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<!doctype html> <html lang="hi"> <head> <title>Understanding Sample Preparation Workflow: Semi-Automated Pr | 1099778</title> <meta name="keywords" content="Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner, Anubhav Tripathi*, "/> <meta name="description" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the pro..1099778"/> <meta name="citation_publisher" content="लॉन्गडम पब्लिशिंग एसएल" /> <meta name="citation_journal_title" content="एंड्रोलॉजी-ओपन एक्सेस"> <meta name="citation_title" content="Understanding Sample Preparation Workflow: Semi-Automated Preparation of Serum Testosterone for Detection Using LC-MS/MS"> <meta name="citation_author" content="Ramisa Fariha" /> <meta name="citation_author" content="Collin Hill" /> <meta name="citation_author" content="Mohannad Jabrah" /> <meta name="citation_author" content="Adam Spooner" /> <meta name="citation_author" content="Anubhav Tripathi" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="13"> <meta name="citation_issue" content="5"> <meta name="citation_abstract" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS® G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use."> <meta name="citation_abstract_html_url" content="https://hindi.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html"> <meta name="format-detection" content="telephone=no"> <meta http-equiv="Content-Language" content="hi"> <meta name="google-site-verification" content="NomPTP94YozsgvD3NEFpNqUfY88e0TU0L64zNzZTpd0" /> <meta itemprop="name" content="longdom" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="ROBOTS" content="INDEX,FOLLOW"> <meta name="googlebot" content="INDEX,FOLLOW"> <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> <link rel="canonical" 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metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS<sup>® </sup>G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use.</p> <div class="alert alert-info text-left"><b>अस्वीकरण:</b> इस सार का अनुवाद कृत्रिम बुद्धिमत्ता उपकरणों का उपयोग करके किया गया था और अभी तक इसकी समीक्षा या सत्यापन नहीं किया गया है।</div> <div class="nav social-icons"> <a class="nav-link w-auto">इस लेख का हिस्सा</a> <a title="यहाँ क्लिक करें" target="_blank" href="https://www.facebook.com/sharer.php?u=https://hindi.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="यहाँ क्लिक करें" target="_blank" href="https://twitter.com/share?url=https://hindi.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="यहाँ क्लिक करें" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://hindi.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="यहाँ क्लिक करें" target="_blank" href="https://plus.google.com/share?url=https://hindi.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">सामग्री लिंक</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://hindi.longdom.org/privacy-policy.html" title="यहाँ क्लिक करें">गोपनीयता नीति</a></li> <li><a class="" href="https://hindi.longdom.org/terms-conditions.html" title="यहाँ क्लिक करें">नियम एवं शर्तें</a></li> <li><a class="" href="https://hindi.longdom.org/authors-reviewers-editors.html" title="यहाँ क्लिक करें">लेखक, समीक्षक और संपादक</a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">लॉन्गडोम से संपर्क करें</h4> <p><strong>लॉन्गडम ग्रुप एसए</strong><br /> एवेन्यू रोजर वांडेन्ड्रिस्शे,<br /> 18, 1150 ब्रुसेल्स, बेल्जियम<br /> फ़ोन: +442038085340<br/> <strong>ईमेल:</strong> <a class='white' href='mailto:info@longdom.org' title='यहां क्लिक करें'>info@longdom.org</a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">जोड़ना</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="यहाँ क्लिक करें" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="यहाँ क्लिक करें" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="यहाँ क्लिक करें" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="यहाँ क्लिक करें" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>कॉपीराइट © 2024 <a href="https://hindi.longdom.org/" title="यहाँ क्लिक करें" class="white">लॉन्गडम पब्लिशिंग एसएल</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? 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