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li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}body.header-shop #Top_bar #menu{display:flex!important;background-color:transparent}.header-shop #Top_bar.is-sticky .top_bar_row_second{display:none}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;transform:unset}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:not(.menu-toggle):after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span{border-width:0!important}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}.menuo-no-borders.header-plain #Top_bar a#header_cart,.menuo-no-borders.header-plain #Top_bar a#search_button,.menuo-no-borders.header-plain #Top_bar .wpml-languages,.menuo-no-borders.header-plain #Top_bar a.action_button{border-width:0}.menuo-right #Top_bar .menu_wrapper{float:right}.menuo-right.header-stack:not(.header-center) #Top_bar .menu_wrapper{margin-right:150px}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank,body.under-construction.header-rtl.header-creative.header-open{padding-left:0!important;padding-right:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background-color:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar #menu{background-color:transparent}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{margin:0;width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}.menuo-arrows #Top_bar .menu > li.submenu > a > span:after{content:unset!important}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:left;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{content:none}#Header_creative #Top_bar .top_bar_right .top_bar_right_wrapper{flex-wrap:wrap;justify-content:center}#Header_creative #Top_bar .top_bar_right .top-bar-right-icon,#Header_creative #Top_bar .top_bar_right .wpml-languages,#Header_creative #Top_bar .top_bar_right .top-bar-right-button,#Header_creative #Top_bar .top_bar_right .top-bar-right-input{min-height:30px;margin:5px}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .contact_details{width:100%;text-align:center;margin-bottom:20px}#Header_creative #Action_bar .contact_details li{padding:0}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative #Action_bar .social-menu{float:none;text-align:center}#Header_creative #Action_bar .social-menu li{border-color:rgba(0,0,0,.1)}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}.header-open #Header_creative #Action_bar{display:block}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left;width:100%}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;z-index:203}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul.sub-menu li:not(:last-of-type) a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1240px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none;box-shadow:unset}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo,#Top_bar.is-sticky .custom-logo-link{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}.logo-sticky-width-auto #Top_bar.is-sticky #logo img.logo-sticky{width:auto}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky,.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li:not(.current-menu-item) > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right .top-bar-right-icon{color:rgba(255,255,255,.9)}.sticky-dark #Top_bar.is-sticky .top_bar_right .top-bar-right-icon svg .path{stroke:rgba(255,255,255,.9)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.1);border-color:rgba(0,0,0,0.1)}.sticky-white #Top_bar.is-sticky,.sticky-white #Top_bar.is-sticky #menu{background:rgba(255,255,255,.8)}.sticky-white #Top_bar.is-sticky .menu > li:not(.current-menu-item) > a{color:#222}.sticky-white #Top_bar.is-sticky .top_bar_right .top-bar-right-icon{color:rgba(0,0,0,.8)}.sticky-white #Top_bar.is-sticky .top_bar_right .top-bar-right-icon svg .path{stroke:rgba(0,0,0,.8)}.sticky-white #Top_bar.is-sticky .wpml-languages a.active,.sticky-white #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(255,255,255,0.1);border-color:rgba(0,0,0,0.1)}}@media only screen and 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#Top_bar:not(.is-sticky){background-color:rgba(255,255,255,0.8)}}@media only screen and ( max-width:767px ){#Top_bar{background-color:#ffffff !important}#Action_bar{background-color:#007dc5 !important}#Action_bar .contact_details{color:#222222}#Action_bar .contact_details a{color:#0095eb}#Action_bar .contact_details a:hover{color:#007cc3}#Action_bar .social li a,#Action_bar .social-menu a{color:#bbbbbb!important}#Action_bar .social li a:hover,#Action_bar .social-menu a:hover{color:#777777!important}} form input.display-none{display:none!important} </style> <link rel='stylesheet' id='style-css' href='https://www.wyatt.com/wp-content/themes/betheme-child/style.css?ver=6.4.5' type='text/css' media='all' /> <style id='mfn-custom-inline-css' type='text/css'> @supports (-ms-ime-align: auto) { } span {font-family:"Open Sans", Arial, Tahoma, sans-serif !important;} @media only screen and (min-width: 1240px) { .header-classic #Header .top_bar_left {width:1306px;} #Top_bar .menu > li > a 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text-transform:unset;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover span {text-decoration:none ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu ul {background:transparent; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a {color:#007dc5; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a:hover {background:transparent;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a {padding:5px 0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a:hover {text-decoration:underline;} .mm-vertical #Top_bar .menu > li > ul {padding:20px 20px 20px 2px;} .mm-vertical #Top_bar .menu > li > ul a {padding:5px; border:0px; color:#007dc5;} .mm-vertical #Top_bar .menu > li > ul a:hover {background:transparent; } .mm-vertical #Top_bar .menu > li > ul a:hover span {text-decoration:underline ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li {padding-top:20px; padding-bottom:25px; border-right: 0px solid rgba(0, 0, 0, 0.05);} /*for parent title in sbmenu*/ .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 {padding-top:20px; line-height:30px; border-bottom:1px solid #ccc; font-weight:600;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 a{color:#444444;} /*for contact submenu*/ .mm-vertical #Top_bar .menu > li#menu-item-2437 li.mfn-megamenu-cols-2 li {} /*menu button css*/ .mm-vertical #Top_bar .menu > li#menu-item-5221 a span {background:#007dc5; color:#fff; border-radius:6px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-5221 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-5221 > a, #Top_bar.is-sticky .menu > li#menu-item-5221 > a span {line-height:30px; margin-top:0px;} .mm-vertical #Top_bar li#menu-item-6152 a {color:#000 !important; padding-top:0px !important; white-space:nowrap; color: #007dc5 !important; font-weight: 400;} /*set width of menu item*/ .mm-vertical #Top_bar li#menu-item-2341 {width:343px; padding-left: 80px;} .mm-vertical #Top_bar li#menu-item-2348 {width:278px;} .mm-vertical #Top_bar li#menu-item-2355 {width:288px;} .mm-vertical #Top_bar li#menu-item-2363 {width:240px;} .mm-vertical #Top_bar li#menu-item-2369 {width:541px; padding-left:70px;} .mm-vertical #Top_bar li#menu-item-2379 {width:340px;} .mm-vertical #Top_bar li#menu-item-2384 {width:280px;} .mm-vertical #Top_bar li#menu-item-2391 {width:350px; padding-left:80px;} .mm-vertical #Top_bar li#menu-item-2395 {width:310px;} .mm-vertical #Top_bar li#menu-item-2403 {width:310px;} .mm-vertical #Top_bar li#menu-item-2408 {width:210px;} .mm-vertical #Top_bar li#menu-item-5918 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5919 {width:387px;} .mm-vertical #Top_bar li#menu-item-5920 {width:280px;} .mm-vertical #Top_bar li#menu-item-2427 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5235 {width:397px;} .mm-vertical #Top_bar li#menu-item-5765 {width:280px;} .mm-vertical #Top_bar li#menu-item-5235 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5765 {width:397px;} .mm-vertical #Top_bar li#menu-item-15082 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-15044 {width:397px;} } @media only screen and (max-width: 1239px) { #menu > ul > li > a, a.action_button, #overlay-menu ul li a { font-size: 15px; font-weight: 400; letter-spacing: 1px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar #menu ul li ul li a {font-size:16px;} .mm-vertical #Top_bar #menu ul li ul li ul li a {font-size:15px;} } /* Header */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1;} .header-classic:not(.home) #Content { border-top: 0px solid #e1e1e1; } .header-classic.home .mfn-rev-slider { border-top: 1px solid #e1e1e1; } /*Action Bar*/ #Action_bar a {color:#fff;} /* Widget */ .widget-area:before { display: none; } .aside_left .widget-area { border: 0; } /* Icon box */ .icon_box.has_border { background: #fff; border: 1px solid #e1e1e1; padding: 40px 10%; } .icon_box.no_border {background: #fff; border:0px; padding: 40px 10%;} .icon_box.has_border:after {border:0px} /* Offer box */ .offer_box { background-color: #252525; padding: 20px 20px 5px; text-align: center; } .offer_box .offer_box_list { border-width: 1px 1px 0 1px; border-style: solid; border-color: #929292; font-weight: 700; font-size: 16px; letter-spacing: 2px; margin-bottom: 20px; } .offer_box .offer_box_list a { display: block; color: #fff; border-bottom: 1px solid #929292; padding: 18px 15px; word-wrap: break-word; } /* Footer */ /*#Footer { border-top: 1px solid #e1e1e1; }*/ #Footer .widget > h4 { font-size: 18px; font-weight: 300; margin-bottom: 20px; } #Footer .footer_copy { background: #465158; border-top: 0; } .before-footer {background: #f9f9f9;} #Footer .footer_copy .social { display: none; } /**/ .top-widgets-container {float:right} .top-widgets-container .widget {padding-bottom:0px; margin-bottom:0px; float:right;margin-top:0px;} .top-widgets-container .widget ul.menu{list-style:none; padding:0px;} .top-widgets-container .widget ul.menu li {float:left; padding:12px 0px;} .top-widgets-container .widget ul.menu li a{padding:0px 20px; border-right:1px solid #ccc; line-height:15px;} .top-widgets-container .widget ul.menu li:last-child a{border-right:0px;} .top-widgets-container .widget_search .icon-search-fine {font-size:24px; line-height:40px;} .top-widgets-container .widget_search input[type="text"] {width:82%; float:right; padding:5px 10px 5px 5px; border:0px; margin-top:7px; height:30px;min-height:30px;} #menu-top-navigation .cart a {padding:0px 20px; } #menu-top-navigation .