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(PDF) An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases | Celso Benedetti - Academia.edu

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We identified, by differential display, an Arabidopsis gene" /> <title>(PDF) An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases | Celso Benedetti - Academia.edu</title> <link rel="canonical" href="https://www.academia.edu/6846830/An_Arabidopsis_gene_induced_by_wounding_functionally_homologous_to_flavoprotein_oxidoreductases" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '49879c2402910372f4abc62630a427bbe033d190'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1732423194000); window.Aedu.timeDifference = new Date().getTime() - 1732423194000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flavin mononucleotide (FMN) binding proteins, including the old yellow enzyme (OYE) from yeast and 12-oxophytodienoate-10,11-reductase (OPR) from Arabidopsis. Transcripts of OPR3 rapidly accumulated in leaves after wounding and MeJA treatment, but they were detected in various tissues of unwounded plants at relatively low levels. Expression of the OPR3 gene was significantly reduced in wounded leaves of the coi1 mutant, indicating partial dependence on jasmonate perception for full induction of the gene. The recombinant protein of OPR3 cross-reacted with an antiserum raised against the OYE protein, and showed oxidation of β-NADPH when OPDA or 15-deoxy-Δ12,14-prostaglandin J2 (PGJ2), an analogue of OPDA, was used as substrate. β-NADPH oxidation was not observed when MeJA, which lacks the double bond in the ketone ring, was used as substrate. The recombinant OPR3 protein also showed β-NADPH oxidation activity in the presence of cyclohexenone, but not cyclohexanone, suggesting that the enzyme has specificity to cleavage of olefinic bonds in cyclic enones. The results show that the OPR3 gene product represents a new OPR of Arabidopsis induced after wounding.","author":[{"@context":"https://schema.org","@type":"Person","name":"Celso Benedetti"}],"contributor":[],"dateCreated":"2015-04-07","dateModified":"2015-04-07","datePublished":"2000-01-01","headline":"An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases","inLanguage":"en","keywords":[],"locationCreated":null,"publication":"Plant Molecular Biology","publisher":{"@context":"https://schema.org","@type":"Organization","name":null},"image":null,"thumbnailUrl":null,"url":"https://www.academia.edu/11833320/An_Arabidopsis_gene_induced_by_wounding_functionally_homologous_to_flavoprotein_oxidoreductases","sourceOrganization":[{"@context":"https://schema.org","@type":"EducationalOrganization","name":null}]}</script><link rel="stylesheet" media="all" 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part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flavin mononucleotide (FMN) binding proteins, including the old yellow enzyme (OYE) from yeast and 12-oxophytodienoate-10,11-reductase (OPR) from Arabidopsis. Transcripts of OPR3 rapidly accumulated in leaves after wounding and MeJA treatment, but they were detected in various tissues of unwounded plants at relatively low levels. Expression of the OPR3 gene was significantly reduced in wounded leaves of the coi1 mutant, indicating partial dependence on jasmonate perception for full induction of the gene. The recombinant protein of OPR3 cross-reacted with an antiserum raised against the OYE protein, and showed oxidation of β-NADPH when OPDA or 15-deoxy-Δ12,14-prostaglandin J2 (PGJ2), an analogue of OPDA, was used as substrate. β-NADPH oxidation was not observed when MeJA, which lacks the double bond in the ketone ring, was used as substrate. The recombinant OPR3 protein also showed β-NADPH oxidation activity in the presence of cyclohexenone, but not cyclohexanone, suggesting that the enzyme has specificity to cleavage of olefinic bonds in cyclic enones. The results show that the OPR3 gene product represents a new OPR of Arabidopsis induced after wounding.","publication_date":"2000,,","publication_name":"Plant Molecular Biology"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases","broadcastable":false,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [29198179]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;swp-splash-paper-cover&quot;,&quot;attachmentId&quot;:46496732,&quot;attachmentType&quot;:&quot;pdf&quot;}"><img alt="First page of “An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/46496732/mini_magick20190209-31204-1iph6lv.png?1549756317" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="29198179" href="https://independent.academia.edu/CelsoBenedetti"><img alt="Profile image of Celso Benedetti" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Celso Benedetti</a></div><p class="ds-work-card--detail ds2-5-body-sm">2000, Plant Molecular Biology</p><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flavin mononucleotide (FMN) binding proteins, including the old yellow enzyme (OYE) from yeast and 12-oxophytodienoate-10,11-reductase (OPR) from Arabidopsis. Transcripts of OPR3 rapidly accumulated in leaves after wounding and MeJA treatment, but they were detected in various tissues of unwounded plants at relatively low levels. Expression of the OPR3 gene was significantly reduced in wounded leaves of the coi1 mutant, indicating partial dependence on jasmonate perception for full induction of the gene. The recombinant protein of OPR3 cross-reacted with an antiserum raised against the OYE protein, and showed oxidation of β-NADPH when OPDA or 15-deoxy-Δ12,14-prostaglandin J2 (PGJ2), an analogue of OPDA, was used as substrate. β-NADPH oxidation was not observed when MeJA, which lacks the double bond in the ketone ring, was used as substrate. The recombinant OPR3 protein also showed β-NADPH oxidation activity in the presence of cyclohexenone, but not cyclohexanone, suggesting that the enzyme has specificity to cleavage of olefinic bonds in cyclic enones. The results show that the OPR3 gene product represents a new OPR of Arabidopsis induced after wounding.</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--work-card&quot;,&quot;attachmentId&quot;:46496732,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:&quot;https://www.academia.edu/11833320/An_Arabidopsis_gene_induced_by_wounding_functionally_homologous_to_flavoprotein_oxidoreductases&quot;}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--work-card&quot;,&quot;attachmentId&quot;:46496732,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:&quot;https://www.academia.edu/11833320/An_Arabidopsis_gene_induced_by_wounding_functionally_homologous_to_flavoprotein_oxidoreductases&quot;}"><span class="material-symbols-outlined" 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Métraux</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plant Physiology, 2004</p><p class="ds-related-work--abstract ds2-5-body-sm">The coordinated induced expression of β-oxidation genes is essential to provide the energy supply for germination and postgerminative development. However, very little is known about other functions of β-oxidation in nonreserve organs. We have identified a gene-specific pattern of induced β-oxidation gene expression in wounded leaves of Arabidopsis. Mechanical damage triggered the local and systemic induction of only ACX1 among acyl-coenzyme A oxidase (ACX) genes, and KAT2/PED1 among 3-ketoacyl-coenzyme A thiolase (KAT) genes in Arabidopsis. In turn, wounding induced KAT5/PKT2 only systemically. Although most of the β-oxidation genes were activated by wound-related factors such as dehydration and abscisic acid, jasmonic acid (JA) induced only ACX1 and KAT5. Reduced expression of ACX1 or KAT2 genes, in transgenic plants expressing their corresponding mRNAs in antisense orientation, correlated with defective wound-activated synthesis of JA and with reduced expression of JA-responsive ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Gene-Specific Involvement of β-Oxidation in Wound-Activated Responses in Arabidopsis&quot;,&quot;attachmentId&quot;:87897983,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/82076876/Gene_Specific_Involvement_of_%CE%B2_Oxidation_in_Wound_Activated_Responses_in_Arabidopsis&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/82076876/Gene_Specific_Involvement_of_%CE%B2_Oxidation_in_Wound_Activated_Responses_in_Arabidopsis"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="124940240" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/124940240/Photosynthesis_and_sucrose_metabolism_in_leaves_of_Arabidopsis_thaliana_aos_ein4_and_rcd1_mutants_as_affected_by_wounding">Photosynthesis and sucrose metabolism in leaves of Arabidopsis thaliana aos, ein4 and rcd1 mutants as affected by wounding</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="30910590" href="https://ledson.academia.edu/AndrzejSkoczowski">Andrzej