CFTR Antibody (24-1) [Unconjugated] - C-terminus (MAB25031): Novus Biologicals

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View Mouse Monoclonal anti-CFTR Antibody (24-1) [Unconjugated] - C-terminus (MAB25031). Validated Applications: WB, IHC, IP. Validated Species: Human. Sample size available." /> <meta property="og:description" content="Cited in 76 publications. View Mouse Monoclonal anti-CFTR Antibody (24-1) [Unconjugated] - C-terminus (MAB25031). Validated Applications: WB, IHC, IP. Validated Species: Human. Sample size available." /> <meta property="og:image" content="//images.novusbio.com/thumbnails2/CFTR_MAB25031_Immunohistochemistry_6973.jpg" /> <meta name="robots" content="index,follow" /> <meta name="baidu-site-verification" content="xQr2vSIwwB" /> <script id="json-ld" type="application/ld+json">[{"@context":"http://schema.org","@type":"Product","description":"Mouse Monoclonal Anti-CFTR Antibody (24-1) [Unconjugated] - C-terminus. Tested Applications: Western Blot, IHC, IP. 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Half of the membrane was probed with anti-CFTR mAb and the other half with anti-ubiquitin mAb. Lysates were loaded onto an 11% SDS-PAGE and sequential probing of the membrane was performed (COMMD1, alpha -tubulin and lastly ubiquitin). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (B) Quantification of ubiquitinated CFTR. Ratio of ubiquitinated CFTR to total CFTR in each condition is shown, endogenous COMMD1 expression is referred as 100%. The means \u00b1 S.D. were obtained from five independent experiments.* P<0.05 was determined by t-test. (C) Stability of the mature wt-CFTR was determined upon inhibition of protein biosynthesis with cycloheximide (CHX). Cells were incubated in the presence of cycloheximide for the indicated time intervals. (D) Quantification of mature CFTR was normalized to alpha -tubulin level. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511329.jpg","caption":"Co-localisation by immunofluorescence of CFTR and actin. (A) The pattern of CFTR (green staining) and actin (red staining) stainings was essentially apical in control cells as well as in cells treated with Sal/FP (B). (C) The incubation of cells with S. aureus supernatant induced alteration of the localisation of CFTR that appeared to be cytoplasmic, in parallel with a disorganization of the actin network. (D) Treatment of S. aureus supernatant pre-incubated cells with Sal/FP restored CFTR and actin apical stainings. (E) Quantification of the co-localisation of CFTR and actin showed that 2% S. aureus supernatant decreased the co-localisation index compared to the index in control cells, but the difference was not significant; the treatment with Sal/FP alone or after S. aureus supernatant incubation significantly enhanced the co-localisation of the 2 proteins compared with control or with S. aureus supernatant-treated cells (*, p < 0.05). Data represent the mean \u00b1 SEM of 3 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg","caption":"COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 \u00b5g of anti-COMMD1 mAb (Abnova), 0.8 \u00b5g of anti-CFTR mAb (MAB25031, R&D Systems) or with 0.8 \u00b5g anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512671.jpg","caption":"Manganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24\u00a0hours. CFTR protein was detected by immunobloting using a monoclonal antibody as described in Materials and Methods. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511345.jpg","caption":"Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean \u00b1 SEM of 5 different experiments. 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(C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24\u00a0hours. Results are expressed as fold change and are representative of three independent experiments. *p\u2009<\u20090.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511315.jpg","caption":"Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a\u2013c; red) or Cftr (d\u2013f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. 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CFTR protein was detected by immunoblotting 48\u00a0hours after treatment. Blots are representative of at least three independent experiments. *p\u2009<\u20090.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513841.jpg","caption":"Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a\u2013c; red) or Cftr (d\u2013f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10\u00d7) of boxed areas in (a\u2013f) correspond to panels (a\u2032\u2013f\u2032). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 \u00b5m (a\u2013f), 10 \u00b5m (a\u2032\u2013c\u2032). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems.","width":400,"height":400},{"@type":"ImageObject","url":"//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512696.jpg","caption":"Immunohistochemical localization of transporters in anterior and posterior intestine of tilapia acclimated to different salinities.Representative micrographs of immunolocalization of Nka (green) with Nkcc/Ncc (red) (a\u2013d, f\u2013h) from FW (a, f), SW (b, g) and HSW (c, h) acclimated tilapia. (d) A representative higher magnification micrograph of Nkcc/Ncc staining of the brush border of enterocytes with basolateral Nka staining from the anterior intestine of SW-acclimated fish. (e) Apical Cftr (red) double labeling with Nka (green) in the anterior intestine of a FW-acclimated fish. Panels (a\u2013e) are sections of anterior intestine (AI) while panels (f\u2013h) are sections of posterior intestine (PI). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Scale bar 100 \u00b5m (a\u2013c, f\u2013h); 25 \u00b5m (d,e). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. 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5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>CFTR Antibody (24-1) [Unconjugated] - C-terminus</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(76 Publications)</span></a></div><div class="ds_info float_left"><a href="#ReviewsSection"><div class="dsSprite_stars4"></div></a> <a href="#ReviewsSection" class="bottom0"><span class="ctr">(10 Reviews)</span></a></div><div class="ds_info float_left"> <a href="/common-name/cftr"><div class="sprite_allRelated"></div></a> <a href="/common-name/cftr" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/MAB25031"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/MAB25031" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="5" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/CFTR_MAB25031_Immunohistochemistry_6973.jpg" class="top_images" rel="gallery" title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > <img src="//images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg" class="big_thumb" alt="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." /> </a> </div> <div class="alt-caption padding_left5" >CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/CFTR_MAB25031_Immunohistochemistry_9671.jpg" class="top_images" rel="gallery" title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > <img src="//images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." /> </a> </div> <div class="alt-caption padding_left5" >CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513847.jpg" class="top_images" rel="gallery" title="Chronic exposure to cigarette smoke (CS) decreases airway surface liquid (ASL) height. Primary human airway epithelial cells from 4 donors (n = 8) were exposed to 30 puffs of whole cigarette smoke (2 cigarettes) every day for 5 days (120 hrs). (A) ASL height was measured one hour after each exposure to CS. ASL height was undisturbed over the course of the reading. *p < 0.05. (B) CFTR present at the plasma membrane was detected by immunoblotting after biotinylation of cell surface proteins (see Methods). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513847.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Chronic exposure to cigarette smoke (CS) decreases airway surface liquid (ASL) height. Primary human airway epithelial cells from 4 donors (n = 8) were exposed to 30 puffs of whole cigarette smoke (2 cigarettes) every day for 5 days (120 hrs). (A) ASL height was measured one hour after each exposure to CS. ASL height was undisturbed over the course of the reading. *p < 0.05. (B) CFTR present at the plasma membrane was detected by immunoblotting after biotinylation of cell surface proteins (see Methods). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Chronic exposure to cigarette smoke (CS) decreases airway surface liquid (ASL) height. Primary human airway epithelial cells from 4 donors (n = 8) were exposed to 30 puffs of whole cigarette smoke (2 cigarettes) every day for 5 days (120 hrs). (A) ASL height was measured one hour after each exposure to CS. ASL height was undisturbed over the course of the reading. *p < 0.05. (B) CFTR present at the plasma membrane was detected by immunoblotting after biotinylation of cell surface proteins (see Methods). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Chronic exposure to cigarette smoke (CS) decreases airway surface liquid (ASL) height. Primary human airway epithelial cells from 4 donors (n = 8) were exposed to 30 puffs of whole cigarette smoke (2 cigarettes)<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511322.jpg" class="top_images" rel="gallery" title="Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511322.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we<a class="openGallery" href="#" data-picnum="3"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412514839.jpg" class="top_images" rel="gallery" title="Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412514839.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of<a class="openGallery" href="#" data-picnum="4"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-4120241251381.jpg" class="top_images" rel="gallery" title="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-4120241251381.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr<a class="openGallery" href="#" data-picnum="5"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512653.jpg" class="top_images" rel="gallery" title="COMMD1 regulates CFTR ubiquitination.(A) Representative gels for the same CFTR IP experiment with MAB25031 from HeLa cells stably expressing wt-CFTR and separated on 8% SDS-PAGE transferred to PVDF membrane. Half of the membrane was probed with anti-CFTR mAb and the other half with anti-ubiquitin mAb. Lysates were loaded onto an 11% SDS-PAGE and sequential probing of the membrane was performed (COMMD1, alpha -tubulin and lastly ubiquitin). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (B) Quantification of ubiquitinated CFTR. Ratio of ubiquitinated CFTR to total CFTR in each condition is shown, endogenous COMMD1 expression is referred as 100%. The means ± S.D. were obtained from five independent experiments.* P<0.05 was determined by t-test. (C) Stability of the mature wt-CFTR was determined upon inhibition of protein biosynthesis with cycloheximide (CHX). Cells were incubated in the presence of cycloheximide for the indicated time intervals. (D) Quantification of mature CFTR was normalized to alpha -tubulin level. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512653.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="COMMD1 regulates CFTR ubiquitination.(A) Representative gels for the same CFTR IP experiment with MAB25031 from HeLa cells stably expressing wt-CFTR and separated on 8% SDS-PAGE transferred to PVDF membrane. Half of the membrane was probed with anti-CFTR mAb and the other half with anti-ubiquitin mAb. Lysates were loaded onto an 11% SDS-PAGE and sequential probing of the membrane was performed (COMMD1, alpha -tubulin and lastly ubiquitin). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (B) Quantification of ubiquitinated CFTR. Ratio of ubiquitinated CFTR to total CFTR in each condition is shown, endogenous COMMD1 expression is referred as 100%. The means ± S.D. were obtained from five independent experiments.* P<0.05 was determined by t-test. (C) Stability of the mature wt-CFTR was determined upon inhibition of protein biosynthesis with cycloheximide (CHX). Cells were incubated in the presence of cycloheximide for the indicated time intervals. (D) Quantification of mature CFTR was normalized to alpha -tubulin level. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems." title="COMMD1 regulates CFTR ubiquitination.(A) Representative gels for the same CFTR IP experiment with MAB25031 from HeLa cells stably expressing wt-CFTR and separated on 8% SDS-PAGE transferred to PVDF membrane. Half of the membrane was probed with anti-CFTR mAb and the other half with anti-ubiquitin mAb. Lysates were loaded onto an 11% SDS-PAGE and sequential probing of the membrane was performed (COMMD1, alpha -tubulin and lastly ubiquitin). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (B) Quantification of ubiquitinated CFTR. Ratio of ubiquitinated CFTR to total CFTR in each condition is shown, endogenous COMMD1 expression is referred as 100%. The means ± S.D. were obtained from five independent experiments.* P<0.05 was determined by t-test. (C) Stability of the mature wt-CFTR was determined upon inhibition of protein biosynthesis with cycloheximide (CHX). Cells were incubated in the presence of cycloheximide for the indicated time intervals. (D) Quantification of mature CFTR was normalized to alpha -tubulin level. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >COMMD1 regulates CFTR ubiquitination.(A) Representative gels for the same CFTR IP experiment with MAB25031 from HeLa cells stably expressing wt-CFTR and separated on 8% SDS-PAGE transferred to PVDF membrane. Half of the<a class="openGallery" href="#" data-picnum="6"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511329.jpg" class="top_images" rel="gallery" title="Co-localisation by immunofluorescence of CFTR and actin. (A) The pattern of CFTR (green staining) and actin (red staining) stainings was essentially apical in control cells as well as in cells treated with Sal/FP (B). (C) The incubation of cells with S. aureus supernatant induced alteration of the localisation of CFTR that appeared to be cytoplasmic, in parallel with a disorganization of the actin network. (D) Treatment of S. aureus supernatant pre-incubated cells with Sal/FP restored CFTR and actin apical stainings. (E) Quantification of the co-localisation of CFTR and actin showed that 2% S. aureus supernatant decreased the co-localisation index compared to the index in control cells, but the difference was not significant; the treatment with Sal/FP alone or after S. aureus supernatant incubation significantly enhanced the co-localisation of the 2 proteins compared with control or with S. aureus supernatant-treated cells (*, p < 0.05). Data represent the mean ± SEM of 3 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511329.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Co-localisation by immunofluorescence of CFTR and actin. (A) The pattern of CFTR (green staining) and actin (red staining) stainings was essentially apical in control cells as well as in cells treated with Sal/FP (B). (C) The incubation of cells with S. aureus supernatant induced alteration of the localisation of CFTR that appeared to be cytoplasmic, in parallel with a disorganization of the actin network. (D) Treatment of S. aureus supernatant pre-incubated cells with Sal/FP restored CFTR and actin apical stainings. (E) Quantification of the co-localisation of CFTR and actin showed that 2% S. aureus supernatant decreased the co-localisation index compared to the index in control cells, but the difference was not significant; the treatment with Sal/FP alone or after S. aureus supernatant incubation significantly enhanced the co-localisation of the 2 proteins compared with control or with S. aureus supernatant-treated cells (*, p < 0.05). Data represent the mean ± SEM of 3 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Co-localisation by immunofluorescence of CFTR and actin. (A) The pattern of CFTR (green staining) and actin (red staining) stainings was essentially apical in control cells as well as in cells treated with Sal/FP (B). (C) The incubation of cells with S. aureus supernatant induced alteration of the localisation of CFTR that appeared to be cytoplasmic, in parallel with a disorganization of the actin network. (D) Treatment of S. aureus supernatant pre-incubated cells with Sal/FP restored CFTR and actin apical stainings. (E) Quantification of the co-localisation of CFTR and actin showed that 2% S. aureus supernatant decreased the co-localisation index compared to the index in control cells, but the difference was not significant; the treatment with Sal/FP alone or after S. aureus supernatant incubation significantly enhanced the co-localisation of the 2 proteins compared with control or with S. aureus supernatant-treated cells (*, p < 0.05). Data represent the mean ± SEM of 3 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Co-localisation by immunofluorescence of CFTR and actin. (A) The pattern of CFTR (green staining) and actin (red staining) stainings was essentially apical in control cells as well as in cells treated with Sal/FP (B).