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} .mobilecart {display:none; width:30px; float:right; margin-top:11px;} .mobilecart a { padding:0px; border:0px;} .mobilecart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; left:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { .top-widgets-container .widget {float:none;} .top-widgets-container .widget.widget_nav_menu {max-width:245px; margin:0px auto; clear:both;} .top-widgets-container .widget ul.menu li a {padding:0px 15px;} #menu-top-navigation .cart.menu-item-2449 {display:none;} #menu-top-navigation .cart a {padding:0px 10px;} .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .widget:after {display:none;} .top-widgets-container {float:none; margin:0px auto;} .top-widgets-container .widget_search input[type="text"] {width:80%;} .mobilecart {display:block;} .top-widgets-container .widget_search {width:254px; overflow:hidden;padding-bottom:20px; margin:0px auto;} .top-widgets-container .widget_search .searchform{width:210px; float:left;} } .icon-100 .image_wrapper img {width:150px; height:150px;} .down-heading, .heading {padding-left:17px;} @media only screen and (min-width:320px) and (max-width:767px) { .down-heading, .heading {padding:0px; text-align:center;} } .ui-tabs .ui-tabs-nav li {border-width:0px;} .page-id-2602 .ui-tabs .ui-tabs-nav li a {padding:14px 28px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li {border:0px; padding:5px 0px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {background-image:none; font-size:100%; color:#2991d6; padding:5px 10px 5px 20px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a:hover {text-decoration:underline;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a {color:#000; border-left:3px solid #2991d6; border-radius:0px !important; padding:5px 10px 5px 17px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:23%; border:0px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:76.7%; border:0px !important; padding-top:5px;} @media only screen and (min-width:768px) and (max-width:1239px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {font-size:100%;} } @media only screen and (min-width:320px) and (max-width:767px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:100%;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:100%; padding-top:15px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {-webkit-box-shadow:none;-moz-box-shadow:none;box-shadow:none;} } .ui-tabs .ui-tabs-nav li h3 {margin:0px; padding:0px;} .ui-tabs .ui-tabs-nav li h3 a { text-decoration: none; display: block; padding: 14px 30px !important; font-size: 100%; font-weight:initial; } .ui-tabs .ui-tabs-panel .icon_box {padding-bottom:2px;} .ui-tabs .ui-tabs-panel .column.one-fourth {width:25%;} .ui-tabs .ui-tabs-panel .column.one-second, .ui-tabs .ui-tabs-panel .column.one-fourth, .ui-tabs .ui-tabs-panel .column.one-third, .ui-tabs .ui-tabs-panel .column.two-third{margin-left:0px; margin-right:0px;} @media only screen and (min-width: 960px) and (max-width: 1239px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:10px 20px !important;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:5px 10px !important;} } /* Make Nav Subheadings unclickable */ .unclickable > a:hover { cursor: default;} .wp-caption-text { background: transparent; color: #075c97; font-size:90%;} .ls-wrapper h3 a {color: #075c97;} .column_sidebar_widget {float:none;} /*app notes*/ ul.clients.appnotes .client_wrapper {text-align:left; height:auto !important; line-height:auto !important; overflow:hidden;} ul.clients.appnotes li:nth-child(2n+1) {clear:both;} ul.clients.appnotes .client_wrapper img { position:relative; left:auto !important; top: auto !important; float:left; -webkit-transform: none!important; -moz-transform: none!important; transform: none!important; padding-right:20px; width:auto!important; } ul.clients.appnotes .client_wrapper h3 { line-height:20px; font-size:16px; letter-spacing:1px; padding-top:10px;} ul.clients.appnotes .client_wrapper a:hover {text-decoration:none;} @media only screen and (max-width: 640px) { ul.clients.appnotes li {width:100% !important; clear:both;} } .pager {padding-top:20px;} .pager .page-numbers {border:1px solid #ccc; padding:3px 5px;} .pager .prev.page-numbers, .pager .next.page-numbers {border:0px;} .alignleft {margin:3px 10px 10px 0px;} dl {clear:both;} .team.team_vertical img.scale-with-grid {width:100%;} .column_placeholder {margin-bottom:0px;} .icon-height img{max-height:50px;} .single .post-header {margin-left:0px; margin-right:0px;} .single .section-post-header .single-photo-wrapper.image {display:none;} .widget_mfn_menu ul li.menu-item-has-children > a::after {content: '\e868'; font-family: "mfn-icons"; right:2px; position:absolute; width:20px; height:15px; color:#ccc; } table th ul, table td ul {list-style-position:inside !important;} .sticky-white #Top_bar.is-sticky, .sticky-white #Top_bar.is-sticky #menu, #Top_bar.is-sticky {background:#fff; opacity:1;} /*Webinars latest posts*/ .parent-pageid-3663 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-3663 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-3663 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-3663 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .parent-pageid-3663 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-3663 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars*/ .page-id-2653 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-2653 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-2653 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-2653 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-2653 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .page-id-2653 .Latest_news ul li .desc_footer .date {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-2653 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-2653 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-2653 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Newsletter Archive*/ .page-id-3661 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-3661 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-3661 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-3661 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-3661 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li.category-newsletter-archive .desc h5 a{color:#007dc5;} .page-id-3661 .Latest_news ul li .desc_footer .date {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-3661 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-3661 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-3661 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Ask the Expert*/ .page-id-5348 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-5348 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-5348 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-5348 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc h5{margin-bottom:0px;} .page-id-5348 .Latest_news ul li.category-ask-the-expert .desc h5 a{color:#007dc5;} .page-id-5348 .Latest_news ul li .desc_footer {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .date {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-5348 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-5348 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-5348 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; font-weight:normal; line-height:20px;} /*accordion title as h3*/ .accordion .question .title h3 {margin-bottom:0px; } .accordion .question.active .title h3{color:#2991d6} /*column padding*/ .column, .columns {margin:0px 10px 40px;} .accordion .answer .one-second.column {width:47%;} .wrap .one-second.column {width:47%;} /*.column_column ul li {margin-bottom:2px;}*/ .single .post-header {margin-bottom:10px;} /* Appnote seach box*/ .widget_appnote_search_widget {clear:both; display:block; overflow:hidden;} .widget_appnote_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_appnote_search_widget .searchform {display:inline-block; float:left;} .widget_appnote_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_appnote_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_appnote_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_appnote_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*flat box*/ .flat_box .photo_wrapper .icon {display:none;} .flat_box .photo_wrapper img.photo {transform: translateX(0px)} .flat_box .desc_wrapper {margin-left:0px;} /*center images at user profile*/ .page-id-2504 .image_frame .image_wrapper {text-align:center} .page-id-2504.if-overlay .image_frame:hover .image_wrapper img, .page-id-2504.