M Skoczowski</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Acta Physiologiae Plantarum, 2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Photosynthesis and sucrose metabolism in leaves of Arabidopsis thaliana aos, ein4 and rcd1 mutants as affected by wounding&quot;,&quot;attachmentId&quot;:119072387,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/124940240/Photosynthesis_and_sucrose_metabolism_in_leaves_of_Arabidopsis_thaliana_aos_ein4_and_rcd1_mutants_as_affected_by_wounding&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/124940240/Photosynthesis_and_sucrose_metabolism_in_leaves_of_Arabidopsis_thaliana_aos_ein4_and_rcd1_mutants_as_affected_by_wounding"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="110341449" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/110341449/RESPONSE_OF_TOBACCO_POLYPHENOL_OXIDASE_GENE_TO_WOUNDING_ABSCISIC_ACID_ABA_AND_METHYL_JASMONATE_MeJ_">RESPONSE OF TOBACCO POLYPHENOL OXIDASE GENE TO WOUNDING, ABSCISIC ACID (ABA) AND METHYL JASMONATE (MeJ)</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="26286256" href="https://qau.academia.edu/ZabtaShinwari">Zabta Shinwari</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2017</p><p class="ds-related-work--abstract ds2-5-body-sm">Polyphenol oxidases (PPOs) are ubiquitous enzymes in plant kingdom and catalyze the oxidation of phenols to highly reactive quinones. PPO genes are induced by both biotic and abiotic stresses and implicated in several physiological processes including plant defense against pathogen and insects. Here, effect of wounding, abscisic acid (ABA) and methyl jasmonate (MeJ) applications on PPO induction in Nicotiana tabacum (NtPPO) was characterized by RT-PCR. In response to MeJ treatment at different concentrations, maximum expression of NtPPO genes was observed with six folds induction at 500 μM concentration, while target transcript level was relatively low (0.2-0.9 fold) with different concentrations of ABA. Upon wounding, NtPPO gene was strongly induced (up to 14 folds) after 36 hours treatment. Thus, wound and MeJ inducibility of NtPPO gene is a strong indicative of its role in plant defense mechanism against biotic and abiotic stresses.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;RESPONSE OF TOBACCO POLYPHENOL OXIDASE GENE TO WOUNDING, ABSCISIC ACID (ABA) AND METHYL JASMONATE (MeJ)&quot;,&quot;attachmentId&quot;:108188389,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/110341449/RESPONSE_OF_TOBACCO_POLYPHENOL_OXIDASE_GENE_TO_WOUNDING_ABSCISIC_ACID_ABA_AND_METHYL_JASMONATE_MeJ_&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/110341449/RESPONSE_OF_TOBACCO_POLYPHENOL_OXIDASE_GENE_TO_WOUNDING_ABSCISIC_ACID_ABA_AND_METHYL_JASMONATE_MeJ_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="14360049" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/14360049/The_NADPH_Quinone_Oxidoreductase_P1_zeta_crystallin_in_Arabidopsis_Catalyzes_the_alpha_beta_Hydrogenation_of_2_Alkenals_Detoxication_of_the_Lipid_Peroxide_Derived_Reactive_Aldehydes">The NADPH:Quinone Oxidoreductase P1-zeta-crystallin in Arabidopsis Catalyzes the alpha,beta-Hydrogenation of 2-Alkenals: Detoxication of the Lipid Peroxide-Derived Reactive Aldehydes</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33304377" href="https://ugent.academia.edu/DirkInze">Dirk Inze</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plant and Cell Physiology, 2002</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;The NADPH:Quinone Oxidoreductase P1-zeta-crystallin in Arabidopsis Catalyzes the alpha,beta-Hydrogenation of 2-Alkenals: Detoxication of the Lipid Peroxide-Derived Reactive Aldehydes&quot;,&quot;attachmentId&quot;:44284139,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/14360049/The_NADPH_Quinone_Oxidoreductase_P1_zeta_crystallin_in_Arabidopsis_Catalyzes_the_alpha_beta_Hydrogenation_of_2_Alkenals_Detoxication_of_the_Lipid_Peroxide_Derived_Reactive_Aldehydes&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/14360049/The_NADPH_Quinone_Oxidoreductase_P1_zeta_crystallin_in_Arabidopsis_Catalyzes_the_alpha_beta_Hydrogenation_of_2_Alkenals_Detoxication_of_the_Lipid_Peroxide_Derived_Reactive_Aldehydes"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="10648967" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/10648967/The_AoPR10_promoter_and_certain_endogenous_PR10_genes_respond_to_oxidative_signals_in_Arabidopsis">The