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analysis</title> <meta name="description" content="Search results for: HPLC analysis"> <meta name="keywords" content="HPLC analysis"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="HPLC analysis"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 28050</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: HPLC analysis</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28050</span> Optimized Simultaneous Determination of Theobromine and Caffeine in Fermented and Unfermented Cacao Beans and in Cocoa Products Using Step Gradient Solvent System in Reverse Phase HPLC</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ian%20Marc%20G.%20Cabugsa">Ian Marc G. Cabugsa</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20Ryan%20A.%20Won"> Kim Ryan A. Won</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fast, reliable and simultaneous HPLC analysis of theobromine and caffeine in cacao and cocoa products was optimized in this study. The samples tested were raw, fermented, and roasted cacao beans as well as commercially available cocoa products. The HPLC analysis was carried out using step gradient solvent system with acetonitrile and water buffered with H3PO4 as the mobile phase. The HPLC system was optimized using 273 nm wavelength at 35 °C for the column temperature with a flow rate of 1.0 mL/min. Using this method, the theobromine percent recovery mean, Limit of Detection (LOD) and Limit of Quantification (LOQ) is 118.68(±3.38)%, 0.727 and 1.05 respectively. The percent recovery mean, LOD and LOQ for caffeine is 105.53(±3.25)%, 2.42 and 3.50 respectively. The inter-day and intra-day precision for theobromine is 4.31% and 4.48% respectively, while 7.02% and 7.03% was for caffeine respectively. Compared to the standard method in AOAC using methanol in isocratic solvent system, the results of the study produced lesser chromatogram noise with emphasis on theobromine and caffeine. The method is readily usable for cacao and cocoa substances analyses using HPLC with step gradient capability. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cacao" title="cacao">cacao</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=step%20gradient%20solvent%20system" title=" step gradient solvent system"> step gradient solvent system</a>, <a href="https://publications.waset.org/abstracts/search?q=theobromine" title=" theobromine"> theobromine</a> </p> <a href="https://publications.waset.org/abstracts/43155/optimized-simultaneous-determination-of-theobromine-and-caffeine-in-fermented-and-unfermented-cacao-beans-and-in-cocoa-products-using-step-gradient-solvent-system-in-reverse-phase-hplc" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43155.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28049</span> Analytical Method Development and Validation of Stability Indicating Rp - Hplc Method for Detrmination of Atorvastatin and Methylcobalamine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alkaben%20Patel">Alkaben Patel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The proposed RP-HPLC method is easy, rapid, economical, precise and accurate stability indicating RP-HPLC method for simultaneous estimation of Astorvastatin and Methylcobalamine in their combined dosage form has been developed.The separation was achieved by LC-20 AT C18(250mm*4.6mm*2.6mm)Colum and water (pH 3.5): methanol 70:30 as mobile phase, at a flow rate of 1ml/min. wavelength of this dosage form is 215nm.The drug is related to stress condition of hydrolysis, oxidation, photolysis and thermal degradation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=RP-%20HPLC" title="RP- HPLC">RP- HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=atorvastatin" title=" atorvastatin"> atorvastatin</a>, <a href="https://publications.waset.org/abstracts/search?q=methylcobalamine" title=" methylcobalamine"> methylcobalamine</a>, <a href="https://publications.waset.org/abstracts/search?q=method" title=" method"> method</a>, <a href="https://publications.waset.org/abstracts/search?q=development" title=" development"> development</a>, <a href="https://publications.waset.org/abstracts/search?q=validation" title=" validation"> validation</a> </p> <a href="https://publications.waset.org/abstracts/45955/analytical-method-development-and-validation-of-stability-indicating-rp-hplc-method-for-detrmination-of-atorvastatin-and-methylcobalamine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45955.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">335</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28048</span> High-Performance Liquid Chromatographic Method with Diode Array Detection (HPLC-DAD) Analysis of Naproxen and Omeprazole Active Isomers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marwa%20Ragab">Marwa Ragab</a>, <a href="https://publications.waset.org/abstracts/search?q=Eman%20El-Kimary"> Eman El-Kimary</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chiral separation and analysis of omeprazole and naproxen enantiomers in tablets were achieved using high-performance liquid chromatographic method with diode array detection (HPLC-DAD). Kromasil Cellucoat chiral column was used as a stationary phase for separation and the eluting solvent consisted of hexane, isopropanol and trifluoroacetic acid in a ratio of: 90, 9.9 and 0.1, respectively. The chromatographic system was suitable for the enantiomeric separation and analysis of active isomers of the drugs. Resolution values of 2.17 and 3.84 were obtained after optimization of the chromatographic conditions for omeprazole and naproxen isomers, respectively. The determination of S-isomers of each drug in their dosage form was fully validated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chiral%20analysis" title="chiral analysis">chiral analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=esomeprazole" title=" esomeprazole"> esomeprazole</a>, <a href="https://publications.waset.org/abstracts/search?q=S-Naproxen" title=" S-Naproxen"> S-Naproxen</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC-DAD" title=" HPLC-DAD"> HPLC-DAD</a> </p> <a href="https://publications.waset.org/abstracts/61903/high-performance-liquid-chromatographic-method-with-diode-array-detection-hplc-dad-analysis-of-naproxen-and-omeprazole-active-isomers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61903.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">301</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28047</span> Development and Validation of a HPLC Method for Standardization of Methanolic Extract of Hypericum sinaicum Hochst</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Taghreed%20A.%20Ibrahim">Taghreed A. Ibrahim</a>, <a href="https://publications.waset.org/abstracts/search?q=Atef%20A.%20El-Hela"> Atef A. El-Hela</a>, <a href="https://publications.waset.org/abstracts/search?q=Hala%20M.%20El-Hefnawy"> Hala M. El-Hefnawy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The chromatographic profile of methanol extract of Hypericum sinaicum was determined using HPLC-DAD. Apigenin was used as an external standard in the development and validation of the HPLC method. The proposed method is simple, rapid and reliable and can be successfully applied for standardization of Hypericum sinaicum methanol extract. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=quality%20control" title="quality control">quality control</a>, <a href="https://publications.waset.org/abstracts/search?q=standardization" title=" standardization"> standardization</a>, <a href="https://publications.waset.org/abstracts/search?q=falvonoids" title=" falvonoids"> falvonoids</a>, <a href="https://publications.waset.org/abstracts/search?q=methanol%20extract" title=" methanol extract"> methanol extract</a> </p> <a href="https://publications.waset.org/abstracts/15989/development-and-validation-of-a-hplc-method-for-standardization-of-methanolic-extract-of-hypericum-sinaicum-hochst" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15989.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">503</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28046</span> Development and Validation of HPLC Method on Determination of Acesulfame-K in Jelly Drink Product</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Candra%20Irawan">Candra Irawan</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20Yudianto"> David Yudianto</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahsanu%20Nadiyya"> Ahsanu Nadiyya</a>, <a href="https://publications.waset.org/abstracts/search?q=Dewi%20Anna%20Br%20Sitepu"> Dewi Anna Br Sitepu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hanafi"> Hanafi</a>, <a href="https://publications.waset.org/abstracts/search?q=Erna%20Styani"> Erna Styani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Jelly drink was produced from a combination of both natural and synthetic materials, such as acesulfame potassium (acesulfame-K) as synthetic sweetener material. Acesulfame-K content in jelly drink could be determined by High-Performance Liquid Chromatography (HPLC), but this method needed validation due to having a change on the reagent addition step which skips the carrez addition and comparison of mix mobile phase (potassium dihydrogen phosphate and acetonitrile) with ratio from 75:25 to 90:10 to be more efficient and cheap. This study was conducted to evaluate the performance of determination method for acesulfame-K content in the jelly drink by HPLC. The method referred to Deutsches Institut fur Normung European Standard International Organization for Standardization (DIN EN ISO):12856 (1999) about Foodstuffs, Determination of acesulfame-K, aspartame and saccharin. The result of the correlation coefficient value (r) on the linearity test was 0.9987 at concentration range 5-100 mg/L. Detection limit value was 0.9153 ppm, while the quantitation limit value was 1.1932 ppm. The recovery (%) value on accuracy test for sample concentration by spiking 100 mg/L was 102-105%. Relative Standard Deviation (RSD) value for precision and homogenization tests were 2.815% and 4.978%, respectively. Meanwhile, the comparative and stability tests were tstat (0.136) < ttable (2.101) and |µ1-µ2| (1.502) ≤ 0.3×CV Horwitz. Obstinacy test value was tstat < ttable. It can be concluded that the HPLC method for the determination of acesulfame-K in jelly drink product by HPLC has been valid and can be used for analysis with good performance. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acesulfame-K" title="acesulfame-K">acesulfame-K</a>, <a href="https://publications.waset.org/abstracts/search?q=jelly%20drink" title=" jelly drink"> jelly drink</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=validation" title=" validation"> validation</a> </p> <a href="https://publications.waset.org/abstracts/111374/development-and-validation-of-hplc-method-on-determination-of-acesulfame-k-in-jelly-drink-product" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/111374.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">129</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28045</span> Separation and Purification of Oligostilbenes Using HPLC with Dereplication Strategy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nurhuda%20Manshoor">Nurhuda Manshoor</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohd%20Fazirulrahman%20Fathil"> Mohd Fazirulrahman Fathil</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Hakim%20Jaafar"> Muhammad Hakim Jaafar</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohd%20Amirul%20S.%20A.%20Jalil"> Mohd Amirul S. A. Jalil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The leaves of Neobalanocarpus heimii were investigated for their oligostilbene contents. Prior to isolation process, the determinations of compounds were based on mass spectrometric fragmentation patterns. Three compounds, heimiol B, hopeaphenol, and vaticaphenol A were identified directly from the crude extract. Preparative high-performance liquid chromatography (HPLC) was used to isolate and purify the other compounds. The purified compounds were then analyzed using NMR spectroscopy to identify the compound structure and stereochemistry. The method employed for the research modified to comply with different HPLC techniques such as preparative and analytical techniques. The crude sample was injected into preparative HPLC to obtain several fractions which consist of oligostilbene mixture. The fractions were further isolated using analytical HPLC to obtain four pure compounds. The compounds then were characterized using nuclear magnetic resonance (NMR). The result shows that the leaves extract of Neobalanocarpus heimii contain three oligostilbenes, namely vaticanol A, balanocarpol, and vaticaphenol A, and a galactopyranose. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=balanocarpol" title="balanocarpol">balanocarpol</a>, <a href="https://publications.waset.org/abstracts/search?q=hemiol%20B" title=" hemiol B"> hemiol B</a>, <a href="https://publications.waset.org/abstracts/search?q=hopeaphenol" title=" hopeaphenol"> hopeaphenol</a>, <a href="https://publications.waset.org/abstracts/search?q=vaticanol%20A" title=" vaticanol A"> vaticanol A</a>, <a href="https://publications.waset.org/abstracts/search?q=vaticaphenol%20A" title=" vaticaphenol A"> vaticaphenol A</a> </p> <a href="https://publications.waset.org/abstracts/18775/separation-and-purification-of-oligostilbenes-using-hplc-with-dereplication-strategy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18775.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">496</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28044</span> Method Development and Validation for Quantification of Active Content and Impurities of Clodinafop Propargyl and Its Enantiomeric Separation by High-Performance Liquid Chromatography</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kamlesh%20Vishwakarma">Kamlesh Vishwakarma</a>, <a href="https://publications.waset.org/abstracts/search?q=Bipul%20Behari%20Saha"> Bipul Behari Saha</a>, <a href="https://publications.waset.org/abstracts/search?q=Sunilkumar%20Sing"> Sunilkumar Sing</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhishek%20Mishra"> Abhishek Mishra</a>, <a href="https://publications.waset.org/abstracts/search?q=Sreenivas%20Rao"> Sreenivas Rao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A rapid, sensitive and inexpensive method has been developed for complete analysis of Clodinafop Propargyl. Clodinafop Propargyl enantiomers were separated on chiral column, Chiral Pak AS-H (250 mm. 4.6mm x 5µm) with mobile phase n-hexane: IPA (96:4) at flow rate 1.5 ml/min. The effluent was monitored by UV detector at 230 nm. Clodinafop Propagyl content and impurity quantification was done with reverse phase HPLC. The present study describes a HPLC method using simple mobile phase for the quantification of Clodinafop Propargyl and its impurities. The method was validated and found to be accurate, precise, convenient and effective. Moreover, the lower solvent consumption along with short analytical run time led to a cost effective analytical method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Clodinafop%20Propargyl" title="Clodinafop Propargyl">Clodinafop Propargyl</a>, <a href="https://publications.waset.org/abstracts/search?q=method" title=" method"> method</a>, <a href="https://publications.waset.org/abstracts/search?q=validation" title=" validation"> validation</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC-UV" title=" HPLC-UV"> HPLC-UV</a> </p> <a href="https://publications.waset.org/abstracts/63561/method-development-and-validation-for-quantification-of-active-content-and-impurities-of-clodinafop-propargyl-and-its-enantiomeric-separation-by-high-performance-liquid-chromatography" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63561.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">371</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28043</span> Olive Oils from Algeria: Phenolic Compounds Composition and Antibacterial Activity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Firdaousse%20Laincer">Firdaousse Laincer</a>, <a href="https://publications.waset.org/abstracts/search?q=Rahima%20Laribi"> Rahima Laribi</a>, <a href="https://publications.waset.org/abstracts/search?q=Abderazak%20Tamendjari"> Abderazak Tamendjari</a>, <a href="https://publications.waset.org/abstracts/search?q=Rovellini%20Venturini"> Rovellini Venturini </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Phenolic compounds present in olive oil have received much attention in recent years due to their beneficial functional and nutritional effects. Phenolic composition, antibacterial activity of phenolic extracts of olive oil varieties from Algeria were investigated. The analysis of polyphenols was performed by Folin-Ciocalteu and HPLC. As a result, many phenolic compounds were identified and quantified by using HPLC; derivatives of oleuropein and ligstroside, hydroxytyrosol, tyrosol, flavonoids, and lignans reporting unique and characteristic phenolic profile. These phenolic fractions also differentiate the total antibacterial activity. Among the bacteria tested, S. aureus and, to a lesser extent, B. subtilis showed the highest sensitivity; the MIC varied from 0.6 to 1.6 mg•mL-1 and 1.2 to 1.8 mg•mL-1, respectively. The results obtained denote that Algerian olive oils may constitute a good source of healthy compounds, phenolics compounds, in the diet, suggesting that their consumption could be useful in the prevention of diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibacterial%20activity" title="antibacterial activity">antibacterial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=olive%20oil" title=" olive oil"> olive oil</a>, <a href="https://publications.waset.org/abstracts/search?q=phenols" title=" phenols"> phenols</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a> </p> <a href="https://publications.waset.org/abstracts/13202/olive-oils-from-algeria-phenolic-compounds-composition-and-antibacterial-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13202.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">452</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28042</span> HPLC-UV Screening of Legal (Caffeine and Yohimbine) and Illegal (Ephedrine and Sibutramine) Substances from Weight Loss Dietary Supplements for Athletes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amelia%20Tero-Vescan">Amelia Tero-Vescan</a>, <a href="https://publications.waset.org/abstracts/search?q=Camil-Eugen%20Vari"> Camil-Eugen Vari</a>, <a href="https://publications.waset.org/abstracts/search?q=Laura%20Ciulea"> Laura Ciulea</a>, <a href="https://publications.waset.org/abstracts/search?q=Cristina%20Filip"> Cristina Filip</a>, <a href="https://publications.waset.org/abstracts/search?q=Silvia%20Imre"> Silvia Imre</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A HPLC –UV method for the identification of ephedrine (EPH), sibutramine (SB), yohimbine (Y) and caffeine (CF) was developed. Separation was performed on a Kromasil 100-RP8, 150 mm x 4.6 mm, 5 mm column equipped with a precolumn Kromasil RP 8. Mobile phase was a gradient of 80-35 % sodium dihydrogen phosphate pH=5 with NH4OH and acetonitrile over 15 minutes time of analysis. Based on the responses of 113 athletes about dietary supplements (DS) consumed for "fat burning" and weight loss which have a legal status in Romania, 28 supplements have been selected and investigated for their content in CF, Y, legal substances, and SB, EPH (prohibited substances in DS). The method allows quantitative determination of the four substances in a short analysis time and with minimum cost. The presence of SB and EPH in the analyzed DS was not detected while the content in CF and Y considering the dosage recommended by the manufacturer does not affect the health of the consumers. DS labeling (plant extracts with CF and Y content) allows manufacturers to avoid declaring correct and exact amounts per pharmaceutical form (pure CF or equivalent and Y, respectively). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dietary%20supplements" title="dietary supplements">dietary supplements</a>, <a href="https://publications.waset.org/abstracts/search?q=sibutramine" title=" sibutramine"> sibutramine</a>, <a href="https://publications.waset.org/abstracts/search?q=ephedrine" title=" ephedrine"> ephedrine</a>, <a href="https://publications.waset.org/abstracts/search?q=yohimbine" title=" yohimbine"> yohimbine</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeine" title=" caffeine"> caffeine</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a> </p> <a href="https://publications.waset.org/abstracts/2518/hplc-uv-screening-of-legal-caffeine-and-yohimbine-and-illegal-ephedrine-and-sibutramine-substances-from-weight-loss-dietary-supplements-for-athletes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2518.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">442</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28041</span> The Comparison and Optimization of the Analytic Method for Canthaxanthin, Food Colorants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hee-Jae%20Suh">Hee-Jae Suh</a>, <a href="https://publications.waset.org/abstracts/search?q=Kyung-Su%20Kim"> Kyung-Su Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Min-Ji%20Kim"> Min-Ji Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Yeon-Seong%20Jeong"> Yeon-Seong Jeong</a>, <a href="https://publications.waset.org/abstracts/search?q=Ok-Hwan%20Lee"> Ok-Hwan Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Jae-Wook%20Shin"> Jae-Wook Shin</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyang-Sook%20Chun"> Hyang-Sook Chun</a>, <a href="https://publications.waset.org/abstracts/search?q=Chan%20Lee"> Chan Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Canthaxanthin is keto-carotenoid produced from beta-carotene and it has been approved to be used in many countries as a food coloring agent. Canthaxanthin has been analyzed using High Performance Liquid Chromatography (HPLC) system with various ways of pretreatment methods. Four official methods for verification of canthaxanthin at FSA (UK), AOAC (US), EFSA (EU) and MHLW (Japan) were compared to improve its analytical and the pretreatment method. The Linearity, the limit of detection (LOD), the limit of quantification (LOQ), the accuracy, the precision and the recovery ratio were determined from each method with modification in pretreatment method. All HPLC methods exhibited correlation coefficients of calibration curves for canthaxanthin as 0.9999. The analysis methods from FSA, AOAC, and MLHW showed the LOD of 0.395 ppm, 0.105 ppm, and 0.084 ppm, and the LOQ of 1.196 ppm, 0.318 ppm, 0.254 ppm, respectively. Among tested methods, HPLC method of MHLW with modification in pretreatments was finally selected for the analysis of canthaxanthin in lab, because it exhibited the resolution factor of 4.0 and the selectivity of 1.30. This analysis method showed a correlation coefficients value of 0.9999 and the lowest LOD and LOQ. Furthermore, the precision ratio was lower than 1 and the accuracy was almost 100%. The method presented the recovery ratio of 90-110% with modification in pretreatment method. The cross-validation of coefficient variation was 5 or less among tested three institutions in Korea. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=analytic%20method" title="analytic method">analytic method</a>, <a href="https://publications.waset.org/abstracts/search?q=canthaxanthin" title=" canthaxanthin"> canthaxanthin</a>, <a href="https://publications.waset.org/abstracts/search?q=food%20colorants" title=" food colorants"> food colorants</a>, <a href="https://publications.waset.org/abstracts/search?q=pretreatment%20method" title=" pretreatment method"> pretreatment method</a> </p> <a href="https://publications.waset.org/abstracts/24563/the-comparison-and-optimization-of-the-analytic-method-for-canthaxanthin-food-colorants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24563.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">683</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28040</span> Development of Lipid Architectonics for Improving Efficacy and Ameliorating the Oral Bioavailability of Elvitegravir </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bushra%20Nabi">Bushra Nabi</a>, <a href="https://publications.waset.org/abstracts/search?q=Saleha%20Rehman"> Saleha Rehman</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanjula%20Baboota"> Sanjula Baboota</a>, <a href="https://publications.waset.org/abstracts/search?q=Javed%20Ali"> Javed Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aim: The objective of research undertaken is analytical method validation (HPLC method) of an anti-HIV drug Elvitegravir (EVG). Additionally carrying out the forced degradation studies of the drug under different stress conditions to determine its stability. It is envisaged in order to determine the suitable technique for drug estimation, which would be employed in further research. Furthermore, comparative pharmacokinetic profile of the drug from lipid architectonics and drug suspension would be obtained post oral administration. Method: Lipid Architectonics (LA) of EVR was formulated using probe sonication technique and optimized using QbD (Box-Behnken design). For the estimation of drug during further analysis HPLC method has been validation on the parameters (Linearity, Precision, Accuracy, Robustness) and Limit of Detection (LOD) and Limit of Quantification (LOQ) has been determined. Furthermore, HPLC quantification of forced degradation studies was carried out under different stress conditions (acid induced, base induced, oxidative, photolytic and thermal). For pharmacokinetic (PK) study, Albino Wistar rats were used weighing between 200-250g. Different formulations were given per oral route, and blood was collected at designated time intervals. A plasma concentration profile over time was plotted from which the following parameters were determined: <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=AIDS" title="AIDS">AIDS</a>, <a href="https://publications.waset.org/abstracts/search?q=Elvitegravir" title=" Elvitegravir"> Elvitegravir</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=nanostructured%20lipid%20carriers" title=" nanostructured lipid carriers"> nanostructured lipid carriers</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmacokinetics" title=" pharmacokinetics"> pharmacokinetics</a> </p> <a href="https://publications.waset.org/abstracts/115553/development-of-lipid-architectonics-for-improving-efficacy-and-ameliorating-the-oral-bioavailability-of-elvitegravir" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/115553.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28039</span> Parameters of Validation Method of Determining Polycyclic Aromatic Hydrocarbons in Drinking Water by High Performance Liquid Chromatography</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jonida%20Canaj">Jonida Canaj</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A simple method of extraction and determination of fifteen priority polycyclic aromatic hydrocarbons (PAHs) from drinking water using high performance liquid chromatography (HPLC) has been validated with limits of detection (LOD) and limits of quantification (LOQ), method recovery and reproducibility, and other factors. HPLC parameters, such as mobile phase composition and flow standardized for determination of PAHs using fluorescent detector (FLD). PAH was carried out by liquid-liquid extraction using dichloromethane. Linearity of calibration curves was good for all PAH (R², 0.9954-1.0000) in the concentration range 0.1-100 ppb. Analysis of standard spiked water samples resulted in good recoveries between 78.5-150%(0.1ppb) and 93.04-137.47% (10ppb). The estimated LOD and LOQ ranged between 0.0018-0.98 ppb. The method described has been used for determination of the fifteen PAHs contents in drinking water samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=high%20performance%20liquid%20chromatography" title="high performance liquid chromatography">high performance liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=method%20validation" title=" method validation"> method validation</a>, <a href="https://publications.waset.org/abstracts/search?q=polycyclic%20aromatic%20hydrocarbons" title=" polycyclic aromatic hydrocarbons"> polycyclic aromatic hydrocarbons</a>, <a href="https://publications.waset.org/abstracts/search?q=PAHs" title=" PAHs"> PAHs</a>, <a href="https://publications.waset.org/abstracts/search?q=water" title=" water"> water</a> </p> <a href="https://publications.waset.org/abstracts/131378/parameters-of-validation-method-of-determining-polycyclic-aromatic-hydrocarbons-in-drinking-water-by-high-performance-liquid-chromatography" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/131378.