DIC | Nikon’s MicroscopyU

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The technique produces a monochromatic shadow-cast image that effectively displays the gradient of optical paths for both high and low spatial frequencies present in the specimen. Those regions of the specimen where the optical paths increase along a reference direction appear brighter (or darker), while regions where the path differences decrease appear in reverse contrast. As the gradient of optical path difference grows steeper, image contrast is dramatically increased.</p></div> <section class="articlelist" id="rev"> <h2>Review Articles</h2> <ul> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/dic/de-sénarmont-bias-retardation-in-dic-microscopy">de Sénarmont Bias Retardation in DIC Microscopy</a></h3> <p>DIC with a fixed Nomarski prism and a simple de Sénarmont compensator.</p> </li> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/dic/de-sénarmont-dic-microscope-configuration">de Sénarmont DIC Microscope Configuration</a></h3> <p>de Sénarmont compensators offer more accuracy for introduction of bias retardation.</p> </li> </ul> <ul> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/dic/reflected-light-dic-microscopy">Reflected Light DIC Microscopy</a></h3> <p>Examination of highly reflective specimens in DIC microscopy with epi-illumination.</p> </li> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/dic/specimen-contrast-in-optical-microscopy">Specimen Contrast in Optical Microscopy</a></h3> <p>Using phase-related optical techniques to increase specimen contrast.</p> </li> </ul> <hr /> </section> <section class="thumblist tutoriallist" id="tuts"> <h2>Interactive Tutorials</h2> <ul> <li> <a href="https://www.microscopyu.com/tutorials/bias-retardation-effects-on-specimen-contrast"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/thumb-biasretardation.jpg" height="80" width="80" /> <h3>Bias Retardation Effects on Specimen Contrast</h3></a> <p>Explore the effects of varying bias retardation on specimen contrast.</p> </li> <li> <a href="https://www.microscopyu.com/tutorials/comparison-of-phase-contrast-and-dic-microscopy"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/Phase-DIC.jpg" height="80" width="80" /> <h3>Comparison of Phase Contrast & DIC Microscopy</h3></a> <p>Examples of the same specimen viewed in either phase contrast or DIC.</p> </li> <li> <a href="https://www.microscopyu.com/tutorials/nomarski-prism-action-in-polarized-light"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/nomarski.jpg" height="80" width="80" /> <h3>Nomarski Prism Action in Polarized Light</h3></a> <p>Variations in prism geometry yield unique interference patterns in polarized light. </p> </li> <li> <a href="https://www.microscopyu.com/tutorials/optical-sectioning-in-reflected-light-dic"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/optical-sectionin-reflected-light.jpg" height="80" width="80" /> <h3>Optical Sectioning in Reflected Light DIC</h3></a> <p>Optical sectioning of reflected light specimens (semiconductors). </p> </li> </ul> <ul> <li> <a href="https://www.microscopyu.com/tutorials/optical-sectioning-with-de-sénarmont-dic-microscopy"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/de-sernamont.jpg" height="80" width="80" /> <h3>Optical Sectioning with de Sénarmont DIC Microscopy</h3></a> <p>At high numerical apertures, DIC can be used for optical sectioning.</p> </li> <li> <a href="https://www.microscopyu.com/tutorials/wavefront-relationships-in-reflected-light-dic-microscopy"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/wavefront-reflected-light-dic.jpg" height="80" width="80" /> <h3>Wavefront Relationships in Reflected Light DIC Microscopy</h3></a> <p>Observe how light waves travel through a reflected light DIC microscope. </p> </li> <li> <a href="https://www.microscopyu.com/tutorials/wavefront-relationships-in-de-sénarmont-and-nomarski-dic"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/wavefront-relatioships-de-senarmont.jpg" height="80" width="80" /> <h3>Wavefront Relationships in de Sénarmont and Nomarski DIC</h3></a> <p>An interactive comparison of wavefronts in these complementary techniques. </p> </li> </ul> <hr /> </section> <section id="gals"> <h2>Galleries</h2> <div class="gallerycollection"> <div class="galleryblurb"> <a href="https://www.microscopyu.com/galleries/dic-phase-contrast"> <h3>Differential Interference Contrast (DIC)</h3> <img src="https://www.microscopyu.com/assets/poster-images/_galleryThumbnailSquare/DIC-gallery-poster.jpg" height="80" width="80" /> </a> <p>Compare specimen contrast using these complementary imaging techniques.</p> <p class="gallerycontents">Image Comparisons • Movies</p> </div> <hr /> </div> </section> <section class="referencelist" id="refs"> <h2>Selected Literature References</h2> <ul> <li><h3><a href="https://www.microscopyu.com/references/dic">Differential Interference Contrast (DIC)</a></h3> <p>Contrast enhancement using interference of polarized light wavefronts.</p> </li> </ul> <ul> <li><h3><a href="https://www.microscopyu.com/references/specimencontrast">Specimen Contrast in Microscopy</a></h3> <p>Examine the origins of contrast in a wide spectrum of specimens.</p> </li> </ul> <hr /> </section> <div id="footnotes"> <h2>Contributing Authors</h2> <p><strong>Douglas B. Murphy</strong> - Department of Cell Biology and Anatomy and Microscope Facility, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, 107 WBSB, Baltimore, Maryland 21205.</p> <p><strong>Stanley A. Schwartz</strong> - Nikon Instruments, Inc., 1300 Walt Whitman Road, Melville, New York, 11747.</p> <p><strong>Edward D. Salmon</strong> - Department of Cell Biology, The University of North Carolina, Chapel Hill, North Carolina 27599.</p> <p><strong>Kenneth R. Spring</strong> - Scientific Consultant, Lusby, Maryland, 20657.</p> <p><strong>Matthew Parry-Hill</strong>, <strong>Robert T. Sutter</strong>, and <strong>Michael W. Davidson</strong> - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.</p> </div> <div class="sharebar"> <p>Share this page:</p> <ul class="share-buttons"> <li><a href="https://www.facebook.com/sharer/sharer.php?u=http%3A%2F%2Fwww.microscopyu.com&t=" title="Share on Facebook" target="_blank" onclick="window.open('https://www.facebook.com/sharer/sharer.php?u=' + encodeURIComponent(document.URL) + '&t=' + encodeURIComponent(document.URL)); return false;"><img src="/images/social_flat_rounded_rects_svg/Facebook.svg"></a></li> <li><a href="https://twitter.com/intent/tweet?source=http%3A%2F%2Fwww.microscopyu.com&text=:%20http%3A%2F%2Fwww.microscopyu.com" target="_blank" title="Tweet" onclick="window.open('https://twitter.com/intent/tweet?text=' + encodeURIComponent(document.title) + ':%20' + encodeURIComponent(document.URL)); return false;"><img src="/images/social_flat_rounded_rects_svg/Twitter.svg"></a></li> <li><a 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