AoPR10 promoter and certain endogenous PR10 genes respond to oxidative signals in Arabidopsis</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="26020131" href="https://independent.academia.edu/LMur">Luis Mur</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Molecular Plant Pathology, 2004</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" 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translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="7041682" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/7041682/Protection_against_Photooxidative_Injury_of_Tobacco_Leaves_by_2_Alkenal_Reductase_Detoxication_of_Lipid_Peroxide_Derived_Reactive_Carbonyls">Protection against Photooxidative Injury of Tobacco Leaves by 2-Alkenal Reductase. Detoxication of Lipid Peroxide-Derived Reactive Carbonyls</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="48314998" href="https://independent.academia.edu/JunIchiMano">Jun&#39;Ichi Mano</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="12024837" href="https://vub.academia.edu/LuitSlooten">Luit Slooten</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plant Physiology, 2005</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Protection against Photooxidative Injury of Tobacco Leaves by 2-Alkenal Reductase. Detoxication of Lipid Peroxide-Derived Reactive Carbonyls&quot;,&quot;attachmentId&quot;:33695994,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/7041682/Protection_against_Photooxidative_Injury_of_Tobacco_Leaves_by_2_Alkenal_Reductase_Detoxication_of_Lipid_Peroxide_Derived_Reactive_Carbonyls&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/7041682/Protection_against_Photooxidative_Injury_of_Tobacco_Leaves_by_2_Alkenal_Reductase_Detoxication_of_Lipid_Peroxide_Derived_Reactive_Carbonyls"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--sticky-ctas&quot;,&quot;attachmentId&quot;:46496732,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--sticky-ctas&quot;,&quot;attachmentId&quot;:46496732,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_46496732" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="6557978" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/6557978/Differential_Expression_of_a_Novel_Gene_in_Response_to_Coronatine_Methyl_Jasmonate_and_Wounding_in_the_Coi1_Mutant_of_Arabidopsis">Differential Expression of a Novel Gene in Response to Coronatine, Methyl Jasmonate, and Wounding in the Coi1 Mutant of Arabidopsis</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="10526529" href="https://independent.academia.edu/SilviaRegina1">Silvia 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href="https://unifr.academia.edu/ABuchala">Antony Buchala</a></div><p class="ds-related-work--metadata ds2-5-body-xs">PLANT PHYSIOLOGY, 2004</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Gene-Specific Involvement of  ß-Oxidation in Wound-Activated Responses in Arabidopsis&quot;,&quot;attachmentId&quot;:46454226,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/26104095/Gene_Specific_Involvement_of_%C3%9F_Oxidation_in_Wound_Activated_Responses_in_Arabidopsis&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/26104095/Gene_Specific_Involvement_of_%C3%9F_Oxidation_in_Wound_Activated_Responses_in_Arabidopsis"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="2" data-entity-id="18510849" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/18510849/A_gain_of_function_allele_of_TPC1_activates_oxylipin_biogenesis_after_leaf_wounding_in_Arabidopsis">A gain-of-function allele of TPC1 activates oxylipin biogenesis after leaf wounding in Arabidopsis</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="38539348" href="https://independent.academia.edu/EnricoMartinoia">Enrico 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href="https://www.academia.edu/18510849/A_gain_of_function_allele_of_TPC1_activates_oxylipin_biogenesis_after_leaf_wounding_in_Arabidopsis"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="3" data-entity-id="77213782" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/77213782/Homologs_of_old_yellow_enzyme_in_plants">Homologs of old yellow enzyme in plants</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="422585" href="https://al-balqa.academia.edu/SaeidAbuRomman">Saeid Abu-Romman</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Australian Journal of Crop Science, 2016</p><div 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