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .if-overlay .image_frame .image_wrapper .image_links {display:none;} /* Changes for ClickDimensions submit button */ a.button_theme, a.tp-button.button_theme, button, input[type="submit"], input[type="reset"], input[type="button"] { background-color: #0065a4 !important; color: #ffffff !important; font-family: inherit !important; /*padding:0px;*/ } /*isotope wrapper*/ .blog_wrapper.isotope_wrapper .post-item .post-footer {background:none;} .blog_wrapper.isotope_wrapper .post-item .post-footer .post-links{border:0px;} .blog_wrapper.isotope_wrapper .grid .post-photo-wrapper {border:0px;} /* product page*/ .page-id-2582 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-2582 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-2582 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .uk-margin-small-right {vertical-align:middle; margin-top:-2px;} .page-id-2647 .ui-tabs .ui-tabs-panel .column.one-second {margin-bottom:0px;} .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {border-color:transparent;} @media only screen and (min-width: 960px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {width:12%;} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:88%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper { width:25%} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:75%;} } @media only screen and (min-width: 768px) { .page-id-2640 .column.column_pricing_item {margin:0px 1% 40px;} } /* contact form 7 submit button*/ .wpcf7-form-control.wpcf7-submit {padding:10px 30px;} .wpcf7 .red {color:#990000;} .wpcf7 span.wpcf7-list-item {display:block; margin:0px;} .wpcf7 label {font-weight:400;} #lostusername {border:1px solid #ddd; padding:30px 20px 0px;} #lostusername .input {width:100%;} #lostusername .button {padding:10px 20px;} .ur-frontend-form button[type="submit"], .ur-frontend-form input[type="submit"] {float:none;} .page-id-2690.if-overlay .image_frame:hover .image_wrapper img, .page-id-2690.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-2690 .team .desc_wrapper hr {display:none;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-2690 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* webinars seach box*/ .widget_webinars_search_widget {clear:both; display:block; overflow:hidden;} .widget_webinars_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_webinars_search_widget .searchform {display:inline-block; float:left;} .widget_webinars_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_webinars_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_webinars_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_webinars_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*Recent Webinars nav issue fix*/ .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a:after {display:none;} /*login-in nav issue fix*/ .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* Contact Customer Support nav issue fix*/ .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* custom seach box*/ .search #Content .searchform {display:inline-block; float:left;} .search #Content .searchform h4{clear:both; padding:10px 0px; margin:0px;} .search #Content .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .search #Content .searchform select {-webkit-appearance:menulist; padding:7px 10px;} .search #Content .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .search #Content .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .search #Content .searchform input[type=submit] {margin:0px; margin-top:15px;} } .wpcf7-form .anr_captcha_field {position:absolute;} /*newsletter archive tables remove border*/ .post.category-newsletter-archive .post-wrapper-content table td {box-shadow:none; border:0px;} .be-ix-link-block {max-width:750px; text-align:center; margin:0px auto; padding-top:20px;} #Footer .widgets_wrapper .column {margin:0px 9px 0px;} @media only screen and (min-width: 768px) and (max-width: 959px) { #Footer .widgets_wrapper .column {margin:0px 5px 0px;} } /*Login page popup */ .page-id-5451.logged-in #pum-8728 {display:none !important;} #cookie-law-info-bar a {color:#fff; text-decoration:underline;} #cookie-law-info-bar a:hover {text-decoration:none;} #cookie-law-info-bar .cli-plugin-button {text-decoration:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { #cookie-law-info-again{display:none !important;} } /*After Multi Language SETUP*/ #Action_bar .column {overflow:visible;} .top-widgets-container .widget_search input[type="text"] {margin-top:5px;} .widget.widget_nav_menu::after, .widget.widget_icl_lang_sel_widget::after, .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .wpml-ls-legacy-dropdown-click {margin-top:6px; } .wpml-ls-legacy-dropdown-click a, .wpml-ls-legacy-dropdown-click a:hover, .wpml-ls-legacy-dropdown-click a:focus, .wpml-ls-legacy-dropdown-click .wpml-ls-current-language:hover>a {color:#626262 !important; border:0px;text-decoration:none;} .wpml-ls-legacy-dropdown-click .wpml-ls-sub-menu {z-index:999;} .top-widgets-container h2.title {display:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { .wpml-ls-legacy-dropdown-click {margin:6px auto 0px; } .top-widgets-container .widget_search {clear:both;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .wpml-ls-legacy-dropdown-click {width:auto;} } #menu-top-navigation-ch .cart a {padding:0px 20px; } #menu-top-navigation-ch .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ch .cart.menu-item-2449 {display:none;} #menu-top-navigation-ch .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-13087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-13087 > a, #Top_bar.is-sticky .menu > li#menu-item-13087 > a span {line-height:30px; margin-top:0px;} } .post-9911 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .post-9911 span.wpcf7-form-control-wrap{margin-bottom:20px;} .post-9911 .wpcf7-list-item-label{color:#000000;} .post-9911 .unique .one-third.column{width:30.333% !important;} .post-9911 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .post-9911 .wrap .one-second.column{width:32%;} .post-9911 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .post-9911 .wpcf7 span.wpcf7-list-item{width: 100%;} .post-9911 .unique .one-third.column{width:100% !important;} .post-9911 .wrap .one-second.column{width:100%;} .post-9911 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*remove meta from search-results*/ .search.search-results .post-item .post-meta {display:none;} .search.search-results .post-item .post-desc {padding-left:0px;} /*footer update*/ /*#Footer .widgets_wrapper {padding-bottom:10px;} #Footer .widgets_wrapper .column {margin-bottom:10px;} #Footer #custom_html-4 > h4 {text-align:center;} #Footer #custom_html-14 > h4 {text-align:center;} #Footer #custom_html-9 {max-width:160px; margin:0px auto; clear:both; padding-top:25px;} .footer-resources {max-width:80%; margin:0px auto; } .footer-resources li {float:left; line-height:28px;} .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:12%; clear:both;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:28%;} html[lang="zh-hans"] .footer-resources li:nth-child(1), html[lang="zh-hans"] .footer-resources li:nth-child(6){width:15%; clear:both;} html[lang="zh-hans"] .footer-resources li:nth-child(2), html[lang="zh-hans"] .footer-resources li:nth-child(7){width:25%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:17%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:18%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:25%;} @media only screen and (min-width:320px) and (max-width:1239px) { .footer-resources {max-width:60%; } .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:50%; clear:none;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:50%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:50%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:50%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:50%;} } @media only screen and (min-width:320px) and (max-width:767px) { .footer-resources {max-width:100%; } #Footer #custom_html-4 > h4 {text-align:left;} #Footer #custom_html-14 > h4 {text-align:left;} #Footer #custom_html-9 {max-width:100%;} } */ .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display:inline-block; text-decoration: none; padding: 0px 20px !important; border:0px; text-transform: uppercase; line-height:30px; font-size: 15px; font-weight: 400;letter-spacing: 1px;} @media only screen and (min-width:320px) and (max-width:767px) { .tabs_vertical.ui-tabs .ui-tabs-nav li h3, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active h3 {text-align:center;} .