EphB2 Antibody [Unconjugated] (AF467): Novus Biologicals

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Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-4120241049443.jpg","caption":"Expression of EphB2 increases and is activated on HSCs after chronic CCl4 -induced liver injury. (a) Isolated cell fractions from livers of mice subjected to chronic CCl4 injections were analyzed for EphB2, Ephrin-B1, Ephrin-B2 and Ephrin-B3 mRNA levels using RT-qPCR. Results are shown as fold change compared to liver cell fractions obtained from vehicle-treated controls. Error bars represent mean\u2009\u00b1\u2009SEM.; n\u2009=\u20096 animals; CD11b\u2009=\u2009macrophages, LSEC\u2009=\u2009Liver sinusoidal endothelial cells, HEP\u2009=\u2009Hepatocytes and HSCs\u2009=\u2009Hepatic stellate cells. 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Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-41202410485412.jpg","caption":"Increased cell-cell adhesion within one cell population is required for the formation of tightly packed cell clusters.A) Simulation of cell-cell segregation using the same adhesion term in both cell types (Aeph \u200a=\u200a Aephrin \u200a=\u200a100, left panel) vs. increased adhesion only in the green cell population (Aeph \u200a=\u200a110, Aephrin \u200a=\u200a100, right panel). Both simulations started with the same number of Eph (green) and ephrin (black) expressing cells. In the \u201cEqual adhesion\u201d case, an \u2018Islands-in-a-sea\u2019 pattern is less apparent. B) Representative images from segregation assays of unlabelled ephrin-B1 cells co-cultured with Cell Tracker-green labelled (green staining) EphB2 cells, without (left) or with E-cadherin-cherry expression (red staining, right); scale bar, 75 \u00b5m. C) Quantitation of cell densities in the cell clusters shown in B (n\u200a=\u200a10). D) Western blot analysis of lysates from parental and E-cadherin-cherry-transduced cells, using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111803), licensed under a CC-BY license. 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[Unconjugated]</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(63 Publications)</span></a></div><div class="ds_info float_left"><a href="#ReviewsSection"><div class="dsSprite_stars4"></div></a> <a href="#ReviewsSection" class="bottom0"><span class="ctr">(5 Reviews)</span></a></div><div class="ds_info float_left"><a href="/proteinsimple" title="Learn more about Simple Western"><div class="sprite_swBadge simple"> </div></a></div><div class="ds_info float_left"> <a href="/common-name/ephb2"><div class="sprite_allRelated"></div></a> <a href="/common-name/ephb2" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/AF467"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/AF467" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="5" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Western_Blot_21511.jpg" class="top_images" rel="gallery" title="Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." > <img src="//images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg" class="big_thumb" alt="Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." title="Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." /> </a> </div> <div class="alt-caption padding_left5" >Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Flow_Cytometry_20082.jpg" class="top_images" rel="gallery" title="COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." > <img src="//images.novusbio.com/images2/EphB2_AF467_Flow_Cytometry_20082.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." title="COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." /> </a> </div> <div class="alt-caption padding_left5" >COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Flow_Cytometry_20083.jpg" class="top_images" rel="gallery" title="D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." > <img src="//images.novusbio.com/images2/EphB2_AF467_Flow_Cytometry_20083.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." title="D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins." /> </a> </div> <div class="alt-caption padding_left5" >D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg" class="top_images" rel="gallery" title="EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > <img src="//images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." /> </a> </div> <div class="alt-caption padding_left5" >EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room<a class="openGallery" href="#" data-picnum="3"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Immunohistochemistry_6842.jpg" class="top_images" rel="gallery" title="EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." > <img src="//images.novusbio.com/images2/EphB2_AF467_Immunohistochemistry_6842.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." title="EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." /> </a> </div> <div class="alt-caption padding_left5" >EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at<a class="openGallery" href="#" data-picnum="4"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Immunohistochemistry_20369.jpg" class="top_images" rel="gallery" title=" EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " > <img src="//images.novusbio.com/images2/EphB2_AF467_Immunohistochemistry_20369.jpg" loading="lazy" style="display:none;" class="big_thumb" alt=" EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " title=" EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " /> </a> </div> <div class="alt-caption padding_left5" > EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467)<a class="openGallery" href="#" data-picnum="5"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Immunohistochemistry_20370.jpg" class="top_images" rel="gallery" title=" EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " > <img src="//images.novusbio.com/images2/EphB2_AF467_Immunohistochemistry_20370.jpg" loading="lazy" style="display:none;" class="big_thumb" alt=" EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " title=" EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " /> </a> </div> <div class="alt-caption padding_left5" > EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at<a class="openGallery" href="#" data-picnum="6"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/EphB2_AF467_Simple_Western_21454.jpg" class="top_images" rel="gallery" title=" Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " > <img src="//images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg" loading="lazy" style="display:none;" class="big_thumb" alt=" Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " title=" Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " /> </a> </div> <div class="alt-caption padding_left5" > Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at<a class="openGallery" href="#" data-picnum="7"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-41202410481144.jpg" class="top_images" rel="gallery" title="Colorectal tumours maintain tissue organisation similar to normal colon.Detection of EPHB2 (green) and ERBB3 (red, A and B) by co-immunofluorescence in normal colon (A) and colorectal cancer (B) (DAPI, blue). Scale bar, 50μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26367378), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-41202410481144.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Colorectal tumours maintain tissue organisation similar to normal colon.Detection of EPHB2 (green) and ERBB3 (red, A and B) by co-immunofluorescence in normal colon (A) and colorectal cancer (B) (DAPI, blue). Scale bar, 50μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26367378), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Colorectal tumours maintain tissue organisation similar to normal colon.Detection of EPHB2 (green) and ERBB3 (red, A and B) by co-immunofluorescence in normal colon (A) and colorectal cancer (B) (DAPI, blue). Scale bar, 50μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26367378), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Colorectal tumours maintain tissue organisation similar to normal colon.Detection of EPHB2 (green) and ERBB3 (red, A and B) by co-immunofluorescence in normal colon (A) and colorectal cancer (B) (DAPI, blue). Scale bar,<a class="openGallery" href="#" data-picnum="8"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-4120241049443.jpg" class="top_images" rel="gallery" title="Expression of EphB2 increases and is activated on HSCs after chronic CCl4 -induced liver injury. (a) Isolated cell fractions from livers of mice subjected to chronic CCl4 injections were analyzed for EphB2, Ephrin-B1, Ephrin-B2 and Ephrin-B3 mRNA levels using RT-qPCR. Results are shown as fold change compared to liver cell fractions obtained from vehicle-treated controls. Error bars represent mean ± SEM.; n = 6 animals; CD11b = macrophages, LSEC = Liver sinusoidal endothelial cells, HEP = Hepatocytes and HSCs = Hepatic stellate cells. (b) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle (oil) controls were stained with EphB2 (red), alpha SMA (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 100 µm, “C” denotes the central vein. All images are representative of 5 mice per group. (c) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle controls were stained with phospho-EphB1/EphB2-Y594 (red), PDGFR beta (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 50 µm. All images are representative of 5 mice per group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29416088), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-4120241049443.