Organoids And 3D Models - Cancer Science

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These <a href="https://cancerscience.net/about/index/miniature-organ-like-structures" title="miniature organ-like structures" target="_blank">miniature organ-like structures</a> provide a more physiologically relevant model than traditional 2D cell cultures, making them invaluable in cancer research.</div><div><a href="https://cancerscience.net/knowledgebase/how-are-organoids-created" title="How are Organoids Created?"><h3>How are Organoids Created?</h3></a>The creation of organoids involves isolating stem cells or progenitor cells from tissues, which are then embedded in an extracellular matrix and cultured in a medium containing the necessary growth factors. Over time, these cells self-organize into 3D structures that resemble the original organ from which the cells were derived.</div><div><a href="https://cancerscience.net/knowledgebase/why-are-organoids-important-in-cancer-research" title="Why are Organoids Important in Cancer Research?"><h3>Why are Organoids Important in Cancer Research?</h3></a>Organoids are crucial for cancer research for several reasons:</div><div> <a href="https://cancerscience.net/about/index/mimic-tumor-microenvironment" title="Mimic Tumor Microenvironment" target="_blank">Mimic Tumor Microenvironment</a>: They offer a realistic tumor microenvironment, which is essential for studying cancer progression and <a href="https://cancerscience.net/about/index/drug-response" title="drug response" target="_blank">drug response</a>.<br> <a href="https://cancerscience.net/about/index/patient-specific-models" title="Patient-Specific Models" target="_blank">Patient-Specific Models</a>: Organoids can be derived from patient-specific tissues, allowing for personalized medicine approaches.<br> <a href="https://cancerscience.net/about/index/high-throughput-screening" title="High-Throughput Screening" target="_blank">High-Throughput Screening</a>: They enable high-throughput screening of therapeutic compounds, expediting the discovery of effective treatments.</div><div><a href="https://cancerscience.net/knowledgebase/what-are-3d-cancer-models" title="What are 3D Cancer Models?"><h3>What are 3D Cancer Models?</h3></a>3D cancer models are advanced in vitro systems that replicate the complexity of tumor tissues more accurately than traditional 2D cultures. These models include <a href="https://cancerscience.net/about/index/spheroids" title="spheroids" target="_blank">spheroids</a>, <a href="https://cancerscience.net/about/index/scaffolds" title="scaffolds" target="_blank">scaffolds</a>, and bioprinted tissues, providing a more accurate representation of tumor biology.</div><div><a href="https://cancerscience.net/knowledgebase/how-are-3d-models-different-from-2d-cultures" title="How are 3D Models Different from 2D Cultures?"><h3>How are 3D Models Different from 2D Cultures?</h3></a>3D models differ from 2D cultures in several ways:</div><div> <a href="https://cancerscience.net/about/index/spatial-organization" title="Spatial Organization" target="_blank">Spatial Organization</a>: 3D models maintain the spatial organization of cells, similar to in vivo conditions, whereas 2D cultures are flat and lack this complexity.<br> <a href="https://cancerscience.net/about/index/cell-cell-interactions" title="Cell-Cell Interactions" target="_blank">Cell-Cell Interactions</a>: They allow for more realistic cell-cell and cell-matrix interactions, which are critical for studying cancer behavior.<br> <a href="https://cancerscience.net/about/index/drug-penetration" title="Drug Penetration" target="_blank">Drug Penetration</a>: 3D models better replicate the challenges of drug penetration and distribution seen in real tumors.</div><div><a href="https://cancerscience.net/knowledgebase/what-are-the-applications-of-organoids-and-3d-models-in-cancer" title="What are the Applications of Organoids and 3D Models in Cancer?"><h3>What are the Applications of Organoids and 3D Models in Cancer?</h3></a>Organoids and 3D models have diverse applications in cancer research, including:</div><div> <a href="https://cancerscience.net/about/index/drug-screening" title="Drug Screening" target="_blank">Drug Screening</a>: They are used for screening and identifying potential anticancer drugs.<br> <a href="https://cancerscience.net/about/index/studying-metastasis" title="Studying Metastasis" target="_blank">Studying Metastasis</a>: Researchers use them to study the mechanisms of metastasis and invasion.<br> <a href="https://cancerscience.net/about/index/genetic-studies" title="Genetic Studies" target="_blank">Genetic Studies</a>: They are ideal for studying the genetic mutations involved in cancer.<br> <a href="https://cancerscience.net/about/index/immunotherapy-research" title="Immunotherapy Research" target="_blank">Immunotherapy Research</a>: These models are used to evaluate the efficacy of immunotherapies.</div><div><a href="https://cancerscience.net/knowledgebase/what-are-the-limitations-of-organoids-and-3d-models" title="What are the Limitations of Organoids and 3D Models?"