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover {margin:20px auto;} } /*Webinars latest posts zh-hans*/ .parent-pageid-11725 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-11725 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-11725 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-11725 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .parent-pageid-11725 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-11725 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars zh-hans*/ .page-id-11764 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-11764 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-11764 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-11764 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-11764 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .page-id-11764 .Latest_news ul li .desc_footer .date {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-11764 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-11764 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-11764 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Request Product Quote zh-hans*/ .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12227 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12227 .wpcf7-list-item-label{color:#000000;} .page-id-12227 .unique .one-third.column{width:29% !important;} .page-id-12227 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12227 .wrap .one-second.column{width:32%;} .page-id-12227 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12227 .unique .one-third.column{width:100% !important;} .page-id-12227 .wrap .one-second.column{width:100%;} .page-id-12227 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Request Product Info zh-hans*/ .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12612 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12612 .wpcf7-list-item-label{color:#000000;} .page-id-12612 .unique .one-third.column{width:29% !important;} .page-id-12612 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12612 .wrap .one-second.column{width:32%;} .page-id-12612 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12612 .unique .one-third.column{width:100% !important;} .page-id-12612 .wrap .one-second.column{width:100%;} .page-id-12612 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Team in zh-hans*/ .page-id-12002.if-overlay .image_frame:hover .image_wrapper img, .page-id-12002.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-12002 .team .desc_wrapper hr {display:none;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-12002 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* product page in zh-hans*/ .page-id-12156 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-12156 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-12156 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } /*Recent Webinars nav issue fix for zh-hans*/ .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a:after {display:none;} .large h3{font-size:22px; line-height:30px;} .small {margin-left:25px;} sup {display:inline-block; margin-top:-4px;} sub {display:inline-block; margin-bottom:-4px;} sup, sub {display:inline-table;} .ui-tabs-panel .one-second.column {width:50%;} .parent-pageid-2582 .ui-tabs-panel td {text-align:left !important;} .ls-borderlessdark3d .ls-shadow { display: none !important;} #menu-top-navigation-de .cart a {padding:0px 20px;} #menu-top-navigation-de .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-de .cart.menu-item-14543{display:none;} #menu-top-navigation-de .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-15087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-15087 > a, #Top_bar.is-sticky .menu > li#menu-item-15087 > a span {line-height:30px; margin-top:0px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar li#menu-item-12984 a {color:#000 !important; padding-top:20px !important;} .mm-vertical #Top_bar li#menu-item-14978 a {color:#000 !important; padding-top:20px !important;} } #Footer #custom_html-15 > h4 {text-align:center;} /* product page German */ .page-id-14845 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-14845 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-14845.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-14295.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-14845 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .parent-pageid-14845 .ui-tabs-panel td {text-align:left !important;} .button_align.align_left .button {text-align:left; padding:10px 0;} .button_align.align_left .as_tab {font-size: 18px; line-height: 20px; font-weight: 400; background:#fff; letter-spacing: 0px; color: #2991d6 !important; padding: 5px 10px 5px 20px;margin:0; } .button_align.align_left .button.as_tab:hover {text-decoration:underline !important; background-color:#fff!important;} .button_align.align_left .button.as_tab:after {display:none;} .button_align.align_left .button.as_tab.active {color: #000; border-left: 3px solid #2991d6; border-radius: 0px !important; padding: 5px 10px 5px 17px !important;} .button_align.align_left .button.as_tab .button_label {margin:0!important;} .button_align.align_left .button.as_tab_btn { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display: inline-block; text-decoration: none; padding: 0px 20px !important; border: 0px; text-transform: uppercase; line-height: 30px; font-size: 15px; font-weight: 400; letter-spacing: 1px; } /*Japan language*/ #Footer #custom_html-22 > h4 { text-align: center; } #menu-top-navigation-ja .cart a {padding:0px 20px;} #menu-top-navigation-ja .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ja .cart.menu-item-2449 {display:none;} #menu-top-navigation-ja .cart a {padding:0px 10px;} } .mm-vertical #Top_bar .menu > li#menu-item-18403 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-18403 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-18403 > a, #Top_bar.is-sticky .menu > li#menu-item-18403 > a span {line-height:30px; margin-top:0px;} @media only screen and (min-width: 1240px) { #Top_bar #menu-german-main-menu > li > a span:not(.description) { padding: 0 3px; font-size:14px; } } body, ul.timeline_items, .icon_box a .desc, .icon_box a:hover .desc, .feature_list ul li a, .list_item a, .list_item a:hover, .widget_recent_entries ul li a, .flat_box a, .flat_box a:hover, .story_box .desc, .content_slider.carousel ul li a .title, .content_slider.flat.description ul li .desc, .content_slider.flat.description ul li a .desc, .post-nav.minimal a i { color: #333333 !important; } .column_column ul, .column_column ol, .the_content_wrapper:not(.is-elementor) ul, .the_content_wrapper:not(.is-elementor) ol { color: #333333 !important; } .hero { margin-top: 20px; } .bg-hero { background: radial-gradient(circle at 100% 0,#ebf1f2 0% 30%,#0e81a7 100%); } .hero-full { min-height: 450px; background-color: rgba(0, 0, 0, 0.5); } .column, .columns { margin: 0px 8px 40px; } .image-container { position: relative; display: inline-block; /* oder 'block' je nach Layout */ } .ribbon { position: absolute; top: 10px; /* Abstand von oben */ left: -20px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #FF5733; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 5px 15px; /* Innenabstand */ transform: rotate(-0deg); /* Dreht das Ribbon */ transform-origin: 0 0; /* Setzt den Drehpunkt */ font-size: 14px; /* Schriftgröße */ box-shadow: 2px 2px 5px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .event-badge { position: relative; margin-right: 10px; border-radius: 6px; top: 0px; /* Abstand von oben */ left: 0px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #007dc5; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 2px 8px; /* Innenabstand */ font-size: 10px; /* Schriftgröße */ box-shadow: 1px 1px 2px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .btn-default { background: #007dc5; color: #fff; border-radius: 6px; line-height: 40px; margin-top: 8px; margin-left: 20px; padding: 0px 10px; } .wpcf7 form.sent .wpcf7-response-output { color: #ffffff; background-color: #46b450; } .jumpto { padding-top: 100px; /* Adjust this value to match your header's height */ margin-top: -100px; /* This should be the same value as padding-top but negative */ } .post-item { margin-bottom: 40px; position: relative; float: left; width: 100%; border: 1px solid #ccc; padding: 20px; background-color: #f8f8f8; } .post-links { background-color: #007dc5; border: 1px solid #ccc; padding: 5px 20px; } #field_1_18, #field_1_113, #field_1_20, #field_1_21, #field_1_22, #field_1_23, #field_1_24, #field_1_25, #field_1_26, #field_1_27, #field_1_49, #field_1_51, #field_1_52, #field_1_53, #field_1_54, #field_1_56, #field_1_57, #field_1_58, #field_1_59, #field_1_60, #field_1_62, #field_1_63, #field_1_64, #field_1_65, #field_1_67, #field_1_68, #field_1_70, #field_1_72, #field_1_98, #field_1_99, #field_1_100, #field_1_101, #field_1_102, #field_1_103, #field_1_104, #field_1_107, #field_1_108, #field_1_122, #field_1_123, #field_1_124, #field_1_125, #field_1_126, #field_1_127, #field_1_128, #field_1_136 { background-color: #E8E8E8; padding: 20px 30px; border: 1px solid #C4C4C4; border-bottom: 1px solid #C4C4C4; margin-top: -20px; } #field_1_73, #field_1_74, #field_1_75, #field_1_76, #field_1_77, #field_1_78, #field_1_82, #field_1_83, 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class="section mcb-section mfn-default-section mcb-section-36258408d" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-36258408d"><div class="wrap mcb-wrap mcb-wrap-3d068616d one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-3d068616d mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-42d74db8b