<a class="openGallery" href="#" data-picnum="7"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg" class="top_images" rel="gallery" title="COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 µg of anti-COMMD1 mAb (Abnova), 0.8 µg of anti-CFTR mAb (MAB25031, R&D Systems) or with 0.8 µg anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 µg of anti-COMMD1 mAb (Abnova), 0.8 µg of anti-CFTR mAb (MAB25031, R&D Systems) or with 0.8 µg anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems." title="COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 µg of anti-COMMD1 mAb (Abnova), 0.8 µg of anti-CFTR mAb (MAB25031, R&D Systems) or with 0.8 µg anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the<a class="openGallery" href="#" data-picnum="8"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512671.jpg" class="top_images" rel="gallery" title="Manganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24 hours. CFTR protein was detected by immunobloting using a monoclonal antibody as described in Materials and Methods. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512671.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Manganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24 hours. CFTR protein was detected by immunobloting using a monoclonal antibody as described in Materials and Methods. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Manganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24 hours. CFTR protein was detected by immunobloting using a monoclonal antibody as described in Materials and Methods. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Manganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24 hours. CFTR<a class="openGallery" href="#" data-picnum="9"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511345.jpg" class="top_images" rel="gallery" title="Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511345.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we<a class="openGallery" href="#" data-picnum="10"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412505357.jpg" class="top_images" rel="gallery" title="Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412505357.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of<a class="openGallery" href="#" data-picnum="11"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511315.jpg" class="top_images" rel="gallery" title="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412511315.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr<a class="openGallery" href="#" data-picnum="12"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412505379.jpg" class="top_images" rel="gallery" title="Metals present in CSE regulate CFTR expression. 16HBE14o- cells were incubated with 10% CSE before and after incubation with Chelex-100 beads, in absence or presence of 10 μM cadmium chloride. CFTR protein was detected by immunoblotting 48 hours after treatment. Blots are representative of at least three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412505379.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Metals present in CSE regulate CFTR expression. 16HBE14o- cells were incubated with 10% CSE before and after incubation with Chelex-100 beads, in absence or presence of 10 μM cadmium chloride. CFTR protein was detected by immunoblotting 48 hours after treatment. Blots are representative of at least three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Metals present in CSE regulate CFTR expression. 16HBE14o- cells were incubated with 10% CSE before and after incubation with Chelex-100 beads, in absence or presence of 10 μM cadmium chloride. CFTR protein was detected by immunoblotting 48 hours after treatment. Blots are representative of at least three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Metals present in CSE regulate CFTR expression. 16HBE14o- cells were incubated with 10% CSE before and after incubation with Chelex-100 beads, in absence or presence of 10 μM cadmium chloride. CFTR protein was<a class="openGallery" href="#" data-picnum="13"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513841.jpg" class="top_images" rel="gallery" title="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513841.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr<a class="openGallery" href="#" data-picnum="14"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512696.jpg" class="top_images" rel="gallery" title="Immunohistochemical localization of transporters in anterior and posterior intestine of tilapia acclimated to different salinities.Representative micrographs of immunolocalization of Nka (green) with Nkcc/Ncc (red) (a–d, f–h) from FW (a, f), SW (b, g) and HSW (c, h) acclimated tilapia. (d) A representative higher magnification micrograph of Nkcc/Ncc staining of the brush border of enterocytes with basolateral Nka staining from the anterior intestine of SW-acclimated fish. (e) Apical Cftr (red) double labeling with Nka (green) in the anterior intestine of a FW-acclimated fish. Panels (a–e) are sections of anterior intestine (AI) while panels (f–h) are sections of posterior intestine (PI). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Scale bar 100 µm (a–c, f–h); 25 µm (d,e). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412512696.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemical localization of transporters in anterior and posterior intestine of tilapia acclimated to different salinities.Representative micrographs of immunolocalization of Nka (green) with Nkcc/Ncc (red) (a–d, f–h) from FW (a, f), SW (b, g) and HSW (c, h) acclimated tilapia. (d) A representative higher magnification micrograph of Nkcc/Ncc staining of the brush border of enterocytes with basolateral Nka staining from the anterior intestine of SW-acclimated fish. (e) Apical Cftr (red) double labeling with Nka (green) in the anterior intestine of a FW-acclimated fish. Panels (a–e) are sections of anterior intestine (AI) while panels (f–h) are sections of posterior intestine (PI). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Scale bar 100 µm (a–c, f–h); 25 µm (d,e). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Immunohistochemical localization of transporters in anterior and posterior intestine of tilapia acclimated to different salinities.Representative micrographs of immunolocalization of Nka (green) with Nkcc/Ncc (red) (a–d, f–h) from FW (a, f), SW (b, g) and HSW (c, h) acclimated tilapia. (d) A representative higher magnification micrograph of Nkcc/Ncc staining of the brush border of enterocytes with basolateral Nka staining from the anterior intestine of SW-acclimated fish. (e) Apical Cftr (red) double labeling with Nka (green) in the anterior intestine of a FW-acclimated fish. Panels (a–e) are sections of anterior intestine (AI) while panels (f–h) are sections of posterior intestine (PI). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Scale bar 100 µm (a–c, f–h); 25 µm (d,e). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemical localization of transporters in anterior and posterior intestine of tilapia acclimated to different salinities.Representative micrographs of immunolocalization of Nka (green) with Nkcc/Ncc (red)<a class="openGallery" href="#" data-picnum="15"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Immunohistochemistry'>IHC</span>, <span title='Immunoprecipitation'>IP</span></td> </tr><tr class="lined"> <td><strong>Clone</strong></td> <td><div>24-1</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Monoclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Mouse</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="Human CFTR C-Terminus MAb (Clone 24-1)" data-catnum="MAB25031" data-catnums="MAB25031,MAB25031-SP" data-disabled=''><noscript><form autocomplete="off" action="/products/cftr-antibody-24-1_mab25031" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size / Catalog#</th><th>Qty</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div data-msg='' 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class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/antibodies/human-cftr-c-terminus-antibody-24-1_mab25031" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>CFTR Antibody (24-1) [Unconjugated] - C-terminus Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>Glutathione S-transferase-coupled CFTR<br />aa 1377-1480<br />Accession # P13569</div></td> </tr><tr class="lined"> <td><strong>Specificity</strong></td> <td><div>Detects <EM>in vitro</EM> synthesized CFTR and endogenous CFTR in Western blots.