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GENEWIZ from Azenta | Purified Templates
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href='https://www.azenta.com/' style='color:#826ec3;font-size:17px'>Azenta Life Sciences – Services</a></li><br><li><a class='GColor' href='/en/Public/Services/Next-Generation-Sequencing' style='color:#826ec3;font-size:17px'>Next Generation Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/RNA-Seq' style='color:#585F69;font-size:14px'>RNA-Seq</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Proteomics' style='color:#585F69;font-size:14px'>Proteomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Gene-Expression-Screening' style='color:#585F69;font-size:14px'>High-Throughput Gene Expression Screening <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Single-Cell-Sequencing' style='color:#585F69;font-size:14px'>Single-Cell Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' style='color:#585F69;font-size:14px'>Exome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' style='color:#585F69;font-size:14px'>Amplicon Sequencing Services</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' 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padding: 0; } .walking-new-container { background: #f0f0f0; padding: 30px 0; } .walking-new-container h1 { margin: 0; color: #000000; } .walking-new-content p { color: #000000; } .walking-new-content { width: 1170px; margin: 0 auto; } .walking-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #walking-content-box-left { width: 60%; text-align: left; padding: 30px 0; } #walking-content-box-right { width: 39%; text-align: right; } .walking-content-box img { width: 400px; } .walking-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .walking-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .walking-new-container { text-align: center; margin-top: 0; } .walking-new-content { width: 90%; margin: 0 auto; } .walking-new-content { display: inline-block; } #walking-content-box-right, #walking-content-box-left { width: 100%; text-align: center; } #walking-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .walking-new-content { width: 90%; margin: 0 auto; } .walking-content-box img { width: 350px; } } </style> <div class="walking-new-container"> <div class="walking-new-content"> <div class="walking-content-box" id="walking-content-box-left"> <h1>PURIFIED TEMPLATES</h1> <div> </div> <p>GENEWIZ Multiomics and Synthesis Solutions from Azenta Life Sciences provides reliable, award-winning Sanger DNA Sequencing for purified templates through our Pre-Mixed, Pre-Defined, Custom and Difficult Template sequencing service options. Sanger sequencing from purified templates is our most popular template type. Our high-quality results, industry-leading customer service and fast turnaround times at competitive prices make us the partner of choice for academic, pharmaceutical, GLP, biotechnology, and government institutions. </p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/RegisterAccount/Login" target="_blank"><button>Place Order</button></a> </div> <div class="walking-content-box" id="walking-content-box-right"><a href="https://hubs.ly/Q02FKw0T0" target="_blank"> <img alt="" src="/-/media/11038-D_PEZ-DirectColonySeq_WebBanRight.ashx?la=en&hash=9E030C709843447C76717D11FCC9D4A59384D0CC" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11036-D_Plasmid-ONT_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner1 { width: 100%; height: auto; } .covid-content1 { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <br /> <h3>Ensure Complete Plasmid Validation on Your First Attempt</h3> <h4>with Oxford Nanopore Sequencing</h4> <a href="https://hubs.ly/Q02BX1V70" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container margin-bottom-30"> <h2 style="border-bottom: 1px solid #f1f0f0;">SANGER SEQUENCING OF PURIFIED TEMPLATES</h2> <p>Azenta offers three service levels for Sanger sequencing of purified plasmid and PCR templates: Pre-mixed, Pre-defined, and Custom. <br /> To determine the service that is best for your samples, please see the descriptions for each service below.</p> <p>If you need help choosing your optimal Sanger service level, please contact us:</p> <h3> <a href="mailto:DNASeq@azenta.com"><em class="fa fa-envelope fa-icons blue-text"></em></a> <a href="mailto:DNASeq@azenta.com">Email</a> | <em class="fa fa-phone fa-icons blue-text"></em> Phone (1-877-436-3949, Ext. 2) | <a rel="noopener noreferrer" target="_blank" onclick="Velaro.Engagement.LoadPopoutChat()"><em class="fa fa-comment fa-icons blue-text"></em> Live Chat</a></h3> <div><a target="_blank" onclick="Velaro.Engagement.LoadPopoutChat()"><br /> </a></div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $("div[id^=Desc]").