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28038</span> Comparative Analysis of Glycated Hemoglobin (hba1c) Between HPLC and Immunoturbidimetry Method in Type II Diabetes Mellitus Patient</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Intanri%20Kurniati">Intanri Kurniati</a>, <a href="https://publications.waset.org/abstracts/search?q=Raja%20Iqbal%20Mulya%20Harahap"> Raja Iqbal Mulya Harahap</a>, <a href="https://publications.waset.org/abstracts/search?q=Agustyas%20Tjiptaningrum"> Agustyas Tjiptaningrum</a>, <a href="https://publications.waset.org/abstracts/search?q=Reni%20Zuraida"> Reni Zuraida</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Diabetes mellitus is still increasing and has become a health and social burden in the world. It is known that glycation among various proteins is increased in diabetic patients compared with non-diabetic subjects. Some of these glycated proteins are suggested to be involved in the development and progression of chronic diabetic complications. Among these glycated proteins, glycated hemoglobin (HbA1C) is commonly used as the gold standard index of glycemic control in the clinical setting. HbA1C testing has some methods, and the most commonly used is immunoturbidimetry. This research aimed to compare the HbA1c level between immunoturbidimetry and HbA1C level in T2DM patients. Methods: This research involves 77 patients from Abd Muluk Hospital Bandar Lampung; the patient was asked for consent in this research, then underwent phlebotomy to be tested for HbA1C; the sample was then examined for HbA1C with Turbidimetric Inhibition Immunoassay (TINIA) and High-Performance Liquid Chromatography (HPLC) method. Result: Mean± SD of the samples with the TINIA method was 9.2±1,2; meanwhile, the level HbA1C with the HPLC method is 9.6±1,2. The t-test showed no significant difference between the group subjects. (p<0.05). It was proposed that the two methods have high suitability in testing, and both are eligibly used for the patient. Discussion: There was no significant difference among research subjects, indicating that the high conformity of the two methods is suitable to be used for monitoring patients clinically. Conclusion: There is increasing in HbA1C level in a patient with T2DM measured with HPLC and or Turbidimetric Inhibition Immunoassay (TINIA) method, and there were no significant differences among those methods. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetes%20mellitus" title="diabetes mellitus">diabetes mellitus</a>, <a href="https://publications.waset.org/abstracts/search?q=glycated%20albumin" title=" glycated albumin"> glycated albumin</a>, <a href="https://publications.waset.org/abstracts/search?q=HbA1C" title=" HbA1C"> HbA1C</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=immunoturbidimetry" title=" immunoturbidimetry"> immunoturbidimetry</a> </p> <a href="https://publications.waset.org/abstracts/164008/comparative-analysis-of-glycated-hemoglobin-hba1c-between-hplc-and-immunoturbidimetry-method-in-type-ii-diabetes-mellitus-patient" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164008.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">99</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28037</span> A Method for Quantifying Arsenolipids in Sea Water by HPLC-High Resolution Mass Spectrometry </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muslim%20Khan">Muslim Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Kenneth%20B.%20Jensen"> Kenneth B. Jensen</a>, <a href="https://publications.waset.org/abstracts/search?q=Kevin%20A.%20Francesconi"> Kevin A. Francesconi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Trace amounts (ca 1 µg/L, 13 nM) of arsenic are present in sea water mostly as the oxyanion arsenate. In contrast, arsenic is present in marine biota (animals and algae) at very high levels (up to100,000 µg/kg) a significant portion of which is present as lipid-soluble compounds collectively termed arsenolipids. The complex nature of sea water presents an analytical challenge to detect trace compounds and monitor their environmental path. We developed a simple method using liquid-liquid extraction combined with HPLC-High Resolution Mass Spectrometer capable of detecting trace of arsenolipids (99 % of the sample matrix while recovering > 80 % of the six target arsenolipids with limit of detection of 0.003 µg/L.) <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=arsenolipids" title="arsenolipids">arsenolipids</a>, <a href="https://publications.waset.org/abstracts/search?q=sea%20water" title=" sea water"> sea water</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC-high%20resolution%20mass%20spectrometry" title=" HPLC-high resolution mass spectrometry"> HPLC-high resolution mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/39793/a-method-for-quantifying-arsenolipids-in-sea-water-by-hplc-high-resolution-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39793.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">366</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28036</span> Quantitative Analysis of (+)-Catechin and (-)-Epicatechin in Pentace burmanica Stem Bark by HPLC</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thidarat%20Duangyod">Thidarat Duangyod</a>, <a href="https://publications.waset.org/abstracts/search?q=Chanida%20Palanuvej"> Chanida Palanuvej</a>, <a href="https://publications.waset.org/abstracts/search?q=Nijsiri%20Ruangrungsi"> Nijsiri Ruangrungsi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pentace burmanica Kurz., belonging to the Malvaceae family, is commonly used for anti-diarrhea in Thai traditional medicine. A method for quantification of (+)-catechin and (-)-epicatechin in P. burmanica stem bark from 12 different Thailand markets by reverse-phase high performance liquid chromatography (HPLC) was investigated and validated. The analysis was performed by a Shimadzu DGU-20A3 HPLC equipped with a Shimadzu SPD-M20A photo diode array detector. The separation was accomplished with an Inersil ODS-3 column (5 µm x 4.6 x 250 mm) using 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) as mobile phase at the flow rate of 1 ml/min. The isocratic was set at 20% B for 15 min and the column temperature was maintained at 40 ºC. The detection was at the wavelength of 280 nm. Both (+)-catechin and (-)-epicatechin existed in the ethanolic extract of P. burmanica stem bark. The content of (-)-epicatechin was found as 59.74 ± 1.69 µg/mg of crude extract. In contrast, the quantitation of (+)-catechin content was omitted because of its small amount. The method was linear over a range of 5-200 µg/ml with good coefficients (r2 > 0.99) for (+)-catechin and (-)-epicatechin. Limit of detection values were found to be 4.80 µg/ml for (+)-catechin and 5.14 µg/ml for (-)-epicatechin. Limit of quantitation of (+)-catechin and (-)-epicatechin were of 14.54 µg/ml and 15.57 µg/ml respectively. Good repeatability and intermediate precision (%RSD < 3) were found in this study. The average recoveries of both (+)-catechin and (-)-epicatechin were obtained with good recovery in the range of 91.11 – 97.02% and 88.53 – 93.78%, respectively, with the %RSD less than 2. The peak purity indices of catechins were more than 0.99. The results suggested that HPLC method proved to be precise and accurate and the method can be conveniently used for (+)-catechin and (-)-epicatechin determination in ethanolic extract of P. burmanica stem bark. Moreover, the stem bark of P. burmanica was found to be a rich source of (-)-epicatechin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pentace%20burmanica" title="pentace burmanica">pentace burmanica</a>, <a href="https://publications.waset.org/abstracts/search?q=%28%2B%29-catechin" title=" (+)-catechin"> (+)-catechin</a>, <a href="https://publications.waset.org/abstracts/search?q=%28-%29-epicatechin" title=" (-)-epicatechin"> (-)-epicatechin</a>, <a href="https://publications.waset.org/abstracts/search?q=high%20performance%20liquid%20chromatography" title=" high performance liquid chromatography "> high performance liquid chromatography </a> </p> <a href="https://publications.waset.org/abstracts/19773/quantitative-analysis-of-catechin-and-epicatechin-in-pentace-burmanica-stem-bark-by-hplc" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19773.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">454</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28035</span> Determination of MDA by HPLC in Blood of Levofloxacin Treated Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20S.%20Mohale">D. S. Mohale</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20P.%20Dewani"> A. P. Dewani</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20S.tripathi"> A. S.tripathi</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20V.%20Chandewar"> A. V. Chandewar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Present work demonstrates the applicability of high-performance liquid chromatography (HPLC) with UV-Vis detection for the quantification of malondialdehyde as malondialdehyde-thiobarbituric acid complex (MDA-TBA) in-vivo in rats. The HPLC method for MDA-TBA was achieved by isocratic mode on a reverse-phase C18 column (250mm×4.6mm) at a flow rate of 1.0mLmin−1 followed by detection at 532 nm. The chromatographic conditions were optimized by varying the concentration and pH of water followed by changes in percentage of organic phase optimal mobile phase consisted of mixture of water (0.2% triethylamine pH adjusted to 2.3 by ortho-phosphoric acid) and acetonitrile in ratio (80:20v/v). The retention time of MDA-TBA complex was 3.7 min. The developed method was sensitive as limit of detection and quantification (LOD and LOQ) for MDA-TBA complex were (standard deviation and slope of calibration curve) 110 ng/ml and 363 ng/ml respectively. Calibration studies were done by spiking MDA into rat plasma at concentrations ranging from 500 to 1000 ng/ml. The precision of developed method measured in terms of relative standard deviations for intra-day and inter-day studies was 1.6–5.0% and 1.9–3.6% respectively. The HPLC method was applied for monitoring MDA levels in rats subjected to chronic treatment of levofloxacin (LEV) (5mg/kg/day) for 21 days. Results were compared by findings in control group rats. Mean peak areas of both study groups was subjected for statistical treatment to unpaired student t-test to find p-values. The p value was <0.001 indicating significant results and suggesting increased MDA levels in rats subjected to chronic treatment of LEV of 21 days. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=malondialdehyde-thiobarbituric%20acid%20complex" title="malondialdehyde-thiobarbituric acid complex">malondialdehyde-thiobarbituric acid complex</a>, <a href="https://publications.waset.org/abstracts/search?q=levofloxacin" title=" levofloxacin"> levofloxacin</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a> </p> <a href="https://publications.waset.org/abstracts/40000/determination-of-mda-by-hplc-in-blood-of-levofloxacin-treated-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40000.