</div></td> </tr><tr class="lined"> <td><strong>Source</strong></td> <td><div>N/A</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG2a</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Monoclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Mouse</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>CFTR</div></td> </tr><tr class="lined"> <td><strong>Purity Statement</strong></td> <td><div>Protein A or G purified from hybridoma culture supernatant</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Immunohistochemistry 8-25 ug/mL</li><li>Immunoprecipitation 1-2 ug/10^6 cells</li><li>Western Blot 1 ug/mL</li> </ul> </td> </tr><tr class="lined"> <td><strong>Reviewed Applications</strong></td> <td> <table class="wide"> <tr> <td> Read <a href="#ReviewsSection">10 Reviews</a> rated 4.2<div class="dsSprite_stars4"></div> using <br/>MAB25031 in the following applications:<br /><br /> <ul class="information-list"><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Western Blot">Western Blot</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunofluorescence">Immunofluorescence</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunoprecipitation">Immunoprecipitation</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunohistochemistry-Frozen">Immunohistochemistry-Frozen</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Flow Cytometry">Flow Cytometry</a></div> <div class="information-data"></div> </li> </ul> </td> </tr> </table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> MAB25031 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Co-Immunoprecipitation">Co-Immunoprecipitation</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Flow Cytometry">Flow Cytometry</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ICC">ICC</a> </div><div class="information-data">13 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC">IHC</a> </div><div class="information-data">4 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-Fr">IHC-Fr</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-P">IHC-P</a> </div><div class="information-data">5 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunoprecipitation">Immunoprecipitation</a> </div><div class="information-data">10 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Western Blot">Western Blot</a> </div><div class="information-data">26 publications</div></li></ul></td> </tr> </table> </td> </tr> </table> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>No Preservative</div></td> </tr><tr class="lined"> <td><strong>Reconstitution Instructions</strong></td> <td><div>Reconstitute at 0.5 mg/mL in sterile PBS.</div></td> </tr></table></div> <h2 class="greyHead2">Notes</h2><div class="ds_info"> <a id="tab1notes"></a> <div>This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.</div> </div><h2 class="greyHead2">Alternate Names for CFTR Antibody (24-1) [Unconjugated] - C-terminus</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>ABC35</li><li>ABCC7CF</li><li>ATP-binding cassette sub-family C member 7</li><li>ATP-binding cassette transporter sub-family C member 7</li><li>cAMP-dependent chloride channel</li><li>CFTR</li><li>CFTR/MRP</li><li>Channel conductance-controlling ATPase</li><li>cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)</li><li>cystic fibrosis transmembrane conductance regulator</li><li>cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)</li><li>dJ760C5.1</li><li>EC 3.6.3</li><li>MRP7EC 3.6.3.49</li><li>TNR-CFTR</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. 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Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14184-0), licensed under a CC-BY license." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/SPINK1-Antibody-4D4-Immunohistochemistry-H00006690-M01-img0012.jpg" alt="Immunohistochemistry SPINK1 Antibody (4D4)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/spink1-antibody-4d4_h00006690-m01" title="Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - Representative images showing H&amp;E staining (x200 magnification) and immunostaining (x200 magnification) for AR, synaptophysin, and SPINK1 in tumor specimens obtained from NEPC patients', namely WCM12, WCM155 (an organoid), and WCM677. Scale bar represents 100um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14184-0), licensed under a CC-BY license." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/SPINK1-Antibody-4D4-Immunohistochemistry-H00006690-M01-img0013.jpg" alt="Immunohistochemistry SPINK1 Antibody (4D4)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/spink1-antibody-4d4_h00006690-m01#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/spink1-antibody-4d4_h00006690-m01#PublicationSection"> <div class="sprite_pubBook"></div> (32 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-91642"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/abcb6-antibody_nbp1-91642" style="overflow: hidden; width: 280px;" title="ABCB6 Antibody">ABCB6 Antibody</a>    </div><div class="sub-header">NBP1-91642</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/abcb6-antibody_nbp1-91642" title="Immunohistochemistry-Paraffin: ABCB6 Antibody [NBP1-91642] - Staining of human testis shows cytoplasmic positivity." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/ABCB6-Antibody-Immunohistochemistry-Paraffin-NBP1-91642-img0004.jpg" alt="Immunohistochemistry-Paraffin ABCB6 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/abcb6-antibody_nbp1-91642" title="Immunocytochemistry/Immunofluorescence: ABCB6 Antibody [NBP1-91642] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/ABCB6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91642-img0003.jpg" alt="Immunocytochemistry/ Immunofluorescence ABCB6 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/abcb6-antibody_nbp1-91642#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB200-541"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/complement-c4b-d-antibody-16d2_nb200-541" style="overflow: hidden; width: 280px;" title="Complement C4b/d Antibody (16D2)">Complement C4b/d<br> Antibody (16D2)</a>    </div><div class="sub-header">NB200-541</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/complement-c4b-d-antibody-16d2_nb200-541" title="Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/Complement-C4b-d-Antibody-16D2-Western-Blot-NB200-541-img0001.jpg" alt="Western Blot Complement C4b/d Antibody (16D2)" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/complement-c4b-d-antibody-16d2_nb200-541#PublicationSection"> <div class="sprite_pubBook"></div> (12 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> IA, ICC/IF, IHC, IHC-Fr, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF4928"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ermap-antibody_af4928" style="overflow: hidden; width: 280px;" title="ERMAP Antibody [Unconjugated]">ERMAP Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF4928</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/ermap-antibody_af4928" title="K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human ERMAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4928, filled histogram) or control antibody (Catalog&amp;nbsp;# AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# F0108)." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERMAP_AF4928_Flow_Cytometry_11957.jpg" alt="Flow Cytometry ERMAP Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF3848"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/trypsin-1-prss1-antibody_af3848" style="overflow: hidden; width: 280px;" title="Trypsin 1/PRSS1 Antibody [Unconjugated]">Trypsin 1/PRSS1<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF3848</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/trypsin-1-prss1-antibody_af3848" title="Trypsin 1/PRSS1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Trypsin 1/PRSS1 Monoclonal Antibody (Catalog # MAB3848) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images2/Trypsin_1_AF3848_Immunohistochemistry_17574.jpg" alt="Immunohistochemistry Trypsin 1/PRSS1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/trypsin-1-prss1-antibody_af3848#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/trypsin-1-prss1-antibody_af3848#PublicationSection"> <div class="sprite_pubBook"></div> (7 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP3-23927"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pet1-antibody-fev-7311_nbp3-23927" style="overflow: hidden; width: 280px;" title="Pet1 Antibody (FEV/7311)">Pet1 Antibody<br> (FEV/7311)</a>    </div><div class="sub-header">NBP3-23927</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/pet1-antibody-fev-7311_nbp3-23927" title="Formalin-fixed, paraffin-embedded human pancreas stained with Pet1 antibody (FEV/7311). Inset: PBS instead of primary antibody; secondary only negative control." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-23927_mouse-pet1-mab-fev-7311-1312202311505362.jpg" alt="Immunohistochemistry-Paraffin Pet1 Antibody (FEV/7311)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pet1-antibody-fev-7311_nbp3-23927" title="Formalin-fixed, paraffin-embedded human kidney stained with Pet1 antibody (FEV/7311). Inset: PBS instead of primary antibody; secondary only negative control." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-23927_mouse-pet1-mab-fev-7311-1312202311502960.jpg" alt="Immunohistochemistry-Paraffin Pet1 Antibody (FEV/7311)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pet1-antibody-fev-7311_nbp3-23927" title="Formalin-fixed, paraffin-embedded human thyroid stained with Pet1 antibody (FEV/7311). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-23927_mouse-pet1-mab-fev-7311-1312202311502944.jpg" alt="Immunohistochemistry-Paraffin Pet1 Antibody (FEV/7311)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC-P</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB400-156"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mrp1-antibody-iu2h10_nb400-156" style="overflow: hidden; width: 280px;" title="MRP1 Antibody (IU2H10) - BSA Free">MRP1 Antibody<br> (IU2H10) - BSA Free</a>    </div><div class="sub-header">NB400-156</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/mrp1-antibody-iu2h10_nb400-156" title="Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/MRP1-Antibody-IU2H10-Dual-RNAscope-ISH-IHC-NB400-156-img0008.jpg" alt="Dual RNAscope ISH-IHC MRP1 Antibody (IU2H10) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mrp1-antibody-iu2h10_nb400-156" title="Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)" class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/MRP1-Antibody-IU2H10-Western-Blot-NB400-156-img0006.jpg" alt="Western Blot MRP1 Antibody (IU2H10) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mrp1-antibody-iu2h10_nb400-156" title="Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/MRP1-Antibody-IU2H10-Immunocytochemistry-Immunofluorescence-NB400-156-img0007.jpg" alt="Immunocytochemistry/ Immunofluorescence MRP1 Antibody (IU2H10) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mrp1-antibody-iu2h10_nb400-156#PublicationSection"> <div class="sprite_pubBook"></div> (17 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IP, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="M6000B"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" style="overflow: hidden; width: 280px;" title="IL-6 [HRP]">IL-6 [HRP]</a>    </div><div class="sub-header">M6000B</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="" class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="" class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="Increased production of proinflammatory cytokines and VEGF in the plasma and chondrocytes in high fat diet (HFD)-feeding mice.(a~d) levels of plasma IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the C57BL/6 male mice fed with a normal diet (ND) or HFD for 8 weeks. (n = 5, **P &lt; 0.01). (e~h) Relative mRNA expression of IL-6 (e), TNF-alpha (f), IL-1 beta (g) and VEGF (h) in the chondrocytes isolated from the 8-week ND or HFD-feeding mice. (n = 5, **P &lt; 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26271607), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b#PublicationSection"> <div class="sprite_pubBook"></div> (656 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-84999"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/dnase1-antibody_nbp1-84999" style="overflow: hidden; width: 280px;" title="DNASE1 Antibody">DNASE1 Antibody</a>    </div><div class="sub-header">NBP1-84999</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/dnase1-antibody_nbp1-84999" title="Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Analysis in human duodenum and liver tissues using Anti-DNASE1 antibody. Corresponding DNASE1 RNA-seq data are presented for the same tissues." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0011.jpg" alt="Immunohistochemistry-Paraffin DNASE1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/dnase1-antibody_nbp1-84999" title="Western Blot: DNASE1 Antibody [NBP1-84999] - Analysis in control (vector only transfected HEK293T lysate) and DNASE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/DNASE1-Antibody-Western-Blot-NBP1-84999-img0006.jpg" alt="Western Blot DNASE1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/dnase1-antibody_nbp1-84999" title="Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Staining of human liver shows low expression as expected." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0013.jpg" alt="Immunohistochemistry-Paraffin DNASE1 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/dnase1-antibody_nbp1-84999#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DY4517-05"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mouse-neutrophil-elastase-ela2-duoset-elisa-5-plate_dy4517-05" style="overflow: hidden; width: 280px;" title="Neutrophil Elastase/ELA2 [Biotin]">Neutrophil<br> Elastase/ELA2 [Biotin</a>    </div><div class="sub-header">DY4517-05</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/mouse-neutrophil-elastase-ela2-duoset-elisa-5-plate_dy4517-05" title="" class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_Neutrophil_Elastase_DY451705_ELISA_2461.jpg" alt="N/A Neutrophil Elastase/ELA2 [Biotin]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mouse-neutrophil-elastase-ela2-duoset-elisa-5-plate_dy4517-05#PublicationSection"> <div class="sprite_pubBook"></div> (15 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-34294"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/thyroglobulin-antibody-2h11-6e1_nbp2-34294" style="overflow: hidden; width: 280px;" title="Thyroglobulin Antibody (2H11 + 6E1)">Thyroglobulin<br> Antibody (2H11 + 6E1)</a>    </div><div class="sub-header">NBP2-34294</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/thyroglobulin-antibody-2h11-6e1_nbp2-34294" title="Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1)." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Thyroglobulin-Antibody-2H11-+-6E1-Immunohistochemistry-Paraffin-NBP2-34294-img0003.jpg" alt="Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/thyroglobulin-antibody-2h11-6e1_nbp2-34294" title="Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Thyroglobulin-Antibody-2H11-+-6E1-Immunohistochemistry-NBP2-34294-img0004.jpg" alt="Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/thyroglobulin-antibody-2h11-6e1_nbp2-34294" title="Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. IHC-P image submitted by a verified customer review." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Thyroglobulin-Antibody-2H11-+-6E1-Immunohistochemistry-Paraffin-NBP2-34294-img0005.jpg" alt="Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/thyroglobulin-antibody-2h11-6e1_nbp2-34294#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/thyroglobulin-antibody-2h11-6e1_nbp2-34294#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, IHC, IHC-P</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB1417"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/insulin-antibody-182410_mab1417" style="overflow: hidden; width: 280px;" title="Insulin Antibody (182410) [Unconjugated]">Insulin Antibody<br> (182410) [Unconjugate ...</a>    </div><div class="sub-header">MAB1417</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/insulin-antibody-182410_mab1417" title="Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg" alt="Immunocytochemistry Insulin Antibody (182410) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/insulin-antibody-182410_mab1417" title="Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5&amp;#160;&amp;micro;g/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte&amp;trade; HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg" alt="Immunohistochemistry Insulin Antibody (182410) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/insulin-antibody-182410_mab1417#PublicationSection"> <div class="sprite_pubBook"></div> (24 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, ICC, IHC, ICFlow</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-45731"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/beta-galactosidase-1-glb1-antibody-oti1c9_nbp2-45731" style="overflow: hidden; width: 280px;" title="beta-Galactosidase-1/GLB1 Antibody (OTI1C9)"><br>beta-Galactosidase-1/G ...</a>    </div><div class="sub-header">NBP2-45731</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/beta-galactosidase-1-glb1-antibody-oti1c9_nbp2-45731" title="Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/beta-Galactosidase-1-GLB1-Antibody-1C9-Western-Blot-NBP2-45731-img0016.jpg" alt="Western Blot beta-Galactosidase-1/GLB1 Antibody (OTI1C9)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/beta-galactosidase-1-glb1-antibody-oti1c9_nbp2-45731" title="Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/beta-Galactosidase-1-GLB1-Antibody-1C9-Immunohistochemistry-NBP2-45731-img0001.jpg" alt="Immunohistochemistry beta-Galactosidase-1/GLB1 Antibody (OTI1C9)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/beta-galactosidase-1-glb1-antibody-oti1c9_nbp2-45731" title="Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/beta-Galactosidase-1-GLB1-Antibody-1C9-Immunohistochemistry-NBP2-45731-img0002.jpg" alt="Immunohistochemistry beta-Galactosidase-1/GLB1 Antibody (OTI1C9)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/beta-galactosidase-1-glb1-antibody-oti1c9_nbp2-45731#PublicationSection"> <div class="sprite_pubBook"></div> (6 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Ca, Hu, Pm</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB25031"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cftr-antibody-24-1_mab25031" style="overflow: hidden; width: 280px;" title="CFTR Antibody (24-1) [Unconjugated] - C-terminus">CFTR Antibody<br> (24-1) [Unconjugated] ...