400; font-size: 16px; line-height: 26px; } /* Hide the original checkbox */ ul.inputs-list.multi-container li.hs-form-checkbox label input, .hs-form-booleancheckbox-display input { height: 24px; left: 0; opacity: 0; position: absolute; top: 0; width: 24px; } /* Add new checkbox */ ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hs-form-booleancheckbox-display span::before { content: ""; border-radius: 0px; background-color: #ffffff; position: absolute; top: 4px; left: 8px; z-index: 1; display: block; width: 5px; height: 10px; border: solid #157479; border-width: 0 2px 2px 0; -webkit-transform: rotate(45deg); -ms-transform: rotate(45deg); transform: rotate(45deg); opacity: 0; -webkit-transition-duration: .1s; -o-transition-duration: .1s; transition-duration: .1s; -webkit-transition-property: border-color; -o-transition-property: border-color; transition-property: border-color; } /* Style new checked item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ padding-right: 10px; } .hbspt-form .hs-lastname, .kits_form_col .hs_confirm_email{ padding-left: 10px; } .kits_form_col {padding-bottom: 108px;} .hs-richtext h4 {margin-bottom: 30px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 20px;} } @media screen and (max-width: 640px){ .hbspt-form h1{font-size: 32px !important;} .hbspt-form .gform_heading p{font-size: 16px !important;} .hbspt-form .form-columns-2 .field{ -webkit-box-flex: 0; -ms-flex: 0 0 100%; flex: 0 0 100%; margin-bottom: .5rem; padding-left: 0; } } @media screen and (max-width: 540px){ .event-hero-section > div{ justify-items: flex-start !important; } } @media screen and (max-width: 414px){ body .hubspot_signup_form .hs_email {width: 66% !important;} body .hubspot_signup_form ul.no-list{top: 65px !important;} } @media screen and (max-width: 320px){ body .hubspot_signup_form .hs_email {width: 61% !important;} }</style> <style type="text/css">.about-presenter-section{display:none;} .space-hero-section{margin-bottom:0px !important;} .partner_bioAgilytix {padding-right: 2.5rem !important;} @media screen and (max-width: 540px){ .event-hero-section > div{ justify-items: flex-start !important; } }</style> <style type="text/css">/* Add your CSS code here. 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These potency assays must be tailored to the drug mechanism of action (MOA) and provide a specific readout related to the drug鈥檚 biological properties. Many formats exist for measuring drug potency (i.e. ELISA, flow cytometry, western blot, etc.) and should be chosen based on the platform that can successfully show accurate potency of a particular drug candidate. Here we show the utility of the In-Cell ELISA assay platform to assess relative potency of multiple drug therapies using the Cytation10 and Cytation3 cell imaging readers. The Cytation reader is a useful tool for relative potency measurements because it allows for fully automated microscopy (brightfield, fluorescent and confocal) in a multi-well plate reader format that standard plate readers cannot provide. In combination with Gen5 software versions such as Image+ and Image Prime, the user can efficiently analyze data like a standard plate reader with the added imaging benefit that is necessary for an In-Cell ELISA. Both readers can produce image-based potency readouts but vary in their analytical functionality and data analysis settings based on software versions within Gen5. Image and data treatment are important components in the implementation of these methods and the advantages, utility, and considerations of these are discussed here using two case studies. One involves protein expression as a function of cell number while the second uses the reversal of protein aggregate formation as functional end points.</p><div class="wp-block-emagine-button gated-content__download-button em-mt--md em-mb--md" data-resource-type="file"> <a class="btn btn--size-md btn--style-primary has-primary-background-color" href="https://www.bioagilytix.com/wp-content/uploads/2024/06/2023_AAPS_PharmSci360_Poster_LAL.pdf" target="_blank" rel="noopener norefferer"> <span>Download Poster</span> </a></div><div class="pagination"><div class="nav-previous alignleft"><a href="https://www.bioagilytix.com/scientific-expertise/scientific-insights/points-to-consider-in-the-development-of-activity-and-potency-assays-for-enzyme-biotherapeutics/" rel="prev">Points to Consider in the Development of Activity and Potency Assays for Enzyme Biotherapeutics</a></div><div class="nav-next alignright"><a href="https://www.bioagilytix.com/scientific-expertise/scientific-insights/proof-of-concept-of-a-qualitative-qpcr-assay-for-rcl-monitoring/" rel="next">Proof-of-Concept of a Qualitative qPCR Assay for RCL Monitoring</a></div></div></div><div class="em-news--image-container"><div class="em-news__media"><div class="em-news__media-inner"> <img src="https://www.bioagilytix.com/wp-content/uploads/2024/06/in-cell_elsa_thumb.jpg" alt="" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2024/06/in-cell_elsa_thumb.jpg 800w, https://www.bioagilytix.com/wp-content/uploads/2024/06/in-cell_elsa_thumb-300x300.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2024/06/in-cell_elsa_thumb-150x150.jpg 150w, https://www.bioagilytix.com/wp-content/uploads/2024/06/in-cell_elsa_thumb-768x768.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2024/06/in-cell_elsa_thumb-600x600.jpg 600w, 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