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Expression of EphB2 increases and is activated on HSCs after chronic CCl4 -induced liver injury. (a) Isolated cell fractions from livers of mice subjected to chronic CCl4 injections were analyzed for EphB2, Ephrin-B1, Ephrin-B2 and Ephrin-B3 mRNA levels using RT-qPCR. Results are shown as fold change compared to liver cell fractions obtained from vehicle-treated controls. Error bars represent mean ± SEM.; n = 6 animals; CD11b = macrophages, LSEC = Liver sinusoidal endothelial cells, HEP = Hepatocytes and HSCs = Hepatic stellate cells. (b) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle (oil) controls were stained with EphB2 (red), alpha SMA (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 100 µm, “C” denotes the central vein. All images are representative of 5 mice per group. (c) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle controls were stained with phospho-EphB1/EphB2-Y594 (red), PDGFR beta (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 50 µm. All images are representative of 5 mice per group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29416088), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Expression of EphB2 increases and is activated on HSCs after chronic CCl4 -induced liver injury. (a) Isolated cell fractions from livers of mice subjected to chronic CCl4 injections were analyzed for EphB2, Ephrin-B1, Ephrin-B2 and Ephrin-B3 mRNA levels using RT-qPCR. Results are shown as fold change compared to liver cell fractions obtained from vehicle-treated controls. Error bars represent mean ± SEM.; n = 6 animals; CD11b = macrophages, LSEC = Liver sinusoidal endothelial cells, HEP = Hepatocytes and HSCs = Hepatic stellate cells. (b) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle (oil) controls were stained with EphB2 (red), alpha SMA (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 100 µm, “C” denotes the central vein. All images are representative of 5 mice per group. (c) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle controls were stained with phospho-EphB1/EphB2-Y594 (red), PDGFR beta (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 50 µm. All images are representative of 5 mice per group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29416088), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Expression of EphB2 increases and is activated on HSCs after chronic CCl4 -induced liver injury. (a) Isolated cell fractions from livers of mice subjected to chronic CCl4 injections were analyzed for EphB2, Ephrin-B1,<a class="openGallery" href="#" data-picnum="9"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-41202410485412.jpg" class="top_images" rel="gallery" title="Increased cell-cell adhesion within one cell population is required for the formation of tightly packed cell clusters.A) Simulation of cell-cell segregation using the same adhesion term in both cell types (Aeph = Aephrin = 100, left panel) vs. increased adhesion only in the green cell population (Aeph = 110, Aephrin = 100, right panel). Both simulations started with the same number of Eph (green) and ephrin (black) expressing cells. In the “Equal adhesion” case, an ‘Islands-in-a-sea’ pattern is less apparent. B) Representative images from segregation assays of unlabelled ephrin-B1 cells co-cultured with Cell Tracker-green labelled (green staining) EphB2 cells, without (left) or with E-cadherin-cherry expression (red staining, right); scale bar, 75 µm. C) Quantitation of cell densities in the cell clusters shown in B (n = 10). D) Western blot analysis of lysates from parental and E-cadherin-cherry-transduced cells, using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111803), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/af467_human-mouse-ephb2-affinity-purified-polyclonal-ab-41202410485412.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Increased cell-cell adhesion within one cell population is required for the formation of tightly packed cell clusters.A) Simulation of cell-cell segregation using the same adhesion term in both cell types (Aeph = Aephrin = 100, left panel) vs. increased adhesion only in the green cell population (Aeph = 110, Aephrin = 100, right panel). Both simulations started with the same number of Eph (green) and ephrin (black) expressing cells. In the “Equal adhesion” case, an ‘Islands-in-a-sea’ pattern is less apparent. B) Representative images from segregation assays of unlabelled ephrin-B1 cells co-cultured with Cell Tracker-green labelled (green staining) EphB2 cells, without (left) or with E-cadherin-cherry expression (red staining, right); scale bar, 75 µm. C) Quantitation of cell densities in the cell clusters shown in B (n = 10). D) Western blot analysis of lysates from parental and E-cadherin-cherry-transduced cells, using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111803), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Increased cell-cell adhesion within one cell population is required for the formation of tightly packed cell clusters.A) Simulation of cell-cell segregation using the same adhesion term in both cell types (Aeph = Aephrin = 100, left panel) vs. increased adhesion only in the green cell population (Aeph = 110, Aephrin = 100, right panel). Both simulations started with the same number of Eph (green) and ephrin (black) expressing cells. In the “Equal adhesion” case, an ‘Islands-in-a-sea’ pattern is less apparent. B) Representative images from segregation assays of unlabelled ephrin-B1 cells co-cultured with Cell Tracker-green labelled (green staining) EphB2 cells, without (left) or with E-cadherin-cherry expression (red staining, right); scale bar, 75 µm. C) Quantitation of cell densities in the cell clusters shown in B (n = 10). D) Western blot analysis of lysates from parental and E-cadherin-cherry-transduced cells, using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111803), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Increased cell-cell adhesion within one cell population is required for the formation of tightly packed cell clusters.A) Simulation of cell-cell segregation using the same adhesion term in both cell types (Aeph = <a class="openGallery" href="#" data-picnum="10"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span>, <span title='Mouse'>Mu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Simple Western'>Simple Western</span>, <span title='Flow Cytometry'>Flow</span>, <span title='Immunohistochemistry'>IHC</span>, <span title='CyTOF-ready'>CyTOF-ready</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Goat</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="Human/Mouse EphB2 Affinity Purified Polyclonal Ab" data-catnum="AF467" data-catnums="AF467,AF467-SP" data-disabled=''><noscript><form autocomplete="off" action="/products/ephb2-antibody_af467" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # AF467-SP">25 ug</div><div data-msg='' class="atc_catnum">AF467-SP</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">8-10 Business Days</td> </tr> <tr class="odd"><td><div class="atc_size" title="catalog # AF467">100 ug</div><div data-msg='' class="atc_catnum">AF467</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">4-6 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-iNGG2uinULYpOmQ1MSrVmWGHHkp-EiEf-hXJ4ZbbFY4" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"><div class="list_expand options_closed" data-name="Conjugates" data-wrapper-type=".conjugates_wrapper"><div class="options_text">View Available Conjugates</div> <div class="expander option_closed"></div></div><div class="conjugates_wrapper" data-title="EphB2 Antibody [Unconjugated]" data-catnum="AF467"><table class="scf_table"> <thead> <tr> <th>Catalog# & Conjugate</th> <th>Size</th> <th class="col_price">Price</th> <th class="col_cart hidden"></th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="BAF467"> <td> <a href="/products/ephb2-antibody_baf467" class="conj_link">Biotin</a><br /> <a href="/products/ephb2-antibody_baf467" class="cat_link">BAF467</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a 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part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"><table class="scf_table"> <thead> <tr> <th>Conjugate</th> <th>Catalog #</th> <th class="head_avail">Availability</th> <th>Size</th> <th class="col_price">Price</th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="BAF467"> <td><a href="/products/ephb2-antibody_baf467">Biotin</a></td> <td><a href="/products/ephb2-antibody_baf467">BAF467</a></td> <td><div class="popup_stock"></div></td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> </tr> <tr class="scf_footer"> <td colspan="5"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["BAF467"]' data-rendered="false"></div></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>EphB2 Antibody [Unconjugated] Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>Mouse myeloma cell line NS0-derived recombinant mouse EphB2<br />Val27-Lys548<br />Accession # P54763</div></td> </tr><tr class="lined"> <td><strong>Specificity</strong></td> <td><div>Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.