</a>    </div><div class="sub-header">MAB25031</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="cust_viewed_15"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="CFTR Antibody (24-1) [Unconjugated] - C-terminus" title="CFTR Antibody (24-1) [Unconjugated] - C-terminus" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> WB, IHC, IP</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for CFTR Antibody (MAB25031)(76)</h2><div class="ds_info"> <div>We have publications tested in 11 confirmed species: Human, Mouse, Rat, Amphibian, Fish, Fish - Goldline seabream, Fish - Plotosus lineatus (Striped Eel Catfish), Ovine, Primate - Cercopithecus aethiops (African Green Monkey), Primate - Chlorocebus pygerythrus (Vervet Monkey), Xenopus.<br /><br />We have publications tested in 8 applications: Co-Immunoprecipitation, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Co-Immunoprecipitation"> <div style="display:inline;">Co-Immunoprecipitation</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Flow Cytometry"> <div style="display:inline;">Flow Cytometry</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ICC"> <div style="display:inline;">ICC</div> (13)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC"> <div style="display:inline;">IHC</div> (4)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-Fr"> <div style="display:inline;">IHC-Fr</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-P"> <div style="display:inline;">IHC-P</div> (5)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Immunoprecipitation"> <div style="display:inline;">Immunoprecipitation</div> (10)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Western Blot"> <div style="display:inline;">Western Blot</div> (26)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (34)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (2)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Amphibian"> <div style="display:inline;">Amphibian</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Fish"> <div style="display:inline;">Fish</div> (3)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Fish - Goldline seabream"> <div style="display:inline;">Fish - Goldline seabream</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Fish - Plotosus lineatus (Striped Eel Catfish)"> <div style="display:inline;">Fish - Plotosus lineatus (Striped Eel Catfish)</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Ovine"> <div style="display:inline;">Ovine</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Primate - Cercopithecus aethiops (African Green Monkey)"> <div style="display:inline;">Primate - Cercopithecus aethiops (African Green Monkey)</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Primate - Chlorocebus pygerythrus (Vervet Monkey)"> <div style="display:inline;">Primate - Chlorocebus pygerythrus (Vervet Monkey)</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Xenopus"> <div style="display:inline;">Xenopus</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">10</span> of 76. <a class="pubs_showall">Show All 76 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a><table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using MAB25031</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="Western Blot" data-species="Primate - Cercopithecus aethiops (African Green Monkey)"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/39043712" target="_blank" rel="nofollow"> Caohuy, H;Eidelman, O;Chen, T;Mungunsukh, O;Yang, Q;Walton, NI;Pollard, BS;Khanal, S;Hentschel, S;Florez, C;Herbert, AS;Pollard, HB; Inflammation in the COVID-19 airway is due to inhibition of CFTR signaling by the SARS-CoV-2 spike protein Scientific reports 2024-07-23 [PMID: 39043712] (Western Blot, Primate - Cercopithecus aethiops (African Green Monkey)) </a></td> <td valign="top">Western Blot</td> <td valign="top">Primate - Cercopithecus aethiops (African Green Monkey)</td> </tr><tr class="lined revfil firstten" data-application="ICC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/38019847" target="_blank" rel="nofollow"> Li, C;Liu, Z;Anderson, J;Liu, Z;Tang, L;Li, Y;Peng, N;Chen, J;Liu, X;Fu, L;Townes, TM;Rowe, SM;Bedwell, DM;Guimbellot, J;Zhao, R; Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells PloS one 2023-11-29 [PMID: 38019847] (ICC, Human) </a></td> <td valign="top">ICC</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35665694" target="_blank" rel="nofollow"> Qin-Tong Hou, Xiao-Liang Xu, Jian-Wen Wang, Xiao-Liang Dai, Li Wang, Cong-Ling Jiang et al. Loss-of-function CFTR p.G970D missense mutation might cause congenital bilateral absence of the vas deferens and be associated with impaired spermatogenesis Asian Journal of Andrology 2023-01-01 [PMID: 35665694] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35210618" target="_blank" rel="nofollow"> Nathaniel J. Henning, Lydia Boike, Jessica N. Spradlin, Carl C. Ward, Gang Liu, Erika Zhang et al. 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J Immunol, 2014-04-14;192(10):4804-12. 2014-04-14 [PMID: 24733844] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Fish"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24498146" target="_blank" rel="nofollow"> Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water. PLoS ONE, 2014-01-31;9(1):e87591. 2014-01-31 [PMID: 24498146] (IHC-P, Fish) </a></td> <td valign="top">IHC-P</td> <td valign="top">Fish</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Primate - Chlorocebus pygerythrus (Vervet Monkey)"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24416359" target="_blank" rel="nofollow"> Cebotaru L, Rapino D, Cebotaru V, Guggino W Correcting the cystic fibrosis disease mutant, A455E CFTR. PLoS ONE, 2014-01-08;9(1):e85183. 2014-01-08 [PMID: 24416359] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey)) </a></td> <td valign="top">Western Blot</td> <td valign="top">Primate - Chlorocebus pygerythrus (Vervet Monkey)</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24204804" target="_blank" rel="nofollow"> Barbara Dhooghe, Sabrina Noël, Caroline Bouzin, Gaëtane Behets-Wydemans, Teresinha Leal Correction of Chloride Transport and Mislocalization of CFTR Protein by Vardenafil in the Gastrointestinal Tract of Cystic Fibrosis Mice PLoS ONE 2013-10-24 [PMID: 24204804] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24019521" target="_blank" rel="nofollow"> El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR). J Biol Chem, 2013-09-09;288(43):31177-91. 2013-09-09 [PMID: 24019521] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23560079" target="_blank" rel="nofollow"> Viart V, Varilh J, Lopez E, Rene C, Claustres M, Taulan-Cadars M Phosphorylated C/EBPbeta influences a complex network involving YY1 and USF2 in lung epithelial cells. PLoS ONE, 2013-04-01;8(4):e60211. 2013-04-01 [PMID: 23560079] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23436935" target="_blank" rel="nofollow"> Oglesby I, Chotirmall S, McElvaney N, Greene C Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in DeltaF508 cystic fibrosis airway epithelium. J Immunol, 2013-02-22;190(7):3354-62. 2013-02-22 [PMID: 23436935] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23045527" target="_blank" rel="nofollow"> Duan , Yuanyuan, Sun , Ying, Zhang , Fan, Zhang , Wei Kevi, Wang , Dong, Wang , Yan, Cao , Xu, Hu , Wenbao, Xie , Changyan, Cuppoletti , John, Magin , Thomas M, Wang , Haixia, Wu , Zhenguo, Li , Ning, Huang , Pingbo Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch. J Biol Chem, 2012-10-08;287(48):40547-59. 2012-10-08 [PMID: 23045527] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Flow Cytometry, ICC, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23029546" target="_blank" rel="nofollow"> Murphy , Sean V, Lim , Rebecca, Heraud , Philip, Cholewa , Marian, Le Gros , Mark, de Jonge , Martin D, Howard , Daryl L, Paterson , David, McDonald , Courtney, Atala , Anthony, Jenkin , Graham, Wallace , Euan M Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator. PLoS ONE, 2012-09-28;7(9):e46533. 2012-09-28 [PMID: 23029546] (Flow Cytometry, ICC, Western Blot, Human) </a></td> <td valign="top">Flow Cytometry, ICC, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22869372" target="_blank" rel="nofollow"> King , J Darwin, Lee , Jeffrey, Riemen , Claudia, Neumann , Dietbert, Xiong , Sheng, Foskett , J Kevin, Mehta , Anil, Muimo , Richmond, Hallows , Kenneth Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase. J Biol Chem, 2012-08-06;287(40):33389-400. 