Multiomic Insights with RNAscope ISH and IHC

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id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p lang="EN-US"><a href="#identify-more">Identify More</a> | <a href="#gallery">Image Gallery</a> | <a href="#choose-what-works-for-you">Choose What Works For You</a> | <a href="#testimonials">From Your Peers</a> | <a href="#resources">Resources</a></p><p> </p><h2>Why Visualize RNA and Protein Together?</h2><p> </p><p>Understanding the spatial relationship between RNA and protein is crucial for gaining a holistic view of cellular processes. However, analyzing these molecules separately, as has been done traditionally, misses the intricate interplay that defines tissue function and disease progression. Spatial Multiomics examines RNA and proteins in relation to tissue morphology enabling a complete understanding of gene expression regulation and protein function within specific cellular contexts.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="gallery"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="identify-more"></a></p><h2>Identify More </h2></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/rnascope-multiomics-application-cervical-cancer.jpg" alt="RNAscope™ Fluorescent Multiplex v2 Assay: FFPE tissue, human cervical cancer - CD3 protein (green) ifng RNA (pink), cxcl9 RNA (white)" loading="lazy" > </div> <h3 class="card_title_htag"> <div><h3>Secreted Protein and its Origin </h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>RNAscope Multiplex V2 assay for studying Cytotoxic T lymphocytes in cervical cancer tissue. T cells were identified using protein marker CD3 while cytokine markers <em>IFNG </em>and <em>CXCL9 </em>were detected using RNA.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/rnascope-colon-inflamation-image-gallery-image1-v2.jpg" alt="Detection of cytokines, immune and tumor cells using an RNAscope RNA-protein multiomic panel of 4 marker proteins, CD4 (yellow), CD8 (white), PanCK (pink), FoxP3 (green), and 2 target RNA probes, IFNG (red), GZMB (turquoise) in human colon cancer tissue" loading="lazy" > </div> <h3 class="card_title_htag"> <div><h3>Characterize Immune Cells in the Tumor Microenvironment </h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Visualizing TIL activation in Colon cancer. Using codetection to identify tumor infiltrating regulatory T cells marked by CD4+/FOXP3+ , Cytotoxic T lymphocytes seen as CD8+/ IFNG+. </p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/rnascope-multiomics-application-visualize-changes-in-cell-state.JPG" alt="RNAscope multiomics image Mapping tumor associated M1/M2 macrophages marked by CD163+ / CD68+ , IL1B+ / IL10+ in cervical cancer tissue" loading="lazy" > </div> <h3 class="card_title_htag"> <div><h3>Visualize Changes in Cell State</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Mapping tumor associated M1/M2 macrophages marked by CD163+ / CD68+ , IL1B+ / IL10+ in cervical cancer tissue</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> 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<div><p>Leverage the robustness of RNAscope technology by combining in situ hybridization and IHC protocol in the same sample slide leveraging the sequential workflow for both manual and automated platforms from both Leica Biosystems and Roche Tissue Diagnostics.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/rnascope-ish-technology/rnascope/pretreat-pro-protease-free" > Learn About Manual Protease-free Workflows </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2> Search publications utilizing RNAscope with antibodies for multiomic studies</h2></div> <a href="/resources/rnascope-citations" > <button type="button" class="btn btn-primary"> Find RNAscope Citations </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="testimonials"></a></p></div> </div> </div> <div> <div 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expression are all critical for evaluating the pathogenesis of HIV infection and for developing cure strategies to eliminate HIV reservoirs. RNAscope in situ hybridization (ISH) has enabled us to visualize and quantify HIV RNA with single-copy resolution. 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This new service enables researchers to detect up to 12 protein or RNA targets in any combination. </p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/webinars/spatial-multiomics-with-same-section-detection-of-12-proteins-or-RNAs" class="btn btn-primary" type="link"> Watch Webinar </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://origin.bio-techne.com/resources/literature/rnascope-automated-ls-assay-brochure" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/MK51183_RevA_Expand_Capabilities_with_RNAscope_and_Leica_BONDRX.jpg" alt="RNAscope Automated LS Assay - Expand your capabilities with RNAscope ISH Assays on the BOND RX from Leica biosystems" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://origin.bio-techne.com/resources/literature/rnascope-automated-ls-assay-brochure" > <h3 class="card_title_htag"> <div><h3>RNAscope™ Automated LS Assay on the BOND RX from Leica Biosystems</h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="https://origin.bio-techne.com/resources/literature/rnascope-automated-ls-assay-brochure" > Download Flyer </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/automated-multiomics-on-comet-flyer.pdf" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/comet-multiomics-brochure.JPG" alt="Image of the COMET multiomics brochure" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/automated-multiomics-on-comet-flyer.pdf" > <h3 class="card_title_htag"> <div><h3>COMET Multiomics Brochure </h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/automated-multiomics-on-comet-flyer.pdf" > Download Flyer </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/combining-rnascope-ish-with-ihc-to-spatially-resolve-rna-and-protein-targets-simultaneously-app-note" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/combining-rna-ish-ihc-app-note.jpg" alt="Combining the RNAscope ISH Technology with IHC to spatially resolve RNA and protein targets simultaneously" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/combining-rnascope-ish-with-ihc-to-spatially-resolve-rna-and-protein-targets-simultaneously-app-note" > <h3 class="card_title_htag"> <div><h3>Combining RNAscope ISH Technology with IHC</h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/combining-rnascope-ish-with-ihc-to-spatially-resolve-rna-and-protein-targets-simultaneously-app-note" > Download App Note </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/banners/v8_round-black-blue-gradient-banner-1110x350.png" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>Get Started Today. 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Multiomic Insights with RNAscope ISH and IHC