</div></td> </tr><tr class="lined"> <td><strong>Source</strong></td> <td><div>N/A</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Goat</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>EPHB2</div></td> </tr><tr class="lined"> <td><strong>Purity Statement</strong></td> <td><div>Antigen Affinity-purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>CyTOF-ready </li><li>Flow Cytometry 0.25 ug/10^6 cells</li><li>Immunocytochemistry 5-15 ug/mL</li><li>Immunohistochemistry 1-15 ug/mL</li><li>Simple Western 20 ug/mL</li><li>Western Blot 0.1 ug/mL</li> </ul> </td> </tr><tr class="lined"> <td><strong>Reviewed Applications</strong></td> <td> <table class="wide"> <tr> <td> Read <a href="#ReviewsSection">5 Reviews</a> rated 4.2<div class="dsSprite_stars4"></div> using <br/>AF467 in the following applications:<br /><br /> <ul class="information-list"><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Western Blot">Western Blot</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunoprecipitation">Immunoprecipitation</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Simple Western">Simple Western</a></div> <div class="information-data"></div> </li> </ul> </td> </tr> </table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> AF467 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Functional Assay">Functional Assay</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ICC">ICC</a> </div><div class="information-data">9 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IF">IF</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC">IHC</a> </div><div class="information-data">10 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-Fr">IHC-Fr</a> </div><div class="information-data">4 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-P">IHC-P</a> </div><div class="information-data">6 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunoprecipitation">Immunoprecipitation</a> </div><div class="information-data">9 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="In Vivo">In Vivo</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Neutralization">Neutralization</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Simple Western">Simple Western</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Western Blot">Western Blot</a> </div><div class="information-data">20 publications</div></li></ul></td> </tr> </table> </td> </tr> </table> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>No Preservative</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr><tr class="lined"> <td><strong>Reconstitution Instructions</strong></td> <td><div>Reconstitute at 0.2 mg/mL in sterile PBS.</div></td> </tr></table></div> <h2 class="greyHead2">Notes</h2><div class="ds_info"> <a id="tab1notes"></a> <div>This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.</div> </div><h2 class="greyHead2">Alternate Names for EphB2 Antibody [Unconjugated]</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>CAPB</li><li>Cek5</li><li>Drt</li><li>DRTEphB2</li><li>EC 2.7.10</li><li>EC 2.7.10.1</li><li>EK5</li><li>elk-related tyrosine kinase</li><li>EPH receptor B2</li><li>eph tyrosine kinase 3</li><li>EphB2</li><li>EPH-like kinase 5</li><li>ephrin type-B receptor 2</li><li>EPHT3MGC87492</li><li>EPTH3</li><li>Erk</li><li>ERKHek5</li><li>Hek5</li><li>Nuk</li><li>PCBC</li><li>protein-tyrosine kinase HEK5</li><li>Qek2</li><li>Renal carcinoma antigen NY-REN-47</li><li>Sek3</li><li>Tyro5</li><li>Tyrosine-protein kinase receptor EPH-3</li><li>Tyrosine-protein kinase TYRO5</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div><p>EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or<br>Fc‑clustered ligands are capable of activating the receptor <em>in vitro</em>. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). <em>In vivo</em>, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).</p><div class="backref"><OL> <LI><EM>Eph Nomenclature Committee [letter]</EM> (1997) Cell <STRONG>90:</STRONG>403.</LI> <LI>Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. <STRONG>21</STRONG>:309.</LI> <LI>Pasquale, E.B. (1997) Curr. Opin. Cell Biol. <STRONG>9:</STRONG>608.</LI></OL></div></div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-22203"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk1-antibody-1e5_nbp2-22203" style="overflow: hidden; width: 280px;" title="ERK1 Antibody (1E5) - BSA Free">ERK1 Antibody<br> (1E5) - BSA Free</a>    </div><div class="sub-header">NBP2-22203</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red)." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg" alt="Flow Cytometry ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). 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" class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" alt="Immunohistochemistry ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" alt="Knockout Validated ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk2-antibody_af1230#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB17761"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/jnk1-antibody-228601_mab17761" style="overflow: hidden; width: 280px;" title="JNK1 Antibody (228601) [Unconjugated]">JNK1 Antibody<br> (228601) [Unconjugate</a>    </div><div class="sub-header">MAB17761</div> <div class="images_customers"><div id="ss_prev_cav2" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev_cav2" data-cycle-next="#ss_next_cav2" data-cycle-slides="div.big_thumb_cust_container" data-index=2 ><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg" alt="Western Blot JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg" alt="Western Blot JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="JNK1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg" alt="Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/jnk1-antibody-228601_mab17761#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/jnk1-antibody-228601_mab17761#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF887"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/akt-antibody_af887" style="overflow: hidden; width: 280px;" title="AKT [p Ser473] Antibody [Unconjugated] - Pan Specific">AKT [p Ser473]<br> Antibody [Unconjugate ...</a>    </div><div class="sub-header">AF887</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/akt-antibody_af887" title="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg" alt="Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/akt-antibody_af887" title="Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/16332.jpg" alt="Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/akt-antibody_af887" title="Serum-starved MCF-7 human breast cancer cells were unstimulated (blue open histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). To facilitate intracellular staining, cells were fixed with formaldehyde (FC004) and permeabilized with Methanol." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg" alt="Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/akt-antibody_af887#PublicationSection"> <div class="sprite_pubBook"></div> (50 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, IHC, ICFlow, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF8691"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/p38-alpha-antibody_af8691" style="overflow: hidden; width: 280px;" title="p38 alpha Antibody [Unconjugated]">p38 alpha Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF8691</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/p38-alpha-antibody_af8691" title="Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for p38 alpha at approximately 43&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the12-230 kDa separation system." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/16324.jpg" alt="Simple Western p38 alpha Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p38-alpha-antibody_af8691" title="Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38a knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for p38a at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/p38_alpha_AF8691_Western_Blot_22371.jpg" alt="Western Blot p38 alpha Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p38-alpha-antibody_af8691" title="Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; MAB734), Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights&amp;#8482; 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/p38_alpha_AF8691_Immunohistochemistry_7087.jpg" alt="Immunohistochemistry p38 alpha Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/p38-alpha-antibody_af8691#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/p38-alpha-antibody_af8691#PublicationSection"> <div class="sprite_pubBook"></div> (11 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-37568"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/crk-antibody-3g11e8_nbp2-37568" style="overflow: hidden; width: 280px;" title="Crk Antibody (3G11E8) - BSA Free">Crk Antibody<br> (3G11E8) - BSA Free</a>    </div><div class="sub-header">NBP2-37568</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/crk-antibody-3g11e8_nbp2-37568" title="ELISA: Crk Antibody (3G11E8) [NBP2-37568] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);" class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Crk-Antibody-3G11E8-ELISA-NBP2-37568-img0001.jpg" alt="ELISA Crk Antibody (3G11E8) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/crk-antibody-3g11e8_nbp2-37568" title="Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)" class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Crk-Antibody-3G11E8-Western-Blot-NBP2-37568-img0002.jpg" alt="Western Blot Crk Antibody (3G11E8) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/crk-antibody-3g11e8_nbp2-37568" title="Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Crk-Antibody-3G11E8-Western-Blot-NBP2-37568-img0003.jpg" alt="Western Blot Crk Antibody (3G11E8) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-00764"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/grap2-antibody-oti1g2_nbp2-00764" style="overflow: hidden; width: 280px;" title="GRAP2 Antibody (OTI1G2)">GRAP2 Antibody<br> (OTI1G2)</a>    </div><div class="sub-header">NBP2-00764</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/grap2-antibody-oti1g2_nbp2-00764" title="Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - Analysis of Jurkat cells, using anti-GRAP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue)." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/GRAP2-Antibody-1G2-Flow-Cytometry-NBP2-00764-img0001.jpg" alt="Flow Cytometry GRAP2 Antibody (OTI1G2)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/grap2-antibody-oti1g2_nbp2-00764" title="Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRAP2 antibody, and then analyzed by flow cytometry." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/GRAP2-Antibody-1G2-Flow-Cytometry-NBP2-00764-img0002.jpg" alt="Flow Cytometry GRAP2 Antibody (OTI1G2)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/grap2-antibody-oti1g2_nbp2-00764" title="Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRAP2." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/GRAP2-Antibody-1G2-Western-Blot-NBP2-00764-img0003.jpg" alt="Western Blot GRAP2 Antibody (OTI1G2)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1347"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/p38-gamma-sapk3-antibody_af1347" style="overflow: hidden; width: 280px;" title="p38 gamma/SAPK3 Antibody [Unconjugated]">p38 gamma/SAPK3<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF1347</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/p38-gamma-sapk3-antibody_af1347" title="p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/p38_gamma_AF1347_Immunocytochemistry__Immunofluorescence_21057.jpg" alt="Immunocytochemistry p38 gamma/SAPK3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p38-gamma-sapk3-antibody_af1347" title="Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for p38 gamma at approximately 48&amp;nbsp;kDa (as indicated) using 2&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system. " class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/p38_gamma_AF1347_Simple_Western_16621.jpg" alt="Simple Western p38 gamma/SAPK3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p38-gamma-sapk3-antibody_af1347" title="Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/p38_gamma_AF1347_Western_Blot_12767.jpg" alt="Western Blot p38 gamma/SAPK3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/p38-gamma-sapk3-antibody_af1347#PublicationSection"> <div class="sprite_pubBook"></div> (8 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC, IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-81575"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/aimp2-antibody_nbp1-81575" style="overflow: hidden; width: 280px;" title="AIMP2 Antibody">AIMP2 Antibody</a>    </div><div class="sub-header">NBP1-81575</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/aimp2-antibody_nbp1-81575" title="Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0009.jpg" alt="Immunohistochemistry-Paraffin AIMP2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/aimp2-antibody_nbp1-81575" title="Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0010.jpg" alt="Immunohistochemistry-Paraffin AIMP2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/aimp2-antibody_nbp1-81575" title="Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0011.jpg" alt="Immunohistochemistry-Paraffin AIMP2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00010598-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ahsa1-antibody-1a2-a8_h00010598-m01" style="overflow: hidden; width: 280px;" title="AHSA1 Antibody (1A2-A8)">AHSA1 Antibody<br> (1A2-A8)</a>    </div><div class="sub-header">H00010598-M01</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/ahsa1-antibody-1a2-a8_h00010598-m01" title="Western Blot: AHSA1 Antibody (1A2-A8) [H00010598-M01] - AHSA1 monoclonal antibody (M01), clone 1A2-A8 Analysis of AHSA1 expression in HepG2." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/AHSA1-Antibody-1A2-A8-Western-Blot-H00010598-M01-img0004.jpg" alt="Western Blot AHSA1 Antibody (1A2-A8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ahsa1-antibody-1a2-a8_h00010598-m01" title="Sandwich ELISA: AHSA1 Antibody (1A2-A8) [H00010598-M01] - Detection limit for recombinant GST tagged AHSA1 is 3 ng/ml as a capture antibody." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/AHSA1-Antibody-1A2-A8-Sandwich-ELISA-H00010598-M01-img0003.jpg" alt="Sandwich ELISA AHSA1 Antibody (1A2-A8)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/ahsa1-antibody-1a2-a8_h00010598-m01#PublicationSection"> <div class="sprite_pubBook"></div> (6 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, S-ELISA, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-68874"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/poldip2-antibody_nbp1-68874" style="overflow: hidden; width: 280px;" title="POLDIP2 Antibody">POLDIP2 Antibody</a>    </div><div class="sub-header">NBP1-68874</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/poldip2-antibody_nbp1-68874" title="Western Blot: POLDIP2 Antibody [NBP1-68874] - Staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/POLDIP2-Antibody-Western-Blot-NBP1-68874-img0006.jpg" alt="Western Blot POLDIP2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/poldip2-antibody_nbp1-68874" title="Western Blot: POLDIP2 Antibody [NBP1-68874] - (1ug/ml) Analysis of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/POLDIP2-Antibody-Western-Blot-NBP1-68874-img0005.jpg" alt="Western Blot POLDIP2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/poldip2-antibody_nbp1-68874#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> PEP-ELISA, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB8930"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/c-jun-antibody-1018y_mab8930" style="overflow: hidden; width: 280px;" title="c-jun [p Ser63] Antibody (1018Y) [Unconjugated]">c-jun [p Ser63]<br> Antibody (1018Y) [Unc ...</a>    </div><div class="sub-header">MAB8930</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c-Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg" alt="Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="c-Jun phosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg" alt="Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV)&amp;nbsp;with a&amp;nbsp;30 minute recovery, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-c‑Jun&amp;nbsp;(S63)&amp;nbsp;at approximately 49&amp;nbsp;kDa (as indicated) using&amp;nbsp;5 μg/mL&amp;nbsp;of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) . This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg" alt="Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/c-jun-antibody-1018y_mab8930#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/c-jun-antibody-1018y_mab8930#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-47833"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" style="overflow: hidden; width: 280px;" title="MEK1 Antibody (OTI1F5)">MEK1 Antibody<br> (OTI1F5)</a>    </div><div class="sub-header">NBP1-47833</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" title="Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK1." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/MEK1-Antibody-1F5-Western-Blot-NBP1-47833-img0001.jpg" alt="Western Blot MEK1 Antibody (OTI1F5)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" title="Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0002.jpg" alt="Immunohistochemistry-Paraffin MEK1 Antibody (OTI1F5)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" title="Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0003.jpg" alt="Immunohistochemistry-Paraffin MEK1 Antibody (OTI1F5)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="210-TA"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" style="overflow: hidden; width: 280px;" title="TNF-alpha [Unconjugated]">TNF-alpha<br> [Unconjugated]</a>    </div><div class="sub-header">210-TA</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" alt="SDS-PAGE TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg" alt="Bioactivity TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg" alt="SEC-MALS TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#PublicationSection"> <div class="sprite_pubBook"></div> (820 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-13304"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/sgsm3-antibody_nbp2-13304" style="overflow: hidden; width: 280px;" title="SGSM3 Antibody">SGSM3 Antibody</a>    </div><div class="sub-header">NBP2-13304</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/sgsm3-antibody_nbp2-13304" title="Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/SGSM3-Antibody-Immunohistochemistry-Paraffin-NBP2-13304-img0001.jpg" alt="Immunohistochemistry-Paraffin SGSM3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sgsm3-antibody_nbp2-13304" title="Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/SGSM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13304-img0005.jpg" alt="Immunocytochemistry/ Immunofluorescence SGSM3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sgsm3-antibody_nbp2-13304" title="Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/SGSM3-Antibody-Western-Blot-NBP2-13304-img0004.jpg" alt="Western Blot SGSM3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF231"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/egfr-antibody_af231" style="overflow: hidden; width: 280px;" title="EGFR Antibody [Unconjugated]">EGFR Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF231</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/egfr-antibody_af231" title=" EGFR was detected in immersion fixed&amp;nbsp;frozen sections of human&amp;nbsp;skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg" alt="Immunohistochemistry EGFR Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/egfr-antibody_af231" title="EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg" alt="Immunocytochemistry EGFR Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/egfr-antibody_af231" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg" alt="Western Blot EGFR Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/egfr-antibody_af231#PublicationSection"> <div class="sprite_pubBook"></div> (30 