Amplicon Sequencing | GENEWIZ from Azenta

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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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mixed populations, and analysis multiple samples at scale.</p> <p>We offer various solutions for ultra-deep amplicon sequencing of PCR products to validate and discover variations in specific genomic regions or screen clones. Our amplicon sequencing services employ the latest technologies for custom, in-depth analysis of amplicons ranging from 100 base pairs (bp) to >10,000 bp.</p> <p>Applications for amplicon sequencing include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16S and 18S amplicon sequencing, screening of molecular clones, and analysis of plasmid stocks.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry?utm_source=amplicon-seq" target="_blank"><button>Request Quote</button></a></div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.azenta.com/amp-ez-sample?utm_campaign=2022-05 GEN NGS - Amplicon-EZ&utm_source=amplicon seq&utm_medium=right web ad&utm_content=US" target="_blank"><img alt="" src="/-/media/13005-D_Amplicon-EZ_CLIMS-Ad-US.ashx?la=en&hash=7A16A41F2912ACB998F6B466A7F6F57AEB7A3313" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> a {text-decoration: none; cursor: pointer;} .title {text-align: center;} .services-main { text-align: center; width: 100%; margin: 0 auto; } .services-main h2 { border: none; font-size: 18px; } .services-main h3 { font-size: 14px; margin: 0; } .service { display: inline-block; width: 25%; margin: 0 auto; padding: 10px 40px; vertical-align: top; } .table { margin-top: 20px; } .table>tbody>tr>td { vertical-align: middle; } button { border: none; background: #826ec3; color: #fff; border-radius: 3px; padding: 5px 20px; cursor: pointer; letter-spacing: 0.6px; } button:focus { outline: 0; } @media only screen and (max-width: 768px) { .services-main { width: 75%; margin: 0 auto; } .service { width: 100%; padding: 15px 0; } } </style> <div class="container title"> <h2>Amplicon Sequencing</h2> <p style="font-size: 16px;">Multiple Solutions to Answer Every Biological Question</p> </div> <div class="services-main"> <div class="service"> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Amplicon-EZ"></a> <h2>Amplicon-EZ</h2> <h3>Illumina<sup>®</sup>-based</h3> <p>50K reads/sample. Available for amplicons 150-500 bp in size.</p> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Amplicon-EZ"><button>Learn More</button></a> </div> <div class="service"> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Custom-Short-Read-Amplicon-Sequencing"></a> <h2>Custom Short-Read Amplicon Sequencing</h2> <h3>Illumina-based</h3> <p>Flexible data quantity options. Available for amplicons >100 bp.</p> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Custom-Short-Read-Amplicon-Sequencing"><button>Learn More</button></a> </div> <div class="service"> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Long-Read-Amplicon-Sequencing"></a> <h2>Long-Read Amplicon Sequencing</h2> <h3>PacBio<sup>®</sup> Sequel<sup>®</sup></h3> <p>Contiguous sequencing of amplicons up to 10 kb.</p> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Long-Read-Amplicon-Sequencing"><button>Learn More</button></a> </div> </div> <div class="table"> <div class="col-md-9 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td> </td> <td> </td> <td colspan="3">Azenta's Recommendation</td> </tr> <tr class="panel-heading"> <td> Amplicon Size</td> <td>Applications</td> <td colspan="2">Library Preparation Type</td> <td>Platform Configuration </td> </tr> <tr> <td>100-250 bp</td> <td style="text-align: left;"> <ul> <li>SNP Genotyping</li> <li>Genome editing clone verification</li> <li>Somatic/complex variant discovery</li> </ul> </td> <td class="text-left" style="text-align: center; vertical-align: middle;" colspan="2"> <p>Adapter ligation to<br /> full-length products</p> </td> <td class="text-left" style="text-align: center; vertical-align: middle;">Illumina 2x150 bp</td> </tr> <tr> <td rowspan="2"> 250-550 bp</td> <td> <ul> <li style="text-align: left;">Antibody heavy or light chain</li> <li style="text-align: left;"><span style="text-align: center;">Hypervariable 16S</span></li> </ul> </td> <td class="text-left" style="text-align: center; vertical-align: middle;" colspan="2"><span style="text-align: center;">Adapter ligation to</span><br style="text-align: center;" /> <span style="text-align: center;">full-length products</span></td> <td class="text-left" style="text-align: center; vertical-align: middle;">Illumina 2x250 bp<br /> or<br /> 2x300 bp</td> </tr> <tr> <td> <ul> <li