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">334</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28034</span> Rooting Out Breast Cancer by Repressing ER Gene Expression: Correlating Bioactivity of Pomegranate Rind with Chemical Constituents Identified by HPLC-MS/MS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alaa%20M.%20M.%20Badr%20Eldin">Alaa M. M. Badr Eldin</a>, <a href="https://publications.waset.org/abstracts/search?q=Marwa%20I.%20Ezzat"> Marwa I. Ezzat</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20S.%20Sedeek"> Mohammed S. Sedeek</a>, <a href="https://publications.waset.org/abstracts/search?q=Manal%20S.%20Afifi"> Manal S. Afifi</a>, <a href="https://publications.waset.org/abstracts/search?q=Omar%20M.%20Sabry"> Omar M. Sabry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cytotoxic activity of the total methanol extract against breast cancer cell line MCF-7 was amazing IC50 at 54 ug/ml. 130 polyphenolic compounds were tentatively identified in pomegranate peel (Punica granatum L.) methanol extract using HPLC-MS/MS technique. The antiestrogenic activity of the polyphenolic constituents found in pomegranate extract was confirmed experimentally in-vitro and by the in-silico molecular docking using gallagic acid, ellagic acid, and Punicalagin as these are considered model compounds confirmed in pomegranate peel extract. The methanolic extract was found to suppress ER, TGF-β, and NF-kB in-vitro gene expression strongly, and that was verified by qPCR and Western Blot gel electrophoresis techniques. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=HPLC-MS%2FMS" title="HPLC-MS/MS">HPLC-MS/MS</a>, <a href="https://publications.waset.org/abstracts/search?q=pomegranate" title=" pomegranate"> pomegranate</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title=" breast cancer"> breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=ovarian%20cancer" title=" ovarian cancer"> ovarian cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=ER" title=" ER"> ER</a>, <a href="https://publications.waset.org/abstracts/search?q=TGF-%CE%B2" title=" TGF-β"> TGF-β</a>, <a href="https://publications.waset.org/abstracts/search?q=NF-kB" title=" NF-kB"> NF-kB</a> </p> <a href="https://publications.waset.org/abstracts/158003/rooting-out-breast-cancer-by-repressing-er-gene-expression-correlating-bioactivity-of-pomegranate-rind-with-chemical-constituents-identified-by-hplc-msms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/158003.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">102</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28033</span> Characterization of Penicillin V Acid and Its Related Compounds by HPLC</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bahdja%20Guerfi">Bahdja Guerfi</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Hadhoum"> N. Hadhoum</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Azouz"> I. Azouz</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Bendoumia"> M. Bendoumia</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Bouafia"> S. Bouafia</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Z.%20Hadjadj%20Aoul"> F. Z. Hadjadj Aoul</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: 'Penicillin V' is a narrow, bactericidal antibiotic of the beta-lactam family of the naturally occurring penicillin group. It is limited to infections due to the germs defined as sensitive. The objective of this work was to identify and to characterize Penicillin V acid and its related compounds by High-performance liquid chromatography (HPLC). Methods: Firstly phenoxymethylpenicillin was identified by an infrared absorption. The organoleptic characteristics, pH, and determination of water content were also studied. The dosage of Penicillin V acid active substance and the determination of its related compounds were carried on waters HPLC, equipped with a UV detector at 254 nm and Discovery HS C18 column (250 mm X 4.6 mm X 5 µm) which is maintained at room temperature. The flow rate was about 1 ml per min. A mixture of water, acetonitrile and acetic acid (65:35:01) was used as mobile phase for phenoxyacetic acid ‘impurity B' and a mixture of water, acetonitrile and acetic acid (650:150:5.75) for the assay and 4-hydroxypenicillin V 'impurity D'. Results: The identification of Penicillin V acid active substance and the evaluation of its chemical quality showed conformity with USP 35th edition. The Penicillin V acid content in the raw material is equal to 1692.22 UI/mg. The percentage content of phenoxyacetic acid and 4-hydroxypenicillin V was respectively: 0.035% and 0.323%. Conclusion: Through these results, we can conclude that the Penicillin V acid active substance tested is of good physicochemical quality. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=characterization" title="characterization">characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=Penicillin%20V%20acid" title=" Penicillin V acid"> Penicillin V acid</a>, <a href="https://publications.waset.org/abstracts/search?q=related%20substances" title=" related substances"> related substances</a> </p> <a href="https://publications.waset.org/abstracts/76828/characterization-of-penicillin-v-acid-and-its-related-compounds-by-hplc" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76828.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">277</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28032</span> Nematicidal Activity of the Cell Extract from Penicillium Sp EU0013 and Its Metabolite Profile Using High Performance Liquid Chromatograpy </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zafar%20Iqbal">Zafar Iqbal</a>, <a href="https://publications.waset.org/abstracts/search?q=Sana%20Irshad%20Khan"> Sana Irshad Khan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organic extract from newly isolated plant growth promoting fungus (PGPF) Penicillium sp EU0013 was subjected to bioassays including anti fungal (disc diffusion) cytotoxicity (brine shrimp lethality), herbicidal (Lemna minor) and nematicidal activities. Metabolite profile of the extract was also assessed using HPLC analysis with the aim to identify bioactive natural products in the extract as new drug candidate(s). The extract showed anti fungal potential against tested fungal pathogens. Growth of the Wilt pathogen Fusarium oxyosproum was inhibited up to 63% when compared to negative reference. Activity against brine shrimps was weak and mortality up to 10% was observed at concentration of 200 µg. mL-1. The extract exhibited no toxicity against Lemna minor frond at 200 µg. mL-1. Nematicidal activity was observed very potent against root knot nematode and LC50 value was calculated as 52.5 ug. mL-1 using probit analysis. Methodically assessment of metabolites profile by HPLC showed the presence of kojic acid (Rt 1.4 min) and aflatoxin B1 (Rt 5.9 min) in the mycellial extract as compared with standards. The major unidentified metabolite was eluted at Rt 8.6 along with other minor peaks. The observed high toxicity against root knot nematode was attributed to the unidentified compounds that make fungal extract worthy of further exploration for isolation and structural characterization studies for development of future commercial nematicidal compound(s). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=penicillium" title="penicillium">penicillium</a>, <a href="https://publications.waset.org/abstracts/search?q=nematicidal%20activity" title=" nematicidal activity"> nematicidal activity</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolites" title=" metabolites"> metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a> </p> <a href="https://publications.waset.org/abstracts/19870/nematicidal-activity-of-the-cell-extract-from-penicillium-sp-eu0013-and-its-metabolite-profile-using-high-performance-liquid-chromatograpy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19870.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">446</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28031</span> Triggering Apoptosis to Uproot Breast Cancer: HPLC-MS/MS Profiling, in-vitro and in-silico Fascinating Results of Polyphenolics in Pomegranate Rind Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alaa%20M.%20Badr%20Eldin">Alaa M. Badr Eldin</a>, <a href="https://publications.waset.org/abstracts/search?q=Mayar%20M.%20Shahen"> Mayar M. Shahen</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20S.%20Sedeek"> Mohammed S. Sedeek</a>, <a href="https://publications.waset.org/abstracts/search?q=Marwa%20I.%20Ezzat"> Marwa I. Ezzat</a>, <a href="https://publications.waset.org/abstracts/search?q=Sawsan%20M.%20ElSonbaty"> Sawsan M. ElSonbaty</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammed%20A.%20Saad"> Muhammed A. Saad</a>, <a href="https://publications.waset.org/abstracts/search?q=Manal%20S.%20Afifi"> Manal S. Afifi</a>, <a href="https://publications.waset.org/abstracts/search?q=Omar%20M.%20Sabry"> Omar M. Sabry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Using HPLC-MS/MS technique, 133 polyphenolic compounds were identified in the methanol extract of pomegranate rind (Punica granatum L.). In-vitro cytotoxic activity against breast cancer cell line MCF-7 was investigated, with an IC50 of 54 ug/ml. In-silico molecular docking using ellagic acid, gallagic acid, and Punicalagin as model compounds identified in pomegranate rind extract confirmed the intriguing anti-estrogenic action of the key polyphenolic components in pomegranate rind extract. Surprisingly, taxol showed low activity compared to pomegranate compounds as ERα antagonist and ERβ agonist. Pomegranate rind extract enhanced apoptosis of breast cancer cells through upregulation of the caspase-3 expression and downregulation of NF-κB transcription factor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=HPLC-MS%2FMS" title="HPLC-MS/MS">HPLC-MS/MS</a>, <a href="https://publications.waset.org/abstracts/search?q=pomegranate%20rind" title=" pomegranate rind"> pomegranate rind</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=MCF-7" title=" MCF-7"> MCF-7</a>, <a href="https://publications.waset.org/abstracts/search?q=ER" title=" ER"> ER</a>, <a href="https://publications.waset.org/abstracts/search?q=caspase-3" title=" caspase-3"> caspase-3</a>, <a href="https://publications.waset.org/abstracts/search?q=NF-kB" title=" NF-kB"> NF-kB</a> </p> <a href="https://publications.waset.org/abstracts/163413/triggering-apoptosis-to-uproot-breast-cancer-hplc-msms-profiling-in-vitro-and-in-silico-fascinating-results-of-polyphenolics-in-pomegranate-rind-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163413.