Sanger Sequencing | GENEWIZ from Azenta

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href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' 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href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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href="https://clims4.genewiz.com/CustomerHome/Index?lobId=1"><span style="color: #ffffff;">Place Order</span><br></a>   <a class="btn-u btn-u-lg rounded btn-u-blue" target="" href="/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines"><span style="color: #ffffff;">Sample Submission Guidelines</span><br /></a>    </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; padding: 0; } .sanger-new-container { background: #f0f0f0; padding: 30px 0; } .sanger-new-container h1 { margin: 0; color: #000000; } .sanger-new-content { width: 1170px; margin: 0 auto; } .sanger-new-content p { color: #000000; } .sanger-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #sanger-content-box-left { width: 60%; text-align: left; padding: 30px 0; } #sanger-content-box-right { width: 39%; text-align: right; } .sanger-content-box img { width: 400px; } .sanger-content-box button { border: none; border-radius: 4px; background: #0067B1; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .sanger-new-container { text-align: center; margin-top: 0; } .sanger-new-content { width: 90%; margin: 0 auto; } .sanger-new-content { display: inline-block; } #sanger-content-box-right, #sanger-content-box-left { width: 100%; text-align: center; } #sanger-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .sanger-new-content { width: 90%; margin: 0 auto; } .sanger-content-box img { width: 350px; } } </style> <div class="sanger-new-container"> <div class="sanger-new-content"> <div class="sanger-content-box" id="sanger-content-box-left"> <br /> <h1 style="text-transform: uppercase;">Sanger Sequencing</h1> <div> </div> <p>Sanger Sequencing is a cost-effective method for determining the nucleotide sequence of DNA. GENEWIZ Sanger sequencing services are award-winning, providing high-quality results, industry-leading customer service and fast turnaround times at competitive prices. GENEWIZ from Azenta Life Sciences is the partner of choice for academic, pharmaceutical, GLP, biotechnology, and government institutions around the world. </p> <p>We are well-equipped to handle DNA sequencing of all complexities including single tube, high-throughput, direct bacterial colony, glycerol stock, CLIA, or GLP DNA sequencing projects. Our mission is to eliminate bottlenecks, improve productivity, and advance your research progress.</p> </div> <div class="sanger-content-box" id="sanger-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02mj2kW0" target="_blank"><img alt="" src="/-/media/Sanger-50-Off-First-Order.ashx?la=en&hash=81E1D55079527BF3CEF0E873B371A7E2C8FC05E3" /></a> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>GENEWIZ Sanger Sequencing Service</h2> <p><span style="color: #595959;">Azenta accepts a variety of different templates to use as starting material for Sanger sequencing reactions. <br> Click on the service below to view the service options available for each starting material.</span><span style="color: #595959;"><br> </span></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4 col-xs-12'> <a href='https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/Sanger-EZ' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Icon-purple-210x110-Sanger-EZ.ashx?h=110&la=en&w=210&hash=DD773098411986976A9928F428E191C65E6B341E" alt="Building" width="210" height="110" title="Sanger-EZ Icon" /> </div> <div class="row"> <h3>Sanger-EZ</h3> <br /> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_DifficultTemplateSeq.ashx?h=110&la=en&w=210&hash=58F3E964BE77C611D557F29C1BDE56A3C3DD3E21" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Difficult Template <br /> Sequencing</h3> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/Purified-Templates' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_PurifiedProducts.ashx?h=110&la=en&w=210&hash=952DDEC71C866FC1CBAE9BECBCB7ADF667512EC8" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Purified Products: <br> Plasmids or PCR products</h3> </div> <div class="row"> <br> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_DirectColonySeq.ashx?h=110&la=en&w=210&hash=3FCC9EBDFB996D05E974327409E747DA81B1CFCB" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Direct Colony Sequencing</h3> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='/Public/Services/Sanger-Sequencing/PCR-Purification' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_PCRPurification.ashx?h=110&la=en&w=210&hash=6CECA6295342D6B9A688094D00CA7C5488DD83E2" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>PCR Purification</h3> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='/en/Public/Services/Sanger-Sequencing/Premium-Services' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_PrimerWalking.ashx?h=110&la=en&w=210&hash=FE459FCC9C8BFE3CE39B7F06BDC8D96DB42ECC76" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Primer Walking</h3> <br /> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_CLIA.ashx?h=110&la=en&w=210&hash=7248F87A4C2D7789380A742D88537EE9292D240A" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>CLIA</h3> <br /> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/AAV-ITR' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Icon_210x110_ITR-Seq.ashx?h=110&la=en&w=211&hash=9210AB6E5F3E183A1A132BDDDBD79F316A9763E9" alt="Building" width="211" height="110" /> </div> <div