one laptop-one tablet-one mobile-one column_column column-margin-20px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-42d74db8b mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""> </div></div></div></div></div></div></section><section class="section mcb-section mfn-default-section mcb-section-38fac219b default-width" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-38fac219b"><div class="wrap mcb-wrap mcb-wrap-2a4f9aa06 one-fourth tablet-one-fourth laptop-one-fourth mobile-one sticky-desktop clearfix" data-desktop-col="one-fourth" data-laptop-col="laptop-one-fourth" data-tablet-col="tablet-one-fourth" data-mobile-col="mobile-one" style="--sticky-offset-desktop:110px" ><div class="mcb-wrap-inner mcb-wrap-inner-2a4f9aa06 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-3dfcdd07c one tablet-one laptop-one mobile-one mfn-item-custom-width mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-3dfcdd07c mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-fc6077eef one laptop-one tablet-one mobile-one column_table_of_contents" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-fc6077eef mcb-item-table_of_contents-inner"><div class="table_of_content clearfix " data-tags="H2"><div class="title"><h4 class="title-inner">Table of contents</h4></div><div class="table_of_content_wrapper"><ol class="mfn_toc_numbers"><li data-depth="1" ><i></i><a class="scroll" href="#mrna-dna">mRNA/DNA</a></li><li><i></i><a class="scroll" href="#viruses-inactivated-or-live-attenuated">Viruses (inactivated or live-attenuated)</a></li><li><i></i><a class="scroll" href="#virus-like-particles-vlps">Virus-like particles (VLPs)</a></li><li><i></i><a class="scroll" href="#polysaccharide-and-glycoconjugate-vaccines">Polysaccharide and glycoconjugate vaccines</a></li></ol></div></div> </div></div><div class="column mcb-column mcb-item-e026596bf one laptop-one tablet-one mobile-one column_button" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-e026596bf mcb-item-button-inner"><a class="button button_full_width button_theme button_size_2" href="/products/request-product-info.html" target="_blank" title=""><span class="button_label">Request Product Information</span></a> </div></div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-a61040b2a three-fourth tablet-three-fourth laptop-three-fourth mobile-one clearfix" data-desktop-col="three-fourth" data-laptop-col="laptop-three-fourth" data-tablet-col="tablet-three-fourth" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-a61040b2a mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div id="introduction" class="wrap mcb-wrap mcb-wrap-6a28512a1 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-6a28512a1 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-28d413b3c one laptop-one tablet-one mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-28d413b3c mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h1>Solutions for vaccine characterization and development</h1></div></div></div><div class="column mcb-column mcb-item-08pqa1c5d three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-08pqa1c5d mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>To ensure safe and efficacious vaccines, it is essential to implement robust and reliable characterization tools across discovery, development, production and quality control.</p> <p><b>Wyatt’s suite of solutions analyzes critical vaccine attributes, including:</b></p> <ul> <li>Molar mass and size</li> <li>Viral physical titer and nucleic acid content</li> <li>Aggregation</li> <li>Thermal and colloidal stability</li> </ul> <p>Many of these assays can be streamlined and automated for high throughput.</p> <p>Our technologies are also helpful in vaccine research and process development, for studying the binding affinity and stoichiometry of antibody-antigen complexes, the oligomeric state of surface glycoproteins, or optimizing formulation conditions.</p> <p>Continue reading to learn how Wyatt products are applied to characterization of specific vaccine classes.</p> </div></div></div><div class="column mcb-column mcb-item-tmui8nr9d two-fifth laptop-two-fifth tablet-two-fifth mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-tmui8nr9d mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid aligncenter no_border" style="margin-bottom:10px;"><div class="image_wrapper "><img class="scale-with-grid" src="/wp-content/uploads/2020/03/Researcher-Pipetting-825.jpg" alt="Vaccine Characterization Solutions" title="" width="" height="" style=""/></div></div> </div></div><div class="column mcb-column mcb-item-qaabkuto one laptop-one tablet-one mobile-one column_html" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-qaabkuto mcb-item-html-inner"><div class="mfn-html-editor-wrapper"><style> .item-container { display: flex; flex-wrap: wrap; gap: 20px; justify-content: space-between; width: 100%; } .responsive-item { flex: 1 1 calc(50% - 20px); /* Two columns */ display: flex; flex-direction: column; border: 4px solid #EBEDEF; padding: 20px; box-sizing: border-box; height: auto; } .responsive-item-content { display: flex; flex-direction: row; /* Image left, text right */ gap: 20px; /* Adds space between image and text */ align-items: flex-start; /* Ensures the image is top-aligned */ flex-grow: 1; } .responsive-item img { width: 100px; /* Fixed width */ height: auto; /* Maintain aspect ratio */ flex-shrink: 0; /* Prevents the image from resizing */ object-fit: contain; /* Ensures the image is not stretched */ } .text-container { display: flex; flex-direction: column; justify-content: space-between; /* Ensures the button stays at the bottom */ flex-grow: 1; height: 100%; /* Ensures all containers have the same height */ } .text-container p { font-size: 16px; line-height: 1.5; margin-bottom: 10px; flex-grow: 1; /* Pushes the button to the bottom */ } .cta-box-btn { display: inline-block; padding: 6px 20px; color: white; text-decoration: none; border-radius: 5px; font-size: 16px; width: fit-content; /* Prevents button from stretching */ align-self: flex-start; /* Ensures the button is left-aligned */ } @media (max-width: 768px) { .responsive-item { flex: 1 1 100%; /* Stacks items on smaller screens */ } .responsive-item-content { flex-direction: column; /* Stacks image and text */ } .responsive-item img { margin-bottom: 10px; width: 100%; /* Makes the image full-width on mobile */ } } </style> <div class="item-container">  <div class="responsive-item"> <div class="responsive-item-content"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-white-paper-wp9007.webp" alt="Thumbnail"> <div class="text-container"> <h4>White Paper</h4> <p> Our white paper <i>WP9007: Characterizing Vaccines with Light Scattering</i> is also available for download below. </p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/white-papers/WP9007-Characterizing-Vaccines-with-Light-Scattering.pdf" class="cta-box-btn" target="_blank" rel="noopener noreferrer"> Download White Paper </a> </div> </div> </div>  <div class="responsive-item"> <div class="responsive-item-content"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-publication-list-pl9006.webp" alt="Thumbnail"> <div class="text-container"> <h4>Publication List</h4> <p> <i>PL9006: Key publications on the characterization of vaccines<br />with multi-angle and dynamic light scattering</i> </p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/others/PL9006-Key-publications-on-the-characterization-of-vaccines-by-light-scattering.pdf" class="cta-box-btn" target="_blank" rel="noopener noreferrer"> Download Publication List </a> </div> </div> </div> </div></div></div></div></div></div><div id="aav-analytical-methods-based-on-light-scattering" class="wrap mcb-wrap mcb-wrap-4ca0281b8 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-4ca0281b8 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-c73f769c0 one laptop-one tablet-one mobile-one column_column column-margin-10px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-c73f769c0 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 style="border-bottom: thick solid #ffb600;padding-bottom:15px;">mRNA/DNA</h2> </div></div></div><div class="column mcb-column mcb-item-8a111f24d one-second laptop-one-second tablet-one-second mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-8a111f24d mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>Vaccines based on mRNA or DNA are often delivered via lipid nanoparticles, virus-like particles or other nanoparticle vectors. Multi-angle and dynamic light scattering (MALS, DLS) are useful in characterizing the mRNA itself, the carrier particle, and the complete vaccine.</p> <h3>mRNA biophysical attributes</h3> <p>As described in application note <i>AN1616: SEC-MALS methods for characterizing mRNA</i>, key biophysical attributes of mRNA are quantified by <a href="/solutions/properties/molar-mass-by-multi-angle-light-scattering-sec-mals.html">SEC-MALS</a> plus <a href="/solutions/properties/nanoparticle-and-macromolecular-size-by-light-scattering.html">DLS</a>. This analysis determines molecular weight, percentage of aggregates, radius of gyration <i>R</i><sub>g</sub>, and hydrodynamic radius <i>R</i><sub>h</sub>. The relationship between MW and <i>R</i><sub>g</sub> identifies single- or double-stranded RNA, while the ratio <i>R</i><sub>g</sub>:<i>R</i><sub>h</sub> indicates molecular conformation.