style="text-align: left;">Somatic variant analysis </li> </ul> </td> <td class="text-left" style="text-align: center; vertical-align: middle;" colspan="2">Fragmentation</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Illumina 2x150 bp</td> </tr> <tr> <td rowspan="2">>550 bp</td> <td> <ul> <li style="text-align: left;">Paired antibody heavy and light chains</li> <li style="text-align: left;">Full length 16S</li> <li style="text-align: left;">Variant phasing</li> </ul> </td> <td class="text-left" style="text-align: center; vertical-align: middle;" colspan="2"><span style="text-align: center;">Adapter ligation to</span><br style="text-align: center;" /> <span style="text-align: center;">full-length products</span></td> <td class="text-left" style="text-align: center; vertical-align: middle;">PacBio Sequel</td> </tr> <tr> <td style="text-align: left;"> <ul> <li> Somatic variant analysis</li> </ul> </td> <td class="text-left" style="text-align: center; vertical-align: middle;" colspan="2">Fragmentation</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Illumina 2x150 bp</td> </tr> </tbody> </table> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is amplicon sequencing?</h2> <p>Amplicon sequencing is a targeted sequencing method that uses polymerase chain reaction (PCR) to selectively amplify specific DNA fragments or genomic regions of interest. Specific primers are designed to flank the target region and the resulting amplicons are then sequenced to determine the nucleotide sequence.</p> <p> </p> <h2> What is the difference between targeted sequencing and amplicon sequencing?</h2> <p>Targeted sequencing and amplicon sequencing both selectively sequence specific regions of the genome, rather than the whole genome. However, the difference between the two methods lies in how the regions of interest are selected and amplified.</p> <p>Targeted sequencing amplifies through hybridization capture and uses probes or baits complimentary to the target region in order to selectively capture and enrich for specific genomic regions, such as genes, exons, and other functional regions of the genome. Targeted sequencing often offers greater coverage than amplicon sequencing, as the use of hybridization capture can help mitigate biases in the representation of certain genomic regions.</p> <p>Amplicon sequencing amplifies DNA fragments using PCR. The target regions are defined by PCR primers designed to anneal specific sequences that flank the areas of interest, initiating the amplification process of the targeted DNA fragments. Amplicon sequencing is often more cost-effective than targeted sequencing as it does not involve the use of capture reagents.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11038-D_PEZ-DirectColonySeq_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 15px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; 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Read the case study to learn how this company was able to streamline clinical testing and accelerate the timeline of its phase 1/2 study. <br /> <a href="https://hubs.ly/Q01V2TnL0" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/MG_qPCR_Blog_US_Web-Tech-Thumb_Blue.ashx?la=en&hash=2A4957D1C2685CDC902644346D94E592804711C4" /> </div> <div class="resource resource-right"> <h3>Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide</h3> <p>This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making. <br /> <a href="https://blog.genewiz.com/ngs-qpcr-or-sanger-sequencing-an-assay-selection-guide?hs_preview=oBDChQvP-61477530694&utm_campaign=2022-03 GEN MG-qPCR&utm_source=Amplicon Sequencing&utm_medium=technical resources&utm_content=US" target="_blank"> <button>Read Blog</button> </a> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Sample Submission Guidelines</h2> <p>Azenta accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our <a href="/en/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines" style="color: #826ec3; text-decoration: underline;">Sample Submission Guidelines</a>. </p> <p> </p> <h2>Deliverables</h2> All customers receive their raw data as FASTQ files. Customizable data analysis packages are available by request. </div> <br> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Illumina"><img alt="Illumina" src="/-/media/hires-illumina-logo-rgb_new.ashx?w=200&la=en&hash=2DE7778E5877DA9F3D063A9FAF021DA575ECF223" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#PacBio"><img alt="PacBio" src="/-/media/PacBio-CSP_logo.ashx?w=200&la=en&hash=1568DC13B370C032463997ED7BC5591C1FECF672" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a 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