class="row"> <h3>ITR Sequencing</h3> <br /> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4 col-xs-12'> <a href='https://hubs.ly/Q02kXXzr0' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/50008-D_Low-Conc-Plasmids_GW-Icon_210x110.ashx?h=110&la=en&w=210&hash=389FDB1D1608BD94BAC399D2CBB97F35356DBC4B" alt="Building" width="210" height="110" title="Low Concentration Plasmid Icon" /> </div> <div class="row"> <h3>Low Concentration Plasmid Sequencing</h3> <br /> <br /> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> <h2 style="text-align: center;">REGULATORY SANGER SEQUENCING</h2> <p><span style="color: #595959;">Standalone Sanger sequencing services are also offered at the GLP-compliant level. For more information, please email</span> <a href="mailto:Regulatory@azenta.com">Regulatory@azenta.com</a></p> <br /> <br /> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11038-D_PEZ-DirectColonySeq_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 15px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner1 { width: 100%; height: auto; } .covid-content1 { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <br /> <h3><span style="color: #55c8d2;">NEW</span> Direct Colony to<br /> Whole Plasmid Sequencing</h3> <a href="https://hubs.ly/Q02FKvPQ0" target="_blank"> <br /> <button> LEARN MORE</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Gray margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Sanger Sequencing Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>High Quality</strong> - DNA sequencing read lengths up to ~1000 bases.A typical read will provide 800 bases Phred20.  Quality Score and Contiguous Read Length (CRL) provided in each read.<br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Azenta Technical Support</strong> - Our highly-trained team of Sanger sequencing specialists proactively communicate updates, while providing technical expertise on projects big and small. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Convenient</strong> - <a href="/en/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines"><a href="/en/Public/Company/Contact-Us">Contact us</a></a> to locate a drop box or pick-up service near you for free shipping. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Easy, Online Ordering</strong> - 24/7 web-based online ordering and data retrieval, with bar-coded order tracking make it easy to place your order and view data. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Best-in-Class Difficult Template Protocols</strong> - Our proprietary technologies allow for consisent, reliable data even on the most difficult templates (including hairpins and GC-rich DNA). </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Free Vector Primers</strong> – a wide selection of <a href="/en/Public/Resources/Free-Universal-Primers">Azenta Universal Primers</a> are available for most standard vectors. Also visit our Universal Primer Selection Tool to determine which Universal Primer binds to your vector of choice. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <div class="process-v1 Component-BackGround-Default pull-center margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="row text-center"> <h2>Sanger Sequencing Protocol</h2> <p><span style="color: #595959;">* Samples must arrive at the Azenta New Jersey laboratory before 10:00 am EST to qualify for Same Day service.<br /> Note that direct-sequencing templates are not available for our Same Day service.</span></p> </div> <div class="row "> <div class="col-sm-4"> <img alt="Same Day Turnaround Time" style="height: 110px; width: 210px;" src="/-/media/Images/Icon_210x110_FastTurnaround.ashx?la=en&hash=715CE4176251C6CCD853ED4ED8DB283E461F1339" /> <h3><span style="color: #595959;">Same Day</span></h3> <p><span style="color: #595959;">FOR RUSH PROJECTS: Data is reported by 7PM Eastern the same day we receive samples. A small additional charge applies. Please contact <a href="mailto:dnaseq@genewiz.com">Technical Support</a> for additional information.</span></p> </div> <div class="col-sm-4"> <img alt="Overnight Local Service Turnaround Time" style="height: 110px; width: 210px;" src="/-/media/Icon_210x110_Overnight-TAT.ashx?la=en&hash=2BF6CFBFC0E60AEE0B2159725EAAABCAE357C429" /> <h3><span style="color: #595959;">Overnight Local Service</span></h3> <p><span style="color: #595959;">Overnight service for customers near a Azenta laboratory. Laboratories are located in New York City, Boston, Washington D.C. Metro Area, Research Triangle Park, San Diego, Los Angeles Metro Area, San Francisco Bay Area, and Seattle. Typically samples are picked up in the afternoon by a Azenta courier, and data are reported the next morning. Please <a href="/en/Public/Company/Contact-Us">contact us</a> for details and eligibility.</span></p> </div> <div class="col-sm-4"> <img alt="Standard 24-Hour Turnaround Time" style="height: 110px; width: 210px;" src="/-/media/Icon_210x110_StandardTurnaround.ashx?h=110&w=210&la=en&hash=DC17D6A92DDEB4D039E8090635F096C4CD6C2517" /> <h3><span style="color: #595959;">Standard 24-Hour</span></h3> <p><span style="color: #595959;">Data reported within one business day after samples are received by Azenta.</span></p> </div> </div> </div> </div> <br /> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/15019-D_Boost-Plasmid-Prep_WebBanFull-Lrg_Background.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner3-left { 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