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Two-Column-Text-Image-LR-Layout-v1 Component-BackGround-Default pure-template divider margin-top-50 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="row"> <div class="col-md-7"> <h1 class="title-v2"><h3>Pre-Mixed</h3></h1> <p>For sequencing plasmids or purified PCR products, this service is the most economical choice.<br> <br> You provide your template and primer mixed in the same tube according to our <a href="/en/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines">Sample Submission Guidelines</a>.<br></p><br> <br> </div> <div class="col-md-5" id="DescImg"> <img src="/-/media/Icon_280x187_PreMixed.ashx?h=187&la=en&w=280&hash=D074646C0129423CBFB31C0A4C718BFD04D67A6D" alt="Building" width="280" height="187" /> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $("div[id^=Desc]").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Two-Column-Text-Image-LR-Layout-v1 Component-BackGround-Default pure-template divider margin-top-50 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="row"> <div class="col-md-7"> <h1 class="title-v2"><h3>Pre-Defined</h3></h1> <p>By choosing our Pre-Defined Service, you submit your template and primer in separate tubes, and a Azenta scientist will mix them when your sample is received. An additional charge applies in most cases.<br /> <br /> For sequencing plasmids or purified PCR products, submit your template and primer in separate tubes according to our <a href="/en/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines">Sample Submission Guidelines</a>.<br /> <br /> Note: An Azenta <a href="/en/Public/Resources/Free-Universal-Primers">Universal Primer</a> can be used in this service at no charge.</p> <br /> <br> </div> <div class="col-md-5" id="DescImg"> <img src="/-/media/Icon_280x187_PreDefined.ashx?h=187&la=en&w=280&hash=6718B43ECC9ECE08E2AA082BB0757427896A3772" alt="Building" width="280" height="187" /> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $("div[id^=Desc]").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Two-Column-Text-Image-LR-Layout-v1 Component-BackGround-Default pure-template divider margin-top-50 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="row"> <div class="col-md-7"> <h1 class="title-v2"><h3>Custom</h3></h1> <p>This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, an Azenta scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.<br> <br> Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.<br> <br> Submit template and primer separately according to our <a href="/en/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines">Sample Submission Guidelines</a>.<br> <br> Note: An Azenta <a href="/en/Public/Resources/Free-Universal-Primers">Universal Primer</a> can be used in this service at no charge.</p> <br> <br> </div> <div class="col-md-5" id="DescImg"> <img src="/-/media/Icon_280x187_Custom.ashx?h=187&la=en&w=280&hash=F88FCFFECADEC2732A32C03ED0F7F0E02180779E" alt="Building" width="280" height="187" /> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $("div[id^=Desc]").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Two-Column-Text-Image-LR-Layout-v1 Component-BackGround-Default pure-template divider margin-top-50 margin-bottom-30 padding-top-10 RenderingContext.Current.Rendering.Item.Fields["PaddingBottom"]'> <div class="container"> <div class="row"> <div class="col-md-7"> <h1 class="title-v2"><h3>Difficult Template Sequencing</h3></h1> <p>Revolutionize your sequencing experience with our Difficult Template sequencing service, specifically designed for challenging templates featuring GC-rich regions or intricate secondary structures like hairpins. With a success rate surpassing standard workflows by over double, researchers can now save at least a day in their process. Versatile and efficient, this protocol accommodates various DNA types without the need for additional templates, offering unparalleled convenience and reliability. <br /> <br /> Learn more about our proprietary Sanger sequencing service – <a href="https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/Purified-Templates">Difficult Template Sequencing</a></p> <br /> <br> </div> <div class="col-md-5" id="DescImg"> <img src="/-/media/Icon_280x187_DifficultTemplateSeq.ashx?h=187&la=en&w=280&hash=B522BCC807CF5A0F087B151B5D2DFEAFA9224C31" alt="Building" width="280" height="187" title="Difficult