</p> <p>This method can be readily adopted to further study how these attributes are affected by mRNA preparation, formulation, and storage conditions.</p></div></div></div><div class="column mcb-column mcb-item-8964dea48 one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-8964dea48 mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid aligncenter no_border" style="margin-bottom:-10px;"><div class="image_wrapper "><img width="750" height="475" src="https://www.wyatt.com/wp-content/uploads/2020/03/Biophysical-Attributes-Graph-Update-2.jpg" class="scale-with-grid" alt="SEC-MALS-DLS determines correct molecular weight and size values for mRNA, even though the molecules elute quite differently than globular protein standards such as thyroglobulin." style="" decoding="async" fetchpriority="high" srcset="https://www.wyatt.com/wp-content/uploads/2020/03/Biophysical-Attributes-Graph-Update-2.jpg 750w, https://www.wyatt.com/wp-content/uploads/2020/03/Biophysical-Attributes-Graph-Update-2-300x190.jpg 300w, https://www.wyatt.com/wp-content/uploads/2020/03/Biophysical-Attributes-Graph-Update-2-231x146.jpg 231w, https://www.wyatt.com/wp-content/uploads/2020/03/Biophysical-Attributes-Graph-Update-2-50x32.jpg 50w, https://www.wyatt.com/wp-content/uploads/2020/03/Biophysical-Attributes-Graph-Update-2-118x75.jpg 118w" sizes="(max-width:767px) 480px, 750px" /></div><p class="wp-caption-text">SEC-MALS-DLS determines correct molecular weight and size values for mRNA, even though the molecules elute quite differently than globular protein standards such as thyroglobulin.</p></div> </div></div><div class="column mcb-column mcb-item-uyivahu6p one laptop-one tablet-one mobile-one column_html" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-uyivahu6p mcb-item-html-inner"><div class="mfn-html-editor-wrapper"> <div class="responsive-container"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-app-note-1616.webp" alt="Thumbnail"> <div> <h4>Application Note AN1616</h4> <p style="margin: 0 0 10px; font-size: 16px; line-height: 1.5;"> <i></i>SEC-MALS Method for Characterizing mRNA</i> Biophysical Attributes</p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-biopolymers/AN1616-SEC-MALS-method-for-characterizing-mRNA.pdf" style="" class="cta-box-btn" target="_blank" rel="noopener noreferrer"> Download AN1616 </a> </div> </div></div></div></div><div class="column mcb-column mcb-item-zdyiapnfk one-second laptop-one-second tablet-one-second mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-zdyiapnfk mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Characterization of LNP delivery vehicles</h3> <p>Lipid nanoparticles (LNPs) include nano-emulsions, micelles, and solid lipid nanoparticles. One of the most important parameters for uptake in the lymph node, to target the antigen-presenting cells, is size. Only LNPs smaller than about 150 nm in diameter are taken up, while larger LNPs are retained at the injection site. This presents a narrow window of size for the best-performing LNPs.</p> <p><a href="/solutions/techniques/fff-mals-characterization-of-nanoparticles-colloids-macromolecules.html">FFF-MALS</a> determines high-resolution size distributions, quantifying particle concentration at each size fraction, in order to qualify precisely the efficacy of a LNP mRNA carrier. Wyatt Technology provides turn-key FFF-MALS systems based on the <a href="/products/instruments/eclipse-af4-field-flow-fractionation-system.html">Eclipse<sup>™</sup> FFF controller</a> and <a href="/products/instruments/dawn-multi-angle-light-scattering-detector.html">DAWN<sup>™</sup> multi-angle light scattering detector</a>, with comprehensive software for method development, instrument control, data acquisition and analysis.</p></div></div></div><div class="column mcb-column mcb-item-3ff9f7001 one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-3ff9f7001 mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid aligncenter no_border" style="margin-bottom:-10px;"><div class="image_wrapper "><img class="scale-with-grid" src="/wp-content/uploads/2020/03/SEC-MALS-Vaccine-Page.jpg" alt="A complete SEC-MALS system comprises the HPLC including the UV detector, SEC column(s), MALS and RI detectors. Control, data acquisition and analysis are performed by ASTRA™ software." title="" width="" height="" style=""/></div><p class="wp-caption-text">A complete SEC-MALS system comprises the HPLC including the UV detector, SEC column(s), MALS and RI detectors. Control, data acquisition and analysis are performed by ASTRA™ software.</p></div> </div></div><div class="column mcb-column mcb-item-na6unz3nk one laptop-one tablet-one mobile-one column_html" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-na6unz3nk mcb-item-html-inner"><div class="mfn-html-editor-wrapper"> <div class="responsive-container"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-white-paper-wp2608.webp" alt="Thumbnail"> <div> <h4>White Paper</h4> <p style="margin: 0 0 10px; font-size: 16px; line-height: 1.5;"> For more details on the applications of FFF-MALS in LNP characterization, see <i>WP2608: Lipid nanoparticle and liposome characterization with FFF-MALS</i>.</p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/white-papers/WP2608-Lipid-Nanoparticle-and-Liposome-Characterization-with-FFF-MALS-DLS.pdf" style="" class="cta-box-btn" target="_blank" rel="noopener noreferrer">Download WP2608 </a> </div> </div></div></div></div><div class="column mcb-column mcb-item-oglhqtk989 one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-oglhqtk989 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>LNP process development and analytics</h3> <p>Once a successful LNP/mRNA platform has been developed, scaling up the process is facilitated by <a href="/solutions/techniques/real-time-mals.html">real-time multi-angle light scattering (RT-MALS)</a>. With <a href="/products/instruments/ultradawn.html">ultraDAWN<sup>™</sup></a> either in-line or on-line, continuous monitoring of critical quality attributes like size and molar mass is achieved.</p> </div></div></div><div class="column mcb-column mcb-item-xqs8svbqa one laptop-one tablet-one mobile-one column_html" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-xqs8svbqa mcb-item-html-inner"><div class="mfn-html-editor-wrapper"> <div class="responsive-container"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-app-note-8006.webp" alt="Thumbnail"> <div> <h4>Application Note AN8006</h4> <p style="margin: 0 0 10px; font-size: 16px; line-height: 1.5;"> Real-time monitoring of liposome size in a production environment is described in <i>AN8006: In line monitoring of liposome size by RT-MALS</i>.</p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/rt-mals/AN8006-In-line-monitoring-of-liposome-size-by-RT-MALS.pdf" style="" class="cta-box-btn" target="_blank" rel="noopener noreferrer"> Download AN8006 </a> </div> </div></div></div></div></div></div><div id="aav-sop-guidance-manual" class="wrap mcb-wrap mcb-wrap-b0f162c4a one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-b0f162c4a mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-d537c777f one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-d537c777f mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 style="border-bottom: thick solid #ffb600;padding-bottom:15px;">Viruses (inactivated or live-attenuated)</h2></div></div></div><div class="column mcb-column mcb-item-078ee45af one-second laptop-one-second tablet-one-second mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-078ee45af mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>These complex vaccine products combine multiple serotypes, often with adjuvants. Asymmetric-flow field-flow fractionation (AF4) separates such complex samples by size with high resolution. Fractionation is followed downstream by multiple online instruments, including MALS, DLS, UV and fluorescence detectors, to complete the biophysical characterization.</p> <h3>Product validation</h3> <p>In the analysis of an influenza virus subpopulation shown here, both size and total particle concentration are quantified in detail by <a href="/solutions/techniques/fff-mals-characterization-of-nanoparticles-colloids-macromolecules.html">FFF-MALS</a>. Comparison with results from TEM shows excellent agreement within 2% in virus concentration and 5% in size. A small dimer peak is also identified.</p> <p>Complementary to FFF-MALS, the <a href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html">DynaPro<sup>™</sup> Plate Reader</a> provides high-throughput screening of hundreds of samples with respect to processing conditions and formulations. In addition to sizing, this technique is able to screen for thermal stability of virus samples and to determine viral particle concentration.</p> <h3>Additional literature</h3> <p>See <a href="https://doi.org/10.1016/J.JVIROMET.2007.04.008" target="_blank" rel="noopener">http://doi.org/10.1016/J.JVIROMET.2007.04.008</a> to explore the comparison of FFF-MALS analysis to other methods such as transmission electron microscopy (TEM), atomic force microscopy (AFM), size exclusion chromatography followed by MALS (SEC-MALS), quantitative reverse transcription polymerase chain reaction (RT Q-PCR), median tissue culture dose (TCID50), and the fluorescent focus assay (FFA).