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB4540"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/raf-1-antibody-563002_mab4540" style="overflow: hidden; width: 280px;" title="Raf-1 Antibody (563002) [Unconjugated]">Raf-1 Antibody<br> (563002) [Unconjugate</a>    </div><div class="sub-header">MAB4540</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/raf-1-antibody-563002_mab4540" title="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf-1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Raf-1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/Raf-1_MAB4540_Western_Blot_9426.jpg" alt="Western Blot Raf-1 Antibody (563002) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/raf-1-antibody-563002_mab4540" title="Raf-1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf-1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/Raf-1_MAB4540_Immunohistochemistry_9891.jpg" alt="Immunohistochemistry Raf-1 Antibody (563002) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/raf-1-antibody-563002_mab4540" title="Simple Western lane view shows lysates of MCF&amp;#x2011;7 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Raf&amp;#x2011;1 at approximately 75&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of Mouse Anti-Human/Mouse/Rat Raf&amp;#x2011;1 Monoclonal Antibody (Catalog # MAB4540) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/mab4540_human-mouse-rat-raf-1-mab-clone-563002-simple-western-158202212919.jpg" alt="Simple Western Raf-1 Antibody (563002) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/raf-1-antibody-563002_mab4540#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF467"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ephb2-antibody_af467" style="overflow: hidden; width: 280px;" title="EphB2 Antibody [Unconjugated]">EphB2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF467</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="EphB2 Antibody [Unconjugated]" class="cust_viewed_17"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="EphB2 Antibody [Unconjugated]" title="EphB2 Antibody [Unconjugated]" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for EphB2 Antibody (AF467)(63)</h2><div class="ds_info"> <div>We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.<br /><br />We have publications tested in 11 applications: Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Neutralization, Simple Western, Western Blot.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Functional Assay"> <div style="display:inline;">Functional Assay</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ICC"> <div style="display:inline;">ICC</div> (9)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IF"> <div style="display:inline;">IF</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC"> <div style="display:inline;">IHC</div> (10)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-Fr"> <div style="display:inline;">IHC-Fr</div> (4)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-P"> <div style="display:inline;">IHC-P</div> (6)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Immunoprecipitation"> <div style="display:inline;">Immunoprecipitation</div> (9)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="In Vivo"> <div style="display:inline;">In Vivo</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Neutralization"> <div style="display:inline;">Neutralization</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Simple Western"> <div style="display:inline;">Simple Western</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Western Blot"> <div style="display:inline;">Western Blot</div> (20)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (17)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (25)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (3)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Xenopus"> <div style="display:inline;">Xenopus</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">10</span> of 63. <a class="pubs_showall">Show All 63 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a><table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using AF467</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="Western Blot" data-species="Human, Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/39532838" target="_blank" rel="nofollow"> Okagawa, S;Sakaguchi, M;Okubo, Y;Takekuma, Y;Igata, M;Kondo, T;Takeda, N;Araki, K;Brandao, BB;Qian, WJ;Tseng, YH;Kulkarni, RN;Kubota, N;Kahn, CR;Araki, E; Hepatic SerpinA1 improves energy and glucose metabolism through regulation of preadipocyte proliferation and UCP1 expression Nature communications 2024-11-12 [PMID: 39532838] (Western Blot, Human, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human, Mouse</td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species="Human, Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37693478" target="_blank" rel="nofollow"> Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A; Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693478] (Western Blot, Human, Rat) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human, Rat</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37488910" target="_blank" rel="nofollow"> Michael W. Weible Ii, Michael D. Lovelace, Hamish D. Mundell, Tsz Wai Rosita Pang, Tailoi Chan-Ling BMPRII+ neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development Neural Regeneration Research 2/1/2024 [PMID: 37488910] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Immunoprecipitation" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37149869" target="_blank" rel="nofollow"> Gonz�lez-Gonz�lez, IM;Gray, JA;Ferreira, J;Conde-Dusman, MJ;Bouchet, D;Perez-Ota�o, I;Groc, L; GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain development Cell reports 2023-05-06 [PMID: 37149869] (Immunoprecipitation, Mouse) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35949596" target="_blank" rel="nofollow"> Y Suzuki, K Okabayashi, H Hasegawa, M Tsuruta, R Seishima, T Tokuda, Y Kitagawa Role of EphB2/ephrin-B1 signalling in the development and progression of obesity-associated colorectal cancer Oncology Letters, 2022-07-19;24(3):316. 2022-07-19 [PMID: 35949596] (IHC, Human) </a></td> <td valign="top">IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35831497" target="_blank" rel="nofollow"> M Azkanaz, B Corominas-, SIJ Ellenbroek, L Bruens, AT Webb, D Laskaris, KC Oost, SJA Lafirenze, K Annusver, HA Messal, S Iqbal, DJ Flanagan, DJ Huels, F Rojas-Rodr, M Vizoso, M Kasper, OJ Sansom, HJ Snippert, P Liberali, BD Simons, P Katajisto, E Hannezo, J van Rheene Retrograde movements determine effective stem cell numbers in the intestine Nature, 2022-07-13;0(0):. 7/13/2022 [PMID: 35831497] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35793621" target="_blank" rel="nofollow"> SL Banerjee, F Lessard, FJM Chartier, K Jacquet, AI Osornio-He, V Teyssier, K Ghani, N Lavoie, JN Lavoie, M Caruso, P Laprise, S Elowe, JP Lambert, N Bisson EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks Cell Reports, 2022-07-05;40(1):111031. 2022-07-05 [PMID: 35793621] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35427568" target="_blank" rel="nofollow"> Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury Experimental Eye Research 6/1/2022 [PMID: 35427568] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IF" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35294885" target="_blank" rel="nofollow"> AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse) </a></td> <td valign="top">IF</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34831152" target="_blank" rel="nofollow"> E López-Arri, B Yan, T Lobo-Jarne, Y Guillén, S Menéndez, M Andreu, A Bigas, M Iglesias, L Espinosa Accumulation of Paneth Cells in Early Colorectal Adenomas Is Associated with Beta-Catenin Signaling and Poor Patient Prognosis Cells, 2021-10-28;10(11):. 2021-10-28 [PMID: 34831152] (IHC, Human) </a></td> <td valign="top">IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34634301" target="_blank" rel="nofollow"> L M Gagné, N Morin, N Lavoie, N Bisson, JP Lambert, FA Mallette, MÉ Huot Tyrosine phosphorylation of DEPTOR functions as a molecular switch to activate mTOR signaling The Journal of Biological Chemistry, 2021-10-09;297(5):101291. 2021-10-09 [PMID: 34634301] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34462781" target="_blank" rel="nofollow"> Zhimin Huang, Simeng Liu, Anna Tang, Laith Al-Rabadi, Mark Henkemeyer, Patrice N. Mimche et al. Key role for EphB2 receptor in kidney fibrosis Clinical Science 9/17/2021 [PMID: 34462781] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33661095" target="_blank" rel="nofollow"> Jing Zhou, Yong Lin, Trung Huynh, Hirofumi Noguchi, Jeffrey O Bush, Samuel J Pleasure NMDA receptors control development of somatosensory callosal axonal projections eLife 3/4/2021 [PMID: 33661095] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33627480" target="_blank" rel="nofollow"> MS Ahmed, P Wang, NUN Nguyen, Y Nakada, I Menendez-M, M Ismail, R Bachoo, M Henkemeyer, HA Sadek, ES Kandil Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain Proceedings of the National Academy of Sciences of the United States of America, 2021-03-09;118(10):. 2021-03-09 [PMID: 33627480] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="In Vivo" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32404061" target="_blank" rel="nofollow"> A Kischel, C Audouard, MA Fawal, A Davy Ephrin-B2 paces neuronal production in the developing neocortex BMC Dev. Biol., 2020-05-13;20(1):12. 