2012-08-06 [PMID: 22869372] (Immunoprecipitation, Western Blot, Human) </a></td> <td valign="top">Immunoprecipitation, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22768251" target="_blank" rel="nofollow"> Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouve P, Ferec C Proteomic Identification of Calumenin as a G551D - CFTR Associated Protein. PLoS ONE, 2012-06-29;7(6):e40173. 2012-06-29 [PMID: 22768251] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22315512" target="_blank" rel="nofollow"> Valentina Paracchini, Annalucia Carbone, Federico Colombo, Stefano Castellani, Silvia Mazzucchelli, Sante Di Di Gioia et al. Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells Journal of Biomedicine and Biotechnology 2012-01-22 [PMID: 22315512] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21811577" target="_blank" rel="nofollow"> Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis. PLoS ONE, 2011-07-21;6(7):e22212. 2011-07-21 [PMID: 21811577] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21561312" target="_blank" rel="nofollow"> Donatella Piro, Claudia Piccoli, Lorenzo Guerra, Francesca Sassone, Annamaria D'Aprile, Maria Favia et al. Hematopoietic Stem/Progenitor Cells Express Functional Mitochondrial Energy-Dependent Cystic Fibrosis Transmembrane Conductance Regulator Stem Cells and Development 2012-03-01 [PMID: 21561312] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21483833" target="_blank" rel="nofollow"> Drevillon L, Tanguy G, Hinzpeter A et al. COMMD1-Mediated Ubiquitination Regulates CFTR Trafficking. PLoS One. 3/31/2011 [PMID: 21483833] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/20089165" target="_blank" rel="nofollow"> Jean-Marie Zahm, Franck Delavoie, Férial Toumi, Béatrice Nawrocki-Raby, Claire Kileztky, Jean Michel et al. Long acting beta2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant Respiratory Research 2010-12-01 [PMID: 20089165] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/20004757" target="_blank" rel="nofollow"> Antonius Bronckers, Lida Kalogeraki, Huub J.N. Jorna, Martina Wilke, Theodore J. Bervoets, Donacian M. Lyaruu et al. The cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in maturation stage ameloblasts, odontoblasts and bone cells Bone 2010-04-01 [PMID: 20004757] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19840964" target="_blank" rel="nofollow"> D. J. McKeon, K. A. Cadwallader, S. Idris, A. S. Cowburn, M. C. Pasteur, H. Barker et al. Cystic fibrosis neutrophils have normal intrinsic reactive oxygen species generation European Respiratory Journal 2010-06-01 [PMID: 19840964] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/19234136" target="_blank" rel="nofollow"> Fouassier L, Rosenberg P, Mergey M, Saubamea B, Claperon A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation. Am. J. Pathol., 2009-03-01;174(3):869-80. 2009-03-01 [PMID: 19234136] (IHC, Human) </a></td> <td valign="top">IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/18508776" target="_blank" rel="nofollow"> Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing. J. Biol. Chem., 2008-05-28;283(32):21926-33. 2008-05-28 [PMID: 18508776] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC, IHC, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17600296" target="_blank" rel="nofollow"> Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone. Thorax, 2007-07-01;62(7):650-1. 2007-07-01 [PMID: 17600296] (ICC, IHC, Western Blot, Human) </a></td> <td valign="top">ICC, IHC, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC, Immunoprecipitation" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17596272" target="_blank" rel="nofollow"> Robert R, Savineau JP, Norez C, Becq F, Guibert C Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries. Eur. Respir. J., 2007-06-27;30(5):857-64. 2007-06-27 [PMID: 17596272] (ICC, Immunoprecipitation, Rat) </a></td> <td valign="top">ICC, Immunoprecipitation</td> <td valign="top">Rat</td> </tr><tr class="lined revfil hidden" data-application="IHC-P, Western Blot" data-species="Fish"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17210762" target="_blank" rel="nofollow"> Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. J. Endocrinol., 2007-01-01;192(1):249-60. 2007-01-01 [PMID: 17210762] (IHC-P, Western Blot, Fish) </a></td> <td valign="top">IHC-P, Western Blot</td> <td valign="top">Fish</td> </tr><tr class="lined revfil hidden" data-application="ICC, Western Blot" data-species="Fish - Goldline seabream"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17161378" target="_blank" rel="nofollow"> Tang JY, Au DW The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo. Biochem. Biophys. Res. Commun., 2006-12-04;353(1):98-103. 2006-12-04 [PMID: 17161378] (ICC, Western Blot, Fish - Goldline seabream) </a></td> <td valign="top">ICC, Western Blot</td> <td valign="top">Fish - Goldline seabream</td> </tr><tr class="lined revfil hidden" data-application="ICC, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/16922501" target="_blank" rel="nofollow"> Painter RG, Valentine VG, Lanson NA, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis. Biochemistry, 2006-08-29;45(34):10260-9. 2006-08-29 [PMID: 16922501] (ICC, Western Blot, Human) </a></td> <td valign="top">ICC, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ICC, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/16530168" target="_blank" rel="nofollow"> Nanua S, Sajjan U, Keshavjee S, Hershenson MB Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells. Biochem. Biophys. Res. Commun., 2006-03-03;343(1):135-43. 2006-03-03 [PMID: 16530168] (ICC, Western Blot, Human) </a></td> <td valign="top">ICC, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC-Fr" data-species="Ovine"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/16498081" target="_blank" rel="nofollow"> Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):72-83. 2006-02-23 [PMID: 16498081] (IHC-Fr, Ovine) </a></td> <td valign="top">IHC-Fr</td> <td valign="top">Ovine</td> </tr><tr class="lined revfil hidden" data-application="ICC, Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/15776432" target="_blank" rel="nofollow"> Clain J, Lehmann-Che J, Dugueperoux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype. Hum. Mutat., 2005-04-01;25(4):360-71. 2005-04-01 [PMID: 15776432] (ICC, Immunoprecipitation, Human) </a></td> <td valign="top">ICC, Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/15039462" target="_blank" rel="nofollow"> Bilan F, Thoreau V, Nacfer M, Derand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity. J. Cell. Sci., 2004-03-23;117(0):1923-35. 2004-03-23 [PMID: 15039462] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC-P" data-species="Xenopus"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/12421535" target="_blank" rel="nofollow"> Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH Mitochondria-rich cells as experimental model in studies of epithelial chloride channels. Biochim. Biophys. Acta, 2002-11-13;1566(1):28-43. 2002-11-13 [PMID: 12421535] (IHC-P, Xenopus) </a></td> <td valign="top">IHC-P</td> <td valign="top">Xenopus</td> </tr><tr class="lined revfil hidden" data-application="ICC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/12397014" target="_blank" rel="nofollow"> Pan J, Bear C, Farragher S, Cutz E, Yeger H Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models. Am. J. Respir. Cell Mol. Biol., 2002-11-01;27(5):553-60. 2002-11-01 [PMID: 12397014] (ICC, Human) </a></td> <td valign="top">ICC</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Amphibian"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/11937500" target="_blank" rel="nofollow"> Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model. J. Biol. Chem., 2002-04-05;277(24):21480-8. 2002-04-05 [PMID: 11937500] (Western Blot, Amphibian) </a></td> <td valign="top">Western Blot</td> <td valign="top">Amphibian</td> </tr><tr><td colspan="3" align="center"> <a class="pubs_showall">Show All 76 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a> </td></tr></tbody> </table> </div> </div><a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>CFTR Antibody (MAB25031)</span> (10) <span style="display:none;">4.2</span><span style="display:none;">10</span></h2> <div class="ds_info"> <div class="bottom15"> <strong>Average Rating: 4.2</strong> <div class="dsSprite_stars4"></div> (Based on 10 reviews) </div> <div><div class="ds_info">We have 10 reviews tested in 3 species: Human, Mouse, Other.