</p></div></div></div><div class="column mcb-column mcb-item-640591ad6 one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-640591ad6 mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid aligncenter no_border" style="margin-bottom:-10px;"><div class="image_wrapper "><img class="scale-with-grid" src="/wp-content/uploads/2020/03/Nanoparticle-Number-Densities-Graph-750-update.jpg" alt="FFF-MALS is an excellent tool for vaccine characterization. Total viral count and size may be quantified with high resolution for each subpopulation." title="" width="" height="" style=""/></div><p class="wp-caption-text">FFF-MALS is an excellent tool for vaccine characterization. Total viral count and size may be quantified with high resolution for each subpopulation.</p></div> </div></div></div></div><div id="aav-sop-guidance-manual" class="wrap mcb-wrap mcb-wrap-z2kqwpmd one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-z2kqwpmd mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-191b46bfd one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-191b46bfd mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 style="border-bottom: thick solid #ffb600;padding-bottom:15px;">Virus-like particles (VLPs)</h2></div></div></div><div class="column mcb-column mcb-item-35e16bde3 one-second laptop-one-second tablet-one-second mobile-one column_column column-margin-10px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-35e16bde3 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>Recombinant virus-like particles, which are designed to elicit an immune response in the same way that a traditional live-attenuated vaccine does, are typically comprised of a protein capsid, with nucleic acid cargo. SEC-MALS and FFF-MALS characterize both the capsid and cargo, while DLS is suitable as a quality-control tool to determine size, aggregation and viral physical titer.</p> <h3>Monitoring production process: measuring protein capsid and DNA cargo</h3> <p>The synthesis of VLPs involves three steps: 1) isolation of recombinant VLP from cell culture, 2) dissociation of the virus into pentameric capsomeres with removal of all DNA, 3) introduction of the DNA of interest and packaging into a newly formed VLP.</p> <p>FFF-MALS is used to monitor this process, by separating and detecting fully formed capsids from aggregates, unassembled protomers, and capsomeres. The analysis informs on conformation, and, in the final step of the process, simultaneously measures protein and DNA molar mass to quantify the payload.</p> <h3>Additional literature</h3> <p><i>PL2609: FFF-MALS-DLS nanoDDS key publications</i> contains references to publications highlighting the use of FFF-MALS to characterize liposomes, polymersomes, virus-like particles and other potential gene vectors delivery vehicles.</p></div></div></div><div class="column mcb-column mcb-item-7a0f90b43 one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-7a0f90b43 mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid aligncenter no_border" style="margin-bottom:-10px;"><div class="image_wrapper "><img class="scale-with-grid" src="/wp-content/uploads/2020/03/VLP-Graph-Vaccine-Solutions-750-update.jpg" alt="ASTRA software simultaneously determines molar mass distribution for VLP and protein to quantify DNA payload." title="" width="" height="" style=""/></div><p class="wp-caption-text">ASTRA software simultaneously determines molar mass distribution for VLP and protein to quantify DNA payload.</p></div> </div></div><div class="column mcb-column mcb-item-7bebb4671 one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-7bebb4671 mcb-item-image-inner"><div class="image_frame image_item scale-with-grid aligncenter no_border hover-disable" style="margin-bottom:-10px;" role="link" aria-label="Image with links" tabindex="0"><div class="image_wrapper"><a href="https://www.wyatt.com/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html" target="_blank" class="" tabindex="-1" title=""><div class="mask"></div><img class="scale-with-grid" src="/wp-content/uploads/2020/03/Plate-Reader-Vaccine-Page.jpg" alt="The DynaPro Plate Reader enables rapid screening of formulations for size and stability." title="" width="" height="" style=""/></a><div class="image_links"><a href="https://www.wyatt.com/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html" class="link " target="_blank" tabindex="-1" title=""><svg viewBox="0 0 26 26" aria-label="go to link"><defs><style>.path{fill:none;stroke:#333;stroke-miterlimit:10;stroke-width:1.5px;}</style></defs><g><path d="M10.17,8.76l2.12-2.12a5,5,0,0,1,7.07,0h0a5,5,0,0,1,0,7.07l-2.12,2.12" class="path"></path><path d="M15.83,17.24l-2.12,2.12a5,5,0,0,1-7.07,0h0a5,5,0,0,1,0-7.07l2.12-2.12" class="path"></path><line x1="10.17" y1="15.83" x2="15.83" y2="10.17" class="path"></line></g></svg></a></div></div><p class="wp-caption-text">The DynaPro Plate Reader enables rapid screening of formulations for size and stability.</p></div> </div></div><div class="column mcb-column mcb-item-b2zn29sf two-fifth laptop-two-fifth tablet-two-fifth mobile-one column_button" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-b2zn29sf mcb-item-button-inner"><a class="button button_full_width button_size_2" href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/others/PL2609-FFF-MALS-DLS-nanoDDS-key-publications.pdf" style="background-color:#0065a4!important;color:#ffffff;" target="_blank" title=""><span class="button_label">Download PL2609</span></a> </div></div><div class="column mcb-column mcb-item-ebbe7dde2 one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-ebbe7dde2 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>View the webinar <i>VLP Characterization: The Light Scattering Biophysical Toolbox</i> for additional means of characterizing VLPs and their interactions with neutralizing antibodies.</p></div></div></div><div class="column mcb-column mcb-item-loltho7ed two-fifth laptop-two-fifth tablet-two-fifth mobile-one column_button" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-loltho7ed mcb-item-button-inner"><a class="button button_full_width button_size_2" href="/library/webinars/vlp-characterization-light-scattering-biophysical-toolbox.html" style="background-color:#0065a4!important;color:#ffffff;" target="_blank" title=""><span class="button_label">View Webinar</span></a> </div></div><div class="column mcb-column mcb-item-a3e239c70 one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-a3e239c70 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><strong>High-throughput screening</strong> <p>See <a href="https://doi.org/10.1016/j.bej.2015.04.004" target="_blank">http://doi.org/10.1016/j.bej.2015.04.004</a> to learn about a rapid and simple screening method, based on high-throughput dynamic light scattering using the <a href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html">DynaPro Plate Reader</a>, used to identify conditions for enhanced stability of modular vaccine candidates consisting of capsomeres.</p></div></div></div><div class="column mcb-column mcb-item-dso9v7m9j two-fifth laptop-two-fifth tablet-two-fifth mobile-one column_button" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-dso9v7m9j mcb-item-button-inner"><a class="button button_full_width button_size_2" href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html" style="background-color:#0065a4!important;color:#ffffff;" target="_blank" title=""><span class="button_label">Instrument Information</span></a> </div></div></div></div><div id="aav-sop-guidance-manual" class="wrap mcb-wrap mcb-wrap-8sxsvl24q one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-8sxsvl24q mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-4h4r5kf1m one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-4h4r5kf1m mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 style="border-bottom: thick solid #ffb600;padding-bottom:15px;">Polysaccharide and glycoconjugate vaccines</h2></div></div></div><div class="column mcb-column mcb-item-8e0ad3bf5 one-second laptop-one-second tablet-one-second mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-8e0ad3bf5 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>Polysaccharide and protein glycoconjugate vaccines combine adjuvants with high-molecular-weight polysaccharide antigens, typically including several serotypes. In protein glycoconjugates, protein adjuvants are covalently linked directly to the polysaccharide.</p> <p>Since molecular weight is central to the immunogenicity of this type of vaccine, SEC-MALS and FFF-MALS are essential for their characterization and quality control.</p> <h3>Size and molar mass characterization</h3> <p>FFF‐MALS, combining an <a href="/products/instruments/eclipse-af4-field-flow-fractionation-system.html">Eclipse FFF controller</a>, <a href="/products/instruments/dawn-multi-angle-light-scattering-detector.html">DAWN MALS detector</a>, <a href="/products/instruments/optilab-refractive-index-detector.html">Optilab<sup>™</sup> dRI detector</a> and industry-standard HPLC components, is the preferred means of characterizing protein-polysaccharide conjugate vaccines that extend above tens or hundreds of MDa in MW. As there is no column matrix, there is no shear‐induced degradation while fractionating the entire range; MALS determines molar mass and size simultaneously.