2020-05-13 [PMID: 32404061] (In Vivo, Mouse) </a></td> <td valign="top">In Vivo</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32396863" target="_blank" rel="nofollow"> C Morral, J Stanisavlj, X Hernando-M, E Mereu, A Álvarez-Va, C Cortina, D Stork, F Slebe, G Turon, G Whissell, M Sevillano, A Merlos-Suá, À Casanova-M, C Moutinho, SW Lowe, LE Dow, A Villanueva, E Sancho, H Heyn, E Batlle Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer Cell Stem Cell, 2020-05-11;26(6):845-861.e12. 5/11/2020 [PMID: 32396863] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32366833" target="_blank" rel="nofollow"> ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020-05-04;11(5):307. 2020-05-04 [PMID: 32366833] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32337793" target="_blank" rel="nofollow"> D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands J. Leukoc. Biol., 2020-04-26;0(0):. 2020-04-26 [PMID: 32337793] (IHC, Human) </a></td> <td valign="top">IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32249832" target="_blank" rel="nofollow"> S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020-04-06;10(1):5989. 2020-04-06 [PMID: 32249832] (ICC, Human) </a></td> <td valign="top">ICC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32004754" target="_blank" rel="nofollow"> L Farr, S Ghosh, N Jiang, K Watanabe, M Parlak, R Bucala, S Moonah CD74 signaling links inflammation to intestinal epithelial cell regeneration and promotes mucosal healing Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):. 2020-01-28 [PMID: 32004754] (IHC, Mouse) </a></td> <td valign="top">IHC</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Functional Assay, ICC" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31996679" target="_blank" rel="nofollow"> HR Washburn, NL Xia, W Zhou, YT Mao, MB Dalva Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility Nat Commun, 2020-01-29;11(1):570. 2020-01-29 [PMID: 31996679] (Functional Assay, ICC, Mouse) </a></td> <td valign="top">Functional Assay, ICC</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31805710" target="_blank" rel="nofollow"> YL Liu, AM Horning, B Lieberman, M Kim, CK Lin, CN Hung, CW Chou, CM Wang, CL Lin, NB Kirma, MA Liss, R Vasisht, EP Perillo, K Blocher, H Horng, JA Taverna, J Ruan, TE Yankeelov, AK Dunn, TH Huang, HC Yeh, CL Chen Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer Cancers (Basel), 2019-12-01;11(12):. 2019-12-01 [PMID: 31805710] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation, Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31577955" target="_blank" rel="nofollow"> Z Zhang, M Ye, Q Li, Y You, H Yu, Y Ma, L Mei, X Sun, L Wang, W Yue, R Li, J Li, D Zhang The Schizophrenia Susceptibility Gene OPCML Regulates Spine Maturation and Cognitive Behaviors through Eph-Cofilin Signaling Cell Rep, 2019-10-01;29(1):49-61.e7. 2019-10-01 [PMID: 31577955] (Immunoprecipitation, Western Blot, Mouse) </a></td> <td valign="top">Immunoprecipitation, Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31075258" target="_blank" rel="nofollow"> Zhonglin Wu, Tim G. Ashlin, Qiling Xu, David G. Wilkinson Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation Experimental Cell Research 8/1/2019 [PMID: 31075258] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Simple Western" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/30722785" target="_blank" rel="nofollow"> AS Ernst, LI Böhler, AM Hagenston, A Hoffmann, S Heiland, C Sticht, M Bendszus, M Hecker, H Bading, HH Marti, T Korff, R Kunze EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke Acta Neuropathol Commun, 2019-02-05;7(1):15. 2019-02-05 [PMID: 30722785] (Simple Western, Mouse) </a></td> <td valign="top">Simple Western</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="ICC, IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/29416088" target="_blank" rel="nofollow"> PN Mimche, CM Lee, SM Mimche, M Thapa, A Grakoui, M Henkemeyer, TJ Lamb EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells Sci Rep, 2018-02-07;8(1):2532. 2018-02-07 [PMID: 29416088] (ICC, IHC-P, Mouse) </a></td> <td valign="top">ICC, IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28957681" target="_blank" rel="nofollow"> Clements MP, Byrne E, Camarillo Guerrero LF et al. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. Neuron. 9/27/2017 [PMID: 28957681] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28943029" target="_blank" rel="nofollow"> S Miura, A Suzuki Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming Cell Stem Cell, 2017-09-21;0(0):. 2017-09-21 [PMID: 28943029] (IHC, Mouse) </a></td> <td valign="top">IHC</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28931592" target="_blank" rel="nofollow"> Fumihiko Okumura, Akiko Joo-Okumura, Keisuke Obara, Alexander Petersen, Akihiko Nishikimi, Yoshinori Fukui et al. Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2 Molecular Biology of the Cell 11/15/2017 [PMID: 28931592] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="ICC, IHC-Fr" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28276447" target="_blank" rel="nofollow"> YH Liu, JW Tsai, JL Chen, WS Yang, PC Chang, PL Cheng, DL Turner, Y Yanagawa, TW Wang, JY Yu Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon Sci Rep, 2017-03-09;7(0):42895. 2017-03-09 [PMID: 28276447] (ICC, IHC-Fr, Mouse) </a></td> <td valign="top">ICC, IHC-Fr</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28262686" target="_blank" rel="nofollow"> N Tüysüz, L van Bloois, S van den Br, H Begthel, MM Verstegen, LJ Cruz, L Hui, LJ van der La, J de Jonge, R Vries, E Braakman, E Mastrobatt, JJ Cornelisse, H Clevers, D Ten Berge Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells Nat Commun, 2017-03-06;8(0):14578. 2017-03-06 [PMID: 28262686] (IHC, Human) </a></td> <td valign="top">IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC, Western Blot" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27881781" target="_blank" rel="nofollow"> Can Gao Blocking the Interaction between EphB2 and ADDLs by a Small Peptide Rescues Impaired Synaptic Plasticity and Memory Deficits in a Mouse Model of Alzheimer&#039;s Disease J. Neurosci., 2016-11-23;36(47):11959-11973. 2016-11-23 [PMID: 27881781] (ICC, Western Blot, Rat) </a></td> <td valign="top">ICC, Western Blot</td> <td valign="top">Rat</td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27819675" target="_blank" rel="nofollow"> Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells Oncogene, 2016-11-07;0(0):. 2016-11-07 [PMID: 27819675] (IHC-P, Mouse) </a></td> <td valign="top">IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27810913" target="_blank" rel="nofollow"> AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016-10-17;215(2):217-229. 2016-10-17 [PMID: 27810913] (IHC, Mouse) </a></td> <td valign="top">IHC</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC-Fr" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27646263" target="_blank" rel="nofollow"> The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model Mol Psychiatry, 2016-09-20;0(0):. 2016-09-20 [PMID: 27646263] (IHC-Fr, Mouse) </a></td> <td valign="top">IHC-Fr</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27551053" target="_blank" rel="nofollow"> Eph-mediated tyrosine phosphorylation of citron kinase controls abscission J Cell Biol, 2016-08-22;214(5):555-69. 8/22/2016 [PMID: 27551053] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27470974" target="_blank" rel="nofollow"> EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016-07-29;7(0):12329. 2016-07-29 [PMID: 27470974] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27399303" target="_blank" rel="nofollow"> Jesús Planagumà, Holger Haselmann, Francesco Mannara, Mar Petit‐Pedrol, Benedikt Grünewald, Esther Aguilar et al. Ephrin‐B2 prevents N‐methyl‐D‐aspartate receptor antibody effects on memory and neuroplasticity Annals of Neurology 9/1/2016 [PMID: 27399303] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27354374" target="_blank" rel="nofollow"> Jingyi Gong, Roman Körner, Louise Gaitanos, Rüdiger Klein Exosomes mediate cell contact–independent ephrin-Eph signaling during axon guidance Journal of Cell Biology 7/4/2016 [PMID: 27354374] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27184401" target="_blank" rel="nofollow"> Relation T, Yi T, Guess AJ et al. Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation Cell Stem Cell 5/18/2016 [PMID: 27184401] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26687224" target="_blank" rel="nofollow"> Karen Perez Perez de Arce, Nikolas Schrod, Sarah W. R. Metzbower, Edward Allgeyer, Geoffrey K.-W. Kong, Ai-Hui Tang et al. Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains Neuron 12/16/2015 [PMID: 26687224] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26589795" target="_blank" rel="nofollow"> Miyamoto T, Kim D, Knox J, Johnson E, Mucke L Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-beta-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity. J Biol Chem, 2015-11-20;291(4):1719-34. 11/20/2015 [PMID: 26589795] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26528816" target="_blank" rel="nofollow"> Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium. PLoS Genet, 2015-11-03;11(11):e1005638. 2015-11-03 [PMID: 26528816] (IHC-P, Mouse) </a></td> <td valign="top">IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25893671" target="_blank" rel="nofollow"> Maria J. Serrano, Jingpeng Liu, Kathy K.H. Svoboda, Ali Nawshad, M. Douglas Benson Ephrin reverse signaling mediates palatal fusion and epithelial-to-mesenchymal transition independently of tgf beta 3 Journal of Cellular Physiology 4/11/2018 [PMID: 25893671] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="IHC, Western Blot" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25834064" target="_blank" rel="nofollow"> Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model. J Neurosci, 2015-04-01;35(13):5409-21. 