><h3>What are the Limitations of Organoids and 3D Models?</h3></a>Despite their advantages, organoids and 3D models have limitations:</div><div> <a href="https://cancerscience.net/about/index/complexity" title="Complexity" target="_blank">Complexity</a>: The complexity of creating and maintaining these models can be a barrier.<br> <a href="https://cancerscience.net/about/index/standardization" title="Standardization" target="_blank">Standardization</a>: There is a lack of standardization in protocols, making reproducibility a challenge.<br> <a href="https://cancerscience.net/about/index/scale" title="Scale" target="_blank">Scale</a>: Scaling up these models for high-throughput applications can be difficult.</div><div><h3>Future Prospects</h3>The future of cancer research with organoids and 3D models is promising. Advances in <a href="https://cancerscience.net/about/index/bioprinting" title="bioprinting" target="_blank">bioprinting</a>, <a href="https://cancerscience.net/about/index/microfluidics" title="microfluidics" target="_blank">microfluidics</a>, and <a href="https://cancerscience.net/about/index/genome-editing" title="genome editing" target="_blank">genome editing</a> are expected to overcome current limitations and open new avenues for personalized medicine and targeted therapies. Researchers are optimistic that these technologies will revolutionize our understanding and treatment of cancer in the coming years.</div></div> </div> <div id="recent_papers"> <br><hr /><br><h2 class="heading1">Relevant Publications</h2> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/39192131" target='_blank' title="The Application of Organoids in the Study of Antiviral Innate Immunity.">The Application of Organoids in the Study of Antiviral Innate Immunity.</a> </div> <p><strong>Issue Release:</strong> 2025</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38947380" target='_blank' title="Tanreqing injection inhibits stemness and enhances sensitivity of non-small cell lung cancer models to gefitinib through ROS/STAT3 signaling pathway.">Tanreqing injection inhibits stemness and enhances sensitivity of non-small cell lung cancer models to gefitinib through ROS/STAT3 signaling pathway.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38866213" target='_blank' title="Myoferlin: a potential marker of response to radiotherapy and survival in locally advanced rectal cancer.">Myoferlin: a potential marker of response to radiotherapy and survival in locally advanced rectal cancer.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38940869" target='_blank' title="Clinicopathological Factors as Predictors for Establishment of Patient Derived Head and Neck Squamous Cell Carcinoma Organoids.">Clinicopathological Factors as Predictors for Establishment of Patient Derived Head and Neck Squamous Cell Carcinoma Organoids.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38928228" target='_blank' title="Human Brain In Vitro Model for Pathogen Infection-Related Neurodegeneration Study.">Human Brain In Vitro Model for Pathogen Infection-Related Neurodegeneration Study.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/39097123" target='_blank' title="Extracellular matrix regulation of cell spheroid invasion in a 3D bioprinted solid tumor-on-a-chip.">Extracellular matrix regulation of cell spheroid invasion in a 3D bioprinted solid tumor-on-a-chip.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/39107846" target='_blank' title="Research progress of brain organoids in the field of diabetes.">Research progress of brain organoids in the field of diabetes.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38914075" target='_blank' title="3D embedded bioprinting of large-scale intestine with complex structural organization and blood capillaries.">3D embedded bioprinting of large-scale intestine with complex structural organization and blood capillaries.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38915614" target='_blank' title="Cellpose as a reliable method for single-cell segmentation of autofluorescence microscopy images.">Cellpose as a reliable method for single-cell segmentation of autofluorescence microscopy 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style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38817733" target='_blank' title="Hepatic spheroid-on-a-chip: Fabrication and characterization of a spheroid-based model of the human liver for drug screening applications.">Hepatic spheroid-on-a-chip: Fabrication and characterization of a spheroid-based model of the human liver for drug screening applications.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38778114" target='_blank' title="Acquisition of epithelial plasticity in human chronic liver disease.">Acquisition of epithelial plasticity in human chronic liver disease.</a> </div> <p><strong>Issue Release:</strong> 2024</p> </div> <div class='publication-block'> <div style='margin-bottom: 10px;line-height: 24px;'> <a href="https://pubmed.ncbi.nlm.nih.gov/38774450" target='_blank' title="Establishment and 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