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">116</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28030</span> Metal (Loids) Speciation Using HPLC-ICP-MS Technique in Klodnica River, Upper Silesia, Poland</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Magdalena%20Jab%C5%82o%C5%84ska-Czapla">Magdalena Jabłońska-Czapla</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The work allowed gaining knowledge about redox and speciation changes of As, Cr, and Sb ionic forms in Klodnica River water. This kind of studies never has been conducted in this region of Poland. In study optimized and validated previously HPLC-ICP-MS methods for determination of As, Sb and Cr was used. Separation step was done using high-performance liquid chromatograph equipped with ion-exchange column followed by ICP-MS spectrometer detector. Preliminary studies included determination of the total concentration of As, Sb and Cr, pH, Eh, temperature and conductivity of the water samples. The study was conducted monthly from March to August 2014, at six points on the Klodnica River. The results indicate that exceeded at acceptable concentration of total Cr and Sb was observed in Klodnica River and we should qualify Klodnica River waters below the second purity class. In Klodnica River waters dominates oxidized antimony and arsenic forms, as well as the two forms of chromium Cr(VI) and Cr(III). Studies have also shown the methyl derivative of arsenic's presence. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimony" title="antimony">antimony</a>, <a href="https://publications.waset.org/abstracts/search?q=arsenic" title=" arsenic"> arsenic</a>, <a href="https://publications.waset.org/abstracts/search?q=chromium" title=" chromium"> chromium</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC-ICP-MS" title=" HPLC-ICP-MS"> HPLC-ICP-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=river%20water" title=" river water"> river water</a>, <a href="https://publications.waset.org/abstracts/search?q=speciation" title=" speciation"> speciation</a> </p> <a href="https://publications.waset.org/abstracts/17250/metal-loids-speciation-using-hplc-icp-ms-technique-in-klodnica-river-upper-silesia-poland" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17250.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">411</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28029</span> Comprehensive Validation of High-Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) for Quantitative Assessment of Caffeic Acid in Phenolic Extracts from Olive Mill Wastewater</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Layla%20El%20Gaini">Layla El Gaini</a>, <a href="https://publications.waset.org/abstracts/search?q=Majdouline%20Belaqziz"> Majdouline Belaqziz</a>, <a href="https://publications.waset.org/abstracts/search?q=Meriem%20Outaki"> Meriem Outaki</a>, <a href="https://publications.waset.org/abstracts/search?q=Mariam%20Minhaj"> Mariam Minhaj</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, it introduce and validate a high-performance liquid chromatography method with diode-array detection (HPLC-DAD) specifically designed for the accurate quantification of caffeic acid in phenolic extracts obtained from olive mill wastewater. The separation process of caffeic acid was effectively achieved through the use of an Acclaim Polar Advantage column (5µm, 250x4.6mm). A meticulous multi-step gradient mobile phase was employed, comprising water acidified with phosphoric acid (pH 2.3) and acetonitrile, to ensure optimal separation. The diode-array detection was adeptly conducted within the UV–VIS spectrum, spanning a range of 200–800 nm, which facilitated precise analytical results. The method underwent comprehensive validation, addressing several essential analytical parameters, including specificity, repeatability, linearity, as well as the limits of detection and quantification, alongside measurement uncertainty. The generated linear standard curves displayed high correlation coefficients, underscoring the method's efficacy and consistency. This validated approach is not only robust but also demonstrates exceptional reliability for the focused analysis of caffeic acid within the intricate matrices of wastewater, thus offering significant potential for applications in environmental and analytical chemistry. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=high-performance%20liquid%20chromatography%20%28HPLC-DAD%29" title="high-performance liquid chromatography (HPLC-DAD)">high-performance liquid chromatography (HPLC-DAD)</a>, <a href="https://publications.waset.org/abstracts/search?q=caffeic%20acid%20analysis" title=" caffeic acid analysis"> caffeic acid analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=olive%20mill%20wastewater%20phenolics" title=" olive mill wastewater phenolics"> olive mill wastewater phenolics</a>, <a href="https://publications.waset.org/abstracts/search?q=analytical%20method%20validation" title=" analytical method validation"> analytical method validation</a> </p> <a href="https://publications.waset.org/abstracts/179112/comprehensive-validation-of-high-performance-liquid-chromatography-diode-array-detection-hplc-dad-for-quantitative-assessment-of-caffeic-acid-in-phenolic-extracts-from-olive-mill-wastewater" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179112.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">70</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28028</span> RP-HPLC Method Development and Its Validation for Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil Combination in Bulk and Tablet Dosage Form</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Jain">S. Jain</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Savalia"> R. Savalia</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Saini"> V. Saini </a> </p> <p class="card-text"><strong>Abstract:</strong></p> A simple, accurate, precise, sensitive and specific RP-HPLC method was developed and validated for simultaneous estimation of Metoprolol Succinate and Olmesartan Medoxomil in bulk and tablet dosage form. The RP-HPLC method has shown adequate separation for Metoprolol Succinate and Olmesartan Medoxomil from its degradation products. The separation was achieved on a Phenomenex luna ODS C18 (250mm X 4.6mm i.d., 5μm particle size) with an isocratic mixture of acetonitrile: 50mM phosphate buffer pH 4.0 adjusted with glacial acetic acid in the ratio of 55:45 v/v. The mobile phase at a flow rate of 1.0ml/min, Injection volume 20μl and wavelength of detection was kept at 225nm. The retention time for Metoprolol Succinate and Olmesartan Medoxomil was 2.451±0.1min and 6.167±0.1min, respectively. The linearity of the proposed method was investigated in the range of 5-50μg/ml and 2-20μg/ml for Metoprolol Succinate and Olmesartan Medoxomil, respectively. Correlation coefficient was 0.999 and 0.9996 for Metoprolol Succinate and Olmesartan Medoxomil, respectively. The limit of detection was 0.2847μg/ml and 0.1251μg/ml for Metoprolol Succinate and Olmesartan Medoxomil, respectively and the limit of quantification was 0.8630μg/ml and 0.3793μg/ml for Metoprolol and Olmesartan, respectively. Proposed methods were validated as per ICH guidelines for linearity, accuracy, precision, specificity and robustness for estimation of Metoprolol Succinate and Olmesartan Medoxomil in commercially available tablet dosage form and results were found to be satisfactory. Thus the developed and validated stability indicating method can be used successfully for marketed formulations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=metoprolol%20succinate" title="metoprolol succinate">metoprolol succinate</a>, <a href="https://publications.waset.org/abstracts/search?q=olmesartan%20medoxomil" title=" olmesartan medoxomil"> olmesartan medoxomil</a>, <a href="https://publications.waset.org/abstracts/search?q=RP-HPLC%20method" title=" RP-HPLC method"> RP-HPLC method</a>, <a href="https://publications.waset.org/abstracts/search?q=validation" title=" validation"> validation</a>, <a href="https://publications.waset.org/abstracts/search?q=ICH" title=" ICH"> ICH</a> </p> <a href="https://publications.waset.org/abstracts/15356/rp-hplc-method-development-and-its-validation-for-simultaneous-estimation-of-metoprolol-succinate-and-olmesartan-medoxomil-combination-in-bulk-and-tablet-dosage-form" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15356.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28027</span> Evaluation of Polyphenolics Compounds in Cold Brewed Indian Tea</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chandrima%20Das">Chandrima Das</a>, <a href="https://publications.waset.org/abstracts/search?q=Sirshendu%20Chatterjee"> Sirshendu Chatterjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Tea (Camellia sinensis) is known as nature's low calorie wonder drink. Since ancient times hot consumptions of tea is very much popular. We have observed that many heat sensitive secondary metabolites which get destroyed on heating, moreover by people, who are permanently live at higher altitude or the members of high altitude expedition team, are deprived of various tea brewing facilities like electricity, fuel, etc. and the hence cold decoction of tea might be a good alternative. In this backdrop present study aims at the analysis of antioxidants like polyphenols, flavonoids and free radical scavenging activity as well as the l-theanine concentration of different types of cold brewed teas like black, green, white and oolong and compared with its hot decoction. Further, we also analysed in details about the bioactive components by using HPLC followed by green synthesis of nanoparticles. The study highlighted that the difference between the concentration of antioxidant in cold and hot brewed tea is insignificant and hence intake of cold decoction will be beneficial to health. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidants" title="antioxidants">antioxidants</a>, <a href="https://publications.waset.org/abstracts/search?q=flavanoid" title=" flavanoid"> flavanoid</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title=" polyphenols"> polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a> </p> <a href="https://publications.waset.org/abstracts/87647/evaluation-of-polyphenolics-compounds-in-cold-brewed-indian-tea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87647.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">306</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28026</span> Pharmacokinetic Study of Clarithromycin in Human Female of Pakistani Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Atifa%20Mushtaq">Atifa Mushtaq</a>, <a href="https://publications.waset.org/abstracts/search?q=Tanweer%20Khaliq"> Tanweer Khaliq</a>, <a href="https://publications.waset.org/abstracts/search?q=Hafiz%20Alam%20Sher"> Hafiz Alam Sher</a>, <a href="https://publications.waset.org/abstracts/search?q=Asia%20Farid"> Asia Farid</a>, <a href="https://publications.waset.org/abstracts/search?q=Anila%20Kanwal"> Anila Kanwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Maliha%20Sarfraz"> Maliha Sarfraz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study was designed to assess the various pharmacokinetic parameters of a commercially available clarithromycin Tablet (Klaricid® 250 mg Abbot, Pakistan) in plasma sample of healthy adult female volunteers by applying a rapid, sensitive and accurate HPLC-UV analytical method. The human plasma samples were evaluated by using an isocratic High Performance Liquid Chromatography (HPLC) system of Sykam consisted of a pump with a column C18 column (250×4.6mn, 5µm) UV-detector. The mobile phase comprises of potassium dihydrogen phosphate (50 mM, pH 6.8, contained 0.7% triethylamine), methanol and acetonitrile (30:25:45, v/v/v) was delivered with injection volume of 20µL at flow rate of 1 mL/min. The detection was performed at λmax 275 nm. By applying this method, important pharmacokinetic parameters Cmax, Tmax, Area under curve (AUC), half-life (t1/2), , Volume of distribution (Vd) and Clearance (Cl) were measured. The parameters of pharmacokinetics of clarithromycin were calculated by software (APO) pharmacological analysis. Maximum plasma concentrations Cmax 2.78 ±0.33 µg/ml, time to reach maximum concentration tmax 2.82 ± 0.11 h and Area under curve AUC was 20.14 h.µg/ml. The mean ± SD values obtained for the pharmacokinetic parameters showed a significant difference in pharmacokinetic parameters observed in previous literature which emphasizes the need for dose adjustment of clarithromycin in Pakistani population. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pharmacokinetc" title="Pharmacokinetc">Pharmacokinetc</a>, <a href="https://publications.waset.org/abstracts/search?q=Clarothromycin" title="Clarothromycin">Clarothromycin</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title="HPLC">HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=Pakistan" title="Pakistan">Pakistan</a> </p> <a href="https://publications.waset.org/abstracts/143201/pharmacokinetic-study-of-clarithromycin-in-human-female-of-pakistani-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143201.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">108</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28025</span> Chromatographic Lipophilicity Determination of Newly Synthesized Steroid Derivatives for Further Biological Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Milica%20Z.%20Karadzic">Milica Z. Karadzic</a>, <a href="https://publications.waset.org/abstracts/search?q=Lidija%20R.%20Jevric"> Lidija R. Jevric</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanja%20Podunavac-Kuzmanovic"> Sanja Podunavac-Kuzmanovic</a>, <a href="https://publications.waset.org/abstracts/search?q=Strahinja%20Z.%20Kovacevic"> Strahinja Z. Kovacevic</a>, <a href="https://publications.waset.org/abstracts/search?q=Anamarija%20I.%20Mandic"> Anamarija I. Mandic</a>, <a href="https://publications.waset.org/abstracts/search?q=Katarina%20Penov-Gasi"> Katarina Penov-Gasi</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrea%20R.%20Nikolic"> Andrea R. Nikolic</a>, <a href="https://publications.waset.org/abstracts/search?q=Aleksandar%20M.%20Okljesa"> Aleksandar M. Okljesa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, a set of 29 newly synthesized steroid derivatives were investigated using reversed-phase high-performance liquid chromatography (RP-HPLC) as a first step in preselection of drug candidates. This analysis presents an experimental determination of chromatographic lipophilicity, and it was conducted to obtain physicochemical characterization of these molecules. As the most widely used bonded phases in RP-HPLC, octadecyl (C18) and octyl (C8) were used. Binary mixtures of water and acetonitrile or methanol were used as mobile phases. Obtained results were expressed as retention factor values logk and they were correlated with logP values. The results showed that both columns provide good estimations of the chromatographic lipophilicity of the molecules included in this study. This analysis was conducted in order to characterize newly synthesized steroid derivatives for further investigation regarding their antiproliferative and antimicrobial activity. This article is based upon work from COST Action (CM1306), supported by COST (European Cooperation in Science and Technology). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antiproliferative%20activity" title="antiproliferative activity">antiproliferative activity</a>, <a href="https://publications.waset.org/abstracts/search?q=chromatographic%20lipophilicity" title=" chromatographic lipophilicity"> chromatographic lipophilicity</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid%20chromatography" title=" liquid chromatography"> liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=steroids" title=" steroids"> steroids</a> </p> <a href="https://publications.waset.org/abstracts/49454/chromatographic-lipophilicity-determination-of-newly-synthesized-steroid-derivatives-for-further-biological-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/49454.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">290</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28024</span> Quality by Design in the Optimization of a Fast HPLC Method for Quantification of Hydroxychloroquine Sulfate</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pedro%20J.%20Rolim-Neto">Pedro J. Rolim-Neto</a>, <a href="https://publications.waset.org/abstracts/search?q=Leslie%20R.%20M.%20Ferraz"> Leslie R. M. Ferraz</a>, <a href="https://publications.waset.org/abstracts/search?q=Fabiana%20L.%20A.%20Santos"> Fabiana L. A. Santos</a>, <a href="https://publications.waset.org/abstracts/search?q=Pablo%20A.%20Ferreira"> Pablo A. Ferreira</a>, <a href="https://publications.waset.org/abstracts/search?q=Ricardo%20T.%20L.%20Maia-Jr."> Ricardo T. L. Maia-Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Magaly%20A.%20M.%20Lyra"> Magaly A. M. Lyra</a>, <a href="https://publications.waset.org/abstracts/search?q=Danilo%20A%20F.%20Fonte"> Danilo A F. Fonte</a>, <a href="https://publications.waset.org/abstracts/search?q=Salvana%20P.%20M.%20Costa"> Salvana P. M. Costa</a>, <a href="https://publications.waset.org/abstracts/search?q=Amanda%20C.%20Q.%20M.%20Vieira"> Amanda C. Q. M. Vieira</a>, <a href="https://publications.waset.org/abstracts/search?q=Larissa%20A.%20Rolim"> Larissa A. Rolim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Initially developed as an antimalarial agent, hydroxychloroquine (HCQ) sulfate is often used as a slow-acting antirheumatic drug in the treatment of disorders of connective tissue. The United States Pharmacopeia (USP) 37 provides a reversed-phase HPLC method for quantification of HCQ. However, this method was not reproducible, producing asymmetric peaks in a long analysis time. The asymmetry of the peak may cause an incorrect calculation of the concentration of the sample. Furthermore, the analysis time is unacceptable, especially regarding the routine of a pharmaceutical industry. The aiming of this study was to develop a fast, easy and efficient method for quantification of HCQ sulfate by High Performance Liquid Chromatography (HPLC) based on the Quality by Design (QbD) methodology. This method was optimized in terms of peak symmetry using the surface area graphic as the Design of Experiments (DoE) and the tailing factor (TF) as an indicator to the Design Space (DS). The reference method used was that described at USP 37 to the quantification of the drug. For the optimized method, was proposed a 33 factorial design, based on the QbD concepts. The DS was created with the TF (in a range between 0.98 and 1.2) in order to demonstrate the ideal analytical conditions. Changes were made in the composition of the USP mobile-phase (USP-MP): USP-MP: Methanol (90:10 v/v, 80:20 v/v and 70:30 v/v), in the flow (0.8, 1.0 and 1.2 mL) and in the oven temperature (30, 35, and 40ºC). The USP method allowed the quantification of drug in a long time (40-50 minutes). In addition, the method uses a high flow rate (1,5 mL.min-1) which increases the consumption of expensive solvents HPLC grade. The main problem observed was the TF value (1,8) that would be accepted if the drug was not a racemic mixture, since the co-elution of the isomers can become an unreliable peak integration. Therefore, the optimization was suggested in order to reduce the analysis time, aiming a better peak resolution and TF. For the optimization method, by the analysis of the surface-response plot it was possible to confirm the ideal setting analytical condition: 45 °C, 0,8 mL.min-1 and 80:20 USP-MP: Methanol. The optimized HPLC method enabled the quantification of HCQ sulfate, with a peak of high resolution, showing a TF value of 1,17. This promotes good co-elution of isomers of the HCQ, ensuring an accurate quantification of the raw material as racemic mixture. This method also proved to be 18 times faster, approximately, compared to the reference method, using a lower flow rate, reducing even more the consumption of the solvents and, consequently, the analysis cost. Thus, an analytical method for the quantification of HCQ sulfate was optimized using QbD methodology. This method proved to be faster and more efficient than the USP method, regarding the retention time and, especially, the peak resolution. The higher resolution in the chromatogram peaks supports the implementation of the method for quantification of the drug as racemic mixture, not requiring the separation of isomers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=analytical%20method" title="analytical method">analytical method</a>, <a href="https://publications.waset.org/abstracts/search?q=hydroxychloroquine%20sulfate" title=" hydroxychloroquine sulfate"> hydroxychloroquine sulfate</a>, <a href="https://publications.waset.org/abstracts/search?q=quality%20by%20design" title=" quality by design"> quality by design</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20area%20graphic" title=" surface area graphic"> surface area graphic</a> </p> <a href="https://publications.waset.org/abstracts/25658/quality-by-design-in-the-optimization-of-a-fast-hplc-method-for-quantification-of-hydroxychloroquine-sulfate" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25658.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">639</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28023</span> Separation of Fexofenadine Enantiomers Using Beta Cyclodextrin as Chiral Counter Ion in Mobile Phase</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=R.%20Fegas">R. Fegas</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Zerkout"> S. Zerkout</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Taberkokt"> S. Taberkokt</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Righezza"> M. Righezza </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present work demonstrate the potential of Betacyclodextrine (BCD) for the chiral analysis of a drug .Various separation mechanisms were applied and several parameters affecting the separation were studied, including the type and concentration of chiral selector, and pH of buffer. A simple and sensitive high-performance liquid chromatography (HPLC) method was developed as an assay for fexofenadine enantiomers in pharmaceutical preparation. Fexofenadine enantiomers were separated using a mobile phase of 0.25mM NaH2PO4–acetonitrile (65:35, v/v) – Betacyclodextrine on achiral phenyl-urea column at a ﬂow rate of 1ml/min and measurement at 220nm. The chiral mechanism of separation was mainly based on specific interaction between the solute and the stationary phase. The retention was directly controlled by mobile phase composition but not the selectivity which results of the two mechanisms, electrostatic interactions and partition mechanism. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fexofenadine%20enantiomer" title="fexofenadine enantiomer">fexofenadine enantiomer</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC" title=" HPLC"> HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=achiral%20phenyl-urea%20column" title=" achiral phenyl-urea column"> achiral phenyl-urea column</a> </p> <a href="https://publications.waset.org/abstracts/12321/separation-of-fexofenadine-enantiomers-using-beta-cyclodextrin-as-chiral-counter-ion-in-mobile-phase" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12321.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">458</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28022</span> An Investigation of How Salad Rocket May Provide Its Own Defence Against Spoilage Bacteria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Huda%20Aldossari">Huda Aldossari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Members of the Brassicaceae family, such as rocket species, have high concentrations of glucosinolates (GLSs). GSLs and isothiocyanates (ITCs), the product of GLSs hydrolysis, are the most influential compounds that affect flavour in rocket species. Aside from their contribution to the flavour, GSLs and ITCs are of particular interest due to their potential ability to inhibit the growth of human pathogenic bacteria such as E. coli O157. Quantitative and qualitative analysis of glucosinolate compounds in rocket extracts was obtained by Liquid Chromatography-Mass Spectrometry (LC–MS).Each individual component of non-volatile GLSs and ITCs was isolated by High-Performance Liquid Chromatography (HPLC) fractionation. The identity and purity of each fraction were confirmed using Ultra High-Performance Liquid Chromatography (UPLC). The separation of glucosinolates in the complex rocket extractions was performed by optimizing a HPLC fractionation method through changing the mobile phase composition, solvent gradient, and the flow rate. As a result, six glucosinolates compounds (Glucosativin, 4-Methoxyglucobrassicin, Glucotropaeolin GTP, Glucoiberin GIB, Diglucothiobenin, and Sinigrin) have been isolated, identified and quantified in the complex samples. This step aims to evaluate the antibacterial activity of glucosinolates and their enzymatic hydrolysis against bacterial growth of E.coli k12. Therefore, fractions from this study will be used to determine the most active compounds by investigating the efficacy of each component of GLSs and ITCs at inhibiting bacterial growth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rocket" title="rocket">rocket</a>, <a href="https://publications.waset.org/abstracts/search?q=glucosinolates" title=" glucosinolates"> glucosinolates</a>, <a href="https://publications.waset.org/abstracts/search?q=E.coli%20k12." title=" E.coli k12."> E.coli k12.</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC%20fractionatio" title=" HPLC fractionatio"> HPLC fractionatio</a> </p> <a href="https://publications.waset.org/abstracts/158926/an-investigation-of-how-salad-rocket-may-provide-its-own-defence-against-spoilage-bacteria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/158926.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">96</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28021</span> Tracking of Linarin from the Ethyl Acetate Fraction of Melinjo (Gnetum gnemon L.) Seeds Using Preparative High Performance Liquid Chromatography</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asep%20Sukohar">Asep Sukohar</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramadhan%20Triyandi"> Ramadhan Triyandi</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Iqbal"> Muhammad Iqbal</a>, <a href="https://publications.waset.org/abstracts/search?q=Sahidin"> Sahidin</a>, <a href="https://publications.waset.org/abstracts/search?q=Suharyani"> Suharyani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Resveratrol is a class of bioactive chemicals found in melinjo, which has a wide range of biological actions. The purpose of this study is to determine the linarin content of the melinjo fraksi by using preparative-high-performance liquid chromatography (prep-HPLC). Method: Extraction used the soxhletation method with 96% ethanol solvent. Fractionation used ethyl acetate and ethanol in a ratio of 1:1. Tracing of linarin compound used prep-HPLC with a mobile phase ratio of distilled water: methanol (55: 45, v/v). The presence of linarin was detected using a wavelength of 215 nm. Fourier Transform Infrared (FTIR) was used to identify the functional groups of compound. Result: The retention time required to elute the ethyl acetate fraction was 2.601 minutes. Compound separation identification using Fourier Transform Infrared Spectroscopy - Quest Attenuated Total Reflectance (FTIR - QATR) has a similarity value range with standards from 0 to 1000. The elution results of the ethyl acetate fraction have similar values with the standard compounds linarin (668), resveratrol (578), and catechin (455). Conclusion: Tracing for active compound in the ethyl acetate fraction of Gnetum Gnemon L. using prep-HPLC showed a strong suspicion of the presence of linarin compound. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gnetum%20gnemon%20L." title="Gnetum gnemon L.">Gnetum gnemon L.</a>, <a href="https://publications.waset.org/abstracts/search?q=linarin" title=" linarin"> linarin</a>, <a href="https://publications.waset.org/abstracts/search?q=prep-HPLC" title=" prep-HPLC"> prep-HPLC</a>, <a href="https://publications.waset.org/abstracts/search?q=fraction%20ethyl%20acetate" title=" fraction ethyl acetate"> fraction ethyl acetate</a> </p> <a href="https://publications.waset.org/abstracts/171258/tracking-of-linarin-from-the-ethyl-acetate-fraction-of-melinjo-gnetum-gnemon-l-seeds-using-preparative-high-performance-liquid-chromatography" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171258.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">116</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=HPLC%20analysis&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=HPLC%20analysis&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=HPLC%20analysis&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=HPLC%20analysis&page=5">5</a></li> <li class="page-item"><a class="page-link" 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