</div> <div class="align-right"> <button class="greybutton btn_width" id="novusreview" name="novusreview" onclick="window.location='/reviews/MAB25031'"; >Submit a Review</button><br /> </div> </div> <div class="blueHead"><strong>Reviews using MAB25031:</strong></div> <div class="filterParent"><div class="reviews_filterList"> <div class="blueHead">Filter by Applications</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (3)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="IF"> <div style="display:inline;">IF</div> (4)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="IP"> <div style="display:inline;">IP</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="IHC-Fr"> <div style="display:inline;">IHC-Fr</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="Flow"> <div style="display:inline;">Flow</div> (1)</div><div class="filter_clear" data-type="review" data-filter="application">All Applications</div></div> <div class="reviews_filterList"> <div class="blueHead">Filter by Species</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (5)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (4)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Other"> <div style="display:inline;">Other</div> (1)</div><div class="filter_clear" data-type="review" data-filter="species">All Species</div></div></div><div id="reviews_list"> <table class="wide lightPad tablesorter" id="reviews_list_table"> <thead> <tr> <th>Images</th> <th>Ratings</th> <th>Applications</th> <th>Species</th> <th>Date</th> <th>Details</th> </tr> </thead> <tbody><tr class="top-lined revfil" data-rating="Excellent" data-application="WB" data-species="Mouse"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 23060444</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Excellent" data-application="IF" data-species="Other"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunofluorescence">IF</td> <td>Other</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunofluorescence</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 20107177</td></tr><tr><td class="text_bold">Species</td><td>Other</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Human"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Human</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 21811577</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Human"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Human</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 22904170</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IP" data-species="Human"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunoprecipitation">IP</td> <td>Human</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunoprecipitation</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 21383017</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IHC-Fr" data-species="Mouse"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunohistochemistry-Frozen">IHC-Fr</td> <td>Mouse</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunohistochemistry-Frozen</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 23045527</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IF" data-species="Mouse"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunofluorescence">IF</td> <td>Mouse</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunofluorescence</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 24204804</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IF" data-species="Mouse"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunofluorescence">IF</td> <td>Mouse</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunofluorescence</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 22989883</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="IF" data-species="Human"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunofluorescence">IF</td> <td>Human</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunofluorescence</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 20351101</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="Flow" data-species="Human"> <td> <meta content="02/19/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Flow Cytometry">Flow</td> <td>Human</td> <td>02/19/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Flow Cytometry</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 21561312</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr></table> </div> </div> </td> </tr></tbody> </table> </div><br /> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/immunoprecipitation/protocol.html">Immunoprecipitation</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for CFTR Antibody (MAB25031) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Mouse&facet_conjugate=HRP">Anti-Mouse HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Mouse&facet_conjugate=FITC">Anti-Mouse FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Mouse&facet_conjugate=Biotin">Anti-Mouse Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Mouse%22">View all of our anti-Mouse Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Mouse%22">Mouse Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional CFTR Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array MAB25031</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/cftr">CFTR Antibodies</a></li><li> <a href="/elisa-kits/cftr">CFTR ELISA Kit</a></li><li> <a href="/proteins/cftr">CFTR Proteins</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on CFTR. </h2> <a id="blogsect"></a> <div class="ds_info"><a href="/antibody-news/tag/cftr">Check out the latest blog posts on CFTR</a>.</div> <div class="ds_info"> <table id="blogs_list_table"> <tbody> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/the-abca1-antibody-and-abc-transporter-research-2">The ABCA1 Antibody and ABC Transporter Research</a></strong><br/> <span>The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  <a href="/antibody-news/antibodies/the-abca1-antibody-and-abc-transporter-research-2">Read full blog post.</a></span><br /><br /> </td> </tr></tbody> </table> </div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="https://www.bio-techne.com/resources/blogs/citation-widget-find-products-used-in-literature" target="_blank"><img loading="lazy" src="https://images.novusbio.com/design/citation-app-banner-349x119-v3.jpg" alt="Read our latest blog and use the new citation tool on bio-techne.com" title="new citation tool" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="MAB003"> <div class="img_container"> <a href="/products/igg2a-isotype-control-20102_mab003" title="Human peripheral blood granulocytes were stained with MouseAnti-HumanCXCR1/IL-8&nbsp;RA Monoclonal Antibody (Catalog&nbsp;# MAB330,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog&nbsp;# MAB003,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog&nbsp;# F0102B)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG2A_MAB003_Control_19426.jpg" title="Human peripheral blood granulocytes were stained with MouseAnti-HumanCXCR1/IL-8&nbsp;RA Monoclonal Antibody (Catalog&nbsp;# MAB330,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog&nbsp;# MAB003,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog&nbsp;# F0102B)." alt="Human peripheral blood granulocytes were stained with MouseAnti-HumanCXCR1/IL-8&nbsp;RA Monoclonal Antibody (Catalog&nbsp;# MAB330,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog&nbsp;# MAB003,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog&nbsp;# F0102B)." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg2a-isotype-control-20102_mab003">Mouse IgG2a Isotype Control (20102) [Unconjugated]</a> <br />MAB003</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg2a-isotype-control-20102_mab003';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="210-TA-005"> <div class="img_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." alt="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/recombinant-human-tnf-alpha-protein_210-ta">TNF-alpha [Unconjugated]</a> <br />210-TA-005</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/recombinant-human-tnf-alpha-protein_210-ta';" 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id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="concentration" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button"/> </div> </div> </div> </div> </div> <h2 class="greyHead2">Recent Reviews</h2><div class="review_summary"> <div class="review_summary_left"> <div class="review_large_number">4.2</div> <div class="dsSprite_stars4"></div> </div> <div class="review_summary_right"> <table> <tbody><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">5</div></td><td style="position:relative;width:155px;"><div class="review_bar star5" style="width:25%;"></div><div class="review_bar_value">2</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">4</div></td><td style="position:relative;width:155px;"><div class="review_bar star4" style="width:100%;"></div><div class="review_bar_value">8</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">3</div></td><td style="position:relative;width:155px;"><div class="review_bar star3" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div 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