Template" /> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <h3>From Base Pairs to Breakthroughs: Understanding<br /> Sanger and Oxford Nanopore Sequencing</h3> <p>Monday, September 16, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02JnkHp0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Features & BENEFITS</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <h3>High Quality</h3> DNA sequencing read lengths ~1000 bases and at least 500 bases Phred20. A typical read will provide 800 bases Phred 20. Quality Score and Contiguous Read Length (CRL) provided in each read </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <h3>Convenient</h3> <a href="/en/Public/Company/Contact-Us">Contact us</a> to locate a drop box or pick-up service near you for free shipping </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em><a href="/?sc_mode=edit&sc_itemid={AE1AA216-DB79-4B80-9DC0-F6CC13A4C101}&sc_lang=en&sc_device=Mobile#"></a></div> <div class="content-boxes-in-v3"> <h3>Best-in-Class Difficult Template Protocols</h3> For hairpins and GC-rich DNA </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <h3>Free Vector Primers</h3> Wide selection of Azenta <a href="/en/Public/Resources/Free-Universal-Primers">Universal Primers</a> are available for most standard vectors </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <h3>Technical Support</h3> Available at no charge to discuss your project, troubleshoot, and answer questions 8 AM – 8 PM Eastern Time, Monday – Friday; results are posted on Saturdays for weekend workers </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <h3>Online System</h3> 24/7 web-based online ordering and data retrieval, with bar-coded order tracking </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <h3>Technology </h3> Analysis using ABI 3730xl DNA Analyzers for capillary electrophoresis and fluorescent dye terminator detection </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #ffffff; padding: 0; margin: 0;margin-top: -20px;"> <h2 style="border-bottom: 1px solid #f1f0f0;">TECHNICAL RESOURCES</h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Quick-Tips.ashx?h=160&w=220&la=en&hash=10DBA6E5F7939B892C9441028D5B83BD5B69682C" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Sanger Sequencing Quick Tips Guide</h3> <p>Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.<br /> • Volume 1: Producing Robust, Single-band PCR Product<br /> • Volume 2: PCR Clean-up of Single-band PCR <br /> • Volume 3: Salvaging Nonspecific PCR Products <br /> • Volume 4: PCR Visualization by Gel Electrophoresis<br /> • Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template <br /> <a href="https://web.genewiz.com/quick-tips-guide/sanger-sequencing" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Trouble-shooting-blog.ashx?h=160&w=220&la=en&hash=24FECB44F887ED8D2D363153D5842A55828C120E" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Troubleshooting DNA Templates with Sanger Sequencing</h3> <p>Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.<br /> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/ONT-Blog.ashx?h=160&w=220&la=en&hash=AFF32CFD100B581BA8F5F09D2DF7011B7407F301" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing</h3> <p>Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information. <br /> <a href="https://www.azenta.com/learning-center/blog/ensure-complete-plasmid-validation-your-first-attempt-oxford-nanopore-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Webinar--Sanger-ONT.ashx?h=160&w=220&la=en&hash=B46A4AA56DE351DD45E75213BD6FD1AA5AB8F799" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing</h3> <p>While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs. <br /> <a href="https://web.genewiz.com/workshop/base-pairs-to-breakthroughs" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Analyzing-Sanger-Blog.ashx?h=160&w=220&la=en&hash=8A9D75A95F2026E45E36D46F1028677640443783" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Analyzing Sanger Sequencing Data</h3> <p>The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data. <br /> <a href="https://www.azenta.com/learning-center/blog/analyzing-sanger-sequencing-data" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <p> </p> </div> </div> </div> <!--=== Process v1 ===--> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> <p>*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. 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