2015-04-01 [PMID: 25834064] (IHC, Western Blot, Rat) </a></td> <td valign="top">IHC, Western Blot</td> <td valign="top">Rat</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25593460" target="_blank" rel="nofollow"> Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells World Journal of Gastroenterology 1/14/2015 [PMID: 25593460] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25436892" target="_blank" rel="nofollow"> Aharon , Rotem, Janes , Peter W, Burgess , Anthony, Hamza , Kais, Klebaner , Fima, Lackmann , Martin A mathematical model for eph/ephrin-directed segregation of intermingled cells. PLoS ONE, 2014-12-01;9(12):e111803. 2014-12-01 [PMID: 25436892] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25419707" target="_blank" rel="nofollow"> Hayley Davis, Shazia Irshad, Mukesh Bansal, Hannah Rafferty, Tatjana Boitsova, Chiara Bardella et al. Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside of the crypt base stem cell niche Nature Medicine 1/1/2015 [PMID: 25419707] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="ICC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24392113" target="_blank" rel="nofollow"> Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE, 2014-01-02;9(1):e84179. 2014-01-02 [PMID: 24392113] (ICC, Human) </a></td> <td valign="top">ICC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC, Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23318582" target="_blank" rel="nofollow"> Bouche E, Romero-Ortega M, Henkemeyer M, Catchpole T, Leemhuis J, Frotscher M, May P, Herz J, Bock H Reelin induces EphB activation. Cell Res, 2013-01-15;23(4):473-90. 2013-01-15 [PMID: 23318582] (ICC, Western Blot, Mouse) </a></td> <td valign="top">ICC, Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC-Fr, Immunoprecipitation, Neutralization, Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21508957" target="_blank" rel="nofollow"> Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Neuropsin cleaves EphB2 in the amygdala to control anxiety. Nature, 2011-04-20;473(7347):372-5. 2011-04-20 [PMID: 21508957] (IHC-Fr, Immunoprecipitation, Neutralization, Western Blot, Mouse) </a></td> <td valign="top">IHC-Fr, Immunoprecipitation, Neutralization, Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC-P, Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21276420" target="_blank" rel="nofollow"> Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Biochem. Biophys. Res. Commun., 2011-01-26;405(4):521-6. 2011-01-26 [PMID: 21276420] (IHC-P, Western Blot, Mouse) </a></td> <td valign="top">IHC-P, Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/20664940" target="_blank" rel="nofollow"> P Minoo, I Zlobec, M Peterson, L Terracciano, A Lugli Characterization of rectal, proximal and distal colon cancers based on clinicopathological, molecular and protein profiles International Journal of Oncology 7/29/2010 [PMID: 20664940] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="IHC-Fr, Immunoprecipitation, Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19515977" target="_blank" rel="nofollow"> Bush JO, Soriano P Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev., 2009-06-10;23(13):1586-99. 2009-06-10 [PMID: 19515977] (IHC-Fr, Immunoprecipitation, Western Blot, Mouse) </a></td> <td valign="top">IHC-Fr, Immunoprecipitation, Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/18627264" target="_blank" rel="nofollow"> Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts. J. Bone Miner. Res., 2008-08-01;23(8):1170-81. 2008-08-01 [PMID: 18627264] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Xenopus"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/18193042" target="_blank" rel="nofollow"> Lim BK, Matsuda N, Poo MM Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo. Nat. Neurosci., 2008-01-13;11(2):160-9. 2008-01-13 [PMID: 18193042] (Immunoprecipitation, Xenopus) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Xenopus</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17591954" target="_blank" rel="nofollow"> Tanaka M, Kamata R, Takigahira M, Yanagihara K, Sakai R Phosphorylation of ephrin-B1 regulates dissemination of gastric scirrhous carcinoma. Am. J. Pathol., 2007-07-01;171(1):68-78. 2007-07-01 [PMID: 17591954] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17585030" target="_blank" rel="nofollow"> Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice. J. Nutr., 2007-07-01;137(7):1776-82. 2007-07-01 [PMID: 17585030] (IHC-P, Human) </a></td> <td valign="top">IHC-P</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17249693" target="_blank" rel="nofollow"> Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth. Biochemistry, 2007-01-24;46(7):1799-810. 2007-01-24 [PMID: 17249693] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC, Immunoprecipitation" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/16777604" target="_blank" rel="nofollow"> Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J EphB receptors coordinate migration and proliferation in the intestinal stem cell niche. Cell, 2006-06-16;125(6):1151-63. 2006-06-16 [PMID: 16777604] (IHC, Immunoprecipitation, Mouse) </a></td> <td valign="top">IHC, Immunoprecipitation</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/16443753" target="_blank" rel="nofollow"> Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 2006-02-01;119(0):559-70. 2006-02-01 [PMID: 16443753] (IHC, Mouse) </a></td> <td valign="top">IHC</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="ICC, IHC-P, Immunoprecipitation, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/16049340" target="_blank" rel="nofollow"> Nakada M, Niska JA, Tran NL, McDonough WS, Berens ME EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and invasion. Am. J. Pathol., 2005-08-01;167(2):565-76. 2005-08-01 [PMID: 16049340] (ICC, IHC-P, Immunoprecipitation, Western Blot, Human) </a></td> <td valign="top">ICC, IHC-P, Immunoprecipitation, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC, Immunoprecipitation, Neutralization, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/15126357" target="_blank" rel="nofollow"> Nakada M, Niska JA, Miyamori H, McDonough WS, Wu J, Sato H, Berens ME The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. Cancer Res., 2004-05-01;64(9):3179-85. 2004-05-01 [PMID: 15126357] (ICC, Immunoprecipitation, Neutralization, Western Blot, Human) </a></td> <td valign="top">ICC, Immunoprecipitation, Neutralization, Western Blot</td> <td valign="top">Human</td> </tr><tr><td colspan="3" align="center"> <a class="pubs_showall">Show All 63 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a> </td></tr></tbody> </table> </div> </div><a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>EphB2 Antibody (AF467)</span> (5) <span style="display:none;">4.2</span><span style="display:none;">5</span></h2> <div class="ds_info"> <div class="bottom15"> <strong>Average Rating: 4.2</strong> <div class="dsSprite_stars4"></div> (Based on 5 reviews) </div> <div><div class="ds_info">We have 5 reviews tested in 2 species: Mouse, Other.</div> <div class="align-right"> <button class="greybutton btn_width" id="novusreview" name="novusreview" onclick="window.location='/reviews/AF467'"; >Submit a Review</button><br /> </div> </div> <div class="blueHead"><strong>Reviews using AF467:</strong></div> <div class="filterParent"><div class="reviews_filterList"> <div class="blueHead">Filter by Applications</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (3)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="IP"> <div style="display:inline;">IP</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="Simple Western"> <div style="display:inline;">Simple Western</div> (1)</div><div class="filter_clear" data-type="review" data-filter="application">All Applications</div></div> <div class="reviews_filterList"> <div class="blueHead">Filter by Species</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (4)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Other"> <div style="display:inline;">Other</div> (1)</div><div class="filter_clear" data-type="review" data-filter="species">All Species</div></div></div><div id="reviews_list"> <table class="wide lightPad tablesorter" id="reviews_list_table"> <thead> <tr> <th>Images</th> <th>Ratings</th> <th>Applications</th> <th>Species</th> <th>Date</th> <th>Details</th> </tr> </thead> <tbody><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40511_0.jpg" title="Lane1: Precision Plus Protein™ Dual Color Standards7ulLane2:Ephb1 receptor (SC tissue from naive C57BL6j mouse)" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40511_0.jpg" class="reviews_imageBorder" width="75" alt="Western Blot EphB2 AF467" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="07/05/2018" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />STAVROULA THEOFANOUS</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>07/05/2018</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>brain and spinal cord</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>CFK071804A</td></tr></table><h2 class="greyHead">Comments</h2> <table class="even wide top"><tr><td class="text_bold">Comments</td><td>Blot Loading<br />• ANTIBODY: Human/Mouse EphB2Antibody cat noAF542<br />Lane1: Precision Plus Protein™ Dual Color Standards7ul<br />Lane2:Ephb1 receptor (SC tissue from naive C57BL6j mouse)<br />Western blot experiment details<br />Gel:4-20% SDS page<br />Tranfer apparatus: Trans-Blot® Turbo™ Transfer System<br />Membrane: midi size nitrocellulose<br />Time: 25min at 75-125V<br />Primary ab:Goat –rat EphB1 Ab 1mg for 20h<br />Secondary ab: rabbit a-goat 1:5,000 for 1h<br />Substrate: HRP for 15min</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IP" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40471_0_0.jpg" title="Immunoprecipitation image for