</p> <p>For smaller molecules, SEC-MALS may be more readily utilized. Typically, SEC-MALS can determine not only the overall molar mass of the molecule, but also quantify the content of protein and content of polysaccharide in each eluting fraction. A SEC-MALS system includes HPLC pump, autosampler and column, a DAWN and Optilab, and may also include a <a href="/products/instruments/viscostar-online-differential-viscometer.html">ViscoStar<sup>™</sup> differential viscometer</a> for additional analysis of conformation.</p></div></div></div><div class="column mcb-column mcb-item-611f1882c one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-611f1882c mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid aligncenter no_border" style="margin-bottom:-10px;"><div class="image_wrapper "><img class="scale-with-grid" src="/wp-content/uploads/2020/03/Protein-Polysaccharide-Conjugates-Graph-750-update.jpg" alt="FFF-MALS precisely characterizes molar mass and size distributions for large protein-polysaccharide conjugates." title="" width="" height="" style=""/></div><p class="wp-caption-text">FFF-MALS precisely characterizes molar mass and size distributions for large protein-polysaccharide conjugates.</p></div> </div></div><div class="column mcb-column mcb-item-9e7bcfd70 one-second laptop-one-second tablet-one-second mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-9e7bcfd70 mcb-item-image-inner"><div class="image_frame image_item scale-with-grid aligncenter no_border hover-disable" role="link" aria-label="Image with links" tabindex="0"><div class="image_wrapper"><a href="https://www.wyatt.com/products/instruments/dawn-multi-angle-light-scattering-detector.html" target="_blank" class="" tabindex="-1" title=""><div class="mask"></div><img class="scale-with-grid" src="/wp-content/uploads/2020/03/DAWN-Vaccine-LP.jpg" alt="DAWN MALS detector quantifies molar masses up to hundreds of MDa." title="" width="" height="" style=""/></a><div class="image_links"><a href="https://www.wyatt.com/products/instruments/dawn-multi-angle-light-scattering-detector.html" class="link " target="_blank" tabindex="-1" title=""><svg viewBox="0 0 26 26" aria-label="go to link"><defs><style>.path{fill:none;stroke:#333;stroke-miterlimit:10;stroke-width:1.5px;}</style></defs><g><path d="M10.17,8.76l2.12-2.12a5,5,0,0,1,7.07,0h0a5,5,0,0,1,0,7.07l-2.12,2.12" class="path"></path><path d="M15.83,17.24l-2.12,2.12a5,5,0,0,1-7.07,0h0a5,5,0,0,1,0-7.07l2.12-2.12" class="path"></path><line x1="10.17" y1="15.83" x2="15.83" y2="10.17" class="path"></line></g></svg></a></div></div><p class="wp-caption-text">DAWN MALS detector quantifies molar masses up to hundreds of MDa.</p></div> </div></div><div class="column mcb-column mcb-item-fg77znst one laptop-one tablet-one mobile-one column_html" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-fg77znst mcb-item-html-inner"><div class="mfn-html-editor-wrapper"> <div class="responsive-container"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb_webinars.jpg" alt="Thumbnail"> <div> <h4>Webinar</h4> <p style="margin: 0 0 10px; font-size: 16px; line-height: 1.5;"> More details on the analysis of protein-polysaccharide conjugate vaccines and other vaccine types are explained in our webinar <i>Vaccines, Well Characterized? Light scattering solutions for biophysical characterization in vaccine R&D</i>.</p> <a href="https://www.wyatt.com/library/webinars/biophysical-characterization-vaccine-research.html" style="" class="cta-box-btn" target="_blank" rel="noopener noreferrer"><i class="fa-regular fa-circle-play" style="color: #ffffff; font-size: 15px;"></i> View Webinar </a> </div> </div></div></div></div><div class="column mcb-column mcb-item-4s0hxyij one laptop-one tablet-one mobile-one column_html" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-4s0hxyij mcb-item-html-inner"><div class="mfn-html-editor-wrapper"><style> .item-container { display: flex; flex-wrap: wrap; gap: 20px; justify-content: space-between; width: 100%; } .responsive-item { flex: 1 1 calc(50% - 20px); /* Two columns */ display: flex; flex-direction: column; border: 4px solid #EBEDEF; padding: 20px; box-sizing: border-box; height: auto; } .responsive-item-content { display: flex; flex-direction: row; /* Image 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.responsive-item { flex: 1 1 100%; /* Stacks items on smaller screens */ } .responsive-item-content { flex-direction: column; /* Stacks image and text */ } .responsive-item img { margin-bottom: 10px; width: 100%; /* Makes the image full-width on mobile */ } } </style> <div class="item-container">  <div class="responsive-item"> <div class="responsive-item-content"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-app-note-8005.webp" alt="Thumbnail"> <div class="text-container"> <h4>Application Note AN8005</h4> <p> <i>AN8005: RT-MALS end-point determination of a polysaccharide depolymerization process</i> describes how the ultraDAWN real-time MALS detector monitors polysaccharide molecular weight during processing to ensure optimal quality on the production floor. </p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/rt-mals/AN8005-RT-MALS-end-point-determination-of-a-polysaccharide-depolymerization-process.pdf" class="cta-box-btn" target="_blank" rel="noopener noreferrer"> Read the Application Note </a> </div> </div> </div>  <div class="responsive-item"> <div class="responsive-item-content"> <img src="https://www.wyatt.com/wp-content/uploads/2024/11/thumb-app-note-1306.webp" alt="Thumbnail"> <div class="text-container"> <h4>Application Note AN1306</h4> <p> <i>AN1306: Polyvalent pneumococcal polysaccharide vaccine characterized by SEC-MALS</i> describes how MALS can be used to aid quality control in vaccine QC. </p> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-biopolymers/AN1306-Polyvalent-pneumococcal-polysaccharide-vaccine-by-SEC-MALS.pdf" class="cta-box-btn" target="_blank" rel="noopener noreferrer"> Read the Application Note </a> </div> </div> </div> </div></div></div></div></div></div><div id="process-analytical-technology-for-lnps" class="wrap mcb-wrap mcb-wrap-9d583a282 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-9d583a282 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div></div></div></div></div></div></section><section class="section mcb-section mfn-default-section mcb-section-c3af19d9c" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-c3af19d9c"><div class="wrap mcb-wrap mcb-wrap-ea041bbb0 one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-ea041bbb0 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div></div></div></div></section></div><section class="section mcb-section the_content no_content"><div class="section_wrapper"><div class="the_content_wrapper "></div></div></section> <section class="section section-page-footer"> <div class="section_wrapper clearfix"> <div class="column one page-pager"> <div class="mcb-column-inner"> </div> </div> </div> </section> </div> </main> </div> </div>  <section class="section before-footer mcb-section mfn-default-section mcb-section-arexmxc3 default-width" style="padding-top:70px;"><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-arexmxc3"><div class="wrap mcb-wrap mcb-wrap-jana9ffxy container one-third tablet-one-third laptop-one-third mobile-one clearfix" data-desktop-col="one-third" data-laptop-col="laptop-one-third" data-tablet-col="tablet-one-third" data-mobile-col="mobile-one" style="padding-right:20px;"><div class="mcb-wrap-inner mcb-wrap-inner-jana9ffxy mfn-module-wrapper mfn-wrapper-for-wraps"><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-xp9n3fr84 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style=""><div class="mcb-wrap-inner mcb-wrap-inner-xp9n3fr84 mfn-module-wrapper mfn-wrapper-for-wraps"><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-wcj43y7s one laptop-one tablet-one mobile-one column_column" style=""><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-wcj43y7s mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style="padding-bottom:30px;"> <h4>Ultimate Guide</h4><div class="textwidget custom-html-widget"><p style="min-height:75px;">Thirteen key questions to ask when buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument.</p> <a class="button button_size_2" href="/library/ultimate-guide-request.html" style="background-color:#fdbb30!important;color:#000000;" title=""><span class="button_label">Request Ultimate Guide</span></a> </div> </div></div></div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-1e21iwv44 one-third tablet-one-third laptop-one-third mobile-one clearfix" data-desktop-col="one-third" data-laptop-col="laptop-one-third" data-tablet-col="tablet-one-third" data-mobile-col="mobile-one" style=""><div class="mcb-wrap-inner mcb-wrap-inner-1e21iwv44 mfn-module-wrapper mfn-wrapper-for-wraps"><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-y8xi6b5x8 container one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding-right:20px;"><div class="mcb-wrap-inner mcb-wrap-inner-y8xi6b5x8 mfn-module-wrapper mfn-wrapper-for-wraps"><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-tny554c6n one laptop-one tablet-one mobile-one 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