AF467" class="colorbox" title="Immunoprecipitation" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40471_0_0.jpg" class="reviews_imageBorder" width="75" alt="Immunoprecipitation EphB2 AF467" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="04/16/2018" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />STAVROULA THEOFANOUS</div> </td> <td title="Immunoprecipitation">IP</td> <td>Mouse</td> <td>04/16/2018</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunoprecipitation</td></tr><tr><td class="text_bold">Sample Tested</td><td>brain and spinal cord</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>CVT0311121</td></tr></table><h2 class="greyHead">Comments</h2> <table class="even wide top"><tr><td class="text_bold">Comments</td><td>Blot Loading<br />• ANTIBODY: Human/Mouse EphB2Antibody cat noAF467<br />Lane1: PreĐision Plus Protein™ Dual Color Standards8ul<br />Lane2:Ephb2 receptors IP 1mg protein (SC tissue)<br />Lane3:Ephb2 receptors no IP 25ug (SC tissue)<br />Lane4:Goat GFP IP (negative control instead of goat IgG)1mg (SC tissue)<br />Lane5:Ephb2 receptors IP 1mg (SC tissue)<br />Lane6:Ephb2 receptors no IP 25ug (SC tissue)<br />Lane7:Goat GFP IP (negative control instead of goat IgG)1mg (SC tissue) <br />Western blot experiment details<br />Gel:4-20% SDS page<br />Tranfer apparatus: Trans-Blot® Turďo™ Transfer System<br />Membrane: midi size nitrocellulose<br />Time: 25min at 75-125V<br />Primary ab:Goat –m/h EphB2 Ab 1mg for 20h<br />Secondary ab: donkey a-goat 1:15,000 for 1h<br />Substrate: HRP for 15min </td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Excellent" data-application="Simple Western" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_34616.jpg" title="Simple Western image for AF467" class="colorbox" title="Simple Western" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_34616.jpg" class="reviews_imageBorder" width="75" alt="Simple Western EphB2 AF467" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="07/12/2017" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Simple Western">Simple Western</td> <td>Mouse</td> <td>07/12/2017</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Simple Western</td></tr><tr><td class="text_bold">Sample Tested</td><td>mouse brain lysate</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table><h2 class="greyHead">Comments</h2> <table class="even wide top"><tr><td class="text_bold">Comments</td><td>Simple Western: anti-EphB2 antibody [biotechne AF467] <br />Simple Western lane view shows a specific 128 kDa signal for EphB2 in 0.5 mg/ml of mouse brain lysate (lysis buffer from ARY014 proteome profiler kit). This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50).<br /></td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td> <meta content="01/08/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>01/08/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 21276420</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Other"> <td> <meta content="01/08/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Other</td> <td>01/08/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 22144690</td></tr><tr><td class="text_bold">Species</td><td>Other</td></tr></table> </div> </div> </td> </tr></tbody> </table> </div><br /> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/flow-cytometry/protocol.html">Flow Cytometry</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for EphB2 Antibody (AF467) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=HRP">Anti-Goat HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=FITC">Anti-Goat FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=Biotin">Anti-Goat Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Goat%22">View all of our anti-Goat Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Goat%22">Goat Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional EphB2 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array AF467</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/ephb2">EphB2 Antibodies</a></li><li> <a href="/proteins/ephb2">EphB2 Protein</a></li><li> <a href="/proteins-and-enzymes/ephb2">EphB2 Proteins and Enzymes</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on EphB2. </h2> <a id="blogsect"></a> <div class="ds_info"><a href="/antibody-news/tag/ephb2">Check out the latest blog posts on EphB2</a>.</div> <div class="ds_info"> <table id="blogs_list_table"> <tbody> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/stemness-is-responsible-for-onset-and-metastasis-of-colorectal-cancer">Stemness is responsible for onset and metastasis of colorectal cancer</a></strong><br/> <span> By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  <a href="/antibody-news/stemness-is-responsible-for-onset-and-metastasis-of-colorectal-cancer">Read full blog post.</a></span><br /><br /> </td> </tr></tbody> </table> </div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="AB-108-C"> <div class="img_container"> <a href="/products/igg-isotype-control_ab-108-c" title="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_AB108C_Control_19376.jpg" title="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." alt="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_ab-108-c">Goat IgG Isotype Control [Unconjugated]</a> <br />AB-108-C</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_ab-108-c';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="210-TA-005"> <div class="img_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." alt="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/recombinant-human-tnf-alpha-protein_210-ta">TNF-alpha [Unconjugated]</a> <br />210-TA-005</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/recombinant-human-tnf-alpha-protein_210-ta';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> 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Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_mass_unit" 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</div> </div> <h2 class="greyHead2">Recent Reviews</h2><div class="review_summary"> <div class="review_summary_left"> <div class="review_large_number">4.2</div> <div class="dsSprite_stars4"></div> </div> <div class="review_summary_right"> <table> <tbody><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">5</div></td><td style="position:relative;width:155px;"><div class="review_bar star5" style="width:25%;"></div><div class="review_bar_value">1</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">4</div></td><td style="position:relative;width:155px;"><div class="review_bar star4" style="width:100%;"></div><div class="review_bar_value">4</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">3</div></td><td style="position:relative;width:155px;"><div class="review_bar star3" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">2</div></td><td style="position:relative;width:155px;"><div class="review_bar star2" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">1</div></td><td style="position:relative;width:155px;"><div class="review_bar star1" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr> </tbody> </table> </div> <hr /><div class="single_review"><div class="review_summary_image"><a href="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40511_0.jpg" title="Lane1: Precision Plus Protein™ Dual Color Standards7ulLane2:Ephb1 receptor (SC tissue from naive C57BL6j mouse)" class="colorbox" title="Western Blot" > <img loading="lazy" src="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40511_0.jpg" class="reviews_imageBorder" width="75" alt="Western Blot EphB2 AF467" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars4" content="4"></div></td> <td>STAVROULA THEOFANOUS<br />07/05/2018</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>WB<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Mouse</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="single_review"><div class="review_summary_image"><a href="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40471_0_0.jpg" title="Immunoprecipitation image for AF467" class="colorbox" title="Immunoprecipitation" > <img loading="lazy" src="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_40471_0_0.jpg" class="reviews_imageBorder" width="75" alt="Immunoprecipitation EphB2 AF467" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars4" content="4"></div></td> <td>STAVROULA THEOFANOUS<br />04/16/2018</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>IP<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Mouse</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="single_review"><div class="review_summary_image"><a href="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_34616.jpg" title="Simple Western image for AF467" class="colorbox" title="Simple Western" > <img loading="lazy" src="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af467_34616.jpg" class="reviews_imageBorder" width="75" alt="Simple Western EphB2 AF467" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars5" content="5"></div></td> <td>Verified Customer<br />07/12/2017</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>Simple Western<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Mouse</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="align-right" style="margin-right:10px;"> <a href="#ReviewsSection"> <button class="greybutton btn_width">read all the reviews</button> </a> </div> </div><h2 class="greyHead2">Bioinformatics</h2> <div class="ds_info"> <a id="tab1bioinformatics"></a> <div> <table class="ds_list_right wide top"> <tr class="lined"> <td><strong>Gene Symbol</strong></td> <td><a href="/gene-name/EPHB2">EPHB2</a></td> </tr><tr class="grey-line-top"> <td><strong>Uniprot</strong></td> <td> 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EphB2 Antibody [Unconjugated] (AF467): Novus Biologicals